RchiOBHm_Chr4g0401121

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000671
Physical Location & Seq
Reverse (-)
19110887 .. 19113352
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37307

Sequence Viewer

Length: 2232 bp
ATGGCCGTGTCTGAGCAAGACAGTGTTTTCGACAAGCTTGCATACCTTTCTCTGGTCTCCAGGATTTGCATAGAGCTTGAAAATCATGATGGGGGTCTTGGAGACAAAGTCTTGGCAAAGTTGATAGCCGACATGGGACGAAACTGTGAGGCAGTGGACGAGTTCCATAAAGAGTTGAAGCAAAACGGTATTGACCTGCCTGAAAATGTCATGTCTATTTTGTTAGCAATTATTCACTCTGTTCTTCCTCCTACAAAGGTTTCTGAGAAGAACAAAATCCCAGACATGGCAATTAACAATAGCATGCAAAGGCATCGAGATGATAAAAGAGGTCAAAGTGAGCTTAGGCGTAGAAGGAGGCATAGAAATTCACAGCAGAATAGGAACTTGCCAATATACAAGTTGAAGAATGAGCTTATTCAAGCTGTGCATGAAAATCAGGTTCTTGTTGTCATCGGTGAGACTGGTTCGGGCAAGACAACTCAGGTAACACAGTATCTTGCTGAAGCAGGTTACACCACAAAGGGCAAGATTGGATGTACTCAACCCCGTAGAGTTGCGGTGACGTCTGTGGCCAAGAGGGTTGCTGAAGAATTTGGTTGTGGATTAGGGGAGGAAGTGGGGTATGCTATTCGTTTTGAAGATTGTACTGGACCAGAAACTGTGATCAAGTACATAACTGATGGGATGCTCTTTAGGGAAATTTTGGTTGATAATGATCTTTCTCAGTACTCTGTGATCATGCTTGATGAAGCTCATGAAAGAACAATCTACACTGATGTCCTCTTTGGATTGCTGAAGCAACTAGTTAAACGACGTCCTGATCTTCGCTTGATTGTCACCTCTGCCACATTGGATGCTGAGAAGTTTTCGGCTTATTTCTTTGACTGTAAGATATTTACTATTCACTTGACTGAACCTGAGGGTGACATACTTCTCTTCTTGACTGGTGAGGAGGAGATTGAATCTGCCTGTCAGTCTCTGAATGAGAAGATGAAAGAATATGGCAAAGGCGTTCCAGAATTGATCATTCTACCGGCTTATGGTGCCCAACCTAGTGAAGAACAGGCAAAGATATTTAAGCCTACTCCTCCAGGGAAGAGAAAAGTGGTGGTGGCTACCAACATTGCTGAAGCTTCTTTAACCATTGATGGGATATTTTATGTCATTGATTCAGGGTATGCAAAGCAAAATGTGTATAATCCGAAGAAAGGGATTGATATGCTGACTGTAACTCCAATTTCACAAGCATCAGCCAAGCAACGAGCTGGACGTGCTGGGCGGACAGGACCAGGCAACTGTTACCGCCTTTACACTGAGGCTGCATTTAAGAATGAGATGTCGCCTACTACAGTTCCAGAAATCCAGAGAACAAATCTTGTATATACCACGCTTATGATGAAAGCAATGGGGATAAACGAGGTTCTATCTTTTGACTTCATGGATCCACCGCCTTCCCAAGCGCTTATTTCTGCTTCTAAACAACTGCAAAATCTAGGAGCATTGGATGCAGAGGGGTCGGTGACAAAATTGGGTAGTAGAATGGCAGTGTTGCCTCTAGATCCACCTTTGTCGAAGATGCTGCTTGCCAGTGTGAACCTTGGATGCAGTGAGGAGATTTTGACCATCATTGCAATGGTTGAGGCTGGCAATATCTTCTACAGGCCTAGAGGAAAACAAGCTGAAGCTGATCAGAAAAGGGCCAGGTTCTTACAACCTGAGGGAGACCATCTGACTTTACTTGCTGTTTATGAGGCATGGAAAGCTAAGAATTTCTCTGTACCCTGGTGTTCTGAGAACTTTGTCCAGTTTCGGTCACTGAGCAAGGCTCGGGATATCAGGAGACAGCTTCTTACTGTCATGGACAAGCATGAATTAGAGGTTGTAAGTGCAGGAAGAGATCGCACAATGATCAGAAAGGCGATTGTAGCTGGATTCTTCTTCCAAGTAGCTAAAAGGGATCCACACGAGGGTTATAGAACCTTTGTTGATAATCAGCTAGTTTTTATCCACCCGAGCAGTGTTCTTTTCCGGAGACAACCAGATTGGGTCATCTACAATGAGCTGGTGATGACCACAAAGGAGTACATGCGCGAGATCAGTGTAATAGAATCTAAATGGCTCATGGAACTGGTGCCGGGATTTTTTAAGGTAACAATTCCTTCAAGGATGAGCAAGCGGAAACGTCAAGAAATTGAACCATTTAGTAGACAGTGTGCTTCGTATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

743

Amino Acids

83.73

Weight (kDa)

8.41

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 137 - 286 2.3e-07 DEAD/DEAH box helicase
AAA_22 PF13401 145 - 278 8.4e-06 AAA domain
Helicase_C PF00271 304 - 430 2.5e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 494 - 520 5.1e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 521 - 580 4e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 638 - 713 1.2e-25 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 569, 820
Acc36I ACCTGC 2 cut(s) 204, 500
AccB1I GGYRCC 2 cut(s) 1046, 2134
AccI GTMKAC 1 cut(s) 2209
AccII CGCG 1 cut(s) 2094
AccIII TCCGGA 1 cut(s) 2031
AciI CCGC 5 cut(s) 560, 1282, 1306, 1451, 2179
AclWI GGATC 5 cut(s) 1439, 1452, 1556, 1955, 1968
AcoI YGGCCR 2 cut(s) 3, 573
AcsI RAATTY 4 cut(s) 367, 593, 702, 1771
AcuI CTGAAG 5 cut(s) 525, 609, 818, 1152, 1704
AcyI GRCGYC 2 cut(s) 566, 817
AfaI GTAC 6 cut(s) 541, 649, 674, 731, 1782, 2087
AfeI AGCGCT 1 cut(s) 1464
AfiI CCNNNNNNNGG 6 cut(s) 52, 286, 1043, 1152, 1211, 1970
AgsI TTSAA 8 cut(s) 80, 178, 406, 422, 641, 965, 2166, 2198
AhlI ACTAGT 1 cut(s) 805
AjiI CACGTC 1 cut(s) 1274
AjnI CCWGG 5 cut(s) 59, 1093, 1291, 1703, 1784
Alw26I GTCTC 7 cut(s) 61, 96, 455, 984, 1719, 1837, 2029
AlwI GGATC 5 cut(s) 1439, 1452, 1556, 1955, 1968
AlwNI CAGNNNCTG 2 cut(s) 662, 982
Ama87I CYCGRG 2 cut(s) 1830, 2014
Aor13HI TCCGGA 1 cut(s) 2031
Aor51HI AGCGCT 1 cut(s) 1464
AoxI GGCC 4 cut(s) 3, 573, 1664, 1701
ApeKI GCWGC 2 cut(s) 1322, 1582
ApoI RAATTY 4 cut(s) 367, 593, 702, 1771
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AspLEI GCGC 2 cut(s) 1465, 2094
AspS9I GGNCC 3 cut(s) 653, 1289, 1701
AsuC2I CCSGG 1 cut(s) 2139
AsuHPI GGTGA 7 cut(s) 470, 574, 832, 938, 962, 1534, 2080
AvaI CYCGRG 2 cut(s) 1830, 2014
AvaII GGWCC 2 cut(s) 653, 1289
AxyI CCTNAGG 2 cut(s) 921, 1719
BaeGI GKGCMC 1 cut(s) 1051
BalI TGGCCA 1 cut(s) 575
BamHI GGATCC 2 cut(s) 1444, 1960
BanI GGYRCC 2 cut(s) 1046, 2134
BauI CACGAG 1 cut(s) 1967
BbvI GCAGC 2 cut(s) 1309, 1569
BccI CCATC 5 cut(s) 83, 677, 1145, 1634, 1737
BcgI CGANNNNNNTGC 8 cut(s) 20, 54, 296, 330, 1500, 1534, 1564, 1598
BciT130I CCWGG 5 cut(s) 61, 1095, 1293, 1705, 1786
BclI TGATCA 5 cut(s) 666, 738, 1026, 1690, 1911
BcnI CCSGG 1 cut(s) 2139
BcoDI GTCTC 7 cut(s) 61, 96, 455, 984, 1719, 1837, 2029
BcuI ACTAGT 1 cut(s) 805
BfaI CTAG 6 cut(s) 806, 1056, 1496, 1559, 1668, 2000
BfmI CTRYAG 2 cut(s) 1350, 1660
BfoI RGCGCY 1 cut(s) 1466
BfuAI ACCTGC 2 cut(s) 204, 500
BisI GCNGC 2 cut(s) 1323, 1583
BlsI GCNGC 2 cut(s) 1324, 1584
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 6 cut(s) 61, 1095, 1293, 1705, 1786, 2139
Bme18I GGWCC 2 cut(s) 653, 1289
BmeT110I CYCGRG 2 cut(s) 1830, 2014
BmgBI CACGTC 1 cut(s) 1274
BmgT120I GGNCC 3 cut(s) 653, 1289, 1701
BmiI GGNNCC 4 cut(s) 1048, 1446, 1962, 2136
BmrFI CCNGG 6 cut(s) 61, 1095, 1293, 1705, 1786, 2139
BmsI GCATC 7 cut(s) 322, 678, 847, 1259, 1498, 1569, 1595
BplI GAGNNNNNCTC 2 cut(s) 1813, 1845
BpmI CTGGAG 2 cut(s) 43, 1077
Bpu10I CCTNAGC 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 2139
BsaBI GATNNNNATC 1 cut(s) 717
BsaHI GRCGYC 2 cut(s) 566, 817
BsaI GGTCTC 2 cut(s) 61, 1719
BsaJI CCNNGG 3 cut(s) 1094, 1600, 1784
BsaWI WCCGGW 1 cut(s) 2031
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 1219, 1249
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 286, 1043, 1152, 1211, 1970
Bse118I RCCGGY 1 cut(s) 1036
Bse1I ACTGG 6 cut(s) 469, 655, 952, 1590, 1807, 2136
Bse21I CCTNAGG 2 cut(s) 921, 1719
Bse3DI GCAATG 4 cut(s) 1125, 1413, 1629, 1641
Bse8I GATNNNNATC 1 cut(s) 717
BseAI TCCGGA 1 cut(s) 2031
BseBI CCWGG 5 cut(s) 61, 1095, 1293, 1705, 1786
BseDI CCNNGG 3 cut(s) 1094, 1600, 1784
BseGI GGATG 6 cut(s) 542, 693, 862, 1513, 1610, 2175
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 6 cut(s) 52, 286, 1043, 1152, 1211, 1970
BseMI GCAATG 4 cut(s) 1125, 1413, 1629, 1641
BseMII CTCAG 9 cut(s) 255, 497, 740, 852, 912, 1308, 1710, 1785, 1811
BseNI ACTGG 6 cut(s) 469, 655, 952, 1590, 1807, 2136
BseRI GAGGAG 4 cut(s) 968, 971, 1080, 1628
BseSI GKGCMC 1 cut(s) 1051
BseXI GCAGC 2 cut(s) 1309, 1569
BseYI CCCAGC 1 cut(s) 1277
BsgI GTGCAG 1 cut(s) 1911
Bsh1236I CGCG 1 cut(s) 2094
BshFI GGCC 4 cut(s) 5, 575, 1666, 1703
BshNI GGYRCC 2 cut(s) 1046, 2134
BsiHKCI CYCGRG 2 cut(s) 1830, 2014
BsiSI CCGG 3 cut(s) 1037, 2032, 2138
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 6 cut(s) 52, 286, 1043, 1152, 1211, 1970
BsmAI GTCTC 7 cut(s) 61, 96, 455, 984, 1719, 1837, 2029
BsmFI GGGAC 1 cut(s) 150
BsnI GGCC 4 cut(s) 5, 575, 1666, 1703
Bso31I GGTCTC 2 cut(s) 61, 1719
BsoBI CYCGRG 2 cut(s) 1830, 2014
Bsp1286I GDGCHC 1 cut(s) 1051
Bsp13I TCCGGA 1 cut(s) 2031
BspACI CCGC 5 cut(s) 560, 1282, 1306, 1451, 2179
BspANI GGCC 4 cut(s) 5, 575, 1666, 1703
BspEI TCCGGA 1 cut(s) 2031
BspFNI CGCG 1 cut(s) 2094
BspHI TCATGA 2 cut(s) 85, 757
BspLI GGNNCC 4 cut(s) 1048, 1446, 1962, 2136
BspMI ACCTGC 2 cut(s) 204, 500
BspPI GGATC 5 cut(s) 1439, 1452, 1556, 1955, 1968
BspT107I GGYRCC 2 cut(s) 1046, 2134
BspTNI GGTCTC 2 cut(s) 61, 1719
BsrDI GCAATG 4 cut(s) 1125, 1413, 1629, 1641
BsrFI RCCGGY 1 cut(s) 1036
BsrI ACTGG 6 cut(s) 469, 655, 952, 1590, 1807, 2136
BssAI RCCGGY 1 cut(s) 1036
BssECI CCNNGG 3 cut(s) 1094, 1600, 1784
BssNI GRCGYC 2 cut(s) 566, 817
BssSI CACGAG 1 cut(s) 1967
BssT1I CCWWGG 1 cut(s) 1600
Bst2BI CACGAG 1 cut(s) 1967
Bst2UI CCWGG 5 cut(s) 61, 1095, 1293, 1705, 1786
Bst6I CTCTTC 3 cut(s) 944, 1094, 1891
BstACI GRCGYC 2 cut(s) 566, 817
BstAPI GCANNNNNTGC 1 cut(s) 1508
BstC8I GCNNGC 5 cut(s) 39, 305, 1587, 1648, 2177
BstF5I GGATG 6 cut(s) 542, 693, 862, 1513, 1610, 2175
BstFNI CGCG 1 cut(s) 2094
BstH2I RGCGCY 1 cut(s) 1466
BstHHI GCGC 2 cut(s) 1465, 2094
BstMAI GTCTC 7 cut(s) 61, 96, 455, 984, 1719, 1837, 2029
BstMWI GCNNNNNNNGC 5 cut(s) 1046, 1274, 1508, 1763, 1928
BstNI CCWGG 5 cut(s) 61, 1095, 1293, 1705, 1786
BstNSI RCATGY 2 cut(s) 307, 2092
BstSCI CCNGG 6 cut(s) 59, 1093, 1291, 1703, 1784, 2137
BstSFI CTRYAG 2 cut(s) 1350, 1660
BstSLI GKGCMC 1 cut(s) 1051
BstUI CGCG 1 cut(s) 2094
BstV1I GCAGC 2 cut(s) 1309, 1569
BstX2I RGATCY 3 cut(s) 1444, 1561, 1960
BstYI RGATCY 3 cut(s) 1444, 1561, 1960
Bsu36I CCTNAGG 2 cut(s) 921, 1719
BsuRI GGCC 4 cut(s) 5, 575, 1666, 1703
BtrI CACGTC 1 cut(s) 1274
BtsCI GGATG 6 cut(s) 542, 693, 862, 1513, 1610, 2175
BtsI GCAGTG 4 cut(s) 159, 1554, 1615, 2026
BveI ACCTGC 2 cut(s) 204, 500
Cac8I GCNNGC 5 cut(s) 39, 305, 1587, 1648, 2177
CaiI CAGNNNCTG 2 cut(s) 662, 982
CciI TCATGA 2 cut(s) 85, 757
CfoI GCGC 2 cut(s) 1465, 2094
Cfr10I RCCGGY 1 cut(s) 1036
Cfr13I GGNCC 3 cut(s) 653, 1289, 1701
Csp6I GTAC 6 cut(s) 540, 648, 673, 730, 1781, 2086
CviQI GTAC 6 cut(s) 540, 648, 673, 730, 1781, 2086
EaeI YGGCCR 2 cut(s) 3, 573
Eam1104I CTCTTC 3 cut(s) 944, 1094, 1891
EarI CTCTTC 3 cut(s) 944, 1094, 1891
EciI GGCGGA 1 cut(s) 1297
Eco130I CCWWGG 1 cut(s) 1600
Eco147I AGGCCT 1 cut(s) 1666
Eco31I GGTCTC 2 cut(s) 61, 1719
Eco32I GATATC 1 cut(s) 1837
Eco47I GGWCC 2 cut(s) 653, 1289
Eco47III AGCGCT 1 cut(s) 1464
Eco57I CTGAAG 5 cut(s) 525, 609, 818, 1152, 1704
Eco81I CCTNAGG 2 cut(s) 921, 1719
Eco88I CYCGRG 2 cut(s) 1830, 2014
EcoRII CCWGG 5 cut(s) 59, 1093, 1291, 1703, 1784
EcoRV GATATC 1 cut(s) 1837
EcoT14I CCWWGG 1 cut(s) 1600
ErhI CCWWGG 1 cut(s) 1600
FaqI GGGAC 1 cut(s) 150
FbaI TGATCA 5 cut(s) 666, 738, 1026, 1690, 1911
FblI GTMKAC 1 cut(s) 2209
Fnu4HI GCNGC 2 cut(s) 1323, 1583
FokI GGATG 6 cut(s) 549, 700, 869, 1520, 1617, 2182
Fsp4HI GCNGC 2 cut(s) 1323, 1583
FspBI CTAG 6 cut(s) 806, 1056, 1496, 1559, 1668, 2000
GlaI GCGC 2 cut(s) 1464, 2093
GluI GCNGC 2 cut(s) 1323, 1583
GsaI CCCAGC 1 cut(s) 1281
GsuI CTGGAG 2 cut(s) 43, 1077
HaeII RGCGCY 1 cut(s) 1466
HaeIII GGCC 4 cut(s) 5, 575, 1666, 1703
HapII CCGG 3 cut(s) 1037, 2032, 2138
HhaI GCGC 2 cut(s) 1465, 2094
Hin1I GRCGYC 2 cut(s) 566, 817
Hin6I GCGC 2 cut(s) 1463, 2092
HinP1I GCGC 2 cut(s) 1463, 2092
HindIII AAGCTT 2 cut(s) 35, 1134
HinfI GANTC 4 cut(s) 965, 1172, 1935, 2111
HpaII CCGG 3 cut(s) 1037, 2032, 2138
HphI GGTGA 7 cut(s) 470, 574, 832, 938, 962, 1534, 2080
Hpy166II GTNNAC 3 cut(s) 157, 1597, 2210
Hpy188I TCNGA 8 cut(s) 13, 265, 984, 1206, 1695, 1734, 1795, 1916
Hpy8I GTNNAC 3 cut(s) 157, 1597, 2210
Hpy99I CGWCG 1 cut(s) 819
HpyAV CCTTC 3 cut(s) 348, 1464, 2172
HpyCH4IV ACGT 4 cut(s) 566, 817, 1273, 2185
HpyF10VI GCNNNNNNNGC 5 cut(s) 1046, 1274, 1508, 1763, 1928
HpySE526I ACGT 4 cut(s) 566, 817, 1273, 2185
Hsp92I GRCGYC 2 cut(s) 566, 817
HspAI GCGC 2 cut(s) 1463, 2092
Kpn2I TCCGGA 1 cut(s) 2031
Ksp22I TGATCA 5 cut(s) 666, 738, 1026, 1690, 1911
LmnI GCTCC 1 cut(s) 1499
Lsp1109I GCAGC 2 cut(s) 1309, 1569
LweI GCATC 7 cut(s) 322, 678, 847, 1259, 1498, 1569, 1595
MaeI CTAG 6 cut(s) 806, 1056, 1496, 1559, 1668, 2000
MaeII ACGT 4 cut(s) 566, 817, 1273, 2185
MflI RGATCY 3 cut(s) 1444, 1561, 1960
MhlI GDGCHC 1 cut(s) 1051
MlsI TGGCCA 1 cut(s) 575
MluNI TGGCCA 1 cut(s) 575
Mox20I TGGCCA 1 cut(s) 575
MroI TCCGGA 1 cut(s) 2031
MscI TGGCCA 1 cut(s) 575
MseI TTAA 6 cut(s) 294, 810, 1080, 1142, 1329, 2148
MslI CAYNNNNRTG 3 cut(s) 318, 1394, 1634
Msp20I TGGCCA 1 cut(s) 575
MspI CCGG 3 cut(s) 1037, 2032, 2138
MspR9I CCNGG 6 cut(s) 61, 1095, 1293, 1705, 1786, 2139
MvaI CCWGG 5 cut(s) 61, 1095, 1293, 1705, 1786
MvnI CGCG 1 cut(s) 2094
MwoI GCNNNNNNNGC 5 cut(s) 1046, 1274, 1508, 1763, 1928
NciI CCSGG 1 cut(s) 2139
NlaIV GGNNCC 4 cut(s) 1048, 1446, 1962, 2136
NmuCI GTSAC 5 cut(s) 562, 838, 926, 1522, 1815
NspI RCATGY 2 cut(s) 307, 2092
PaeI GCATGC 1 cut(s) 307
PagI TCATGA 2 cut(s) 85, 757
PceI AGGCCT 1 cut(s) 1666
PcsI WCGNNNNNNNCGW 1 cut(s) 1270
PfeI GAWTC 4 cut(s) 965, 1172, 1935, 2111
PflFI GACNNNGTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 59
PkrI GCNGC 2 cut(s) 1324, 1584
Psp6I CCWGG 5 cut(s) 59, 1093, 1291, 1703, 1784
PspFI CCCAGC 1 cut(s) 1277
PspGI CCWGG 5 cut(s) 59, 1093, 1291, 1703, 1784
PspN4I GGNNCC 4 cut(s) 1048, 1446, 1962, 2136
PspPI GGNCC 3 cut(s) 653, 1289, 1701
PstNI CAGNNNCTG 2 cut(s) 662, 982
PsuI RGATCY 3 cut(s) 1444, 1561, 1960
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 6 cut(s) 541, 649, 674, 731, 1782, 2087
RsaNI GTAC 6 cut(s) 540, 648, 673, 730, 1781, 2086
RseI CAYNNNNRTG 3 cut(s) 318, 1394, 1634
SaqAI TTAA 6 cut(s) 294, 810, 1080, 1142, 1329, 2148
SatI GCNGC 2 cut(s) 1323, 1583
Sau96I GGNCC 3 cut(s) 653, 1289, 1701
ScaI AGTACT 1 cut(s) 731
ScrFI CCNGG 6 cut(s) 61, 1095, 1293, 1705, 1786, 2139
SduI GDGCHC 1 cut(s) 1051
SfaNI GCATC 7 cut(s) 322, 678, 847, 1259, 1498, 1569, 1595
SfcI CTRYAG 2 cut(s) 1350, 1660
SinI GGWCC 2 cut(s) 653, 1289
SmiMI CAYNNNNRTG 3 cut(s) 318, 1394, 1634
SpeI ACTAGT 1 cut(s) 805
SphI GCATGC 1 cut(s) 307
SseBI AGGCCT 1 cut(s) 1666
SsiI CCGC 5 cut(s) 560, 1282, 1306, 1451, 2179
SspMI CTAG 6 cut(s) 806, 1056, 1496, 1559, 1668, 2000
StuI AGGCCT 1 cut(s) 1666
StyD4I CCNGG 6 cut(s) 59, 1093, 1291, 1703, 1784, 2137
StyI CCWWGG 1 cut(s) 1600
TaiI ACGT 4 cut(s) 569, 820, 1276, 2188
TaqI TCGA 3 cut(s) 30, 316, 1574
TaqII GACCGA 1 cut(s) 1803
TatI WGTACW 5 cut(s) 539, 647, 672, 729, 2085
TfiI GAWTC 4 cut(s) 965, 1172, 1935, 2111
Tru1I TTAA 6 cut(s) 294, 810, 1080, 1142, 1329, 2148
Tru9I TTAA 6 cut(s) 294, 810, 1080, 1142, 1329, 2148
TseFI GTSAC 5 cut(s) 562, 838, 926, 1522, 1815
TseI GCWGC 2 cut(s) 1322, 1582
Tsp45I GTSAC 5 cut(s) 562, 838, 926, 1522, 1815
TspDTI ATGAA 7 cut(s) 447, 765, 774, 1010, 1415, 1429, 1887
Tth111I GACNNNGTC 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 653, 1289
XapI RAATTY 4 cut(s) 367, 593, 702, 1771
XbaI TCTAGA 1 cut(s) 1558
XceI RCATGY 2 cut(s) 307, 2092
XcmI CCANNNNNNNNNTGG 1 cut(s) 1633
XmiI GTMKAC 1 cut(s) 2209
XspI CTAG 6 cut(s) 806, 1056, 1496, 1559, 1668, 2000
ZraI GACGTC 2 cut(s) 567, 818
ZrmI AGTACT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.