Rw2G043530
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000706
Physical Location & Seq
Forward (+)
69978867 .. 69980869
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043530.1

Sequence Viewer

Length: 1242 bp
ATGGTAGCTATTTTGAGGCGGCGCGGATCGGTGGACAAGATGCTCTTTCGATTCTTTGTAAACGGTGAGCTTCTCTGTTGGAGGAGTTGTGCCGGCATAATGGTGGCCAGGCTGGCTCACGGGTTTTGGTGCTTCGTCGGCAGCGGCGGCTGGTGGTTCTTCTCCATGGTGGCTGGGCTTTGCGGCGGGGAGCTTTTTTGTGTTTCAGGAGTTGCACAAACCAGAGGTCATGATATTCACAATGGTATTTGCAAATCACTTGTGGAGACACGAGGCTATATTTGCCAAGAACATCAAGTAACAACAGAAGATGGTTACATTCTAGGGTTGCAGAGAATTCCATATGGACGGTCTGGTAGAAATTCAGCCACAGATCAGAAGCCGCCTGTTTTGATGCAACATGGGCTTATAATTGATGCTTCATCATGGCTACTCAATCCACCGGATCAGGCTTTGGCGTTCATCTTGGCTGACAAAGGGTTTGATGTATGGCTTGCTAATTCTCGAGGAACGGACTCTAGCCGTGGACACAAATCACTTACTACTAATGATCCTGCTTATTGGGAATGGACATGGGAACAGTTGGCTGCTTATGATCTTCCTGCTTTGTTCAATTATGTGAACAAGCAAACAGGACAGAAGTCACACTACGTTGGCCATTCCTTAGGAACATTGACAATACTACCTGCCTTGTCCCAACAAAAATTAAGAAACTCATTGAGATCAGCTGCTTTGCTTAGCCCAATTGCTTATCTGGGTCAGATATCCACACTGTTTATAAGAGTTACTACTCAAACATTTTATGCTGAGCAATTCTACATCGTTGGTCCAAATTGTTGCCTAAATCCTTCAAGTTTAGATCTTTACTATAATCATACACCACAATCCACTGCCACAAAGAATGCCATACATTTGTCTCAGATGATCAGAAGTGGAACCATAAGAATGTTCGATTACACTCTTCCACTCACAAATCTGCAGCACTACAATATGTTTACTCCTCCAGTGTACAACATGGCAAACATTCAGAAAGACATTCCTCTTTTCCTCAGCTATGGAGGGAGAGATGCACTTTCAGACGTCAACGATGTGAAGACTTTGCTTAATGACCTCAATGATCATGACAAGGACAAGCTTGTCGAACAATACATAGAGGAGTATGCTCATTATGATTTCGTTATGGGTGAGAATGCCCGTCAGAAAGTCTATGATCCTCTGCTCGCTTTCTTCAGGCAACATTGA

Protein Analysis

413

Amino Acids

46.7

Weight (kDa)

7.21

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 86 - 146 9.2e-20 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 128 - 251 1.5e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000706)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03230 FvH4_5g03471 FvH4_5g03472 FvH4_6g41950 FvH4_6g41951
malus_domestica MD09G1115700.v1.1 MD09G1115900.v1.1 MD17G1107600.v1.1 MD17G1107700.v1.1
prunus_persica Prupe.3G208700_v2.0.a1 Prupe.3G208800_v2.0.a1 Prupe.5G037500_v2.0.a1
pyrus_communis pycom09g04010 pycom09g04030 pycom17g10020 pycom17g10030
rosa_chinensis RchiOBHm_Chr2g0101861 RchiOBHm_Chr2g0157451 RchiOBHm_Chr2g0157461 RchiOBHm_Chr2g0157841 RchiOBHm_Chr2g0157851 RchiOBHm_Chr3g0484721 RchiOBHm_Chr3g0484731 RchiOBHm_Chr3g0484741 RchiOBHm_Chr3g0484751 RchiOBHm_Chr6g0253931 RchiOBHm_Chr6g0253991
rosa_laevigata RLG00000014950 RLG00000014954 RLG00000020989 RLG00000021024 RLG00000021026 RLG00000021027 RLG00000021029 RLG00000021030
rosa_multiflora Rmu_sc0000195.1_g000023 Rmu_sc0000195.1_g000028 Rmu_sc0001689.1_g000007 Rmu_sc0001716.1_g000018 Rmu_sc0002441.1_g000012 Rmu_sc0004191.1_g000065 Rmu_sc0004473.1_g000004
rosa_roxburghii Rroxscaffold_1G00036760 Rroxscaffold_1G00036780 Rroxscaffold_2G00091900 Rroxscaffold_2G00092180 Rroxscaffold_2G00092200 Rroxscaffold_2G00127230 Rroxscaffold_7G00211340 Rroxscaffold_7G00211380
rosa_rugosa Rorug02G0102500 Rorug02G0206400 Rorug02G0206500 Rorug02G0461400 Rorug02G0464100 Rorug05G0548600 Rorug05G0548800
rosa_samantha Rh2BG155500 Rh2BG540100 Rh2BG540200 Rh2BG543500 Rh2BG543700 Rh2BG543800 Rh3CG296800 Rh6AG064700 Rh6AG065300
rosa_wichuraiana Rw2G020570 Rw2G043520 Rw2G043530 Rw2G043770 Rw2G043780 Rw3G023530 Rw3G023540 Rw6G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 410, 779
AasI GACNNNNNNGTC 1 cut(s) 1136
AatII GACGTC 1 cut(s) 1083
Acc36I ACCTGC 1 cut(s) 694
AccII CGCG 1 cut(s) 24
AciI CCGC 7 cut(s) 19, 24, 144, 147, 183, 186, 383
AclWI GGATC 4 cut(s) 34, 453, 545, 1205
AcoI YGGCCR 2 cut(s) 105, 655
AcsI RAATTY 2 cut(s) 336, 361
AcuI CTGAAG 1 cut(s) 1213
AcyI GRCGYC 1 cut(s) 1080
AfaI GTAC 1 cut(s) 1010
AgsI TTSAA 2 cut(s) 613, 852
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 6 cut(s) 8, 70, 193, 728, 1053, 1135
AluI AGCT 6 cut(s) 8, 70, 193, 728, 1053, 1135
Alw26I GTCTC 2 cut(s) 260, 921
AlwI GGATC 4 cut(s) 34, 453, 545, 1205
Ama87I CYCGRG 1 cut(s) 504
AoxI GGCC 2 cut(s) 105, 655
ApeKI GCWGC 4 cut(s) 141, 587, 728, 979
ApoI RAATTY 2 cut(s) 336, 361
ArsI GACNNNNNNTTYG 4 cut(s) 211, 243, 464, 496
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 827
AsuHPI GGTGA 2 cut(s) 77, 1196
AvaI CYCGRG 1 cut(s) 504
AvaII GGWCC 1 cut(s) 827
AxyI CCTNAGG 1 cut(s) 664
BalI TGGCCA 2 cut(s) 107, 657
BarI GAAGNNNNNNTAC 2 cut(s) 632, 664
BauI CACGAG 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 1100
BbvCI CCTCAGC 1 cut(s) 1049
BbvI GCAGC 4 cut(s) 153, 574, 715, 991
BccI CCATC 1 cut(s) 305
BceAI ACGGC 1 cut(s) 507
BciT130I CCWGG 1 cut(s) 109
BclI TGATCA 2 cut(s) 924, 1117
BcoDI GTCTC 2 cut(s) 260, 921
BfaI CTAG 2 cut(s) 323, 519
BfmI CTRYAG 1 cut(s) 977
BfuAI ACCTGC 1 cut(s) 694
BglI GCCNNNNNGGC 1 cut(s) 113
BglII AGATCT 1 cut(s) 859
BisI GCNGC 9 cut(s) 20, 142, 145, 148, 184, 383, 588, 729, 980
BlpI GCTNAGC 2 cut(s) 737, 807
BlsI GCNGC 9 cut(s) 21, 143, 146, 149, 185, 384, 589, 730, 981
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 1 cut(s) 827
BmeT110I CYCGRG 1 cut(s) 504
BmgT120I GGNCC 1 cut(s) 827
BmiI GGNNCC 1 cut(s) 937
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 4 cut(s) 30, 384, 406, 1057
BoxI GACNNNNGTC 1 cut(s) 640
BpiI GAAGAC 1 cut(s) 1100
BpmI CTGGAG 1 cut(s) 987
Bpu10I CCTNAGC 1 cut(s) 1049
Bpu1102I GCTNAGC 2 cut(s) 737, 807
BsaHI GRCGYC 1 cut(s) 1080
BsaJI CCNNGG 2 cut(s) 165, 523
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 1004
Bse21I CCTNAGG 1 cut(s) 664
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 165, 523
BseMII CTCAG 3 cut(s) 798, 932, 1063
BseNI ACTGG 1 cut(s) 1004
BseRI GAGGAG 3 cut(s) 97, 990, 1169
BseXI GCAGC 4 cut(s) 153, 574, 715, 991
BseYI CCCAGC 1 cut(s) 173
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 107, 657
BsiHKCI CYCGRG 1 cut(s) 504
BsiSI CCGG 2 cut(s) 93, 443
BslFI GGGAC 1 cut(s) 679
BsmAI GTCTC 2 cut(s) 260, 921
BsmFI GGGAC 1 cut(s) 679
BsmI GAATGC 2 cut(s) 907, 1195
BsnI GGCC 2 cut(s) 107, 657
BsoBI CYCGRG 1 cut(s) 504
Bsp1407I TGTACA 1 cut(s) 1008
Bsp1720I GCTNAGC 2 cut(s) 737, 807
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 7 cut(s) 19, 24, 144, 147, 183, 186, 383
BspANI GGCC 2 cut(s) 107, 657
BspCNI CTCAG 3 cut(s) 799, 931, 1062
BspFNI CGCG 1 cut(s) 24
BspHI TCATGA 2 cut(s) 229, 1120
BspLI GGNNCC 1 cut(s) 937
BspMAI CTGCAG 1 cut(s) 981
BspMI ACCTGC 1 cut(s) 694
BspPI GGATC 4 cut(s) 34, 453, 545, 1205
BsrFI RCCGGY 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 1008
BsrI ACTGG 1 cut(s) 1004
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 165, 523
BssNI GRCGYC 1 cut(s) 1080
BssSI CACGAG 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 4 cut(s) 65, 351, 582, 774
Bst6I CTCTTC 1 cut(s) 966
BstACI GRCGYC 1 cut(s) 1080
BstAUI TGTACA 1 cut(s) 1008
BstC8I GCNNGC 4 cut(s) 94, 114, 495, 1221
BstDEI CTNAG 5 cut(s) 664, 737, 807, 918, 1049
BstDSI CCRYGG 2 cut(s) 165, 523
BstFNI CGCG 1 cut(s) 24
BstHHI GCGC 1 cut(s) 24
BstMAI GTCTC 2 cut(s) 260, 921
BstMWI GCNNNNNNNGC 5 cut(s) 113, 138, 147, 282, 403
BstNI CCWGG 1 cut(s) 109
BstPAI GACNNNNGTC 1 cut(s) 640
BstSCI CCNGG 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 977
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 4 cut(s) 153, 574, 715, 991
BstV2I GAAGAC 1 cut(s) 1100
BstX2I RGATCY 1 cut(s) 859
BstYI RGATCY 1 cut(s) 859
Bsu36I CCTNAGG 1 cut(s) 664
BsuRI GGCC 2 cut(s) 107, 657
BtgI CCRYGG 2 cut(s) 165, 523
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 3 cut(s) 770, 888, 1011
BveI ACCTGC 1 cut(s) 694
Cac8I GCNNGC 4 cut(s) 94, 114, 495, 1221
CciI TCATGA 2 cut(s) 229, 1120
CfoI GCGC 1 cut(s) 24
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 827
Csp6I GTAC 1 cut(s) 1009
CviAII CATG 7 cut(s) 166, 230, 401, 426, 573, 1015, 1121
CviQI GTAC 1 cut(s) 1009
DdeI CTNAG 5 cut(s) 664, 737, 807, 918, 1049
DrdI GACNNNNNNGTC 1 cut(s) 1136
DseDI GACNNNNNNGTC 1 cut(s) 1136
EaeI YGGCCR 2 cut(s) 105, 655
Eam1104I CTCTTC 1 cut(s) 966
EarI CTCTTC 1 cut(s) 966
Eco130I CCWWGG 1 cut(s) 165
Eco32I GATATC 1 cut(s) 765
Eco47I GGWCC 1 cut(s) 827
Eco57I CTGAAG 1 cut(s) 1213
Eco81I CCTNAGG 1 cut(s) 664
Eco88I CYCGRG 1 cut(s) 504
EcoRI GAATTC 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 107
EcoRV GATATC 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 7 cut(s) 169, 233, 404, 429, 576, 1018, 1124
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 1 cut(s) 679
FatI CATG 7 cut(s) 165, 229, 400, 425, 572, 1014, 1120
FauI CCCGC 1 cut(s) 179
FauNDI CATATG 1 cut(s) 343
FbaI TGATCA 2 cut(s) 924, 1117
Fnu4HI GCNGC 9 cut(s) 20, 142, 145, 148, 184, 383, 588, 729, 980
Fsp4HI GCNGC 9 cut(s) 20, 142, 145, 148, 184, 383, 588, 729, 980
FspBI CTAG 2 cut(s) 323, 519
GlaI GCGC 1 cut(s) 23
GluI GCNGC 9 cut(s) 20, 142, 145, 148, 184, 383, 588, 729, 980
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 2 cut(s) 107, 657
HapII CCGG 2 cut(s) 93, 443
HhaI GCGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 1080
Hin1II CATG 7 cut(s) 169, 233, 404, 429, 576, 1018, 1124
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HincII GTYRAC 1 cut(s) 1084
HindII GTYRAC 1 cut(s) 1084
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 2 cut(s) 51, 515
HpaII CCGG 2 cut(s) 93, 443
HphI GGTGA 2 cut(s) 77, 1196
Hpy166II GTNNAC 7 cut(s) 34, 61, 527, 622, 996, 1009, 1084
Hpy188I TCNGA 7 cut(s) 378, 762, 921, 929, 1029, 1078, 1200
Hpy188III TCNNGA 4 cut(s) 207, 230, 504, 1121
Hpy8I GTNNAC 7 cut(s) 34, 61, 527, 622, 996, 1009, 1084
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 1 cut(s) 858
HpyCH4III ACNGT 4 cut(s) 65, 351, 582, 774
HpyCH4IV ACGT 2 cut(s) 651, 1080
HpyCH4V TGCA 6 cut(s) 215, 252, 331, 397, 979, 1070
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 138, 147, 282, 403
HpyF3I CTNAG 5 cut(s) 664, 737, 807, 918, 1049
HpySE526I ACGT 2 cut(s) 651, 1080
Hsp92I GRCGYC 1 cut(s) 1080
Hsp92II CATG 7 cut(s) 169, 233, 404, 429, 576, 1018, 1124
HspAI GCGC 1 cut(s) 22
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Ksp22I TGATCA 2 cut(s) 924, 1117
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 4 cut(s) 153, 574, 715, 991
LweI GCATC 4 cut(s) 30, 384, 406, 1057
MaeI CTAG 2 cut(s) 323, 519
MaeII ACGT 2 cut(s) 651, 1080
MaeIII GTNAC 4 cut(s) 298, 314, 642, 784
MboII GAAGA 6 cut(s) 151, 320, 590, 953, 1105, 1219
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 1 cut(s) 859
MlsI TGGCCA 2 cut(s) 107, 657
MluCI AATT 9 cut(s) 336, 361, 411, 499, 613, 704, 744, 812, 832
MluNI TGGCCA 2 cut(s) 107, 657
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 1 cut(s) 59
Mox20I TGGCCA 2 cut(s) 107, 657
MroNI GCCGGC 1 cut(s) 92
MscI TGGCCA 2 cut(s) 107, 657
MseI TTAA 2 cut(s) 707, 1104
MslI CAYNNNNRTG 2 cut(s) 101, 944
Msp20I TGGCCA 2 cut(s) 107, 657
MspA1I CMGCKG 2 cut(s) 144, 728
MspI CCGG 2 cut(s) 93, 443
MspR9I CCNGG 1 cut(s) 109
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 2 cut(s) 907, 1195
MvaI CCWGG 1 cut(s) 109
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 5 cut(s) 113, 138, 147, 282, 403
NaeI GCCGGC 1 cut(s) 94
NcoI CCATGG 1 cut(s) 165
NdeI CATATG 1 cut(s) 343
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 7 cut(s) 169, 233, 404, 429, 576, 1018, 1124
NlaIV GGNNCC 1 cut(s) 937
NmuCI GTSAC 1 cut(s) 642
PaeR7I CTCGAG 1 cut(s) 504
PagI TCATGA 2 cut(s) 229, 1120
PctI GAATGC 2 cut(s) 907, 1195
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 9 cut(s) 21, 143, 146, 149, 185, 384, 589, 730, 981
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
PshAI GACNNNNGTC 1 cut(s) 640
PsiI TTATAA 2 cut(s) 410, 779
Psp6I CCWGG 1 cut(s) 107
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 937
PspPI GGNCC 1 cut(s) 827
PstI CTGCAG 1 cut(s) 981
PsuI RGATCY 1 cut(s) 859
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 1 cut(s) 1010
RsaNI GTAC 1 cut(s) 1009
RseI CAYNNNNRTG 2 cut(s) 101, 944
SaqAI TTAA 2 cut(s) 707, 1104
SatI GCNGC 9 cut(s) 20, 142, 145, 148, 184, 383, 588, 729, 980
Sau96I GGNCC 1 cut(s) 827
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 1 cut(s) 109
SfaNI GCATC 4 cut(s) 30, 384, 406, 1057
SfcI CTRYAG 1 cut(s) 977
Sfr274I CTCGAG 1 cut(s) 504
SinI GGWCC 1 cut(s) 827
SlaI CTCGAG 1 cut(s) 504
SmiMI CAYNNNNRTG 2 cut(s) 101, 944
SmlI CTYRAG 1 cut(s) 504
SmoI CTYRAG 1 cut(s) 504
Sse9I AATT 9 cut(s) 336, 361, 411, 499, 613, 704, 744, 812, 832
SsiI CCGC 7 cut(s) 19, 24, 144, 147, 183, 186, 383
SspMI CTAG 2 cut(s) 323, 519
StyD4I CCNGG 1 cut(s) 107
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 4 cut(s) 65, 351, 582, 774
TaiI ACGT 2 cut(s) 654, 1083
TaqI TCGA 4 cut(s) 49, 505, 951, 1140
TasI AATT 9 cut(s) 336, 361, 411, 499, 613, 704, 744, 812, 832
TatI WGTACW 1 cut(s) 1008
TauI GCSGC 5 cut(s) 22, 147, 150, 186, 385
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 2 cut(s) 707, 1104
Tru9I TTAA 2 cut(s) 707, 1104
TscAI CASTG 3 cut(s) 777, 895, 1011
TseFI GTSAC 1 cut(s) 642
TseI GCWGC 4 cut(s) 141, 587, 728, 979
Tsp45I GTSAC 1 cut(s) 642
TspDTI ATGAA 2 cut(s) 411, 451
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 3 cut(s) 777, 895, 1011
VpaK11BI GGWCC 1 cut(s) 827
XapI RAATTY 2 cut(s) 336, 361
XhoI CTCGAG 1 cut(s) 504
XspI CTAG 2 cut(s) 323, 519
ZraI GACGTC 1 cut(s) 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.