RLG00000009162

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000671
Physical Location & Seq
Forward (+)
47808050 .. 47810347
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009162

Sequence Viewer

Length: 2298 bp
ATGGCCTTGTCTGAGAAAGACAGTGTTTTCGACAAGCTTGCATACCTTTCTCTGGTCTCCAGGATTTGCATAGAGCTTGAAAATCATGATGGGGGTTTTGGAGACAAAGTCTTTGCAGAGTTGATAGCCGACATGGGACGAAACTGTGAGGCAGTGGACGAGTTCCATAGAGAGTTGAAGCAAAACGGTATTGACATGCCTGAAAATGTCGTGTCTATGTTGTTAACAATTATTCACTCTGTTCTTCCTCCGACAAAGGTATCTGAGAAGAACAAAATCCCAGACATGGCAATTAACAATAGCATGCAAAGGCATCGAGATGATAAACGAGGTAAAAGTGAGCTTAGGCGTAGAAGGAGGCATAGAAATTCACAGCAGAATAGGAACTTGCCAATATACAAGTTGAAGAATGAGCTTATTCAAGCTGTGCATGAAAATCAGGTTCTTGTTGTCATCGGTGAAACTGGTTCGGGCAAGACAACTCAGGTAACACAGTATCTTGCTGAAGCAGGTTACACCACAAAGGGCAAGATTGGATGTACTCAACCCCGTAGAGTTGCGGTGACGTCTGTGGCCAAGAGGGTTGCTGAAGAATTTGGTTGTGGATTAGGGGAGGAAGTGGGGTATGCTATTCGTTTTGAGGATTGTACTGGACCAGAAACTGTGATCAAGTACATGACTGATGGGATGCTCTTTAGGGAGATTTTGGTTGATAATGATCTTTCTCAGTACTCTGTGATCATGCTTGATGAAGCTCATGAAAGAACCATCTACACTGATGTCCTCTTCGGATTGCTGAAGCAACTTGTGAAACGACGTCCTGATCTTCGCTTGATTGTCACCTCTGCCACATTGGATGCTGAGAAGTTTTCGGCTTATTTCTTCTACTGTAAGATATTTACTGTTCCTGGTAGAACTTATCCTGTAGAATTGTTCTACTTGAATCAGCCCGAAAGGGATTACTTGGATTCAGCTCTATTAACTGTTGTGCAGATTCACTTGACAGAACCTGAGGGTGACATACTTCTCTTCTTGACTGGCGAGGAGGAGATTGAATCTGCTTGTCAGTCTCTGAATGAAAGGATGAAAGAATATGGAAAAGGCGTTCCAGAATTGATCATTCTACCAGCTTATGGTGCCCAACCTAGTGAAGAACAGGCGAAGATATTTAAGCCTACTCCTCCAGGGAAGAGGAAGGTGGTGGTGGCTACCAACATTGCTGAAGCTTCTTTAACCATTGATGGGATATTTTATGTCATTGATTCAGGGTATGCAAAGCAAAATGTGTATAATCCGAAGAAAGGGATTGATCTGCTGACTGTAACTCCAATTTCACAAGCATCAGCCAAGCAACGAGCTGGACGCGCTGGGCGGACAGGACCAGGCAACTGTTACCGCCTTTACACTGAGGCTGCATTTAAGAATGAGATGTCTCCTACAACAGTTCCAGAAATCCAGAGAACAAATCTTGTATATACCACACTTATGATGAAAGCAATGGGAATAAACGAGGTTCTATCTTTTGACTTCATGGATCCACCGCCTTCCCAAGCGCTTATTTCTGCTTCGAAACAGCTGCAAAATCTAGGAGCATTGGATGCAGAGGGGTCGGTGACAAAATTGGGTAGTAGAATGGCAGTGTTGCCTCTAGATCCACCTTTGTCGAAAATGCTGCTTGCCAGTGTGAACCTCGGATGCAGTGAGGAGATTTTGACCATCATTGCAATGGTTGAGGCAGGCAATATCTTCTACAGGCCTAGAGGAAAACAAGCTGAAGCTGATCAGAAAAGGGCCAGGTTCTTACAACCTGAGGGAGACCATCTGACTTTACTTGCTGTTTATGAGGCATGGAAAGCTAAGAATTTCTCTGTACCCTGGTGTTCTGAGAACTTTGTCCAGTTTCGGTCACTGAGCAAGGCTCGGGATATCAGGAGACAGCTTCTTACTGCCATGGACAAGCATGAATTAGAGGTTGTAAGTGCAGGGAAAGATCGCACAATGATCAGAAAGGCGATTGTAGCTGGATTCTTCTTCCAAGTAGCTAAAAGGGATCCACACGAGGGTTATAGAACCTTTGTTGATAATCAGCTAGTTTTTATCCACCCAAGCAGTGTTCTTTTCCGGAGACAACCAGATTGGGTCATCTACAATGAGCTGGTGATGACCACAAAGGAGTACATGCGCGAGATCAGTGTTATAGAATCTAAATGGCTCATGGAACTGGTGCCGGGATTTTTTAAGGTAACAATTCCTCCAAGGATGAGCAAGCGGAAACGTCAAGAAATTGAACCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

766

Amino Acids

86.51

Weight (kDa)

8.12

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 137 - 286 9.2e-08 DEAD/DEAH box helicase
AAA_22 PF13401 145 - 278 5.9e-06 AAA domain
Helicase_C PF00271 330 - 460 1.7e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 524 - 550 5.3e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 551 - 610 4.1e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 668 - 743 1.2e-25 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 569, 820
Acc36I ACCTGC 1 cut(s) 500
AccB1I GGYRCC 2 cut(s) 1136, 2224
AccB7I CCANNNNNTGG 1 cut(s) 1133
AccII CGCG 2 cut(s) 1365, 2184
AccIII TCCGGA 1 cut(s) 2121
AciI CCGC 5 cut(s) 560, 1372, 1396, 1541, 2269
AclWI GGATC 5 cut(s) 1529, 1542, 1646, 2045, 2058
AcoI YGGCCR 1 cut(s) 573
AcsI RAATTY 3 cut(s) 367, 593, 1861
AcuI CTGAAG 5 cut(s) 525, 609, 818, 1242, 1794
AcyI GRCGYC 2 cut(s) 566, 817
AfaI GTAC 6 cut(s) 541, 649, 674, 731, 1872, 2177
AfeI AGCGCT 1 cut(s) 1554
AfiI CCNNNNNNNGG 6 cut(s) 52, 286, 1133, 1242, 1301, 2060
AgsI TTSAA 7 cut(s) 80, 178, 406, 422, 943, 1055, 2288
AjnI CCWGG 6 cut(s) 59, 907, 1183, 1381, 1793, 1874
AloI GAACNNNNNNTCC 2 cut(s) 1089, 1121
Alw26I GTCTC 7 cut(s) 61, 96, 1074, 1437, 1809, 1927, 2119
AlwI GGATC 5 cut(s) 1529, 1542, 1646, 2045, 2058
AlwNI CAGNNNCTG 3 cut(s) 662, 1010, 1072
Ama87I CYCGRG 1 cut(s) 1920
Aor13HI TCCGGA 1 cut(s) 2121
Aor51HI AGCGCT 1 cut(s) 1554
AoxI GGCC 4 cut(s) 3, 573, 1754, 1791
ApeKI GCWGC 3 cut(s) 1412, 1576, 1672
ApoI RAATTY 3 cut(s) 367, 593, 1861
ArsI GACNNNNNNTTYG 4 cut(s) 11, 43, 95, 127
AspLEI GCGC 3 cut(s) 1367, 1555, 2184
AspS9I GGNCC 3 cut(s) 653, 1379, 1791
AsuC2I CCSGG 1 cut(s) 2229
AsuHPI GGTGA 6 cut(s) 470, 574, 832, 1028, 1624, 2170
AsuII TTCGAA 1 cut(s) 1568
AvaI CYCGRG 1 cut(s) 1920
AvaII GGWCC 2 cut(s) 653, 1379
AxyI CCTNAGG 2 cut(s) 1011, 1809
BaeGI GKGCMC 1 cut(s) 1141
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BalI TGGCCA 1 cut(s) 575
BamHI GGATCC 2 cut(s) 1534, 2050
BanI GGYRCC 2 cut(s) 1136, 2224
BauI CACGAG 1 cut(s) 2057
BbvI GCAGC 3 cut(s) 1399, 1563, 1659
BccI CCATC 6 cut(s) 83, 677, 776, 1235, 1724, 1827
BciT130I CCWGG 6 cut(s) 61, 909, 1185, 1383, 1795, 1876
BclI TGATCA 5 cut(s) 666, 738, 1116, 1780, 2001
BcnI CCSGG 1 cut(s) 2229
BcoDI GTCTC 7 cut(s) 61, 96, 1074, 1437, 1809, 1927, 2119
BfaI CTAG 6 cut(s) 1146, 1586, 1649, 1758, 2090, 2296
BfmI CTRYAG 2 cut(s) 924, 1750
BfoI RGCGCY 1 cut(s) 1556
BfuAI ACCTGC 1 cut(s) 500
BisI GCNGC 3 cut(s) 1413, 1577, 1673
BlsI GCNGC 3 cut(s) 1414, 1578, 1674
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 7 cut(s) 61, 909, 1185, 1383, 1795, 1876, 2229
Bme18I GGWCC 2 cut(s) 653, 1379
BmeT110I CYCGRG 1 cut(s) 1920
BmgT120I GGNCC 3 cut(s) 653, 1379, 1791
BmiI GGNNCC 4 cut(s) 1138, 1536, 2052, 2226
BmrFI CCNGG 7 cut(s) 61, 909, 1185, 1383, 1795, 1876, 2229
BmsI GCATC 6 cut(s) 322, 678, 847, 1349, 1588, 1685
BplI GAGNNNNNCTC 2 cut(s) 1903, 1935
BpmI CTGGAG 2 cut(s) 43, 1167
Bpu10I CCTNAGC 1 cut(s) 344
Bpu14I TTCGAA 1 cut(s) 1568
BpuMI CCSGG 1 cut(s) 2229
BsaBI GATNNNNATC 1 cut(s) 717
BsaHI GRCGYC 2 cut(s) 566, 817
BsaI GGTCTC 2 cut(s) 61, 1809
BsaJI CCNNGG 5 cut(s) 1184, 1690, 1874, 1950, 2255
BsaWI WCCGGW 1 cut(s) 2121
BsaXI ACNNNNNCTCC 6 cut(s) 93, 123, 1309, 1339, 2236, 2266
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 286, 1133, 1242, 1301, 2060
Bse1I ACTGG 6 cut(s) 469, 655, 1042, 1680, 1897, 2226
Bse21I CCTNAGG 2 cut(s) 1011, 1809
Bse3DI GCAATG 4 cut(s) 1215, 1503, 1719, 1731
Bse8I GATNNNNATC 1 cut(s) 717
BseAI TCCGGA 1 cut(s) 2121
BseBI CCWGG 6 cut(s) 61, 909, 1185, 1383, 1795, 1876
BseDI CCNNGG 5 cut(s) 1184, 1690, 1874, 1950, 2255
BseGI GGATG 7 cut(s) 542, 693, 862, 1089, 1603, 1700, 2265
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 6 cut(s) 52, 286, 1133, 1242, 1301, 2060
BseMI GCAATG 4 cut(s) 1215, 1503, 1719, 1731
BseMII CTCAG 9 cut(s) 255, 497, 740, 852, 1002, 1398, 1800, 1875, 1901
BseNI ACTGG 6 cut(s) 469, 655, 1042, 1680, 1897, 2226
BseRI GAGGAG 4 cut(s) 1058, 1061, 1170, 1718
BseSI GKGCMC 1 cut(s) 1141
BseXI GCAGC 3 cut(s) 1399, 1563, 1659
BseYI CCCAGC 1 cut(s) 1367
BsgI GTGCAG 2 cut(s) 1010, 2001
Bsh1236I CGCG 2 cut(s) 1365, 2184
BshFI GGCC 4 cut(s) 5, 575, 1756, 1793
BshNI GGYRCC 2 cut(s) 1136, 2224
BsiHKCI CYCGRG 1 cut(s) 1920
BsiSI CCGG 2 cut(s) 2122, 2228
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 6 cut(s) 52, 286, 1133, 1242, 1301, 2060
BsmAI GTCTC 7 cut(s) 61, 96, 1074, 1437, 1809, 1927, 2119
BsmFI GGGAC 1 cut(s) 150
BsnI GGCC 4 cut(s) 5, 575, 1756, 1793
Bso31I GGTCTC 2 cut(s) 61, 1809
BsoBI CYCGRG 1 cut(s) 1920
Bsp119I TTCGAA 1 cut(s) 1568
Bsp1286I GDGCHC 1 cut(s) 1141
Bsp13I TCCGGA 1 cut(s) 2121
Bsp19I CCATGG 1 cut(s) 1950
BspACI CCGC 5 cut(s) 560, 1372, 1396, 1541, 2269
BspANI GGCC 4 cut(s) 5, 575, 1756, 1793
BspEI TCCGGA 1 cut(s) 2121
BspFNI CGCG 2 cut(s) 1365, 2184
BspHI TCATGA 2 cut(s) 85, 757
BspLI GGNNCC 4 cut(s) 1138, 1536, 2052, 2226
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 5 cut(s) 1529, 1542, 1646, 2045, 2058
BspT104I TTCGAA 1 cut(s) 1568
BspT107I GGYRCC 2 cut(s) 1136, 2224
BspTNI GGTCTC 2 cut(s) 61, 1809
BsrDI GCAATG 4 cut(s) 1215, 1503, 1719, 1731
BsrI ACTGG 6 cut(s) 469, 655, 1042, 1680, 1897, 2226
BssECI CCNNGG 5 cut(s) 1184, 1690, 1874, 1950, 2255
BssNI GRCGYC 2 cut(s) 566, 817
BssSI CACGAG 1 cut(s) 2057
BssT1I CCWWGG 2 cut(s) 1950, 2255
Bst2BI CACGAG 1 cut(s) 2057
Bst2UI CCWGG 6 cut(s) 61, 909, 1185, 1383, 1795, 1876
Bst6I CTCTTC 3 cut(s) 791, 1034, 1184
BstACI GRCGYC 2 cut(s) 566, 817
BstAPI GCANNNNNTGC 1 cut(s) 1598
BstBI TTCGAA 1 cut(s) 1568
BstC8I GCNNGC 5 cut(s) 39, 305, 1677, 1738, 2267
BstDSI CCRYGG 1 cut(s) 1950
BstF5I GGATG 7 cut(s) 542, 693, 862, 1089, 1603, 1700, 2265
BstFNI CGCG 2 cut(s) 1365, 2184
BstH2I RGCGCY 1 cut(s) 1556
BstHHI GCGC 3 cut(s) 1367, 1555, 2184
BstMAI GTCTC 7 cut(s) 61, 96, 1074, 1437, 1809, 1927, 2119
BstMWI GCNNNNNNNGC 5 cut(s) 1136, 1364, 1598, 1853, 2018
BstNI CCWGG 6 cut(s) 61, 909, 1185, 1383, 1795, 1876
BstNSI RCATGY 3 cut(s) 199, 307, 2182
BstSCI CCNGG 7 cut(s) 59, 907, 1183, 1381, 1793, 1874, 2227
BstSFI CTRYAG 2 cut(s) 924, 1750
BstSLI GKGCMC 1 cut(s) 1141
BstUI CGCG 2 cut(s) 1365, 2184
BstV1I GCAGC 3 cut(s) 1399, 1563, 1659
BstX2I RGATCY 3 cut(s) 1534, 1651, 2050
BstYI RGATCY 3 cut(s) 1534, 1651, 2050
Bsu36I CCTNAGG 2 cut(s) 1011, 1809
BsuRI GGCC 4 cut(s) 5, 575, 1756, 1793
BtgI CCRYGG 1 cut(s) 1950
BtsCI GGATG 7 cut(s) 542, 693, 862, 1089, 1603, 1700, 2265
BtsI GCAGTG 4 cut(s) 159, 1644, 1705, 2116
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 5 cut(s) 39, 305, 1677, 1738, 2267
CaiI CAGNNNCTG 3 cut(s) 662, 1010, 1072
CciI TCATGA 2 cut(s) 85, 757
CfoI GCGC 3 cut(s) 1367, 1555, 2184
Cfr13I GGNCC 3 cut(s) 653, 1379, 1791
CseI GACGC 1 cut(s) 1371
Csp6I GTAC 6 cut(s) 540, 648, 673, 730, 1871, 2176
CviQI GTAC 6 cut(s) 540, 648, 673, 730, 1871, 2176
EaeI YGGCCR 1 cut(s) 573
Eam1104I CTCTTC 3 cut(s) 791, 1034, 1184
EarI CTCTTC 3 cut(s) 791, 1034, 1184
EciI GGCGGA 1 cut(s) 1387
Eco130I CCWWGG 2 cut(s) 1950, 2255
Eco147I AGGCCT 1 cut(s) 1756
Eco31I GGTCTC 2 cut(s) 61, 1809
Eco32I GATATC 1 cut(s) 1927
Eco47I GGWCC 2 cut(s) 653, 1379
Eco47III AGCGCT 1 cut(s) 1554
Eco57I CTGAAG 5 cut(s) 525, 609, 818, 1242, 1794
Eco81I CCTNAGG 2 cut(s) 1011, 1809
Eco88I CYCGRG 1 cut(s) 1920
EcoRII CCWGG 6 cut(s) 59, 907, 1183, 1381, 1793, 1874
EcoRV GATATC 1 cut(s) 1927
EcoT14I CCWWGG 2 cut(s) 1950, 2255
ErhI CCWWGG 2 cut(s) 1950, 2255
FaqI GGGAC 1 cut(s) 150
FbaI TGATCA 5 cut(s) 666, 738, 1116, 1780, 2001
Fnu4HI GCNGC 3 cut(s) 1413, 1577, 1673
FokI GGATG 7 cut(s) 549, 700, 869, 1096, 1610, 1707, 2272
Fsp4HI GCNGC 3 cut(s) 1413, 1577, 1673
FspBI CTAG 6 cut(s) 1146, 1586, 1649, 1758, 2090, 2296
GlaI GCGC 3 cut(s) 1366, 1554, 2183
GluI GCNGC 3 cut(s) 1413, 1577, 1673
GsaI CCCAGC 1 cut(s) 1371
GsuI CTGGAG 2 cut(s) 43, 1167
HaeII RGCGCY 1 cut(s) 1556
HaeIII GGCC 4 cut(s) 5, 575, 1756, 1793
HapII CCGG 2 cut(s) 2122, 2228
HgaI GACGC 1 cut(s) 1371
HhaI GCGC 3 cut(s) 1367, 1555, 2184
Hin1I GRCGYC 2 cut(s) 566, 817
Hin6I GCGC 3 cut(s) 1365, 1553, 2182
HinP1I GCGC 3 cut(s) 1365, 1553, 2182
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HindIII AAGCTT 2 cut(s) 35, 1224
HinfI GANTC 7 cut(s) 943, 968, 994, 1055, 1262, 2025, 2201
HpaI GTTAAC 1 cut(s) 225
HpaII CCGG 2 cut(s) 2122, 2228
HphI GGTGA 6 cut(s) 470, 574, 832, 1028, 1624, 2170
Hpy166II GTNNAC 3 cut(s) 157, 225, 1687
Hpy8I GTNNAC 3 cut(s) 157, 225, 1687
Hpy99I CGWCG 1 cut(s) 819
HpyAV CCTTC 3 cut(s) 348, 1189, 1554
HpyCH4IV ACGT 3 cut(s) 566, 817, 2275
HpyF10VI GCNNNNNNNGC 5 cut(s) 1136, 1364, 1598, 1853, 2018
HpySE526I ACGT 3 cut(s) 566, 817, 2275
Hsp92I GRCGYC 2 cut(s) 566, 817
HspAI GCGC 3 cut(s) 1365, 1553, 2182
Kpn2I TCCGGA 1 cut(s) 2121
Ksp22I TGATCA 5 cut(s) 666, 738, 1116, 1780, 2001
KspAI GTTAAC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 1589
Lsp1109I GCAGC 3 cut(s) 1399, 1563, 1659
LweI GCATC 6 cut(s) 322, 678, 847, 1349, 1588, 1685
MaeI CTAG 6 cut(s) 1146, 1586, 1649, 1758, 2090, 2296
MaeII ACGT 3 cut(s) 566, 817, 2275
MflI RGATCY 3 cut(s) 1534, 1651, 2050
MhlI GDGCHC 1 cut(s) 1141
MlsI TGGCCA 1 cut(s) 575
MluNI TGGCCA 1 cut(s) 575
MmeI TCCRAC 1 cut(s) 275
Mox20I TGGCCA 1 cut(s) 575
MroI TCCGGA 1 cut(s) 2121
MscI TGGCCA 1 cut(s) 575
MseI TTAA 7 cut(s) 224, 294, 980, 1170, 1232, 1419, 2238
MslI CAYNNNNRTG 3 cut(s) 318, 1484, 1724
Msp20I TGGCCA 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 1576
MspI CCGG 2 cut(s) 2122, 2228
MspR9I CCNGG 7 cut(s) 61, 909, 1185, 1383, 1795, 1876, 2229
MvaI CCWGG 6 cut(s) 61, 909, 1185, 1383, 1795, 1876
MvnI CGCG 2 cut(s) 1365, 2184
MwoI GCNNNNNNNGC 5 cut(s) 1136, 1364, 1598, 1853, 2018
NciI CCSGG 1 cut(s) 2229
NcoI CCATGG 1 cut(s) 1950
NlaIV GGNNCC 4 cut(s) 1138, 1536, 2052, 2226
NmuCI GTSAC 5 cut(s) 562, 838, 1016, 1612, 1905
NspI RCATGY 3 cut(s) 199, 307, 2182
NspV TTCGAA 1 cut(s) 1568
PaeI GCATGC 1 cut(s) 307
PagI TCATGA 2 cut(s) 85, 757
PceI AGGCCT 1 cut(s) 1756
PfeI GAWTC 7 cut(s) 943, 968, 994, 1055, 1262, 2025, 2201
PflFI GACNNNGTC 1 cut(s) 107
PflMI CCANNNNNTGG 1 cut(s) 1133
PfoI TCCNGGA 1 cut(s) 59
PkrI GCNGC 3 cut(s) 1414, 1578, 1674
Psp6I CCWGG 6 cut(s) 59, 907, 1183, 1381, 1793, 1874
PspFI CCCAGC 1 cut(s) 1367
PspGI CCWGG 6 cut(s) 59, 907, 1183, 1381, 1793, 1874
PspN4I GGNNCC 4 cut(s) 1138, 1536, 2052, 2226
PspPI GGNCC 3 cut(s) 653, 1379, 1791
PstNI CAGNNNCTG 3 cut(s) 662, 1010, 1072
PsuI RGATCY 3 cut(s) 1534, 1651, 2050
PsyI GACNNNGTC 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 1576
RsaI GTAC 6 cut(s) 541, 649, 674, 731, 1872, 2177
RsaNI GTAC 6 cut(s) 540, 648, 673, 730, 1871, 2176
RseI CAYNNNNRTG 3 cut(s) 318, 1484, 1724
SaqAI TTAA 7 cut(s) 224, 294, 980, 1170, 1232, 1419, 2238
SatI GCNGC 3 cut(s) 1413, 1577, 1673
Sau96I GGNCC 3 cut(s) 653, 1379, 1791
ScaI AGTACT 1 cut(s) 731
ScrFI CCNGG 7 cut(s) 61, 909, 1185, 1383, 1795, 1876, 2229
SduI GDGCHC 1 cut(s) 1141
SfaNI GCATC 6 cut(s) 322, 678, 847, 1349, 1588, 1685
SfcI CTRYAG 2 cut(s) 924, 1750
SfuI TTCGAA 1 cut(s) 1568
SinI GGWCC 2 cut(s) 653, 1379
SmiMI CAYNNNNRTG 3 cut(s) 318, 1484, 1724
SphI GCATGC 1 cut(s) 307
SseBI AGGCCT 1 cut(s) 1756
SsiI CCGC 5 cut(s) 560, 1372, 1396, 1541, 2269
SspMI CTAG 6 cut(s) 1146, 1586, 1649, 1758, 2090, 2296
StuI AGGCCT 1 cut(s) 1756
StyD4I CCNGG 7 cut(s) 59, 907, 1183, 1381, 1793, 1874, 2227
StyI CCWWGG 2 cut(s) 1950, 2255
TaiI ACGT 3 cut(s) 569, 820, 2278
TaqI TCGA 4 cut(s) 30, 316, 1568, 1664
TaqII GACCGA 1 cut(s) 1893
TatI WGTACW 5 cut(s) 539, 647, 672, 729, 2175
TfiI GAWTC 7 cut(s) 943, 968, 994, 1055, 1262, 2025, 2201
Tru1I TTAA 7 cut(s) 224, 294, 980, 1170, 1232, 1419, 2238
Tru9I TTAA 7 cut(s) 224, 294, 980, 1170, 1232, 1419, 2238
TseFI GTSAC 5 cut(s) 562, 838, 1016, 1612, 1905
TseI GCWGC 3 cut(s) 1412, 1576, 1672
Tsp45I GTSAC 5 cut(s) 562, 838, 1016, 1612, 1905
TspDTI ATGAA 8 cut(s) 447, 765, 774, 1092, 1100, 1505, 1519, 1977
Tth111I GACNNNGTC 1 cut(s) 107
Van91I CCANNNNNTGG 1 cut(s) 1133
VpaK11BI GGWCC 2 cut(s) 653, 1379
XapI RAATTY 3 cut(s) 367, 593, 1861
XbaI TCTAGA 1 cut(s) 1648
XceI RCATGY 3 cut(s) 199, 307, 2182
XcmI CCANNNNNNNNNTGG 1 cut(s) 1723
XspI CTAG 6 cut(s) 1146, 1586, 1649, 1758, 2090, 2296
ZraI GACGTC 2 cut(s) 567, 818
ZrmI AGTACT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.