MD17G1107600.v1.1
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000706
Physical Location & Seq
Forward (+)
9251556 .. 9253730
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1107600.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGGAAAATCCTTCAACCACTATACTTGTTGTGGCTTTACTTTGGGTTTCAGTAGTTGCAGCAACAAGAACTAAGCTGCAATTGATTGACGACGCTGATGGTGGCATTTGCAAATCAATGGTGGAGACACAAGGCTATACTTGCCAAGAACATAAAGTAACAGCCAGAGACGGCTACATCCTCGGCTTGCACAGAATTCCGAAGGGACGGTCTGGTAATAAGACGCCAAACAAGGAGCTGCCTGTTTTGTTACAACATGGGCTTTTAATGGATGCGTCAGCATGGCTACTCAATCCGCAAGATCAAGCTTTGGCATTTATCTTGGCAGACAATGGTTTCGATGTTTGGCTTGCCAACACTCGCGGGACAAATTCTAGTCGCGGACATACATTACTTACTCCTAATAATCCTGTTCGTGCTTACTGGGACTGGTCATGGGATGAATTGGCTGCTTATGATCTTCCTGCTTTATTCGAGTATGTGAACAATCAAACAGGACAGAAATTACACTATGTTGGTCACTCCTTGGGAACTTTGACTGCCCTTGCTGCCTTCTCTCAAGAAAATCTGTTAAACTTGCTAAGATCAGCTGCTTTGCTTAGCCCGGTTGCTTACATGGGTCAGATGTCATCGCTGTTTCTAAGGATTCTTATGAACATATTTTTAGCAGAAGTAACACAAATCAGCGCCTACTCTTTTGCTTTAAAAAATATGGGCCCAAATTGCTGCCTCAGTTCTGCAAGCAAAGCTACTTTGTTTAAGAATTTTCCCCAGTCCACGGCGACAAAGAACATGATACATCTGTCTCAGATGGTCAGGATGGGAACCCTAGAAATGTACGATTATGGTCTAGAAGACGCGAACATGGAACACTACAAGCAGCCTACTCCTCCTGTGTACAACATGGCGAACATTCCGAAAGATGTTCCTCTTTTCCTCAGCTACGGGGGGAGAGATGCGATTTCTGATGTTAATGACGTGGGGCTTCTGCTTGACAACCTCAAAGATCATGACAAGGACAAGCTTGTGGTAGAGTACAGAGAGGATTATGCTCATCTGGATTTTATTATGAGTGTGAATGCCAATCAACTTGTGTATAATCCCATACTGGCCTTCTTCAAGCTCCATTAA

Protein Analysis

377

Amino Acids

41.95

Weight (kDa)

5.86

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 39 - 98 1.2e-19 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 81 - 219 2e-11 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 82 - 247 3.1e-06 Alpha/beta hydrolase family
Hydrolase_4 PF12146 102 - 339 4.1e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000706)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03230 FvH4_5g03471 FvH4_5g03472 FvH4_6g41950 FvH4_6g41951
malus_domestica MD09G1115700.v1.1 MD09G1115900.v1.1 MD17G1107600.v1.1 MD17G1107700.v1.1
prunus_persica Prupe.3G208700_v2.0.a1 Prupe.3G208800_v2.0.a1 Prupe.5G037500_v2.0.a1
pyrus_communis pycom09g04010 pycom09g04030 pycom17g10020 pycom17g10030
rosa_chinensis RchiOBHm_Chr2g0101861 RchiOBHm_Chr2g0157451 RchiOBHm_Chr2g0157461 RchiOBHm_Chr2g0157841 RchiOBHm_Chr2g0157851 RchiOBHm_Chr3g0484721 RchiOBHm_Chr3g0484731 RchiOBHm_Chr3g0484741 RchiOBHm_Chr3g0484751 RchiOBHm_Chr6g0253931 RchiOBHm_Chr6g0253991
rosa_laevigata RLG00000014950 RLG00000014954 RLG00000020989 RLG00000021024 RLG00000021026 RLG00000021027 RLG00000021029 RLG00000021030
rosa_multiflora Rmu_sc0000195.1_g000023 Rmu_sc0000195.1_g000028 Rmu_sc0001689.1_g000007 Rmu_sc0001716.1_g000018 Rmu_sc0002441.1_g000012 Rmu_sc0004191.1_g000065 Rmu_sc0004473.1_g000004
rosa_roxburghii Rroxscaffold_1G00036760 Rroxscaffold_1G00036780 Rroxscaffold_2G00091900 Rroxscaffold_2G00092180 Rroxscaffold_2G00092200 Rroxscaffold_2G00127230 Rroxscaffold_7G00211340 Rroxscaffold_7G00211380
rosa_rugosa Rorug02G0102500 Rorug02G0206400 Rorug02G0206500 Rorug02G0461400 Rorug02G0464100 Rorug05G0548600 Rorug05G0548800
rosa_samantha Rh2BG155500 Rh2BG540100 Rh2BG540200 Rh2BG543500 Rh2BG543700 Rh2BG543800 Rh3CG296800 Rh6AG064700 Rh6AG065300
rosa_wichuraiana Rw2G020570 Rw2G043520 Rw2G043530 Rw2G043770 Rw2G043780 Rw3G023530 Rw3G023540 Rw6G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 363, 381, 860
AciI CCGC 3 cut(s) 296, 363, 381
AcsI RAATTY 3 cut(s) 195, 370, 763
AcyI GRCGYC 1 cut(s) 224
AfaI GTAC 3 cut(s) 839, 899, 1037
AfiI CCNNNNNNNGG 1 cut(s) 778
AgsI TTSAA 2 cut(s) 15, 1120
AjiI CACGTC 1 cut(s) 979
AluBI AGCT 8 cut(s) 76, 238, 308, 590, 749, 942, 1024, 1123
AluI AGCT 8 cut(s) 76, 238, 308, 590, 749, 942, 1024, 1123
Alw26I GTCTC 3 cut(s) 119, 162, 810
AoxI GGCC 2 cut(s) 715, 1110
ApaI GGGCCC 1 cut(s) 719
ApeKI GCWGC 8 cut(s) 59, 76, 238, 449, 548, 590, 726, 880
ApoI RAATTY 3 cut(s) 195, 370, 763
ArsI GACNNNNNNTTYG 4 cut(s) 194, 226, 320, 352
AspLEI GCGC 1 cut(s) 689
AspS9I GGNCC 2 cut(s) 715, 716
AsuC2I CCSGG 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 719
BaeI ACNNNNGTAYC 2 cut(s) 788, 821
BanII GRGCYC 1 cut(s) 719
BbsI GAAGAC 1 cut(s) 861
BbvCI CCTCAGC 1 cut(s) 938
BbvI GCAGC 8 cut(s) 63, 71, 225, 436, 535, 577, 713, 892
BccI CCATC 3 cut(s) 92, 805, 814
BceAI ACGGC 2 cut(s) 187, 795
BcnI CCSGG 1 cut(s) 605
BcoDI GTCTC 3 cut(s) 119, 162, 810
BfaI CTAG 3 cut(s) 375, 830, 851
BfoI RGCGCY 1 cut(s) 690
BisI GCNGC 8 cut(s) 60, 77, 239, 450, 549, 591, 727, 881
BlpI GCTNAGC 1 cut(s) 599
BlsI GCNGC 8 cut(s) 61, 78, 240, 451, 550, 592, 728, 882
Bme1390I CCNGG 1 cut(s) 605
BmgBI CACGTC 1 cut(s) 979
BmgT120I GGNCC 2 cut(s) 715, 716
BmiI GGNNCC 2 cut(s) 717, 826
BmrFI CCNGG 1 cut(s) 605
BmrI ACTGGG 2 cut(s) 433, 766
BmsI GCATC 2 cut(s) 262, 946
BmuI ACTGGG 2 cut(s) 433, 766
BpiI GAAGAC 1 cut(s) 861
Bpu10I CCTNAGC 1 cut(s) 938
Bpu1102I GCTNAGC 1 cut(s) 599
BpuEI CTTGAG 1 cut(s) 543
BpuMI CCSGG 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 181, 525, 777
Bsc4I CCNNNNNNNGG 1 cut(s) 778
Bse1I ACTGG 4 cut(s) 428, 434, 772, 1113
BseDI CCNNGG 3 cut(s) 181, 525, 777
BseGI GGATG 4 cut(s) 177, 277, 445, 825
BseLI CCNNNNNNNGG 1 cut(s) 778
BseMII CTCAG 3 cut(s) 745, 821, 952
BseNI ACTGG 4 cut(s) 428, 434, 772, 1113
BseRI GAGGAG 1 cut(s) 879
BseSI GKGCMC 1 cut(s) 719
BseXI GCAGC 8 cut(s) 63, 71, 225, 436, 535, 577, 713, 892
Bsh1236I CGCG 3 cut(s) 363, 381, 860
BshFI GGCC 2 cut(s) 717, 1112
BsiSI CCGG 1 cut(s) 605
BslFI GGGAC 3 cut(s) 219, 379, 440
BslI CCNNNNNNNGG 1 cut(s) 778
BsmAI GTCTC 3 cut(s) 119, 162, 810
BsmBI CGTCTC 1 cut(s) 162
BsmFI GGGAC 3 cut(s) 219, 379, 440
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 2 cut(s) 717, 1112
Bsp120I GGGCCC 1 cut(s) 715
Bsp1286I GDGCHC 1 cut(s) 719
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 4 cut(s) 301, 457, 584, 1006
Bsp1720I GCTNAGC 1 cut(s) 599
BspACI CCGC 3 cut(s) 296, 363, 381
BspANI GGCC 2 cut(s) 717, 1112
BspCNI CTCAG 3 cut(s) 744, 820, 951
BspFNI CGCG 3 cut(s) 363, 381, 860
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 2 cut(s) 717, 826
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 4 cut(s) 428, 434, 772, 1113
BssECI CCNNGG 3 cut(s) 181, 525, 777
BssMI GATC 4 cut(s) 301, 457, 584, 1006
BssNI GRCGYC 1 cut(s) 224
BssT1I CCWWGG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 224
BstAUI TGTACA 1 cut(s) 897
BstC8I GCNNGC 3 cut(s) 188, 351, 742
BstDEI CTNAG 7 cut(s) 72, 581, 599, 641, 731, 807, 938
BstDSI CCRYGG 1 cut(s) 777
BstF5I GGATG 4 cut(s) 177, 277, 445, 825
BstFNI CGCG 3 cut(s) 363, 381, 860
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 1 cut(s) 689
BstKTI GATC 4 cut(s) 304, 460, 587, 1009
BstMAI GTCTC 3 cut(s) 119, 162, 810
BstMBI GATC 4 cut(s) 301, 457, 584, 1006
BstMWI GCNNNNNNNGC 4 cut(s) 141, 548, 723, 746
BstSCI CCNGG 1 cut(s) 603
BstSLI GKGCMC 1 cut(s) 719
BstUI CGCG 3 cut(s) 363, 381, 860
BstV1I GCAGC 8 cut(s) 63, 71, 225, 436, 535, 577, 713, 892
BstV2I GAAGAC 1 cut(s) 861
BsuRI GGCC 2 cut(s) 717, 1112
BtgI CCRYGG 1 cut(s) 777
BtgZI GCGATG 1 cut(s) 615
BtrI CACGTC 1 cut(s) 979
BtsCI GGATG 4 cut(s) 177, 277, 445, 825
Cac8I GCNNGC 3 cut(s) 188, 351, 742
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 1 cut(s) 689
Cfr13I GGNCC 2 cut(s) 715, 716
CseI GACGC 4 cut(s) 101, 232, 264, 866
Csp6I GTAC 3 cut(s) 838, 898, 1036
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 8 cut(s) 257, 282, 435, 616, 793, 865, 904, 1010
CviQI GTAC 3 cut(s) 838, 898, 1036
DdeI CTNAG 7 cut(s) 72, 581, 599, 641, 731, 807, 938
DpnI GATC 4 cut(s) 303, 459, 586, 1008
DpnII GATC 4 cut(s) 301, 457, 584, 1006
DraI TTTAAA 1 cut(s) 705
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 719
EcoRI GAATTC 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 719
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 8 cut(s) 260, 285, 438, 619, 796, 868, 907, 1013
FaqI GGGAC 3 cut(s) 219, 379, 440
FatI CATG 8 cut(s) 256, 281, 434, 615, 792, 864, 903, 1009
FauI CCCGC 1 cut(s) 356
Fnu4HI GCNGC 8 cut(s) 60, 77, 239, 450, 549, 591, 727, 881
FokI GGATG 4 cut(s) 164, 284, 452, 832
FriOI GRGCYC 1 cut(s) 719
Fsp4HI GCNGC 8 cut(s) 60, 77, 239, 450, 549, 591, 727, 881
FspBI CTAG 3 cut(s) 375, 830, 851
GlaI GCGC 1 cut(s) 688
GluI GCNGC 8 cut(s) 60, 77, 239, 450, 549, 591, 727, 881
HaeII RGCGCY 1 cut(s) 690
HaeIII GGCC 2 cut(s) 717, 1112
HapII CCGG 1 cut(s) 605
HgaI GACGC 4 cut(s) 101, 232, 264, 866
HhaI GCGC 1 cut(s) 689
Hin1I GRCGYC 1 cut(s) 224
Hin1II CATG 8 cut(s) 260, 285, 438, 619, 796, 868, 907, 1013
Hin6I GCGC 1 cut(s) 687
HinP1I GCGC 1 cut(s) 687
HindIII AAGCTT 2 cut(s) 306, 1022
HinfI GANTC 1 cut(s) 646
HpaII CCGG 1 cut(s) 605
Hpy166II GTNNAC 3 cut(s) 484, 777, 898
Hpy188I TCNGA 5 cut(s) 201, 624, 810, 918, 967
Hpy188III TCNNGA 5 cut(s) 560, 817, 851, 1010, 1058
Hpy8I GTNNAC 3 cut(s) 484, 777, 898
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 4 cut(s) 21, 196, 562, 1123
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4IV ACGT 1 cut(s) 978
HpyCH4V TGCA 5 cut(s) 59, 79, 111, 190, 740
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 548, 723, 746
HpyF3I CTNAG 7 cut(s) 72, 581, 599, 641, 731, 807, 938
HpySE526I ACGT 1 cut(s) 978
Hsp92I GRCGYC 1 cut(s) 224
Hsp92II CATG 8 cut(s) 260, 285, 438, 619, 796, 868, 907, 1013
HspAI GCGC 1 cut(s) 687
Kzo9I GATC 4 cut(s) 301, 457, 584, 1006
LmnI GCTCC 2 cut(s) 235, 1128
Lsp1109I GCAGC 8 cut(s) 63, 71, 225, 436, 535, 577, 713, 892
LweI GCATC 2 cut(s) 262, 946
MaeI CTAG 3 cut(s) 375, 830, 851
MaeII ACGT 1 cut(s) 978
MaeIII GTNAC 4 cut(s) 157, 249, 518, 673
MalI GATC 4 cut(s) 303, 459, 586, 1008
MboI GATC 4 cut(s) 301, 457, 584, 1006
MboII GAAGA 3 cut(s) 452, 866, 1108
MfeI CAATTG 1 cut(s) 80
MhlI GDGCHC 1 cut(s) 719
MluCI AATT 7 cut(s) 80, 195, 370, 443, 503, 721, 763
MnlI CCTC 7 cut(s) 191, 740, 900, 939, 947, 1010, 1036
MseI TTAA 6 cut(s) 266, 572, 704, 759, 972, 1129
MspA1I CMGCKG 1 cut(s) 590
MspI CCGG 1 cut(s) 605
MspR9I CCNGG 1 cut(s) 605
MunI CAATTG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 1084
MvnI CGCG 3 cut(s) 363, 381, 860
MwoI GCNNNNNNNGC 4 cut(s) 141, 548, 723, 746
NciI CCSGG 1 cut(s) 605
NdeII GATC 4 cut(s) 301, 457, 584, 1006
NlaIII CATG 8 cut(s) 260, 285, 438, 619, 796, 868, 907, 1013
NlaIV GGNNCC 2 cut(s) 717, 826
NmeAIII GCCGAG 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 518
PagI TCATGA 1 cut(s) 1009
PctI GAATGC 1 cut(s) 1084
PfeI GAWTC 1 cut(s) 646
PkrI GCNGC 8 cut(s) 61, 78, 240, 451, 550, 592, 728, 882
PspN4I GGNNCC 2 cut(s) 717, 826
PspOMI GGGCCC 1 cut(s) 715
PspPI GGNCC 2 cut(s) 715, 716
PvuII CAGCTG 1 cut(s) 590
RsaI GTAC 3 cut(s) 839, 899, 1037
RsaNI GTAC 3 cut(s) 838, 898, 1036
SaqAI TTAA 6 cut(s) 266, 572, 704, 759, 972, 1129
SatI GCNGC 8 cut(s) 60, 77, 239, 450, 549, 591, 727, 881
Sau3AI GATC 4 cut(s) 301, 457, 584, 1006
Sau96I GGNCC 2 cut(s) 715, 716
ScrFI CCNGG 1 cut(s) 605
SduI GDGCHC 1 cut(s) 719
SfaNI GCATC 2 cut(s) 262, 946
SmlI CTYRAG 1 cut(s) 558
SmoI CTYRAG 1 cut(s) 558
Sse9I AATT 7 cut(s) 80, 195, 370, 443, 503, 721, 763
SsiI CCGC 3 cut(s) 296, 363, 381
SspMI CTAG 3 cut(s) 375, 830, 851
StyD4I CCNGG 1 cut(s) 603
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 1 cut(s) 210
TaiI ACGT 1 cut(s) 981
TaqI TCGA 2 cut(s) 339, 474
TasI AATT 7 cut(s) 80, 195, 370, 443, 503, 721, 763
TatI WGTACW 2 cut(s) 897, 1035
TfiI GAWTC 1 cut(s) 646
Tru1I TTAA 6 cut(s) 266, 572, 704, 759, 972, 1129
Tru9I TTAA 6 cut(s) 266, 572, 704, 759, 972, 1129
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 8 cut(s) 59, 76, 238, 449, 548, 590, 726, 880
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 2 cut(s) 456, 668
XapI RAATTY 3 cut(s) 195, 370, 763
XbaI TCTAGA 1 cut(s) 850
XspI CTAG 3 cut(s) 375, 830, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.