E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 410 |
| Acc16I | TGCGCA | 1 cut(s) | 788 |
| AccBSI | CCGCTC | 1 cut(s) | 172 |
| AccI | GTMKAC | 1 cut(s) | 87 |
| AccII | CGCG | 1 cut(s) | 582 |
| AciI | CCGC | 1 cut(s) | 170 |
| AclWI | GGATC | 3 cut(s) | 570, 660, 739 |
| AcsI | RAATTY | 2 cut(s) | 238, 791 |
| AdeI | CACNNNGTG | 2 cut(s) | 595, 742 |
| AfaI | GTAC | 1 cut(s) | 73 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 101 |
| AgsI | TTSAA | 5 cut(s) | 254, 280, 343, 435, 719 |
| AluBI | AGCT | 2 cut(s) | 51, 809 |
| AluI | AGCT | 2 cut(s) | 51, 809 |
| Alw21I | GWGCWC | 1 cut(s) | 464 |
| Alw26I | GTCTC | 1 cut(s) | 430 |
| AlwI | GGATC | 3 cut(s) | 570, 660, 739 |
| Ama87I | CYCGRG | 1 cut(s) | 101 |
| ApeKI | GCWGC | 2 cut(s) | 330, 886 |
| ApoI | RAATTY | 2 cut(s) | 238, 791 |
| AspLEI | GCGC | 3 cut(s) | 514, 584, 789 |
| AsuC2I | CCSGG | 3 cut(s) | 102, 103, 603 |
| AsuHPI | GGTGA | 5 cut(s) | 207, 617, 862, 937, 944 |
| AsuII | TTCGAA | 2 cut(s) | 187, 242 |
| AvaI | CYCGRG | 1 cut(s) | 101 |
| AxyI | CCTNAGG | 1 cut(s) | 891 |
| BbsI | GAAGAC | 1 cut(s) | 22 |
| Bbv12I | GWGCWC | 1 cut(s) | 464 |
| BbvI | GCAGC | 2 cut(s) | 317, 898 |
| BccI | CCATC | 3 cut(s) | 667, 922, 940 |
| BceAI | ACGGC | 1 cut(s) | 83 |
| BclI | TGATCA | 1 cut(s) | 934 |
| BcnI | CCSGG | 3 cut(s) | 102, 103, 603 |
| BcoDI | GTCTC | 1 cut(s) | 430 |
| BfaI | CTAG | 4 cut(s) | 96, 314, 806, 810 |
| BfmI | CTRYAG | 2 cut(s) | 372, 799 |
| BglII | AGATCT | 1 cut(s) | 872 |
| BisI | GCNGC | 2 cut(s) | 331, 887 |
| BlsI | GCNGC | 2 cut(s) | 332, 888 |
| Bme1390I | CCNGG | 3 cut(s) | 102, 103, 603 |
| BmeT110I | CYCGRG | 1 cut(s) | 101 |
| BmiI | GGNNCC | 1 cut(s) | 777 |
| BmrFI | CCNGG | 3 cut(s) | 102, 103, 603 |
| BmsI | GCATC | 4 cut(s) | 319, 341, 342, 377 |
| BoxI | GACNNNNGTC | 1 cut(s) | 96 |
| BpiI | GAAGAC | 1 cut(s) | 22 |
| Bpu14I | TTCGAA | 2 cut(s) | 187, 242 |
| BpuEI | CTTGAG | 1 cut(s) | 861 |
| BpuMI | CCSGG | 3 cut(s) | 102, 103, 603 |
| BsaAI | YACGTR | 1 cut(s) | 468 |
| BsaJI | CCNNGG | 3 cut(s) | 101, 102, 396 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 101 |
| Bse21I | CCTNAGG | 1 cut(s) | 891 |
| BseDI | CCNNGG | 3 cut(s) | 101, 102, 396 |
| BseGI | GGATG | 2 cut(s) | 166, 385 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 101 |
| BseMII | CTCAG | 3 cut(s) | 144, 829, 905 |
| BseRI | GAGGAG | 2 cut(s) | 45, 170 |
| BseXI | GCAGC | 2 cut(s) | 317, 898 |
| Bsh1236I | CGCG | 1 cut(s) | 582 |
| Bsh1285I | CGRYCG | 1 cut(s) | 631 |
| BsiEI | CGRYCG | 1 cut(s) | 631 |
| BsiHKAI | GWGCWC | 1 cut(s) | 464 |
| BsiHKCI | CYCGRG | 1 cut(s) | 101 |
| BsiSI | CCGG | 2 cut(s) | 102, 602 |
| BslFI | GGGAC | 3 cut(s) | 84, 243, 510 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 101 |
| BsmAI | GTCTC | 1 cut(s) | 430 |
| BsmFI | GGGAC | 3 cut(s) | 84, 243, 510 |
| BsoBI | CYCGRG | 1 cut(s) | 101 |
| Bsp119I | TTCGAA | 2 cut(s) | 187, 242 |
| Bsp1286I | GDGCHC | 1 cut(s) | 464 |
| Bsp143I | GATC | 5 cut(s) | 562, 652, 731, 872, 934 |
| Bsp19I | CCATGG | 1 cut(s) | 396 |
| BspACI | CCGC | 1 cut(s) | 170 |
| BspCNI | CTCAG | 3 cut(s) | 145, 828, 904 |
| BspFNI | CGCG | 1 cut(s) | 582 |
| BspLI | GGNNCC | 1 cut(s) | 777 |
| BspMAI | CTGCAG | 1 cut(s) | 803 |
| BspPI | GGATC | 3 cut(s) | 570, 660, 739 |
| BspQI | GCTCTTC | 1 cut(s) | 876 |
| BspT104I | TTCGAA | 2 cut(s) | 187, 242 |
| BsrBI | CCGCTC | 1 cut(s) | 172 |
| BssECI | CCNNGG | 3 cut(s) | 101, 102, 396 |
| BssMI | GATC | 5 cut(s) | 562, 652, 731, 872, 934 |
| BssT1I | CCWWGG | 1 cut(s) | 396 |
| Bst4CI | ACNGT | 4 cut(s) | 47, 632, 754, 822 |
| Bst6I | CTCTTC | 5 cut(s) | 53, 108, 341, 684, 876 |
| BstBAI | YACGTR | 1 cut(s) | 468 |
| BstBI | TTCGAA | 2 cut(s) | 187, 242 |
| BstDEI | CTNAG | 4 cut(s) | 153, 815, 833, 891 |
| BstDSI | CCRYGG | 1 cut(s) | 396 |
| BstF5I | GGATG | 2 cut(s) | 166, 385 |
| BstFNI | CGCG | 1 cut(s) | 582 |
| BstHHI | GCGC | 3 cut(s) | 514, 584, 789 |
| BstKTI | GATC | 5 cut(s) | 565, 655, 734, 875, 937 |
| BstMAI | GTCTC | 1 cut(s) | 430 |
| BstMBI | GATC | 5 cut(s) | 562, 652, 731, 872, 934 |
| BstMCI | CGRYCG | 1 cut(s) | 631 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 48, 360 |
| BstNSI | RCATGY | 1 cut(s) | 195 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 96 |
| BstSCI | CCNGG | 3 cut(s) | 100, 101, 601 |
| BstSFI | CTRYAG | 2 cut(s) | 372, 799 |
| BstUI | CGCG | 1 cut(s) | 582 |
| BstV1I | GCAGC | 2 cut(s) | 317, 898 |
| BstV2I | GAAGAC | 1 cut(s) | 22 |
| BstX2I | RGATCY | 3 cut(s) | 562, 652, 872 |
| BstYI | RGATCY | 3 cut(s) | 562, 652, 872 |
| Bsu36I | CCTNAGG | 1 cut(s) | 891 |
| BtgI | CCRYGG | 1 cut(s) | 396 |
| BtsCI | GGATG | 2 cut(s) | 166, 385 |
| BtsI | GCAGTG | 1 cut(s) | 670 |
| BtsIMutI | CAGTG | 2 cut(s) | 302, 670 |
| CfoI | GCGC | 3 cut(s) | 514, 584, 789 |
| Cfr9I | CCCGGG | 1 cut(s) | 101 |
| CseI | GACGC | 1 cut(s) | 27 |
| Csp6I | GTAC | 1 cut(s) | 72 |
| CviAII | CATG | 7 cut(s) | 192, 397, 429, 491, 516, 522, 940 |
| CviJI | RGCY | 9 cut(s) | 42, 51, 395, 489, 552, 648, 759, 809, 889 |
| CviKI_1 | RGCY | 9 cut(s) | 42, 51, 395, 489, 552, 648, 759, 809, 889 |
| CviQI | GTAC | 1 cut(s) | 72 |
| DdeI | CTNAG | 4 cut(s) | 153, 815, 833, 891 |
| DpnI | GATC | 5 cut(s) | 564, 654, 733, 874, 936 |
| DpnII | GATC | 5 cut(s) | 562, 652, 731, 872, 934 |
| DraIII | CACNNNGTG | 2 cut(s) | 595, 742 |
| Eam1104I | CTCTTC | 5 cut(s) | 53, 108, 341, 684, 876 |
| EarI | CTCTTC | 5 cut(s) | 53, 108, 341, 684, 876 |
| Eco130I | CCWWGG | 1 cut(s) | 396 |
| Eco32I | GATATC | 1 cut(s) | 610 |
| Eco81I | CCTNAGG | 1 cut(s) | 891 |
| Eco88I | CYCGRG | 1 cut(s) | 101 |
| EcoRI | GAATTC | 1 cut(s) | 238 |
| EcoRV | GATATC | 1 cut(s) | 610 |
| EcoT14I | CCWWGG | 1 cut(s) | 396 |
| ErhI | CCWWGG | 1 cut(s) | 396 |
| FaeI | CATG | 7 cut(s) | 195, 400, 432, 494, 519, 525, 943 |
| FaqI | GGGAC | 3 cut(s) | 84, 243, 510 |
| FatI | CATG | 7 cut(s) | 191, 396, 428, 490, 515, 521, 939 |
| FbaI | TGATCA | 1 cut(s) | 934 |
| FblI | GTMKAC | 1 cut(s) | 87 |
| Fnu4HI | GCNGC | 2 cut(s) | 331, 887 |
| FokI | GGATG | 2 cut(s) | 173, 392 |
| Fsp4HI | GCNGC | 2 cut(s) | 331, 887 |
| FspBI | CTAG | 4 cut(s) | 96, 314, 806, 810 |
| FspI | TGCGCA | 1 cut(s) | 788 |
| GlaI | GCGC | 3 cut(s) | 513, 583, 788 |
| GluI | GCNGC | 2 cut(s) | 331, 887 |
| HapII | CCGG | 2 cut(s) | 102, 602 |
| HgaI | GACGC | 1 cut(s) | 27 |
| HhaI | GCGC | 3 cut(s) | 514, 584, 789 |
| Hin1II | CATG | 7 cut(s) | 195, 400, 432, 494, 519, 525, 943 |
| Hin6I | GCGC | 3 cut(s) | 512, 582, 787 |
| HinP1I | GCGC | 3 cut(s) | 512, 582, 787 |
| HincII | GTYRAC | 1 cut(s) | 88 |
| HindII | GTYRAC | 1 cut(s) | 88 |
| HinfI | GANTC | 5 cut(s) | 149, 598, 703, 813, 910 |
| HpaII | CCGG | 2 cut(s) | 102, 602 |
| HphI | GGTGA | 5 cut(s) | 207, 617, 862, 937, 944 |
| Hpy166II | GTNNAC | 2 cut(s) | 88, 455 |
| Hpy188I | TCNGA | 5 cut(s) | 154, 176, 731, 818, 989 |
| Hpy188III | TCNNGA | 6 cut(s) | 235, 251, 656, 779, 878, 893 |
| Hpy8I | GTNNAC | 2 cut(s) | 88, 455 |
| Hpy99I | CGWCG | 4 cut(s) | 29, 89, 92, 754 |
| HpyAV | CCTTC | 1 cut(s) | 397 |
| HpyCH4III | ACNGT | 4 cut(s) | 47, 632, 754, 822 |
| HpyCH4IV | ACGT | 1 cut(s) | 467 |
| HpyCH4V | TGCA | 8 cut(s) | 195, 333, 363, 390, 440, 484, 767, 801 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 48, 360 |
| HpyF3I | CTNAG | 4 cut(s) | 153, 815, 833, 891 |
| HpySE526I | ACGT | 1 cut(s) | 467 |
| Hsp92II | CATG | 7 cut(s) | 195, 400, 432, 494, 519, 525, 943 |
| HspAI | GCGC | 3 cut(s) | 512, 582, 787 |
| Ksp22I | TGATCA | 1 cut(s) | 934 |
| Kzo9I | GATC | 5 cut(s) | 562, 652, 731, 872, 934 |
| LguI | GCTCTTC | 1 cut(s) | 876 |
| LpnPI | CCDG | 7 cut(s) | 115, 402, 615, 641, 648, 811, 878 |
| Lsp1109I | GCAGC | 2 cut(s) | 317, 898 |
| LweI | GCATC | 4 cut(s) | 319, 341, 342, 377 |
| MaeI | CTAG | 4 cut(s) | 96, 314, 806, 810 |
| MaeII | ACGT | 1 cut(s) | 467 |
| MaeIII | GTNAC | 2 cut(s) | 226, 539 |
| MalI | GATC | 5 cut(s) | 564, 654, 733, 874, 936 |
| MbiI | CCGCTC | 1 cut(s) | 172 |
| MboI | GATC | 5 cut(s) | 562, 652, 731, 872, 934 |
| MfeI | CAATTG | 1 cut(s) | 262 |
| MflI | RGATCY | 3 cut(s) | 562, 652, 872 |
| MhlI | GDGCHC | 1 cut(s) | 464 |
| MluCI | AATT | 7 cut(s) | 8, 238, 262, 338, 365, 791, 866 |
| MlyI | GAGTC | 2 cut(s) | 607, 807 |
| MnlI | CCTC | 9 cut(s) | 23, 26, 54, 148, 151, 198, 841, 889, 900 |
| MslI | CAYNNNNRTG | 2 cut(s) | 520, 944 |
| MspI | CCGG | 2 cut(s) | 102, 602 |
| MspR9I | CCNGG | 3 cut(s) | 102, 103, 603 |
| MunI | CAATTG | 1 cut(s) | 262 |
| MvnI | CGCG | 1 cut(s) | 582 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 48, 360 |
| NciI | CCSGG | 3 cut(s) | 102, 103, 603 |
| NcoI | CCATGG | 1 cut(s) | 396 |
| NdeII | GATC | 5 cut(s) | 562, 652, 731, 872, 934 |
| NlaIII | CATG | 7 cut(s) | 195, 400, 432, 494, 519, 525, 943 |
| NlaIV | GGNNCC | 1 cut(s) | 777 |
| NsbI | TGCGCA | 1 cut(s) | 788 |
| NspI | RCATGY | 1 cut(s) | 195 |
| NspV | TTCGAA | 2 cut(s) | 187, 242 |
| PciSI | GCTCTTC | 1 cut(s) | 876 |
| PfeI | GAWTC | 3 cut(s) | 149, 703, 910 |
| PkrI | GCNGC | 2 cut(s) | 332, 888 |
| PleI | GAGTC | 2 cut(s) | 606, 807 |
| PpsI | GAGTC | 2 cut(s) | 606, 807 |
| Ppu21I | YACGTR | 1 cut(s) | 468 |
| PshAI | GACNNNNGTC | 1 cut(s) | 96 |
| PsiI | TTATAA | 1 cut(s) | 410 |
| PspN4I | GGNNCC | 1 cut(s) | 777 |
| PstI | CTGCAG | 1 cut(s) | 803 |
| PsuI | RGATCY | 3 cut(s) | 562, 652, 872 |
| RsaI | GTAC | 1 cut(s) | 73 |
| RsaNI | GTAC | 1 cut(s) | 72 |
| RseI | CAYNNNNRTG | 2 cut(s) | 520, 944 |
| SalI | GTCGAC | 1 cut(s) | 86 |
| SapI | GCTCTTC | 1 cut(s) | 876 |
| SatI | GCNGC | 2 cut(s) | 331, 887 |
| Sau3AI | GATC | 5 cut(s) | 562, 652, 731, 872, 934 |
| SchI | GAGTC | 2 cut(s) | 607, 807 |
| ScrFI | CCNGG | 3 cut(s) | 102, 103, 603 |
| SduI | GDGCHC | 1 cut(s) | 464 |
| SetI | ASST | 7 cut(s) | 53, 408, 470, 724, 772, 811, 852 |
| SfaNI | GCATC | 4 cut(s) | 319, 341, 342, 377 |
| SfcI | CTRYAG | 2 cut(s) | 372, 799 |
| SfuI | TTCGAA | 2 cut(s) | 187, 242 |
| SgrDI | CGTCGACG | 1 cut(s) | 86 |
| SmaI | CCCGGG | 1 cut(s) | 103 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 520, 944 |
| SmlI | CTYRAG | 1 cut(s) | 876 |
| SmoI | CTYRAG | 1 cut(s) | 876 |
| Sse9I | AATT | 7 cut(s) | 8, 238, 262, 338, 365, 791, 866 |
| SsiI | CCGC | 1 cut(s) | 170 |
| SspMI | CTAG | 4 cut(s) | 96, 314, 806, 810 |
| StyD4I | CCNGG | 3 cut(s) | 100, 101, 601 |
| StyI | CCWWGG | 1 cut(s) | 396 |
| TaaI | ACNGT | 4 cut(s) | 47, 632, 754, 822 |
| TaiI | ACGT | 1 cut(s) | 470 |
| TaqI | TCGA | 6 cut(s) | 87, 187, 242, 270, 385, 627 |
| TasI | AATT | 7 cut(s) | 8, 238, 262, 338, 365, 791, 866 |
| TfiI | GAWTC | 3 cut(s) | 149, 703, 910 |
| TscAI | CASTG | 2 cut(s) | 302, 670 |
| TseI | GCWGC | 2 cut(s) | 330, 886 |
| TspDTI | ATGAA | 4 cut(s) | 201, 510, 715, 956 |
| TspGWI | ACGGA | 4 cut(s) | 42, 73, 214, 217 |
| TspMI | CCCGGG | 1 cut(s) | 101 |
| TspRI | CASTG | 2 cut(s) | 302, 670 |
| XapI | RAATTY | 2 cut(s) | 238, 791 |
| XceI | RCATGY | 1 cut(s) | 195 |
| XmaI | CCCGGG | 1 cut(s) | 101 |
| XmiI | GTMKAC | 1 cut(s) | 87 |
| XspI | CTAG | 4 cut(s) | 96, 314, 806, 810 |