RLG00000009217

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000671
Physical Location & Seq
Forward (+)
48998229 .. 49004525
6297 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009217

Sequence Viewer

Length: 3777 bp
ATGCCCTTACCGGACCCGAGTCACCTTCACAAGGATCCCTCCAACCGCAAGCCAGAGACGTCGCGTCGTCGGCTTCAGCCCACCGTACTCTCATCTTCGGTTGAATTGCTCTCCCCTTTAGGCATGAACTTGAGGCGGTGGGTTATTGTAAAGACGCTTACACCCTTTGTACTCATTTCCTTTATCTGTTTGCTTTCTTTTTGTTGTGTTTCAATATCTCCAAAGATGGCTGCCATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAAGTCTGTTCAGAGCTGGAAACCCATCTAGGGTTTGGGGATAAAGTTCTTGCTGAGTTCATAACTGAGATGGGACGAAACTGTGGGAATGTGGATGAATTTGATGCCAAATTGAAGGAGAACGGTGCCGAGATGCCCGATTACTTTGTCCGCACACTTCTCACTATCATTCATGCAATTCTTCCTCCGAAGCCAAAGTCTGAGAATGACTCAATGATAGAGAGTACTACTGAAGGTAAGAAGACCAAGTTTAAGGCTCTGTCTATTGCTGATAACAGGGATAGGGTAAATGATCTCCAGAGGGAAATTGAGATGGAGACCAAGGAAAAGAAAAATGACCGAGAAGAAGATAGGCGTAGAGGTAGAGGTAGAGATAGAGACAGATATGGGGAAAGAGATCGAGACAGAGATAGAGATAGGGATAGGAGAGAGAGGGGCAGGGATAGATATGACAGAGATGAGAGGCGTAGAGATACACATCATGATTATGATAAGGGAAGGCATACAGACCGATCCAATAAGCACAGAAGGGATGGGTATGAAGAGGATGAAGATGTTGGAGAAGATGGTGGTAACAGGAGAGGAGGTGGGGATCAGCGGAGTGGACTGCACCATTCTGATGAGCCTGAATTGTATCAGGTTTATAAGGGTAGGGTATCAAAAGTCATGGACACGGGTTGCTTTGTTCAGTTCAGTGACTTTAGGGGGAAGGAGGGTTTGGTTCATGTTTCACAGATGGCAACTCGGCGTATTGGTAATGCTAAAGATGTGGTGAAGAGGGATCTAGAGGTCTATGTTAAGGTGATTTCAGTTTCTGGTCAGAAGTTGAGCCTTTCAATGAGGGACGTTGATCAGCATACCGGAAAGGATTTGCTTCCATTGAAGAAGAGCTCAGAGGATGATGCTCATAGGACAAACCCGTCGGTATCAAAGCATGATGGGCCTGTGACAAGGACTGGTCTTTCTGGGATTAGAATTGTGGAAGAGAACAATGTTGCCCCTTCTCGGCGGCCATTAAAGAGAATGAGCTCGCCGGAGAAGTGGGAAGCTAAACAGTTGATTGCCTCAGGTGTTTTGGGTGTTACAGAATATCCAATGTATGATGAGGAAACAGAGGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAGCTTGAAATTGAGCTTAATGAGGACGAGCCAGCCTTTTTGCAAGGGCAAAGCAGGTATTCTGTAGATGTGTCGCCTGTGAAAATTTTTAAGAATCCAGAGGGGTCATTAAGTCGTGCAGCTGCACTTCAATCTGCACTTATTAAAGAGCGCAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAAACTGGTGAGAGGCATCTTGCACAGGAGCTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACTGTCAGTTTTGGGCAGAGGTCAAAGCTCTCACTTCAGGAACAGAGGCAGAGCTTGCCTATCTACAAGCTCAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCGGGCAAGACAACCCAGGTAACACAATATCTTGCAGAAGCTGGTTACACAACAATGGGTAAGATTGGGTGTACACAACCACGTAGGGTGGCCGCGATGTCTGTTGCCAAGAGAGTTGCTGAAGAATTTGGTTGTCGTTTGGGGGAGGAAGTTGGCTATGCTATTCGTTTTGAGGATTGCACTGGCCCTGATACTGTCATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTTTGATCGATGAAAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAGCTTATTAAGCGGAGACCAGACCTACGTTTGATTGTCACATCTGCTACCTTAGATGCTGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATACCAGGGAGAACTTTTCCTGTAGAGATACTATACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTCTAATCACTGTTCTACAGATTCACTTAACAGAACCAGAAGGGGACATCCTTCTCTTCTTGACGGGACAGGAAGAAATTGATTTCTCATGCCAGTCACTTTTTGAGAGGATGAAAGGTCTTGGTAAAAATGTACCTGAGCTGATTATCTTACCAGTTTACAGTGCCCTTCCTAGTGAAATGCAGTCAAGGATTTTTGATCCAGCTCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACTAATATTGCTGAGGCATCACTAACCATTGATGGGATATTTTATGTAATCGATCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAAGGGCTGGATTCACTGGTCATAACCCCAATTTCACAAGCATCAGCCAAGCAAAGAGCTGGGCGCGCTGGGCGTACTGGGCCTGGAAAGTGTTACCGCCTCTACACTGAGAGTGCATACCGCAACGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGTTAACTACACTTACCATGAAAGCAATGGGGATAAATGATCTGTTGTCTTTTGATTTTATGGATCCTCCCTCACCCCAAGCACTCATTTCTGCTATGGAACAACTGTACAGTTTAGGAGCACTCGATGAGGAGGGGCTTCTCACCAAACTGGGTAGGAAAATGGCTGAGTTTCCTCTTGATCCACCACTATCTAAGATGCTACTGGCCAGTGTGGACCTTGGATGCAGTGATGAGATCTTGACCATGATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAACAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGACCATCTGACTTTACTTGCAGTGTATGAGGCTTGGAAAGCTAAGAACTTTTCAGGGCCATGGTGCTTTGAGAACTTTGTTCAGTCTCGATCGCTTAGGAGGGCACAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGATGTTGTGAGTGCTGGAAAGAATTTTACACAGATCAGGAAGGCAATTACGGCGGGATTTTTCTTCCATGGTGCTAGAAAGGACCCTCAGGAGGGGTATAGAACCTTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCGCTCTTCCAGAGACAACCAGATTGGATCATCTACCATGAGTTGGTTATGACTACGAAGGAGTATATGCGTGAGGTAACTGTCGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCAAAGTGGCAGACCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAGCGTATTGAACCGCTATATGACAGATACCATGAACCAAACTCTTGGCGTCTTAGTAAACGACGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1259

Amino Acids

143.38

Weight (kDa)

7.12

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 303 - 373 4.7e-10 S1 RNA binding domain
DEAD PF00270 612 - 761 1.3e-07 DEAD/DEAH box helicase
AAA_22 PF13401 620 - 745 3.2e-06 AAA domain
Helicase_C PF00271 805 - 935 9.5e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 997 - 1025 8.7e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1026 - 1085 3.3e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1143 - 1219 9e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 924, 2715
AasI GACNNNNNNGTC 1 cut(s) 1198
AatII GACGTC 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 1473
AccB1I GGYRCC 1 cut(s) 404
AccB7I CCANNNNNTGG 2 cut(s) 1670, 3580
AccII CGCG 3 cut(s) 64, 1988, 2790
AcoI YGGCCR 3 cut(s) 1289, 1983, 3102
AcsI RAATTY 5 cut(s) 377, 1512, 2018, 2877, 3415
AcuI CTGAAG 5 cut(s) 59, 531, 1778, 2034, 2850
AcyI GRCGYC 2 cut(s) 59, 3754
AhdI GACNNNNNGTC 1 cut(s) 63
AjiI CACGTC 1 cut(s) 3770
AjnI CCWGG 7 cut(s) 1908, 2332, 2608, 2689, 2806, 3198, 3653
AjuI GAANNNNNNNTTGG 2 cut(s) 980, 1012
Alw21I GWGCWC 3 cut(s) 1172, 1310, 3019
Alw26I GTCTC 9 cut(s) 50, 590, 651, 675, 1880, 2236, 3234, 3330, 3544
AlwNI CAGNNNCTG 3 cut(s) 1094, 1670, 1934
Ama87I CYCGRG 2 cut(s) 16, 257
AoxI GGCC 9 cut(s) 1220, 1289, 1716, 1983, 2077, 2804, 3102, 3179, 3296
ApeKI GCWGC 6 cut(s) 230, 1547, 1550, 1597, 1600, 2371
ApoI RAATTY 5 cut(s) 377, 1512, 2018, 2877, 3415
ArsI GACNNNNNNTTYG 2 cut(s) 461, 493
Asp700I GAANNNNTTC 1 cut(s) 1842
AspA2I CCTAGG 1 cut(s) 3182
AspLEI GCGC 5 cut(s) 1581, 2790, 2792, 3541, 3696
AspS9I GGNCC 7 cut(s) 13, 1220, 2078, 2804, 3112, 3296, 3475
AsuHPI GGTGA 7 cut(s) 14, 1063, 1093, 1685, 1895, 2961, 3031
AvaI CYCGRG 2 cut(s) 16, 257
AvaII GGWCC 3 cut(s) 13, 3112, 3475
AvrII CCTAGG 1 cut(s) 3182
AxyI CCTNAGG 2 cut(s) 1345, 3480
BaeGI GKGCMC 2 cut(s) 2566, 3346
BalI TGGCCA 1 cut(s) 3104
BamHI GGATCC 2 cut(s) 34, 2959
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 2 cut(s) 1172, 1310
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
BbsI GAAGAC 2 cut(s) 527, 1763
Bbv12I GWGCWC 3 cut(s) 1172, 1310, 3019
BbvCI CCTCAGC 2 cut(s) 1422, 2646
BbvI GCAGC 6 cut(s) 217, 1537, 1559, 1609, 1612, 2383
BceAI ACGGC 1 cut(s) 3459
BciT130I CCWGG 7 cut(s) 1910, 2334, 2610, 2691, 2808, 3200, 3655
BclI TGATCA 2 cut(s) 1129, 3205
BcoDI GTCTC 9 cut(s) 50, 590, 651, 675, 1880, 2236, 3234, 3330, 3544
BfaI CTAG 6 cut(s) 308, 1064, 2571, 3183, 3468, 3647
BfmI CTRYAG 3 cut(s) 1491, 2349, 2414
BfuAI ACCTGC 1 cut(s) 1473
BglII AGATCT 1 cut(s) 3132
BisI GCNGC 8 cut(s) 231, 1289, 1548, 1551, 1598, 1601, 1986, 2372
BlnI CCTAGG 1 cut(s) 3182
BlsI GCNGC 8 cut(s) 232, 1290, 1549, 1552, 1599, 1602, 1987, 2373
BmcAI AGTACT 2 cut(s) 505, 2156
Bme1390I CCNGG 7 cut(s) 1910, 2334, 2610, 2691, 2808, 3200, 3655
Bme18I GGWCC 3 cut(s) 13, 3112, 3475
BmeRI GACNNNNNGTC 1 cut(s) 63
BmeT110I CYCGRG 2 cut(s) 16, 257
BmgBI CACGTC 1 cut(s) 3770
BmgT120I GGNCC 7 cut(s) 13, 1220, 2078, 2804, 3112, 3296, 3475
BmiI GGNNCC 5 cut(s) 15, 36, 406, 2961, 3477
BmrFI CCNGG 7 cut(s) 1910, 2334, 2610, 2691, 2808, 3200, 3655
BmrI ACTGGG 2 cut(s) 2811, 3056
BmuI ACTGGG 2 cut(s) 2811, 3056
BoxI GACNNNNGTC 1 cut(s) 18
BpiI GAAGAC 2 cut(s) 527, 1763
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 1 cut(s) 560
Bpu10I CCTNAGC 4 cut(s) 1422, 2535, 2646, 3335
BpuEI CTTGAG 2 cut(s) 151, 1814
Bsa29I ATCGAT 2 cut(s) 2136, 2685
BsaAI YACGTR 1 cut(s) 1976
BsaBI GATNNNNATC 3 cut(s) 756, 1638, 2132
BsaHI GRCGYC 2 cut(s) 59, 3754
BsaI GGTCTC 3 cut(s) 590, 2236, 3234
BsaWI WCCGGW 2 cut(s) 10, 1139
BsaXI ACNNNNNCTCC 2 cut(s) 850, 880
Bse21I CCTNAGG 2 cut(s) 1345, 3480
Bse3DI GCAATG 1 cut(s) 2928
Bse8I GATNNNNATC 3 cut(s) 756, 1638, 2132
BseBI CCWGG 7 cut(s) 1910, 2334, 2610, 2691, 2808, 3200, 3655
BseCI ATCGAT 2 cut(s) 2136, 2685
BseJI GATNNNNATC 3 cut(s) 756, 1638, 2132
BseMI GCAATG 1 cut(s) 2928
BsePI GCGCGC 1 cut(s) 2788
BseRI GAGGAG 2 cut(s) 876, 3041
BseSI GKGCMC 2 cut(s) 2566, 3346
BseXI GCAGC 6 cut(s) 217, 1537, 1559, 1609, 1612, 2383
BseYI CCCAGC 2 cut(s) 2783, 2792
BsgI GTGCAG 4 cut(s) 872, 1536, 1548, 1566
Bsh1236I CGCG 3 cut(s) 64, 1988, 2790
Bsh1285I CGRYCG 1 cut(s) 3332
BshFI GGCC 9 cut(s) 1222, 1291, 1718, 1985, 2079, 2806, 3104, 3181, 3298
BshNI GGYRCC 1 cut(s) 404
BshVI ATCGAT 2 cut(s) 2136, 2685
BsiEI CGRYCG 1 cut(s) 3332
BsiHKAI GWGCWC 3 cut(s) 1172, 1310, 3019
BsiHKCI CYCGRG 2 cut(s) 16, 257
BsiSI CCGG 3 cut(s) 11, 1140, 1313
BslFI GGGAC 5 cut(s) 366, 1136, 2456, 2478, 2842
BsmAI GTCTC 9 cut(s) 50, 590, 651, 675, 1880, 2236, 3234, 3330, 3544
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 5 cut(s) 366, 1136, 2456, 2478, 2842
BsnI GGCC 9 cut(s) 1222, 1291, 1718, 1985, 2079, 2806, 3104, 3181, 3298
Bso31I GGTCTC 3 cut(s) 590, 2236, 3234
BsoBI CYCGRG 2 cut(s) 16, 257
Bsp1286I GDGCHC 5 cut(s) 1172, 1310, 2566, 3019, 3346
Bsp1407I TGTACA 2 cut(s) 1964, 3003
Bsp19I CCATGG 2 cut(s) 3299, 3460
BspANI GGCC 9 cut(s) 1222, 1291, 1718, 1985, 2079, 2806, 3104, 3181, 3298
BspDI ATCGAT 2 cut(s) 2136, 2685
BspFNI CGCG 3 cut(s) 64, 1988, 2790
BspHI TCATGA 2 cut(s) 760, 2182
BspLI GGNNCC 5 cut(s) 15, 36, 406, 2961, 3477
BspMI ACCTGC 1 cut(s) 1473
BspQI GCTCTTC 3 cut(s) 1160, 1422, 3548
BspT107I GGYRCC 1 cut(s) 404
BspTNI GGTCTC 3 cut(s) 590, 2236, 3234
BsrDI GCAATG 1 cut(s) 2928
BsrGI TGTACA 2 cut(s) 1964, 3003
BssHII GCGCGC 1 cut(s) 2788
BssNI GRCGYC 2 cut(s) 59, 3754
BssT1I CCWWGG 7 cut(s) 270, 600, 1646, 3115, 3182, 3299, 3460
Bst2UI CCWGG 7 cut(s) 1910, 2334, 2610, 2691, 2808, 3200, 3655
BstACI GRCGYC 2 cut(s) 59, 3754
BstAPI GCANNNNNTGC 1 cut(s) 1813
BstAUI TGTACA 2 cut(s) 1964, 3003
BstBAI YACGTR 1 cut(s) 1976
BstC8I GCNNGC 6 cut(s) 50, 1310, 1461, 1814, 2790, 3698
BstDSI CCRYGG 2 cut(s) 3299, 3460
BstENI CCTNNNNNAGG 1 cut(s) 29
BstFNI CGCG 3 cut(s) 64, 1988, 2790
BstHHI GCGC 5 cut(s) 1581, 2790, 2792, 3541, 3696
BstMAI GTCTC 9 cut(s) 50, 590, 651, 675, 1880, 2236, 3234, 3330, 3544
BstMCI CGRYCG 1 cut(s) 3332
BstNI CCWGG 7 cut(s) 1910, 2334, 2610, 2691, 2808, 3200, 3655
BstNSI RCATGY 1 cut(s) 1729
BstPAI GACNNNNGTC 1 cut(s) 18
BstSCI CCNGG 7 cut(s) 1908, 2332, 2608, 2689, 2806, 3198, 3653
BstSFI CTRYAG 3 cut(s) 1491, 2349, 2414
BstSLI GKGCMC 2 cut(s) 2566, 3346
BstUI CGCG 3 cut(s) 64, 1988, 2790
BstV1I GCAGC 6 cut(s) 217, 1537, 1559, 1609, 1612, 2383
BstV2I GAAGAC 2 cut(s) 527, 1763
BstX2I RGATCY 6 cut(s) 34, 1060, 1633, 1654, 2959, 3132
BstXI CCANNNNNNTGG 1 cut(s) 3750
BstYI RGATCY 6 cut(s) 34, 1060, 1633, 1654, 2959, 3132
Bsu15I ATCGAT 2 cut(s) 2136, 2685
Bsu36I CCTNAGG 2 cut(s) 1345, 3480
BsuRI GGCC 9 cut(s) 1222, 1291, 1718, 1985, 2079, 2806, 3104, 3181, 3298
BsuTUI ATCGAT 2 cut(s) 2136, 2685
BtgI CCRYGG 2 cut(s) 3299, 3460
BtgZI GCGATG 1 cut(s) 2003
BtrI CACGTC 1 cut(s) 3770
BtsI GCAGTG 4 cut(s) 1857, 3130, 3267, 3541
BveI ACCTGC 1 cut(s) 1473
Cac8I GCNNGC 6 cut(s) 50, 1310, 1461, 1814, 2790, 3698
CaiI CAGNNNCTG 3 cut(s) 1094, 1670, 1934
CciI TCATGA 2 cut(s) 760, 2182
CfoI GCGC 5 cut(s) 1581, 2790, 2792, 3541, 3696
Cfr13I GGNCC 7 cut(s) 13, 1220, 2078, 2804, 3112, 3296, 3475
ClaI ATCGAT 2 cut(s) 2136, 2685
CseI GACGC 4 cut(s) 53, 163, 3689, 3743
DrdI GACNNNNNNGTC 1 cut(s) 1198
DriI GACNNNNNGTC 1 cut(s) 63
DseDI GACNNNNNNGTC 1 cut(s) 1198
EaeI YGGCCR 3 cut(s) 1289, 1983, 3102
Eam1105I GACNNNNNGTC 1 cut(s) 63
Ecl136II GAGCTC 2 cut(s) 1170, 1308
Eco130I CCWWGG 7 cut(s) 270, 600, 1646, 3115, 3182, 3299, 3460
Eco147I AGGCCT 1 cut(s) 3181
Eco24I GRGCYC 2 cut(s) 1172, 1310
Eco31I GGTCTC 3 cut(s) 590, 2236, 3234
Eco47I GGWCC 3 cut(s) 13, 3112, 3475
Eco53kI GAGCTC 2 cut(s) 1170, 1308
Eco57I CTGAAG 5 cut(s) 59, 531, 1778, 2034, 2850
Eco81I CCTNAGG 2 cut(s) 1345, 3480
Eco88I CYCGRG 2 cut(s) 16, 257
EcoICRI GAGCTC 2 cut(s) 1170, 1308
EcoNI CCTNNNNNAGG 1 cut(s) 29
EcoO109I RGGNCCY 1 cut(s) 3475
EcoRII CCWGG 7 cut(s) 1908, 2332, 2608, 2689, 2806, 3198, 3653
EcoT14I CCWWGG 7 cut(s) 270, 600, 1646, 3115, 3182, 3299, 3460
EcoT22I ATGCAT 1 cut(s) 2398
EcoT38I GRGCYC 2 cut(s) 1172, 1310
ErhI CCWWGG 7 cut(s) 270, 600, 1646, 3115, 3182, 3299, 3460
Esp3I CGTCTC 1 cut(s) 50
FaqI GGGAC 5 cut(s) 366, 1136, 2456, 2478, 2842
FauI CCCGC 1 cut(s) 3439
FbaI TGATCA 2 cut(s) 1129, 3205
Fnu4HI GCNGC 8 cut(s) 231, 1289, 1548, 1551, 1598, 1601, 1986, 2372
FriOI GRGCYC 2 cut(s) 1172, 1310
Fsp4HI GCNGC 8 cut(s) 231, 1289, 1548, 1551, 1598, 1601, 1986, 2372
FspBI CTAG 6 cut(s) 308, 1064, 2571, 3183, 3468, 3647
GlaI GCGC 5 cut(s) 1580, 2789, 2791, 3540, 3695
GluI GCNGC 8 cut(s) 231, 1289, 1548, 1551, 1598, 1601, 1986, 2372
GsaI CCCAGC 2 cut(s) 2787, 2796
GsuI CTGGAG 1 cut(s) 560
HaeIII GGCC 9 cut(s) 1222, 1291, 1718, 1985, 2079, 2806, 3104, 3181, 3298
HapII CCGG 3 cut(s) 11, 1140, 1313
HgaI GACGC 4 cut(s) 53, 163, 3689, 3743
HhaI GCGC 5 cut(s) 1581, 2790, 2792, 3541, 3696
Hin1I GRCGYC 2 cut(s) 59, 3754
Hin6I GCGC 5 cut(s) 1579, 2788, 2790, 3539, 3694
HinP1I GCGC 5 cut(s) 1579, 2788, 2790, 3539, 3694
HincII GTYRAC 2 cut(s) 2901, 3625
HindII GTYRAC 2 cut(s) 2901, 3625
HindIII AAGCTT 1 cut(s) 2228
HinfI GANTC 8 cut(s) 19, 488, 1522, 1619, 1639, 2419, 2735, 3154
HpaI GTTAAC 1 cut(s) 2901
HpaII CCGG 3 cut(s) 11, 1140, 1313
HphI GGTGA 7 cut(s) 14, 1063, 1093, 1685, 1895, 2961, 3031
Hpy166II GTNNAC 8 cut(s) 884, 1965, 1967, 2557, 2901, 3112, 3625, 3764
Hpy8I GTNNAC 8 cut(s) 884, 1965, 1967, 2557, 2901, 3112, 3625, 3764
Hpy99I CGWCG 5 cut(s) 64, 69, 72, 1204, 3771
HpyCH4IV ACGT 5 cut(s) 59, 1125, 1975, 2254, 3769
HpySE526I ACGT 5 cut(s) 59, 1125, 1975, 2254, 3769
Hsp92I GRCGYC 2 cut(s) 59, 3754
HspAI GCGC 5 cut(s) 1579, 2788, 2790, 3539, 3694
Ksp22I TGATCA 2 cut(s) 1129, 3205
KspAI GTTAAC 1 cut(s) 2901
LguI GCTCTTC 3 cut(s) 1160, 1422, 3548
LmnI GCTCC 4 cut(s) 1418, 1693, 2608, 3014
Lsp1109I GCAGC 6 cut(s) 217, 1537, 1559, 1609, 1612, 2383
MaeI CTAG 6 cut(s) 308, 1064, 2571, 3183, 3468, 3647
MaeII ACGT 5 cut(s) 59, 1125, 1975, 2254, 3769
MflI RGATCY 6 cut(s) 34, 1060, 1633, 1654, 2959, 3132
MhlI GDGCHC 5 cut(s) 1172, 1310, 2566, 3019, 3346
MlsI TGGCCA 1 cut(s) 3104
MluNI TGGCCA 1 cut(s) 3104
MlyI GAGTC 2 cut(s) 28, 482
MmeI TCCRAC 3 cut(s) 66, 817, 1596
Mox20I TGGCCA 1 cut(s) 3104
Mph1103I ATGCAT 1 cut(s) 2398
MroXI GAANNNNTTC 1 cut(s) 1842
MscI TGGCCA 1 cut(s) 3104
MslI CAYNNNNRTG 4 cut(s) 765, 897, 1946, 2202
Msp20I TGGCCA 1 cut(s) 3104
MspA1I CMGCKG 2 cut(s) 877, 1550
MspI CCGG 3 cut(s) 11, 1140, 1313
MspR9I CCNGG 7 cut(s) 1910, 2334, 2610, 2691, 2808, 3200, 3655
MvaI CCWGG 7 cut(s) 1910, 2334, 2610, 2691, 2808, 3200, 3655
MvnI CGCG 3 cut(s) 64, 1988, 2790
NcoI CCATGG 2 cut(s) 3299, 3460
NlaIV GGNNCC 5 cut(s) 15, 36, 406, 2961, 3477
NmeAIII GCCGAG 3 cut(s) 433, 1003, 1264
NmuCI GTSAC 5 cut(s) 20, 974, 1225, 2263, 2493
NsiI ATGCAT 1 cut(s) 2398
NspI RCATGY 1 cut(s) 1729
PaeR7I CTCGAG 1 cut(s) 257
PagI TCATGA 2 cut(s) 760, 2182
PauI GCGCGC 1 cut(s) 2788
PceI AGGCCT 1 cut(s) 3181
PciSI GCTCTTC 3 cut(s) 1160, 1422, 3548
PdmI GAANNNNTTC 1 cut(s) 1842
PfeI GAWTC 6 cut(s) 1522, 1619, 1639, 2419, 2735, 3154
PflMI CCANNNNNTGG 2 cut(s) 1670, 3580
PfoI TCCNGGA 1 cut(s) 2689
PkrI GCNGC 8 cut(s) 232, 1290, 1549, 1552, 1599, 1602, 1987, 2373
Ple19I CGATCG 1 cut(s) 3332
PleI GAGTC 2 cut(s) 27, 482
PpsI GAGTC 2 cut(s) 27, 482
Ppu21I YACGTR 1 cut(s) 1976
PpuMI RGGWCCY 1 cut(s) 3475
PshAI GACNNNNGTC 1 cut(s) 18
PsiI TTATAA 2 cut(s) 924, 2715
Psp124BI GAGCTC 2 cut(s) 1172, 1310
Psp5II RGGWCCY 1 cut(s) 3475
Psp6I CCWGG 7 cut(s) 1908, 2332, 2608, 2689, 2806, 3198, 3653
PspFI CCCAGC 2 cut(s) 2783, 2792
PspGI CCWGG 7 cut(s) 1908, 2332, 2608, 2689, 2806, 3198, 3653
PspN4I GGNNCC 5 cut(s) 15, 36, 406, 2961, 3477
PspPI GGNCC 7 cut(s) 13, 1220, 2078, 2804, 3112, 3296, 3475
PspPPI RGGWCCY 1 cut(s) 3475
PspXI VCTCGAGB 1 cut(s) 257
PstNI CAGNNNCTG 3 cut(s) 1094, 1670, 1934
PsuI RGATCY 6 cut(s) 34, 1060, 1633, 1654, 2959, 3132
PteI GCGCGC 1 cut(s) 2788
PvuI CGATCG 1 cut(s) 3332
PvuII CAGCTG 1 cut(s) 1550
RseI CAYNNNNRTG 4 cut(s) 765, 897, 1946, 2202
SacI GAGCTC 2 cut(s) 1172, 1310
SapI GCTCTTC 3 cut(s) 1160, 1422, 3548
SatI GCNGC 8 cut(s) 231, 1289, 1548, 1551, 1598, 1601, 1986, 2372
Sau96I GGNCC 7 cut(s) 13, 1220, 2078, 2804, 3112, 3296, 3475
ScaI AGTACT 2 cut(s) 505, 2156
SchI GAGTC 2 cut(s) 28, 482
ScrFI CCNGG 7 cut(s) 1910, 2334, 2610, 2691, 2808, 3200, 3655
SduI GDGCHC 5 cut(s) 1172, 1310, 2566, 3019, 3346
SfcI CTRYAG 3 cut(s) 1491, 2349, 2414
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 3 cut(s) 13, 3112, 3475
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 4 cut(s) 765, 897, 1946, 2202
SmlI CTYRAG 3 cut(s) 130, 257, 1829
SmoI CTYRAG 3 cut(s) 130, 257, 1829
SseBI AGGCCT 1 cut(s) 3181
SspI AATATT 1 cut(s) 2641
SspMI CTAG 6 cut(s) 308, 1064, 2571, 3183, 3468, 3647
SstI GAGCTC 2 cut(s) 1172, 1310
StuI AGGCCT 1 cut(s) 3181
StyD4I CCNGG 7 cut(s) 1908, 2332, 2608, 2689, 2806, 3198, 3653
StyI CCWWGG 7 cut(s) 270, 600, 1646, 3115, 3182, 3299, 3460
TaiI ACGT 5 cut(s) 62, 1128, 1978, 2257, 3772
TaqI TCGA 7 cut(s) 258, 679, 2136, 2310, 2685, 3021, 3328
TaqII GACCGA 2 cut(s) 633, 804
TatI WGTACW 6 cut(s) 169, 503, 1964, 2097, 2154, 3003
TauI GCSGC 2 cut(s) 1291, 1988
TfiI GAWTC 6 cut(s) 1522, 1619, 1639, 2419, 2735, 3154
TseFI GTSAC 5 cut(s) 20, 974, 1225, 2263, 2493
TseI GCWGC 6 cut(s) 230, 1547, 1550, 1597, 1600, 2371
Tsp45I GTSAC 5 cut(s) 20, 974, 1225, 2263, 2493
Van91I CCANNNNNTGG 2 cut(s) 1670, 3580
VpaK11BI GGWCC 3 cut(s) 13, 3112, 3475
XagI CCTNNNNNAGG 1 cut(s) 29
XapI RAATTY 5 cut(s) 377, 1512, 2018, 2877, 3415
XbaI TCTAGA 1 cut(s) 1063
XceI RCATGY 1 cut(s) 1729
XcmI CCANNNNNNNNNTGG 3 cut(s) 311, 2920, 3637
XhoI CTCGAG 1 cut(s) 257
XmaJI CCTAGG 1 cut(s) 3182
XmnI GAANNNNTTC 1 cut(s) 1842
XspI CTAG 6 cut(s) 308, 1064, 2571, 3183, 3468, 3647
ZraI GACGTC 1 cut(s) 60
ZrmI AGTACT 2 cut(s) 505, 2156
Zsp2I ATGCAT 1 cut(s) 2398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.