MD16G1254900.v1.1

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000671
Physical Location & Seq
Reverse (-)
29338939 .. 29342537
3599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1254900.v1.1.491

Sequence Viewer

Length: 2868 bp
ATGGAAGGAGGGTCAGCGTTCAACAAGCTCGAATACCTTTCGCTTGTCTCCAACATTTGTTCTGAACTCGAAGCTAACCTCGGCATATCCGACAGAGTCTTGGCCGAGTTCATAGCCGAGACAGGTCGCAACTCCGACGTTGTCGACGACTTCCAGGCAACCTTAAAACAAAAGGGTGCGGACATGCCGGAATATCTCAGCCGTTCCTTGTTGAAGATCATCCACGCTATTCTTCCTCCAAAGGGAAACCCCTCTCACTCTCACTCTCACAAGAACCCTGCCGCCATTGAGGAAGGAGTTGAAAGGATTCAGAGGAGCAAGAGGCAGAGGGATGACGATGACGATGACGATGTTAGGACCGGGTACCGATGTAGAAGGAGGTATTCGGATTCGGAAGAGGATGAGCAGGAGAGATGGGAGATGAAGCAGCTGATTGCCTCCGGTGTTTCGGGTGCTGCAATGCGTGATGGACACGAATTGCAATTTCGAGAAGAGGGTTTTGGGGAAGAAGAGCATGAGACCGAGTTTGATGAGGAGCAATTAGCACTTTTGCAAGGGCAGAGTGGATACTCGATTGATATGTCACCGGTTAGGATTGTGCCGAATCCCAACGGGTCATTGAGTCGTGCAGCAGCTCTTCGATCAGCGCTGCAAAAGGAGAGTAGAGAAATAACAAGAGTGGAAGAGAACATGAGGCTCAATGCAGCTCCAAAGGATTTGAATCGCGCTTGGGAAGACCCTATGCCGGGAAGGGGTGAGAGGCATTTTGCACAAGAGCTTCAGGGTGCTACTGCTGCTCGCTTTTCGGGTTATGACAGGCCCGAGTGGAACAAGAGTGCTTACGAAGGAGCCCTGGATCTTGGGCAGAGTTCAAGCCTTTCTCTTTGCGAACAGCGCCAGAGCTTACCAGTTTACAGGTTGAAGAAAGAACTGATTCAGGCTGTGAAAGAACATCGGCTTCTTGTTGTCATTGGTGAGACTGGTTCGGGTAAAACTACGCAGATAACTCAATATCTTGCTGAAGCAGGTTACACCACAAGGGGAAAAATCGGTTGCACTCAACCGCGAAGAGTTGCAGCCACGTCTGTGGCCAAGAGAGTTGCTGAGGAATTTGGTTGTCGATTAGGGGAGGAAGTTGGGTACTCTATACGATTTGATGATTGCACTGGACCGGATACTGTGATCAAGTACATGACCGATGGGACGCTTTTAAGGGAAATTTTGAGTGATAAAAACCTGTCTCAGTACTCCGTGATCATCCTTGATGAAGCTCATGAAAGAACAGTCTCCACTGACGTTCTTTTCGGATTGCTGAAGCAACTTGTTGAAAGGCGACCTGATCTTCGCTTGATTGTTACTTCTGCCACATTGGATGCAGTGAAGTTTTCAGATTATTTCTTTCATTGTCAGATATTTACGATTCCTGGTAGAACTTATCCGGTAGAATTAATGTACTCGAAACAGCCAGAAAGTGATTACTTGGATGCAGCTATATTGACTGTTTTGCAACTTCACTTGACTAAACCCGAAGGTGACATACTTGTGTTCCTGACGGGTGAGGAGGAGATTGAATTTGCTTGTCAGTCTCTGTATGAAAGGATGAAAGTACTCGGTAGAGAAGCTCCGGAATTGATTATTCTACCAGCTTATGGTGCCCAACCGAGTGCAGAGCAATCGAAGATATTCAAGCCTGCTCCTTCAGGGAAGAGAAAAGTGGTTGTTGCTACCAATATTGCTGAAGCCTCTTTGACCATTGATGGGATATACTACGTAATTGATCCGGGATTTGCAAAGCAAAATGTATTTAACCCAAAGCTTGGGATTGATTTACTGGTTGTAACTCCAATTTCTCAAGCATCAGCCAAACAACGAGCTGGGCGCGCTGGGCGGACAGGAGCTGGGGAATGTTACCGCCTTTACACTGAGAGCGCTTTTCACAATGAGATGTCTCCTACCACGGTTCCAGAAATCCTAAGAACAAATCTTGTGCATACTACACTTATGATGAAAGCAATGGGGATACACGACCTCAAATCTTTTGATTTTATGGATCCACCGTCATCCCAGGCGCTCGAGTCTGCCATGAAACAGCTGCAAAGTCTAGGAGCTTTGGATGAGGAGGGGTTACTGACCAAATTGGGTAGAAGAATGGCAGTGTTGCCTCTGGATCCACCTTTATCGAAAATGCTGCTTGCCAGTGCGAACCTTGGATGCAGTGATGAGATTGTGACAATCATTGCCATGCTTCAGGCCGGAAATATCTTCTACAGGCCTAGAGCAAAACAAAGCCAAGCAGATCAAAAGAAGGCCAAGTTTTTCCAGCCTGAGGGAGACCATCTGACTTTGCTCACTGTTTACGAGGCTTGGAAAGCCAAGGATTTTTCTAAACCCTGGTGTTCTGAGAACTTTTTACAGTTTCGGTCGTTGAGCAGGGCAAGGGATATCAGGAAACAGCTTCTTACTGTCATGGGCAAGTATGAATTAGAGGTTGTAAGTGCAGGAAGAAATGTCGTAATGGTCAGAAAGGCAATAATTGCTGGATGCTTCTTCAGAGTAGCAAGAAAGGATCCAAAGGAGGGTTATAGAACCTTAGTCGATAATCAGCAAGTTTATATCCACCCGAGCAGCGTTCTTTTCCAGAGACAACCAGCTTGGGTCATCTACAATGAGCTTGTGATGACCACGAAGGAGTACATGCGTGAGGTCAGTGCCATAGAACCCATGTGGCTGATGGAGCAGGTTCCAAGATTCTTTAAGGCGGCAAATCCTACAAAGATGAGCAACGGAAAACGTCAAGAACGTACTGAACCACTGTATGACAGATATCATGAGCCCAACTCATGGCGCCTGAGCAGAAGGCATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

956

Amino Acids

107.67

Weight (kDa)

6.23

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 308 - 458 1.8e-09 DEAD/DEAH box helicase
AAA_22 PF13401 317 - 450 1.6e-07 AAA domain
Helicase_C PF00271 501 - 632 4.4e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 694 - 722 2.2e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 723 - 782 2.2e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 840 - 915 6.3e-27 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1016, 2728
Acc65I GGTACC 1 cut(s) 363
AccB1I GGYRCC 3 cut(s) 363, 1652, 2844
AccB7I CCANNNNNTGG 3 cut(s) 1649, 1817, 2841
AccI GTMKAC 1 cut(s) 144
AccII CGCG 3 cut(s) 726, 1066, 1881
AccIII TCCGGA 1 cut(s) 1624
AciI CCGC 6 cut(s) 179, 282, 1064, 1888, 1912, 2759
AclWI GGATC 8 cut(s) 864, 1772, 2045, 2058, 2162, 2175, 2561, 2574
AcoI YGGCCR 2 cut(s) 102, 1089
AcsI RAATTY 3 cut(s) 1109, 1218, 1571
AcuI CTGAAG 7 cut(s) 764, 1041, 1334, 1683, 1758, 2231, 2533
AcyI GRCGYC 1 cut(s) 2845
AfaI GTAC 8 cut(s) 365, 1142, 1190, 1247, 1454, 1608, 2693, 2803
AfeI AGCGCT 2 cut(s) 648, 1930
AgeI ACCGGT 1 cut(s) 586
AgsI TTSAA 9 cut(s) 22, 214, 302, 721, 873, 922, 1328, 1571, 1687
AjiI CACGTC 1 cut(s) 1083
AjnI CCWGG 5 cut(s) 153, 852, 1423, 2064, 2390
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
AleI CACNNNNGTG 1 cut(s) 1085
AlwI GGATC 8 cut(s) 864, 1772, 2045, 2058, 2162, 2175, 2561, 2574
AlwNI CAGNNNCTG 2 cut(s) 1588, 1898
Ama87I CYCGRG 3 cut(s) 821, 2072, 2620
Aor13HI TCCGGA 1 cut(s) 1624
Aor51HI AGCGCT 2 cut(s) 648, 1930
AoxI GGCC 6 cut(s) 102, 818, 1089, 2250, 2270, 2307
ApoI RAATTY 3 cut(s) 1109, 1218, 1571
ArsI GACNNNNNNTTYG 4 cut(s) 1286, 1318, 1326, 1358
AseI ATTAAT 1 cut(s) 1448
AsiGI ACCGGT 1 cut(s) 586
Asp700I GAANNNNTTC 3 cut(s) 306, 933, 1682
Asp718I GGTACC 1 cut(s) 363
AspLEI GCGC 8 cut(s) 649, 728, 897, 1881, 1883, 1931, 2071, 2847
AspS9I GGNCC 3 cut(s) 357, 819, 1169
AsuC2I CCSGG 3 cut(s) 361, 747, 1782
AsuHPI GGTGA 5 cut(s) 576, 767, 986, 1544, 1568
AvaI CYCGRG 3 cut(s) 821, 2072, 2620
AvaII GGWCC 2 cut(s) 357, 1169
AxyI CCTNAGG 1 cut(s) 2325
BaeGI GKGCMC 1 cut(s) 1657
BaeI ACNNNNGTAYC 2 cut(s) 355, 388
BalI TGGCCA 1 cut(s) 1091
BamHI GGATCC 3 cut(s) 2050, 2167, 2566
BanI GGYRCC 3 cut(s) 363, 1652, 2844
BanII GRGCYC 2 cut(s) 853, 2835
BarI GAAGNNNNNNTAC 2 cut(s) 1521, 1553
BbsI GAAGAC 1 cut(s) 741
BbvCI CCTCAGC 1 cut(s) 1104
BccI CCATC 6 cut(s) 408, 461, 1193, 1751, 2343, 2725
BceAI ACGGC 1 cut(s) 186
BcgI CGANNNNNNTGC 4 cut(s) 1656, 1690, 2170, 2204
BciT130I CCWGG 5 cut(s) 155, 854, 1425, 2066, 2392
BciVI GTATCC 3 cut(s) 560, 1168, 2013
BclI TGATCA 2 cut(s) 1182, 1254
BcnI CCSGG 3 cut(s) 361, 747, 1782
BfaI CTAG 2 cut(s) 2102, 2274
BfmI CTRYAG 1 cut(s) 2266
BfoI RGCGCY 5 cut(s) 650, 898, 1932, 2072, 2848
BfuAI ACCTGC 2 cut(s) 1016, 2728
BfuI GTATCC 3 cut(s) 560, 1168, 2013
BmcAI AGTACT 2 cut(s) 1247, 1608
Bme1390I CCNGG 8 cut(s) 155, 361, 747, 854, 1425, 1782, 2066, 2392
Bme18I GGWCC 2 cut(s) 357, 1169
BmeT110I CYCGRG 3 cut(s) 821, 2072, 2620
BmgBI CACGTC 1 cut(s) 1083
BmgT120I GGNCC 3 cut(s) 357, 819, 1169
BmiI GGNNCC 9 cut(s) 365, 850, 1654, 1962, 2052, 2169, 2568, 2742, 2846
BmrFI CCNGG 8 cut(s) 155, 361, 747, 854, 1425, 1782, 2066, 2392
BmsI GCATC 5 cut(s) 1363, 1474, 1865, 2201, 2531
BpiI GAAGAC 1 cut(s) 741
BplI GAGNNNNNCTC 2 cut(s) 2822, 2854
Bpu10I CCTNAGC 2 cut(s) 1104, 2849
BpuEI CTTGAG 1 cut(s) 1836
BpuMI CCSGG 3 cut(s) 361, 747, 1782
BsaAI YACGTR 1 cut(s) 1771
BsaBI GATNNNNATC 1 cut(s) 720
BsaHI GRCGYC 1 cut(s) 2845
BsaI GGTCTC 2 cut(s) 512, 2325
BsaJI CCNNGG 7 cut(s) 79, 852, 1956, 2064, 2206, 2373, 2390
BsaWI WCCGGW 5 cut(s) 440, 586, 1171, 1438, 1624
Bse118I RCCGGY 1 cut(s) 586
Bse1I ACTGG 5 cut(s) 908, 985, 1171, 1836, 2196
Bse21I CCTNAGG 1 cut(s) 2325
Bse3DI GCAATG 3 cut(s) 465, 2019, 2235
Bse8I GATNNNNATC 1 cut(s) 720
BseAI TCCGGA 1 cut(s) 1624
BseBI CCWGG 5 cut(s) 155, 854, 1425, 2066, 2392
BseDI CCNNGG 7 cut(s) 79, 852, 1956, 2064, 2206, 2373, 2390
BseJI GATNNNNATC 1 cut(s) 720
BseMI GCAATG 3 cut(s) 465, 2019, 2235
BseMII CTCAG 7 cut(s) 211, 1095, 1256, 1914, 2316, 2391, 2840
BseNI ACTGG 5 cut(s) 908, 985, 1171, 1836, 2196
BsePI GCGCGC 1 cut(s) 1879
BseRI GAGGAG 5 cut(s) 328, 548, 1574, 1577, 2132
BseSI GKGCMC 1 cut(s) 1657
BseYI CCCAGC 3 cut(s) 1874, 1883, 1898
BsgI GTGCAG 3 cut(s) 648, 1686, 2517
Bsh1236I CGCG 3 cut(s) 726, 1066, 1881
Bsh1285I CGRYCG 1 cut(s) 2423
BshFI GGCC 6 cut(s) 104, 820, 1091, 2252, 2272, 2309
BshNI GGYRCC 3 cut(s) 363, 1652, 2844
BshTI ACCGGT 1 cut(s) 586
BsiEI CGRYCG 1 cut(s) 2423
BsiHKCI CYCGRG 3 cut(s) 821, 2072, 2620
BslFI GGGAC 1 cut(s) 1216
BsmFI GGGAC 1 cut(s) 1216
BsnI GGCC 6 cut(s) 104, 820, 1091, 2252, 2272, 2309
Bso31I GGTCTC 2 cut(s) 512, 2325
BsoBI CYCGRG 3 cut(s) 821, 2072, 2620
Bsp1286I GDGCHC 3 cut(s) 853, 1657, 2835
Bsp13I TCCGGA 1 cut(s) 1624
BspACI CCGC 6 cut(s) 179, 282, 1064, 1888, 1912, 2759
BspANI GGCC 6 cut(s) 104, 820, 1091, 2252, 2272, 2309
BspCNI CTCAG 7 cut(s) 210, 1096, 1255, 1915, 2317, 2392, 2841
BspEI TCCGGA 1 cut(s) 1624
BspFNI CGCG 3 cut(s) 726, 1066, 1881
BspHI TCATGA 2 cut(s) 1273, 2827
BspLI GGNNCC 9 cut(s) 365, 850, 1654, 1962, 2052, 2169, 2568, 2742, 2846
BspMI ACCTGC 2 cut(s) 1016, 2728
BspPI GGATC 8 cut(s) 864, 1772, 2045, 2058, 2162, 2175, 2561, 2574
BspQI GCTCTTC 2 cut(s) 504, 642
BspT107I GGYRCC 3 cut(s) 363, 1652, 2844
BspTNI GGTCTC 2 cut(s) 512, 2325
BsrDI GCAATG 3 cut(s) 465, 2019, 2235
BsrFI RCCGGY 1 cut(s) 586
BsrI ACTGG 5 cut(s) 908, 985, 1171, 1836, 2196
BssAI RCCGGY 1 cut(s) 586
BssECI CCNNGG 7 cut(s) 79, 852, 1956, 2064, 2206, 2373, 2390
BssHII GCGCGC 1 cut(s) 1879
BssNI GRCGYC 1 cut(s) 2845
BssT1I CCWWGG 2 cut(s) 2206, 2373
Bst2UI CCWGG 5 cut(s) 155, 854, 1425, 2066, 2392
Bst4CI ACNGT 9 cut(s) 1180, 1285, 1501, 1960, 2058, 2353, 2415, 2464, 2814
Bst6I CTCTTC 7 cut(s) 390, 486, 504, 642, 678, 1063, 1700
BstACI GRCGYC 1 cut(s) 2845
BstAPI GCANNNNNTGC 1 cut(s) 2534
BstBAI YACGTR 1 cut(s) 1771
BstC8I GCNNGC 5 cut(s) 799, 1692, 1881, 2193, 2862
BstDEI CTNAG 9 cut(s) 197, 1104, 1242, 1923, 1973, 2325, 2400, 2590, 2849
BstDSI CCRYGG 1 cut(s) 1956
BstFNI CGCG 3 cut(s) 726, 1066, 1881
BstH2I RGCGCY 5 cut(s) 650, 898, 1932, 2072, 2848
BstHHI GCGC 8 cut(s) 649, 728, 897, 1881, 1883, 1931, 2071, 2847
BstMCI CGRYCG 1 cut(s) 2423
BstMWI GCNNNNNNNGC 8 cut(s) 794, 894, 1652, 1880, 1885, 2369, 2534, 2734
BstNI CCWGG 5 cut(s) 155, 854, 1425, 2066, 2392
BstNSI RCATGY 3 cut(s) 187, 2698, 2864
BstSCI CCNGG 8 cut(s) 153, 359, 745, 852, 1423, 1780, 2064, 2390
BstSFI CTRYAG 1 cut(s) 2266
BstSLI GKGCMC 1 cut(s) 1657
BstSNI TACGTA 1 cut(s) 1771
BstUI CGCG 3 cut(s) 726, 1066, 1881
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 4 cut(s) 856, 2050, 2167, 2566
BstXI CCANNNNNNTGG 1 cut(s) 1087
BstYI RGATCY 4 cut(s) 856, 2050, 2167, 2566
Bsu36I CCTNAGG 1 cut(s) 2325
BsuI GTATCC 3 cut(s) 560, 1168, 2013
BsuRI GGCC 6 cut(s) 104, 820, 1091, 2252, 2272, 2309
BtgI CCRYGG 1 cut(s) 1956
BtrI CACGTC 1 cut(s) 1083
BtsI GCAGTG 3 cut(s) 1383, 2160, 2221
BveI ACCTGC 2 cut(s) 1016, 2728
Cac8I GCNNGC 5 cut(s) 799, 1692, 1881, 2193, 2862
CaiI CAGNNNCTG 2 cut(s) 1588, 1898
CciI TCATGA 2 cut(s) 1273, 2827
CfoI GCGC 8 cut(s) 649, 728, 897, 1881, 1883, 1931, 2071, 2847
Cfr10I RCCGGY 1 cut(s) 586
Cfr13I GGNCC 3 cut(s) 357, 819, 1169
CseI GACGC 1 cut(s) 1213
Csp6I GTAC 8 cut(s) 364, 1141, 1189, 1246, 1453, 1607, 2692, 2802
CspAI ACCGGT 1 cut(s) 586
CviQI GTAC 8 cut(s) 364, 1141, 1189, 1246, 1453, 1607, 2692, 2802
DdeI CTNAG 9 cut(s) 197, 1104, 1242, 1923, 1973, 2325, 2400, 2590, 2849
DinI GGCGCC 1 cut(s) 2846
EaeI YGGCCR 2 cut(s) 102, 1089
Eam1104I CTCTTC 7 cut(s) 390, 486, 504, 642, 678, 1063, 1700
EarI CTCTTC 7 cut(s) 390, 486, 504, 642, 678, 1063, 1700
EciI GGCGGA 1 cut(s) 1903
Eco105I TACGTA 1 cut(s) 1771
Eco130I CCWWGG 2 cut(s) 2206, 2373
Eco147I AGGCCT 1 cut(s) 2272
Eco24I GRGCYC 2 cut(s) 853, 2835
Eco31I GGTCTC 2 cut(s) 512, 2325
Eco32I GATATC 2 cut(s) 2443, 2825
Eco47I GGWCC 2 cut(s) 357, 1169
Eco47III AGCGCT 2 cut(s) 648, 1930
Eco57I CTGAAG 7 cut(s) 764, 1041, 1334, 1683, 1758, 2231, 2533
Eco81I CCTNAGG 1 cut(s) 2325
Eco88I CYCGRG 3 cut(s) 821, 2072, 2620
EcoRII CCWGG 5 cut(s) 153, 852, 1423, 2064, 2390
EcoRV GATATC 2 cut(s) 2443, 2825
EcoT14I CCWWGG 2 cut(s) 2206, 2373
EcoT38I GRGCYC 2 cut(s) 853, 2835
EgeI GGCGCC 1 cut(s) 2846
EheI GGCGCC 1 cut(s) 2846
ErhI CCWWGG 2 cut(s) 2206, 2373
FalI AAGNNNNNCTT 1 cut(s) 2848
FaqI GGGAC 1 cut(s) 1216
FbaI TGATCA 2 cut(s) 1182, 1254
FblI GTMKAC 1 cut(s) 144
FriOI GRGCYC 2 cut(s) 853, 2835
FspBI CTAG 2 cut(s) 2102, 2274
GlaI GCGC 8 cut(s) 648, 727, 896, 1880, 1882, 1930, 2070, 2846
GsaI CCCAGC 3 cut(s) 1878, 1887, 1902
HaeII RGCGCY 5 cut(s) 650, 898, 1932, 2072, 2848
HaeIII GGCC 6 cut(s) 104, 820, 1091, 2252, 2272, 2309
HgaI GACGC 1 cut(s) 1213
HhaI GCGC 8 cut(s) 649, 728, 897, 1881, 1883, 1931, 2071, 2847
Hin1I GRCGYC 1 cut(s) 2845
Hin6I GCGC 8 cut(s) 647, 726, 895, 1879, 1881, 1929, 2069, 2845
HinP1I GCGC 8 cut(s) 647, 726, 895, 1879, 1881, 1929, 2069, 2845
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 1814
HphI GGTGA 5 cut(s) 576, 767, 986, 1544, 1568
Hpy166II GTNNAC 3 cut(s) 145, 913, 2356
Hpy8I GTNNAC 3 cut(s) 145, 913, 2356
Hpy99I CGWCG 2 cut(s) 140, 149
HpyAV CCTTC 9 cut(s) 287, 369, 744, 839, 1523, 1707, 2299, 2680, 2850
HpyCH4III ACNGT 9 cut(s) 1180, 1285, 1501, 1960, 2058, 2353, 2415, 2464, 2814
HpyCH4IV ACGT 6 cut(s) 138, 1082, 1296, 1770, 2791, 2800
HpyF10VI GCNNNNNNNGC 8 cut(s) 794, 894, 1652, 1880, 1885, 2369, 2534, 2734
HpyF3I CTNAG 9 cut(s) 197, 1104, 1242, 1923, 1973, 2325, 2400, 2590, 2849
HpySE526I ACGT 6 cut(s) 138, 1082, 1296, 1770, 2791, 2800
Hsp92I GRCGYC 1 cut(s) 2845
HspAI GCGC 8 cut(s) 647, 726, 895, 1879, 1881, 1929, 2069, 2845
KasI GGCGCC 1 cut(s) 2844
Kpn2I TCCGGA 1 cut(s) 1624
KpnI GGTACC 1 cut(s) 367
Ksp22I TGATCA 2 cut(s) 1182, 1254
LguI GCTCTTC 2 cut(s) 504, 642
LmnI GCTCC 9 cut(s) 315, 535, 712, 848, 1627, 1699, 1895, 2105, 2734
LweI GCATC 5 cut(s) 1363, 1474, 1865, 2201, 2531
MaeI CTAG 2 cut(s) 2102, 2274
MaeII ACGT 6 cut(s) 138, 1082, 1296, 1770, 2791, 2800
MaeIII GTNAC 8 cut(s) 582, 1028, 1354, 1532, 1837, 1907, 2124, 2227
MflI RGATCY 4 cut(s) 856, 2050, 2167, 2566
MhlI GDGCHC 3 cut(s) 853, 1657, 2835
MlsI TGGCCA 1 cut(s) 1091
MluNI TGGCCA 1 cut(s) 1091
Mly113I GGCGCC 1 cut(s) 2845
MlyI GAGTC 3 cut(s) 105, 631, 2084
MmeI TCCRAC 3 cut(s) 75, 114, 159
Mox20I TGGCCA 1 cut(s) 1091
MroI TCCGGA 1 cut(s) 1624
MroXI GAANNNNTTC 3 cut(s) 306, 933, 1682
MscI TGGCCA 1 cut(s) 1091
MseI TTAA 5 cut(s) 164, 1211, 1448, 1806, 2754
MslI CAYNNNNRTG 3 cut(s) 1085, 1541, 2240
Msp20I TGGCCA 1 cut(s) 1091
MspA1I CMGCKG 2 cut(s) 430, 2092
MspR9I CCNGG 8 cut(s) 155, 361, 747, 854, 1425, 1782, 2066, 2392
MvaI CCWGG 5 cut(s) 155, 854, 1425, 2066, 2392
MvnI CGCG 3 cut(s) 726, 1066, 1881
MwoI GCNNNNNNNGC 8 cut(s) 794, 894, 1652, 1880, 1885, 2369, 2534, 2734
NarI GGCGCC 1 cut(s) 2845
NciI CCSGG 3 cut(s) 361, 747, 1782
NlaIV GGNNCC 9 cut(s) 365, 850, 1654, 1962, 2052, 2169, 2568, 2742, 2846
NmeAIII GCCGAG 3 cut(s) 60, 130, 142
NmuCI GTSAC 3 cut(s) 582, 1532, 2227
NspI RCATGY 3 cut(s) 187, 2698, 2864
OliI CACNNNNGTG 1 cut(s) 1085
PaeI GCATGC 1 cut(s) 2864
PaeR7I CTCGAG 1 cut(s) 2072
PagI TCATGA 2 cut(s) 1273, 2827
PauI GCGCGC 1 cut(s) 1879
PceI AGGCCT 1 cut(s) 2272
PciSI GCTCTTC 2 cut(s) 504, 642
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 144, 2797
PdmI GAANNNNTTC 3 cut(s) 306, 933, 1682
PfeI GAWTC 7 cut(s) 307, 389, 604, 721, 934, 1420, 2748
PflFI GACNNNGTC 2 cut(s) 95, 140
PflMI CCANNNNNTGG 3 cut(s) 1649, 1817, 2841
PfoI TCCNGGA 1 cut(s) 1780
PinAI ACCGGT 1 cut(s) 586
PleI GAGTC 3 cut(s) 104, 630, 2083
PluTI GGCGCC 1 cut(s) 2848
PpsI GAGTC 3 cut(s) 104, 630, 2083
Ppu21I YACGTR 1 cut(s) 1771
PshBI ATTAAT 1 cut(s) 1448
Psp6I CCWGG 5 cut(s) 153, 852, 1423, 2064, 2390
PspFI CCCAGC 3 cut(s) 1874, 1883, 1898
PspGI CCWGG 5 cut(s) 153, 852, 1423, 2064, 2390
PspN4I GGNNCC 9 cut(s) 365, 850, 1654, 1962, 2052, 2169, 2568, 2742, 2846
PspPI GGNCC 3 cut(s) 357, 819, 1169
PspXI VCTCGAGB 1 cut(s) 2072
PstNI CAGNNNCTG 2 cut(s) 1588, 1898
PsuI RGATCY 4 cut(s) 856, 2050, 2167, 2566
PsyI GACNNNGTC 2 cut(s) 95, 140
PteI GCGCGC 1 cut(s) 1879
PvuII CAGCTG 2 cut(s) 430, 2092
RsaI GTAC 8 cut(s) 365, 1142, 1190, 1247, 1454, 1608, 2693, 2803
RsaNI GTAC 8 cut(s) 364, 1141, 1189, 1246, 1453, 1607, 2692, 2802
RseI CAYNNNNRTG 3 cut(s) 1085, 1541, 2240
SalI GTCGAC 1 cut(s) 143
SapI GCTCTTC 2 cut(s) 504, 642
SaqAI TTAA 5 cut(s) 164, 1211, 1448, 1806, 2754
Sau96I GGNCC 3 cut(s) 357, 819, 1169
ScaI AGTACT 2 cut(s) 1247, 1608
SchI GAGTC 3 cut(s) 105, 631, 2084
ScrFI CCNGG 8 cut(s) 155, 361, 747, 854, 1425, 1782, 2066, 2392
SduI GDGCHC 3 cut(s) 853, 1657, 2835
SfaNI GCATC 5 cut(s) 1363, 1474, 1865, 2201, 2531
SfcI CTRYAG 1 cut(s) 2266
SfoI GGCGCC 1 cut(s) 2846
Sfr274I CTCGAG 1 cut(s) 2072
SinI GGWCC 2 cut(s) 357, 1169
SlaI CTCGAG 1 cut(s) 2072
SmiMI CAYNNNNRTG 3 cut(s) 1085, 1541, 2240
SmlI CTYRAG 2 cut(s) 1851, 2072
SmoI CTYRAG 2 cut(s) 1851, 2072
SnaBI TACGTA 1 cut(s) 1771
SphI GCATGC 1 cut(s) 2864
SseBI AGGCCT 1 cut(s) 2272
SsiI CCGC 6 cut(s) 179, 282, 1064, 1888, 1912, 2759
SspDI GGCGCC 1 cut(s) 2844
SspI AATATT 1 cut(s) 1732
SspMI CTAG 2 cut(s) 2102, 2274
StuI AGGCCT 1 cut(s) 2272
StyD4I CCNGG 8 cut(s) 153, 359, 745, 852, 1423, 1780, 2064, 2390
StyI CCWWGG 2 cut(s) 2206, 2373
TaaI ACNGT 9 cut(s) 1180, 1285, 1501, 1960, 2058, 2353, 2415, 2464, 2814
TaiI ACGT 6 cut(s) 141, 1085, 1299, 1773, 2794, 2803
TaqII GACCGA 3 cut(s) 536, 1211, 2409
TatI WGTACW 5 cut(s) 1188, 1245, 1452, 1606, 2691
TauI GCSGC 2 cut(s) 284, 2762
TfiI GAWTC 7 cut(s) 307, 389, 604, 721, 934, 1420, 2748
Tru1I TTAA 5 cut(s) 164, 1211, 1448, 1806, 2754
Tru9I TTAA 5 cut(s) 164, 1211, 1448, 1806, 2754
TseFI GTSAC 3 cut(s) 582, 1532, 2227
Tsp45I GTSAC 3 cut(s) 582, 1532, 2227
TspGWI ACGGA 2 cut(s) 1240, 2799
Tth111I GACNNNGTC 2 cut(s) 95, 140
Van91I CCANNNNNTGG 3 cut(s) 1649, 1817, 2841
VpaK11BI GGWCC 2 cut(s) 357, 1169
VspI ATTAAT 1 cut(s) 1448
XapI RAATTY 3 cut(s) 1109, 1218, 1571
XceI RCATGY 3 cut(s) 187, 2698, 2864
XcmI CCANNNNNNNNNTGG 1 cut(s) 2728
XhoI CTCGAG 1 cut(s) 2072
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 3 cut(s) 306, 933, 1682
XspI CTAG 2 cut(s) 2102, 2274
ZrmI AGTACT 2 cut(s) 1247, 1608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.