Belongs to the AB hydrolase superfamily. Lipase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 49, 182 |
| AclWI | GGATC | 1 cut(s) | 391 |
| AcuI | CTGAAG | 1 cut(s) | 406 |
| AfaI | GTAC | 3 cut(s) | 164, 302, 450 |
| AflII | CTTAAG | 1 cut(s) | 17 |
| AgsI | TTSAA | 2 cut(s) | 11, 417 |
| AjnI | CCWGG | 1 cut(s) | 37 |
| AluBI | AGCT | 1 cut(s) | 289 |
| AluI | AGCT | 1 cut(s) | 289 |
| AlwI | GGATC | 1 cut(s) | 391 |
| AspLEI | GCGC | 1 cut(s) | 226 |
| AsuHPI | GGTGA | 1 cut(s) | 350 |
| BccI | CCATC | 2 cut(s) | 80, 272 |
| BciT130I | CCWGG | 1 cut(s) | 39 |
| BfrI | CTTAAG | 1 cut(s) | 17 |
| Bme1390I | CCNGG | 1 cut(s) | 39 |
| BmrFI | CCNGG | 1 cut(s) | 39 |
| BmsI | GCATC | 1 cut(s) | 211 |
| BpmI | CTGGAG | 1 cut(s) | 141 |
| BsaJI | CCNNGG | 1 cut(s) | 202 |
| BseBI | CCWGG | 1 cut(s) | 39 |
| BseDI | CCNNGG | 1 cut(s) | 202 |
| BseRI | GAGGAG | 1 cut(s) | 137 |
| BsmI | GAATGC | 1 cut(s) | 177 |
| Bsp1407I | TGTACA | 1 cut(s) | 448 |
| Bsp143I | GATC | 2 cut(s) | 271, 396 |
| BspACI | CCGC | 2 cut(s) | 49, 182 |
| BspHI | TCATGA | 1 cut(s) | 274 |
| BspPI | GGATC | 1 cut(s) | 391 |
| BspTI | CTTAAG | 1 cut(s) | 17 |
| BsrGI | TGTACA | 1 cut(s) | 448 |
| BssECI | CCNNGG | 1 cut(s) | 202 |
| BssMI | GATC | 2 cut(s) | 271, 396 |
| Bst2UI | CCWGG | 1 cut(s) | 39 |
| Bst4CI | ACNGT | 3 cut(s) | 76, 143, 510 |
| BstAFI | CTTAAG | 1 cut(s) | 17 |
| BstAUI | TGTACA | 1 cut(s) | 448 |
| BstC8I | GCNNGC | 1 cut(s) | 175 |
| BstHHI | GCGC | 1 cut(s) | 226 |
| BstKTI | GATC | 2 cut(s) | 274, 399 |
| BstMBI | GATC | 2 cut(s) | 271, 396 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 48 |
| BstNI | CCWGG | 1 cut(s) | 39 |
| BstSCI | CCNGG | 1 cut(s) | 37 |
| BtgZI | GCGATG | 1 cut(s) | 113 |
| BtsIMutI | CAGTG | 2 cut(s) | 81, 430 |
| Cac8I | GCNNGC | 1 cut(s) | 175 |
| CciI | TCATGA | 1 cut(s) | 274 |
| CfoI | GCGC | 1 cut(s) | 226 |
| Csp6I | GTAC | 3 cut(s) | 163, 301, 449 |
| CviAII | CATG | 8 cut(s) | 185, 275, 320, 334, 374, 388, 405, 499 |
| CviJI | RGCY | 3 cut(s) | 264, 289, 409 |
| CviKI_1 | RGCY | 3 cut(s) | 264, 289, 409 |
| CviQI | GTAC | 3 cut(s) | 163, 301, 449 |
| DpnI | GATC | 2 cut(s) | 273, 398 |
| DpnII | GATC | 2 cut(s) | 271, 396 |
| Eco57I | CTGAAG | 1 cut(s) | 406 |
| EcoRII | CCWGG | 1 cut(s) | 37 |
| FaeI | CATG | 8 cut(s) | 188, 278, 323, 337, 377, 391, 408, 502 |
| FatI | CATG | 8 cut(s) | 184, 274, 319, 333, 373, 387, 404, 498 |
| GlaI | GCGC | 1 cut(s) | 225 |
| GsuI | CTGGAG | 1 cut(s) | 141 |
| HhaI | GCGC | 1 cut(s) | 226 |
| Hin1II | CATG | 8 cut(s) | 188, 278, 323, 337, 377, 391, 408, 502 |
| Hin6I | GCGC | 1 cut(s) | 224 |
| HinP1I | GCGC | 1 cut(s) | 224 |
| HincII | GTYRAC | 1 cut(s) | 46 |
| HindII | GTYRAC | 1 cut(s) | 46 |
| HindIII | AAGCTT | 1 cut(s) | 287 |
| HinfI | GANTC | 1 cut(s) | 295 |
| HphI | GGTGA | 1 cut(s) | 350 |
| Hpy166II | GTNNAC | 1 cut(s) | 46 |
| Hpy188I | TCNGA | 1 cut(s) | 232 |
| Hpy188III | TCNNGA | 3 cut(s) | 146, 158, 275 |
| Hpy8I | GTNNAC | 1 cut(s) | 46 |
| HpyCH4III | ACNGT | 3 cut(s) | 76, 143, 510 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 48 |
| Hsp92II | CATG | 8 cut(s) | 188, 278, 323, 337, 377, 391, 408, 502 |
| HspAI | GCGC | 1 cut(s) | 224 |
| Kzo9I | GATC | 2 cut(s) | 271, 396 |
| LmnI | GCTCC | 1 cut(s) | 35 |
| LpnPI | CCDG | 4 cut(s) | 24, 51, 131, 171 |
| LweI | GCATC | 1 cut(s) | 211 |
| MalI | GATC | 2 cut(s) | 273, 398 |
| MboI | GATC | 2 cut(s) | 271, 396 |
| MboII | GAAGA | 2 cut(s) | 405, 467 |
| MnlI | CCTC | 9 cut(s) | 76, 115, 165, 204, 206, 212, 275, 410, 451 |
| MseI | TTAA | 1 cut(s) | 18 |
| MslI | CAYNNNNRTG | 1 cut(s) | 392 |
| MspCI | CTTAAG | 1 cut(s) | 17 |
| MspR9I | CCNGG | 1 cut(s) | 39 |
| Mva1269I | GAATGC | 1 cut(s) | 177 |
| MvaI | CCWGG | 1 cut(s) | 39 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 48 |
| NdeII | GATC | 2 cut(s) | 271, 396 |
| NlaIII | CATG | 8 cut(s) | 188, 278, 323, 337, 377, 391, 408, 502 |
| PagI | TCATGA | 1 cut(s) | 274 |
| PctI | GAATGC | 1 cut(s) | 177 |
| PfeI | GAWTC | 1 cut(s) | 295 |
| Psp6I | CCWGG | 1 cut(s) | 37 |
| PspGI | CCWGG | 1 cut(s) | 37 |
| PsrI | GAACNNNNNNTAC | 1 cut(s) | 29 |
| RsaI | GTAC | 3 cut(s) | 164, 302, 450 |
| RsaNI | GTAC | 3 cut(s) | 163, 301, 449 |
| RseI | CAYNNNNRTG | 1 cut(s) | 392 |
| SaqAI | TTAA | 1 cut(s) | 18 |
| Sau3AI | GATC | 2 cut(s) | 271, 396 |
| ScrFI | CCNGG | 1 cut(s) | 39 |
| SetI | ASST | 3 cut(s) | 68, 291, 443 |
| SfaNI | GCATC | 1 cut(s) | 211 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 392 |
| SmlI | CTYRAG | 1 cut(s) | 17 |
| SmoI | CTYRAG | 1 cut(s) | 17 |
| SsiI | CCGC | 2 cut(s) | 49, 182 |
| StyD4I | CCNGG | 1 cut(s) | 37 |
| TaaI | ACNGT | 3 cut(s) | 76, 143, 510 |
| TaqI | TCGA | 1 cut(s) | 105 |
| TatI | WGTACW | 3 cut(s) | 162, 300, 448 |
| TfiI | GAWTC | 1 cut(s) | 295 |
| Tru1I | TTAA | 1 cut(s) | 18 |
| Tru9I | TTAA | 1 cut(s) | 18 |
| TscAI | CASTG | 2 cut(s) | 81, 430 |
| TspRI | CASTG | 2 cut(s) | 81, 430 |
| Vha464I | CTTAAG | 1 cut(s) | 17 |