E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 582 |
| AccI | GTMKAC | 1 cut(s) | 669 |
| AccII | CGCG | 1 cut(s) | 327 |
| AciI | CCGC | 2 cut(s) | 458, 580 |
| AclWI | GGATC | 1 cut(s) | 554 |
| AcsI | RAATTY | 1 cut(s) | 102 |
| AcuI | CTGAAG | 1 cut(s) | 596 |
| AfaI | GTAC | 1 cut(s) | 617 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 48 |
| AgsI | TTSAA | 5 cut(s) | 218, 232, 314, 386, 419 |
| AluBI | AGCT | 4 cut(s) | 110, 252, 524, 648 |
| AluI | AGCT | 4 cut(s) | 110, 252, 524, 648 |
| Alw26I | GTCTC | 1 cut(s) | 110 |
| AlwI | GGATC | 1 cut(s) | 554 |
| ApoI | RAATTY | 1 cut(s) | 102 |
| AspLEI | GCGC | 1 cut(s) | 329 |
| AsuHPI | GGTGA | 3 cut(s) | 442, 499, 622 |
| BanI | GGYRCC | 1 cut(s) | 582 |
| BbsI | GAAGAC | 1 cut(s) | 99 |
| BccI | CCATC | 3 cut(s) | 365, 533, 595 |
| BclI | TGATCA | 1 cut(s) | 198 |
| BcoDI | GTCTC | 1 cut(s) | 110 |
| BfaI | CTAG | 5 cut(s) | 107, 111, 305, 455, 679 |
| BisI | GCNGC | 1 cut(s) | 581 |
| BlsI | GCNGC | 1 cut(s) | 582 |
| BmiI | GGNNCC | 2 cut(s) | 569, 584 |
| BpiI | GAAGAC | 1 cut(s) | 99 |
| BpuEI | CTTGAG | 1 cut(s) | 383 |
| Bsa29I | ATCGAT | 1 cut(s) | 549 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 48 |
| BseCI | ATCGAT | 1 cut(s) | 549 |
| BseGI | GGATG | 2 cut(s) | 343, 587 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 48 |
| BseMII | CTCAG | 1 cut(s) | 38 |
| BseRI | GAGGAG | 2 cut(s) | 224, 580 |
| BsgI | GTGCAG | 1 cut(s) | 198 |
| Bsh1236I | CGCG | 1 cut(s) | 327 |
| BshNI | GGYRCC | 1 cut(s) | 582 |
| BshVI | ATCGAT | 1 cut(s) | 549 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 48 |
| BsmAI | GTCTC | 1 cut(s) | 110 |
| Bsp143I | GATC | 6 cut(s) | 58, 168, 198, 262, 394, 546 |
| BspACI | CCGC | 2 cut(s) | 458, 580 |
| BspCNI | CTCAG | 1 cut(s) | 39 |
| BspDI | ATCGAT | 1 cut(s) | 549 |
| BspFNI | CGCG | 1 cut(s) | 327 |
| BspLI | GGNNCC | 2 cut(s) | 569, 584 |
| BspPI | GGATC | 1 cut(s) | 554 |
| BspT107I | GGYRCC | 1 cut(s) | 582 |
| BssMI | GATC | 6 cut(s) | 58, 168, 198, 262, 394, 546 |
| Bst4CI | ACNGT | 1 cut(s) | 121 |
| Bst6I | CTCTTC | 3 cut(s) | 309, 552, 615 |
| BstC8I | GCNNGC | 1 cut(s) | 325 |
| BstDEI | CTNAG | 1 cut(s) | 47 |
| BstF5I | GGATG | 2 cut(s) | 343, 587 |
| BstFNI | CGCG | 1 cut(s) | 327 |
| BstHHI | GCGC | 1 cut(s) | 329 |
| BstKTI | GATC | 6 cut(s) | 61, 171, 201, 265, 397, 549 |
| BstMAI | GTCTC | 1 cut(s) | 110 |
| BstMBI | GATC | 6 cut(s) | 58, 168, 198, 262, 394, 546 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 455 |
| BstUI | CGCG | 1 cut(s) | 327 |
| BstV2I | GAAGAC | 1 cut(s) | 99 |
| Bsu15I | ATCGAT | 1 cut(s) | 549 |
| BsuTUI | ATCGAT | 1 cut(s) | 549 |
| BtsCI | GGATG | 2 cut(s) | 343, 587 |
| BtsI | GCAGTG | 3 cut(s) | 14, 181, 186 |
| BtsIMutI | CAGTG | 4 cut(s) | 14, 126, 181, 186 |
| Cac8I | GCNNGC | 1 cut(s) | 325 |
| CfoI | GCGC | 1 cut(s) | 329 |
| ClaI | ATCGAT | 1 cut(s) | 549 |
| Csp6I | GTAC | 1 cut(s) | 616 |
| CviAII | CATG | 3 cut(s) | 469, 536, 604 |
| CviJI | RGCY | 7 cut(s) | 110, 252, 411, 449, 524, 570, 648 |
| CviKI_1 | RGCY | 7 cut(s) | 110, 252, 411, 449, 524, 570, 648 |
| CviQI | GTAC | 1 cut(s) | 616 |
| DdeI | CTNAG | 1 cut(s) | 47 |
| DpnI | GATC | 6 cut(s) | 60, 170, 200, 264, 396, 548 |
| DpnII | GATC | 6 cut(s) | 58, 168, 198, 262, 394, 546 |
| Eam1104I | CTCTTC | 3 cut(s) | 309, 552, 615 |
| EarI | CTCTTC | 3 cut(s) | 309, 552, 615 |
| Eco57I | CTGAAG | 1 cut(s) | 596 |
| FaeI | CATG | 3 cut(s) | 472, 539, 607 |
| FaiI | YATR | 9 cut(s) | 14, 34, 72, 153, 391, 470, 500, 537, 605 |
| FatI | CATG | 3 cut(s) | 468, 535, 603 |
| FbaI | TGATCA | 1 cut(s) | 198 |
| FblI | GTMKAC | 1 cut(s) | 669 |
| Fnu4HI | GCNGC | 1 cut(s) | 581 |
| FokI | GGATG | 2 cut(s) | 330, 574 |
| Fsp4HI | GCNGC | 1 cut(s) | 581 |
| FspBI | CTAG | 5 cut(s) | 107, 111, 305, 455, 679 |
| GlaI | GCGC | 1 cut(s) | 328 |
| GluI | GCNGC | 1 cut(s) | 581 |
| HhaI | GCGC | 1 cut(s) | 329 |
| Hin1II | CATG | 3 cut(s) | 472, 539, 607 |
| Hin6I | GCGC | 1 cut(s) | 327 |
| HinP1I | GCGC | 1 cut(s) | 327 |
| HincII | GTYRAC | 1 cut(s) | 670 |
| HindII | GTYRAC | 1 cut(s) | 670 |
| HindIII | AAGCTT | 1 cut(s) | 646 |
| HinfI | GANTC | 3 cut(s) | 461, 472, 607 |
| HphI | GGTGA | 3 cut(s) | 442, 499, 622 |
| Hpy166II | GTNNAC | 1 cut(s) | 670 |
| Hpy188I | TCNGA | 1 cut(s) | 481 |
| Hpy188III | TCNNGA | 3 cut(s) | 62, 81, 232 |
| Hpy8I | GTNNAC | 1 cut(s) | 670 |
| Hpy99I | CGWCG | 2 cut(s) | 365, 674 |
| HpyAV | CCTTC | 1 cut(s) | 602 |
| HpyCH4III | ACNGT | 1 cut(s) | 121 |
| HpyCH4V | TGCA | 3 cut(s) | 7, 12, 179 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 455 |
| HpyF3I | CTNAG | 1 cut(s) | 47 |
| Hsp92II | CATG | 3 cut(s) | 472, 539, 607 |
| HspAI | GCGC | 1 cut(s) | 327 |
| Ksp22I | TGATCA | 1 cut(s) | 198 |
| Kzo9I | GATC | 6 cut(s) | 58, 168, 198, 262, 394, 546 |
| LmnI | GCTCC | 1 cut(s) | 567 |
| LpnPI | CCDG | 3 cut(s) | 111, 410, 450 |
| MaeI | CTAG | 5 cut(s) | 107, 111, 305, 455, 679 |
| MalI | GATC | 6 cut(s) | 60, 170, 200, 264, 396, 548 |
| MboI | GATC | 6 cut(s) | 58, 168, 198, 262, 394, 546 |
| MboII | GAAGA | 5 cut(s) | 104, 178, 326, 569, 602 |
| MfeI | CAATTG | 1 cut(s) | 218 |
| MluCI | AATT | 4 cut(s) | 102, 218, 386, 501 |
| MlyI | GAGTC | 1 cut(s) | 601 |
| MseI | TTAA | 3 cut(s) | 23, 162, 597 |
| MslI | CAYNNNNRTG | 1 cut(s) | 33 |
| MunI | CAATTG | 1 cut(s) | 218 |
| MvnI | CGCG | 1 cut(s) | 327 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 455 |
| NdeII | GATC | 6 cut(s) | 58, 168, 198, 262, 394, 546 |
| NlaIII | CATG | 3 cut(s) | 472, 539, 607 |
| NlaIV | GGNNCC | 2 cut(s) | 569, 584 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 357 |
| PfeI | GAWTC | 2 cut(s) | 461, 472 |
| PkrI | GCNGC | 1 cut(s) | 582 |
| PleI | GAGTC | 1 cut(s) | 601 |
| PpsI | GAGTC | 1 cut(s) | 601 |
| PspN4I | GGNNCC | 2 cut(s) | 569, 584 |
| RsaI | GTAC | 1 cut(s) | 617 |
| RsaNI | GTAC | 1 cut(s) | 616 |
| RseI | CAYNNNNRTG | 1 cut(s) | 33 |
| SalI | GTCGAC | 1 cut(s) | 668 |
| SaqAI | TTAA | 3 cut(s) | 23, 162, 597 |
| SatI | GCNGC | 1 cut(s) | 581 |
| Sau3AI | GATC | 6 cut(s) | 58, 168, 198, 262, 394, 546 |
| SchI | GAGTC | 1 cut(s) | 601 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 33 |
| SmlI | CTYRAG | 1 cut(s) | 398 |
| SmoI | CTYRAG | 1 cut(s) | 398 |
| Sse9I | AATT | 4 cut(s) | 102, 218, 386, 501 |
| SsiI | CCGC | 2 cut(s) | 458, 580 |
| SspMI | CTAG | 5 cut(s) | 107, 111, 305, 455, 679 |
| TaaI | ACNGT | 1 cut(s) | 121 |
| TaqI | TCGA | 4 cut(s) | 80, 360, 549, 669 |
| TasI | AATT | 4 cut(s) | 102, 218, 386, 501 |
| TauI | GCSGC | 1 cut(s) | 583 |
| TfiI | GAWTC | 2 cut(s) | 461, 472 |
| Tru1I | TTAA | 3 cut(s) | 23, 162, 597 |
| Tru9I | TTAA | 3 cut(s) | 23, 162, 597 |
| TscAI | CASTG | 4 cut(s) | 14, 126, 181, 186 |
| TspDTI | ATGAA | 3 cut(s) | 332, 403, 485 |
| TspGWI | ACGGA | 1 cut(s) | 207 |
| TspRI | CASTG | 4 cut(s) | 14, 126, 181, 186 |
| XapI | RAATTY | 1 cut(s) | 102 |
| XmiI | GTMKAC | 1 cut(s) | 669 |
| XspI | CTAG | 5 cut(s) | 107, 111, 305, 455, 679 |