RchiOBHm_Chr3g0484741
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000706
Physical Location & Seq
Forward (+)
31178053 .. 31179856
1804 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44945

Sequence Viewer

Length: 1098 bp
ATGTTGACGCTTCTGATGGGAACTATATCTAGATATGATGGAATATTTCAGGTAACGACAGAAGATGGTTACATCCTCGGATTGCAGAGGATTCCATCTGGACGTTCCAGTAACAATTCGACCTCTCAGAAGCTGCCTGTTTTATTACAACATGGACTTACGATGGATGCTGCAGCATGGCTAATCAATCCTCCAGATCAAGCTTTGGCGTTTATCTTAGCCGACAAGGGGTTTGATGTATGGCTTGCTAATGCTCGAGGAACGCAGGCTAGCCGTGGACACCAATCACTTAGTACTAATGATCCGACTTATTGGGAATGGACATGGGATCAATTGGCTACTTATGATCTTCCTGCTTTCTATAATTATGTGAACAATCAAACAGGACAGAAAGTACACTATGTTGGCCATTCACTGGGAACATTGTCAGTATTAGCTGCCTTGTCACAACAAAAGCTGAGTAACACACTAAGATCAGCTGCTTTACTTAGCCCGGTTGCTTATCTGGGTCAGATCACCACAATGTTGGGAAAAATTTCTGCTCAATCACATTCTGCTGATGGACTCTACGCCTCTGGTATCCGCGAATATCCTCCTCAAGGGGTAGCTGGACAGAACTTGGAGACATTTCTCTGCTCACAACCGGGTGTGGACTGCTCCAATTTTATGGCGGCTGTAACCGGTCCTAATTGCTGCATAACTCCAACAAGTTCAAATCTTATCTTTCAGCATATACCACAGTCCACTTCCACAAAAAATATGGTACATTTGTCTGAAATGATCAGGAGAGGAACAATAACAATGTTTGATTACAATTTTCCGGCCATTAACATGCAACACTACAATCAGCTTACTCCTCCGGTGTACAACATGGCAAGCATTCCGAAAGATGTTCCACTTTTCTTGGGCTATGGAGGGAGAGATGCACTTTCGGACGTCAATGATGTGAAGGCTTTGCTTAATGACCTTAGTGATCATGACAAGGACAAGCTTGTGGAACAATACGTGGAGGAGTATGCTCATATGGATTTCATTATGGGAGGGAATGCCAATCAAAAAGTCTACGATCCTCTGCTCACTTTCTTCGGGCAGCATTGA

Protein Analysis

365

Amino Acids

40.23

Weight (kDa)

5.58

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 16 - 63 1.1e-16 Partial alpha/beta-hydrolase lipase region
Abhydrolase_1 PF00561 46 - 165 1.6e-10 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000706)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03230 FvH4_5g03471 FvH4_5g03472 FvH4_6g41950 FvH4_6g41951
malus_domestica MD09G1115700.v1.1 MD09G1115900.v1.1 MD17G1107600.v1.1 MD17G1107700.v1.1
prunus_persica Prupe.3G208700_v2.0.a1 Prupe.3G208800_v2.0.a1 Prupe.5G037500_v2.0.a1
pyrus_communis pycom09g04010 pycom09g04030 pycom17g10020 pycom17g10030
rosa_chinensis RchiOBHm_Chr2g0101861 RchiOBHm_Chr2g0157451 RchiOBHm_Chr2g0157461 RchiOBHm_Chr2g0157841 RchiOBHm_Chr2g0157851 RchiOBHm_Chr3g0484721 RchiOBHm_Chr3g0484731 RchiOBHm_Chr3g0484741 RchiOBHm_Chr3g0484751 RchiOBHm_Chr6g0253931 RchiOBHm_Chr6g0253991
rosa_laevigata RLG00000014950 RLG00000014954 RLG00000020989 RLG00000021024 RLG00000021026 RLG00000021027 RLG00000021029 RLG00000021030
rosa_multiflora Rmu_sc0000195.1_g000023 Rmu_sc0000195.1_g000028 Rmu_sc0001689.1_g000007 Rmu_sc0001716.1_g000018 Rmu_sc0002441.1_g000012 Rmu_sc0004191.1_g000065 Rmu_sc0004473.1_g000004
rosa_roxburghii Rroxscaffold_1G00036760 Rroxscaffold_1G00036780 Rroxscaffold_2G00091900 Rroxscaffold_2G00092180 Rroxscaffold_2G00092200 Rroxscaffold_2G00127230 Rroxscaffold_7G00211340 Rroxscaffold_7G00211380
rosa_rugosa Rorug02G0102500 Rorug02G0206400 Rorug02G0206500 Rorug02G0461400 Rorug02G0464100 Rorug05G0548600 Rorug05G0548800
rosa_samantha Rh2BG155500 Rh2BG540100 Rh2BG540200 Rh2BG543500 Rh2BG543700 Rh2BG543800 Rh3CG296800 Rh6AG064700 Rh6AG065300
rosa_wichuraiana Rw2G020570 Rw2G043520 Rw2G043530 Rw2G043770 Rw2G043780 Rw3G023530 Rw3G023540 Rw6G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 939
AccB7I CCANNNNNTGG 1 cut(s) 415
AccI GTMKAC 1 cut(s) 1062
AccII CGCG 1 cut(s) 585
AciI CCGC 2 cut(s) 583, 671
AclWI GGATC 3 cut(s) 296, 336, 1061
AcoI YGGCCR 2 cut(s) 406, 822
AcsI RAATTY 1 cut(s) 534
AcyI GRCGYC 1 cut(s) 936
AfaI GTAC 4 cut(s) 295, 396, 765, 866
AfiI CCNNNNNNNGG 3 cut(s) 228, 415, 599
AgeI ACCGGT 1 cut(s) 680
AgsI TTSAA 1 cut(s) 714
AluBI AGCT 8 cut(s) 133, 203, 437, 457, 479, 608, 850, 991
AluI AGCT 8 cut(s) 133, 203, 437, 457, 479, 608, 850, 991
Alw26I GTCTC 1 cut(s) 617
AlwI GGATC 3 cut(s) 296, 336, 1061
AlwNI CAGNNNCTG 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 2 cut(s) 406, 822
ApeKI GCWGC 7 cut(s) 133, 170, 173, 437, 479, 693, 1090
ApoI RAATTY 1 cut(s) 534
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AsiGI ACCGGT 1 cut(s) 680
Asp700I GAANNNNTTC 1 cut(s) 535
AspS9I GGNCC 1 cut(s) 683
AsuC2I CCSGG 2 cut(s) 494, 645
AsuHPI GGTGA 1 cut(s) 508
AsuNHI GCTAGC 1 cut(s) 269
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 1 cut(s) 683
BaeI ACNNNNGTAYC 2 cut(s) 755, 788
BalI TGGCCA 1 cut(s) 408
BbvI GCAGC 6 cut(s) 120, 157, 185, 424, 466, 680
BccI CCATC 6 cut(s) 10, 32, 59, 103, 157, 554
BceAI ACGGC 1 cut(s) 258
BciVI GTATCC 1 cut(s) 590
BclI TGATCA 2 cut(s) 780, 973
BcnI CCSGG 2 cut(s) 494, 645
BcoDI GTCTC 1 cut(s) 617
BfaI CTAG 2 cut(s) 30, 270
BfmI CTRYAG 1 cut(s) 171
BfuI GTATCC 1 cut(s) 590
BisI GCNGC 8 cut(s) 134, 171, 174, 438, 480, 672, 694, 1091
BlsI GCNGC 8 cut(s) 135, 172, 175, 439, 481, 673, 695, 1092
BmcAI AGTACT 1 cut(s) 295
Bme1390I CCNGG 2 cut(s) 494, 645
Bme18I GGWCC 1 cut(s) 683
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 683
BmrFI CCNGG 2 cut(s) 494, 645
BmrI ACTGGG 1 cut(s) 425
BmsI GCATC 2 cut(s) 157, 913
BmtI GCTAGC 1 cut(s) 273
BmuI ACTGGG 1 cut(s) 425
BpmI CTGGAG 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 582
BpuMI CCSGG 2 cut(s) 494, 645
BsaAI YACGTR 1 cut(s) 1006
BsaHI GRCGYC 1 cut(s) 936
BsaJI CCNNGG 2 cut(s) 76, 274
BsaWI WCCGGW 2 cut(s) 680, 859
Bsc4I CCNNNNNNNGG 3 cut(s) 228, 415, 599
Bse118I RCCGGY 1 cut(s) 680
Bse1I ACTGG 2 cut(s) 108, 420
BseDI CCNNGG 2 cut(s) 76, 274
BseGI GGATG 2 cut(s) 72, 172
BseLI CCNNNNNNNGG 3 cut(s) 228, 415, 599
BseMII CTCAG 2 cut(s) 140, 449
BseNI ACTGG 2 cut(s) 108, 420
BseRI GAGGAG 3 cut(s) 585, 846, 1025
BseXI GCAGC 6 cut(s) 120, 157, 185, 424, 466, 680
Bsh1236I CGCG 1 cut(s) 585
BshFI GGCC 2 cut(s) 408, 824
BshTI ACCGGT 1 cut(s) 680
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 5 cut(s) 494, 644, 681, 821, 860
BslI CCNNNNNNNGG 3 cut(s) 228, 415, 599
BsmAI GTCTC 1 cut(s) 617
BsmI GAATGC 2 cut(s) 879, 1051
BsnI GGCC 2 cut(s) 408, 824
BsoBI CYCGRG 1 cut(s) 255
Bsp1407I TGTACA 1 cut(s) 864
Bsp143I GATC 9 cut(s) 196, 301, 328, 346, 473, 513, 780, 973, 1066
BspACI CCGC 2 cut(s) 583, 671
BspANI GGCC 2 cut(s) 408, 824
BspCNI CTCAG 2 cut(s) 139, 450
BspFNI CGCG 1 cut(s) 585
BspHI TCATGA 1 cut(s) 976
BspMAI CTGCAG 1 cut(s) 175
BspOI GCTAGC 1 cut(s) 273
BspPI GGATC 3 cut(s) 296, 336, 1061
BsrFI RCCGGY 1 cut(s) 680
BsrGI TGTACA 1 cut(s) 864
BsrI ACTGG 2 cut(s) 108, 420
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 2 cut(s) 76, 274
BssMI GATC 9 cut(s) 196, 301, 328, 346, 473, 513, 780, 973, 1066
BssNI GRCGYC 1 cut(s) 936
Bst4CI ACNGT 1 cut(s) 741
BstACI GRCGYC 1 cut(s) 936
BstAUI TGTACA 1 cut(s) 864
BstBAI YACGTR 1 cut(s) 1006
BstC8I GCNNGC 4 cut(s) 246, 267, 271, 877
BstDEI CTNAG 7 cut(s) 126, 217, 290, 458, 470, 488, 968
BstDSI CCRYGG 1 cut(s) 274
BstENI CCTNNNNNAGG 1 cut(s) 597
BstF5I GGATG 2 cut(s) 72, 172
BstFNI CGCG 1 cut(s) 585
BstKTI GATC 9 cut(s) 199, 304, 331, 349, 476, 516, 783, 976, 1069
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 9 cut(s) 196, 301, 328, 346, 473, 513, 780, 973, 1066
BstNSI RCATGY 1 cut(s) 835
BstSCI CCNGG 2 cut(s) 492, 643
BstSFI CTRYAG 1 cut(s) 171
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 6 cut(s) 120, 157, 185, 424, 466, 680
BstXI CCANNNNNNTGG 2 cut(s) 526, 667
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 2 cut(s) 408, 824
BtgI CCRYGG 1 cut(s) 274
BtsCI GGATG 2 cut(s) 72, 172
BtsIMutI CAGTG 1 cut(s) 413
Cac8I GCNNGC 4 cut(s) 246, 267, 271, 877
CaiI CAGNNNCTG 1 cut(s) 133
CciI TCATGA 1 cut(s) 976
Cfr10I RCCGGY 1 cut(s) 680
Cfr13I GGNCC 1 cut(s) 683
CseI GACGC 1 cut(s) 16
Csp6I GTAC 4 cut(s) 294, 395, 764, 865
CspAI ACCGGT 1 cut(s) 680
CspCI CAANNNNNGTGG 2 cut(s) 885, 920
CviAII CATG 6 cut(s) 152, 177, 324, 832, 871, 977
CviQI GTAC 4 cut(s) 294, 395, 764, 865
DdeI CTNAG 7 cut(s) 126, 217, 290, 458, 470, 488, 968
DpnI GATC 9 cut(s) 198, 303, 330, 348, 475, 515, 782, 975, 1068
DpnII GATC 9 cut(s) 196, 301, 328, 346, 473, 513, 780, 973, 1066
EaeI YGGCCR 2 cut(s) 406, 822
Eco47I GGWCC 1 cut(s) 683
Eco88I CYCGRG 1 cut(s) 255
EcoNI CCTNNNNNAGG 1 cut(s) 597
FaeI CATG 6 cut(s) 155, 180, 327, 835, 874, 980
FatI CATG 6 cut(s) 151, 176, 323, 831, 870, 976
FauNDI CATATG 1 cut(s) 1023
FbaI TGATCA 2 cut(s) 780, 973
FblI GTMKAC 1 cut(s) 1062
Fnu4HI GCNGC 8 cut(s) 134, 171, 174, 438, 480, 672, 694, 1091
FokI GGATG 2 cut(s) 59, 179
Fsp4HI GCNGC 8 cut(s) 134, 171, 174, 438, 480, 672, 694, 1091
FspBI CTAG 2 cut(s) 30, 270
GluI GCNGC 8 cut(s) 134, 171, 174, 438, 480, 672, 694, 1091
GsuI CTGGAG 1 cut(s) 177
HaeIII GGCC 2 cut(s) 408, 824
HapII CCGG 5 cut(s) 494, 644, 681, 821, 860
HgaI GACGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 936
Hin1II CATG 6 cut(s) 155, 180, 327, 835, 874, 980
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 201, 989
HinfI GANTC 2 cut(s) 91, 564
HpaII CCGG 5 cut(s) 494, 644, 681, 821, 860
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 8 cut(s) 6, 278, 373, 397, 652, 744, 865, 1063
Hpy188I TCNGA 8 cut(s) 15, 80, 129, 306, 513, 775, 885, 934
Hpy188III TCNNGA 5 cut(s) 30, 99, 194, 784, 977
Hpy8I GTNNAC 8 cut(s) 6, 278, 373, 397, 652, 744, 865, 1063
HpyAV CCTTC 1 cut(s) 943
HpyCH4III ACNGT 1 cut(s) 741
HpyCH4IV ACGT 3 cut(s) 103, 936, 1005
HpyCH4V TGCA 5 cut(s) 85, 173, 696, 835, 926
HpyF3I CTNAG 7 cut(s) 126, 217, 290, 458, 470, 488, 968
HpySE526I ACGT 3 cut(s) 103, 936, 1005
Hsp92I GRCGYC 1 cut(s) 936
Hsp92II CATG 6 cut(s) 155, 180, 327, 835, 874, 980
Ksp22I TGATCA 2 cut(s) 780, 973
Kzo9I GATC 9 cut(s) 196, 301, 328, 346, 473, 513, 780, 973, 1066
LmnI GCTCC 1 cut(s) 662
Lsp1109I GCAGC 6 cut(s) 120, 157, 185, 424, 466, 680
LweI GCATC 2 cut(s) 157, 913
MaeI CTAG 2 cut(s) 30, 270
MaeII ACGT 3 cut(s) 103, 936, 1005
MaeIII GTNAC 6 cut(s) 52, 68, 110, 444, 461, 676
MalI GATC 9 cut(s) 198, 303, 330, 348, 475, 515, 782, 975, 1068
MboI GATC 9 cut(s) 196, 301, 328, 346, 473, 513, 780, 973, 1066
MboII GAAGA 3 cut(s) 74, 341, 1075
MfeI CAATTG 1 cut(s) 332
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 7 cut(s) 115, 332, 364, 534, 661, 688, 814
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 1 cut(s) 558
MmeI TCCRAC 2 cut(s) 329, 728
Mox20I TGGCCA 1 cut(s) 408
MroXI GAANNNNTTC 1 cut(s) 535
MscI TGGCCA 1 cut(s) 408
MseI TTAA 2 cut(s) 828, 960
MslI CAYNNNNRTG 2 cut(s) 521, 830
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 5 cut(s) 494, 644, 681, 821, 860
MspR9I CCNGG 2 cut(s) 494, 645
MunI CAATTG 1 cut(s) 332
Mva1269I GAATGC 2 cut(s) 879, 1051
MvnI CGCG 1 cut(s) 585
NciI CCSGG 2 cut(s) 494, 645
NdeI CATATG 1 cut(s) 1023
NdeII GATC 9 cut(s) 196, 301, 328, 346, 473, 513, 780, 973, 1066
NheI GCTAGC 1 cut(s) 269
NlaIII CATG 6 cut(s) 155, 180, 327, 835, 874, 980
NmuCI GTSAC 1 cut(s) 444
NspI RCATGY 1 cut(s) 835
PaeR7I CTCGAG 1 cut(s) 255
PagI TCATGA 1 cut(s) 976
PctI GAATGC 2 cut(s) 879, 1051
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 1 cut(s) 91
PflMI CCANNNNNTGG 1 cut(s) 415
PinAI ACCGGT 1 cut(s) 680
PkrI GCNGC 8 cut(s) 135, 172, 175, 439, 481, 673, 695, 1092
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
Ppu21I YACGTR 1 cut(s) 1006
PspPI GGNCC 1 cut(s) 683
PspXI VCTCGAGB 1 cut(s) 255
PstI CTGCAG 1 cut(s) 175
PstNI CAGNNNCTG 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 4 cut(s) 295, 396, 765, 866
RsaNI GTAC 4 cut(s) 294, 395, 764, 865
RseI CAYNNNNRTG 2 cut(s) 521, 830
SaqAI TTAA 2 cut(s) 828, 960
SatI GCNGC 8 cut(s) 134, 171, 174, 438, 480, 672, 694, 1091
Sau3AI GATC 9 cut(s) 196, 301, 328, 346, 473, 513, 780, 973, 1066
Sau96I GGNCC 1 cut(s) 683
ScaI AGTACT 1 cut(s) 295
SchI GAGTC 1 cut(s) 558
ScrFI CCNGG 2 cut(s) 494, 645
SfaNI GCATC 2 cut(s) 157, 913
SfcI CTRYAG 1 cut(s) 171
Sfr274I CTCGAG 1 cut(s) 255
SinI GGWCC 1 cut(s) 683
SlaI CTCGAG 1 cut(s) 255
SmiMI CAYNNNNRTG 2 cut(s) 521, 830
SmlI CTYRAG 2 cut(s) 255, 597
SmoI CTYRAG 2 cut(s) 255, 597
Sse9I AATT 7 cut(s) 115, 332, 364, 534, 661, 688, 814
SsiI CCGC 2 cut(s) 583, 671
SspI AATATT 1 cut(s) 45
SspMI CTAG 2 cut(s) 30, 270
StyD4I CCNGG 2 cut(s) 492, 643
TaaI ACNGT 1 cut(s) 741
TaiI ACGT 3 cut(s) 106, 939, 1008
TaqI TCGA 2 cut(s) 119, 256
TasI AATT 7 cut(s) 115, 332, 364, 534, 661, 688, 814
TatI WGTACW 3 cut(s) 293, 394, 864
TauI GCSGC 1 cut(s) 674
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 2 cut(s) 828, 960
Tru9I TTAA 2 cut(s) 828, 960
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 1 cut(s) 444
TseI GCWGC 7 cut(s) 133, 170, 173, 437, 479, 693, 1090
Tsp45I GTSAC 1 cut(s) 444
TspDTI ATGAA 1 cut(s) 1021
TspRI CASTG 1 cut(s) 420
Van91I CCANNNNNTGG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 683
XagI CCTNNNNNAGG 1 cut(s) 597
XapI RAATTY 1 cut(s) 534
XbaI TCTAGA 1 cut(s) 29
XceI RCATGY 1 cut(s) 835
XcmI CCANNNNNNNNNTGG 1 cut(s) 757
XhoI CTCGAG 1 cut(s) 255
XmiI GTMKAC 1 cut(s) 1062
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 2 cut(s) 30, 270
ZraI GACGTC 1 cut(s) 937
ZrmI AGTACT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.