Rh1DG232900

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000671
Physical Location & Seq
Forward (+)
44601248 .. 44608415
7168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG232900.1

Sequence Viewer

Length: 3588 bp
ATGGCTGCGATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAGGTCTGTTCAGAGCTGGAAACTCATCTAGGGTTTGGGGACAAAGTTCTAGCTGAGTTCATAACTGAGATGGGACGAAACTGTGAGAGTGTGGATGAATTCGATGCCAAATTGAAGGAACACGGTGCCGAGATGCCTGATTACTTTGTCCGTACACTCCTCACTATCATTCACGCAATTCTTCCTCCTAAGGCAAAGTCCGAGAACGACTCAAAGAAAGAGAGCACTGCTGAAGGTAGTAAAAGCAAGTTCAAGGCTTTGTCTATTGCTGATAACAGGGATAGGGTAAAGGATATCCAGAGGGAAATTGAGTTGGAGGCCAAGGAAAAGCGAAATGACAGAGAAGAATGGGGAGATGGGCGTGAAGAAGATAGGCCCAGAGGTAGAGATGGAGGTAGAGGTGGGGGTAGGGAAAGAGATACAAATCGAGACAGGGACAGGGACAGGGACAGGGATAGGAGAGAGAGGGGAAGGGATAGGTATGATAGAGATGAAAGGCGGAGAGATAATCATTATCATGATTATGATAAGGGAAGGCATACAGATCGATCCAATAAGCACAGGAGGGATGAGTATGAAAAGGATGGAGATGTTAGAGAAGATGATGTTGATAGGAGAGGAAGTAGGGATCATCAGAATGGATTGCACCATTCTGATGAGCCTGAATTGTATAAGGTTTATAAGGGTAGGGTCTCAAAAGTGATGGACACAGGTTGCTTTGTTCAGTTCAGCGATTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCTACTCGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAAGAGGTCTATGTTAAGGTGATTTCAATTTCTGGTCAAAAGTTGAGCCTGTCAATGAGGGATGTTGATCAGCATACTGGGAAGGATTTGCTACCTTTGAAGAAGAACTCAGAGGATGATTCTCTTAGGACAAACCCGTCGATATCAAAGGATGATGGGCCTGTGACAAGGACGGGTCTCTCTGGGATTAGGATTGTGGAAGAGGATGTTACTGCCCCATCTCGCCGGCCATTGAAGAGAATGAGCTCACCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCGGGTGTTTTGGGTGTCAAAGAGTATCCAATGTATGATGAAGAAACAGATGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAACTTGAGATTGAGCTTAATGAGGATGAGCCAGCCTTCTTGCAAGGGCAAACCAGGTATTCCGCAGATATGTCACCTGTGAAAATATTTAAGAATCCAGAAGGGTCATTGAGTCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACCGTCACTTTTGGGCAGAGGTCAAAGCTCTCAATTCAGGAACAGAGGCAGAGCTTGCCTATCTATAAGCTGAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCAGGCAAGACAACCCAGGTAACGCAGTATCTTGCAGAAGCTGGATACACGACAATGGGTAAGATTGGGTGTACACAGCCACGTAGGGTGGCTGCGATGTCTGTAGCCAAGAGGGTTGCTGAAGAGTTTGGTTGTCGTCTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGACCTGATACTGTAATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTCTGCTTGACGAGAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAACTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTCACATCTGCTACCTTGGATGCCGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATCCCCGGAAGAACTTTTCCTGTAGAGATACTCTACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTTTAATCACTGTTCTACAAATTCACTTAACGGAGCCGGAAGGTGACATCCTTCTCTTTTTGACGGGACAGGAAGAAATTGATTTTGCATGCCAGTCACTATATGAGAGGATGAAAGGTCTTGGTAAAAATGTGCCTGAGTTGATTATCTTACCAGTTTATAGTGCCCTTCCCAGTGAAATGCAGTCGAGGATATTTGATCCAGCCCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACCAATATAGCCGAGGCATCACTGACCATCGATGGGATATTTTATGTAATTGACCCTGGATTTGCAAAGCAAAATGTTTATAACGCAATGCAGGGGCTAGATTCACTAGTCATAACTCCAATATCACAAGCATCGGCCAAGCAACGAGCTGGGCGTGCTGGCCGTACAGGGCCTGGGAAATGTTACCGCCTTTACACTGAGAGTGCATACCGCAATGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGACTATTACATTAACGATGAAAGCAATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGGATCCTCCTTCACCCCAAGCACTCATTTCTGCTATGGAACAACTATACAGTTTAGGAGCCCTAGATGAAGAGGGTCTTCTGACCAAATTGGGTAGGAAAATGGCCGAGTTTCCTCTGGATCCACCATTATCTAAGATGCTACTGGCCAGCGTGGACCTTGGATGCAGTGATGAGATCCTGACTATCATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAGCAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGATCATCTGACTTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAACTTTTCGGGGCCTTGGTGTTTTGAGAACTTTGTTCAGTCTCGATCCCTTAGGAGGGCACAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGACGTTGTGAGTGCTGGAAAGAATTTTACAAAGATCAGGAAGGCAATTACTGCAGGCTTTTTCTTCCATGCTGCTAGGAAGGACCCACAGGAGGGGTATAGAACCCTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCTCTCTTCCAGAGACAACCGGATTGGGTCATCTACCATGAGTTGGTTATGACTACAAAGGAGTATATGCGGGAGGTGACAGTCGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCACGGTGGCTGATCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAACGTATTGAACCGCTATATGACAGATACCATGAACCAAACTCCTGGCGTCTTAGTAAACGGCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1195

Amino Acids

136.36

Weight (kDa)

6.29

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 243 - 310 4.3e-11 S1 RNA binding domain
DEAD PF00270 549 - 698 1e-07 DEAD/DEAH box helicase
AAA_22 PF13401 557 - 690 1.6e-06 AAA domain
Helicase_C PF00271 742 - 872 1.6e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 934 - 962 8.3e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 963 - 1022 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1080 - 1156 1e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 735, 2526
AasI GACNNNNNNGTC 1 cut(s) 1009
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1481, 3391
AciI CCGC 7 cut(s) 553, 1302, 2050, 2632, 2656, 3418, 3530
AcoI YGGCCR 5 cut(s) 1100, 2580, 2605, 2871, 2913
AcsI RAATTY 4 cut(s) 152, 2229, 2688, 3226
AcuI CTGAAG 4 cut(s) 306, 1661, 1845, 2661
AcyI GRCGYC 1 cut(s) 3565
AfaI GTAC 6 cut(s) 38, 208, 1777, 1910, 1967, 2611
AhlI ACTAGT 1 cut(s) 2551
AjnI CCWGG 8 cut(s) 1292, 1719, 2419, 2500, 2617, 3009, 3464, 3560
AjuI GAANNNNNNNTTGG 4 cut(s) 791, 823, 1634, 1666
Alw21I GWGCWC 3 cut(s) 281, 1121, 3352
Alw26I GTCTC 8 cut(s) 477, 751, 1055, 1472, 1691, 2047, 3141, 3355
AlwNI CAGNNNCTG 3 cut(s) 1481, 1745, 2618
Ama87I CYCGRG 2 cut(s) 32, 1156
ApeKI GCWGC 8 cut(s) 5, 1358, 1361, 1408, 1411, 1796, 2182, 3275
ApoI RAATTY 4 cut(s) 152, 2229, 2688, 3226
ArsI GACNNNNNNTTYG 4 cut(s) 2046, 2078, 2489, 2521
Asp700I GAANNNNTTC 1 cut(s) 1653
AspA2I CCTAGG 1 cut(s) 2993
AspLEI GCGC 1 cut(s) 3507
AspS9I GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
AsuC2I CCSGG 1 cut(s) 2145
AsuHPI GGTGA 9 cut(s) 874, 904, 1113, 1305, 1496, 1706, 2264, 2772, 3436
AvaI CYCGRG 2 cut(s) 32, 1156
AvaII GGWCC 3 cut(s) 1889, 2923, 3286
AvrII CCTAGG 1 cut(s) 2993
AxyI CCTNAGG 2 cut(s) 243, 3146
BaeGI GKGCMC 2 cut(s) 2377, 3157
BalI TGGCCA 1 cut(s) 2915
BamHI GGATCC 2 cut(s) 2770, 2887
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 1121, 2830
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 1574, 2837
Bbv12I GWGCWC 3 cut(s) 281, 1121, 3352
BbvCI CCTCAGC 1 cut(s) 1233
BbvI GCAGC 7 cut(s) 1348, 1370, 1420, 1423, 1783, 2194, 3262
BceAI ACGGC 1 cut(s) 2592
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
BciVI GTATCC 2 cut(s) 1191, 1742
BclI TGATCA 2 cut(s) 940, 3016
BcnI CCSGG 1 cut(s) 2145
BcoDI GTCTC 8 cut(s) 477, 751, 1055, 1472, 1691, 2047, 3141, 3355
BcuI ACTAGT 1 cut(s) 2551
BfaI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
BfmI CTRYAG 3 cut(s) 1806, 2160, 3255
BfuI GTATCC 2 cut(s) 1191, 1742
BisI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
BlnI CCTAGG 1 cut(s) 2993
BlsI GCNGC 8 cut(s) 7, 1360, 1363, 1410, 1413, 1798, 2184, 3277
BmcAI AGTACT 1 cut(s) 1967
Bme1390I CCNGG 9 cut(s) 1294, 1721, 2145, 2421, 2502, 2619, 3011, 3466, 3562
Bme18I GGWCC 3 cut(s) 1889, 2923, 3286
BmeT110I CYCGRG 2 cut(s) 32, 1156
BmgT120I GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
BmiI GGNNCC 7 cut(s) 181, 2244, 2772, 2827, 2889, 3108, 3288
BmrFI CCNGG 9 cut(s) 1294, 1721, 2145, 2421, 2502, 2619, 3011, 3466, 3562
BmrI ACTGGG 2 cut(s) 960, 2376
BmuI ACTGGG 2 cut(s) 960, 2376
BpiI GAAGAC 2 cut(s) 1574, 2837
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu10I CCTNAGC 1 cut(s) 1233
BpuEI CTTGAG 1 cut(s) 1265
BpuMI CCSGG 1 cut(s) 2145
Bsa29I ATCGAT 2 cut(s) 601, 2475
BsaAI YACGTR 1 cut(s) 1787
BsaBI GATNNNNATC 3 cut(s) 477, 939, 1449
BsaHI GRCGYC 1 cut(s) 3565
BsaI GGTCTC 3 cut(s) 751, 1055, 2047
BsaWI WCCGGW 2 cut(s) 1123, 3367
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bse118I RCCGGY 1 cut(s) 1098
Bse21I CCTNAGG 2 cut(s) 243, 3146
Bse3DI GCAATG 4 cut(s) 2538, 2665, 2739, 2955
Bse8I GATNNNNATC 3 cut(s) 477, 939, 1449
BseBI CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
BseCI ATCGAT 2 cut(s) 601, 2475
BseJI GATNNNNATC 3 cut(s) 477, 939, 1449
BseMI GCAATG 4 cut(s) 2538, 2665, 2739, 2955
BseMII CTCAG 8 cut(s) 99, 111, 996, 1224, 1533, 1976, 2337, 2634
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 2 cut(s) 2377, 3157
BseXI GCAGC 7 cut(s) 1348, 1370, 1420, 1423, 1783, 2194, 3262
BseYI CCCAGC 1 cut(s) 2594
BsgI GTGCAG 3 cut(s) 1347, 1359, 1377
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 2 cut(s) 601, 2475
BsiHKAI GWGCWC 3 cut(s) 281, 1121, 3352
BsiHKCI CYCGRG 2 cut(s) 32, 1156
BsiSI CCGG 5 cut(s) 1099, 1124, 2145, 2246, 3368
BslFI GGGAC 8 cut(s) 107, 141, 503, 509, 515, 2289, 2653, 2722
BsmAI GTCTC 8 cut(s) 477, 751, 1055, 1472, 1691, 2047, 3141, 3355
BsmFI GGGAC 8 cut(s) 107, 141, 503, 509, 515, 2289, 2653, 2722
Bso31I GGTCTC 3 cut(s) 751, 1055, 2047
BsoBI CYCGRG 2 cut(s) 32, 1156
Bsp1286I GDGCHC 6 cut(s) 281, 1121, 2377, 2830, 3157, 3352
Bsp1407I TGTACA 1 cut(s) 1775
BspACI CCGC 7 cut(s) 553, 1302, 2050, 2632, 2656, 3418, 3530
BspCNI CTCAG 8 cut(s) 100, 112, 995, 1225, 1534, 1975, 2338, 2635
BspDI ATCGAT 2 cut(s) 601, 2475
BspHI TCATGA 2 cut(s) 571, 1993
BspLI GGNNCC 7 cut(s) 181, 2244, 2772, 2827, 2889, 3108, 3288
BspMAI CTGCAG 1 cut(s) 3259
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 3 cut(s) 751, 1055, 2047
BsrDI GCAATG 4 cut(s) 2538, 2665, 2739, 2955
BsrFI RCCGGY 1 cut(s) 1098
BsrGI TGTACA 1 cut(s) 1775
BssAI RCCGGY 1 cut(s) 1098
BssNI GRCGYC 1 cut(s) 3565
BssT1I CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
Bst2UI CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
Bst6I CTCTTC 9 cut(s) 860, 1068, 1103, 1218, 1821, 2420, 2832, 3017, 3359
BstACI GRCGYC 1 cut(s) 3565
BstAPI GCANNNNNTGC 2 cut(s) 1624, 3254
BstAUI TGTACA 1 cut(s) 1775
BstBAI YACGTR 1 cut(s) 1787
BstHHI GCGC 1 cut(s) 3507
BstMAI GTCTC 8 cut(s) 477, 751, 1055, 1472, 1691, 2047, 3141, 3355
BstMWI GCNNNNNNNGC 5 cut(s) 1408, 1624, 2600, 3089, 3254
BstNI CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
BstNSI RCATGY 2 cut(s) 1540, 2301
BstSCI CCNGG 9 cut(s) 1292, 1719, 2143, 2419, 2500, 2617, 3009, 3464, 3560
BstSFI CTRYAG 3 cut(s) 1806, 2160, 3255
BstSLI GKGCMC 2 cut(s) 2377, 3157
BstV1I GCAGC 7 cut(s) 1348, 1370, 1420, 1423, 1783, 2194, 3262
BstV2I GAAGAC 2 cut(s) 1574, 2837
BstX2I RGATCY 5 cut(s) 1444, 1465, 2770, 2887, 2943
BstXI CCANNNNNNTGG 1 cut(s) 3561
BstYI RGATCY 5 cut(s) 1444, 1465, 2770, 2887, 2943
Bsu15I ATCGAT 2 cut(s) 601, 2475
Bsu36I CCTNAGG 2 cut(s) 243, 3146
BsuI GTATCC 2 cut(s) 1191, 1742
BsuTUI ATCGAT 2 cut(s) 601, 2475
BtgZI GCGATG 1 cut(s) 1814
BtsI GCAGTG 4 cut(s) 279, 1668, 2941, 3352
BtsIMutI CAGTG 9 cut(s) 279, 1668, 1884, 2217, 2389, 2465, 2640, 2941, 3352
CaiI CAGNNNCTG 3 cut(s) 1481, 1745, 2618
CciI TCATGA 2 cut(s) 571, 1993
CfoI GCGC 1 cut(s) 3507
Cfr10I RCCGGY 1 cut(s) 1098
Cfr13I GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
ClaI ATCGAT 2 cut(s) 601, 2475
CseI GACGC 2 cut(s) 3500, 3554
CsiI ACCWGGT 1 cut(s) 1292
Csp6I GTAC 6 cut(s) 37, 207, 1776, 1909, 1966, 2610
CviQI GTAC 6 cut(s) 37, 207, 1776, 1909, 1966, 2610
DrdI GACNNNNNNGTC 1 cut(s) 1009
DseDI GACNNNNNNGTC 1 cut(s) 1009
EaeI YGGCCR 5 cut(s) 1100, 2580, 2605, 2871, 2913
Eam1104I CTCTTC 9 cut(s) 860, 1068, 1103, 1218, 1821, 2420, 2832, 3017, 3359
EarI CTCTTC 9 cut(s) 860, 1068, 1103, 1218, 1821, 2420, 2832, 3017, 3359
EciI GGCGGA 1 cut(s) 568
Ecl136II GAGCTC 1 cut(s) 1119
Eco130I CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
Eco147I AGGCCT 1 cut(s) 2992
Eco24I GRGCYC 2 cut(s) 1121, 2830
Eco31I GGTCTC 3 cut(s) 751, 1055, 2047
Eco32I GATATC 2 cut(s) 349, 1017
Eco47I GGWCC 3 cut(s) 1889, 2923, 3286
Eco53kI GAGCTC 1 cut(s) 1119
Eco57I CTGAAG 4 cut(s) 306, 1661, 1845, 2661
Eco81I CCTNAGG 2 cut(s) 243, 3146
Eco88I CYCGRG 2 cut(s) 32, 1156
EcoICRI GAGCTC 1 cut(s) 1119
EcoO109I RGGNCCY 3 cut(s) 2615, 3107, 3286
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 8 cut(s) 1292, 1719, 2419, 2500, 2617, 3009, 3464, 3560
EcoRV GATATC 2 cut(s) 349, 1017
EcoT14I CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
EcoT22I ATGCAT 1 cut(s) 2209
EcoT38I GRGCYC 2 cut(s) 1121, 2830
ErhI CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
FalI AAGNNNNNCTT 2 cut(s) 2829, 2861
FaqI GGGAC 8 cut(s) 107, 141, 503, 509, 515, 2289, 2653, 2722
FauI CCCGC 1 cut(s) 3411
FbaI TGATCA 2 cut(s) 940, 3016
Fnu4HI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
FriOI GRGCYC 2 cut(s) 1121, 2830
Fsp4HI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
FspBI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
GlaI GCGC 1 cut(s) 3506
GluI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
GsaI CCCAGC 1 cut(s) 2598
HapII CCGG 5 cut(s) 1099, 1124, 2145, 2246, 3368
HgaI GACGC 2 cut(s) 3500, 3554
HhaI GCGC 1 cut(s) 3507
Hin1I GRCGYC 1 cut(s) 3565
Hin6I GCGC 1 cut(s) 3505
HinP1I GCGC 1 cut(s) 3505
HincII GTYRAC 1 cut(s) 3436
HindII GTYRAC 1 cut(s) 3436
HinfI GANTC 9 cut(s) 263, 992, 1333, 1351, 1430, 1450, 1939, 2546, 2965
HpaII CCGG 5 cut(s) 1099, 1124, 2145, 2246, 3368
HphI GGTGA 9 cut(s) 874, 904, 1113, 1305, 1496, 1706, 2264, 2772, 3436
Hpy166II GTNNAC 6 cut(s) 209, 1776, 1778, 2923, 3436, 3575
Hpy8I GTNNAC 6 cut(s) 209, 1776, 1778, 2923, 3436, 3575
Hpy99I CGWCG 1 cut(s) 1015
HpyCH4IV ACGT 3 cut(s) 1786, 3207, 3520
HpyF10VI GCNNNNNNNGC 5 cut(s) 1408, 1624, 2600, 3089, 3254
HpySE526I ACGT 3 cut(s) 1786, 3207, 3520
Hsp92I GRCGYC 1 cut(s) 3565
HspAI GCGC 1 cut(s) 3505
KroI GCCGGC 1 cut(s) 1098
KroNI GCCGGC 1 cut(s) 1100
Ksp22I TGATCA 2 cut(s) 940, 3016
LmnI GCTCC 4 cut(s) 1229, 1387, 2242, 2825
Lsp1109I GCAGC 7 cut(s) 1348, 1370, 1420, 1423, 1783, 2194, 3262
MabI ACCWGGT 1 cut(s) 1292
MaeI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
MaeII ACGT 3 cut(s) 1786, 3207, 3520
MflI RGATCY 5 cut(s) 1444, 1465, 2770, 2887, 2943
MhlI GDGCHC 6 cut(s) 281, 1121, 2377, 2830, 3157, 3352
MlsI TGGCCA 1 cut(s) 2915
MluNI TGGCCA 1 cut(s) 2915
MlyI GAGTC 2 cut(s) 257, 1360
MmeI TCCRAC 3 cut(s) 348, 1407, 1837
Mox20I TGGCCA 1 cut(s) 2915
Mph1103I ATGCAT 1 cut(s) 2209
MroNI GCCGGC 1 cut(s) 1098
MroXI GAANNNNTTC 1 cut(s) 1653
MscI TGGCCA 1 cut(s) 2915
MseI TTAA 7 cut(s) 888, 1257, 1329, 1383, 2213, 2237, 2720
MslI CAYNNNNRTG 7 cut(s) 570, 576, 690, 708, 1757, 2013, 2960
Msp20I TGGCCA 1 cut(s) 2915
MspA1I CMGCKG 1 cut(s) 1361
MspI CCGG 5 cut(s) 1099, 1124, 2145, 2246, 3368
MspR9I CCNGG 9 cut(s) 1294, 1721, 2145, 2421, 2502, 2619, 3011, 3466, 3562
MvaI CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
MwoI GCNNNNNNNGC 5 cut(s) 1408, 1624, 2600, 3089, 3254
NaeI GCCGGC 1 cut(s) 1100
NciI CCSGG 1 cut(s) 2145
NgoMIV GCCGGC 1 cut(s) 1098
NlaIV GGNNCC 7 cut(s) 181, 2244, 2772, 2827, 2889, 3108, 3288
NmeAIII GCCGAG 5 cut(s) 208, 814, 2122, 2482, 2899
NmuCI GTSAC 7 cut(s) 1036, 1311, 1573, 2074, 2252, 2304, 3424
NsiI ATGCAT 1 cut(s) 2209
NspI RCATGY 2 cut(s) 1540, 2301
PaeI GCATGC 1 cut(s) 2301
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 571, 1993
PceI AGGCCT 1 cut(s) 2992
PcsI WCGNNNNNNNCGW 1 cut(s) 2596
PdiI GCCGGC 1 cut(s) 1100
PdmI GAANNNNTTC 1 cut(s) 1653
PfeI GAWTC 7 cut(s) 992, 1333, 1430, 1450, 1939, 2546, 2965
PflMI CCANNNNNTGG 2 cut(s) 1481, 3391
PkrI GCNGC 8 cut(s) 7, 1360, 1363, 1410, 1413, 1798, 2184, 3277
PleI GAGTC 2 cut(s) 257, 1359
PpsI GAGTC 2 cut(s) 257, 1359
Ppu21I YACGTR 1 cut(s) 1787
PpuMI RGGWCCY 1 cut(s) 3286
PsiI TTATAA 2 cut(s) 735, 2526
Psp124BI GAGCTC 1 cut(s) 1121
Psp5II RGGWCCY 1 cut(s) 3286
Psp6I CCWGG 8 cut(s) 1292, 1719, 2419, 2500, 2617, 3009, 3464, 3560
PspFI CCCAGC 1 cut(s) 2594
PspGI CCWGG 8 cut(s) 1292, 1719, 2419, 2500, 2617, 3009, 3464, 3560
PspN4I GGNNCC 7 cut(s) 181, 2244, 2772, 2827, 2889, 3108, 3288
PspPI GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
PspPPI RGGWCCY 1 cut(s) 3286
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 1 cut(s) 3259
PstNI CAGNNNCTG 3 cut(s) 1481, 1745, 2618
PsuI RGATCY 5 cut(s) 1444, 1465, 2770, 2887, 2943
PvuII CAGCTG 1 cut(s) 1361
RsaI GTAC 6 cut(s) 38, 208, 1777, 1910, 1967, 2611
RsaNI GTAC 6 cut(s) 37, 207, 1776, 1909, 1966, 2610
RseI CAYNNNNRTG 7 cut(s) 570, 576, 690, 708, 1757, 2013, 2960
SacI GAGCTC 1 cut(s) 1121
SaqAI TTAA 7 cut(s) 888, 1257, 1329, 1383, 2213, 2237, 2720
SatI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
Sau96I GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
ScaI AGTACT 1 cut(s) 1967
SchI GAGTC 2 cut(s) 257, 1360
ScrFI CCNGG 9 cut(s) 1294, 1721, 2145, 2421, 2502, 2619, 3011, 3466, 3562
SduI GDGCHC 6 cut(s) 281, 1121, 2377, 2830, 3157, 3352
SexAI ACCWGGT 1 cut(s) 1292
SfcI CTRYAG 3 cut(s) 1806, 2160, 3255
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 3 cut(s) 1889, 2923, 3286
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 7 cut(s) 570, 576, 690, 708, 1757, 2013, 2960
SmlI CTYRAG 2 cut(s) 32, 1244
SmoI CTYRAG 2 cut(s) 32, 1244
SpeI ACTAGT 1 cut(s) 2551
SphI GCATGC 1 cut(s) 2301
SseBI AGGCCT 1 cut(s) 2992
SsiI CCGC 7 cut(s) 553, 1302, 2050, 2632, 2656, 3418, 3530
SspI AATATT 1 cut(s) 1326
SspMI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
SstI GAGCTC 1 cut(s) 1121
StuI AGGCCT 1 cut(s) 2992
StyD4I CCNGG 9 cut(s) 1292, 1719, 2143, 2419, 2500, 2617, 3009, 3464, 3560
StyI CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
TaiI ACGT 3 cut(s) 1789, 3210, 3523
TaqI TCGA 9 cut(s) 33, 156, 481, 601, 1013, 2121, 2396, 2475, 3139
TatI WGTACW 3 cut(s) 1775, 1908, 1965
TfiI GAWTC 7 cut(s) 992, 1333, 1430, 1450, 1939, 2546, 2965
Tru1I TTAA 7 cut(s) 888, 1257, 1329, 1383, 2213, 2237, 2720
Tru9I TTAA 7 cut(s) 888, 1257, 1329, 1383, 2213, 2237, 2720
TscAI CASTG 9 cut(s) 286, 1668, 1891, 2224, 2389, 2472, 2647, 2941, 3352
TseFI GTSAC 7 cut(s) 1036, 1311, 1573, 2074, 2252, 2304, 3424
TseI GCWGC 8 cut(s) 5, 1358, 1361, 1408, 1411, 1796, 2182, 3275
Tsp45I GTSAC 7 cut(s) 1036, 1311, 1573, 2074, 2252, 2304, 3424
TspGWI ACGGA 2 cut(s) 194, 2255
TspRI CASTG 9 cut(s) 286, 1668, 1891, 2224, 2389, 2472, 2647, 2941, 3352
Van91I CCANNNNNTGG 2 cut(s) 1481, 3391
VpaK11BI GGWCC 3 cut(s) 1889, 2923, 3286
XapI RAATTY 4 cut(s) 152, 2229, 2688, 3226
XceI RCATGY 2 cut(s) 1540, 2301
XcmI CCANNNNNNNNNTGG 1 cut(s) 3448
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 2993
XmnI GAANNNNTTC 1 cut(s) 1653
XspI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
ZrmI AGTACT 1 cut(s) 1967
Zsp2I ATGCAT 1 cut(s) 2209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.