Rroxscaffold_5G00345000

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000671
Physical Location & Seq
Reverse (-)
15223551 .. 15230706
7156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00345000.1

Sequence Viewer

Length: 3567 bp
ATGGCTGCCATTGACGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAAATCTGTTCAGAGCTGGAAACCCATCTAGGGTTTGGGGATAAAGTTCTTGCTGAGTTCATAACTGAGATGGGACGAAACTGTGGGAACGTGGATGAATTTGATGTCAAATTGAAGGAGAACGGTGCCGAGATGCCCGATTACTTTGTCCGCACACTCCTCACTATCATTCATGCAATTCTTCCTCCGAAGCCAAAGTCTGAGAATGACTCAATGATAGAGAGTGCTACCGAAGGTAAGAAGACCAAGTTTAAGGCTCTGTCTATTGCTGATAACAGGGATAGGGTAAATGATCTCCAGAGGGAAATTGAGATGGAGAACAAGGAAAAGAAAAATGACCGAGAAGAGTGGGGTGATGAGCGCGAAGAAGATAGGCATAGAGGTAGAGGTAGAGATAGAGACAGATATGGGGAAAGAGATCGAGACAGAGATAGGGATAGGAGAGCGAGGGGCAGGGATAGGTATGATAGAGATGAGAGGCGTAGAGATACACATCATGATTATGATAAGGGAAGGCATGCAGACCGATCCAATAAGCACAGAAGGGATGGGTATGAAGAGGATGAAGATGTTAGAGACGATGGTGGTAACAGGAGAGGAGGTGGGGATCAGCGGAGTGGACTGCATCATTCTGATGAGCCTGAATTGTATCAGGTTTATAAGGGCAGGGTATCAAAAGTCATGGACACGGGTTGCTTTGTTCAGTTCAGTGACTTTAGGGGGAAGGAGGGTTTGGTTCATGTTTCACAGATGGCAACTCGGCGTATTGGTAATGCTAAAGATGTTGTGAAGAGGGATCAAGAGGTCTATGTTAAGGTGATTTCAGTTTCTGGTCAGAAGTTGAGCCTTTCAATGAGGGATGTCGATCAGCATACCGGAAAGGATTTGCTTCCATTGAAGAAGAGCTCAGAGGATGATGCTCATAGGACAAACCCTTCGGTATCAAAGCATGATGGGCCTGTGACAAGGACTGGTCTTTCTGGGATTAGAATTGTGGAAGAGAACAATGTTGCCCCTTCTCGGCGGCCATTAAAGAGAATGAGCTCACCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCAGGTGTTTTGGGTGTTACAGAATATCCAATGTATGATGAGGAAACAGAGGGGGTGCTTTATGAAGAAGAGGGAGCTGAGGAAGAGCTTGAAATTGAGCTTAATGAGGACGAGCCAGCCTTTTTGCAAGGGCAAAGCAGGTATTCTGTAGATATGTCACCTGTGAAAATCTTTAAGAATCCAGAGGGGTCATTAAGTCGTGCAGCTGCACTTCAATCTGCACTTATTAAAGAGCGCAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAGCTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACTGTCAGTTTTGGGCAGAGGTCAAAGCTCTCACTTCAGGAACAGAGGCAGAGCTTGCCTATCTACAAGCTCAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCAGGCAAGACAACCCAGGTAACACAATATCTTGCAGAAGCAGGTTACACAACAATGGGTAAGATTGGGTGTACACAACCACGTAGGGTGGCCGCGATGTCTGTAGCCAAGAGAGTTGCTGAAGAATTTGGTTGTCGTTTGGGGGAGGAAGTTGGCTATGCTATTCGTTTTGAGGATTGCACTGGCCCTGATACTGTCATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTTTGATTGACGAGAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAACTTATCAAGCGGAGACCAGACCTACGTTTGATTGTCACATCTGCTACCTTAGATGCTGAGAAGTTTTCAGGTTATTTCTTTGACTGTAACATCTTCACTATACCGGGGAGAACTTTTCCTGTAGAGATACTATACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTCTAATCACTGTTCTACAGATTCACTTAACAGAACCAGAAGGGGACATCCTTCTCTTCTTGACGGGACAGGAAGAAATTGATTTCTCATGCCAGTCACTTTTTGAGAGGATGAAAGGTCTTGGTAAAAATGTACCTGAGTTGATTATCTTACCAGTTTACAGTGCCCTTCCTAGTGAAATGCAGTCGAGGATTTTTGATCCAGCTCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACTAATATTGCTGAGGCGTCACTAACCATTGATGGGATATTTTATGTAATTGATCCTGGATTTGCAAAGCAAAATGTTTATAACCCTAAGCAAGGGCTGGATTCACTGGTCATAACCCCAATTTCACAAGCATCAGCCAAGCAAAGAGCTGGGCGCGCTGGGCGTACTGGGCCTGGAAAGTGTTACCGCCTCTACACTGAGAGTGCATACCGCAACGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGTTAATTACACTTACCATGAAAGCAATGGGGATAAATGATCTGTTGTCTTTTGATTTTATGGATCCTCCCTCACCCCAAGCACTCATTTCTGCTATGGAACAACTGTACAGTTTAGGAGCACTCGATGAGGAGGGGCTTCTCACCAAACTGGGTCGGAAAATGGCTGAGTTTCCTCTTGATCCACCACTATCTAAGATGCTACTGGCCAGTGTGGACCTTGGATGCAGTGATGAGATCTTGACCATGATTGCCATGATTCAGACGGGCAATATATTTTACAGGCCTAGGGAAAAACAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGACCATCTGACTTTACTTGCAGTGTATGAGGCTTGGAAAGCTAAGAACTTTTCAGGGCCATGGTGTTTTGAGAACTTCGTTCAGTCTCGATCCCTTAGGAGGGCACAGGATGTCAGAAAACAGCTTCTAAGTATAATGGATAAGTATAAACTGGATGTTGTGAGTGCTGGAAAGAATTTTACACAGATCAGGAAGGCAATTACGGCGGGATTTTTCTTCCATGGTGCTAGAAAGGACCCTCAGGAGGGGTATAGAACCTTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCGCTCTTCCAGAGACAACCAGATTGGATCATCTACCATGAGTTGGTTATGACTACGAAGGAGTATATGCGTGAGGTAACCGTCGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCAAAGTGGCAGACCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAGCGTATTGAACCGCTATATGACAGATACCATGAACCAAACTCTTGGCGTCTTAGTAAACGACGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1188

Amino Acids

135.57

Weight (kDa)

6.31

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 233 - 303 1.3e-11 S1 RNA binding domain
DEAD PF00270 542 - 691 1.2e-07 DEAD/DEAH box helicase
AAA_22 PF13401 550 - 675 2.9e-06 AAA domain
Helicase_C PF00271 735 - 865 8.7e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 927 - 955 8.2e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 956 - 1015 3e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1073 - 1149 8.3e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 714, 2505
Acc36I ACCTGC 2 cut(s) 1263, 1715
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1460, 3370
AccII CGCG 3 cut(s) 417, 1778, 2580
AciI CCGC 9 cut(s) 205, 667, 1078, 1776, 2029, 2611, 2635, 3236, 3509
AcoI YGGCCR 3 cut(s) 1079, 1773, 2892
AcsI RAATTY 4 cut(s) 152, 1808, 2667, 3205
AcuI CTGAAG 3 cut(s) 1568, 1824, 2640
AcyI GRCGYC 2 cut(s) 2441, 3544
AfaI GTAC 7 cut(s) 38, 1756, 1889, 1946, 2322, 2590, 2795
AjiI CACGTC 1 cut(s) 3560
AjnI CCWGG 6 cut(s) 1698, 2398, 2479, 2596, 2988, 3443
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
Alw21I GWGCWC 3 cut(s) 962, 1100, 2809
Alw26I GTCTC 9 cut(s) 447, 471, 624, 1451, 1670, 2026, 3024, 3120, 3334
AlwNI CAGNNNCTG 2 cut(s) 884, 1460
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 9 cut(s) 1010, 1079, 1506, 1773, 1867, 2594, 2892, 2969, 3086
ApeKI GCWGC 6 cut(s) 5, 1337, 1340, 1387, 1390, 2161
ApoI RAATTY 4 cut(s) 152, 1808, 2667, 3205
ArsI GACNNNNNNTTYG 4 cut(s) 236, 268, 973, 1005
Asp700I GAANNNNTTC 1 cut(s) 1632
AspA2I CCTAGG 1 cut(s) 2972
AspLEI GCGC 6 cut(s) 417, 1371, 2580, 2582, 3331, 3486
AspS9I GGNCC 6 cut(s) 1010, 1868, 2594, 2902, 3086, 3265
AsuC2I CCSGG 1 cut(s) 2124
AsuHPI GGTGA 8 cut(s) 419, 883, 1092, 1284, 1475, 1685, 2751, 2821
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 2 cut(s) 2902, 3265
AvrII CCTAGG 1 cut(s) 2972
AxyI CCTNAGG 3 cut(s) 1135, 3125, 3270
BaeGI GKGCMC 2 cut(s) 2356, 3136
BalI TGGCCA 1 cut(s) 2894
BamHI GGATCC 1 cut(s) 2749
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 962, 1100
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 302, 1553
Bbv12I GWGCWC 3 cut(s) 962, 1100, 2809
BbvCI CCTCAGC 2 cut(s) 1212, 2436
BbvI GCAGC 5 cut(s) 1327, 1349, 1399, 1402, 2173
BceAI ACGGC 1 cut(s) 3249
BciT130I CCWGG 6 cut(s) 1700, 2400, 2481, 2598, 2990, 3445
BclI TGATCA 1 cut(s) 2995
BcnI CCSGG 1 cut(s) 2124
BcoDI GTCTC 9 cut(s) 447, 471, 624, 1451, 1670, 2026, 3024, 3120, 3334
BfaI CTAG 5 cut(s) 83, 2361, 2973, 3258, 3437
BfmI CTRYAG 4 cut(s) 1281, 1785, 2139, 2204
BfuAI ACCTGC 2 cut(s) 1263, 1715
BglII AGATCT 1 cut(s) 2922
BisI GCNGC 8 cut(s) 6, 1079, 1338, 1341, 1388, 1391, 1776, 2162
BlnI CCTAGG 1 cut(s) 2972
BlsI GCNGC 8 cut(s) 7, 1080, 1339, 1342, 1389, 1392, 1777, 2163
BmcAI AGTACT 1 cut(s) 1946
Bme1390I CCNGG 7 cut(s) 1700, 2124, 2400, 2481, 2598, 2990, 3445
Bme18I GGWCC 2 cut(s) 2902, 3265
BmeT110I CYCGRG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 3560
BmgT120I GGNCC 6 cut(s) 1010, 1868, 2594, 2902, 3086, 3265
BmiI GGNNCC 3 cut(s) 181, 2751, 3267
BmrFI CCNGG 7 cut(s) 1700, 2124, 2400, 2481, 2598, 2990, 3445
BmrI ACTGGG 2 cut(s) 2601, 2846
BmuI ACTGGG 2 cut(s) 2601, 2846
BpiI GAAGAC 2 cut(s) 302, 1553
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpmI CTGGAG 1 cut(s) 335
Bpu10I CCTNAGC 3 cut(s) 1212, 2436, 2511
BpuEI CTTGAG 1 cut(s) 1604
BpuMI CCSGG 1 cut(s) 2124
BsaAI YACGTR 1 cut(s) 1766
BsaBI GATNNNNATC 3 cut(s) 546, 918, 1428
BsaHI GRCGYC 2 cut(s) 2441, 3544
BsaI GGTCTC 2 cut(s) 2026, 3024
BsaWI WCCGGW 2 cut(s) 929, 1102
BsaXI ACNNNNNCTCC 2 cut(s) 640, 670
Bse21I CCTNAGG 3 cut(s) 1135, 3125, 3270
Bse3DI GCAATG 1 cut(s) 2718
Bse8I GATNNNNATC 3 cut(s) 546, 918, 1428
BseBI CCWGG 6 cut(s) 1700, 2400, 2481, 2598, 2990, 3445
BseJI GATNNNNATC 3 cut(s) 546, 918, 1428
BseMI GCAATG 1 cut(s) 2718
BsePI GCGCGC 1 cut(s) 2578
BseRI GAGGAG 3 cut(s) 203, 666, 2831
BseSI GKGCMC 2 cut(s) 2356, 3136
BseXI GCAGC 5 cut(s) 1327, 1349, 1399, 1402, 2173
BseYI CCCAGC 2 cut(s) 2573, 2582
BsgI GTGCAG 3 cut(s) 1326, 1338, 1356
Bsh1236I CGCG 3 cut(s) 417, 1778, 2580
BshFI GGCC 9 cut(s) 1012, 1081, 1508, 1775, 1869, 2596, 2894, 2971, 3088
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 3 cut(s) 962, 1100, 2809
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 3 cut(s) 930, 1103, 2123
BslFI GGGAC 4 cut(s) 141, 2246, 2268, 2632
BsmAI GTCTC 9 cut(s) 447, 471, 624, 1451, 1670, 2026, 3024, 3120, 3334
BsmBI CGTCTC 1 cut(s) 624
BsmFI GGGAC 4 cut(s) 141, 2246, 2268, 2632
BsnI GGCC 9 cut(s) 1012, 1081, 1508, 1775, 1869, 2596, 2894, 2971, 3088
Bso31I GGTCTC 2 cut(s) 2026, 3024
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 5 cut(s) 962, 1100, 2356, 2809, 3136
Bsp1407I TGTACA 2 cut(s) 1754, 2793
Bsp19I CCATGG 2 cut(s) 3089, 3250
BspACI CCGC 9 cut(s) 205, 667, 1078, 1776, 2029, 2611, 2635, 3236, 3509
BspANI GGCC 9 cut(s) 1012, 1081, 1508, 1775, 1869, 2596, 2894, 2971, 3088
BspFNI CGCG 3 cut(s) 417, 1778, 2580
BspHI TCATGA 2 cut(s) 550, 1972
BspLI GGNNCC 3 cut(s) 181, 2751, 3267
BspMI ACCTGC 2 cut(s) 1263, 1715
BspQI GCTCTTC 3 cut(s) 950, 1212, 3338
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 2 cut(s) 2026, 3024
BsrDI GCAATG 1 cut(s) 2718
BsrGI TGTACA 2 cut(s) 1754, 2793
BssHII GCGCGC 1 cut(s) 2578
BssNI GRCGYC 2 cut(s) 2441, 3544
BssT1I CCWWGG 6 cut(s) 45, 1436, 2905, 2972, 3089, 3250
Bst2UI CCWGG 6 cut(s) 1700, 2400, 2481, 2598, 2990, 3445
BstACI GRCGYC 2 cut(s) 2441, 3544
BstAPI GCANNNNNTGC 1 cut(s) 1603
BstAUI TGTACA 2 cut(s) 1754, 2793
BstBAI YACGTR 1 cut(s) 1766
BstC8I GCNNGC 5 cut(s) 573, 1251, 1604, 2580, 3488
BstDSI CCRYGG 2 cut(s) 3089, 3250
BstEII GGTNACC 1 cut(s) 3403
BstENI CCTNNNNNAGG 1 cut(s) 2514
BstFNI CGCG 3 cut(s) 417, 1778, 2580
BstHHI GCGC 6 cut(s) 417, 1371, 2580, 2582, 3331, 3486
BstMAI GTCTC 9 cut(s) 447, 471, 624, 1451, 1670, 2026, 3024, 3120, 3334
BstMWI GCNNNNNNNGC 9 cut(s) 1009, 1387, 1603, 2579, 2584, 2593, 3068, 3233, 3496
BstNI CCWGG 6 cut(s) 1700, 2400, 2481, 2598, 2990, 3445
BstNSI RCATGY 2 cut(s) 575, 1519
BstPI GGTNACC 1 cut(s) 3403
BstSCI CCNGG 7 cut(s) 1698, 2122, 2398, 2479, 2596, 2988, 3443
BstSFI CTRYAG 4 cut(s) 1281, 1785, 2139, 2204
BstSLI GKGCMC 2 cut(s) 2356, 3136
BstUI CGCG 3 cut(s) 417, 1778, 2580
BstV1I GCAGC 5 cut(s) 1327, 1349, 1399, 1402, 2173
BstV2I GAAGAC 2 cut(s) 302, 1553
BstX2I RGATCY 4 cut(s) 1423, 1444, 2749, 2922
BstXI CCANNNNNNTGG 1 cut(s) 3540
BstYI RGATCY 4 cut(s) 1423, 1444, 2749, 2922
Bsu36I CCTNAGG 3 cut(s) 1135, 3125, 3270
BsuRI GGCC 9 cut(s) 1012, 1081, 1508, 1775, 1869, 2596, 2894, 2971, 3088
BtgI CCRYGG 2 cut(s) 3089, 3250
BtgZI GCGATG 1 cut(s) 1793
BtrI CACGTC 1 cut(s) 3560
BtsI GCAGTG 4 cut(s) 1647, 2920, 3057, 3331
BveI ACCTGC 2 cut(s) 1263, 1715
Cac8I GCNNGC 5 cut(s) 573, 1251, 1604, 2580, 3488
CaiI CAGNNNCTG 2 cut(s) 884, 1460
CciI TCATGA 2 cut(s) 550, 1972
CfoI GCGC 6 cut(s) 417, 1371, 2580, 2582, 3331, 3486
Cfr13I GGNCC 6 cut(s) 1010, 1868, 2594, 2902, 3086, 3265
CseI GACGC 3 cut(s) 2430, 3479, 3533
Csp6I GTAC 7 cut(s) 37, 1755, 1888, 1945, 2321, 2589, 2794
CviQI GTAC 7 cut(s) 37, 1755, 1888, 1945, 2321, 2589, 2794
EaeI YGGCCR 3 cut(s) 1079, 1773, 2892
Ecl136II GAGCTC 2 cut(s) 960, 1098
Eco130I CCWWGG 6 cut(s) 45, 1436, 2905, 2972, 3089, 3250
Eco147I AGGCCT 1 cut(s) 2971
Eco24I GRGCYC 2 cut(s) 962, 1100
Eco31I GGTCTC 2 cut(s) 2026, 3024
Eco47I GGWCC 2 cut(s) 2902, 3265
Eco53kI GAGCTC 2 cut(s) 960, 1098
Eco57I CTGAAG 3 cut(s) 1568, 1824, 2640
Eco81I CCTNAGG 3 cut(s) 1135, 3125, 3270
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 3403
EcoICRI GAGCTC 2 cut(s) 960, 1098
EcoNI CCTNNNNNAGG 1 cut(s) 2514
EcoO109I RGGNCCY 1 cut(s) 3265
EcoO65I GGTNACC 1 cut(s) 3403
EcoRII CCWGG 6 cut(s) 1698, 2398, 2479, 2596, 2988, 3443
EcoT14I CCWWGG 6 cut(s) 45, 1436, 2905, 2972, 3089, 3250
EcoT22I ATGCAT 1 cut(s) 2188
EcoT38I GRGCYC 2 cut(s) 962, 1100
ErhI CCWWGG 6 cut(s) 45, 1436, 2905, 2972, 3089, 3250
Esp3I CGTCTC 1 cut(s) 624
FaqI GGGAC 4 cut(s) 141, 2246, 2268, 2632
FauI CCCGC 1 cut(s) 3229
FbaI TGATCA 1 cut(s) 2995
Fnu4HI GCNGC 8 cut(s) 6, 1079, 1338, 1341, 1388, 1391, 1776, 2162
FriOI GRGCYC 2 cut(s) 962, 1100
Fsp4HI GCNGC 8 cut(s) 6, 1079, 1338, 1341, 1388, 1391, 1776, 2162
FspBI CTAG 5 cut(s) 83, 2361, 2973, 3258, 3437
GlaI GCGC 6 cut(s) 416, 1370, 2579, 2581, 3330, 3485
GluI GCNGC 8 cut(s) 6, 1079, 1338, 1341, 1388, 1391, 1776, 2162
GsaI CCCAGC 2 cut(s) 2577, 2586
GsuI CTGGAG 1 cut(s) 335
HaeIII GGCC 9 cut(s) 1012, 1081, 1508, 1775, 1869, 2596, 2894, 2971, 3088
HapII CCGG 3 cut(s) 930, 1103, 2123
HgaI GACGC 3 cut(s) 2430, 3479, 3533
HhaI GCGC 6 cut(s) 417, 1371, 2580, 2582, 3331, 3486
Hin1I GRCGYC 2 cut(s) 2441, 3544
Hin6I GCGC 6 cut(s) 415, 1369, 2578, 2580, 3329, 3484
HinP1I GCGC 6 cut(s) 415, 1369, 2578, 2580, 3329, 3484
HincII GTYRAC 1 cut(s) 3415
HindII GTYRAC 1 cut(s) 3415
HinfI GANTC 7 cut(s) 263, 1312, 1409, 1429, 2209, 2525, 2944
HpaII CCGG 3 cut(s) 930, 1103, 2123
HphI GGTGA 8 cut(s) 419, 883, 1092, 1284, 1475, 1685, 2751, 2821
Hpy166II GTNNAC 7 cut(s) 674, 1755, 1757, 2347, 2902, 3415, 3554
Hpy8I GTNNAC 7 cut(s) 674, 1755, 1757, 2347, 2902, 3415, 3554
Hpy99I CGWCG 2 cut(s) 3413, 3561
HpyCH4IV ACGT 4 cut(s) 144, 1765, 2044, 3559
HpyF10VI GCNNNNNNNGC 9 cut(s) 1009, 1387, 1603, 2579, 2584, 2593, 3068, 3233, 3496
HpySE526I ACGT 4 cut(s) 144, 1765, 2044, 3559
Hsp92I GRCGYC 2 cut(s) 2441, 3544
HspAI GCGC 6 cut(s) 415, 1369, 2578, 2580, 3329, 3484
Ksp22I TGATCA 1 cut(s) 2995
LguI GCTCTTC 3 cut(s) 950, 1212, 3338
LmnI GCTCC 4 cut(s) 1208, 1483, 2398, 2804
Lsp1109I GCAGC 5 cut(s) 1327, 1349, 1399, 1402, 2173
MaeI CTAG 5 cut(s) 83, 2361, 2973, 3258, 3437
MaeII ACGT 4 cut(s) 144, 1765, 2044, 3559
MflI RGATCY 4 cut(s) 1423, 1444, 2749, 2922
MhlI GDGCHC 5 cut(s) 962, 1100, 2356, 2809, 3136
MlsI TGGCCA 1 cut(s) 2894
MluNI TGGCCA 1 cut(s) 2894
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 2 cut(s) 1386, 2822
Mox20I TGGCCA 1 cut(s) 2894
Mph1103I ATGCAT 1 cut(s) 2188
MroXI GAANNNNTTC 1 cut(s) 1632
MscI TGGCCA 1 cut(s) 2894
MseI TTAA 9 cut(s) 306, 867, 1085, 1236, 1308, 1328, 1362, 2216, 2690
MslI CAYNNNNRTG 4 cut(s) 555, 687, 1736, 1992
Msp20I TGGCCA 1 cut(s) 2894
MspA1I CMGCKG 2 cut(s) 667, 1340
MspI CCGG 3 cut(s) 930, 1103, 2123
MspR9I CCNGG 7 cut(s) 1700, 2124, 2400, 2481, 2598, 2990, 3445
MvaI CCWGG 6 cut(s) 1700, 2400, 2481, 2598, 2990, 3445
MvnI CGCG 3 cut(s) 417, 1778, 2580
MwoI GCNNNNNNNGC 9 cut(s) 1009, 1387, 1603, 2579, 2584, 2593, 3068, 3233, 3496
NciI CCSGG 1 cut(s) 2124
NcoI CCATGG 2 cut(s) 3089, 3250
NlaIV GGNNCC 3 cut(s) 181, 2751, 3267
NmeAIII GCCGAG 3 cut(s) 208, 793, 1054
NmuCI GTSAC 6 cut(s) 764, 1015, 1290, 2053, 2283, 2442
NsiI ATGCAT 1 cut(s) 2188
NspI RCATGY 2 cut(s) 575, 1519
PaeI GCATGC 1 cut(s) 575
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 550, 1972
PauI GCGCGC 1 cut(s) 2578
PceI AGGCCT 1 cut(s) 2971
PciSI GCTCTTC 3 cut(s) 950, 1212, 3338
PdmI GAANNNNTTC 1 cut(s) 1632
PfeI GAWTC 6 cut(s) 1312, 1409, 1429, 2209, 2525, 2944
PflMI CCANNNNNTGG 2 cut(s) 1460, 3370
PfoI TCCNGGA 1 cut(s) 2479
PkrI GCNGC 8 cut(s) 7, 1080, 1339, 1342, 1389, 1392, 1777, 2163
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 1766
PpuMI RGGWCCY 1 cut(s) 3265
PsiI TTATAA 2 cut(s) 714, 2505
Psp124BI GAGCTC 2 cut(s) 962, 1100
Psp5II RGGWCCY 1 cut(s) 3265
Psp6I CCWGG 6 cut(s) 1698, 2398, 2479, 2596, 2988, 3443
PspEI GGTNACC 1 cut(s) 3403
PspFI CCCAGC 2 cut(s) 2573, 2582
PspGI CCWGG 6 cut(s) 1698, 2398, 2479, 2596, 2988, 3443
PspN4I GGNNCC 3 cut(s) 181, 2751, 3267
PspPI GGNCC 6 cut(s) 1010, 1868, 2594, 2902, 3086, 3265
PspPPI RGGWCCY 1 cut(s) 3265
PspXI VCTCGAGB 1 cut(s) 32
PstNI CAGNNNCTG 2 cut(s) 884, 1460
PsuI RGATCY 4 cut(s) 1423, 1444, 2749, 2922
PteI GCGCGC 1 cut(s) 2578
PvuII CAGCTG 1 cut(s) 1340
RsaI GTAC 7 cut(s) 38, 1756, 1889, 1946, 2322, 2590, 2795
RsaNI GTAC 7 cut(s) 37, 1755, 1888, 1945, 2321, 2589, 2794
RseI CAYNNNNRTG 4 cut(s) 555, 687, 1736, 1992
SacI GAGCTC 2 cut(s) 962, 1100
SapI GCTCTTC 3 cut(s) 950, 1212, 3338
SaqAI TTAA 9 cut(s) 306, 867, 1085, 1236, 1308, 1328, 1362, 2216, 2690
SatI GCNGC 8 cut(s) 6, 1079, 1338, 1341, 1388, 1391, 1776, 2162
Sau96I GGNCC 6 cut(s) 1010, 1868, 2594, 2902, 3086, 3265
ScaI AGTACT 1 cut(s) 1946
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 7 cut(s) 1700, 2124, 2400, 2481, 2598, 2990, 3445
SduI GDGCHC 5 cut(s) 962, 1100, 2356, 2809, 3136
SfcI CTRYAG 4 cut(s) 1281, 1785, 2139, 2204
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 2 cut(s) 2902, 3265
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 4 cut(s) 555, 687, 1736, 1992
SmlI CTYRAG 2 cut(s) 32, 1619
SmoI CTYRAG 2 cut(s) 32, 1619
SphI GCATGC 1 cut(s) 575
SseBI AGGCCT 1 cut(s) 2971
SsiI CCGC 9 cut(s) 205, 667, 1078, 1776, 2029, 2611, 2635, 3236, 3509
SspI AATATT 1 cut(s) 2431
SspMI CTAG 5 cut(s) 83, 2361, 2973, 3258, 3437
SstI GAGCTC 2 cut(s) 962, 1100
StuI AGGCCT 1 cut(s) 2971
StyD4I CCNGG 7 cut(s) 1698, 2122, 2398, 2479, 2596, 2988, 3443
StyI CCWWGG 6 cut(s) 45, 1436, 2905, 2972, 3089, 3250
TaiI ACGT 4 cut(s) 147, 1768, 2047, 3562
TaqI TCGA 6 cut(s) 33, 475, 918, 2375, 2811, 3118
TaqII GACCGA 2 cut(s) 408, 594
TatI WGTACW 4 cut(s) 1754, 1887, 1944, 2793
TauI GCSGC 2 cut(s) 1081, 1778
TfiI GAWTC 6 cut(s) 1312, 1409, 1429, 2209, 2525, 2944
Tru1I TTAA 9 cut(s) 306, 867, 1085, 1236, 1308, 1328, 1362, 2216, 2690
Tru9I TTAA 9 cut(s) 306, 867, 1085, 1236, 1308, 1328, 1362, 2216, 2690
TseFI GTSAC 6 cut(s) 764, 1015, 1290, 2053, 2283, 2442
TseI GCWGC 6 cut(s) 5, 1337, 1340, 1387, 1390, 2161
Tsp45I GTSAC 6 cut(s) 764, 1015, 1290, 2053, 2283, 2442
Van91I CCANNNNNTGG 2 cut(s) 1460, 3370
VpaK11BI GGWCC 2 cut(s) 2902, 3265
XagI CCTNNNNNAGG 1 cut(s) 2514
XapI RAATTY 4 cut(s) 152, 1808, 2667, 3205
XceI RCATGY 2 cut(s) 575, 1519
XcmI CCANNNNNNNNNTGG 3 cut(s) 86, 2710, 3427
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 2972
XmnI GAANNNNTTC 1 cut(s) 1632
XspI CTAG 5 cut(s) 83, 2361, 2973, 3258, 3437
ZrmI AGTACT 1 cut(s) 1946
Zsp2I ATGCAT 1 cut(s) 2188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.