MD09G1115900.v1.1
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000706
Physical Location & Seq
Forward (+)
8884471 .. 8885187
717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1115900.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGGATTCACAATCTCAATAGTAACAATGTTAGGGTTCTTCATTCACACTGAATATGAATTAGTTTTTTGCAACCCATATACAGGCCCAAATTGCTGCCTCAGTTCTGCAAGCAAGGCTGCTTTATTTAAGAACTTCCCCCAGTCTACGGCGACAAAGAACATGATACATCTGTCTCAGATGGTCAGGATGGGAACCCTAGAAATGTACGATTATGGTAACGAAGACACGAACATGGAACACTACAAGCAGCCTACTCCTCCTGTGTACAACATGGCAAACATTCCGAAAGACGTTCCTCTTTTCCTCAGCTACGGGGGGAGAGATGCGCTTTCTGACGTTAATGACGTGGGGCTTCTGCTTGACAACCTCAAAGATCATGACAAGGACAAGCTTGTGGTAGAGTACATAGAGGATTATGCTCATATGGATTTTATTATGAGTGTGAATGCCAATCAACTTGTGTATGATCCTCTACTGGCCTTCTTCAAGCTCCATTAA

Protein Analysis

167

Amino Acids

18.9

Weight (kDa)

4.9

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000706)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03230 FvH4_5g03471 FvH4_5g03472 FvH4_6g41950 FvH4_6g41951
malus_domestica MD09G1115700.v1.1 MD09G1115900.v1.1 MD17G1107600.v1.1 MD17G1107700.v1.1
prunus_persica Prupe.3G208700_v2.0.a1 Prupe.3G208800_v2.0.a1 Prupe.5G037500_v2.0.a1
pyrus_communis pycom09g04010 pycom09g04030 pycom17g10020 pycom17g10030
rosa_chinensis RchiOBHm_Chr2g0101861 RchiOBHm_Chr2g0157451 RchiOBHm_Chr2g0157461 RchiOBHm_Chr2g0157841 RchiOBHm_Chr2g0157851 RchiOBHm_Chr3g0484721 RchiOBHm_Chr3g0484731 RchiOBHm_Chr3g0484741 RchiOBHm_Chr3g0484751 RchiOBHm_Chr6g0253931 RchiOBHm_Chr6g0253991
rosa_laevigata RLG00000014950 RLG00000014954 RLG00000020989 RLG00000021024 RLG00000021026 RLG00000021027 RLG00000021029 RLG00000021030
rosa_multiflora Rmu_sc0000195.1_g000023 Rmu_sc0000195.1_g000028 Rmu_sc0001689.1_g000007 Rmu_sc0001716.1_g000018 Rmu_sc0002441.1_g000012 Rmu_sc0004191.1_g000065 Rmu_sc0004473.1_g000004
rosa_roxburghii Rroxscaffold_1G00036760 Rroxscaffold_1G00036780 Rroxscaffold_2G00091900 Rroxscaffold_2G00092180 Rroxscaffold_2G00092200 Rroxscaffold_2G00127230 Rroxscaffold_7G00211340 Rroxscaffold_7G00211380
rosa_rugosa Rorug02G0102500 Rorug02G0206400 Rorug02G0206500 Rorug02G0461400 Rorug02G0464100 Rorug05G0548600 Rorug05G0548800
rosa_samantha Rh2BG155500 Rh2BG540100 Rh2BG540200 Rh2BG543500 Rh2BG543700 Rh2BG543800 Rh3CG296800 Rh6AG064700 Rh6AG065300
rosa_wichuraiana Rw2G020570 Rw2G043520 Rw2G043530 Rw2G043770 Rw2G043780 Rw3G023530 Rw3G023540 Rw6G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 146
AclWI GGATC 1 cut(s) 464
AfaI GTAC 3 cut(s) 209, 269, 407
AfiI CCNNNNNNNGG 2 cut(s) 83, 148
AgsI TTSAA 1 cut(s) 490
AjiI CACGTC 1 cut(s) 349
AluBI AGCT 3 cut(s) 312, 394, 493
AluI AGCT 3 cut(s) 312, 394, 493
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 1 cut(s) 464
AoxI GGCC 2 cut(s) 85, 480
ApeKI GCWGC 3 cut(s) 96, 119, 250
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 86
BaeI ACNNNNGTAYC 2 cut(s) 158, 191
BbsI GAAGAC 1 cut(s) 231
BbvCI CCTCAGC 1 cut(s) 308
BbvI GCAGC 3 cut(s) 83, 106, 262
BccI CCATC 2 cut(s) 175, 184
BceAI ACGGC 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 1 cut(s) 200
BisI GCNGC 3 cut(s) 97, 120, 251
BlsI GCNGC 3 cut(s) 98, 121, 252
BmgBI CACGTC 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 196
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 316
BmuI ACTGGG 1 cut(s) 136
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 308
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 148
Bse1I ACTGG 2 cut(s) 142, 483
BseGI GGATG 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 83, 148
BseMII CTCAG 3 cut(s) 115, 191, 322
BseNI ACTGG 2 cut(s) 142, 483
BseRI GAGGAG 1 cut(s) 249
BseXI GCAGC 3 cut(s) 83, 106, 262
BshFI GGCC 2 cut(s) 87, 482
BslI CCNNNNNNNGG 2 cut(s) 83, 148
BsmAI GTCTC 1 cut(s) 180
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 2 cut(s) 87, 482
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 2 cut(s) 376, 469
BspANI GGCC 2 cut(s) 87, 482
BspCNI CTCAG 3 cut(s) 114, 190, 321
BspHI TCATGA 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 196
BspPI GGATC 1 cut(s) 464
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 2 cut(s) 142, 483
BssMI GATC 2 cut(s) 376, 469
BstAUI TGTACA 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 3 cut(s) 101, 177, 308
BstF5I GGATG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 2 cut(s) 379, 472
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 2 cut(s) 376, 469
BstMWI GCNNNNNNNGC 2 cut(s) 93, 116
BstV1I GCAGC 3 cut(s) 83, 106, 262
BstV2I GAAGAC 1 cut(s) 231
BsuRI GGCC 2 cut(s) 87, 482
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 112
CciI TCATGA 1 cut(s) 379
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 86
Csp6I GTAC 3 cut(s) 208, 268, 406
CviAII CATG 4 cut(s) 163, 235, 274, 380
CviJI RGCY 8 cut(s) 87, 119, 253, 312, 355, 394, 482, 493
CviKI_1 RGCY 8 cut(s) 87, 119, 253, 312, 355, 394, 482, 493
CviQI GTAC 3 cut(s) 208, 268, 406
DdeI CTNAG 3 cut(s) 101, 177, 308
DpnI GATC 2 cut(s) 378, 471
DpnII GATC 2 cut(s) 376, 469
FaeI CATG 4 cut(s) 166, 238, 277, 383
FatI CATG 4 cut(s) 162, 234, 273, 379
FauNDI CATATG 1 cut(s) 426
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 3 cut(s) 97, 120, 251
FokI GGATG 1 cut(s) 202
Fsp4HI GCNGC 3 cut(s) 97, 120, 251
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 330
GluI GCNGC 3 cut(s) 97, 120, 251
HaeIII GGCC 2 cut(s) 87, 482
HhaI GCGC 1 cut(s) 331
Hin1II CATG 4 cut(s) 166, 238, 277, 383
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 1 cut(s) 6
Hpy166II GTNNAC 2 cut(s) 147, 268
Hpy188I TCNGA 3 cut(s) 180, 288, 337
Hpy188III TCNNGA 2 cut(s) 187, 380
Hpy8I GTNNAC 2 cut(s) 147, 268
HpyAV CCTTC 1 cut(s) 493
HpyCH4IV ACGT 3 cut(s) 294, 339, 348
HpyCH4V TGCA 2 cut(s) 72, 110
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 116
HpyF3I CTNAG 3 cut(s) 101, 177, 308
HpySE526I ACGT 3 cut(s) 294, 339, 348
Hsp92II CATG 4 cut(s) 166, 238, 277, 383
HspAI GCGC 1 cut(s) 329
Kzo9I GATC 2 cut(s) 376, 469
LmnI GCTCC 1 cut(s) 498
LpnPI CCDG 5 cut(s) 69, 155, 172, 276, 464
Lsp1109I GCAGC 3 cut(s) 83, 106, 262
LweI GCATC 1 cut(s) 316
MaeI CTAG 1 cut(s) 200
MaeII ACGT 3 cut(s) 294, 339, 348
MaeIII GTNAC 2 cut(s) 22, 218
MalI GATC 2 cut(s) 378, 471
MboI GATC 2 cut(s) 376, 469
MboII GAAGA 3 cut(s) 31, 236, 478
MluCI AATT 2 cut(s) 59, 91
MnlI CCTC 7 cut(s) 110, 270, 309, 317, 380, 406, 483
MseI TTAA 3 cut(s) 129, 342, 499
MslI CAYNNNNRTG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 454
MwoI GCNNNNNNNGC 2 cut(s) 93, 116
NdeI CATATG 1 cut(s) 426
NdeII GATC 2 cut(s) 376, 469
NlaIII CATG 4 cut(s) 166, 238, 277, 383
NlaIV GGNNCC 1 cut(s) 196
PagI TCATGA 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 6
PkrI GCNGC 3 cut(s) 98, 121, 252
PspN4I GGNNCC 1 cut(s) 196
PspPI GGNCC 1 cut(s) 86
RsaI GTAC 3 cut(s) 209, 269, 407
RsaNI GTAC 3 cut(s) 208, 268, 406
RseI CAYNNNNRTG 1 cut(s) 233
SaqAI TTAA 3 cut(s) 129, 342, 499
SatI GCNGC 3 cut(s) 97, 120, 251
Sau3AI GATC 2 cut(s) 376, 469
Sau96I GGNCC 1 cut(s) 86
SetI ASST 7 cut(s) 297, 314, 342, 351, 372, 396, 495
SfaNI GCATC 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 2 cut(s) 59, 91
SspMI CTAG 1 cut(s) 200
TaiI ACGT 3 cut(s) 297, 342, 351
TasI AATT 2 cut(s) 59, 91
TatI WGTACW 2 cut(s) 267, 405
TfiI GAWTC 1 cut(s) 6
Tru1I TTAA 3 cut(s) 129, 342, 499
Tru9I TTAA 3 cut(s) 129, 342, 499
TscAI CASTG 1 cut(s) 55
TseI GCWGC 3 cut(s) 96, 119, 250
TspDTI ATGAA 2 cut(s) 31, 72
TspRI CASTG 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 146
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.