FvH4_6g41951
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000706
Physical Location & Seq
Reverse (-)
32848299 .. 32851152
2854 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41951.t1

Sequence Viewer

Length: 1194 bp
ATGGCCAAGTACCCTTCAAAACCTCTGCTTCTGGCTCTACTTTGTGCTTTTGCAGCTGCAAGAATCAACAGTCTTGATCAAAATGGTATTTGCAAATCACTAGAGGAGACAGAAGGCTATACTTGCCAGGATCATCAAGTAACAACAGAAGATGGTTACATCCTCGGGTTGCAGAGGATTCCATCTGGAAAGTCAAATAACAATTCGACCGCTCAGAAGCTGCCTGTTTTATTGCAACATGGGCTTACGATGGATGGTGGAGCATGGCTGCTCAATCCGCCAGATCAAGCTTTGGCATTTATCTTGGCTGATAAAGGTTTTGATGTATGGCTTGCTAATGCTCGTGGAACGAAGGCTAGTCGCGGACATAATTCACTAAGTACTTATGATCCAGCTTACTGGGAATGGACATGGGATCAATTGGCTGTTTATGATCTTCCGGCTTTCTTCAACTATGTGAACAATCAAACTGGACAGAAAGTACACTATGTTGGCCATTCATTGATGTATAAGGGATCACTGAGGTATGAAGTATTTGAGTTACAGGGAACATTGTCAGTATTAGCTGCCTTGTCACAACAAAAGCTGAGTAACACACTAAGATCAGCTGCTTTGCTTAGCCCAGTTGCTTATCTAAATCAGATCACCACATTGTTGATACAAAGTTTCGCTCGAACATATGCTGCTAATGGGCTCTATGCCACTGGTCTCCGGGACTTCCCTCCCGTAGGGCAAGCTCTAGGAACGGTGTTGCAATTTCTCTGCTCAATGCCTGGTCCTAACTGCTGCCTTACTCCTACAAGCTCAAATCTTGTGTTTCAGCAGCATATACTACAGCCCACTTCCACAAAAAATATGGTGCATTTGTCTCAAATGATCAGGAGAGGAACAATAACGATGTTCGATTACATTTTCCCGGTCATTAACCTGCAACGCTACAATCAGCTTACTCCTCCGGTGTACAACATGGCAAGCATTCCGAAAGATGTTCCTCTTTTCTTCGGCTATGGAGGGAGAGATGCACTTTCGGATGTCAACGATGTGAAGGCTTTGCTTAATGACCTGAAAGATCATGACAAGGACAAACTTGTGGAACAATACATCGAGGAGTATGCTCATATGGATTTCATTATGGCTGGGAATGCCAATCAAAAAGTCTATGATCCTCTACTCACTTTCTTCGGGCAAAATTGA

Protein Analysis

398

Amino Acids

44.2

Weight (kDa)

6.3

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 37 - 92 5.4e-20 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 75 - 168 8.5e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000706)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03230 FvH4_5g03471 FvH4_5g03472 FvH4_6g41950 FvH4_6g41951
malus_domestica MD09G1115700.v1.1 MD09G1115900.v1.1 MD17G1107600.v1.1 MD17G1107700.v1.1
prunus_persica Prupe.3G208700_v2.0.a1 Prupe.3G208800_v2.0.a1 Prupe.5G037500_v2.0.a1
pyrus_communis pycom09g04010 pycom09g04030 pycom17g10020 pycom17g10030
rosa_chinensis RchiOBHm_Chr2g0101861 RchiOBHm_Chr2g0157451 RchiOBHm_Chr2g0157461 RchiOBHm_Chr2g0157841 RchiOBHm_Chr2g0157851 RchiOBHm_Chr3g0484721 RchiOBHm_Chr3g0484731 RchiOBHm_Chr3g0484741 RchiOBHm_Chr3g0484751 RchiOBHm_Chr6g0253931 RchiOBHm_Chr6g0253991
rosa_laevigata RLG00000014950 RLG00000014954 RLG00000020989 RLG00000021024 RLG00000021026 RLG00000021027 RLG00000021029 RLG00000021030
rosa_multiflora Rmu_sc0000195.1_g000023 Rmu_sc0000195.1_g000028 Rmu_sc0001689.1_g000007 Rmu_sc0001716.1_g000018 Rmu_sc0002441.1_g000012 Rmu_sc0004191.1_g000065 Rmu_sc0004473.1_g000004
rosa_roxburghii Rroxscaffold_1G00036760 Rroxscaffold_1G00036780 Rroxscaffold_2G00091900 Rroxscaffold_2G00092180 Rroxscaffold_2G00092200 Rroxscaffold_2G00127230 Rroxscaffold_7G00211340 Rroxscaffold_7G00211380
rosa_rugosa Rorug02G0102500 Rorug02G0206400 Rorug02G0206500 Rorug02G0461400 Rorug02G0464100 Rorug05G0548600 Rorug05G0548800
rosa_samantha Rh2BG155500 Rh2BG540100 Rh2BG540200 Rh2BG543500 Rh2BG543700 Rh2BG543800 Rh3CG296800 Rh6AG064700 Rh6AG065300
rosa_wichuraiana Rw2G020570 Rw2G043520 Rw2G043530 Rw2G043770 Rw2G043780 Rw3G023530 Rw3G023540 Rw6G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 936
AccBSI CCGCTC 1 cut(s) 212
AccII CGCG 1 cut(s) 363
AciI CCGC 3 cut(s) 210, 278, 363
AclWI GGATC 5 cut(s) 138, 383, 423, 523, 1157
AcoI YGGCCR 2 cut(s) 3, 493
AfaI GTAC 4 cut(s) 11, 382, 483, 962
AfiI CCNNNNNNNGG 1 cut(s) 728
AgsI TTSAA 2 cut(s) 18, 451
AjnI CCWGG 2 cut(s) 126, 772
Alw26I GTCTC 3 cut(s) 101, 713, 873
AlwI GGATC 5 cut(s) 138, 383, 423, 523, 1157
AlwNI CAGNNNCTG 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 164
AoxI GGCC 2 cut(s) 3, 493
ApeKI GCWGC 9 cut(s) 53, 56, 220, 268, 566, 608, 683, 786, 823
AspS9I GGNCC 1 cut(s) 776
AsuC2I CCSGG 2 cut(s) 713, 917
AsuHPI GGTGA 1 cut(s) 637
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 1 cut(s) 776
BalI TGGCCA 2 cut(s) 5, 495
BanII GRGCYC 1 cut(s) 696
BauI CACGAG 1 cut(s) 342
BbvI GCAGC 9 cut(s) 43, 65, 207, 255, 553, 595, 670, 773, 835
BccI CCATC 4 cut(s) 146, 190, 244, 248
BcgI CGANNNNNNTGC 2 cut(s) 1094, 1128
BciT130I CCWGG 2 cut(s) 128, 774
BclI TGATCA 2 cut(s) 76, 876
BcnI CCSGG 2 cut(s) 713, 917
BcoDI GTCTC 3 cut(s) 101, 713, 873
BfaI CTAG 3 cut(s) 101, 357, 740
BfmI CTRYAG 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 936
BisI GCNGC 9 cut(s) 54, 57, 221, 269, 567, 609, 684, 787, 824
BlpI GCTNAGC 1 cut(s) 617
BlsI GCNGC 9 cut(s) 55, 58, 222, 270, 568, 610, 685, 788, 825
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 4 cut(s) 128, 713, 774, 917
Bme18I GGWCC 1 cut(s) 776
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 776
BmrFI CCNGG 4 cut(s) 128, 713, 774, 917
BmrI ACTGGG 2 cut(s) 409, 617
BmsI GCATC 1 cut(s) 1009
BmuI ACTGGG 2 cut(s) 409, 617
Bpu1102I GCTNAGC 1 cut(s) 617
BpuMI CCSGG 2 cut(s) 713, 917
BsaI GGTCTC 1 cut(s) 713
BsaJI CCNNGG 1 cut(s) 163
BsaWI WCCGGW 1 cut(s) 955
Bsc4I CCNNNNNNNGG 1 cut(s) 728
Bse1I ACTGG 4 cut(s) 404, 475, 623, 709
BseBI CCWGG 2 cut(s) 128, 774
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 3 cut(s) 159, 259, 1036
BseLI CCNNNNNNNGG 1 cut(s) 728
BseMII CTCAG 3 cut(s) 227, 512, 578
BseNI ACTGG 4 cut(s) 404, 475, 623, 709
BseRI GAGGAG 3 cut(s) 119, 942, 1121
BseXI GCAGC 9 cut(s) 43, 65, 207, 255, 553, 595, 670, 773, 835
BseYI CCCAGC 1 cut(s) 1136
Bsh1236I CGCG 1 cut(s) 363
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 5, 495
BsiEI CGRYCG 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 4 cut(s) 440, 712, 917, 956
BslFI GGGAC 1 cut(s) 728
BslI CCNNNNNNNGG 1 cut(s) 728
BsmAI GTCTC 3 cut(s) 101, 713, 873
BsmFI GGGAC 1 cut(s) 728
BsmI GAATGC 2 cut(s) 975, 1147
BsnI GGCC 2 cut(s) 5, 495
Bso31I GGTCTC 1 cut(s) 713
BsoBI CYCGRG 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 696
Bsp1407I TGTACA 1 cut(s) 960
Bsp1720I GCTNAGC 1 cut(s) 617
BspACI CCGC 3 cut(s) 210, 278, 363
BspANI GGCC 2 cut(s) 5, 495
BspCNI CTCAG 3 cut(s) 226, 513, 579
BspFNI CGCG 1 cut(s) 363
BspHI TCATGA 1 cut(s) 1072
BspMI ACCTGC 1 cut(s) 936
BspPI GGATC 5 cut(s) 138, 383, 423, 523, 1157
BspTNI GGTCTC 1 cut(s) 713
BsrBI CCGCTC 1 cut(s) 212
BsrGI TGTACA 1 cut(s) 960
BsrI ACTGG 4 cut(s) 404, 475, 623, 709
BssECI CCNNGG 1 cut(s) 163
BssSI CACGAG 1 cut(s) 342
Bst2BI CACGAG 1 cut(s) 342
Bst2UI CCWGG 2 cut(s) 128, 774
Bst4CI ACNGT 2 cut(s) 71, 748
BstAUI TGTACA 1 cut(s) 960
BstC8I GCNNGC 3 cut(s) 333, 735, 973
BstDEI CTNAG 6 cut(s) 213, 377, 521, 587, 599, 617
BstENI CCTNNNNNAGG 1 cut(s) 726
BstF5I GGATG 3 cut(s) 159, 259, 1036
BstFNI CGCG 1 cut(s) 363
BstMAI GTCTC 3 cut(s) 101, 713, 873
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 5 cut(s) 53, 123, 241, 277, 1142
BstNI CCWGG 2 cut(s) 128, 774
BstSCI CCNGG 4 cut(s) 126, 711, 772, 915
BstSFI CTRYAG 1 cut(s) 833
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 9 cut(s) 43, 65, 207, 255, 553, 595, 670, 773, 835
BstXI CCANNNNNNTGG 1 cut(s) 399
BsuRI GGCC 2 cut(s) 5, 495
BtsCI GGATG 3 cut(s) 159, 259, 1036
BtsIMutI CAGTG 2 cut(s) 518, 702
BveI ACCTGC 1 cut(s) 936
Cac8I GCNNGC 3 cut(s) 333, 735, 973
CaiI CAGNNNCTG 1 cut(s) 220
CciI TCATGA 1 cut(s) 1072
Cfr13I GGNCC 1 cut(s) 776
Csp6I GTAC 4 cut(s) 10, 381, 482, 961
CviAII CATG 5 cut(s) 239, 264, 411, 967, 1073
CviQI GTAC 4 cut(s) 10, 381, 482, 961
DdeI CTNAG 6 cut(s) 213, 377, 521, 587, 599, 617
EaeI YGGCCR 2 cut(s) 3, 493
EciI GGCGGA 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 696
Eco31I GGTCTC 1 cut(s) 713
Eco47I GGWCC 1 cut(s) 776
Eco88I CYCGRG 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 726
EcoRII CCWGG 2 cut(s) 126, 772
EcoT38I GRGCYC 1 cut(s) 696
FaeI CATG 5 cut(s) 242, 267, 414, 970, 1076
FaqI GGGAC 1 cut(s) 728
FatI CATG 5 cut(s) 238, 263, 410, 966, 1072
FauNDI CATATG 2 cut(s) 679, 1119
FbaI TGATCA 2 cut(s) 76, 876
Fnu4HI GCNGC 9 cut(s) 54, 57, 221, 269, 567, 609, 684, 787, 824
FokI GGATG 3 cut(s) 146, 266, 1043
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 9 cut(s) 54, 57, 221, 269, 567, 609, 684, 787, 824
FspBI CTAG 3 cut(s) 101, 357, 740
GluI GCNGC 9 cut(s) 54, 57, 221, 269, 567, 609, 684, 787, 824
GsaI CCCAGC 1 cut(s) 1140
HaeIII GGCC 2 cut(s) 5, 495
HapII CCGG 4 cut(s) 440, 712, 917, 956
Hin1II CATG 5 cut(s) 242, 267, 414, 970, 1076
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 2 cut(s) 63, 178
HpaII CCGG 4 cut(s) 440, 712, 917, 956
HphI GGTGA 1 cut(s) 637
Hpy166II GTNNAC 4 cut(s) 460, 484, 961, 1036
Hpy188I TCNGA 4 cut(s) 216, 642, 981, 1030
Hpy188III TCNNGA 4 cut(s) 74, 186, 880, 1073
Hpy8I GTNNAC 4 cut(s) 460, 484, 961, 1036
HpyAV CCTTC 4 cut(s) 24, 107, 346, 1039
HpyCH4III ACNGT 2 cut(s) 71, 748
HpyCH4V TGCA 9 cut(s) 53, 59, 93, 172, 235, 754, 862, 931, 1022
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 123, 241, 277, 1142
HpyF3I CTNAG 6 cut(s) 213, 377, 521, 587, 599, 617
Hsp92II CATG 5 cut(s) 242, 267, 414, 970, 1076
Ksp22I TGATCA 2 cut(s) 76, 876
LmnI GCTCC 1 cut(s) 260
Lsp1109I GCAGC 9 cut(s) 43, 65, 207, 255, 553, 595, 670, 773, 835
LweI GCATC 1 cut(s) 1009
MaeI CTAG 3 cut(s) 101, 357, 740
MaeIII GTNAC 5 cut(s) 139, 155, 540, 573, 590
MbiI CCGCTC 1 cut(s) 212
MboII GAAGA 5 cut(s) 161, 428, 439, 991, 1171
MfeI CAATTG 1 cut(s) 419
MhlI GDGCHC 1 cut(s) 696
MlsI TGGCCA 2 cut(s) 5, 495
MluCI AATT 5 cut(s) 202, 370, 419, 755, 1189
MluNI TGGCCA 2 cut(s) 5, 495
Mox20I TGGCCA 2 cut(s) 5, 495
MscI TGGCCA 2 cut(s) 5, 495
MseI TTAA 2 cut(s) 924, 1056
Msp20I TGGCCA 2 cut(s) 5, 495
MspA1I CMGCKG 2 cut(s) 56, 608
MspI CCGG 4 cut(s) 440, 712, 917, 956
MspR9I CCNGG 4 cut(s) 128, 713, 774, 917
MunI CAATTG 1 cut(s) 419
Mva1269I GAATGC 2 cut(s) 975, 1147
MvaI CCWGG 2 cut(s) 128, 774
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 5 cut(s) 53, 123, 241, 277, 1142
NciI CCSGG 2 cut(s) 713, 917
NdeI CATATG 2 cut(s) 679, 1119
NlaIII CATG 5 cut(s) 242, 267, 414, 970, 1076
NmuCI GTSAC 1 cut(s) 573
PagI TCATGA 1 cut(s) 1072
PctI GAATGC 2 cut(s) 975, 1147
PfeI GAWTC 2 cut(s) 63, 178
PfoI TCCNGGA 1 cut(s) 711
PkrI GCNGC 9 cut(s) 55, 58, 222, 270, 568, 610, 685, 788, 825
Psp6I CCWGG 2 cut(s) 126, 772
PspFI CCCAGC 1 cut(s) 1136
PspGI CCWGG 2 cut(s) 126, 772
PspPI GGNCC 1 cut(s) 776
PstNI CAGNNNCTG 1 cut(s) 220
PvuII CAGCTG 2 cut(s) 56, 608
RsaI GTAC 4 cut(s) 11, 382, 483, 962
RsaNI GTAC 4 cut(s) 10, 381, 482, 961
SaqAI TTAA 2 cut(s) 924, 1056
SatI GCNGC 9 cut(s) 54, 57, 221, 269, 567, 609, 684, 787, 824
Sau96I GGNCC 1 cut(s) 776
ScaI AGTACT 1 cut(s) 382
ScrFI CCNGG 4 cut(s) 128, 713, 774, 917
SduI GDGCHC 1 cut(s) 696
SfaNI GCATC 1 cut(s) 1009
SfcI CTRYAG 1 cut(s) 833
SinI GGWCC 1 cut(s) 776
Sse9I AATT 5 cut(s) 202, 370, 419, 755, 1189
SsiI CCGC 3 cut(s) 210, 278, 363
SspMI CTAG 3 cut(s) 101, 357, 740
StyD4I CCNGG 4 cut(s) 126, 711, 772, 915
TaaI ACNGT 2 cut(s) 71, 748
TaqI TCGA 4 cut(s) 206, 673, 903, 1104
TasI AATT 5 cut(s) 202, 370, 419, 755, 1189
TatI WGTACW 3 cut(s) 380, 481, 960
TfiI GAWTC 2 cut(s) 63, 178
Tru1I TTAA 2 cut(s) 924, 1056
Tru9I TTAA 2 cut(s) 924, 1056
TscAI CASTG 2 cut(s) 525, 709
TseFI GTSAC 1 cut(s) 573
TseI GCWGC 9 cut(s) 53, 56, 220, 268, 566, 608, 683, 786, 823
Tsp45I GTSAC 1 cut(s) 573
TspDTI ATGAA 3 cut(s) 489, 543, 1117
TspRI CASTG 2 cut(s) 525, 709
VpaK11BI GGWCC 1 cut(s) 776
XagI CCTNNNNNAGG 1 cut(s) 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XspI CTAG 3 cut(s) 101, 357, 740
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.