Belongs to the AB hydrolase superfamily. Lipase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 290, 374 |
| AcsI | RAATTY | 1 cut(s) | 35 |
| AfaI | GTAC | 3 cut(s) | 170, 230, 380 |
| AgsI | TTSAA | 3 cut(s) | 34, 72, 451 |
| AluBI | AGCT | 3 cut(s) | 273, 355, 454 |
| AluI | AGCT | 3 cut(s) | 273, 355, 454 |
| Alw26I | GTCTC | 1 cut(s) | 279 |
| AoxI | GGCC | 1 cut(s) | 47 |
| ApeKI | GCWGC | 1 cut(s) | 57 |
| ApoI | RAATTY | 1 cut(s) | 35 |
| AspS9I | GGNCC | 1 cut(s) | 47 |
| AsuII | TTCGAA | 1 cut(s) | 296 |
| BbvI | GCAGC | 1 cut(s) | 44 |
| BccI | CCATC | 1 cut(s) | 136 |
| BcoDI | GTCTC | 1 cut(s) | 279 |
| BisI | GCNGC | 1 cut(s) | 58 |
| BlsI | GCNGC | 1 cut(s) | 59 |
| BmgT120I | GGNCC | 1 cut(s) | 47 |
| BmrI | ACTGGG | 1 cut(s) | 97 |
| BmsI | GCATC | 1 cut(s) | 139 |
| BmuI | ACTGGG | 1 cut(s) | 97 |
| Bpu10I | CCTNAGC | 1 cut(s) | 269 |
| Bpu14I | TTCGAA | 1 cut(s) | 296 |
| BsaI | GGTCTC | 1 cut(s) | 279 |
| BsaJI | CCNNGG | 1 cut(s) | 61 |
| Bse1I | ACTGG | 2 cut(s) | 103, 444 |
| Bse3DI | GCAATG | 1 cut(s) | 171 |
| BseDI | CCNNGG | 1 cut(s) | 61 |
| BseGI | GGATG | 1 cut(s) | 243 |
| BseMI | GCAATG | 1 cut(s) | 171 |
| BseMII | CTCAG | 2 cut(s) | 346, 396 |
| BseNI | ACTGG | 2 cut(s) | 103, 444 |
| BseRI | GAGGAG | 1 cut(s) | 210 |
| BseXI | GCAGC | 1 cut(s) | 44 |
| BshFI | GGCC | 1 cut(s) | 49 |
| BsmAI | GTCTC | 1 cut(s) | 279 |
| BsmI | GAATGC | 2 cut(s) | 127, 415 |
| BsnI | GGCC | 1 cut(s) | 49 |
| Bso31I | GGTCTC | 1 cut(s) | 279 |
| Bsp119I | TTCGAA | 1 cut(s) | 296 |
| Bsp1407I | TGTACA | 1 cut(s) | 228 |
| Bsp143I | GATC | 1 cut(s) | 337 |
| BspACI | CCGC | 2 cut(s) | 290, 374 |
| BspANI | GGCC | 1 cut(s) | 49 |
| BspCNI | CTCAG | 2 cut(s) | 345, 397 |
| BspHI | TCATGA | 1 cut(s) | 340 |
| BspT104I | TTCGAA | 1 cut(s) | 296 |
| BspTNI | GGTCTC | 1 cut(s) | 279 |
| BsrDI | GCAATG | 1 cut(s) | 171 |
| BsrGI | TGTACA | 1 cut(s) | 228 |
| BsrI | ACTGG | 2 cut(s) | 103, 444 |
| BssECI | CCNNGG | 1 cut(s) | 61 |
| BssMI | GATC | 1 cut(s) | 337 |
| BstAUI | TGTACA | 1 cut(s) | 228 |
| BstBI | TTCGAA | 1 cut(s) | 296 |
| BstDEI | CTNAG | 3 cut(s) | 269, 332, 405 |
| BstF5I | GGATG | 1 cut(s) | 243 |
| BstKTI | GATC | 1 cut(s) | 340 |
| BstMAI | GTCTC | 1 cut(s) | 279 |
| BstMBI | GATC | 1 cut(s) | 337 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 380, 410 |
| BstV1I | GCAGC | 1 cut(s) | 44 |
| BsuRI | GGCC | 1 cut(s) | 49 |
| BtsCI | GGATG | 1 cut(s) | 243 |
| CciI | TCATGA | 1 cut(s) | 340 |
| Cfr13I | GGNCC | 1 cut(s) | 47 |
| Csp6I | GTAC | 3 cut(s) | 169, 229, 379 |
| CviAII | CATG | 2 cut(s) | 235, 341 |
| CviJI | RGCY | 6 cut(s) | 49, 214, 273, 355, 404, 454 |
| CviKI_1 | RGCY | 6 cut(s) | 49, 214, 273, 355, 404, 454 |
| CviQI | GTAC | 3 cut(s) | 169, 229, 379 |
| DdeI | CTNAG | 3 cut(s) | 269, 332, 405 |
| DpnI | GATC | 1 cut(s) | 339 |
| DpnII | GATC | 1 cut(s) | 337 |
| Eco31I | GGTCTC | 1 cut(s) | 279 |
| EcoT22I | ATGCAT | 1 cut(s) | 132 |
| FaeI | CATG | 2 cut(s) | 238, 344 |
| FatI | CATG | 2 cut(s) | 234, 340 |
| FauI | CCCGC | 1 cut(s) | 297 |
| FauNDI | CATATG | 1 cut(s) | 387 |
| Fnu4HI | GCNGC | 1 cut(s) | 58 |
| FokI | GGATG | 1 cut(s) | 230 |
| Fsp4HI | GCNGC | 1 cut(s) | 58 |
| GluI | GCNGC | 1 cut(s) | 58 |
| HaeIII | GGCC | 1 cut(s) | 49 |
| Hin1II | CATG | 2 cut(s) | 238, 344 |
| HindIII | AAGCTT | 1 cut(s) | 353 |
| Hpy166II | GTNNAC | 2 cut(s) | 108, 229 |
| Hpy188I | TCNGA | 2 cut(s) | 141, 335 |
| Hpy188III | TCNNGA | 2 cut(s) | 247, 341 |
| Hpy8I | GTNNAC | 2 cut(s) | 108, 229 |
| HpyAV | CCTTC | 1 cut(s) | 33 |
| HpyCH4IV | ACGT | 2 cut(s) | 255, 300 |
| HpyCH4V | TGCA | 1 cut(s) | 130 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 380, 410 |
| HpyF3I | CTNAG | 3 cut(s) | 269, 332, 405 |
| HpySE526I | ACGT | 2 cut(s) | 255, 300 |
| Hsp92II | CATG | 2 cut(s) | 238, 344 |
| Kzo9I | GATC | 1 cut(s) | 337 |
| LmnI | GCTCC | 2 cut(s) | 436, 459 |
| LpnPI | CCDG | 5 cut(s) | 116, 198, 237, 308, 425 |
| Lsp1109I | GCAGC | 1 cut(s) | 44 |
| LweI | GCATC | 1 cut(s) | 139 |
| MaeII | ACGT | 2 cut(s) | 255, 300 |
| MaeIII | GTNAC | 1 cut(s) | 145 |
| MalI | GATC | 1 cut(s) | 339 |
| MboI | GATC | 1 cut(s) | 337 |
| MboII | GAAGA | 3 cut(s) | 60, 80, 439 |
| MluCI | AATT | 2 cut(s) | 35, 52 |
| MnlI | CCTC | 5 cut(s) | 71, 231, 270, 272, 341 |
| Mph1103I | ATGCAT | 1 cut(s) | 132 |
| MslI | CAYNNNNRTG | 1 cut(s) | 164 |
| MspA1I | CMGCKG | 1 cut(s) | 374 |
| Mva1269I | GAATGC | 2 cut(s) | 127, 415 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 380, 410 |
| NdeI | CATATG | 1 cut(s) | 387 |
| NdeII | GATC | 1 cut(s) | 337 |
| NlaIII | CATG | 2 cut(s) | 238, 344 |
| NmuCI | GTSAC | 1 cut(s) | 145 |
| NsiI | ATGCAT | 1 cut(s) | 132 |
| NspV | TTCGAA | 1 cut(s) | 296 |
| PagI | TCATGA | 1 cut(s) | 340 |
| PctI | GAATGC | 2 cut(s) | 127, 415 |
| PkrI | GCNGC | 1 cut(s) | 59 |
| PspPI | GGNCC | 1 cut(s) | 47 |
| RsaI | GTAC | 3 cut(s) | 170, 230, 380 |
| RsaNI | GTAC | 3 cut(s) | 169, 229, 379 |
| RseI | CAYNNNNRTG | 1 cut(s) | 164 |
| SatI | GCNGC | 1 cut(s) | 58 |
| Sau3AI | GATC | 1 cut(s) | 337 |
| Sau96I | GGNCC | 1 cut(s) | 47 |
| SetI | ASST | 6 cut(s) | 258, 275, 303, 333, 357, 456 |
| SfaNI | GCATC | 1 cut(s) | 139 |
| SfuI | TTCGAA | 1 cut(s) | 296 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 164 |
| Sse9I | AATT | 2 cut(s) | 35, 52 |
| SsiI | CCGC | 2 cut(s) | 290, 374 |
| TaiI | ACGT | 2 cut(s) | 258, 303 |
| TaqI | TCGA | 3 cut(s) | 91, 113, 296 |
| TasI | AATT | 2 cut(s) | 35, 52 |
| TatI | WGTACW | 1 cut(s) | 228 |
| TseFI | GTSAC | 1 cut(s) | 145 |
| TseI | GCWGC | 1 cut(s) | 57 |
| Tsp45I | GTSAC | 1 cut(s) | 145 |
| XapI | RAATTY | 1 cut(s) | 35 |
| Zsp2I | ATGCAT | 1 cut(s) | 132 |