MD09G1231900.v1.1

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000671
Physical Location & Seq
Forward (+)
28757557 .. 28762133
4577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1231900.v1.1.491

Sequence Viewer

Length: 3612 bp
ATGGCGGCTGCCGACGACGGTCTGAAGAAGTTGGAGTACCTTTCCCTGGTCTCCAAAGTCTGTTCAGAGCTCGAAACGCATCTAGGGTTTGGGGACAAAGTTCTGGCGGAGTTCATCACCGAAATCGGACGGAGCTGTGAGACGGTGGACGAATTCGACACCCGATTGAAGGAGAACGGCGCCGAGATGCCGGACTACTTTGTCCGTACACTCATCACTATCATCCACGCAATCCTTCCTCCGAAGCCGAAGCCGAAGAAGGATGCTTCGAAGGAAGACGGCACTTCTGAAGGTAAGAAAACCAAGTTTAAGGCTTTGGCTATTGCTGATAATAGAGATAGAGTCAAGGAAATTGATAAGGAAATTGAGCTGGAGACCAGGGAGAAGCACACCGGCTTAGAGGAGTGGGATGGAGAGCGCGAAGACAATAGGCAGAGGGGTAGAGATAGATATAGGGATGGAGATAGAGATAGAAATAGAGATAGAGATCGAGATCGTGATAGGCACAGAGCTAGGGATAGAGGTCGGAGAGACAGGGGAGGGGATAAGCATGACAGAGACGATAATCATAGAGATTCACAATATAATGATGATGACGGGGAAAGAAGGAGGTACACAGATCGATACGATAAGCATAAGAGAGATGGGTGTGAAGAGAATGGAAATGATAGGGTGGATGAAGATGCTAGGAGAGGTAATAGGGATAGGCAGAATGGTCCTAGTAAGAACCTTTCCGATGAGCCTGAATTGTACCAGGTGTATAGGGGTAGGGTTTCAAGAGTGATGGACACTGGTTGCTTTGTTCAGTTAAATGATTTGAGAGGGAAGGAAGGTTTGGTTCATGTTTCACAGATGGCAACTCGGCGAATTAGTAATGCTAAGGATGTGGTGAAGCGGGATCAGGAAGTTTATGTGAAGGTGATTTCGGTTTCGGGTCAGAAGTTGAGCCTTTCAATGAGGGATGTTGATCAGCATTCTGGGAAGGATTTGCTTCCATTGAAGAAGAGCTCAGAGGATGATGCTCATAGGACCAATATGTCAGTGTCCAGAGACGGACCTGTGACTAGGACGGGTCTTTCTGGGATTAGGATTGTGGAAGAGGATGATGTTGGTCCATCACGCCGGCCATTGAAGAGAATGAGTTCGCCTGAGAAGTGGGAAGCCAAACAGTTAATTGCCTCAGGTGTTTTGAGTGTGACGGAGTACCCAATGTATGATGACGAAGCAGATGGGATGCTATACCAGGAAGAGGGAGCTGAAGAAGAGCTTGAGATTGAGCTCAATGAGGATGAGCCAGCCTTTTTGAATGGGCAAACCAGGTATTCAGTGGATATGTCGCCTGTCAAAATCTTTAAGAATCCCGAAGGGTCTTTGAGTCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGAGAAGTGCGAGAGCAGCAGCAAAGAACCATGTTGGATTCCATCCCAAAGGATCTGAATCGTCCCTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCCCAGGAGCTTAGAGGTGTTGGTTTGTCTGCGTATGATATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAAACCATCAGCTTTGGGCAGAGGTCCAAGCTATCAATTCAGGAACAGAGACAGAGCTTGCCTATATACAAGCTGAAGAAAGAATTGGTTCAGGCAGTGAATGAGAATCAAGTGCTCGTCGTCATTGGTGAGACTGGTTCTGGTAAGACAACCCAGGTAACACAGTATCTTGCAGAAGCGGGTTACACGACAAAGGGCAAGATTGGATGTACACAGCCCCGTAGGGTTGCTGCAACGTCTGTAGCCAAGAGGGTGGCTGAAGAGTTTGGTTGTCGTTTGGGGGAGGAAGTTGGCTATGCTATTCGTTTTGAGGATTGCACTGGACCAGATACTGTCATCAAGTACATGACCGATGGTATGCTTCTTAGGGAGATTTTGATTGATGAGAACCTTTCCCAATACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCAACACGGACGTTCTTTTTGGATTACTGAAGCAGCTTGTGAAGCAGAGACCAGACCTTCGTTTGATTGTCACCTCTGCTACTTTGGATGCAGAGAAGTTTTCAGGTTATTTCTTCAACTGTAACATCTTCACTATCCCCGGGAGAACTTTTCCTGTCGAGATACTCTACACCAAGCAGCCAGAAAGTGATTACCTTGATGCAGCTTTGATCACTGTTCTACAGATCCACTTAACAGAACCTGAAGGTGACATCCTTCTCTTCCTGACTGGACAAGAAGAGATTGATTTTGCATGCCAGTCTCTTTATGAGAGGATGAAAGGTCTAGGTAAAAATGTTCCTGAGTTAATTATATTACCAGTATATAGTGCCCTTCCTAGTGAAATGCAGTCAAGGATATTTGATCCTGCCCCACCAGGGAAGAGGAAAGTGGTTGTGGCTACTAATATTGCTGAAGCATCGCTGACCATTGATGGGATATTTTATGTCATTGATCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTAGATTCACTTGTCATTACTCCAATTTCACAAGCATCAGCCAAGCAACGAGCTGGGCGAGCCGGACGCACAGGGCCTGGGAAATGTTACCGTCTCTACACTGAGAGTGCATACCGCAATGAAATGTCCCCTACTTCAATTCCAGAAATCCAGAGGATAAATCTTGGAACTACCACCCTCACGATGAAAGCCATCGGGATAAATGATCTCCTCTCTTTTGATTTTATGGATCCTCCTTCACCCCAAGCACTCATTTCTGCCATGGAACAACTGTACAGTTTAGGAGCACTGGATGAGGAGGGGCTGCTGACCAAATTGGGCAGGAAAATGGCTGAGTTTCCGCTTGATCCACCGTTATCTAAAATGCTTCTCGCCAGTGTGGACCTCGGATGTAGTGATGAGATCTTGACCATCATTGCAATGATTCAGACTGGCAATATCTTTTACAGGCCTAGGGAAAAACAAGCCCAGGCTGATCAGAAGAGAGCTAAGTTTTTCCAGCCAGAGGGAGACCATCTGACTTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAATTTCTCAGGTCCATGGTGTTTTGAGAACTTTGTTCAGTCCCGATCCTTGAGGAGGGCACAGGATGTCAGAAAACAGCTTCTGAGCATCATGGACAAGTATAAATTGGACGTGGTCAGTGCAGGAAAGAATTTTACAAAGATCAGGAAGGCCATTACAGCAGGTTTTTTCTTCCATGGTGCTAGAAAGGACCCACAGGAGGGTTATAGAACCCTAGTCGAGAACCAGCCAGTTTATATTCACCCAAGCAGTGCTCTCTTCCAGAGACAACCAGATTGGGTCATCTACCATGAGCTGGTTATGACTACAAAGGAGTACATGCGCGAGGTGATGGTCGTCGACCCCAAATGGCTGGTAGAACTGGCTCCAAGGTTCTTCAAAGTATCAGATCCTACAAAGATGAGTAAGCGCAAGCGTCAGGAACGAGTTGAACCCTTATATGACAGGTACCATGAACCTAACTCTTGGCGTCTTAGTAAGCGTCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1204

Amino Acids

137.18

Weight (kDa)

6.34

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 249 - 319 5.5e-12 S1 RNA binding domain
DEAD PF00270 557 - 706 4.4e-08 DEAD/DEAH box helicase
AAA_22 PF13401 566 - 698 2.9e-06 AAA domain
Helicase_C PF00271 750 - 880 9.8e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 942 - 970 8.3e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 971 - 1030 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1088 - 1164 3.5e-25 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2550
AasI GACNNNNNNGTC 2 cut(s) 200, 1036
Acc36I ACCTGC 1 cut(s) 3272
Acc65I GGTACC 1 cut(s) 3567
AccB1I GGYRCC 2 cut(s) 179, 3567
AccB7I CCANNNNNTGG 2 cut(s) 1505, 3415
AccI GTMKAC 1 cut(s) 3459
AccII CGCG 2 cut(s) 420, 3444
AciI CCGC 6 cut(s) 5, 107, 895, 1769, 2680, 2906
AcoI YGGCCR 1 cut(s) 1124
AcsI RAATTY 3 cut(s) 152, 3121, 3250
AcuI CTGAAG 8 cut(s) 44, 309, 1278, 1685, 1869, 2078, 2292, 2502
AcyI GRCGYC 2 cut(s) 180, 3589
AjiI CACGTC 1 cut(s) 3232
AjuI GAANNNNNNNTTGG 4 cut(s) 818, 850, 1658, 1690
Alw21I GWGCWC 6 cut(s) 72, 1010, 1281, 1707, 2854, 3376
AlwNI CAGNNNCTG 5 cut(s) 1505, 1922, 2270, 2642, 3202
Ama87I CYCGRG 1 cut(s) 2168
AoxI GGCC 4 cut(s) 1124, 2639, 3014, 3270
ApoI RAATTY 3 cut(s) 152, 3121, 3250
ArsI GACNNNNNNTTYG 4 cut(s) 2030, 2062, 2070, 2102
Asp700I GAANNNNTTC 2 cut(s) 1141, 1677
Asp718I GGTACC 1 cut(s) 3567
AspA2I CCTAGG 1 cut(s) 3017
AspLEI GCGC 4 cut(s) 182, 420, 3444, 3531
AsuC2I CCSGG 2 cut(s) 2169, 2170
AsuII TTCGAA 1 cut(s) 269
AvaI CYCGRG 1 cut(s) 2168
AvaII GGWCC 9 cut(s) 716, 1029, 1055, 1112, 1614, 1913, 2947, 3131, 3310
AvrII CCTAGG 1 cut(s) 3017
AxyI CCTNAGG 1 cut(s) 1180
BaeGI GKGCMC 2 cut(s) 2401, 3181
BamHI GGATCC 1 cut(s) 2794
BanI GGYRCC 2 cut(s) 179, 3567
BanII GRGCYC 3 cut(s) 72, 1010, 1281
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 282, 429
Bbv12I GWGCWC 6 cut(s) 72, 1010, 1281, 1707, 2854, 3376
BbvI GCAGC 9 cut(s) 1372, 1394, 1444, 1447, 1807, 2074, 2218, 2243, 2856
BceAI ACGGC 2 cut(s) 193, 295
BclI TGATCA 3 cut(s) 967, 2238, 3040
BcnI CCSGG 2 cut(s) 2169, 2170
BfmI CTRYAG 2 cut(s) 1830, 2249
BfoI RGCGCY 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 3272
BglII AGATCT 1 cut(s) 2967
BlnI CCTAGG 1 cut(s) 3017
Bme18I GGWCC 9 cut(s) 716, 1029, 1055, 1112, 1614, 1913, 2947, 3131, 3310
BmeT110I CYCGRG 1 cut(s) 2168
BmgBI CACGTC 1 cut(s) 3232
BmiI GGNNCC 5 cut(s) 181, 2796, 3312, 3486, 3569
BoxI GACNNNNGTC 1 cut(s) 18
BpiI GAAGAC 2 cut(s) 282, 429
BpmI CTGGAG 1 cut(s) 392
Bpu10I CCTNAGC 1 cut(s) 879
Bpu14I TTCGAA 1 cut(s) 269
BpuEI CTTGAG 2 cut(s) 1289, 3190
BpuMI CCSGG 2 cut(s) 2169, 2170
Bsa29I ATCGAT 1 cut(s) 622
BsaBI GATNNNNATC 4 cut(s) 486, 492, 966, 1473
BsaHI GRCGYC 2 cut(s) 180, 3589
BsaI GGTCTC 4 cut(s) 55, 368, 2071, 3069
Bse118I RCCGGY 2 cut(s) 392, 1122
Bse21I CCTNAGG 1 cut(s) 1180
Bse3DI GCAATG 3 cut(s) 2689, 2979, 2991
Bse8I GATNNNNATC 4 cut(s) 486, 492, 966, 1473
BseCI ATCGAT 1 cut(s) 622
BseJI GATNNNNATC 4 cut(s) 486, 492, 966, 1473
BseMI GCAATG 3 cut(s) 2689, 2979, 2991
BseMII CTCAG 9 cut(s) 1023, 1140, 1194, 1557, 2361, 2658, 2889, 3141, 3194
BseRI GAGGAG 4 cut(s) 416, 2765, 2876, 3187
BseSI GKGCMC 2 cut(s) 2401, 3181
BseXI GCAGC 9 cut(s) 1372, 1394, 1444, 1447, 1807, 2074, 2218, 2243, 2856
BseYI CCCAGC 1 cut(s) 2618
BsgI GTGCAG 4 cut(s) 1371, 1383, 1401, 3261
Bsh1236I CGCG 2 cut(s) 420, 3444
BshFI GGCC 4 cut(s) 1126, 2641, 3016, 3272
BshNI GGYRCC 2 cut(s) 179, 3567
BshVI ATCGAT 1 cut(s) 622
BsiHKAI GWGCWC 6 cut(s) 72, 1010, 1281, 1707, 2854, 3376
BsiHKCI CYCGRG 1 cut(s) 2168
BsiSI CCGG 5 cut(s) 191, 393, 1123, 2169, 2628
BslFI GGGAC 4 cut(s) 107, 1464, 2677, 3145
BsmBI CGTCTC 4 cut(s) 134, 552, 1044, 2663
BsmFI GGGAC 4 cut(s) 107, 1464, 2677, 3145
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 4 cut(s) 1126, 2641, 3016, 3272
Bso31I GGTCTC 4 cut(s) 55, 368, 2071, 3069
BsoBI CYCGRG 1 cut(s) 2168
Bsp119I TTCGAA 1 cut(s) 269
Bsp1286I GDGCHC 8 cut(s) 72, 1010, 1281, 1707, 2401, 2854, 3181, 3376
Bsp1407I TGTACA 2 cut(s) 1799, 2838
Bsp19I CCATGG 3 cut(s) 2826, 3134, 3295
BspACI CCGC 6 cut(s) 5, 107, 895, 1769, 2680, 2906
BspANI GGCC 4 cut(s) 1126, 2641, 3016, 3272
BspCNI CTCAG 9 cut(s) 1022, 1141, 1193, 1558, 2362, 2659, 2890, 3140, 3195
BspDI ATCGAT 1 cut(s) 622
BspFNI CGCG 2 cut(s) 420, 3444
BspHI TCATGA 1 cut(s) 2017
BspLI GGNNCC 5 cut(s) 181, 2796, 3312, 3486, 3569
BspMI ACCTGC 1 cut(s) 3272
BspQI GCTCTTC 2 cut(s) 998, 1257
BspT104I TTCGAA 1 cut(s) 269
BspT107I GGYRCC 2 cut(s) 179, 3567
BspTNI GGTCTC 4 cut(s) 55, 368, 2071, 3069
BsrDI GCAATG 3 cut(s) 2689, 2979, 2991
BsrFI RCCGGY 2 cut(s) 392, 1122
BsrGI TGTACA 2 cut(s) 1799, 2838
BssAI RCCGGY 2 cut(s) 392, 1122
BssNI GRCGYC 2 cut(s) 180, 3589
BssT1I CCWWGG 5 cut(s) 2826, 3017, 3134, 3295, 3488
BstACI GRCGYC 2 cut(s) 180, 3589
BstAUI TGTACA 2 cut(s) 1799, 2838
BstBI TTCGAA 1 cut(s) 269
BstC8I GCNNGC 6 cut(s) 1124, 1296, 1649, 2323, 2625, 3533
BstDSI CCRYGG 3 cut(s) 2826, 3134, 3295
BstENI CCTNNNNNAGG 1 cut(s) 3172
BstFNI CGCG 2 cut(s) 420, 3444
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 4 cut(s) 182, 420, 3444, 3531
BstMWI GCNNNNNNNGC 6 cut(s) 76, 1432, 2071, 2624, 3113, 3278
BstNSI RCATGY 2 cut(s) 2325, 3442
BstPAI GACNNNNGTC 1 cut(s) 18
BstSFI CTRYAG 2 cut(s) 1830, 2249
BstSLI GKGCMC 2 cut(s) 2401, 3181
BstUI CGCG 2 cut(s) 420, 3444
BstV1I GCAGC 9 cut(s) 1372, 1394, 1444, 1447, 1807, 2074, 2218, 2243, 2856
BstV2I GAAGAC 2 cut(s) 282, 429
BstX2I RGATCY 6 cut(s) 1468, 1489, 2253, 2794, 2967, 3508
BstXI CCANNNNNNTGG 2 cut(s) 1843, 3472
BstYI RGATCY 6 cut(s) 1468, 1489, 2253, 2794, 2967, 3508
Bsu15I ATCGAT 1 cut(s) 622
Bsu36I CCTNAGG 1 cut(s) 1180
BsuRI GGCC 4 cut(s) 1126, 2641, 3016, 3272
BsuTUI ATCGAT 1 cut(s) 622
BtgI CCRYGG 3 cut(s) 2826, 3134, 3295
BtgZI GCGATG 1 cut(s) 2472
BtrI CACGTC 1 cut(s) 3232
BtsI GCAGTG 2 cut(s) 1692, 3376
BveI ACCTGC 1 cut(s) 3272
Cac8I GCNNGC 6 cut(s) 1124, 1296, 1649, 2323, 2625, 3533
CaiI CAGNNNCTG 5 cut(s) 1505, 1922, 2270, 2642, 3202
CciI TCATGA 1 cut(s) 2017
CfoI GCGC 4 cut(s) 182, 420, 3444, 3531
Cfr10I RCCGGY 2 cut(s) 392, 1122
Cfr9I CCCGGG 1 cut(s) 2168
ClaI ATCGAT 1 cut(s) 622
CseI GACGC 4 cut(s) 2640, 3524, 3578, 3590
CsiI ACCWGGT 2 cut(s) 753, 1316
DinI GGCGCC 1 cut(s) 181
DrdI GACNNNNNNGTC 2 cut(s) 200, 1036
DseDI GACNNNNNNGTC 2 cut(s) 200, 1036
EaeI YGGCCR 1 cut(s) 1124
EciI GGCGGA 1 cut(s) 122
Ecl136II GAGCTC 3 cut(s) 70, 1008, 1279
Eco130I CCWWGG 5 cut(s) 2826, 3017, 3134, 3295, 3488
Eco147I AGGCCT 1 cut(s) 3016
Eco24I GRGCYC 3 cut(s) 72, 1010, 1281
Eco31I GGTCTC 4 cut(s) 55, 368, 2071, 3069
Eco47I GGWCC 9 cut(s) 716, 1029, 1055, 1112, 1614, 1913, 2947, 3131, 3310
Eco53kI GAGCTC 3 cut(s) 70, 1008, 1279
Eco57I CTGAAG 8 cut(s) 44, 309, 1278, 1685, 1869, 2078, 2292, 2502
Eco81I CCTNAGG 1 cut(s) 1180
Eco88I CYCGRG 1 cut(s) 2168
EcoICRI GAGCTC 3 cut(s) 70, 1008, 1279
EcoNI CCTNNNNNAGG 1 cut(s) 3172
EcoO109I RGGNCCY 2 cut(s) 2639, 3310
EcoRI GAATTC 1 cut(s) 152
EcoT14I CCWWGG 5 cut(s) 2826, 3017, 3134, 3295, 3488
EcoT38I GRGCYC 3 cut(s) 72, 1010, 1281
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
ErhI CCWWGG 5 cut(s) 2826, 3017, 3134, 3295, 3488
Esp3I CGTCTC 4 cut(s) 134, 552, 1044, 2663
FalI AAGNNNNNCTT 2 cut(s) 1251, 1283
FaqI GGGAC 4 cut(s) 107, 1464, 2677, 3145
FauI CCCGC 2 cut(s) 888, 1762
FbaI TGATCA 3 cut(s) 967, 2238, 3040
FblI GTMKAC 1 cut(s) 3459
FriOI GRGCYC 3 cut(s) 72, 1010, 1281
GlaI GCGC 4 cut(s) 181, 419, 3443, 3530
GsaI CCCAGC 1 cut(s) 2622
GsuI CTGGAG 1 cut(s) 392
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 4 cut(s) 1126, 2641, 3016, 3272
HapII CCGG 5 cut(s) 191, 393, 1123, 2169, 2628
HgaI GACGC 4 cut(s) 2640, 3524, 3578, 3590
HhaI GCGC 4 cut(s) 182, 420, 3444, 3531
Hin1I GRCGYC 2 cut(s) 180, 3589
Hin6I GCGC 4 cut(s) 180, 418, 3442, 3529
HinP1I GCGC 4 cut(s) 180, 418, 3442, 3529
HincII GTYRAC 1 cut(s) 3460
HindII GTYRAC 1 cut(s) 3460
HinfI GANTC 9 cut(s) 342, 575, 1357, 1375, 1454, 1474, 1696, 2570, 2989
HpaII CCGG 5 cut(s) 191, 393, 1123, 2169, 2628
Hpy166II GTNNAC 6 cut(s) 148, 209, 615, 1802, 2947, 3460
Hpy8I GTNNAC 6 cut(s) 148, 209, 615, 1802, 2947, 3460
Hpy99I CGWCG 5 cut(s) 17, 20, 1712, 3461, 3606
HpyCH4IV ACGT 3 cut(s) 1826, 2040, 3231
HpyF10VI GCNNNNNNNGC 6 cut(s) 76, 1432, 2071, 2624, 3113, 3278
HpySE526I ACGT 3 cut(s) 1826, 2040, 3231
Hsp92I GRCGYC 2 cut(s) 180, 3589
HspAI GCGC 4 cut(s) 180, 418, 3442, 3529
KasI GGCGCC 1 cut(s) 179
KpnI GGTACC 1 cut(s) 3571
KroI GCCGGC 1 cut(s) 1122
KroNI GCCGGC 1 cut(s) 1124
Ksp22I TGATCA 3 cut(s) 967, 2238, 3040
LguI GCTCTTC 2 cut(s) 998, 1257
LmnI GCTCC 6 cut(s) 132, 1253, 1411, 1528, 2849, 3490
Lsp1109I GCAGC 9 cut(s) 1372, 1394, 1444, 1447, 1807, 2074, 2218, 2243, 2856
MabI ACCWGGT 2 cut(s) 753, 1316
MaeII ACGT 3 cut(s) 1826, 2040, 3231
MaeIII GTNAC 8 cut(s) 1060, 1195, 1747, 1772, 2098, 2150, 2276, 2651
MflI RGATCY 6 cut(s) 1468, 1489, 2253, 2794, 2967, 3508
MhlI GDGCHC 8 cut(s) 72, 1010, 1281, 1707, 2401, 2854, 3181, 3376
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 2 cut(s) 351, 1384
MmeI TCCRAC 3 cut(s) 12, 506, 1431
MroNI GCCGGC 1 cut(s) 1122
MroXI GAANNNNTTC 2 cut(s) 1141, 1677
MseI TTAA 7 cut(s) 309, 809, 1172, 1353, 1407, 2261, 2375
MslI CAYNNNNRTG 1 cut(s) 2984
MspA1I CMGCKG 1 cut(s) 1385
MspI CCGG 5 cut(s) 191, 393, 1123, 2169, 2628
Mva1269I GAATGC 1 cut(s) 973
MvnI CGCG 2 cut(s) 420, 3444
MwoI GCNNNNNNNGC 6 cut(s) 76, 1432, 2071, 2624, 3113, 3278
NaeI GCCGGC 1 cut(s) 1124
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 2 cut(s) 2169, 2170
NcoI CCATGG 3 cut(s) 2826, 3134, 3295
NgoMIV GCCGGC 1 cut(s) 1122
NlaIV GGNNCC 5 cut(s) 181, 2796, 3312, 3486, 3569
NmeAIII GCCGAG 2 cut(s) 208, 841
NmuCI GTSAC 4 cut(s) 1060, 1195, 2098, 2276
NspI RCATGY 2 cut(s) 2325, 3442
NspV TTCGAA 1 cut(s) 269
PaeI GCATGC 1 cut(s) 2325
PagI TCATGA 1 cut(s) 2017
PasI CCCWGGG 1 cut(s) 1482
PceI AGGCCT 1 cut(s) 3016
PciSI GCTCTTC 2 cut(s) 998, 1257
PcsI WCGNNNNNNNCGW 1 cut(s) 2620
PctI GAATGC 1 cut(s) 973
PdiI GCCGGC 1 cut(s) 1124
PdmI GAANNNNTTC 2 cut(s) 1141, 1677
PfeI GAWTC 7 cut(s) 575, 1357, 1454, 1474, 1696, 2570, 2989
PflFI GACNNNGTC 1 cut(s) 3233
PflMI CCANNNNNTGG 2 cut(s) 1505, 3415
PfoI TCCNGGA 1 cut(s) 2524
PleI GAGTC 2 cut(s) 350, 1383
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 2 cut(s) 350, 1383
PpuMI RGGWCCY 1 cut(s) 3310
PshAI GACNNNNGTC 1 cut(s) 18
PsiI TTATAA 1 cut(s) 2550
Psp124BI GAGCTC 3 cut(s) 72, 1010, 1281
Psp5II RGGWCCY 1 cut(s) 3310
PspFI CCCAGC 1 cut(s) 2618
PspN4I GGNNCC 5 cut(s) 181, 2796, 3312, 3486, 3569
PspPPI RGGWCCY 1 cut(s) 3310
PstNI CAGNNNCTG 5 cut(s) 1505, 1922, 2270, 2642, 3202
PsuI RGATCY 6 cut(s) 1468, 1489, 2253, 2794, 2967, 3508
PsyI GACNNNGTC 1 cut(s) 3233
PvuII CAGCTG 1 cut(s) 1385
RseI CAYNNNNRTG 1 cut(s) 2984
SacI GAGCTC 3 cut(s) 72, 1010, 1281
SalI GTCGAC 1 cut(s) 3458
SapI GCTCTTC 2 cut(s) 998, 1257
SaqAI TTAA 7 cut(s) 309, 809, 1172, 1353, 1407, 2261, 2375
SchI GAGTC 2 cut(s) 351, 1384
SduI GDGCHC 8 cut(s) 72, 1010, 1281, 1707, 2401, 2854, 3181, 3376
SexAI ACCWGGT 2 cut(s) 753, 1316
SfcI CTRYAG 2 cut(s) 1830, 2249
SfoI GGCGCC 1 cut(s) 181
SfuI TTCGAA 1 cut(s) 269
SinI GGWCC 9 cut(s) 716, 1029, 1055, 1112, 1614, 1913, 2947, 3131, 3310
SmaI CCCGGG 1 cut(s) 2170
SmiMI CAYNNNNRTG 1 cut(s) 2984
SmlI CTYRAG 2 cut(s) 1268, 3169
SmoI CTYRAG 2 cut(s) 1268, 3169
SphI GCATGC 1 cut(s) 2325
SseBI AGGCCT 1 cut(s) 3016
SsiI CCGC 6 cut(s) 5, 107, 895, 1769, 2680, 2906
SspDI GGCGCC 1 cut(s) 179
SspI AATATT 1 cut(s) 2476
SstI GAGCTC 3 cut(s) 72, 1010, 1281
StuI AGGCCT 1 cut(s) 3016
StyI CCWWGG 5 cut(s) 2826, 3017, 3134, 3295, 3488
TaiI ACGT 3 cut(s) 1829, 2043, 3234
TaqI TCGA 8 cut(s) 72, 156, 269, 490, 622, 2187, 3339, 3459
TaqII GACCGA 1 cut(s) 1955
TatI WGTACW 4 cut(s) 1799, 1932, 2838, 3435
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 7 cut(s) 575, 1357, 1454, 1474, 1696, 2570, 2989
Tru1I TTAA 7 cut(s) 309, 809, 1172, 1353, 1407, 2261, 2375
Tru9I TTAA 7 cut(s) 309, 809, 1172, 1353, 1407, 2261, 2375
TseFI GTSAC 4 cut(s) 1060, 1195, 2098, 2276
Tsp45I GTSAC 4 cut(s) 1060, 1195, 2098, 2276
TspDTI ATGAA 8 cut(s) 103, 693, 830, 2025, 2360, 2700, 2765, 3588
TspGWI ACGGA 5 cut(s) 145, 194, 1068, 1214, 2051
TspMI CCCGGG 1 cut(s) 2168
Tth111I GACNNNGTC 1 cut(s) 3233
Van91I CCANNNNNTGG 2 cut(s) 1505, 3415
VpaK11BI GGWCC 9 cut(s) 716, 1029, 1055, 1112, 1614, 1913, 2947, 3131, 3310
XagI CCTNNNNNAGG 1 cut(s) 3172
XapI RAATTY 3 cut(s) 152, 3121, 3250
XceI RCATGY 2 cut(s) 2325, 3442
XcmI CCANNNNNNNNNTGG 1 cut(s) 1324
XmaI CCCGGG 1 cut(s) 2168
XmaJI CCTAGG 1 cut(s) 3017
XmiI GTMKAC 1 cut(s) 3459
XmnI GAANNNNTTC 2 cut(s) 1141, 1677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.