Belongs to the AB hydrolase superfamily. Lipase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 318 |
| AciI | CCGC | 1 cut(s) | 105 |
| AclWI | GGATC | 2 cut(s) | 262, 275 |
| AcuI | CTGAAG | 1 cut(s) | 9 |
| AfaI | GTAC | 5 cut(s) | 35, 305, 365, 498, 503 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 287, 585 |
| AgsI | TTSAA | 2 cut(s) | 286, 426 |
| AjiI | CACGTC | 2 cut(s) | 436, 445 |
| AluBI | AGCT | 3 cut(s) | 146, 408, 490 |
| AluI | AGCT | 3 cut(s) | 146, 408, 490 |
| AlwI | GGATC | 2 cut(s) | 262, 275 |
| ApeKI | GCWGC | 2 cut(s) | 146, 206 |
| AspLEI | GCGC | 1 cut(s) | 320 |
| AsuC2I | CCSGG | 1 cut(s) | 161 |
| BamHI | GGATCC | 1 cut(s) | 267 |
| BbvCI | CCTCAGC | 1 cut(s) | 404 |
| BbvI | GCAGC | 2 cut(s) | 133, 193 |
| BclI | TGATCA | 1 cut(s) | 43 |
| BcnI | CCSGG | 1 cut(s) | 161 |
| BfaI | CTAG | 1 cut(s) | 572 |
| BisI | GCNGC | 3 cut(s) | 105, 147, 207 |
| BlsI | GCNGC | 3 cut(s) | 106, 148, 208 |
| Bme1390I | CCNGG | 1 cut(s) | 161 |
| BmgBI | CACGTC | 2 cut(s) | 436, 445 |
| BmiI | GGNNCC | 2 cut(s) | 269, 292 |
| BmrFI | CCNGG | 1 cut(s) | 161 |
| Bpu10I | CCTNAGC | 1 cut(s) | 404 |
| BpuMI | CCSGG | 1 cut(s) | 161 |
| BsaJI | CCNNGG | 1 cut(s) | 81 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 107, 137 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 287, 585 |
| Bse1I | ACTGG | 1 cut(s) | 31 |
| BseDI | CCNNGG | 1 cut(s) | 81 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 287, 585 |
| BseMII | CTCAG | 1 cut(s) | 418 |
| BseNI | ACTGG | 1 cut(s) | 31 |
| BseRI | GAGGAG | 1 cut(s) | 345 |
| BseXI | GCAGC | 2 cut(s) | 133, 193 |
| Bsh1236I | CGCG | 1 cut(s) | 318 |
| BsiSI | CCGG | 1 cut(s) | 161 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 287, 585 |
| BsmI | GAATGC | 1 cut(s) | 550 |
| Bsp1407I | TGTACA | 1 cut(s) | 363 |
| Bsp143I | GATC | 5 cut(s) | 13, 43, 140, 267, 472 |
| Bsp19I | CCATGG | 1 cut(s) | 81 |
| BspACI | CCGC | 1 cut(s) | 105 |
| BspCNI | CTCAG | 1 cut(s) | 417 |
| BspFNI | CGCG | 1 cut(s) | 318 |
| BspHI | TCATGA | 2 cut(s) | 475, 534 |
| BspLI | GGNNCC | 2 cut(s) | 269, 292 |
| BspPI | GGATC | 2 cut(s) | 262, 275 |
| BsrGI | TGTACA | 1 cut(s) | 363 |
| BsrI | ACTGG | 1 cut(s) | 31 |
| BssECI | CCNNGG | 1 cut(s) | 81 |
| BssMI | GATC | 5 cut(s) | 13, 43, 140, 267, 472 |
| BssT1I | CCWWGG | 1 cut(s) | 81 |
| Bst4CI | ACNGT | 2 cut(s) | 314, 501 |
| BstAUI | TGTACA | 1 cut(s) | 363 |
| BstDEI | CTNAG | 3 cut(s) | 137, 155, 404 |
| BstDSI | CCRYGG | 1 cut(s) | 81 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 583 |
| BstFNI | CGCG | 1 cut(s) | 318 |
| BstHHI | GCGC | 1 cut(s) | 320 |
| BstKTI | GATC | 5 cut(s) | 16, 46, 143, 270, 475 |
| BstMBI | GATC | 5 cut(s) | 13, 43, 140, 267, 472 |
| BstSCI | CCNGG | 1 cut(s) | 159 |
| BstUI | CGCG | 1 cut(s) | 318 |
| BstV1I | GCAGC | 2 cut(s) | 133, 193 |
| BstX2I | RGATCY | 1 cut(s) | 267 |
| BstYI | RGATCY | 1 cut(s) | 267 |
| BtgI | CCRYGG | 1 cut(s) | 81 |
| BtrI | CACGTC | 2 cut(s) | 436, 445 |
| BtsIMutI | CAGTG | 1 cut(s) | 188 |
| CciI | TCATGA | 2 cut(s) | 475, 534 |
| CfoI | GCGC | 1 cut(s) | 320 |
| Csp6I | GTAC | 5 cut(s) | 34, 304, 364, 497, 502 |
| CviAII | CATG | 6 cut(s) | 82, 325, 331, 370, 476, 535 |
| CviJI | RGCY | 7 cut(s) | 146, 159, 349, 408, 490, 578, 589 |
| CviKI_1 | RGCY | 7 cut(s) | 146, 159, 349, 408, 490, 578, 589 |
| CviQI | GTAC | 5 cut(s) | 34, 304, 364, 497, 502 |
| DdeI | CTNAG | 3 cut(s) | 137, 155, 404 |
| DpnI | GATC | 5 cut(s) | 15, 45, 142, 269, 474 |
| DpnII | GATC | 5 cut(s) | 13, 43, 140, 267, 472 |
| Eco130I | CCWWGG | 1 cut(s) | 81 |
| Eco57I | CTGAAG | 1 cut(s) | 9 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 583 |
| EcoT14I | CCWWGG | 1 cut(s) | 81 |
| ErhI | CCWWGG | 1 cut(s) | 81 |
| FaeI | CATG | 6 cut(s) | 85, 328, 334, 373, 479, 538 |
| FatI | CATG | 6 cut(s) | 81, 324, 330, 369, 475, 534 |
| FbaI | TGATCA | 1 cut(s) | 43 |
| Fnu4HI | GCNGC | 3 cut(s) | 105, 147, 207 |
| Fsp4HI | GCNGC | 3 cut(s) | 105, 147, 207 |
| FspBI | CTAG | 1 cut(s) | 572 |
| GlaI | GCGC | 1 cut(s) | 319 |
| GluI | GCNGC | 3 cut(s) | 105, 147, 207 |
| HapII | CCGG | 1 cut(s) | 161 |
| HhaI | GCGC | 1 cut(s) | 320 |
| Hin1II | CATG | 6 cut(s) | 85, 328, 334, 373, 479, 538 |
| Hin6I | GCGC | 1 cut(s) | 318 |
| HinP1I | GCGC | 1 cut(s) | 318 |
| HindIII | AAGCTT | 1 cut(s) | 488 |
| HinfI | GANTC | 1 cut(s) | 422 |
| HpaII | CCGG | 1 cut(s) | 161 |
| Hpy188I | TCNGA | 3 cut(s) | 74, 115, 433 |
| Hpy188III | TCNNGA | 3 cut(s) | 476, 535, 572 |
| Hpy99I | CGWCG | 2 cut(s) | 74, 437 |
| HpyAV | CCTTC | 2 cut(s) | 118, 589 |
| HpyCH4III | ACNGT | 2 cut(s) | 314, 501 |
| HpyCH4IV | ACGT | 4 cut(s) | 36, 69, 435, 444 |
| HpyF3I | CTNAG | 3 cut(s) | 137, 155, 404 |
| HpySE526I | ACGT | 4 cut(s) | 36, 69, 435, 444 |
| Hsp92II | CATG | 6 cut(s) | 85, 328, 334, 373, 479, 538 |
| HspAI | GCGC | 1 cut(s) | 318 |
| Ksp22I | TGATCA | 1 cut(s) | 43 |
| Kzo9I | GATC | 5 cut(s) | 13, 43, 140, 267, 472 |
| LpnPI | CCDG | 5 cut(s) | 36, 44, 158, 174, 571 |
| Lsp1109I | GCAGC | 2 cut(s) | 133, 193 |
| MaeI | CTAG | 1 cut(s) | 572 |
| MaeII | ACGT | 4 cut(s) | 36, 69, 435, 444 |
| MaeIII | GTNAC | 1 cut(s) | 229 |
| MalI | GATC | 5 cut(s) | 15, 45, 142, 269, 474 |
| MboI | GATC | 5 cut(s) | 13, 43, 140, 267, 472 |
| MboII | GAAGA | 2 cut(s) | 8, 482 |
| MflI | RGATCY | 1 cut(s) | 267 |
| MluCI | AATT | 2 cut(s) | 59, 426 |
| MmeI | TCCRAC | 2 cut(s) | 52, 456 |
| MnlI | CCTC | 9 cut(s) | 110, 196, 366, 405, 407, 413, 476, 502, 579 |
| MseI | TTAA | 5 cut(s) | 129, 197, 240, 273, 595 |
| MslI | CAYNNNNRTG | 3 cut(s) | 299, 329, 539 |
| MspA1I | CMGCKG | 1 cut(s) | 146 |
| MspI | CCGG | 1 cut(s) | 161 |
| MspR9I | CCNGG | 1 cut(s) | 161 |
| Mva1269I | GAATGC | 1 cut(s) | 550 |
| MvnI | CGCG | 1 cut(s) | 318 |
| NciI | CCSGG | 1 cut(s) | 161 |
| NcoI | CCATGG | 1 cut(s) | 81 |
| NdeII | GATC | 5 cut(s) | 13, 43, 140, 267, 472 |
| NlaIII | CATG | 6 cut(s) | 85, 328, 334, 373, 479, 538 |
| NlaIV | GGNNCC | 2 cut(s) | 269, 292 |
| PagI | TCATGA | 2 cut(s) | 475, 534 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 441 |
| PctI | GAATGC | 1 cut(s) | 550 |
| PfeI | GAWTC | 1 cut(s) | 422 |
| PkrI | GCNGC | 3 cut(s) | 106, 148, 208 |
| PspN4I | GGNNCC | 2 cut(s) | 269, 292 |
| PsuI | RGATCY | 1 cut(s) | 267 |
| PvuII | CAGCTG | 1 cut(s) | 146 |
| RsaI | GTAC | 5 cut(s) | 35, 305, 365, 498, 503 |
| RsaNI | GTAC | 5 cut(s) | 34, 304, 364, 497, 502 |
| RseI | CAYNNNNRTG | 3 cut(s) | 299, 329, 539 |
| SaqAI | TTAA | 5 cut(s) | 129, 197, 240, 273, 595 |
| SatI | GCNGC | 3 cut(s) | 105, 147, 207 |
| Sau3AI | GATC | 5 cut(s) | 13, 43, 140, 267, 472 |
| ScrFI | CCNGG | 1 cut(s) | 161 |
| SetI | ASST | 9 cut(s) | 39, 72, 148, 250, 410, 438, 447, 468, 492 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 299, 329, 539 |
| Sse9I | AATT | 2 cut(s) | 59, 426 |
| SsiI | CCGC | 1 cut(s) | 105 |
| SspMI | CTAG | 1 cut(s) | 572 |
| StyD4I | CCNGG | 1 cut(s) | 159 |
| StyI | CCWWGG | 1 cut(s) | 81 |
| TaaI | ACNGT | 2 cut(s) | 314, 501 |
| TaiI | ACGT | 4 cut(s) | 39, 72, 438, 447 |
| TaqI | TCGA | 1 cut(s) | 468 |
| TasI | AATT | 2 cut(s) | 59, 426 |
| TatI | WGTACW | 2 cut(s) | 363, 501 |
| TauI | GCSGC | 1 cut(s) | 107 |
| TfiI | GAWTC | 1 cut(s) | 422 |
| Tru1I | TTAA | 5 cut(s) | 129, 197, 240, 273, 595 |
| Tru9I | TTAA | 5 cut(s) | 129, 197, 240, 273, 595 |
| TscAI | CASTG | 1 cut(s) | 195 |
| TseI | GCWGC | 2 cut(s) | 146, 206 |
| TspDTI | ATGAA | 4 cut(s) | 258, 341, 571, 581 |
| TspGWI | ACGGA | 1 cut(s) | 29 |
| TspRI | CASTG | 1 cut(s) | 195 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 583 |
| XbaI | TCTAGA | 1 cut(s) | 571 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 88 |
| XspI | CTAG | 1 cut(s) | 572 |