FvH4_6g41950
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000706
Physical Location & Seq
Reverse (-)
32843669 .. 32846752
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41950.t1

Sequence Viewer

Length: 1191 bp
ATGGCCAAGTACGCTTCAAACCTTCTGCTTCTTGTGGCTCTACTTTGTGCTTTTGCAGCTGCAAGAATCAACTGTCTTGATCAGAATGGTATCTGCAAATCACTGGTGGAAACACAAGGCTATACTTGCGAGGATCATCAAGTAACTACAGAAGATGGTTACATCCTTGGTTTGCAGAGAATCCCATCTGGAAAGTCCAGTAACAATTCGACGGCTCAGAAGCTGCCAGTTTTATTGCAACATGGGCTTATGGGGGATGCTGGATCATGGCTGCTCAATCCGCCAGATCAAGCTTTGGCATTTATCTTGGCCGATAAAGGGTTTGATGTATGGCTTGCTAATGCTCGAGGAACGCAGGCTAGTCGCGGACATAAGTCACTCAATAATATCGATCCGGCTTACTGGGAATGGACATGGGATCAATTGGCTGCTTATGATCTTCCTGCCTTCTTCAATTATGTGAATAATCAAACAGGACAGAAAGTACACTATGTTGGCCATTCATTGGGAACATTGTCAGTGCTAGCTGCTTTGTCACAACAAAAGCTGAGTACCACACTAAGATCAGCAGCTTTGCTTTGTCCAGTTGCTTATCTGGGTCAGATGTCCACATTGTTGGGAAGAATTTCTGCTCAATCATATACTGCCGAAGGACTCTACGCCACTGGAGTCCGGGAATATCCTCCTCCAGGGGAAGCTGCAGCACAAAACTTGGAGACTTTTCTCTGCTCACAACCGGGTGTGGACTGCTCCAATTTTATGGCCGCCGTAACCGGTCCTAATTGCTGCATAACTCCTACGAGTTCAATTCTTCTCTTTCAGCATATACCACAGTCCACTTCCACAAAAAACATGGTACATATGTCTCAAATGATCAGGAGAGGAACAATAACAATGTTCGATTACGTTTTACCGGCCATTAACATGCAACGCTACAATCAGCTTACTCCTCCAGTCTACAACATGGCAAGCATTCCGGAAGATGTTCCTCTTTTCTTCGGCTATGGAGGGAGAGATGAACTTTCGGATGTCAACGATGTGAAGGCTTTGCTTAATGACCTGAGAGATCATGACAAAGACAAACTTGTGGAACAATACATAGAGGAGTATGCTCATATGGATTTCATTATGGCTGGGAATGCCAATCAAAAAGTCTATGATCCTCTGCTCACTTTTTTCGGGCAAAATTGA

Protein Analysis

397

Amino Acids

43.57

Weight (kDa)

5.33

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 34 - 93 1.3e-21 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 76 - 202 3.4e-11 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 77 - 215 5.7e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000706)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03230 FvH4_5g03471 FvH4_5g03472 FvH4_6g41950 FvH4_6g41951
malus_domestica MD09G1115700.v1.1 MD09G1115900.v1.1 MD17G1107600.v1.1 MD17G1107700.v1.1
prunus_persica Prupe.3G208700_v2.0.a1 Prupe.3G208800_v2.0.a1 Prupe.5G037500_v2.0.a1
pyrus_communis pycom09g04010 pycom09g04030 pycom17g10020 pycom17g10030
rosa_chinensis RchiOBHm_Chr2g0101861 RchiOBHm_Chr2g0157451 RchiOBHm_Chr2g0157461 RchiOBHm_Chr2g0157841 RchiOBHm_Chr2g0157851 RchiOBHm_Chr3g0484721 RchiOBHm_Chr3g0484731 RchiOBHm_Chr3g0484741 RchiOBHm_Chr3g0484751 RchiOBHm_Chr6g0253931 RchiOBHm_Chr6g0253991
rosa_laevigata RLG00000014950 RLG00000014954 RLG00000020989 RLG00000021024 RLG00000021026 RLG00000021027 RLG00000021029 RLG00000021030
rosa_multiflora Rmu_sc0000195.1_g000023 Rmu_sc0000195.1_g000028 Rmu_sc0001689.1_g000007 Rmu_sc0001716.1_g000018 Rmu_sc0002441.1_g000012 Rmu_sc0004191.1_g000065 Rmu_sc0004473.1_g000004
rosa_roxburghii Rroxscaffold_1G00036760 Rroxscaffold_1G00036780 Rroxscaffold_2G00091900 Rroxscaffold_2G00092180 Rroxscaffold_2G00092200 Rroxscaffold_2G00127230 Rroxscaffold_7G00211340 Rroxscaffold_7G00211380
rosa_rugosa Rorug02G0102500 Rorug02G0206400 Rorug02G0206500 Rorug02G0461400 Rorug02G0464100 Rorug05G0548600 Rorug05G0548800
rosa_samantha Rh2BG155500 Rh2BG540100 Rh2BG540200 Rh2BG543500 Rh2BG543700 Rh2BG543800 Rh3CG296800 Rh6AG064700 Rh6AG065300
rosa_wichuraiana Rw2G020570 Rw2G043520 Rw2G043530 Rw2G043770 Rw2G043780 Rw3G023530 Rw3G023540 Rw6G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 505
AccI GTMKAC 1 cut(s) 957
AccII CGCG 1 cut(s) 366
AccIII TCCGGA 1 cut(s) 976
AciI CCGC 3 cut(s) 281, 366, 765
AclWI GGATC 5 cut(s) 141, 271, 386, 426, 1154
AcoI YGGCCR 5 cut(s) 3, 309, 496, 762, 915
AcsI RAATTY 1 cut(s) 624
AfaI GTAC 4 cut(s) 11, 486, 553, 858
AfiI CCNNNNNNNGG 3 cut(s) 318, 505, 689
AgeI ACCGGT 1 cut(s) 773
AgsI TTSAA 3 cut(s) 18, 454, 807
AjnI CCWGG 1 cut(s) 688
AluBI AGCT 8 cut(s) 59, 223, 293, 527, 547, 572, 698, 943
AluI AGCT 8 cut(s) 59, 223, 293, 527, 547, 572, 698, 943
Alw26I GTCTC 2 cut(s) 710, 870
AlwI GGATC 5 cut(s) 141, 271, 386, 426, 1154
AlwNI CAGNNNCTG 1 cut(s) 223
Ama87I CYCGRG 1 cut(s) 345
Aor13HI TCCGGA 1 cut(s) 976
AoxI GGCC 5 cut(s) 3, 309, 496, 762, 915
ApoI RAATTY 1 cut(s) 624
AsiGI ACCGGT 1 cut(s) 773
Asp700I GAANNNNTTC 2 cut(s) 625, 984
AspS9I GGNCC 1 cut(s) 776
AsuC2I CCSGG 2 cut(s) 674, 738
AsuNHI GCTAGC 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 345
AvaII GGWCC 1 cut(s) 776
BaeI ACNNNNGTAYC 2 cut(s) 848, 881
BalI TGGCCA 2 cut(s) 5, 498
BccI CCATC 2 cut(s) 149, 193
BceAI ACGGC 2 cut(s) 228, 752
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 1 cut(s) 690
BclI TGATCA 2 cut(s) 79, 873
BcnI CCSGG 2 cut(s) 674, 738
BcoDI GTCTC 2 cut(s) 710, 870
BfaI CTAG 2 cut(s) 360, 524
BfmI CTRYAG 2 cut(s) 147, 699
Bme1390I CCNGG 3 cut(s) 674, 690, 738
Bme18I GGWCC 1 cut(s) 776
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 776
BmrFI CCNGG 3 cut(s) 674, 690, 738
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 1 cut(s) 247
BmtI GCTAGC 1 cut(s) 527
BmuI ACTGGG 1 cut(s) 412
BoxI GACNNNNGTC 1 cut(s) 373
BpmI CTGGAG 3 cut(s) 672, 687, 936
BpuMI CCSGG 2 cut(s) 674, 738
Bsa29I ATCGAT 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 166, 689
BsaWI WCCGGW 2 cut(s) 773, 976
Bsc4I CCNNNNNNNGG 3 cut(s) 318, 505, 689
Bse118I RCCGGY 2 cut(s) 773, 913
Bse1I ACTGG 7 cut(s) 108, 198, 227, 407, 584, 670, 953
BseAI TCCGGA 1 cut(s) 976
BseBI CCWGG 1 cut(s) 690
BseCI ATCGAT 1 cut(s) 390
BseDI CCNNGG 2 cut(s) 166, 689
BseGI GGATG 3 cut(s) 162, 262, 1033
BseLI CCNNNNNNNGG 3 cut(s) 318, 505, 689
BseMII CTCAG 3 cut(s) 230, 539, 1052
BseNI ACTGG 7 cut(s) 108, 198, 227, 407, 584, 670, 953
BseRI GAGGAG 3 cut(s) 675, 939, 1118
BseYI CCCAGC 1 cut(s) 1133
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 5 cut(s) 5, 311, 498, 764, 917
BshTI ACCGGT 1 cut(s) 773
BshVI ATCGAT 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 6 cut(s) 395, 673, 737, 774, 914, 977
BslI CCNNNNNNNGG 3 cut(s) 318, 505, 689
BsmAI GTCTC 2 cut(s) 710, 870
BsmI GAATGC 2 cut(s) 972, 1144
BsnI GGCC 5 cut(s) 5, 311, 498, 764, 917
BsoBI CYCGRG 1 cut(s) 345
Bsp13I TCCGGA 1 cut(s) 976
BspACI CCGC 3 cut(s) 281, 366, 765
BspANI GGCC 5 cut(s) 5, 311, 498, 764, 917
BspCNI CTCAG 3 cut(s) 229, 540, 1053
BspDI ATCGAT 1 cut(s) 390
BspEI TCCGGA 1 cut(s) 976
BspFNI CGCG 1 cut(s) 366
BspHI TCATGA 1 cut(s) 1069
BspMAI CTGCAG 1 cut(s) 703
BspOI GCTAGC 1 cut(s) 527
BspPI GGATC 5 cut(s) 141, 271, 386, 426, 1154
BsrFI RCCGGY 2 cut(s) 773, 913
BsrI ACTGG 7 cut(s) 108, 198, 227, 407, 584, 670, 953
BssAI RCCGGY 2 cut(s) 773, 913
BssECI CCNNGG 2 cut(s) 166, 689
BssT1I CCWWGG 1 cut(s) 166
Bst2UI CCWGG 1 cut(s) 690
Bst4CI ACNGT 2 cut(s) 74, 834
BstC8I GCNNGC 4 cut(s) 336, 357, 525, 970
BstDEI CTNAG 4 cut(s) 216, 548, 560, 1061
BstENI CCTNNNNNAGG 1 cut(s) 687
BstF5I GGATG 3 cut(s) 162, 262, 1033
BstFNI CGCG 1 cut(s) 366
BstMAI GTCTC 2 cut(s) 710, 870
BstMWI GCNNNNNNNGC 6 cut(s) 11, 56, 126, 244, 280, 1139
BstNI CCWGG 1 cut(s) 690
BstNSI RCATGY 1 cut(s) 928
BstPAI GACNNNNGTC 1 cut(s) 373
BstSCI CCNGG 3 cut(s) 672, 688, 736
BstSFI CTRYAG 2 cut(s) 147, 699
BstUI CGCG 1 cut(s) 366
BstXI CCANNNNNNTGG 2 cut(s) 616, 760
Bsu15I ATCGAT 1 cut(s) 390
BsuRI GGCC 5 cut(s) 5, 311, 498, 764, 917
BsuTUI ATCGAT 1 cut(s) 390
BtsCI GGATG 3 cut(s) 162, 262, 1033
BtsIMutI CAGTG 3 cut(s) 101, 525, 663
Cac8I GCNNGC 4 cut(s) 336, 357, 525, 970
CaiI CAGNNNCTG 1 cut(s) 223
CciI TCATGA 1 cut(s) 1069
Cfr10I RCCGGY 2 cut(s) 773, 913
Cfr13I GGNCC 1 cut(s) 776
ClaI ATCGAT 1 cut(s) 390
Csp6I GTAC 4 cut(s) 10, 485, 552, 857
CspAI ACCGGT 1 cut(s) 773
CviAII CATG 7 cut(s) 242, 267, 414, 853, 925, 964, 1070
CviQI GTAC 4 cut(s) 10, 485, 552, 857
DdeI CTNAG 4 cut(s) 216, 548, 560, 1061
EaeI YGGCCR 5 cut(s) 3, 309, 496, 762, 915
EciI GGCGGA 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 776
Eco88I CYCGRG 1 cut(s) 345
EcoNI CCTNNNNNAGG 1 cut(s) 687
EcoRII CCWGG 1 cut(s) 688
EcoT14I CCWWGG 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 166
FaeI CATG 7 cut(s) 245, 270, 417, 856, 928, 967, 1073
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FatI CATG 7 cut(s) 241, 266, 413, 852, 924, 963, 1069
FauNDI CATATG 2 cut(s) 861, 1116
FbaI TGATCA 2 cut(s) 79, 873
FblI GTMKAC 1 cut(s) 957
FokI GGATG 3 cut(s) 149, 269, 1040
FspBI CTAG 2 cut(s) 360, 524
GsaI CCCAGC 1 cut(s) 1137
GsuI CTGGAG 3 cut(s) 672, 687, 936
HaeIII GGCC 5 cut(s) 5, 311, 498, 764, 917
HapII CCGG 6 cut(s) 395, 673, 737, 774, 914, 977
Hin1II CATG 7 cut(s) 245, 270, 417, 856, 928, 967, 1073
HincII GTYRAC 1 cut(s) 1033
HindII GTYRAC 1 cut(s) 1033
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 4 cut(s) 66, 180, 654, 669
HpaII CCGG 6 cut(s) 395, 673, 737, 774, 914, 977
Hpy166II GTNNAC 6 cut(s) 487, 609, 745, 837, 958, 1033
Hpy188I TCNGA 4 cut(s) 84, 219, 603, 1027
Hpy188III TCNNGA 5 cut(s) 77, 189, 877, 977, 1070
Hpy8I GTNNAC 6 cut(s) 487, 609, 745, 837, 958, 1033
Hpy99I CGWCG 1 cut(s) 214
HpyAV CCTTC 4 cut(s) 32, 457, 644, 1036
HpyCH4III ACNGT 2 cut(s) 74, 834
HpyCH4IV ACGT 1 cut(s) 906
HpyCH4V TGCA 8 cut(s) 56, 62, 96, 175, 238, 701, 789, 928
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 56, 126, 244, 280, 1139
HpyF3I CTNAG 4 cut(s) 216, 548, 560, 1061
HpySE526I ACGT 1 cut(s) 906
Hsp92II CATG 7 cut(s) 245, 270, 417, 856, 928, 967, 1073
Kpn2I TCCGGA 1 cut(s) 976
Ksp22I TGATCA 2 cut(s) 79, 873
LmnI GCTCC 1 cut(s) 755
LweI GCATC 1 cut(s) 247
MaeI CTAG 2 cut(s) 360, 524
MaeII ACGT 1 cut(s) 906
MaeIII GTNAC 6 cut(s) 142, 158, 200, 375, 534, 769
MboII GAAGA 7 cut(s) 164, 431, 442, 633, 803, 988, 992
MfeI CAATTG 1 cut(s) 422
MlsI TGGCCA 2 cut(s) 5, 498
MluCI AATT 8 cut(s) 205, 422, 454, 624, 754, 781, 807, 1186
MluNI TGGCCA 2 cut(s) 5, 498
MlyI GAGTC 2 cut(s) 648, 678
Mox20I TGGCCA 2 cut(s) 5, 498
MroI TCCGGA 1 cut(s) 976
MroXI GAANNNNTTC 2 cut(s) 625, 984
MscI TGGCCA 2 cut(s) 5, 498
MseI TTAA 2 cut(s) 921, 1053
MslI CAYNNNNRTG 1 cut(s) 923
Msp20I TGGCCA 2 cut(s) 5, 498
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 6 cut(s) 395, 673, 737, 774, 914, 977
MspR9I CCNGG 3 cut(s) 674, 690, 738
MunI CAATTG 1 cut(s) 422
Mva1269I GAATGC 2 cut(s) 972, 1144
MvaI CCWGG 1 cut(s) 690
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 6 cut(s) 11, 56, 126, 244, 280, 1139
NciI CCSGG 2 cut(s) 674, 738
NdeI CATATG 2 cut(s) 861, 1116
NheI GCTAGC 1 cut(s) 523
NlaIII CATG 7 cut(s) 245, 270, 417, 856, 928, 967, 1073
NmuCI GTSAC 2 cut(s) 375, 534
NspI RCATGY 1 cut(s) 928
PaeR7I CTCGAG 1 cut(s) 345
PagI TCATGA 1 cut(s) 1069
PctI GAATGC 2 cut(s) 972, 1144
PdmI GAANNNNTTC 2 cut(s) 625, 984
PfeI GAWTC 2 cut(s) 66, 180
PflMI CCANNNNNTGG 1 cut(s) 505
PfoI TCCNGGA 1 cut(s) 672
PinAI ACCGGT 1 cut(s) 773
PleI GAGTC 2 cut(s) 648, 677
PpsI GAGTC 2 cut(s) 648, 677
PshAI GACNNNNGTC 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 688
PspFI CCCAGC 1 cut(s) 1133
PspGI CCWGG 1 cut(s) 688
PspPI GGNCC 1 cut(s) 776
PspXI VCTCGAGB 1 cut(s) 345
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 223
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 4 cut(s) 11, 486, 553, 858
RsaNI GTAC 4 cut(s) 10, 485, 552, 857
RseI CAYNNNNRTG 1 cut(s) 923
SaqAI TTAA 2 cut(s) 921, 1053
Sau96I GGNCC 1 cut(s) 776
SchI GAGTC 2 cut(s) 648, 678
ScrFI CCNGG 3 cut(s) 674, 690, 738
SfaNI GCATC 1 cut(s) 247
SfcI CTRYAG 2 cut(s) 147, 699
Sfr274I CTCGAG 1 cut(s) 345
SinI GGWCC 1 cut(s) 776
SlaI CTCGAG 1 cut(s) 345
SmiMI CAYNNNNRTG 1 cut(s) 923
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse9I AATT 8 cut(s) 205, 422, 454, 624, 754, 781, 807, 1186
SsiI CCGC 3 cut(s) 281, 366, 765
SspMI CTAG 2 cut(s) 360, 524
StyD4I CCNGG 3 cut(s) 672, 688, 736
StyI CCWWGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 74, 834
TaiI ACGT 1 cut(s) 909
TaqI TCGA 4 cut(s) 209, 346, 390, 900
TasI AATT 8 cut(s) 205, 422, 454, 624, 754, 781, 807, 1186
TatI WGTACW 1 cut(s) 484
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 2 cut(s) 66, 180
Tru1I TTAA 2 cut(s) 921, 1053
Tru9I TTAA 2 cut(s) 921, 1053
TscAI CASTG 3 cut(s) 108, 525, 670
TseFI GTSAC 2 cut(s) 375, 534
Tsp45I GTSAC 2 cut(s) 375, 534
TspDTI ATGAA 3 cut(s) 492, 1032, 1114
TspRI CASTG 3 cut(s) 108, 525, 670
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 776
XagI CCTNNNNNAGG 1 cut(s) 687
XapI RAATTY 1 cut(s) 624
XceI RCATGY 1 cut(s) 928
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XhoI CTCGAG 1 cut(s) 345
XmiI GTMKAC 1 cut(s) 957
XmnI GAANNNNTTC 2 cut(s) 625, 984
XspI CTAG 2 cut(s) 360, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.