Rroxscaffold_2G00092180
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000706
Physical Location & Seq
Reverse (-)
13728683 .. 13730292
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00092180.1

Sequence Viewer

Length: 1197 bp
ATGGCCAAGTACCCTTCAGCTAGTATTCTACTTGTCGTGGCTCTAGTTTGTGTTTCAGGAGTTGTACAAACCAGAGGTCATGATATTCACAATGGTATTTGCAAATCACTTGTGGAGACACGAGGATATATTTGCCAAGAACATCAAGTAACAACAGAAGATGGTTACATTCTCGGGTTGCAGAGAATTCCATATGGACGGTCTGGTAGAAATTCAGCCACAGATCAGAAGCCGCCTGTTTTGTTACAACATGGGCTTATAATGGATGCTGCAGCATGGCTACTCAATCCACCGGATCAGGCTTTGGCGTTCATCTTGGCTGACAAAGGGTTTGATGTATGGCTTGCTAATTCTCGAGGAACGGACTCTAGCCGTGGACACAAATCACTTACTACTAATGATCCTGCTTATTGGGAATGGACATGGGAACAGTTGGCTGCTTATGATCTTCCTGCGTTGTTCAATTATGTGAACAAGCAAACAGGACAGAAGTCACACTACGTTGGCCATTCCTTAGGAACATTGACAATACTAATTGCCTTGTCCCAACAAAAATTAAGAAACTCATTGAGATCAGCTGCTTTGCTTACCCCAGTTGCTTATCTGGGTCAGTTATCCTCACTGTTTATAAGAGTTGCTACTCAAACATATCATGCTGAGCTATTCTACATCGTTGGTGTCCGGGAATTTCCTCCGCTGCCGCGATTAGAACGAACTCTGCAGACCACTATTTGCGCACAACCGGGCGTGGACTGTTCCAACTTTCTGGCTGCTATAACAGGTCCAAATTGTTGCCTAAATCCTTCAAGTTTAGATCTTTACTATAATCACACACCACAATCCACTGCCACAAAGAATTTCATACATGTGTCTCAGATGATCAGAAGTGGAACCATAAGAATGTTCGATTACACTCTTCCACTCACAAATCTGCAGCACTACAATACGTTTACTCCTCCAGCGTACAACATGGCAAACATTCAGAAAGACATTCCTCTTTTCCTCAGCTATGGAGGGAGAGATGCACTTTCAGACGTCAACGATGTCAAGACTTTGCTTAATGACCTCAATGATCATGACAAGGACAAGCTTGTCGAACAATACATAGAGGAGTATGCTCATTATGATTACATTATGGGTGAGAATGTCCGTCAGAAAGTGTATGATCCTCTGCTCGCTTTCTTCAGGCAACATTGA

Protein Analysis

398

Amino Acids

44.72

Weight (kDa)

6.52

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 36 - 96 8.1e-21 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 78 - 210 3.1e-11 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000706)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03230 FvH4_5g03471 FvH4_5g03472 FvH4_6g41950 FvH4_6g41951
malus_domestica MD09G1115700.v1.1 MD09G1115900.v1.1 MD17G1107600.v1.1 MD17G1107700.v1.1
prunus_persica Prupe.3G208700_v2.0.a1 Prupe.3G208800_v2.0.a1 Prupe.5G037500_v2.0.a1
pyrus_communis pycom09g04010 pycom09g04030 pycom17g10020 pycom17g10030
rosa_chinensis RchiOBHm_Chr2g0101861 RchiOBHm_Chr2g0157451 RchiOBHm_Chr2g0157461 RchiOBHm_Chr2g0157841 RchiOBHm_Chr2g0157851 RchiOBHm_Chr3g0484721 RchiOBHm_Chr3g0484731 RchiOBHm_Chr3g0484741 RchiOBHm_Chr3g0484751 RchiOBHm_Chr6g0253931 RchiOBHm_Chr6g0253991
rosa_laevigata RLG00000014950 RLG00000014954 RLG00000020989 RLG00000021024 RLG00000021026 RLG00000021027 RLG00000021029 RLG00000021030
rosa_multiflora Rmu_sc0000195.1_g000023 Rmu_sc0000195.1_g000028 Rmu_sc0001689.1_g000007 Rmu_sc0001716.1_g000018 Rmu_sc0002441.1_g000012 Rmu_sc0004191.1_g000065 Rmu_sc0004473.1_g000004
rosa_roxburghii Rroxscaffold_1G00036760 Rroxscaffold_1G00036780 Rroxscaffold_2G00091900 Rroxscaffold_2G00092180 Rroxscaffold_2G00092200 Rroxscaffold_2G00127230 Rroxscaffold_7G00211340 Rroxscaffold_7G00211380
rosa_rugosa Rorug02G0102500 Rorug02G0206400 Rorug02G0206500 Rorug02G0461400 Rorug02G0464100 Rorug05G0548600 Rorug05G0548800
rosa_samantha Rh2BG155500 Rh2BG540100 Rh2BG540200 Rh2BG543500 Rh2BG543700 Rh2BG543800 Rh3CG296800 Rh6AG064700 Rh6AG065300
rosa_wichuraiana Rw2G020570 Rw2G043520 Rw2G043530 Rw2G043770 Rw2G043780 Rw3G023530 Rw3G023540 Rw6G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 260, 629
AasI GACNNNNNNGTC 1 cut(s) 1091
AatII GACGTC 1 cut(s) 1038
Acc16I TGCGCA 1 cut(s) 736
AccII CGCG 1 cut(s) 703
AciI CCGC 3 cut(s) 233, 695, 701
AclWI GGATC 3 cut(s) 303, 395, 1160
AcoI YGGCCR 2 cut(s) 3, 505
AcsI RAATTY 4 cut(s) 186, 211, 686, 856
AcuI CTGAAG 1 cut(s) 1168
AcyI GRCGYC 1 cut(s) 1035
AfaI GTAC 3 cut(s) 11, 66, 965
AflIII ACRYGT 1 cut(s) 865
AgsI TTSAA 2 cut(s) 463, 807
AluBI AGCT 5 cut(s) 20, 578, 661, 1008, 1090
AluI AGCT 5 cut(s) 20, 578, 661, 1008, 1090
Alw26I GTCTC 2 cut(s) 110, 876
AlwI GGATC 3 cut(s) 303, 395, 1160
Ama87I CYCGRG 2 cut(s) 173, 354
AoxI GGCC 2 cut(s) 3, 505
ApeKI GCWGC 7 cut(s) 269, 272, 437, 578, 697, 770, 934
ApoI RAATTY 4 cut(s) 186, 211, 686, 856
ArsI GACNNNNNNTTYG 4 cut(s) 61, 93, 314, 346
AspLEI GCGC 1 cut(s) 737
AspS9I GGNCC 1 cut(s) 782
AsuC2I CCSGG 2 cut(s) 683, 744
AsuHPI GGTGA 1 cut(s) 1151
AvaI CYCGRG 2 cut(s) 173, 354
AvaII GGWCC 1 cut(s) 782
AxyI CCTNAGG 1 cut(s) 514
BalI TGGCCA 2 cut(s) 5, 507
BarI GAAGNNNNNNTAC 2 cut(s) 482, 514
BauI CACGAG 1 cut(s) 120
BbvCI CCTCAGC 1 cut(s) 1004
BbvI GCAGC 7 cut(s) 256, 284, 424, 565, 684, 757, 946
BccI CCATC 1 cut(s) 155
BceAI ACGGC 1 cut(s) 357
BclI TGATCA 2 cut(s) 879, 1072
BcnI CCSGG 2 cut(s) 683, 744
BcoDI GTCTC 2 cut(s) 110, 876
BfaI CTAG 3 cut(s) 21, 44, 369
BfmI CTRYAG 3 cut(s) 270, 719, 932
BglII AGATCT 1 cut(s) 814
BisI GCNGC 9 cut(s) 233, 270, 273, 438, 579, 698, 701, 771, 935
BlpI GCTNAGC 1 cut(s) 657
BlsI GCNGC 9 cut(s) 234, 271, 274, 439, 580, 699, 702, 772, 936
Bme1390I CCNGG 2 cut(s) 683, 744
Bme18I GGWCC 1 cut(s) 782
BmeT110I CYCGRG 2 cut(s) 173, 354
BmgT120I GGNCC 1 cut(s) 782
BmiI GGNNCC 1 cut(s) 892
BmrFI CCNGG 2 cut(s) 683, 744
BmrI ACTGGG 1 cut(s) 587
BmsI GCATC 2 cut(s) 256, 1012
BmuI ACTGGG 1 cut(s) 587
BoxI GACNNNNGTC 1 cut(s) 490
BpmI CTGGAG 1 cut(s) 942
Bpu10I CCTNAGC 1 cut(s) 1004
Bpu1102I GCTNAGC 1 cut(s) 657
BpuMI CCSGG 2 cut(s) 683, 744
BsaHI GRCGYC 1 cut(s) 1035
BsaJI CCNNGG 1 cut(s) 373
BsaWI WCCGGW 1 cut(s) 292
BsaXI ACNNNNNCTCC 2 cut(s) 937, 967
Bse1I ACTGG 1 cut(s) 593
Bse21I CCTNAGG 1 cut(s) 514
BseDI CCNNGG 1 cut(s) 373
BseGI GGATG 1 cut(s) 271
BseMII CTCAG 3 cut(s) 648, 887, 1018
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 2 cut(s) 945, 1124
BseXI GCAGC 7 cut(s) 256, 284, 424, 565, 684, 757, 946
Bsh1236I CGCG 1 cut(s) 703
BshFI GGCC 2 cut(s) 5, 507
BsiHKCI CYCGRG 2 cut(s) 173, 354
BsiSI CCGG 3 cut(s) 293, 682, 743
BslFI GGGAC 1 cut(s) 529
BsmAI GTCTC 2 cut(s) 110, 876
BsmFI GGGAC 1 cut(s) 529
BsnI GGCC 2 cut(s) 5, 507
BsoBI CYCGRG 2 cut(s) 173, 354
Bsp1407I TGTACA 1 cut(s) 64
Bsp143I GATC 9 cut(s) 223, 295, 400, 445, 572, 814, 879, 1072, 1165
Bsp1720I GCTNAGC 1 cut(s) 657
BspACI CCGC 3 cut(s) 233, 695, 701
BspANI GGCC 2 cut(s) 5, 507
BspCNI CTCAG 3 cut(s) 649, 886, 1017
BspFNI CGCG 1 cut(s) 703
BspHI TCATGA 2 cut(s) 79, 1075
BspLI GGNNCC 1 cut(s) 892
BspMAI CTGCAG 3 cut(s) 274, 723, 936
BspPI GGATC 3 cut(s) 303, 395, 1160
BsrGI TGTACA 1 cut(s) 64
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 1 cut(s) 373
BssMI GATC 9 cut(s) 223, 295, 400, 445, 572, 814, 879, 1072, 1165
BssNI GRCGYC 1 cut(s) 1035
BssSI CACGAG 1 cut(s) 120
Bst2BI CACGAG 1 cut(s) 120
Bst4CI ACNGT 4 cut(s) 201, 432, 624, 755
Bst6I CTCTTC 1 cut(s) 921
BstACI GRCGYC 1 cut(s) 1035
BstAUI TGTACA 1 cut(s) 64
BstC8I GCNNGC 2 cut(s) 345, 1176
BstDEI CTNAG 4 cut(s) 514, 657, 873, 1004
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 1 cut(s) 703
BstHHI GCGC 1 cut(s) 737
BstKTI GATC 9 cut(s) 226, 298, 403, 448, 575, 817, 882, 1075, 1168
BstMAI GTCTC 2 cut(s) 110, 876
BstMBI GATC 9 cut(s) 223, 295, 400, 445, 572, 814, 879, 1072, 1165
BstNSI RCATGY 1 cut(s) 869
BstPAI GACNNNNGTC 1 cut(s) 490
BstSCI CCNGG 2 cut(s) 681, 742
BstSFI CTRYAG 3 cut(s) 270, 719, 932
BstUI CGCG 1 cut(s) 703
BstV1I GCAGC 7 cut(s) 256, 284, 424, 565, 684, 757, 946
BstX2I RGATCY 1 cut(s) 814
BstXI CCANNNNNNTGG 1 cut(s) 766
BstYI RGATCY 1 cut(s) 814
Bsu36I CCTNAGG 1 cut(s) 514
BsuRI GGCC 2 cut(s) 5, 507
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 843
BtsIMutI CAGTG 2 cut(s) 620, 843
Cac8I GCNNGC 2 cut(s) 345, 1176
CciI TCATGA 2 cut(s) 79, 1075
CfoI GCGC 1 cut(s) 737
Cfr13I GGNCC 1 cut(s) 782
Csp6I GTAC 3 cut(s) 10, 65, 964
CviAII CATG 8 cut(s) 80, 251, 276, 423, 653, 866, 970, 1076
CviQI GTAC 3 cut(s) 10, 65, 964
DdeI CTNAG 4 cut(s) 514, 657, 873, 1004
DpnI GATC 9 cut(s) 225, 297, 402, 447, 574, 816, 881, 1074, 1167
DpnII GATC 9 cut(s) 223, 295, 400, 445, 572, 814, 879, 1072, 1165
DrdI GACNNNNNNGTC 1 cut(s) 1091
DseDI GACNNNNNNGTC 1 cut(s) 1091
EaeI YGGCCR 2 cut(s) 3, 505
Eam1104I CTCTTC 1 cut(s) 921
EarI CTCTTC 1 cut(s) 921
Eco47I GGWCC 1 cut(s) 782
Eco57I CTGAAG 1 cut(s) 1168
Eco81I CCTNAGG 1 cut(s) 514
Eco88I CYCGRG 2 cut(s) 173, 354
EcoRI GAATTC 1 cut(s) 186
FaeI CATG 8 cut(s) 83, 254, 279, 426, 656, 869, 973, 1079
FaqI GGGAC 1 cut(s) 529
FatI CATG 8 cut(s) 79, 250, 275, 422, 652, 865, 969, 1075
FauNDI CATATG 1 cut(s) 193
FbaI TGATCA 2 cut(s) 879, 1072
Fnu4HI GCNGC 9 cut(s) 233, 270, 273, 438, 579, 698, 701, 771, 935
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 9 cut(s) 233, 270, 273, 438, 579, 698, 701, 771, 935
FspBI CTAG 3 cut(s) 21, 44, 369
FspI TGCGCA 1 cut(s) 736
GlaI GCGC 1 cut(s) 736
GluI GCNGC 9 cut(s) 233, 270, 273, 438, 579, 698, 701, 771, 935
GsuI CTGGAG 1 cut(s) 942
HaeIII GGCC 2 cut(s) 5, 507
HapII CCGG 3 cut(s) 293, 682, 743
HhaI GCGC 1 cut(s) 737
Hin1I GRCGYC 1 cut(s) 1035
Hin1II CATG 8 cut(s) 83, 254, 279, 426, 656, 869, 973, 1079
Hin6I GCGC 1 cut(s) 735
HinP1I GCGC 1 cut(s) 735
HincII GTYRAC 1 cut(s) 1039
HindII GTYRAC 1 cut(s) 1039
HindIII AAGCTT 1 cut(s) 1088
HinfI GANTC 1 cut(s) 365
HpaII CCGG 3 cut(s) 293, 682, 743
HphI GGTGA 1 cut(s) 1151
Hpy166II GTNNAC 5 cut(s) 377, 472, 751, 951, 1039
Hpy188I TCNGA 6 cut(s) 228, 876, 884, 984, 1033, 1155
Hpy188III TCNNGA 5 cut(s) 57, 80, 354, 1048, 1076
Hpy8I GTNNAC 5 cut(s) 377, 472, 751, 951, 1039
HpyAV CCTTC 2 cut(s) 24, 813
HpyCH4III ACNGT 4 cut(s) 201, 432, 624, 755
HpyCH4IV ACGT 3 cut(s) 501, 947, 1035
HpyCH4V TGCA 6 cut(s) 102, 181, 272, 721, 934, 1025
HpyF3I CTNAG 4 cut(s) 514, 657, 873, 1004
HpySE526I ACGT 3 cut(s) 501, 947, 1035
Hsp92I GRCGYC 1 cut(s) 1035
Hsp92II CATG 8 cut(s) 83, 254, 279, 426, 656, 869, 973, 1079
HspAI GCGC 1 cut(s) 735
Ksp22I TGATCA 2 cut(s) 879, 1072
Kzo9I GATC 9 cut(s) 223, 295, 400, 445, 572, 814, 879, 1072, 1165
Lsp1109I GCAGC 7 cut(s) 256, 284, 424, 565, 684, 757, 946
LweI GCATC 2 cut(s) 256, 1012
MaeI CTAG 3 cut(s) 21, 44, 369
MaeII ACGT 3 cut(s) 501, 947, 1035
MaeIII GTNAC 4 cut(s) 148, 164, 243, 492
MalI GATC 9 cut(s) 225, 297, 402, 447, 574, 816, 881, 1074, 1167
MboI GATC 9 cut(s) 223, 295, 400, 445, 572, 814, 879, 1072, 1165
MboII GAAGA 4 cut(s) 170, 440, 908, 1174
MflI RGATCY 1 cut(s) 814
MlsI TGGCCA 2 cut(s) 5, 507
MluCI AATT 9 cut(s) 186, 211, 349, 463, 534, 554, 686, 787, 856
MluNI TGGCCA 2 cut(s) 5, 507
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 783
Mox20I TGGCCA 2 cut(s) 5, 507
MscI TGGCCA 2 cut(s) 5, 507
MseI TTAA 2 cut(s) 557, 1059
MslI CAYNNNNRTG 2 cut(s) 866, 899
Msp20I TGGCCA 2 cut(s) 5, 507
MspA1I CMGCKG 2 cut(s) 578, 697
MspI CCGG 3 cut(s) 293, 682, 743
MspR9I CCNGG 2 cut(s) 683, 744
MvnI CGCG 1 cut(s) 703
NciI CCSGG 2 cut(s) 683, 744
NdeI CATATG 1 cut(s) 193
NdeII GATC 9 cut(s) 223, 295, 400, 445, 572, 814, 879, 1072, 1165
NlaIII CATG 8 cut(s) 83, 254, 279, 426, 656, 869, 973, 1079
NlaIV GGNNCC 1 cut(s) 892
NmuCI GTSAC 1 cut(s) 492
NsbI TGCGCA 1 cut(s) 736
NspI RCATGY 1 cut(s) 869
PaeR7I CTCGAG 1 cut(s) 354
PagI TCATGA 2 cut(s) 79, 1075
PciI ACATGT 1 cut(s) 865
PfoI TCCNGGA 1 cut(s) 681
PkrI GCNGC 9 cut(s) 234, 271, 274, 439, 580, 699, 702, 772, 936
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
PscI ACATGT 1 cut(s) 865
PshAI GACNNNNGTC 1 cut(s) 490
PsiI TTATAA 2 cut(s) 260, 629
PspN4I GGNNCC 1 cut(s) 892
PspPI GGNCC 1 cut(s) 782
PstI CTGCAG 3 cut(s) 274, 723, 936
PsuI RGATCY 1 cut(s) 814
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 3 cut(s) 11, 66, 965
RsaNI GTAC 3 cut(s) 10, 65, 964
RseI CAYNNNNRTG 2 cut(s) 866, 899
SaqAI TTAA 2 cut(s) 557, 1059
SatI GCNGC 9 cut(s) 233, 270, 273, 438, 579, 698, 701, 771, 935
Sau3AI GATC 9 cut(s) 223, 295, 400, 445, 572, 814, 879, 1072, 1165
Sau96I GGNCC 1 cut(s) 782
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 2 cut(s) 683, 744
SfaNI GCATC 2 cut(s) 256, 1012
SfcI CTRYAG 3 cut(s) 270, 719, 932
Sfr274I CTCGAG 1 cut(s) 354
SinI GGWCC 1 cut(s) 782
SlaI CTCGAG 1 cut(s) 354
SmiMI CAYNNNNRTG 2 cut(s) 866, 899
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
Sse9I AATT 9 cut(s) 186, 211, 349, 463, 534, 554, 686, 787, 856
SsiI CCGC 3 cut(s) 233, 695, 701
SspMI CTAG 3 cut(s) 21, 44, 369
StyD4I CCNGG 2 cut(s) 681, 742
TaaI ACNGT 4 cut(s) 201, 432, 624, 755
TaiI ACGT 3 cut(s) 504, 950, 1038
TaqI TCGA 3 cut(s) 355, 906, 1095
TasI AATT 9 cut(s) 186, 211, 349, 463, 534, 554, 686, 787, 856
TatI WGTACW 1 cut(s) 64
TauI GCSGC 2 cut(s) 235, 703
Tru1I TTAA 2 cut(s) 557, 1059
Tru9I TTAA 2 cut(s) 557, 1059
TscAI CASTG 2 cut(s) 627, 850
TseFI GTSAC 1 cut(s) 492
TseI GCWGC 7 cut(s) 269, 272, 437, 578, 697, 770, 934
Tsp45I GTSAC 1 cut(s) 492
TspDTI ATGAA 2 cut(s) 301, 850
TspGWI ACGGA 2 cut(s) 377, 1139
TspRI CASTG 2 cut(s) 627, 850
VpaK11BI GGWCC 1 cut(s) 782
XapI RAATTY 4 cut(s) 186, 211, 686, 856
XceI RCATGY 1 cut(s) 869
XhoI CTCGAG 1 cut(s) 354
XspI CTAG 3 cut(s) 21, 44, 369
ZraI GACGTC 1 cut(s) 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.