Belongs to the AB hydrolase superfamily. Lipase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 233, 263, 623, 920 |
| AclI | AACGTT | 1 cut(s) | 521 |
| AclWI | GGATC | 4 cut(s) | 231, 303, 395, 408 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 3 cut(s) | 186, 686, 928 |
| AfaI | GTAC | 5 cut(s) | 11, 394, 864, 965, 1292 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 698 |
| AgsI | TTSAA | 1 cut(s) | 463 |
| AjnI | CCWGG | 3 cut(s) | 218, 742, 1265 |
| AluBI | AGCT | 7 cut(s) | 20, 433, 536, 556, 578, 1052, 1090 |
| AluI | AGCT | 7 cut(s) | 20, 433, 536, 556, 578, 1052, 1090 |
| Alw26I | GTCTC | 2 cut(s) | 110, 876 |
| AlwI | GGATC | 4 cut(s) | 231, 303, 395, 408 |
| Ama87I | CYCGRG | 2 cut(s) | 173, 354 |
| AoxI | GGCC | 1 cut(s) | 3 |
| ApeKI | GCWGC | 4 cut(s) | 269, 536, 578, 934 |
| ApoI | RAATTY | 3 cut(s) | 186, 686, 928 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 61, 93, 314, 346 |
| AspS9I | GGNCC | 1 cut(s) | 782 |
| AsuHPI | GGTGA | 1 cut(s) | 1265 |
| AvaI | CYCGRG | 2 cut(s) | 173, 354 |
| AvaII | GGWCC | 1 cut(s) | 782 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BamHI | GGATCC | 1 cut(s) | 400 |
| BauI | CACGAG | 1 cut(s) | 120 |
| BbsI | GAAGAC | 2 cut(s) | 165, 671 |
| BbvI | GCAGC | 4 cut(s) | 281, 523, 565, 946 |
| BceAI | ACGGC | 1 cut(s) | 357 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 926, 960 |
| BciT130I | CCWGG | 3 cut(s) | 220, 744, 1267 |
| BclI | TGATCA | 2 cut(s) | 826, 879 |
| BcoDI | GTCTC | 2 cut(s) | 110, 876 |
| BfaI | CTAG | 6 cut(s) | 21, 44, 260, 369, 533, 698 |
| BfmI | CTRYAG | 1 cut(s) | 719 |
| BglII | AGATCT | 1 cut(s) | 814 |
| BisI | GCNGC | 5 cut(s) | 233, 270, 537, 579, 935 |
| BlpI | GCTNAGC | 2 cut(s) | 587, 657 |
| BlsI | GCNGC | 5 cut(s) | 234, 271, 538, 580, 936 |
| BmcAI | AGTACT | 1 cut(s) | 394 |
| Bme1390I | CCNGG | 3 cut(s) | 220, 744, 1267 |
| Bme18I | GGWCC | 1 cut(s) | 782 |
| BmeT110I | CYCGRG | 2 cut(s) | 173, 354 |
| BmgT120I | GGNCC | 1 cut(s) | 782 |
| BmiI | GGNNCC | 2 cut(s) | 402, 892 |
| BmrFI | CCNGG | 3 cut(s) | 220, 744, 1267 |
| BmsI | GCATC | 4 cut(s) | 151, 256, 281, 1012 |
| BoxI | GACNNNNGTC | 1 cut(s) | 490 |
| BpiI | GAAGAC | 2 cut(s) | 165, 671 |
| BpmI | CTGGAG | 1 cut(s) | 1296 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 587, 657 |
| BsaAI | YACGTR | 2 cut(s) | 148, 1105 |
| BsaJI | CCNNGG | 4 cut(s) | 219, 373, 1003, 1266 |
| BsaWI | WCCGGW | 1 cut(s) | 958 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 698 |
| Bse1I | ACTGG | 1 cut(s) | 422 |
| BseBI | CCWGG | 3 cut(s) | 220, 744, 1267 |
| BseDI | CCNNGG | 4 cut(s) | 219, 373, 1003, 1266 |
| BseGI | GGATG | 2 cut(s) | 271, 739 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 698 |
| BseMII | CTCAG | 3 cut(s) | 548, 648, 887 |
| BseNI | ACTGG | 1 cut(s) | 422 |
| BseRI | GAGGAG | 4 cut(s) | 684, 945, 1124, 1271 |
| BseXI | GCAGC | 4 cut(s) | 281, 523, 565, 946 |
| BshFI | GGCC | 1 cut(s) | 5 |
| BsiHKCI | CYCGRG | 2 cut(s) | 173, 354 |
| BsiSI | CCGG | 1 cut(s) | 959 |
| BslFI | GGGAC | 2 cut(s) | 428, 529 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 698 |
| BsmAI | GTCTC | 2 cut(s) | 110, 876 |
| BsmFI | GGGAC | 2 cut(s) | 428, 529 |
| BsmI | GAATGC | 1 cut(s) | 1150 |
| BsnI | GGCC | 1 cut(s) | 5 |
| BsoBI | CYCGRG | 2 cut(s) | 173, 354 |
| Bsp1407I | TGTACA | 1 cut(s) | 963 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 587, 657 |
| BspACI | CCGC | 4 cut(s) | 233, 263, 623, 920 |
| BspANI | GGCC | 1 cut(s) | 5 |
| BspCNI | CTCAG | 3 cut(s) | 549, 649, 886 |
| BspHI | TCATGA | 1 cut(s) | 1075 |
| BspLI | GGNNCC | 2 cut(s) | 402, 892 |
| BspMAI | CTGCAG | 1 cut(s) | 723 |
| BspPI | GGATC | 4 cut(s) | 231, 303, 395, 408 |
| BsrGI | TGTACA | 1 cut(s) | 963 |
| BsrI | ACTGG | 1 cut(s) | 422 |
| BssECI | CCNNGG | 4 cut(s) | 219, 373, 1003, 1266 |
| BssSI | CACGAG | 1 cut(s) | 120 |
| Bst2BI | CACGAG | 1 cut(s) | 120 |
| Bst2UI | CCWGG | 3 cut(s) | 220, 744, 1267 |
| Bst4CI | ACNGT | 3 cut(s) | 164, 201, 1175 |
| Bst6I | CTCTTC | 2 cut(s) | 921, 1230 |
| BstAUI | TGTACA | 1 cut(s) | 963 |
| BstBAI | YACGTR | 2 cut(s) | 148, 1105 |
| BstC8I | GCNNGC | 2 cut(s) | 345, 435 |
| BstDEI | CTNAG | 9 cut(s) | 182, 389, 396, 557, 569, 587, 657, 873, 1180 |
| BstDSI | CCRYGG | 1 cut(s) | 373 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 696 |
| BstF5I | GGATG | 2 cut(s) | 271, 739 |
| BstMAI | GTCTC | 2 cut(s) | 110, 876 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 253, 269, 753 |
| BstNI | CCWGG | 3 cut(s) | 220, 744, 1267 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 490 |
| BstSCI | CCNGG | 3 cut(s) | 218, 742, 1265 |
| BstSFI | CTRYAG | 1 cut(s) | 719 |
| BstSNI | TACGTA | 1 cut(s) | 1105 |
| BstV1I | GCAGC | 4 cut(s) | 281, 523, 565, 946 |
| BstV2I | GAAGAC | 2 cut(s) | 165, 671 |
| BstX2I | RGATCY | 2 cut(s) | 400, 814 |
| BstYI | RGATCY | 2 cut(s) | 400, 814 |
| BsuRI | GGCC | 1 cut(s) | 5 |
| BtgI | CCRYGG | 1 cut(s) | 373 |
| BtsCI | GGATG | 2 cut(s) | 271, 739 |
| BtsIMutI | CAGTG | 1 cut(s) | 1180 |
| Cac8I | GCNNGC | 2 cut(s) | 345, 435 |
| CciI | TCATGA | 1 cut(s) | 1075 |
| Cfr13I | GGNCC | 1 cut(s) | 782 |
| Csp6I | GTAC | 5 cut(s) | 10, 393, 863, 964, 1291 |
| CviAII | CATG | 8 cut(s) | 194, 251, 276, 423, 970, 1076, 1160, 1294 |
| CviQI | GTAC | 5 cut(s) | 10, 393, 863, 964, 1291 |
| DdeI | CTNAG | 9 cut(s) | 182, 389, 396, 557, 569, 587, 657, 873, 1180 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 2 cut(s) | 921, 1230 |
| EarI | CTCTTC | 2 cut(s) | 921, 1230 |
| Eco105I | TACGTA | 1 cut(s) | 1105 |
| Eco47I | GGWCC | 1 cut(s) | 782 |
| Eco88I | CYCGRG | 2 cut(s) | 173, 354 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 696 |
| EcoRI | GAATTC | 1 cut(s) | 186 |
| EcoRII | CCWGG | 3 cut(s) | 218, 742, 1265 |
| FaeI | CATG | 8 cut(s) | 197, 254, 279, 426, 973, 1079, 1163, 1297 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1041, 1073 |
| FaqI | GGGAC | 2 cut(s) | 428, 529 |
| FatI | CATG | 8 cut(s) | 193, 250, 275, 422, 969, 1075, 1159, 1293 |
| FauNDI | CATATG | 1 cut(s) | 1122 |
| FbaI | TGATCA | 2 cut(s) | 826, 879 |
| Fnu4HI | GCNGC | 5 cut(s) | 233, 270, 537, 579, 935 |
| FokI | GGATG | 2 cut(s) | 278, 726 |
| Fsp4HI | GCNGC | 5 cut(s) | 233, 270, 537, 579, 935 |
| FspBI | CTAG | 6 cut(s) | 21, 44, 260, 369, 533, 698 |
| GluI | GCNGC | 5 cut(s) | 233, 270, 537, 579, 935 |
| GsuI | CTGGAG | 1 cut(s) | 1296 |
| HaeIII | GGCC | 1 cut(s) | 5 |
| HapII | CCGG | 1 cut(s) | 959 |
| Hin1II | CATG | 8 cut(s) | 197, 254, 279, 426, 973, 1079, 1163, 1297 |
| HincII | GTYRAC | 1 cut(s) | 525 |
| HindII | GTYRAC | 1 cut(s) | 525 |
| HindIII | AAGCTT | 2 cut(s) | 1050, 1088 |
| HinfI | GANTC | 2 cut(s) | 365, 1163 |
| HpaII | CCGG | 1 cut(s) | 959 |
| HphI | GGTGA | 1 cut(s) | 1265 |
| Hpy166II | GTNNAC | 5 cut(s) | 377, 525, 751, 951, 964 |
| Hpy188I | TCNGA | 5 cut(s) | 228, 876, 884, 1033, 1168 |
| Hpy188III | TCNNGA | 6 cut(s) | 57, 80, 137, 683, 1076, 1275 |
| Hpy8I | GTNNAC | 5 cut(s) | 377, 525, 751, 951, 964 |
| HpyAV | CCTTC | 2 cut(s) | 24, 813 |
| HpyCH4III | ACNGT | 3 cut(s) | 164, 201, 1175 |
| HpyCH4IV | ACGT | 4 cut(s) | 147, 521, 1104, 1207 |
| HpyCH4V | TGCA | 7 cut(s) | 102, 247, 269, 721, 735, 934, 1025 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 253, 269, 753 |
| HpyF3I | CTNAG | 9 cut(s) | 182, 389, 396, 557, 569, 587, 657, 873, 1180 |
| HpySE526I | ACGT | 4 cut(s) | 147, 521, 1104, 1207 |
| Hsp92II | CATG | 8 cut(s) | 197, 254, 279, 426, 973, 1079, 1163, 1297 |
| Ksp22I | TGATCA | 2 cut(s) | 826, 879 |
| Lsp1109I | GCAGC | 4 cut(s) | 281, 523, 565, 946 |
| LweI | GCATC | 4 cut(s) | 151, 256, 281, 1012 |
| MaeI | CTAG | 6 cut(s) | 21, 44, 260, 369, 533, 698 |
| MaeII | ACGT | 4 cut(s) | 147, 521, 1104, 1207 |
| MaeIII | GTNAC | 5 cut(s) | 61, 148, 164, 492, 1175 |
| MboII | GAAGA | 5 cut(s) | 170, 440, 671, 908, 1247 |
| MfeI | CAATTG | 1 cut(s) | 594 |
| MflI | RGATCY | 2 cut(s) | 400, 814 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 8 cut(s) | 186, 463, 594, 662, 686, 726, 787, 928 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 1 cut(s) | 359 |
| MmeI | TCCRAC | 1 cut(s) | 1121 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 2 cut(s) | 629, 1300 |
| MslI | CAYNNNNRTG | 1 cut(s) | 899 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspA1I | CMGCKG | 2 cut(s) | 433, 578 |
| MspI | CCGG | 1 cut(s) | 959 |
| MspR9I | CCNGG | 3 cut(s) | 220, 744, 1267 |
| MunI | CAATTG | 1 cut(s) | 594 |
| Mva1269I | GAATGC | 1 cut(s) | 1150 |
| MvaI | CCWGG | 3 cut(s) | 220, 744, 1267 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 253, 269, 753 |
| NdeI | CATATG | 1 cut(s) | 1122 |
| NlaIII | CATG | 8 cut(s) | 197, 254, 279, 426, 973, 1079, 1163, 1297 |
| NlaIV | GGNNCC | 2 cut(s) | 402, 892 |
| NmuCI | GTSAC | 2 cut(s) | 492, 1175 |
| PaeR7I | CTCGAG | 1 cut(s) | 354 |
| PagI | TCATGA | 1 cut(s) | 1075 |
| PctI | GAATGC | 1 cut(s) | 1150 |
| PfeI | GAWTC | 1 cut(s) | 1163 |
| PkrI | GCNGC | 5 cut(s) | 234, 271, 538, 580, 936 |
| PleI | GAGTC | 1 cut(s) | 359 |
| PpsI | GAGTC | 1 cut(s) | 359 |
| Ppu21I | YACGTR | 2 cut(s) | 148, 1105 |
| PshAI | GACNNNNGTC | 1 cut(s) | 490 |
| Psp1406I | AACGTT | 1 cut(s) | 521 |
| Psp6I | CCWGG | 3 cut(s) | 218, 742, 1265 |
| PspGI | CCWGG | 3 cut(s) | 218, 742, 1265 |
| PspN4I | GGNNCC | 2 cut(s) | 402, 892 |
| PspPI | GGNCC | 1 cut(s) | 782 |
| PspXI | VCTCGAGB | 1 cut(s) | 354 |
| PstI | CTGCAG | 1 cut(s) | 723 |
| PsuI | RGATCY | 2 cut(s) | 400, 814 |
| PvuII | CAGCTG | 2 cut(s) | 433, 578 |
| RsaI | GTAC | 5 cut(s) | 11, 394, 864, 965, 1292 |
| RsaNI | GTAC | 5 cut(s) | 10, 393, 863, 964, 1291 |
| RseI | CAYNNNNRTG | 1 cut(s) | 899 |
| SaqAI | TTAA | 2 cut(s) | 629, 1300 |
| SatI | GCNGC | 5 cut(s) | 233, 270, 537, 579, 935 |
| Sau96I | GGNCC | 1 cut(s) | 782 |
| ScaI | AGTACT | 1 cut(s) | 394 |
| SchI | GAGTC | 1 cut(s) | 359 |
| ScrFI | CCNGG | 3 cut(s) | 220, 744, 1267 |
| SfaNI | GCATC | 4 cut(s) | 151, 256, 281, 1012 |
| SfcI | CTRYAG | 1 cut(s) | 719 |
| Sfr274I | CTCGAG | 1 cut(s) | 354 |
| SinI | GGWCC | 1 cut(s) | 782 |
| SlaI | CTCGAG | 1 cut(s) | 354 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 899 |
| SmlI | CTYRAG | 1 cut(s) | 354 |
| SmoI | CTYRAG | 1 cut(s) | 354 |
| SnaBI | TACGTA | 1 cut(s) | 1105 |
| Sse9I | AATT | 8 cut(s) | 186, 463, 594, 662, 686, 726, 787, 928 |
| SsiI | CCGC | 4 cut(s) | 233, 263, 623, 920 |
| SspI | AATATT | 1 cut(s) | 979 |
| SspMI | CTAG | 6 cut(s) | 21, 44, 260, 369, 533, 698 |
| StyD4I | CCNGG | 3 cut(s) | 218, 742, 1265 |
| TaaI | ACNGT | 3 cut(s) | 164, 201, 1175 |
| TaiI | ACGT | 4 cut(s) | 150, 524, 1107, 1210 |
| TaqI | TCGA | 3 cut(s) | 215, 355, 906 |
| TasI | AATT | 8 cut(s) | 186, 463, 594, 662, 686, 726, 787, 928 |
| TatI | WGTACW | 2 cut(s) | 392, 963 |
| TauI | GCSGC | 1 cut(s) | 235 |
| TfiI | GAWTC | 1 cut(s) | 1163 |
| Tru1I | TTAA | 2 cut(s) | 629, 1300 |
| Tru9I | TTAA | 2 cut(s) | 629, 1300 |
| TscAI | CASTG | 1 cut(s) | 1180 |
| TseFI | GTSAC | 2 cut(s) | 492, 1175 |
| TseI | GCWGC | 4 cut(s) | 269, 536, 578, 934 |
| Tsp45I | GTSAC | 2 cut(s) | 492, 1175 |
| TspDTI | ATGAA | 3 cut(s) | 301, 501, 1120 |
| TspGWI | ACGGA | 1 cut(s) | 377 |
| TspRI | CASTG | 1 cut(s) | 1180 |
| VpaK11BI | GGWCC | 1 cut(s) | 782 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 696 |
| XapI | RAATTY | 3 cut(s) | 186, 686, 928 |
| XhoI | CTCGAG | 1 cut(s) | 354 |
| XspI | CTAG | 6 cut(s) | 21, 44, 260, 369, 533, 698 |
| ZrmI | AGTACT | 1 cut(s) | 394 |