FvH4_5g03230
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000706
Physical Location & Seq
Forward (+)
1915333 .. 1916372
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03230.t1

Sequence Viewer

Length: 945 bp
ATGGCTACATTCTCGGGTTGCAAAGAATTCCATATGGACGATATGGCTTTGGCATTCATCTTGGCTGACAAAGGGTTTGATGTATGGCTTGCGAACACTCGAGGAACAGACTCTAGTCGTGGACACAAATCACTTAGTGCTAGTGATCCTGCTTACTGGGAGTGGACATGGGAACAGTTGGCAACTTATGATCTTCCCGCTTTGTTCGATTATGTGAACAACCATACAGGACAACAATCACATTATGCAGGTCATTCCTTGGGAACGTTAACAGTACTAGCCGCCTTGTCCCAACAAAAATTGAGAAACTCAATAAGGTCAGCTGCTTTGCTTAGCCCAATTGCTCATCTTCGTCACATATCCACACCATTTGTAAGAGTTTCTACTCAAACGTATATGGCTGAGCAATTCTACATCTATGGTCTTCGAGAATTTCCTCCGCTAGGGCAATTAGAACAAACTCTGCAAACCATTCTTTGCATACATCCGGGAGTGGAGTGCTCCAACATTATGGCTGCTATAACAGGTCCAAATTGTTGTTTAAATCCTTCAAGTTTAGCTCTTTACTATAGACATACACCCCAATCGACTTCGACAAAGAATGTGGTGCATTTGTCTCAGTTGATCAGAAGTGGAACCGTAAGGATGTTATTTGACTACACTCTTCCATTTACAAATATGCAGCACTACGGACAGTTTACTCCTCCAGTGTACAACATGACAGACATACCAAAGGACATTCCTCTTTTCCTCGGTTTTGGAGGGAGAGATGCACTCTCAGACGTTCACGATGTGAAAGTTTTGCTTCGTGACCTCAGAAATCATGATAAAGACAAGCTTGTGGTAGAGTACATACAGGAGTATGCTCATATGGACTTTATTTTGGGTTGGAATGCCAATCAGAAAGTTTATGATCCTATGCTCAATTTCTTCGGGCAACATTGA

Protein Analysis

315

Amino Acids

35.6

Weight (kDa)

6.29

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 21 - 123 1.3e-10 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000706)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03230 FvH4_5g03471 FvH4_5g03472 FvH4_6g41950 FvH4_6g41951
malus_domestica MD09G1115700.v1.1 MD09G1115900.v1.1 MD17G1107600.v1.1 MD17G1107700.v1.1
prunus_persica Prupe.3G208700_v2.0.a1 Prupe.3G208800_v2.0.a1 Prupe.5G037500_v2.0.a1
pyrus_communis pycom09g04010 pycom09g04030 pycom17g10020 pycom17g10030
rosa_chinensis RchiOBHm_Chr2g0101861 RchiOBHm_Chr2g0157451 RchiOBHm_Chr2g0157461 RchiOBHm_Chr2g0157841 RchiOBHm_Chr2g0157851 RchiOBHm_Chr3g0484721 RchiOBHm_Chr3g0484731 RchiOBHm_Chr3g0484741 RchiOBHm_Chr3g0484751 RchiOBHm_Chr6g0253931 RchiOBHm_Chr6g0253991
rosa_laevigata RLG00000014950 RLG00000014954 RLG00000020989 RLG00000021024 RLG00000021026 RLG00000021027 RLG00000021029 RLG00000021030
rosa_multiflora Rmu_sc0000195.1_g000023 Rmu_sc0000195.1_g000028 Rmu_sc0001689.1_g000007 Rmu_sc0001716.1_g000018 Rmu_sc0002441.1_g000012 Rmu_sc0004191.1_g000065 Rmu_sc0004473.1_g000004
rosa_roxburghii Rroxscaffold_1G00036760 Rroxscaffold_1G00036780 Rroxscaffold_2G00091900 Rroxscaffold_2G00092180 Rroxscaffold_2G00092200 Rroxscaffold_2G00127230 Rroxscaffold_7G00211340 Rroxscaffold_7G00211380
rosa_rugosa Rorug02G0102500 Rorug02G0206400 Rorug02G0206500 Rorug02G0461400 Rorug02G0464100 Rorug05G0548600 Rorug05G0548800
rosa_samantha Rh2BG155500 Rh2BG540100 Rh2BG540200 Rh2BG543500 Rh2BG543700 Rh2BG543800 Rh3CG296800 Rh6AG064700 Rh6AG065300
rosa_wichuraiana Rw2G020570 Rw2G043520 Rw2G043530 Rw2G043770 Rw2G043780 Rw3G023530 Rw3G023540 Rw6G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 239
AciI CCGC 3 cut(s) 198, 282, 440
AclI AACGTT 1 cut(s) 266
AclWI GGATC 2 cut(s) 140, 908
AcsI RAATTY 2 cut(s) 26, 431
AdeI CACNNNGTG 2 cut(s) 137, 793
AfaI GTAC 3 cut(s) 276, 713, 851
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 1 cut(s) 552
AluBI AGCT 3 cut(s) 323, 560, 838
AluI AGCT 3 cut(s) 323, 560, 838
Alw21I GWGCWC 1 cut(s) 503
Alw26I GTCTC 1 cut(s) 621
AlwI GGATC 2 cut(s) 140, 908
Ama87I CYCGRG 2 cut(s) 13, 99
ApeKI GCWGC 3 cut(s) 323, 515, 682
ApoI RAATTY 2 cut(s) 26, 431
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspS9I GGNCC 1 cut(s) 527
AsuC2I CCSGG 1 cut(s) 489
AvaI CYCGRG 2 cut(s) 13, 99
AvaII GGWCC 1 cut(s) 527
BbsI GAAGAC 1 cut(s) 416
Bbv12I GWGCWC 1 cut(s) 503
BbvI GCAGC 3 cut(s) 310, 502, 694
BclI TGATCA 1 cut(s) 624
BcnI CCSGG 1 cut(s) 489
BcoDI GTCTC 1 cut(s) 621
BfaI CTAG 4 cut(s) 114, 141, 278, 443
BfmI CTRYAG 1 cut(s) 568
BfuAI ACCTGC 1 cut(s) 239
BisI GCNGC 4 cut(s) 282, 324, 516, 683
BlpI GCTNAGC 2 cut(s) 332, 402
BlsI GCNGC 4 cut(s) 283, 325, 517, 684
BmcAI AGTACT 1 cut(s) 276
Bme1390I CCNGG 1 cut(s) 489
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 2 cut(s) 13, 99
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 1 cut(s) 637
BmrFI CCNGG 1 cut(s) 489
BmrI ACTGGG 1 cut(s) 166
BmsI GCATC 1 cut(s) 760
BmuI ACTGGG 1 cut(s) 166
BoxI GACNNNNGTC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 416
BplI GAGNNNNNCTC 2 cut(s) 759, 791
BpmI CTGGAG 1 cut(s) 690
Bpu1102I GCTNAGC 2 cut(s) 332, 402
BpuMI CCSGG 1 cut(s) 489
BsaJI CCNNGG 2 cut(s) 258, 751
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse1I ACTGG 2 cut(s) 161, 707
BseDI CCNNGG 2 cut(s) 258, 751
BseGI GGATG 2 cut(s) 484, 651
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMII CTCAG 4 cut(s) 393, 632, 792, 829
BseNI ACTGG 2 cut(s) 161, 707
BseRI GAGGAG 1 cut(s) 693
BseXI GCAGC 3 cut(s) 310, 502, 694
BsiHKAI GWGCWC 1 cut(s) 503
BsiHKCI CYCGRG 2 cut(s) 13, 99
BsiSI CCGG 1 cut(s) 488
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 1 cut(s) 621
BsmFI GGGAC 1 cut(s) 274
BsmI GAATGC 2 cut(s) 53, 898
BsoBI CYCGRG 2 cut(s) 13, 99
Bsp1286I GDGCHC 1 cut(s) 503
Bsp1407I TGTACA 1 cut(s) 711
Bsp143I GATC 4 cut(s) 145, 190, 624, 913
Bsp1720I GCTNAGC 2 cut(s) 332, 402
BspACI CCGC 3 cut(s) 198, 282, 440
BspCNI CTCAG 4 cut(s) 394, 631, 791, 828
BspHI TCATGA 1 cut(s) 823
BspLI GGNNCC 1 cut(s) 637
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 2 cut(s) 140, 908
BsrGI TGTACA 1 cut(s) 711
BsrI ACTGG 2 cut(s) 161, 707
BssECI CCNNGG 2 cut(s) 258, 751
BssMI GATC 4 cut(s) 145, 190, 624, 913
BssT1I CCWWGG 1 cut(s) 258
Bst4CI ACNGT 4 cut(s) 177, 274, 640, 696
Bst6I CTCTTC 1 cut(s) 669
BstAUI TGTACA 1 cut(s) 711
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 6 cut(s) 134, 332, 402, 618, 778, 815
BstENI CCTNNNNNAGG 1 cut(s) 441
BstF5I GGATG 2 cut(s) 484, 651
BstKTI GATC 4 cut(s) 148, 193, 627, 916
BstMAI GTCTC 1 cut(s) 621
BstMBI GATC 4 cut(s) 145, 190, 624, 913
BstPAI GACNNNNGTC 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 568
BstV1I GCAGC 3 cut(s) 310, 502, 694
BstV2I GAAGAC 1 cut(s) 416
BstXI CCANNNNNNTGG 1 cut(s) 511
BtsCI GGATG 2 cut(s) 484, 651
BtsIMutI CAGTG 1 cut(s) 714
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 1 cut(s) 90
CciI TCATGA 1 cut(s) 823
Cfr13I GGNCC 1 cut(s) 527
Csp6I GTAC 3 cut(s) 275, 712, 850
CspCI CAANNNNNGTGG 4 cut(s) 352, 387, 585, 620
CviAII CATG 3 cut(s) 168, 718, 824
CviQI GTAC 3 cut(s) 275, 712, 850
DdeI CTNAG 6 cut(s) 134, 332, 402, 618, 778, 815
DpnI GATC 4 cut(s) 147, 192, 626, 915
DpnII GATC 4 cut(s) 145, 190, 624, 913
DraI TTTAAA 1 cut(s) 543
DraIII CACNNNGTG 2 cut(s) 137, 793
Eam1104I CTCTTC 1 cut(s) 669
EarI CTCTTC 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 527
Eco88I CYCGRG 2 cut(s) 13, 99
EcoNI CCTNNNNNAGG 1 cut(s) 441
EcoRI GAATTC 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 3 cut(s) 171, 721, 827
FalI AAGNNNNNCTT 4 cut(s) 789, 821, 822, 854
FaqI GGGAC 1 cut(s) 274
FatI CATG 3 cut(s) 167, 717, 823
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 2 cut(s) 33, 870
FbaI TGATCA 1 cut(s) 624
Fnu4HI GCNGC 4 cut(s) 282, 324, 516, 683
FokI GGATG 2 cut(s) 471, 658
Fsp4HI GCNGC 4 cut(s) 282, 324, 516, 683
FspBI CTAG 4 cut(s) 114, 141, 278, 443
GluI GCNGC 4 cut(s) 282, 324, 516, 683
GsuI CTGGAG 1 cut(s) 690
HapII CCGG 1 cut(s) 488
Hin1II CATG 3 cut(s) 171, 721, 827
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 1 cut(s) 110
HpaI GTTAAC 1 cut(s) 270
HpaII CCGG 1 cut(s) 488
Hpy166II GTNNAC 7 cut(s) 122, 165, 217, 270, 699, 712, 787
Hpy188I TCNGA 4 cut(s) 629, 781, 818, 903
Hpy188III TCNNGA 4 cut(s) 428, 788, 809, 824
Hpy8I GTNNAC 7 cut(s) 122, 165, 217, 270, 699, 712, 787
HpyAV CCTTC 1 cut(s) 558
HpyCH4III ACNGT 4 cut(s) 177, 274, 640, 696
HpyCH4IV ACGT 3 cut(s) 266, 392, 783
HpyCH4V TGCA 7 cut(s) 21, 248, 466, 480, 610, 682, 773
HpyF3I CTNAG 6 cut(s) 134, 332, 402, 618, 778, 815
HpySE526I ACGT 3 cut(s) 266, 392, 783
Hsp92II CATG 3 cut(s) 171, 721, 827
Ksp22I TGATCA 1 cut(s) 624
KspAI GTTAAC 1 cut(s) 270
Kzo9I GATC 4 cut(s) 145, 190, 624, 913
LmnI GCTCC 1 cut(s) 506
LpnPI CCDG 8 cut(s) 142, 162, 213, 234, 501, 510, 720, 842
Lsp1109I GCAGC 3 cut(s) 310, 502, 694
LweI GCATC 1 cut(s) 760
MaeI CTAG 4 cut(s) 114, 141, 278, 443
MaeII ACGT 3 cut(s) 266, 392, 783
MaeIII GTNAC 2 cut(s) 353, 809
MalI GATC 4 cut(s) 147, 192, 626, 915
MboI GATC 4 cut(s) 145, 190, 624, 913
MboII GAAGA 5 cut(s) 185, 341, 416, 656, 922
MfeI CAATTG 1 cut(s) 339
MhlI GDGCHC 1 cut(s) 503
MluCI AATT 8 cut(s) 26, 299, 339, 407, 431, 449, 532, 925
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 2 cut(s) 528, 869
MnlI CCTC 7 cut(s) 95, 447, 714, 753, 755, 761, 824
MseI TTAA 2 cut(s) 269, 542
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 1 cut(s) 488
MspR9I CCNGG 1 cut(s) 489
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 2 cut(s) 53, 898
NciI CCSGG 1 cut(s) 489
NdeI CATATG 2 cut(s) 33, 870
NdeII GATC 4 cut(s) 145, 190, 624, 913
NlaIII CATG 3 cut(s) 171, 721, 827
NlaIV GGNNCC 1 cut(s) 637
NmuCI GTSAC 2 cut(s) 353, 809
PaeR7I CTCGAG 1 cut(s) 99
PagI TCATGA 1 cut(s) 823
PctI GAATGC 2 cut(s) 53, 898
PfoI TCCNGGA 1 cut(s) 487
PkrI GCNGC 4 cut(s) 283, 325, 517, 684
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PshAI GACNNNNGTC 1 cut(s) 114
Psp1406I AACGTT 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 637
PspPI GGNCC 1 cut(s) 527
PspXI VCTCGAGB 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 323
RsaI GTAC 3 cut(s) 276, 713, 851
RsaNI GTAC 3 cut(s) 275, 712, 850
SaqAI TTAA 2 cut(s) 269, 542
SatI GCNGC 4 cut(s) 282, 324, 516, 683
Sau3AI GATC 4 cut(s) 145, 190, 624, 913
Sau96I GGNCC 1 cut(s) 527
ScaI AGTACT 1 cut(s) 276
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 1 cut(s) 503
SfaNI GCATC 1 cut(s) 760
SfcI CTRYAG 1 cut(s) 568
Sfr274I CTCGAG 1 cut(s) 99
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
Sse9I AATT 8 cut(s) 26, 299, 339, 407, 431, 449, 532, 925
SsiI CCGC 3 cut(s) 198, 282, 440
SspMI CTAG 4 cut(s) 114, 141, 278, 443
StyD4I CCNGG 1 cut(s) 487
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 4 cut(s) 177, 274, 640, 696
TaiI ACGT 3 cut(s) 269, 395, 786
TaqI TCGA 5 cut(s) 100, 207, 427, 587, 593
TasI AATT 8 cut(s) 26, 299, 339, 407, 431, 449, 532, 925
TatI WGTACW 3 cut(s) 274, 711, 849
TauI GCSGC 1 cut(s) 284
Tru1I TTAA 2 cut(s) 269, 542
Tru9I TTAA 2 cut(s) 269, 542
TscAI CASTG 1 cut(s) 714
TseFI GTSAC 2 cut(s) 353, 809
TseI GCWGC 3 cut(s) 323, 515, 682
Tsp45I GTSAC 2 cut(s) 353, 809
TspDTI ATGAA 1 cut(s) 46
TspGWI ACGGA 1 cut(s) 705
TspRI CASTG 1 cut(s) 714
VpaK11BI GGWCC 1 cut(s) 527
XagI CCTNNNNNAGG 1 cut(s) 441
XapI RAATTY 2 cut(s) 26, 431
XhoI CTCGAG 1 cut(s) 99
XspI CTAG 4 cut(s) 114, 141, 278, 443
ZrmI AGTACT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.