Belongs to the AB hydrolase superfamily. Lipase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 138 |
| AccI | GTMKAC | 1 cut(s) | 261 |
| AclWI | GGATC | 1 cut(s) | 260 |
| AcoI | YGGCCR | 1 cut(s) | 21 |
| AcyI | GRCGYC | 1 cut(s) | 135 |
| AfaI | GTAC | 1 cut(s) | 65 |
| AluBI | AGCT | 2 cut(s) | 49, 190 |
| AluI | AGCT | 2 cut(s) | 49, 190 |
| AlwI | GGATC | 1 cut(s) | 260 |
| AoxI | GGCC | 1 cut(s) | 21 |
| AsuII | TTCGAA | 1 cut(s) | 83 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 63, 97 |
| BclI | TGATCA | 1 cut(s) | 172 |
| BmsI | GCATC | 1 cut(s) | 112 |
| Bpu14I | TTCGAA | 1 cut(s) | 83 |
| BsaAI | YACGTR | 1 cut(s) | 205 |
| BsaHI | GRCGYC | 1 cut(s) | 135 |
| BsaWI | WCCGGW | 1 cut(s) | 58 |
| BseRI | GAGGAG | 2 cut(s) | 45, 224 |
| BshFI | GGCC | 1 cut(s) | 23 |
| BsiSI | CCGG | 2 cut(s) | 20, 59 |
| BsmI | GAATGC | 1 cut(s) | 250 |
| BsnI | GGCC | 1 cut(s) | 23 |
| Bsp119I | TTCGAA | 1 cut(s) | 83 |
| Bsp1407I | TGTACA | 1 cut(s) | 63 |
| Bsp143I | GATC | 2 cut(s) | 172, 265 |
| BspANI | GGCC | 1 cut(s) | 23 |
| BspHI | TCATGA | 1 cut(s) | 175 |
| BspPI | GGATC | 1 cut(s) | 260 |
| BspT104I | TTCGAA | 1 cut(s) | 83 |
| BsrGI | TGTACA | 1 cut(s) | 63 |
| BssMI | GATC | 2 cut(s) | 172, 265 |
| BssNI | GRCGYC | 1 cut(s) | 135 |
| BstACI | GRCGYC | 1 cut(s) | 135 |
| BstAUI | TGTACA | 1 cut(s) | 63 |
| BstBAI | YACGTR | 1 cut(s) | 205 |
| BstBI | TTCGAA | 1 cut(s) | 83 |
| BstC8I | GCNNGC | 1 cut(s) | 76 |
| BstDEI | CTNAG | 1 cut(s) | 167 |
| BstKTI | GATC | 2 cut(s) | 175, 268 |
| BstMBI | GATC | 2 cut(s) | 172, 265 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 196 |
| BstNSI | RCATGY | 1 cut(s) | 34 |
| BsuRI | GGCC | 1 cut(s) | 23 |
| Cac8I | GCNNGC | 1 cut(s) | 76 |
| CciI | TCATGA | 1 cut(s) | 175 |
| Csp6I | GTAC | 1 cut(s) | 64 |
| CviAII | CATG | 3 cut(s) | 31, 70, 176 |
| CviJI | RGCY | 5 cut(s) | 23, 49, 108, 152, 190 |
| CviKI_1 | RGCY | 5 cut(s) | 23, 49, 108, 152, 190 |
| CviQI | GTAC | 1 cut(s) | 64 |
| DdeI | CTNAG | 1 cut(s) | 167 |
| DpnI | GATC | 2 cut(s) | 174, 267 |
| DpnII | GATC | 2 cut(s) | 172, 265 |
| EaeI | YGGCCR | 1 cut(s) | 21 |
| FaeI | CATG | 3 cut(s) | 34, 73, 179 |
| FaiI | YATR | 8 cut(s) | 32, 71, 111, 177, 216, 222, 224, 236 |
| FatI | CATG | 3 cut(s) | 30, 69, 175 |
| FauNDI | CATATG | 1 cut(s) | 222 |
| FbaI | TGATCA | 1 cut(s) | 172 |
| FblI | GTMKAC | 1 cut(s) | 261 |
| HaeIII | GGCC | 1 cut(s) | 23 |
| HapII | CCGG | 2 cut(s) | 20, 59 |
| Hin1I | GRCGYC | 1 cut(s) | 135 |
| Hin1II | CATG | 3 cut(s) | 34, 73, 179 |
| HindIII | AAGCTT | 1 cut(s) | 188 |
| HpaII | CCGG | 2 cut(s) | 20, 59 |
| Hpy166II | GTNNAC | 2 cut(s) | 64, 262 |
| Hpy188I | TCNGA | 1 cut(s) | 133 |
| Hpy188III | TCNNGA | 1 cut(s) | 176 |
| Hpy8I | GTNNAC | 2 cut(s) | 64, 262 |
| HpyAV | CCTTC | 1 cut(s) | 142 |
| HpyCH4IV | ACGT | 3 cut(s) | 90, 135, 204 |
| HpyCH4V | TGCA | 2 cut(s) | 34, 125 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 196 |
| HpyF3I | CTNAG | 1 cut(s) | 167 |
| HpySE526I | ACGT | 3 cut(s) | 90, 135, 204 |
| Hsp92I | GRCGYC | 1 cut(s) | 135 |
| Hsp92II | CATG | 3 cut(s) | 34, 73, 179 |
| Ksp22I | TGATCA | 1 cut(s) | 172 |
| Kzo9I | GATC | 2 cut(s) | 172, 265 |
| LmnI | GCTCC | 1 cut(s) | 196 |
| LpnPI | CCDG | 2 cut(s) | 33, 72 |
| LweI | GCATC | 1 cut(s) | 112 |
| MaeII | ACGT | 3 cut(s) | 90, 135, 204 |
| MalI | GATC | 2 cut(s) | 174, 267 |
| MboI | GATC | 2 cut(s) | 172, 265 |
| MboII | GAAGA | 1 cut(s) | 274 |
| MluCI | AATT | 1 cut(s) | 13 |
| MnlI | CCTC | 5 cut(s) | 66, 105, 107, 202, 279 |
| MseI | TTAA | 2 cut(s) | 27, 159 |
| MslI | CAYNNNNRTG | 1 cut(s) | 29 |
| MspI | CCGG | 2 cut(s) | 20, 59 |
| Mva1269I | GAATGC | 1 cut(s) | 250 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 196 |
| NdeI | CATATG | 1 cut(s) | 222 |
| NdeII | GATC | 2 cut(s) | 172, 265 |
| NlaIII | CATG | 3 cut(s) | 34, 73, 179 |
| NspI | RCATGY | 1 cut(s) | 34 |
| NspV | TTCGAA | 1 cut(s) | 83 |
| PagI | TCATGA | 1 cut(s) | 175 |
| PctI | GAATGC | 1 cut(s) | 250 |
| Ppu21I | YACGTR | 1 cut(s) | 205 |
| RsaI | GTAC | 1 cut(s) | 65 |
| RsaNI | GTAC | 1 cut(s) | 64 |
| RseI | CAYNNNNRTG | 1 cut(s) | 29 |
| SaqAI | TTAA | 2 cut(s) | 27, 159 |
| Sau3AI | GATC | 2 cut(s) | 172, 265 |
| SetI | ASST | 6 cut(s) | 51, 93, 138, 168, 192, 207 |
| SfaNI | GCATC | 1 cut(s) | 112 |
| SfuI | TTCGAA | 1 cut(s) | 83 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 29 |
| Sse9I | AATT | 1 cut(s) | 13 |
| TaiI | ACGT | 3 cut(s) | 93, 138, 207 |
| TaqI | TCGA | 1 cut(s) | 83 |
| TasI | AATT | 1 cut(s) | 13 |
| TatI | WGTACW | 1 cut(s) | 63 |
| Tru1I | TTAA | 2 cut(s) | 27, 159 |
| Tru9I | TTAA | 2 cut(s) | 27, 159 |
| TspDTI | ATGAA | 1 cut(s) | 220 |
| XceI | RCATGY | 1 cut(s) | 34 |
| XmiI | GTMKAC | 1 cut(s) | 261 |
| ZraI | GACGTC | 1 cut(s) | 136 |