Belongs to the AB hydrolase superfamily. Lipase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1029 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 505 |
| AccBSI | CCGCTC | 1 cut(s) | 215 |
| AccI | GTMKAC | 1 cut(s) | 1152 |
| AccII | CGCG | 1 cut(s) | 675 |
| AciI | CCGC | 4 cut(s) | 213, 264, 673, 761 |
| AclWI | GGATC | 3 cut(s) | 386, 426, 1151 |
| AcoI | YGGCCR | 3 cut(s) | 3, 496, 912 |
| AcsI | RAATTY | 1 cut(s) | 624 |
| AcyI | GRCGYC | 1 cut(s) | 1026 |
| AfaI | GTAC | 5 cut(s) | 11, 385, 486, 855, 956 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 318, 505, 689 |
| AgeI | ACCGGT | 1 cut(s) | 770 |
| AgsI | TTSAA | 2 cut(s) | 18, 804 |
| AleI | CACNNNNGTG | 1 cut(s) | 611 |
| AluBI | AGCT | 8 cut(s) | 223, 293, 527, 547, 569, 698, 940, 1081 |
| AluI | AGCT | 8 cut(s) | 223, 293, 527, 547, 569, 698, 940, 1081 |
| Alw26I | GTCTC | 2 cut(s) | 104, 707 |
| AlwI | GGATC | 3 cut(s) | 386, 426, 1151 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 223 |
| Ama87I | CYCGRG | 1 cut(s) | 345 |
| AoxI | GGCC | 3 cut(s) | 3, 496, 912 |
| ApeKI | GCWGC | 8 cut(s) | 56, 59, 223, 260, 428, 527, 569, 783 |
| ApoI | RAATTY | 1 cut(s) | 624 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 305, 337 |
| AsiGI | ACCGGT | 1 cut(s) | 770 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 625 |
| AspS9I | GGNCC | 1 cut(s) | 773 |
| AsuC2I | CCSGG | 2 cut(s) | 584, 735 |
| AsuHPI | GGTGA | 1 cut(s) | 598 |
| AsuII | TTCGAA | 1 cut(s) | 974 |
| AsuNHI | GCTAGC | 1 cut(s) | 359 |
| AvaI | CYCGRG | 1 cut(s) | 345 |
| AvaII | GGWCC | 1 cut(s) | 773 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 845, 878 |
| BalI | TGGCCA | 1 cut(s) | 498 |
| BbvI | GCAGC | 8 cut(s) | 43, 46, 210, 247, 415, 514, 556, 770 |
| BccI | CCATC | 5 cut(s) | 144, 149, 193, 247, 644 |
| BceAI | ACGGC | 1 cut(s) | 348 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 954, 988 |
| BciVI | GTATCC | 1 cut(s) | 680 |
| BclI | TGATCA | 3 cut(s) | 79, 870, 1063 |
| BcnI | CCSGG | 2 cut(s) | 584, 735 |
| BcoDI | GTCTC | 2 cut(s) | 104, 707 |
| BfaI | CTAG | 1 cut(s) | 360 |
| BfmI | CTRYAG | 1 cut(s) | 60 |
| BfuI | GTATCC | 1 cut(s) | 680 |
| BmcAI | AGTACT | 1 cut(s) | 385 |
| Bme1390I | CCNGG | 2 cut(s) | 584, 735 |
| Bme18I | GGWCC | 1 cut(s) | 773 |
| BmeT110I | CYCGRG | 1 cut(s) | 345 |
| BmgT120I | GGNCC | 1 cut(s) | 773 |
| BmrFI | CCNGG | 2 cut(s) | 584, 735 |
| BmrI | ACTGGG | 1 cut(s) | 515 |
| BmsI | GCATC | 2 cut(s) | 247, 1003 |
| BmtI | GCTAGC | 1 cut(s) | 363 |
| BmuI | ACTGGG | 1 cut(s) | 515 |
| BpmI | CTGGAG | 1 cut(s) | 267 |
| Bpu14I | TTCGAA | 1 cut(s) | 974 |
| BpuEI | CTTGAG | 1 cut(s) | 672 |
| BpuMI | CCSGG | 2 cut(s) | 584, 735 |
| BsaAI | YACGTR | 1 cut(s) | 1096 |
| BsaHI | GRCGYC | 1 cut(s) | 1026 |
| BsaJI | CCNNGG | 2 cut(s) | 166, 364 |
| BsaWI | WCCGGW | 2 cut(s) | 770, 949 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 318, 505, 689 |
| Bse118I | RCCGGY | 1 cut(s) | 770 |
| Bse1I | ACTGG | 2 cut(s) | 198, 510 |
| BseDI | CCNNGG | 2 cut(s) | 166, 364 |
| BseGI | GGATG | 2 cut(s) | 162, 262 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 318, 505, 689 |
| BseMII | CTCAG | 2 cut(s) | 230, 539 |
| BseNI | ACTGG | 2 cut(s) | 198, 510 |
| BseRI | GAGGAG | 3 cut(s) | 675, 936, 1115 |
| BseXI | GCAGC | 8 cut(s) | 43, 46, 210, 247, 415, 514, 556, 770 |
| Bsh1236I | CGCG | 1 cut(s) | 675 |
| Bsh1285I | CGRYCG | 1 cut(s) | 213 |
| BshFI | GGCC | 3 cut(s) | 5, 498, 914 |
| BshTI | ACCGGT | 1 cut(s) | 770 |
| BsiEI | CGRYCG | 1 cut(s) | 213 |
| BsiHKCI | CYCGRG | 1 cut(s) | 345 |
| BsiSI | CCGG | 5 cut(s) | 584, 734, 771, 911, 950 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 318, 505, 689 |
| BsmAI | GTCTC | 2 cut(s) | 104, 707 |
| BsmI | GAATGC | 1 cut(s) | 1141 |
| BsnI | GGCC | 3 cut(s) | 5, 498, 914 |
| BsoBI | CYCGRG | 1 cut(s) | 345 |
| Bsp119I | TTCGAA | 1 cut(s) | 974 |
| Bsp1407I | TGTACA | 1 cut(s) | 954 |
| BspACI | CCGC | 4 cut(s) | 213, 264, 673, 761 |
| BspANI | GGCC | 3 cut(s) | 5, 498, 914 |
| BspCNI | CTCAG | 2 cut(s) | 229, 540 |
| BspFNI | CGCG | 1 cut(s) | 675 |
| BspHI | TCATGA | 1 cut(s) | 1066 |
| BspMAI | CTGCAG | 1 cut(s) | 64 |
| BspOI | GCTAGC | 1 cut(s) | 363 |
| BspPI | GGATC | 3 cut(s) | 386, 426, 1151 |
| BspT104I | TTCGAA | 1 cut(s) | 974 |
| BsrBI | CCGCTC | 1 cut(s) | 215 |
| BsrFI | RCCGGY | 1 cut(s) | 770 |
| BsrGI | TGTACA | 1 cut(s) | 954 |
| BsrI | ACTGG | 2 cut(s) | 198, 510 |
| BssAI | RCCGGY | 1 cut(s) | 770 |
| BssECI | CCNNGG | 2 cut(s) | 166, 364 |
| BssNI | GRCGYC | 1 cut(s) | 1026 |
| BssT1I | CCWWGG | 1 cut(s) | 166 |
| Bst4CI | ACNGT | 2 cut(s) | 74, 613 |
| BstACI | GRCGYC | 1 cut(s) | 1026 |
| BstAUI | TGTACA | 1 cut(s) | 954 |
| BstBAI | YACGTR | 1 cut(s) | 1096 |
| BstBI | TTCGAA | 1 cut(s) | 974 |
| BstC8I | GCNNGC | 4 cut(s) | 336, 357, 361, 967 |
| BstDEI | CTNAG | 7 cut(s) | 216, 307, 380, 548, 560, 578, 1058 |
| BstDSI | CCRYGG | 1 cut(s) | 364 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 687 |
| BstF5I | GGATG | 2 cut(s) | 162, 262 |
| BstFNI | CGCG | 1 cut(s) | 675 |
| BstMAI | GTCTC | 2 cut(s) | 104, 707 |
| BstMCI | CGRYCG | 1 cut(s) | 213 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 56, 126 |
| BstNSI | RCATGY | 1 cut(s) | 925 |
| BstSCI | CCNGG | 2 cut(s) | 582, 733 |
| BstSFI | CTRYAG | 1 cut(s) | 60 |
| BstUI | CGCG | 1 cut(s) | 675 |
| BstV1I | GCAGC | 8 cut(s) | 43, 46, 210, 247, 415, 514, 556, 770 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 616, 757 |
| BsuI | GTATCC | 1 cut(s) | 680 |
| BsuRI | GGCC | 3 cut(s) | 5, 498, 914 |
| BtgI | CCRYGG | 1 cut(s) | 364 |
| BtsCI | GGATG | 2 cut(s) | 162, 262 |
| BtsIMutI | CAGTG | 2 cut(s) | 503, 618 |
| Cac8I | GCNNGC | 4 cut(s) | 336, 357, 361, 967 |
| CaiI | CAGNNNCTG | 1 cut(s) | 223 |
| CciI | TCATGA | 1 cut(s) | 1066 |
| Cfr10I | RCCGGY | 1 cut(s) | 770 |
| Cfr13I | GGNCC | 1 cut(s) | 773 |
| Csp6I | GTAC | 5 cut(s) | 10, 384, 485, 854, 955 |
| CspAI | ACCGGT | 1 cut(s) | 770 |
| CviAII | CATG | 6 cut(s) | 242, 267, 414, 922, 961, 1067 |
| CviQI | GTAC | 5 cut(s) | 10, 384, 485, 854, 955 |
| DdeI | CTNAG | 7 cut(s) | 216, 307, 380, 548, 560, 578, 1058 |
| EaeI | YGGCCR | 3 cut(s) | 3, 496, 912 |
| Eco130I | CCWWGG | 1 cut(s) | 166 |
| Eco47I | GGWCC | 1 cut(s) | 773 |
| Eco88I | CYCGRG | 1 cut(s) | 345 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 687 |
| EcoT14I | CCWWGG | 1 cut(s) | 166 |
| ErhI | CCWWGG | 1 cut(s) | 166 |
| FaeI | CATG | 6 cut(s) | 245, 270, 417, 925, 964, 1070 |
| FatI | CATG | 6 cut(s) | 241, 266, 413, 921, 960, 1066 |
| FauNDI | CATATG | 1 cut(s) | 1113 |
| FbaI | TGATCA | 3 cut(s) | 79, 870, 1063 |
| FblI | GTMKAC | 1 cut(s) | 1152 |
| FokI | GGATG | 2 cut(s) | 149, 269 |
| FspBI | CTAG | 1 cut(s) | 360 |
| GsuI | CTGGAG | 1 cut(s) | 267 |
| HaeIII | GGCC | 3 cut(s) | 5, 498, 914 |
| HapII | CCGG | 5 cut(s) | 584, 734, 771, 911, 950 |
| Hin1I | GRCGYC | 1 cut(s) | 1026 |
| Hin1II | CATG | 6 cut(s) | 245, 270, 417, 925, 964, 1070 |
| HindIII | AAGCTT | 2 cut(s) | 291, 1079 |
| HinfI | GANTC | 3 cut(s) | 66, 181, 654 |
| HpaII | CCGG | 5 cut(s) | 584, 734, 771, 911, 950 |
| HphI | GGTGA | 1 cut(s) | 598 |
| Hpy166II | GTNNAC | 6 cut(s) | 368, 463, 487, 742, 955, 1153 |
| Hpy188I | TCNGA | 5 cut(s) | 219, 396, 603, 865, 1024 |
| Hpy188III | TCNNGA | 5 cut(s) | 77, 189, 284, 874, 1067 |
| Hpy8I | GTNNAC | 6 cut(s) | 368, 463, 487, 742, 955, 1153 |
| HpyAV | CCTTC | 2 cut(s) | 24, 1033 |
| HpyCH4III | ACNGT | 2 cut(s) | 74, 613 |
| HpyCH4IV | ACGT | 4 cut(s) | 193, 981, 1026, 1095 |
| HpyCH4V | TGCA | 6 cut(s) | 62, 96, 175, 786, 925, 1016 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 56, 126 |
| HpyF3I | CTNAG | 7 cut(s) | 216, 307, 380, 548, 560, 578, 1058 |
| HpySE526I | ACGT | 4 cut(s) | 193, 981, 1026, 1095 |
| Hsp92I | GRCGYC | 1 cut(s) | 1026 |
| Hsp92II | CATG | 6 cut(s) | 245, 270, 417, 925, 964, 1070 |
| Ksp22I | TGATCA | 3 cut(s) | 79, 870, 1063 |
| LmnI | GCTCC | 1 cut(s) | 752 |
| Lsp1109I | GCAGC | 8 cut(s) | 43, 46, 210, 247, 415, 514, 556, 770 |
| LweI | GCATC | 2 cut(s) | 247, 1003 |
| MaeI | CTAG | 1 cut(s) | 360 |
| MaeII | ACGT | 4 cut(s) | 193, 981, 1026, 1095 |
| MaeIII | GTNAC | 6 cut(s) | 142, 158, 200, 534, 551, 766 |
| MbiI | CCGCTC | 1 cut(s) | 215 |
| MboII | GAAGA | 3 cut(s) | 164, 431, 1165 |
| MfeI | CAATTG | 1 cut(s) | 422 |
| MlsI | TGGCCA | 1 cut(s) | 498 |
| MluCI | AATT | 7 cut(s) | 205, 422, 454, 624, 751, 778, 904 |
| MluNI | TGGCCA | 1 cut(s) | 498 |
| MlyI | GAGTC | 1 cut(s) | 648 |
| MmeI | TCCRAC | 2 cut(s) | 419, 818 |
| Mox20I | TGGCCA | 1 cut(s) | 498 |
| MroXI | GAANNNNTTC | 1 cut(s) | 625 |
| MscI | TGGCCA | 1 cut(s) | 498 |
| MseI | TTAA | 3 cut(s) | 453, 918, 1050 |
| MslI | CAYNNNNRTG | 2 cut(s) | 611, 920 |
| Msp20I | TGGCCA | 1 cut(s) | 498 |
| MspA1I | CMGCKG | 1 cut(s) | 569 |
| MspI | CCGG | 5 cut(s) | 584, 734, 771, 911, 950 |
| MspR9I | CCNGG | 2 cut(s) | 584, 735 |
| MunI | CAATTG | 1 cut(s) | 422 |
| Mva1269I | GAATGC | 1 cut(s) | 1141 |
| MvnI | CGCG | 1 cut(s) | 675 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 56, 126 |
| NciI | CCSGG | 2 cut(s) | 584, 735 |
| NdeI | CATATG | 1 cut(s) | 1113 |
| NheI | GCTAGC | 1 cut(s) | 359 |
| NlaIII | CATG | 6 cut(s) | 245, 270, 417, 925, 964, 1070 |
| NmeAIII | GCCGAG | 1 cut(s) | 31 |
| NmuCI | GTSAC | 1 cut(s) | 534 |
| NspI | RCATGY | 1 cut(s) | 925 |
| NspV | TTCGAA | 1 cut(s) | 974 |
| OliI | CACNNNNGTG | 1 cut(s) | 611 |
| PaeR7I | CTCGAG | 1 cut(s) | 345 |
| PagI | TCATGA | 1 cut(s) | 1066 |
| PctI | GAATGC | 1 cut(s) | 1141 |
| PdmI | GAANNNNTTC | 1 cut(s) | 625 |
| PfeI | GAWTC | 2 cut(s) | 66, 181 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 505 |
| PinAI | ACCGGT | 1 cut(s) | 770 |
| PleI | GAGTC | 1 cut(s) | 648 |
| PpsI | GAGTC | 1 cut(s) | 648 |
| Ppu21I | YACGTR | 1 cut(s) | 1096 |
| PspPI | GGNCC | 1 cut(s) | 773 |
| PspXI | VCTCGAGB | 1 cut(s) | 345 |
| PstI | CTGCAG | 1 cut(s) | 64 |
| PstNI | CAGNNNCTG | 1 cut(s) | 223 |
| PvuII | CAGCTG | 1 cut(s) | 569 |
| RsaI | GTAC | 5 cut(s) | 11, 385, 486, 855, 956 |
| RsaNI | GTAC | 5 cut(s) | 10, 384, 485, 854, 955 |
| RseI | CAYNNNNRTG | 2 cut(s) | 611, 920 |
| SaqAI | TTAA | 3 cut(s) | 453, 918, 1050 |
| Sau96I | GGNCC | 1 cut(s) | 773 |
| ScaI | AGTACT | 1 cut(s) | 385 |
| SchI | GAGTC | 1 cut(s) | 648 |
| ScrFI | CCNGG | 2 cut(s) | 584, 735 |
| SfaNI | GCATC | 2 cut(s) | 247, 1003 |
| SfcI | CTRYAG | 1 cut(s) | 60 |
| Sfr274I | CTCGAG | 1 cut(s) | 345 |
| SfuI | TTCGAA | 1 cut(s) | 974 |
| SinI | GGWCC | 1 cut(s) | 773 |
| SlaI | CTCGAG | 1 cut(s) | 345 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 611, 920 |
| SmlI | CTYRAG | 2 cut(s) | 345, 687 |
| SmoI | CTYRAG | 2 cut(s) | 345, 687 |
| Sse9I | AATT | 7 cut(s) | 205, 422, 454, 624, 751, 778, 904 |
| SsiI | CCGC | 4 cut(s) | 213, 264, 673, 761 |
| SspMI | CTAG | 1 cut(s) | 360 |
| StyD4I | CCNGG | 2 cut(s) | 582, 733 |
| StyI | CCWWGG | 1 cut(s) | 166 |
| TaaI | ACNGT | 2 cut(s) | 74, 613 |
| TaiI | ACGT | 4 cut(s) | 196, 984, 1029, 1098 |
| TaqI | TCGA | 3 cut(s) | 209, 346, 974 |
| TasI | AATT | 7 cut(s) | 205, 422, 454, 624, 751, 778, 904 |
| TatI | WGTACW | 3 cut(s) | 383, 484, 954 |
| TauI | GCSGC | 2 cut(s) | 266, 764 |
| TfiI | GAWTC | 2 cut(s) | 66, 181 |
| Tru1I | TTAA | 3 cut(s) | 453, 918, 1050 |
| Tru9I | TTAA | 3 cut(s) | 453, 918, 1050 |
| TscAI | CASTG | 2 cut(s) | 510, 618 |
| TseFI | GTSAC | 1 cut(s) | 534 |
| TseI | GCWGC | 8 cut(s) | 56, 59, 223, 260, 428, 527, 569, 783 |
| Tsp45I | GTSAC | 1 cut(s) | 534 |
| TspDTI | ATGAA | 1 cut(s) | 1111 |
| TspRI | CASTG | 2 cut(s) | 510, 618 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 505 |
| VpaK11BI | GGWCC | 1 cut(s) | 773 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 687 |
| XapI | RAATTY | 1 cut(s) | 624 |
| XceI | RCATGY | 1 cut(s) | 925 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 847 |
| XhoI | CTCGAG | 1 cut(s) | 345 |
| XmiI | GTMKAC | 1 cut(s) | 1152 |
| XmnI | GAANNNNTTC | 1 cut(s) | 625 |
| XspI | CTAG | 1 cut(s) | 360 |
| ZraI | GACGTC | 1 cut(s) | 1027 |
| ZrmI | AGTACT | 1 cut(s) | 385 |