E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 641 |
| AatII | GACGTC | 1 cut(s) | 13 |
| Acc36I | ACCTGC | 1 cut(s) | 641 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 164 |
| AciI | CCGC | 1 cut(s) | 1205 |
| AclWI | GGATC | 4 cut(s) | 111, 678, 691, 1016 |
| AcoI | YGGCCR | 1 cut(s) | 54 |
| AcsI | RAATTY | 1 cut(s) | 1039 |
| AcyI | GRCGYC | 1 cut(s) | 10 |
| AfaI | GTAC | 2 cut(s) | 476, 752 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 164, 836 |
| AflIII | ACRYGT | 1 cut(s) | 803 |
| AgsI | TTSAA | 4 cut(s) | 82, 519, 791, 1087 |
| AleI | CACNNNNGTG | 1 cut(s) | 767 |
| AluBI | AGCT | 4 cut(s) | 548, 1056, 1071, 1237 |
| AluI | AGCT | 4 cut(s) | 548, 1056, 1071, 1237 |
| Alw26I | GTCTC | 3 cut(s) | 812, 856, 1140 |
| AlwI | GGATC | 4 cut(s) | 111, 678, 691, 1016 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 25 |
| Ama87I | CYCGRG | 2 cut(s) | 765, 1238 |
| AoxI | GGCC | 1 cut(s) | 54 |
| ApeKI | GCWGC | 4 cut(s) | 494, 636, 756, 840 |
| ApoI | RAATTY | 1 cut(s) | 1039 |
| AspS9I | GGNCC | 1 cut(s) | 470 |
| AsuC2I | CCSGG | 1 cut(s) | 215 |
| AsuHPI | GGTGA | 1 cut(s) | 1057 |
| AvaI | CYCGRG | 2 cut(s) | 765, 1238 |
| AvaII | GGWCC | 1 cut(s) | 470 |
| BaeGI | GKGCMC | 1 cut(s) | 662 |
| BamHI | GGATCC | 1 cut(s) | 683 |
| BanII | GRGCYC | 1 cut(s) | 579 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 82, 114 |
| BauI | CACGAG | 1 cut(s) | 1109 |
| BbsI | GAAGAC | 5 cut(s) | 216, 243, 309, 375, 586 |
| BbvI | GCAGC | 4 cut(s) | 481, 623, 743, 827 |
| BccI | CCATC | 7 cut(s) | 128, 158, 452, 493, 593, 989, 1237 |
| BceAI | ACGGC | 2 cut(s) | 686, 769 |
| BciVI | GTATCC | 1 cut(s) | 455 |
| BclI | TGATCA | 1 cut(s) | 604 |
| BcnI | CCSGG | 1 cut(s) | 215 |
| BcoDI | GTCTC | 3 cut(s) | 812, 856, 1140 |
| BfaI | CTAG | 4 cut(s) | 230, 296, 362, 431 |
| BfuAI | ACCTGC | 1 cut(s) | 641 |
| BfuI | GTATCC | 1 cut(s) | 455 |
| BisI | GCNGC | 4 cut(s) | 495, 637, 757, 841 |
| BlsI | GCNGC | 4 cut(s) | 496, 638, 758, 842 |
| Bme1390I | CCNGG | 1 cut(s) | 215 |
| Bme18I | GGWCC | 1 cut(s) | 470 |
| BmeT110I | CYCGRG | 2 cut(s) | 765, 1238 |
| BmgT120I | GGNCC | 1 cut(s) | 470 |
| BmiI | GGNNCC | 1 cut(s) | 685 |
| BmrFI | CCNGG | 1 cut(s) | 215 |
| BpiI | GAAGAC | 5 cut(s) | 216, 243, 309, 375, 586 |
| BpmI | CTGGAG | 1 cut(s) | 97 |
| BpuMI | CCSGG | 1 cut(s) | 215 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1247 |
| BsaHI | GRCGYC | 1 cut(s) | 10 |
| BsaI | GGTCTC | 1 cut(s) | 1140 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 902, 932 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 164, 836 |
| Bse1I | ACTGG | 2 cut(s) | 598, 1188 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1247 |
| BseGI | GGATG | 5 cut(s) | 169, 485, 585, 1184, 1234 |
| BseJI | GATNNNNATC | 1 cut(s) | 1247 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 164, 836 |
| BseMII | CTCAG | 2 cut(s) | 17, 531 |
| BseNI | ACTGG | 2 cut(s) | 598, 1188 |
| BseSI | GKGCMC | 1 cut(s) | 662 |
| BseX3I | CGGCCG | 1 cut(s) | 54 |
| BseXI | GCAGC | 4 cut(s) | 481, 623, 743, 827 |
| BseYI | CCCAGC | 1 cut(s) | 1067 |
| BsgI | GTGCAG | 2 cut(s) | 622, 826 |
| Bsh1285I | CGRYCG | 1 cut(s) | 57 |
| BshFI | GGCC | 1 cut(s) | 56 |
| BsiEI | CGRYCG | 1 cut(s) | 57 |
| BsiHKCI | CYCGRG | 2 cut(s) | 765, 1238 |
| BsiSI | CCGG | 1 cut(s) | 215 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 164, 836 |
| BsmAI | GTCTC | 3 cut(s) | 812, 856, 1140 |
| BsnI | GGCC | 1 cut(s) | 56 |
| Bso31I | GGTCTC | 1 cut(s) | 1140 |
| BsoBI | CYCGRG | 2 cut(s) | 765, 1238 |
| Bsp1286I | GDGCHC | 2 cut(s) | 579, 662 |
| Bsp143I | GATC | 8 cut(s) | 29, 116, 172, 277, 343, 604, 683, 1021 |
| BspACI | CCGC | 1 cut(s) | 1205 |
| BspANI | GGCC | 1 cut(s) | 56 |
| BspCNI | CTCAG | 2 cut(s) | 18, 532 |
| BspLI | GGNNCC | 1 cut(s) | 685 |
| BspMI | ACCTGC | 1 cut(s) | 641 |
| BspPI | GGATC | 4 cut(s) | 111, 678, 691, 1016 |
| BspQI | GCTCTTC | 3 cut(s) | 246, 312, 378 |
| BspTNI | GGTCTC | 1 cut(s) | 1140 |
| BsrI | ACTGG | 2 cut(s) | 598, 1188 |
| BssMI | GATC | 8 cut(s) | 29, 116, 172, 277, 343, 604, 683, 1021 |
| BssNI | GRCGYC | 1 cut(s) | 10 |
| BssSI | CACGAG | 1 cut(s) | 1109 |
| Bst2BI | CACGAG | 1 cut(s) | 1109 |
| Bst4CI | ACNGT | 1 cut(s) | 474 |
| Bst6I | CTCTTC | 7 cut(s) | 189, 246, 255, 312, 321, 378, 387 |
| BstACI | GRCGYC | 1 cut(s) | 10 |
| BstC8I | GCNNGC | 1 cut(s) | 546 |
| BstDEI | CTNAG | 3 cut(s) | 26, 540, 1052 |
| BstF5I | GGATG | 5 cut(s) | 169, 485, 585, 1184, 1234 |
| BstKTI | GATC | 8 cut(s) | 32, 119, 175, 280, 346, 607, 686, 1024 |
| BstMAI | GTCTC | 3 cut(s) | 812, 856, 1140 |
| BstMBI | GATC | 8 cut(s) | 29, 116, 172, 277, 343, 604, 683, 1021 |
| BstMCI | CGRYCG | 1 cut(s) | 57 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 550, 567, 633 |
| BstNSI | RCATGY | 1 cut(s) | 807 |
| BstSCI | CCNGG | 1 cut(s) | 213 |
| BstSLI | GKGCMC | 1 cut(s) | 662 |
| BstV1I | GCAGC | 4 cut(s) | 481, 623, 743, 827 |
| BstV2I | GAAGAC | 5 cut(s) | 216, 243, 309, 375, 586 |
| BstX2I | RGATCY | 2 cut(s) | 116, 683 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1075 |
| BstYI | RGATCY | 2 cut(s) | 116, 683 |
| BstZI | CGGCCG | 1 cut(s) | 54 |
| BsuI | GTATCC | 1 cut(s) | 455 |
| BsuRI | GGCC | 1 cut(s) | 56 |
| BtsCI | GGATG | 5 cut(s) | 169, 485, 585, 1184, 1234 |
| BtsIMutI | CAGTG | 1 cut(s) | 605 |
| BveI | ACCTGC | 1 cut(s) | 641 |
| Cac8I | GCNNGC | 1 cut(s) | 546 |
| CaiI | CAGNNNCTG | 1 cut(s) | 25 |
| Cfr13I | GGNCC | 1 cut(s) | 470 |
| Csp6I | GTAC | 2 cut(s) | 475, 751 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 507, 542 |
| CviAII | CATG | 9 cut(s) | 47, 512, 735, 794, 804, 825, 893, 1075, 1208 |
| CviQI | GTAC | 2 cut(s) | 475, 751 |
| DdeI | CTNAG | 3 cut(s) | 26, 540, 1052 |
| DpnI | GATC | 8 cut(s) | 31, 118, 174, 279, 345, 606, 685, 1023 |
| DpnII | GATC | 8 cut(s) | 29, 116, 172, 277, 343, 604, 683, 1021 |
| EaeI | YGGCCR | 1 cut(s) | 54 |
| EagI | CGGCCG | 1 cut(s) | 54 |
| Eam1104I | CTCTTC | 7 cut(s) | 189, 246, 255, 312, 321, 378, 387 |
| EarI | CTCTTC | 7 cut(s) | 189, 246, 255, 312, 321, 378, 387 |
| EclXI | CGGCCG | 1 cut(s) | 54 |
| Eco24I | GRGCYC | 1 cut(s) | 579 |
| Eco31I | GGTCTC | 1 cut(s) | 1140 |
| Eco47I | GGWCC | 1 cut(s) | 470 |
| Eco52I | CGGCCG | 1 cut(s) | 54 |
| Eco88I | CYCGRG | 2 cut(s) | 765, 1238 |
| EcoT38I | GRGCYC | 1 cut(s) | 579 |
| FaeI | CATG | 9 cut(s) | 50, 515, 738, 797, 807, 828, 896, 1078, 1211 |
| FatI | CATG | 9 cut(s) | 46, 511, 734, 793, 803, 824, 892, 1074, 1207 |
| FauI | CCCGC | 1 cut(s) | 1212 |
| FbaI | TGATCA | 1 cut(s) | 604 |
| Fnu4HI | GCNGC | 4 cut(s) | 495, 637, 757, 841 |
| FokI | GGATG | 5 cut(s) | 176, 472, 572, 1171, 1241 |
| FriOI | GRGCYC | 1 cut(s) | 579 |
| Fsp4HI | GCNGC | 4 cut(s) | 495, 637, 757, 841 |
| FspBI | CTAG | 4 cut(s) | 230, 296, 362, 431 |
| GluI | GCNGC | 4 cut(s) | 495, 637, 757, 841 |
| GsaI | CCCAGC | 1 cut(s) | 1071 |
| GsuI | CTGGAG | 1 cut(s) | 97 |
| HaeIII | GGCC | 1 cut(s) | 56 |
| HapII | CCGG | 1 cut(s) | 215 |
| Hin1I | GRCGYC | 1 cut(s) | 10 |
| Hin1II | CATG | 9 cut(s) | 50, 515, 738, 797, 807, 828, 896, 1078, 1211 |
| HinfI | GANTC | 5 cut(s) | 16, 50, 719, 1048, 1220 |
| HpaII | CCGG | 1 cut(s) | 215 |
| HphI | GGTGA | 1 cut(s) | 1057 |
| Hpy188I | TCNGA | 3 cut(s) | 15, 27, 1125 |
| Hpy188III | TCNNGA | 8 cut(s) | 82, 114, 281, 347, 413, 706, 983, 1240 |
| Hpy99I | CGWCG | 4 cut(s) | 62, 707, 926, 929 |
| HpyAV | CCTTC | 4 cut(s) | 194, 443, 461, 672 |
| HpyCH4III | ACNGT | 1 cut(s) | 474 |
| HpyCH4IV | ACGT | 2 cut(s) | 10, 845 |
| HpyCH4V | TGCA | 7 cut(s) | 128, 151, 639, 728, 759, 843, 905 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 550, 567, 633 |
| HpyF3I | CTNAG | 3 cut(s) | 26, 540, 1052 |
| HpySE526I | ACGT | 2 cut(s) | 10, 845 |
| Hsp92I | GRCGYC | 1 cut(s) | 10 |
| Hsp92II | CATG | 9 cut(s) | 50, 515, 738, 797, 807, 828, 896, 1078, 1211 |
| Ksp22I | TGATCA | 1 cut(s) | 604 |
| Kzo9I | GATC | 8 cut(s) | 29, 116, 172, 277, 343, 604, 683, 1021 |
| LguI | GCTCTTC | 3 cut(s) | 246, 312, 378 |
| LmnI | GCTCC | 2 cut(s) | 167, 632 |
| Lsp1109I | GCAGC | 4 cut(s) | 481, 623, 743, 827 |
| MaeI | CTAG | 4 cut(s) | 230, 296, 362, 431 |
| MaeII | ACGT | 2 cut(s) | 10, 845 |
| MaeIII | GTNAC | 3 cut(s) | 182, 614, 1045 |
| MalI | GATC | 8 cut(s) | 31, 118, 174, 279, 345, 606, 685, 1023 |
| MboI | GATC | 8 cut(s) | 29, 116, 172, 277, 343, 604, 683, 1021 |
| MfeI | CAATTG | 1 cut(s) | 723 |
| MflI | RGATCY | 2 cut(s) | 116, 683 |
| MhlI | GDGCHC | 2 cut(s) | 579, 662 |
| MluCI | AATT | 7 cut(s) | 711, 723, 786, 1039, 1127, 1165, 1180 |
| MlyI | GAGTC | 2 cut(s) | 44, 1042 |
| MmeI | TCCRAC | 1 cut(s) | 942 |
| MseI | TTAA | 4 cut(s) | 953, 1104, 1130, 1199 |
| MslI | CAYNNNNRTG | 2 cut(s) | 644, 767 |
| MspI | CCGG | 1 cut(s) | 215 |
| MspR9I | CCNGG | 1 cut(s) | 215 |
| MunI | CAATTG | 1 cut(s) | 723 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 550, 567, 633 |
| NciI | CCSGG | 1 cut(s) | 215 |
| NdeII | GATC | 8 cut(s) | 29, 116, 172, 277, 343, 604, 683, 1021 |
| NlaIII | CATG | 9 cut(s) | 50, 515, 738, 797, 807, 828, 896, 1078, 1211 |
| NlaIV | GGNNCC | 1 cut(s) | 685 |
| NmeAIII | GCCGAG | 2 cut(s) | 32, 722 |
| NmuCI | GTSAC | 3 cut(s) | 182, 614, 1045 |
| NspI | RCATGY | 1 cut(s) | 807 |
| OliI | CACNNNNGTG | 1 cut(s) | 767 |
| PaeR7I | CTCGAG | 2 cut(s) | 765, 1238 |
| PaqCI | CACCTGC | 1 cut(s) | 641 |
| PciI | ACATGT | 1 cut(s) | 803 |
| PciSI | GCTCTTC | 3 cut(s) | 246, 312, 378 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 921, 1026 |
| PfeI | GAWTC | 3 cut(s) | 16, 719, 1220 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 164 |
| PkrI | GCNGC | 4 cut(s) | 496, 638, 758, 842 |
| PleI | GAGTC | 2 cut(s) | 44, 1042 |
| PpsI | GAGTC | 2 cut(s) | 44, 1042 |
| PscI | ACATGT | 1 cut(s) | 803 |
| PspFI | CCCAGC | 1 cut(s) | 1067 |
| PspN4I | GGNNCC | 1 cut(s) | 685 |
| PspPI | GGNCC | 1 cut(s) | 470 |
| PspXI | VCTCGAGB | 1 cut(s) | 765 |
| PstNI | CAGNNNCTG | 1 cut(s) | 25 |
| PsuI | RGATCY | 2 cut(s) | 116, 683 |
| RsaI | GTAC | 2 cut(s) | 476, 752 |
| RsaNI | GTAC | 2 cut(s) | 475, 751 |
| RseI | CAYNNNNRTG | 2 cut(s) | 644, 767 |
| SapI | GCTCTTC | 3 cut(s) | 246, 312, 378 |
| SaqAI | TTAA | 4 cut(s) | 953, 1104, 1130, 1199 |
| SatI | GCNGC | 4 cut(s) | 495, 637, 757, 841 |
| Sau3AI | GATC | 8 cut(s) | 29, 116, 172, 277, 343, 604, 683, 1021 |
| Sau96I | GGNCC | 1 cut(s) | 470 |
| SchI | GAGTC | 2 cut(s) | 44, 1042 |
| ScrFI | CCNGG | 1 cut(s) | 215 |
| SduI | GDGCHC | 2 cut(s) | 579, 662 |
| Sfr274I | CTCGAG | 2 cut(s) | 765, 1238 |
| SinI | GGWCC | 1 cut(s) | 470 |
| SlaI | CTCGAG | 2 cut(s) | 765, 1238 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 644, 767 |
| SmlI | CTYRAG | 2 cut(s) | 765, 1238 |
| SmoI | CTYRAG | 2 cut(s) | 765, 1238 |
| Sse9I | AATT | 7 cut(s) | 711, 723, 786, 1039, 1127, 1165, 1180 |
| SsiI | CCGC | 1 cut(s) | 1205 |
| SspI | AATATT | 1 cut(s) | 523 |
| SspMI | CTAG | 4 cut(s) | 230, 296, 362, 431 |
| StyD4I | CCNGG | 1 cut(s) | 213 |
| TaaI | ACNGT | 1 cut(s) | 474 |
| TaiI | ACGT | 2 cut(s) | 13, 848 |
| TaqI | TCGA | 7 cut(s) | 175, 555, 705, 766, 982, 1218, 1239 |
| TaqII | GACCGA | 1 cut(s) | 516 |
| TasI | AATT | 7 cut(s) | 711, 723, 786, 1039, 1127, 1165, 1180 |
| TfiI | GAWTC | 3 cut(s) | 16, 719, 1220 |
| Tru1I | TTAA | 4 cut(s) | 953, 1104, 1130, 1199 |
| Tru9I | TTAA | 4 cut(s) | 953, 1104, 1130, 1199 |
| TscAI | CASTG | 1 cut(s) | 605 |
| TseFI | GTSAC | 3 cut(s) | 182, 614, 1045 |
| TseI | GCWGC | 4 cut(s) | 494, 636, 756, 840 |
| Tsp45I | GTSAC | 3 cut(s) | 182, 614, 1045 |
| TspGWI | ACGGA | 5 cut(s) | 146, 282, 348, 414, 923 |
| TspRI | CASTG | 1 cut(s) | 605 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 164 |
| VpaK11BI | GGWCC | 1 cut(s) | 470 |
| XapI | RAATTY | 1 cut(s) | 1039 |
| XceI | RCATGY | 1 cut(s) | 807 |
| XhoI | CTCGAG | 2 cut(s) | 765, 1238 |
| XspI | CTAG | 4 cut(s) | 230, 296, 362, 431 |
| ZraI | GACGTC | 1 cut(s) | 11 |