E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 886 |
| AciI | CCGC | 1 cut(s) | 132 |
| AcoI | YGGCCR | 2 cut(s) | 129, 585 |
| AcsI | RAATTY | 2 cut(s) | 139, 860 |
| AcuI | CTGAAG | 2 cut(s) | 210, 592 |
| AfaI | GTAC | 4 cut(s) | 454, 479, 733, 883 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 563 |
| AflIII | ACRYGT | 1 cut(s) | 530 |
| AgsI | TTSAA | 4 cut(s) | 170, 797, 820, 915 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 29 |
| AjnI | CCWGG | 2 cut(s) | 80, 271 |
| AluBI | AGCT | 6 cut(s) | 62, 230, 350, 702, 789, 804 |
| AluI | AGCT | 6 cut(s) | 62, 230, 350, 702, 789, 804 |
| Alw21I | GWGCWC | 1 cut(s) | 207 |
| Alw26I | GTCTC | 1 cut(s) | 873 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 871 |
| AoxI | GGCC | 3 cut(s) | 43, 129, 585 |
| ApeKI | GCWGC | 2 cut(s) | 363, 483 |
| ApoI | RAATTY | 2 cut(s) | 139, 860 |
| AspLEI | GCGC | 1 cut(s) | 990 |
| AspS9I | GGNCC | 1 cut(s) | 44 |
| AsuHPI | GGTGA | 2 cut(s) | 508, 534 |
| AsuNHI | GCTAGC | 1 cut(s) | 230 |
| BbsI | GAAGAC | 1 cut(s) | 313 |
| Bbv12I | GWGCWC | 1 cut(s) | 207 |
| BbvI | GCAGC | 2 cut(s) | 350, 470 |
| BccI | CCATC | 2 cut(s) | 196, 437 |
| BceAI | ACGGC | 2 cut(s) | 58, 496 |
| BciT130I | CCWGG | 2 cut(s) | 82, 273 |
| BclI | TGATCA | 2 cut(s) | 331, 616 |
| BcoDI | GTCTC | 1 cut(s) | 873 |
| BfaI | CTAG | 3 cut(s) | 231, 347, 801 |
| BfmI | CTRYAG | 1 cut(s) | 893 |
| BfuAI | ACCTGC | 1 cut(s) | 886 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 408 |
| BisI | GCNGC | 3 cut(s) | 132, 364, 484 |
| BlsI | GCNGC | 3 cut(s) | 133, 365, 485 |
| BmcAI | AGTACT | 1 cut(s) | 883 |
| Bme1390I | CCNGG | 2 cut(s) | 82, 273 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 29 |
| BmgT120I | GGNCC | 1 cut(s) | 44 |
| BmiI | GGNNCC | 1 cut(s) | 412 |
| BmrFI | CCNGG | 2 cut(s) | 82, 273 |
| BmsI | GCATC | 3 cut(s) | 76, 352, 557 |
| BmtI | GCTAGC | 1 cut(s) | 234 |
| BpiI | GAAGAC | 1 cut(s) | 313 |
| BpmI | CTGGAG | 2 cut(s) | 42, 219 |
| BsaJI | CCNNGG | 5 cut(s) | 40, 81, 271, 582, 588 |
| BsaWI | WCCGGW | 2 cut(s) | 12, 493 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 563 |
| Bse1I | ACTGG | 1 cut(s) | 325 |
| BseBI | CCWGG | 2 cut(s) | 82, 273 |
| BseDI | CCNNGG | 5 cut(s) | 40, 81, 271, 582, 588 |
| BseGI | GGATG | 3 cut(s) | 18, 91, 572 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 563 |
| BseMII | CTCAG | 1 cut(s) | 104 |
| BseNI | ACTGG | 1 cut(s) | 325 |
| BseXI | GCAGC | 2 cut(s) | 350, 470 |
| BsgI | GTGCAG | 2 cut(s) | 90, 349 |
| BshFI | GGCC | 3 cut(s) | 45, 131, 587 |
| BsiHKAI | GWGCWC | 1 cut(s) | 207 |
| BsiSI | CCGG | 2 cut(s) | 13, 494 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 563 |
| BsmAI | GTCTC | 1 cut(s) | 873 |
| BsnI | GGCC | 3 cut(s) | 45, 131, 587 |
| Bsp1286I | GDGCHC | 1 cut(s) | 207 |
| Bsp143I | GATC | 4 cut(s) | 6, 331, 616, 826 |
| BspACI | CCGC | 1 cut(s) | 132 |
| BspANI | GGCC | 3 cut(s) | 45, 131, 587 |
| BspCNI | CTCAG | 1 cut(s) | 103 |
| BspLI | GGNNCC | 1 cut(s) | 412 |
| BspMAI | CTGCAG | 1 cut(s) | 897 |
| BspMI | ACCTGC | 1 cut(s) | 886 |
| BspOI | GCTAGC | 1 cut(s) | 234 |
| BsrI | ACTGG | 1 cut(s) | 325 |
| BssECI | CCNNGG | 5 cut(s) | 40, 81, 271, 582, 588 |
| BssMI | GATC | 4 cut(s) | 6, 331, 616, 826 |
| BssT1I | CCWWGG | 1 cut(s) | 588 |
| Bst2UI | CCWGG | 2 cut(s) | 82, 273 |
| Bst4CI | ACNGT | 3 cut(s) | 313, 671, 784 |
| BstC8I | GCNNGC | 3 cut(s) | 232, 352, 893 |
| BstDEI | CTNAG | 1 cut(s) | 90 |
| BstDSI | CCRYGG | 1 cut(s) | 40 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 561 |
| BstF5I | GGATG | 3 cut(s) | 18, 91, 572 |
| BstHHI | GCGC | 1 cut(s) | 990 |
| BstKTI | GATC | 4 cut(s) | 9, 334, 619, 829 |
| BstMAI | GTCTC | 1 cut(s) | 873 |
| BstMBI | GATC | 4 cut(s) | 6, 331, 616, 826 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 68, 240, 294, 360, 408 |
| BstNI | CCWGG | 2 cut(s) | 82, 273 |
| BstNSI | RCATGY | 1 cut(s) | 534 |
| BstSCI | CCNGG | 2 cut(s) | 80, 271 |
| BstSFI | CTRYAG | 1 cut(s) | 893 |
| BstV1I | GCAGC | 2 cut(s) | 350, 470 |
| BstV2I | GAAGAC | 1 cut(s) | 313 |
| BsuRI | GGCC | 3 cut(s) | 45, 131, 587 |
| BtgI | CCRYGG | 1 cut(s) | 40 |
| BtsCI | GGATG | 3 cut(s) | 18, 91, 572 |
| BtsIMutI | CAGTG | 1 cut(s) | 332 |
| BveI | ACCTGC | 1 cut(s) | 886 |
| Cac8I | GCNNGC | 3 cut(s) | 232, 352, 893 |
| CaiI | CAGNNNCTG | 1 cut(s) | 871 |
| CfoI | GCGC | 1 cut(s) | 990 |
| Cfr13I | GGNCC | 1 cut(s) | 44 |
| Csp6I | GTAC | 4 cut(s) | 453, 478, 732, 882 |
| CviAII | CATG | 5 cut(s) | 521, 531, 552, 808, 936 |
| CviQI | GTAC | 4 cut(s) | 453, 478, 732, 882 |
| DdeI | CTNAG | 1 cut(s) | 90 |
| DpnI | GATC | 4 cut(s) | 8, 333, 618, 828 |
| DpnII | GATC | 4 cut(s) | 6, 331, 616, 826 |
| DriI | GACNNNNNGTC | 1 cut(s) | 29 |
| EaeI | YGGCCR | 2 cut(s) | 129, 585 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 29 |
| Eco130I | CCWWGG | 1 cut(s) | 588 |
| Eco57I | CTGAAG | 2 cut(s) | 210, 592 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 561 |
| EcoRII | CCWGG | 2 cut(s) | 80, 271 |
| EcoT14I | CCWWGG | 1 cut(s) | 588 |
| ErhI | CCWWGG | 1 cut(s) | 588 |
| FaeI | CATG | 5 cut(s) | 524, 534, 555, 811, 939 |
| FatI | CATG | 5 cut(s) | 520, 530, 551, 807, 935 |
| FauNDI | CATATG | 1 cut(s) | 992 |
| FbaI | TGATCA | 2 cut(s) | 331, 616 |
| Fnu4HI | GCNGC | 3 cut(s) | 132, 364, 484 |
| FokI | GGATG | 3 cut(s) | 5, 98, 579 |
| Fsp4HI | GCNGC | 3 cut(s) | 132, 364, 484 |
| FspBI | CTAG | 3 cut(s) | 231, 347, 801 |
| GlaI | GCGC | 1 cut(s) | 989 |
| GluI | GCNGC | 3 cut(s) | 132, 364, 484 |
| GsuI | CTGGAG | 2 cut(s) | 42, 219 |
| HaeIII | GGCC | 3 cut(s) | 45, 131, 587 |
| HapII | CCGG | 2 cut(s) | 13, 494 |
| HhaI | GCGC | 1 cut(s) | 990 |
| Hin1II | CATG | 5 cut(s) | 524, 534, 555, 811, 939 |
| Hin6I | GCGC | 1 cut(s) | 988 |
| HinP1I | GCGC | 1 cut(s) | 988 |
| HinfI | GANTC | 4 cut(s) | 50, 446, 626, 950 |
| HpaII | CCGG | 2 cut(s) | 13, 494 |
| HphI | GGTGA | 2 cut(s) | 508, 534 |
| Hpy166II | GTNNAC | 2 cut(s) | 302, 542 |
| Hpy188I | TCNGA | 4 cut(s) | 23, 93, 124, 873 |
| Hpy188III | TCNNGA | 5 cut(s) | 147, 433, 716, 915, 967 |
| Hpy8I | GTNNAC | 2 cut(s) | 302, 542 |
| HpyAV | CCTTC | 2 cut(s) | 233, 921 |
| HpyCH4III | ACNGT | 3 cut(s) | 313, 671, 784 |
| HpyCH4V | TGCA | 6 cut(s) | 71, 366, 465, 486, 570, 895 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 68, 240, 294, 360, 408 |
| HpyF3I | CTNAG | 1 cut(s) | 90 |
| Hsp92II | CATG | 5 cut(s) | 524, 534, 555, 811, 939 |
| HspAI | GCGC | 1 cut(s) | 988 |
| Ksp22I | TGATCA | 2 cut(s) | 331, 616 |
| Kzo9I | GATC | 4 cut(s) | 6, 331, 616, 826 |
| LmnI | GCTCC | 2 cut(s) | 359, 416 |
| Lsp1109I | GCAGC | 2 cut(s) | 350, 470 |
| LweI | GCATC | 3 cut(s) | 76, 352, 557 |
| MaeI | CTAG | 3 cut(s) | 231, 347, 801 |
| MaeIII | GTNAC | 2 cut(s) | 496, 778 |
| MalI | GATC | 4 cut(s) | 8, 333, 618, 828 |
| MboI | GATC | 4 cut(s) | 6, 331, 616, 826 |
| MboII | GAAGA | 4 cut(s) | 313, 448, 523, 821 |
| MhlI | GDGCHC | 1 cut(s) | 207 |
| MluCI | AATT | 4 cut(s) | 139, 291, 678, 860 |
| MlyI | GAGTC | 1 cut(s) | 944 |
| MmeI | TCCRAC | 1 cut(s) | 46 |
| MnlI | CCTC | 5 cut(s) | 37, 97, 592, 755, 805 |
| MseI | TTAA | 3 cut(s) | 263, 900, 929 |
| MslI | CAYNNNNRTG | 1 cut(s) | 535 |
| MspA1I | CMGCKG | 2 cut(s) | 62, 134 |
| MspI | CCGG | 2 cut(s) | 13, 494 |
| MspR9I | CCNGG | 2 cut(s) | 82, 273 |
| MvaI | CCWGG | 2 cut(s) | 82, 273 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 68, 240, 294, 360, 408 |
| NdeI | CATATG | 1 cut(s) | 992 |
| NdeII | GATC | 4 cut(s) | 6, 331, 616, 826 |
| NheI | GCTAGC | 1 cut(s) | 230 |
| NlaIII | CATG | 5 cut(s) | 524, 534, 555, 811, 939 |
| NlaIV | GGNNCC | 1 cut(s) | 412 |
| NmeAIII | GCCGAG | 2 cut(s) | 449, 563 |
| NmuCI | GTSAC | 2 cut(s) | 496, 778 |
| NspI | RCATGY | 1 cut(s) | 534 |
| PciI | ACATGT | 1 cut(s) | 530 |
| PfeI | GAWTC | 3 cut(s) | 50, 446, 626 |
| PkrI | GCNGC | 3 cut(s) | 133, 365, 485 |
| PleI | GAGTC | 1 cut(s) | 944 |
| PpsI | GAGTC | 1 cut(s) | 944 |
| PscI | ACATGT | 1 cut(s) | 530 |
| Psp6I | CCWGG | 2 cut(s) | 80, 271 |
| PspGI | CCWGG | 2 cut(s) | 80, 271 |
| PspN4I | GGNNCC | 1 cut(s) | 412 |
| PspPI | GGNCC | 1 cut(s) | 44 |
| PstI | CTGCAG | 1 cut(s) | 897 |
| PstNI | CAGNNNCTG | 1 cut(s) | 871 |
| PvuII | CAGCTG | 1 cut(s) | 62 |
| RsaI | GTAC | 4 cut(s) | 454, 479, 733, 883 |
| RsaNI | GTAC | 4 cut(s) | 453, 478, 732, 882 |
| RseI | CAYNNNNRTG | 1 cut(s) | 535 |
| SaqAI | TTAA | 3 cut(s) | 263, 900, 929 |
| SatI | GCNGC | 3 cut(s) | 132, 364, 484 |
| Sau3AI | GATC | 4 cut(s) | 6, 331, 616, 826 |
| Sau96I | GGNCC | 1 cut(s) | 44 |
| SbfI | CCTGCAGG | 1 cut(s) | 897 |
| ScaI | AGTACT | 1 cut(s) | 883 |
| SchI | GAGTC | 1 cut(s) | 944 |
| ScrFI | CCNGG | 2 cut(s) | 82, 273 |
| SdaI | CCTGCAGG | 1 cut(s) | 897 |
| SduI | GDGCHC | 1 cut(s) | 207 |
| SfaNI | GCATC | 3 cut(s) | 76, 352, 557 |
| SfcI | CTRYAG | 1 cut(s) | 893 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 535 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 897 |
| Sse9I | AATT | 4 cut(s) | 139, 291, 678, 860 |
| SsiI | CCGC | 1 cut(s) | 132 |
| SspI | AATATT | 2 cut(s) | 250, 490 |
| SspMI | CTAG | 3 cut(s) | 231, 347, 801 |
| StyD4I | CCNGG | 2 cut(s) | 80, 271 |
| StyI | CCWWGG | 1 cut(s) | 588 |
| TaaI | ACNGT | 3 cut(s) | 313, 671, 784 |
| TaqI | TCGA | 5 cut(s) | 5, 221, 432, 715, 948 |
| TasI | AATT | 4 cut(s) | 139, 291, 678, 860 |
| TatI | WGTACW | 2 cut(s) | 452, 881 |
| TauI | GCSGC | 1 cut(s) | 134 |
| TfiI | GAWTC | 3 cut(s) | 50, 446, 626 |
| Tru1I | TTAA | 3 cut(s) | 263, 900, 929 |
| Tru9I | TTAA | 3 cut(s) | 263, 900, 929 |
| TscAI | CASTG | 1 cut(s) | 332 |
| TseFI | GTSAC | 2 cut(s) | 496, 778 |
| TseI | GCWGC | 2 cut(s) | 363, 483 |
| Tsp45I | GTSAC | 2 cut(s) | 496, 778 |
| TspDTI | ATGAA | 4 cut(s) | 531, 537, 540, 796 |
| TspRI | CASTG | 1 cut(s) | 332 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 561 |
| XapI | RAATTY | 2 cut(s) | 139, 860 |
| XceI | RCATGY | 1 cut(s) | 534 |
| XspI | CTAG | 3 cut(s) | 231, 347, 801 |
| ZrmI | AGTACT | 1 cut(s) | 883 |