E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 16 |
| AciI | CCGC | 1 cut(s) | 73 |
| AclWI | GGATC | 3 cut(s) | 51, 64, 658 |
| AcoI | YGGCCR | 1 cut(s) | 939 |
| AcsI | RAATTY | 1 cut(s) | 602 |
| AcuI | CTGAAG | 1 cut(s) | 782 |
| AdeI | CACNNNGTG | 1 cut(s) | 70 |
| AfaI | GTAC | 4 cut(s) | 32, 484, 509, 862 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 593 |
| AgsI | TTSAA | 6 cut(s) | 335, 703, 731, 815, 829, 936 |
| AjnI | CCWGG | 1 cut(s) | 471 |
| AluBI | AGCT | 6 cut(s) | 311, 383, 399, 675, 822, 988 |
| AluI | AGCT | 6 cut(s) | 311, 383, 399, 675, 822, 988 |
| Alw26I | GTCTC | 1 cut(s) | 1014 |
| AlwI | GGATC | 3 cut(s) | 51, 64, 658 |
| AoxI | GGCC | 1 cut(s) | 939 |
| ApeKI | GCWGC | 4 cut(s) | 387, 396, 513, 597 |
| ApoI | RAATTY | 1 cut(s) | 602 |
| AspS9I | GGNCC | 1 cut(s) | 469 |
| AsuHPI | GGTGA | 3 cut(s) | 16, 224, 283 |
| AsuII | TTCGAA | 1 cut(s) | 639 |
| AvaII | GGWCC | 1 cut(s) | 469 |
| BaeGI | GKGCMC | 1 cut(s) | 378 |
| BalI | TGGCCA | 1 cut(s) | 941 |
| BamHI | GGATCC | 1 cut(s) | 56 |
| BauI | CACGAG | 1 cut(s) | 306 |
| BbsI | GAAGAC | 1 cut(s) | 803 |
| BbvCI | CCTCAGC | 1 cut(s) | 989 |
| BbvI | GCAGC | 4 cut(s) | 374, 408, 500, 584 |
| BccI | CCATC | 3 cut(s) | 353, 427, 917 |
| BceAI | ACGGC | 1 cut(s) | 526 |
| BciT130I | CCWGG | 1 cut(s) | 473 |
| BclI | TGATCA | 1 cut(s) | 982 |
| BcoDI | GTCTC | 1 cut(s) | 1014 |
| BfaI | CTAG | 5 cut(s) | 149, 161, 444, 819, 858 |
| BfmI | CTRYAG | 1 cut(s) | 394 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 438 |
| BisI | GCNGC | 4 cut(s) | 388, 397, 514, 598 |
| BlsI | GCNGC | 4 cut(s) | 389, 398, 515, 599 |
| Bme1390I | CCNGG | 1 cut(s) | 473 |
| Bme18I | GGWCC | 1 cut(s) | 469 |
| BmgT120I | GGNCC | 1 cut(s) | 469 |
| BmiI | GGNNCC | 1 cut(s) | 58 |
| BmrFI | CCNGG | 1 cut(s) | 473 |
| BmrI | ACTGGG | 2 cut(s) | 344, 953 |
| BmuI | ACTGGG | 2 cut(s) | 344, 953 |
| BpiI | GAAGAC | 1 cut(s) | 803 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 861, 893 |
| Bpu10I | CCTNAGC | 1 cut(s) | 989 |
| Bpu14I | TTCGAA | 1 cut(s) | 639 |
| BsaI | GGTCTC | 1 cut(s) | 1014 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 383, 413 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 593 |
| Bse118I | RCCGGY | 1 cut(s) | 653 |
| Bse1I | ACTGG | 3 cut(s) | 350, 358, 959 |
| Bse3DI | GCAATG | 1 cut(s) | 523 |
| BseBI | CCWGG | 1 cut(s) | 473 |
| BseGI | GGATG | 3 cut(s) | 43, 345, 999 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 593 |
| BseMI | GCAATG | 1 cut(s) | 523 |
| BseMII | CTCAG | 2 cut(s) | 892, 980 |
| BseNI | ACTGG | 3 cut(s) | 350, 358, 959 |
| BseSI | GKGCMC | 1 cut(s) | 378 |
| BseXI | GCAGC | 4 cut(s) | 374, 408, 500, 584 |
| BshFI | GGCC | 1 cut(s) | 941 |
| BsiSI | CCGG | 1 cut(s) | 654 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 593 |
| BsmAI | GTCTC | 1 cut(s) | 1014 |
| BsmI | GAATGC | 1 cut(s) | 796 |
| BsnI | GGCC | 1 cut(s) | 941 |
| Bso31I | GGTCTC | 1 cut(s) | 1014 |
| Bsp119I | TTCGAA | 1 cut(s) | 639 |
| Bsp1286I | GDGCHC | 1 cut(s) | 378 |
| Bsp143I | GATC | 5 cut(s) | 56, 157, 663, 974, 982 |
| BspACI | CCGC | 1 cut(s) | 73 |
| BspANI | GGCC | 1 cut(s) | 941 |
| BspCNI | CTCAG | 2 cut(s) | 891, 981 |
| BspLI | GGNNCC | 1 cut(s) | 58 |
| BspMAI | CTGCAG | 1 cut(s) | 398 |
| BspPI | GGATC | 3 cut(s) | 51, 64, 658 |
| BspT104I | TTCGAA | 1 cut(s) | 639 |
| BspTNI | GGTCTC | 1 cut(s) | 1014 |
| BsrDI | GCAATG | 1 cut(s) | 523 |
| BsrFI | RCCGGY | 1 cut(s) | 653 |
| BsrI | ACTGG | 3 cut(s) | 350, 358, 959 |
| BssAI | RCCGGY | 1 cut(s) | 653 |
| BssMI | GATC | 5 cut(s) | 56, 157, 663, 974, 982 |
| BssSI | CACGAG | 1 cut(s) | 306 |
| Bst2BI | CACGAG | 1 cut(s) | 306 |
| Bst2UI | CCWGG | 1 cut(s) | 473 |
| Bst4CI | ACNGT | 2 cut(s) | 683, 888 |
| Bst6I | CTCTTC | 3 cut(s) | 198, 237, 824 |
| BstBI | TTCGAA | 1 cut(s) | 639 |
| BstC8I | GCNNGC | 3 cut(s) | 385, 562, 566 |
| BstDEI | CTNAG | 3 cut(s) | 123, 878, 989 |
| BstF5I | GGATG | 3 cut(s) | 43, 345, 999 |
| BstKTI | GATC | 5 cut(s) | 59, 160, 666, 977, 985 |
| BstMAI | GTCTC | 1 cut(s) | 1014 |
| BstMBI | GATC | 5 cut(s) | 56, 157, 663, 974, 982 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 327, 393, 396, 438, 510, 659 |
| BstNI | CCWGG | 1 cut(s) | 473 |
| BstNSI | RCATGY | 2 cut(s) | 564, 568 |
| BstSCI | CCNGG | 1 cut(s) | 471 |
| BstSFI | CTRYAG | 1 cut(s) | 394 |
| BstSLI | GKGCMC | 1 cut(s) | 378 |
| BstV1I | GCAGC | 4 cut(s) | 374, 408, 500, 584 |
| BstV2I | GAAGAC | 1 cut(s) | 803 |
| BstX2I | RGATCY | 1 cut(s) | 56 |
| BstYI | RGATCY | 1 cut(s) | 56 |
| BsuRI | GGCC | 1 cut(s) | 941 |
| BtsCI | GGATG | 3 cut(s) | 43, 345, 999 |
| BtsI | GCAGTG | 1 cut(s) | 801 |
| BtsIMutI | CAGTG | 3 cut(s) | 357, 365, 801 |
| Cac8I | GCNNGC | 3 cut(s) | 385, 562, 566 |
| Cfr10I | RCCGGY | 1 cut(s) | 653 |
| Cfr13I | GGNCC | 1 cut(s) | 469 |
| Csp6I | GTAC | 4 cut(s) | 31, 483, 508, 861 |
| CviAII | CATG | 8 cut(s) | 423, 492, 526, 551, 561, 565, 650, 914 |
| CviQI | GTAC | 4 cut(s) | 31, 483, 508, 861 |
| DdeI | CTNAG | 3 cut(s) | 123, 878, 989 |
| DpnI | GATC | 5 cut(s) | 58, 159, 665, 976, 984 |
| DpnII | GATC | 5 cut(s) | 56, 157, 663, 974, 982 |
| DraIII | CACNNNGTG | 1 cut(s) | 70 |
| EaeI | YGGCCR | 1 cut(s) | 939 |
| Eam1104I | CTCTTC | 3 cut(s) | 198, 237, 824 |
| EarI | CTCTTC | 3 cut(s) | 198, 237, 824 |
| Eco31I | GGTCTC | 1 cut(s) | 1014 |
| Eco32I | GATATC | 2 cut(s) | 129, 170 |
| Eco47I | GGWCC | 1 cut(s) | 469 |
| Eco57I | CTGAAG | 1 cut(s) | 782 |
| EcoO109I | RGGNCCY | 1 cut(s) | 469 |
| EcoRII | CCWGG | 1 cut(s) | 471 |
| EcoRV | GATATC | 2 cut(s) | 129, 170 |
| EcoT22I | ATGCAT | 2 cut(s) | 566, 570 |
| FaeI | CATG | 8 cut(s) | 426, 495, 529, 554, 564, 568, 653, 917 |
| FatI | CATG | 8 cut(s) | 422, 491, 525, 550, 560, 564, 649, 913 |
| FauNDI | CATATG | 1 cut(s) | 413 |
| FbaI | TGATCA | 1 cut(s) | 982 |
| FblI | GTMKAC | 1 cut(s) | 16 |
| Fnu4HI | GCNGC | 4 cut(s) | 388, 397, 514, 598 |
| FokI | GGATG | 3 cut(s) | 50, 332, 1006 |
| Fsp4HI | GCNGC | 4 cut(s) | 388, 397, 514, 598 |
| FspBI | CTAG | 5 cut(s) | 149, 161, 444, 819, 858 |
| GluI | GCNGC | 4 cut(s) | 388, 397, 514, 598 |
| HaeIII | GGCC | 1 cut(s) | 941 |
| HapII | CCGG | 1 cut(s) | 654 |
| Hin1II | CATG | 8 cut(s) | 426, 495, 529, 554, 564, 568, 653, 917 |
| HincII | GTYRAC | 1 cut(s) | 17 |
| HindII | GTYRAC | 1 cut(s) | 17 |
| HinfI | GANTC | 6 cut(s) | 81, 476, 669, 766, 836, 997 |
| HpaII | CCGG | 1 cut(s) | 654 |
| HphI | GGTGA | 3 cut(s) | 16, 224, 283 |
| Hpy166II | GTNNAC | 1 cut(s) | 17 |
| Hpy188I | TCNGA | 3 cut(s) | 133, 668, 974 |
| Hpy188III | TCNNGA | 6 cut(s) | 89, 105, 155, 731, 760, 826 |
| Hpy8I | GTNNAC | 1 cut(s) | 17 |
| Hpy99I | CGWCG | 1 cut(s) | 90 |
| HpyAV | CCTTC | 4 cut(s) | 460, 737, 773, 942 |
| HpyCH4III | ACNGT | 2 cut(s) | 683, 888 |
| HpyCH4IV | ACGT | 1 cut(s) | 288 |
| HpyCH4V | TGCA | 8 cut(s) | 53, 200, 390, 396, 516, 564, 568, 794 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 327, 393, 396, 438, 510, 659 |
| HpyF3I | CTNAG | 3 cut(s) | 123, 878, 989 |
| HpySE526I | ACGT | 1 cut(s) | 288 |
| Hsp92II | CATG | 8 cut(s) | 426, 495, 529, 554, 564, 568, 653, 917 |
| Ksp22I | TGATCA | 1 cut(s) | 982 |
| Kzo9I | GATC | 5 cut(s) | 56, 157, 663, 974, 982 |
| LmnI | GCTCC | 1 cut(s) | 404 |
| Lsp1109I | GCAGC | 4 cut(s) | 374, 408, 500, 584 |
| MaeI | CTAG | 5 cut(s) | 149, 161, 444, 819, 858 |
| MaeII | ACGT | 1 cut(s) | 288 |
| MaeIII | GTNAC | 3 cut(s) | 152, 289, 882 |
| MalI | GATC | 5 cut(s) | 58, 159, 665, 976, 984 |
| MboI | GATC | 5 cut(s) | 56, 157, 663, 974, 982 |
| MflI | RGATCY | 1 cut(s) | 56 |
| MhlI | GDGCHC | 1 cut(s) | 378 |
| MlsI | TGGCCA | 1 cut(s) | 941 |
| MluCI | AATT | 4 cut(s) | 256, 324, 486, 602 |
| MluNI | TGGCCA | 1 cut(s) | 941 |
| MlyI | GAGTC | 2 cut(s) | 90, 845 |
| Mox20I | TGGCCA | 1 cut(s) | 941 |
| Mph1103I | ATGCAT | 2 cut(s) | 566, 570 |
| MscI | TGGCCA | 1 cut(s) | 941 |
| MseI | TTAA | 4 cut(s) | 285, 296, 341, 626 |
| MslI | CAYNNNNRTG | 2 cut(s) | 350, 918 |
| Msp20I | TGGCCA | 1 cut(s) | 941 |
| MspA1I | CMGCKG | 1 cut(s) | 988 |
| MspI | CCGG | 1 cut(s) | 654 |
| MspR9I | CCNGG | 1 cut(s) | 473 |
| Mva1269I | GAATGC | 1 cut(s) | 796 |
| MvaI | CCWGG | 1 cut(s) | 473 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 327, 393, 396, 438, 510, 659 |
| NdeI | CATATG | 1 cut(s) | 413 |
| NdeII | GATC | 5 cut(s) | 56, 157, 663, 974, 982 |
| NlaIII | CATG | 8 cut(s) | 426, 495, 529, 554, 564, 568, 653, 917 |
| NlaIV | GGNNCC | 1 cut(s) | 58 |
| NmuCI | GTSAC | 2 cut(s) | 152, 289 |
| NsiI | ATGCAT | 2 cut(s) | 566, 570 |
| NspI | RCATGY | 2 cut(s) | 564, 568 |
| NspV | TTCGAA | 1 cut(s) | 639 |
| PaeI | GCATGC | 2 cut(s) | 564, 568 |
| PctI | GAATGC | 1 cut(s) | 796 |
| PfeI | GAWTC | 4 cut(s) | 476, 669, 766, 997 |
| PfoI | TCCNGGA | 1 cut(s) | 471 |
| PkrI | GCNGC | 4 cut(s) | 389, 398, 515, 599 |
| PleI | GAGTC | 2 cut(s) | 89, 844 |
| PpsI | GAGTC | 2 cut(s) | 89, 844 |
| PpuMI | RGGWCCY | 1 cut(s) | 469 |
| Psp5II | RGGWCCY | 1 cut(s) | 469 |
| Psp6I | CCWGG | 1 cut(s) | 471 |
| PspGI | CCWGG | 1 cut(s) | 471 |
| PspN4I | GGNNCC | 1 cut(s) | 58 |
| PspPI | GGNCC | 1 cut(s) | 469 |
| PspPPI | RGGWCCY | 1 cut(s) | 469 |
| PstI | CTGCAG | 1 cut(s) | 398 |
| PsuI | RGATCY | 1 cut(s) | 56 |
| PvuII | CAGCTG | 1 cut(s) | 988 |
| RsaI | GTAC | 4 cut(s) | 32, 484, 509, 862 |
| RsaNI | GTAC | 4 cut(s) | 31, 483, 508, 861 |
| RseI | CAYNNNNRTG | 2 cut(s) | 350, 918 |
| SalI | GTCGAC | 1 cut(s) | 15 |
| SaqAI | TTAA | 4 cut(s) | 285, 296, 341, 626 |
| SatI | GCNGC | 4 cut(s) | 388, 397, 514, 598 |
| Sau3AI | GATC | 5 cut(s) | 56, 157, 663, 974, 982 |
| Sau96I | GGNCC | 1 cut(s) | 469 |
| SchI | GAGTC | 2 cut(s) | 90, 845 |
| ScrFI | CCNGG | 1 cut(s) | 473 |
| SduI | GDGCHC | 1 cut(s) | 378 |
| SfcI | CTRYAG | 1 cut(s) | 394 |
| SfuI | TTCGAA | 1 cut(s) | 639 |
| SinI | GGWCC | 1 cut(s) | 469 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 350, 918 |
| SphI | GCATGC | 2 cut(s) | 564, 568 |
| Sse9I | AATT | 4 cut(s) | 256, 324, 486, 602 |
| SsiI | CCGC | 1 cut(s) | 73 |
| SspMI | CTAG | 5 cut(s) | 149, 161, 444, 819, 858 |
| StyD4I | CCNGG | 1 cut(s) | 471 |
| TaaI | ACNGT | 2 cut(s) | 683, 888 |
| TaiI | ACGT | 1 cut(s) | 291 |
| TaqI | TCGA | 6 cut(s) | 16, 88, 496, 639, 777, 963 |
| TasI | AATT | 4 cut(s) | 256, 324, 486, 602 |
| TatI | WGTACW | 1 cut(s) | 482 |
| TfiI | GAWTC | 4 cut(s) | 476, 669, 766, 997 |
| Tru1I | TTAA | 4 cut(s) | 285, 296, 341, 626 |
| Tru9I | TTAA | 4 cut(s) | 285, 296, 341, 626 |
| TscAI | CASTG | 3 cut(s) | 357, 365, 801 |
| TseFI | GTSAC | 2 cut(s) | 152, 289 |
| TseI | GCWGC | 4 cut(s) | 387, 396, 513, 597 |
| Tsp45I | GTSAC | 2 cut(s) | 152, 289 |
| TspGWI | ACGGA | 2 cut(s) | 74, 882 |
| TspRI | CASTG | 3 cut(s) | 357, 365, 801 |
| VpaK11BI | GGWCC | 1 cut(s) | 469 |
| XapI | RAATTY | 1 cut(s) | 602 |
| XceI | RCATGY | 2 cut(s) | 564, 568 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 920 |
| XmiI | GTMKAC | 1 cut(s) | 16 |
| XspI | CTAG | 5 cut(s) | 149, 161, 444, 819, 858 |
| Zsp2I | ATGCAT | 2 cut(s) | 566, 570 |