Rorug01G0221400

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000671
Physical Location & Seq
Reverse (-)
31748229 .. 31752178
3950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0221400.1

Sequence Viewer

Length: 3375 bp
ATGGAGAGTTTCACATACCATCAAGTCCTGACCCTTCTCCTTTTCATCAACTTTTTACAACCTACCACTGTTTTAAGTTCATTTGGCAATGAAACCGATCGCTTGGCTTTGCTAAAATTCAAAGATTGCATAGCCTCTGATCCACATGGGCTGTTGAACTCATGGAATGACTCCGTTCATTATTGCAAATGGCTTGGAATTACTTGTGGCAGACGACATCAAAGAGTAACAGCCTTGAACCTACCACACGCTGTTTTGCACGGAACCATATCTCCTTACATTGGCAACCTCTCCTTTCTTAGGTTCATCAACCTTCGAAACAACAGCTTCTCTGGCAATATTCCGCAACAAGTTGAACATTTATTCCGACTCCGACATCTCAATCTCAGTATCAACATGTTGGAGGGGGGAATTCCAGTCAACCTGACCTTCTCCCGCCAATTAAGCATCATAACCATTGCATGGAACCGCCTTACTGGGAAAATTCCTTCAGAGATCGGTTCACTGAAGCTTGTGTGTCTTGATCTTCAGATTAACAATCTGACAGGAGGCATACCACCTTCCTTGGGAAATCTTTCATCAATCACTTTTCTTTCCTTACAAGAGAACAATTTGGTGGGCAACGTACCAGAGGAAATAGGCCGATTAAGAAGCTTATCATTTTTTTCAATTGGTCCCAATAATCTCTCGGGTACGATACCTCCCTCCTTTTTTAACATATCATCTATGAACCGCTTCTCACTTTCAGCTAATAAATTTAAGGGCAGTATTCCACCTGGTATAGGCCTAAACATGCCTAATCTCCAAGAAGTGTACCTTGGTACAAATGAATTCTCTGGCCAAATCCCAGCTTCATTTTCCAATGCTTCTCAGCTTCAGGTACTTGATGTTGGGGAAAATAATTTTGTTGGGCAAGTTCCCGCAAGTTTTGGAAATTTTCCCAATCTCCAGCTGCTCAACTTCGAGGTCAATAATCTAGGAACTAATTCATCAAATGATTTGGGATTTATAACATTCTTGACAAATTGCAGCAATTTGTCGCTATTTTCTCTGAGTAATAACAATTTTGGAGGTGTTTTACCCAATTCTGTAGCCAATTTCTCAACCCAACTGACTCAACTCTGCCTTGGGGCCAATCAAATAGCGGGAACGATTCCTGAAACATTAGAAAATCTCAGCAATTTAATACTCCTGACCCTGGAAGAAAACTTGTTCACAGGTACCATTCCAGCTTCTTTTGGGAAGTTACAAAAGCTGCAAGTATTAAGTTTAGATTCCAATAGATTATCAGGCCAGATCCCATCTTCCTTAGGAAACCTCACCCAATTATATCACCTCCACTTATTAGAAAATGAATTAGAAGGAAGCATTCCTCCAAATATTGGTAACTGCAAAAATCTGCAGCAGATGGATATATCAGACAATAAGCTTAGTGGAGATATACCATCACAGGTGATTGGTATGTCCTTCTCTGTCTTGCTCAACTTATCACAAAACTCGCTGACTGGCATTCTGCCTGTGGAAGTGGGTAAGATGAAGAATATCAATACACTGGACATCTCTGATAATAATTTGACCGGAGGAATTCCAGAAATTATTGGAGGCTGTCTGAGCCTTGAATTTCTTTACCTACAAGGGAACCACTTTCAAGGAATCGTACCTTCTTCTTTAGCTGCTTTGAGAGGTCTTCAGTATCTAGATCTTTCACGAAACAACTTGTCAGGACATATTCCAAAAGACCTACAGAGGCTTCCATTCTTGATCCATTTGAACCTTTCGTTCAATAATCTGGAGGGTGAGGTACCAAAAGAAAGAGTTTTTCAAAACACAAGTGCAATATCATTGGATGGAAATACCAAACTTTGTGGTGGTGTTTCAGAATTGCAGCTACCAGCATGCCCCATCAAAGTACCAAAGCAGAGAAAGTTGCATGCTTTCAAACTAAAGTTCACAATTTCCTTGGTCGCTGGATGCTCTCTTTTGTTTGCAGTCGTCTCAGCTCTTTATTGGAGGAGAAAAACTCAAAAGAAGAAGCCATTAATAGCAGTGTCATCGATTAACTTCCTTCCAAAGGTTTCATACCAGACACTTCATCATGCTACTGGCGGATTCTCTCCGAGCAATCAAATTGGATCAGGAGGTTTTGGCTCTGTATACAAAGGGATTTTTAATCAAGAAGAAAACAACGTTGTTGCCATCAAGGTCCTCAACCTTCAACGGAAAGGTGCTTCCAAGAGTTTTGTGGTTGAATGCAATGCACTGAGAAATATCCGACATAGGAATCTTGTGAAGATCTTAACATGTTGCTCTAGCACAGATTACAATGGTAATGACTTCAAAGCTCTAGTTTTTGAGTATATGTCAAATGGAAATTTAGAGGAGTGGCTGCACAGAGAAAATCAATCAAGGAGTTTGAACCTTCTTCAAAGATTGAATATTGCTGTGGATGTGGCTTCTGCATTGTGTTATCTTCATGACTATTGTGAACCACAAATCATTCACTGCGACATGAAGCCGAGCAACGTTCTTCTTGATGATGACATGGTTGCTCGTGTTGGTGATTTTGGGTTAGCAAGACTCATCTCAACAACCACGGACTCCTCACAAAATCAAAGTAGCACAGTTAGAATAAAGGGAACCATTGGCTATGCTGCTCCAGAGTATGCCTGTGGTGTTGAGCCATCAAGAGAAGGAGATGTATATAGTTATGGGGTGCTCGTGTTGCAAATGTTCACAGGAAGAAGACCTATCGACGATATGTTTAAAGATGGTTTGAACCTCCATAACTTTGTCAAGATGGCCATACCAGAAACAGTGATGCAGATTGTAGATCCTACTCTTCTTGCCACTTTAGAAGAGACAGCACCTGCAACATCACAAAATGTAGTGAACTACATCAAATGTTACAATAATGAAATCGAAGCAGTTGAAGAAAACATTGACAATGAGAATTTAAGCAAGATGAACACTGTGTGGAAGTGCATACTTCCAACCCTTAAGGGCAACTCCAACCTTGGGGTGCCAAACCAGATTTTGGGCCATTTTGAGACTTTGGATCTCCAACCATGTGTTTTTTGGGGGTCTGGTCCTATAAATATAGGACTTGGACTCATTTGTAGTCTCATATTTGAGACTTTTCCAGGACCAGATCCGGAGACTATTCATGAGCTCTATCCATGTGGCTGCATCAGCCCAACTGAAGATGCAACGCGCGCTCCAAGTGAAGTGAAGAAGATGCGCGCTACAAGTGGAAGAAGGAAGCTGACGCGCGCTGAGGCGTTGGAGAGAGAAAAATTCGGCCTTTGGTTACTCTGCCAAAGCGGGGCCCGCGCGAAAATCTTTCCCTTGCCGACCGTTGGGGGTTCTCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1124

Amino Acids

123.41

Weight (kDa)

6.77

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 69 9.2e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 188 - 402 2.4e-09 Leucine-rich repeat region
LRR_8 PF13855 266 - 326 5.4e-06 Leucine rich repeat
LRR_8 PF13855 370 - 429 5.1e-08 Leucine rich repeat
LRR_14 PF23598 388 - 474 5.9e-08 Leucine-rich repeat region
LRR_8 PF13855 494 - 549 3e-06 Leucine rich repeat
LRR_14 PF23598 495 - 599 3.1e-06 Leucine-rich repeat region
LRR_8 PF13855 539 - 597 6.8e-09 Leucine rich repeat
Pkinase PF00069 707 - 932 3.5e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 708 - 922 2e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1010
AarI CACCTGC 1 cut(s) 2875
Acc36I ACCTGC 1 cut(s) 2875
Acc65I GGTACC 2 cut(s) 1220, 1801
AccB1I GGYRCC 3 cut(s) 1220, 1801, 3019
AccB7I CCANNNNNTGG 3 cut(s) 462, 1382, 3034
AccI GTMKAC 1 cut(s) 2154
AccII CGCG 7 cut(s) 3211, 3213, 3240, 3268, 3270, 3330, 3332
AccIII TCCGGA 1 cut(s) 3151
AciI CCGC 9 cut(s) 344, 436, 469, 733, 921, 1145, 2106, 3321, 3328
AclI AACGTT 2 cut(s) 2187, 2523
AclWI GGATC 7 cut(s) 134, 1291, 1756, 2140, 2825, 3063, 3143
AcoI YGGCCR 2 cut(s) 838, 2799
AcuI CTGAAG 6 cut(s) 474, 512, 527, 860, 1673, 3219
AdeI CACNNNGTG 1 cut(s) 2973
AfaI GTAC 9 cut(s) 627, 694, 815, 823, 882, 1222, 1659, 1803, 1911
AflII CTTAAG 1 cut(s) 2996
AflIII ACRYGT 2 cut(s) 396, 2300
AjnI CCWGG 3 cut(s) 775, 1197, 3139
AjuI GAANNNNNNNTTGG 2 cut(s) 801, 833
AloI GAACNNNNNNTCC 4 cut(s) 256, 288, 348, 380
Alw21I GWGCWC 2 cut(s) 2718, 3171
Alw26I GTCTC 6 cut(s) 1999, 2852, 3041, 3125, 3125, 3149
AlwI GGATC 7 cut(s) 134, 1291, 1756, 2140, 2825, 3063, 3143
AlwNI CAGNNNCTG 1 cut(s) 2867
Ama87I CYCGRG 1 cut(s) 688
Aor13HI TCCGGA 1 cut(s) 3151
AoxI GGCC 9 cut(s) 640, 784, 838, 1131, 1291, 2799, 3037, 3298, 3324
ApaI GGGCCC 1 cut(s) 3328
ApeKI GCWGC 9 cut(s) 952, 1029, 1255, 1402, 1673, 1885, 2386, 2651, 3183
AseI ATTAAT 1 cut(s) 2039
Asp700I GAANNNNTTC 3 cut(s) 574, 734, 985
Asp718I GGTACC 2 cut(s) 1220, 1801
AspLEI GCGC 7 cut(s) 3213, 3215, 3240, 3242, 3270, 3272, 3332
AspS9I GGNCC 8 cut(s) 674, 1131, 2203, 3037, 3086, 3143, 3324, 3325
AsuHPI GGTGA 5 cut(s) 1312, 1325, 1465, 1808, 2570
AsuII TTCGAA 1 cut(s) 316
AvaI CYCGRG 1 cut(s) 688
AvaII GGWCC 4 cut(s) 674, 2203, 3086, 3143
AxyI CCTNAGG 1 cut(s) 1309
BaeGI GKGCMC 1 cut(s) 3328
BalI TGGCCA 2 cut(s) 840, 2801
BanI GGYRCC 3 cut(s) 1220, 1801, 3019
BanII GRGCYC 2 cut(s) 3171, 3328
BauI CACGAG 2 cut(s) 2550, 2717
BbsI GAAGAC 2 cut(s) 1679, 2749
Bbv12I GWGCWC 2 cut(s) 2718, 3171
BbvCI CCTCAGC 1 cut(s) 3273
BbvI GCAGC 9 cut(s) 939, 1041, 1242, 1414, 1660, 1897, 2373, 2638, 3170
BcgI CGANNNNNNTGC 2 cut(s) 2034, 2068
BciT130I CCWGG 3 cut(s) 777, 1199, 3141
BcoDI GTCTC 6 cut(s) 1999, 2852, 3041, 3125, 3125, 3149
BfaI CTAG 4 cut(s) 977, 1697, 2310, 2345
BfmI CTRYAG 3 cut(s) 1089, 1400, 1742
BfrI CTTAAG 1 cut(s) 2996
BfuAI ACCTGC 1 cut(s) 2875
BglII AGATCT 2 cut(s) 1699, 2292
BisI GCNGC 9 cut(s) 953, 1030, 1256, 1403, 1674, 1886, 2387, 2652, 3184
BlsI GCNGC 9 cut(s) 954, 1031, 1257, 1404, 1675, 1887, 2388, 2653, 3185
Bme1390I CCNGG 3 cut(s) 777, 1199, 3141
Bme18I GGWCC 4 cut(s) 674, 2203, 3086, 3143
BmeT110I CYCGRG 1 cut(s) 688
BmgT120I GGNCC 8 cut(s) 674, 1131, 2203, 3037, 3086, 3143, 3324, 3325
BmrFI CCNGG 3 cut(s) 777, 1199, 3141
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 6 cut(s) 456, 1961, 2808, 3193, 3195, 3225
BmuI ACTGGG 1 cut(s) 486
BpiI GAAGAC 2 cut(s) 1679, 2749
BplI GAGNNNNNCTC 2 cut(s) 2005, 2037
BpmI CTGGAG 3 cut(s) 932, 1811, 2640
Bpu10I CCTNAGC 1 cut(s) 3273
Bpu14I TTCGAA 1 cut(s) 316
Bsa29I ATCGAT 1 cut(s) 2054
BsaJI CCNNGG 7 cut(s) 564, 817, 1126, 1197, 1959, 2592, 3013
BsaWI WCCGGW 2 cut(s) 1577, 3151
BsaXI ACNNNNNCTCC 6 cut(s) 256, 286, 685, 715, 3199, 3229
Bse1I ACTGG 5 cut(s) 416, 481, 1510, 1557, 2107
Bse21I CCTNAGG 1 cut(s) 1309
Bse3DI GCAATG 3 cut(s) 94, 456, 2260
BseAI TCCGGA 1 cut(s) 3151
BseBI CCWGG 3 cut(s) 777, 1199, 3141
BseCI ATCGAT 1 cut(s) 2054
BseDI CCNNGG 7 cut(s) 564, 817, 1126, 1197, 1959, 2592, 3013
BseGI GGATG 3 cut(s) 1852, 1976, 2452
BseMI GCAATG 3 cut(s) 94, 456, 2260
BseMII CTCAG 8 cut(s) 400, 884, 1043, 1189, 1601, 2010, 2252, 3264
BseNI ACTGG 5 cut(s) 416, 481, 1510, 1557, 2107
BsePI GCGCGC 3 cut(s) 3211, 3238, 3268
BseRI GAGGAG 3 cut(s) 2026, 2393, 2590
BseSI GKGCMC 1 cut(s) 3328
BseXI GCAGC 9 cut(s) 939, 1041, 1242, 1414, 1660, 1897, 2373, 2638, 3170
BseYI CCCAGC 1 cut(s) 847
BsgI GTGCAG 1 cut(s) 2372
Bsh1236I CGCG 7 cut(s) 3211, 3213, 3240, 3268, 3270, 3330, 3332
Bsh1285I CGRYCG 2 cut(s) 100, 3354
BshFI GGCC 9 cut(s) 642, 786, 840, 1133, 1293, 2801, 3039, 3300, 3326
BshNI GGYRCC 3 cut(s) 1220, 1801, 3019
BshVI ATCGAT 1 cut(s) 2054
BsiEI CGRYCG 2 cut(s) 100, 3354
BsiHKAI GWGCWC 2 cut(s) 2718, 3171
BsiHKCI CYCGRG 1 cut(s) 688
BsiSI CCGG 2 cut(s) 1578, 3152
BslFI GGGAC 1 cut(s) 660
BsmAI GTCTC 6 cut(s) 1999, 2852, 3041, 3125, 3125, 3149
BsmBI CGTCTC 1 cut(s) 1999
BsmFI GGGAC 1 cut(s) 660
BsmI GAATGC 3 cut(s) 1368, 1509, 2255
BsnI GGCC 9 cut(s) 642, 786, 840, 1133, 1293, 2801, 3039, 3300, 3326
BsoBI CYCGRG 1 cut(s) 688
Bsp119I TTCGAA 1 cut(s) 316
Bsp120I GGGCCC 1 cut(s) 3324
Bsp1286I GDGCHC 3 cut(s) 2718, 3171, 3328
Bsp13I TCCGGA 1 cut(s) 3151
BspACI CCGC 9 cut(s) 344, 436, 469, 733, 921, 1145, 2106, 3321, 3328
BspANI GGCC 9 cut(s) 642, 786, 840, 1133, 1293, 2801, 3039, 3300, 3326
BspCNI CTCAG 8 cut(s) 399, 883, 1044, 1188, 1602, 2009, 2253, 3265
BspDI ATCGAT 1 cut(s) 2054
BspEI TCCGGA 1 cut(s) 3151
BspFNI CGCG 7 cut(s) 3211, 3213, 3240, 3268, 3270, 3330, 3332
BspHI TCATGA 2 cut(s) 2473, 3163
BspMAI CTGCAG 1 cut(s) 1404
BspMI ACCTGC 1 cut(s) 2875
BspPI GGATC 7 cut(s) 134, 1291, 1756, 2140, 2825, 3063, 3143
BspT104I TTCGAA 1 cut(s) 316
BspT107I GGYRCC 3 cut(s) 1220, 1801, 3019
BspTI CTTAAG 1 cut(s) 2996
BsrDI GCAATG 3 cut(s) 94, 456, 2260
BsrI ACTGG 5 cut(s) 416, 481, 1510, 1557, 2107
BssECI CCNNGG 7 cut(s) 564, 817, 1126, 1197, 1959, 2592, 3013
BssHII GCGCGC 3 cut(s) 3211, 3238, 3268
BssNAI GTATAC 1 cut(s) 2155
BssSI CACGAG 2 cut(s) 2550, 2717
BssT1I CCWWGG 5 cut(s) 564, 817, 1126, 1959, 3013
Bst1107I GTATAC 1 cut(s) 2155
Bst2BI CACGAG 2 cut(s) 2550, 2717
Bst2UI CCWGG 3 cut(s) 777, 1199, 3141
Bst4CI ACNGT 5 cut(s) 70, 2623, 2815, 2971, 3355
Bst6I CTCTTC 2 cut(s) 2844, 2850
BstAFI CTTAAG 1 cut(s) 2996
BstBI TTCGAA 1 cut(s) 316
BstC8I GCNNGC 6 cut(s) 1897, 1932, 3213, 3240, 3270, 3328
BstDSI CCRYGG 1 cut(s) 2592
BstENI CCTNNNNNAGG 3 cut(s) 298, 780, 2069
BstF5I GGATG 3 cut(s) 1852, 1976, 2452
BstFNI CGCG 7 cut(s) 3211, 3213, 3240, 3268, 3270, 3330, 3332
BstHHI GCGC 7 cut(s) 3213, 3215, 3240, 3242, 3270, 3272, 3332
BstMAI GTCTC 6 cut(s) 1999, 2852, 3041, 3125, 3125, 3149
BstMCI CGRYCG 2 cut(s) 100, 3354
BstMWI GCNNNNNNNGC 7 cut(s) 333, 444, 1611, 2722, 3189, 3212, 3327
BstNI CCWGG 3 cut(s) 777, 1199, 3141
BstNSI RCATGY 5 cut(s) 400, 796, 1899, 1934, 2304
BstSCI CCNGG 3 cut(s) 775, 1197, 3139
BstSFI CTRYAG 3 cut(s) 1089, 1400, 1742
BstSLI GKGCMC 1 cut(s) 3328
BstUI CGCG 7 cut(s) 3211, 3213, 3240, 3268, 3270, 3330, 3332
BstV1I GCAGC 9 cut(s) 939, 1041, 1242, 1414, 1660, 1897, 2373, 2638, 3170
BstV2I GAAGAC 2 cut(s) 1679, 2749
BstX2I RGATCY 6 cut(s) 1296, 1699, 2292, 2830, 3055, 3148
BstYI RGATCY 6 cut(s) 1296, 1699, 2292, 2830, 3055, 3148
BstZ17I GTATAC 1 cut(s) 2155
Bsu15I ATCGAT 1 cut(s) 2054
Bsu36I CCTNAGG 1 cut(s) 1309
BsuRI GGCC 9 cut(s) 642, 786, 840, 1133, 1293, 2801, 3039, 3300, 3326
BsuTUI ATCGAT 1 cut(s) 2054
BtgI CCRYGG 1 cut(s) 2592
BtsCI GGATG 3 cut(s) 1852, 1976, 2452
BtsI GCAGTG 2 cut(s) 2052, 2500
BtsIMutI CAGTG 8 cut(s) 66, 503, 1550, 2052, 2258, 2500, 2820, 2967
BveI ACCTGC 1 cut(s) 2875
Cac8I GCNNGC 6 cut(s) 1897, 1932, 3213, 3240, 3270, 3328
CaiI CAGNNNCTG 1 cut(s) 2867
CciI TCATGA 2 cut(s) 2473, 3163
CfoI GCGC 7 cut(s) 3213, 3215, 3240, 3242, 3270, 3272, 3332
Cfr13I GGNCC 8 cut(s) 674, 1131, 2203, 3037, 3086, 3143, 3324, 3325
ClaI ATCGAT 1 cut(s) 2054
CseI GACGC 1 cut(s) 3274
CsiI ACCWGGT 1 cut(s) 775
Csp6I GTAC 9 cut(s) 626, 693, 814, 822, 881, 1221, 1658, 1802, 1910
CspCI CAANNNNNGTGG 4 cut(s) 546, 581, 1846, 1881
CviQI GTAC 9 cut(s) 626, 693, 814, 822, 881, 1221, 1658, 1802, 1910
DraI TTTAAA 1 cut(s) 2764
DraIII CACNNNGTG 1 cut(s) 2973
EaeI YGGCCR 2 cut(s) 838, 2799
Eam1104I CTCTTC 2 cut(s) 2844, 2850
EarI CTCTTC 2 cut(s) 2844, 2850
EciI GGCGGA 1 cut(s) 2121
Ecl136II GAGCTC 1 cut(s) 3169
Eco130I CCWWGG 5 cut(s) 564, 817, 1126, 1959, 3013
Eco147I AGGCCT 1 cut(s) 786
Eco24I GRGCYC 2 cut(s) 3171, 3328
Eco47I GGWCC 4 cut(s) 674, 2203, 3086, 3143
Eco53kI GAGCTC 1 cut(s) 3169
Eco57I CTGAAG 6 cut(s) 474, 512, 527, 860, 1673, 3219
Eco81I CCTNAGG 1 cut(s) 1309
Eco88I CYCGRG 1 cut(s) 688
EcoICRI GAGCTC 1 cut(s) 3169
EcoNI CCTNNNNNAGG 3 cut(s) 298, 780, 2069
EcoO109I RGGNCCY 2 cut(s) 2203, 3324
EcoRI GAATTC 3 cut(s) 411, 830, 1584
EcoRII CCWGG 3 cut(s) 775, 1197, 3139
EcoT14I CCWWGG 5 cut(s) 564, 817, 1126, 1959, 3013
EcoT38I GRGCYC 2 cut(s) 3171, 3328
ErhI CCWWGG 5 cut(s) 564, 817, 1126, 1959, 3013
Esp3I CGTCTC 1 cut(s) 1999
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 1 cut(s) 660
FauI CCCGC 5 cut(s) 443, 928, 1138, 3314, 3335
FblI GTMKAC 1 cut(s) 2154
Fnu4HI GCNGC 9 cut(s) 953, 1030, 1256, 1403, 1674, 1886, 2387, 2652, 3184
FokI GGATG 3 cut(s) 1859, 1983, 2459
FriOI GRGCYC 2 cut(s) 3171, 3328
Fsp4HI GCNGC 9 cut(s) 953, 1030, 1256, 1403, 1674, 1886, 2387, 2652, 3184
FspBI CTAG 4 cut(s) 977, 1697, 2310, 2345
GlaI GCGC 7 cut(s) 3212, 3214, 3239, 3241, 3269, 3271, 3331
GluI GCNGC 9 cut(s) 953, 1030, 1256, 1403, 1674, 1886, 2387, 2652, 3184
GsaI CCCAGC 1 cut(s) 851
GsuI CTGGAG 3 cut(s) 932, 1811, 2640
HaeIII GGCC 9 cut(s) 642, 786, 840, 1133, 1293, 2801, 3039, 3300, 3326
HapII CCGG 2 cut(s) 1578, 3152
HgaI GACGC 1 cut(s) 3274
HhaI GCGC 7 cut(s) 3213, 3215, 3240, 3242, 3270, 3272, 3332
Hin6I GCGC 7 cut(s) 3211, 3213, 3238, 3240, 3268, 3270, 3330
HinP1I GCGC 7 cut(s) 3211, 3213, 3238, 3240, 3268, 3270, 3330
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 3 cut(s) 509, 652, 1427
HpaII CCGG 2 cut(s) 1578, 3152
HphI GGTGA 5 cut(s) 1312, 1325, 1465, 1808, 2570
Hpy166II GTNNAC 9 cut(s) 421, 503, 814, 1215, 1950, 2155, 2486, 2733, 2890
Hpy8I GTNNAC 9 cut(s) 421, 503, 814, 1215, 1950, 2155, 2486, 2733, 2890
Hpy99I CGWCG 1 cut(s) 2756
HpyCH4III ACNGT 5 cut(s) 70, 2623, 2815, 2971, 3355
HpyCH4IV ACGT 3 cut(s) 624, 2187, 2523
HpyF10VI GCNNNNNNNGC 7 cut(s) 333, 444, 1611, 2722, 3189, 3212, 3327
HpySE526I ACGT 3 cut(s) 624, 2187, 2523
HspAI GCGC 7 cut(s) 3211, 3213, 3238, 3240, 3268, 3270, 3330
Kpn2I TCCGGA 1 cut(s) 3151
KpnI GGTACC 2 cut(s) 1224, 1805
LmnI GCTCC 2 cut(s) 2659, 3220
Lsp1109I GCAGC 9 cut(s) 939, 1041, 1242, 1414, 1660, 1897, 2373, 2638, 3170
LweI GCATC 6 cut(s) 456, 1961, 2808, 3193, 3195, 3225
MabI ACCWGGT 1 cut(s) 775
MaeI CTAG 4 cut(s) 977, 1697, 2310, 2345
MaeII ACGT 3 cut(s) 624, 2187, 2523
MaeIII GTNAC 5 cut(s) 226, 1245, 1385, 2903, 3306
MfeI CAATTG 1 cut(s) 669
MflI RGATCY 6 cut(s) 1296, 1699, 2292, 2830, 3055, 3148
MhlI GDGCHC 3 cut(s) 2718, 3171, 3328
MlsI TGGCCA 2 cut(s) 840, 2801
MluNI TGGCCA 2 cut(s) 840, 2801
MlyI GAGTC 6 cut(s) 164, 363, 1108, 2571, 2591, 3102
MmeI TCCRAC 8 cut(s) 381, 391, 397, 2296, 3014, 3033, 3085, 3261
Mox20I TGGCCA 2 cut(s) 840, 2801
MroI TCCGGA 1 cut(s) 3151
MroXI GAANNNNTTC 3 cut(s) 574, 734, 985
MscI TGGCCA 2 cut(s) 840, 2801
Msp20I TGGCCA 2 cut(s) 840, 2801
MspA1I CMGCKG 1 cut(s) 952
MspCI CTTAAG 1 cut(s) 2996
MspI CCGG 2 cut(s) 1578, 3152
MspR9I CCNGG 3 cut(s) 777, 1199, 3141
MunI CAATTG 1 cut(s) 669
Mva1269I GAATGC 3 cut(s) 1368, 1509, 2255
MvaI CCWGG 3 cut(s) 777, 1199, 3141
MvnI CGCG 7 cut(s) 3211, 3213, 3240, 3268, 3270, 3330, 3332
MwoI GCNNNNNNNGC 7 cut(s) 333, 444, 1611, 2722, 3189, 3212, 3327
NmeAIII GCCGAG 1 cut(s) 2541
NspI RCATGY 5 cut(s) 400, 796, 1899, 1934, 2304
NspV TTCGAA 1 cut(s) 316
PaeI GCATGC 2 cut(s) 1899, 1934
PagI TCATGA 2 cut(s) 2473, 3163
PaqCI CACCTGC 1 cut(s) 2875
PauI GCGCGC 3 cut(s) 3211, 3238, 3268
PceI AGGCCT 1 cut(s) 786
PciI ACATGT 2 cut(s) 396, 2300
PctI GAATGC 3 cut(s) 1368, 1509, 2255
PdmI GAANNNNTTC 3 cut(s) 574, 734, 985
PfeI GAWTC 5 cut(s) 1153, 1274, 1653, 2109, 2281
PflMI CCANNNNNTGG 3 cut(s) 462, 1382, 3034
PfoI TCCNGGA 1 cut(s) 3139
PkrI GCNGC 9 cut(s) 954, 1031, 1257, 1404, 1675, 1887, 2388, 2653, 3185
Ple19I CGATCG 1 cut(s) 100
PleI GAGTC 6 cut(s) 164, 363, 1108, 2571, 2591, 3102
PpsI GAGTC 6 cut(s) 164, 363, 1108, 2571, 2591, 3102
PpuMI RGGWCCY 1 cut(s) 2203
PscI ACATGT 2 cut(s) 396, 2300
PshBI ATTAAT 1 cut(s) 2039
PsiI TTATAA 1 cut(s) 1010
Psp124BI GAGCTC 1 cut(s) 3171
Psp1406I AACGTT 2 cut(s) 2187, 2523
Psp5II RGGWCCY 1 cut(s) 2203
Psp6I CCWGG 3 cut(s) 775, 1197, 3139
PspFI CCCAGC 1 cut(s) 847
PspGI CCWGG 3 cut(s) 775, 1197, 3139
PspOMI GGGCCC 1 cut(s) 3324
PspPI GGNCC 8 cut(s) 674, 1131, 2203, 3037, 3086, 3143, 3324, 3325
PspPPI RGGWCCY 1 cut(s) 2203
PstI CTGCAG 1 cut(s) 1404
PstNI CAGNNNCTG 1 cut(s) 2867
PsuI RGATCY 6 cut(s) 1296, 1699, 2292, 2830, 3055, 3148
PteI GCGCGC 3 cut(s) 3211, 3238, 3268
PvuI CGATCG 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 952
RsaI GTAC 9 cut(s) 627, 694, 815, 823, 882, 1222, 1659, 1803, 1911
RsaNI GTAC 9 cut(s) 626, 693, 814, 822, 881, 1221, 1658, 1802, 1910
SacI GAGCTC 1 cut(s) 3171
SatI GCNGC 9 cut(s) 953, 1030, 1256, 1403, 1674, 1886, 2387, 2652, 3184
Sau96I GGNCC 8 cut(s) 674, 1131, 2203, 3037, 3086, 3143, 3324, 3325
SchI GAGTC 6 cut(s) 164, 363, 1108, 2571, 2591, 3102
ScrFI CCNGG 3 cut(s) 777, 1199, 3141
SduI GDGCHC 3 cut(s) 2718, 3171, 3328
SexAI ACCWGGT 1 cut(s) 775
SfaNI GCATC 6 cut(s) 456, 1961, 2808, 3193, 3195, 3225
SfcI CTRYAG 3 cut(s) 1089, 1400, 1742
SfuI TTCGAA 1 cut(s) 316
SinI GGWCC 4 cut(s) 674, 2203, 3086, 3143
SmlI CTYRAG 1 cut(s) 2996
SmoI CTYRAG 1 cut(s) 2996
SphI GCATGC 2 cut(s) 1899, 1934
SseBI AGGCCT 1 cut(s) 786
SsiI CCGC 9 cut(s) 344, 436, 469, 733, 921, 1145, 2106, 3321, 3328
SspI AATATT 3 cut(s) 340, 1381, 2437
SspMI CTAG 4 cut(s) 977, 1697, 2310, 2345
SstI GAGCTC 1 cut(s) 3171
StuI AGGCCT 1 cut(s) 786
StyD4I CCNGG 3 cut(s) 775, 1197, 3139
StyI CCWWGG 5 cut(s) 564, 817, 1126, 1959, 3013
TaaI ACNGT 5 cut(s) 70, 2623, 2815, 2971, 3355
TaiI ACGT 3 cut(s) 627, 2190, 2526
TaqI TCGA 5 cut(s) 316, 963, 2054, 2751, 2919
TfiI GAWTC 5 cut(s) 1153, 1274, 1653, 2109, 2281
TscAI CASTG 8 cut(s) 73, 510, 1557, 2052, 2265, 2507, 2820, 2974
TseI GCWGC 9 cut(s) 952, 1029, 1255, 1402, 1673, 1885, 2386, 2651, 3183
TspGWI ACGGA 4 cut(s) 163, 276, 2233, 2609
TspRI CASTG 8 cut(s) 73, 510, 1557, 2052, 2265, 2507, 2820, 2974
Van91I CCANNNNNTGG 3 cut(s) 462, 1382, 3034
Vha464I CTTAAG 1 cut(s) 2996
VpaK11BI GGWCC 4 cut(s) 674, 2203, 3086, 3143
VspI ATTAAT 1 cut(s) 2039
XagI CCTNNNNNAGG 3 cut(s) 298, 780, 2069
XbaI TCTAGA 1 cut(s) 1696
XceI RCATGY 5 cut(s) 400, 796, 1899, 1934, 2304
XcmI CCANNNNNNNNNTGG 2 cut(s) 2239, 3072
XmiI GTMKAC 1 cut(s) 2154
XmnI GAANNNNTTC 3 cut(s) 574, 734, 985
XspI CTAG 4 cut(s) 977, 1697, 2310, 2345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.