E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1160 |
| AccB1I | GGYRCC | 1 cut(s) | 358 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 415 |
| AccII | CGCG | 2 cut(s) | 14, 364 |
| AciI | CCGC | 6 cut(s) | 108, 152, 329, 362, 364, 391 |
| AclWI | GGATC | 4 cut(s) | 42, 55, 374, 1209 |
| AcoI | YGGCCR | 1 cut(s) | 429 |
| AcsI | RAATTY | 4 cut(s) | 340, 705, 935, 1006 |
| AdeI | CACNNNGTG | 1 cut(s) | 415 |
| AfaI | GTAC | 1 cut(s) | 354 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 335, 355, 415, 648, 811, 812 |
| AgsI | TTSAA | 5 cut(s) | 834, 1031, 1042, 1146, 1244 |
| AjnI | CCWGG | 3 cut(s) | 59, 647, 1125 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 300, 332 |
| AluBI | AGCT | 4 cut(s) | 113, 175, 599, 1192 |
| AluI | AGCT | 4 cut(s) | 113, 175, 599, 1192 |
| Alw26I | GTCTC | 1 cut(s) | 83 |
| AlwI | GGATC | 4 cut(s) | 42, 55, 374, 1209 |
| AoxI | GGCC | 5 cut(s) | 62, 429, 650, 675, 1123 |
| ApeKI | GCWGC | 5 cut(s) | 110, 321, 612, 732, 816 |
| ApoI | RAATTY | 4 cut(s) | 340, 705, 935, 1006 |
| AspS9I | GGNCC | 3 cut(s) | 63, 651, 675 |
| AsuC2I | CCSGG | 1 cut(s) | 674 |
| AsuHPI | GGTGA | 7 cut(s) | 304, 474, 481, 502, 783, 850, 907 |
| BamHI | GGATCC | 1 cut(s) | 47 |
| BanI | GGYRCC | 1 cut(s) | 358 |
| BbsI | GAAGAC | 3 cut(s) | 294, 499, 565 |
| BbvI | GCAGC | 5 cut(s) | 122, 308, 599, 719, 803 |
| BccI | CCATC | 5 cut(s) | 26, 488, 572, 920, 1043 |
| BciT130I | CCWGG | 3 cut(s) | 61, 649, 1127 |
| BcnI | CCSGG | 1 cut(s) | 674 |
| BcoDI | GTCTC | 1 cut(s) | 83 |
| BfaI | CTAG | 1 cut(s) | 1167 |
| BfmI | CTRYAG | 2 cut(s) | 383, 1119 |
| BisI | GCNGC | 6 cut(s) | 111, 322, 362, 613, 733, 817 |
| BlsI | GCNGC | 6 cut(s) | 112, 323, 363, 614, 734, 818 |
| Bme1390I | CCNGG | 4 cut(s) | 61, 649, 674, 1127 |
| BmgT120I | GGNCC | 3 cut(s) | 63, 651, 675 |
| BmiI | GGNNCC | 2 cut(s) | 49, 360 |
| BmrFI | CCNGG | 4 cut(s) | 61, 649, 674, 1127 |
| BmsI | GCATC | 3 cut(s) | 126, 909, 1244 |
| BpiI | GAAGAC | 3 cut(s) | 294, 499, 565 |
| Bpu10I | CCTNAGC | 1 cut(s) | 514 |
| BpuMI | CCSGG | 1 cut(s) | 674 |
| Bsa29I | ATCGAT | 2 cut(s) | 372, 903 |
| BsaJI | CCNNGG | 2 cut(s) | 59, 362 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 213, 243 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 335, 355, 415, 648, 811, 812 |
| Bse1I | ACTGG | 2 cut(s) | 577, 1099 |
| BseBI | CCWGG | 3 cut(s) | 61, 649, 1127 |
| BseCI | ATCGAT | 2 cut(s) | 372, 903 |
| BseDI | CCNNGG | 2 cut(s) | 59, 362 |
| BseGI | GGATG | 3 cut(s) | 440, 564, 1235 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 335, 355, 415, 648, 811, 812 |
| BseMII | CTCAG | 1 cut(s) | 1105 |
| BseNI | ACTGG | 2 cut(s) | 577, 1099 |
| BseRI | GAGGAG | 2 cut(s) | 462, 595 |
| BseXI | GCAGC | 5 cut(s) | 122, 308, 599, 719, 803 |
| BseYI | CCCAGC | 1 cut(s) | 1231 |
| BsgI | GTGCAG | 1 cut(s) | 598 |
| Bsh1236I | CGCG | 2 cut(s) | 14, 364 |
| BshFI | GGCC | 5 cut(s) | 64, 431, 652, 677, 1125 |
| BshNI | GGYRCC | 1 cut(s) | 358 |
| BshVI | ATCGAT | 2 cut(s) | 372, 903 |
| BsiSI | CCGG | 2 cut(s) | 450, 673 |
| BsiWI | CGTACG | 1 cut(s) | 352 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 335, 355, 415, 648, 811, 812 |
| BsmAI | GTCTC | 1 cut(s) | 83 |
| BsmBI | CGTCTC | 1 cut(s) | 83 |
| BsnI | GGCC | 5 cut(s) | 64, 431, 652, 677, 1125 |
| Bsp143I | GATC | 5 cut(s) | 47, 366, 477, 1060, 1201 |
| BspACI | CCGC | 6 cut(s) | 108, 152, 329, 362, 364, 391 |
| BspANI | GGCC | 5 cut(s) | 64, 431, 652, 677, 1125 |
| BspCNI | CTCAG | 1 cut(s) | 1104 |
| BspDI | ATCGAT | 2 cut(s) | 372, 903 |
| BspFNI | CGCG | 2 cut(s) | 14, 364 |
| BspLI | GGNNCC | 2 cut(s) | 49, 360 |
| BspMAI | CTGCAG | 2 cut(s) | 387, 1123 |
| BspPI | GGATC | 4 cut(s) | 42, 55, 374, 1209 |
| BspT107I | GGYRCC | 1 cut(s) | 358 |
| BsrI | ACTGG | 2 cut(s) | 577, 1099 |
| BssECI | CCNNGG | 2 cut(s) | 59, 362 |
| BssMI | GATC | 5 cut(s) | 47, 366, 477, 1060, 1201 |
| Bst2UI | CCWGG | 3 cut(s) | 61, 649, 1127 |
| Bst4CI | ACNGT | 3 cut(s) | 184, 316, 862 |
| Bst6I | CTCTTC | 2 cut(s) | 255, 835 |
| BstC8I | GCNNGC | 6 cut(s) | 115, 433, 601, 839, 1123, 1190 |
| BstDEI | CTNAG | 2 cut(s) | 514, 1091 |
| BstDSI | CCRYGG | 1 cut(s) | 362 |
| BstF5I | GGATG | 3 cut(s) | 440, 564, 1235 |
| BstFNI | CGCG | 2 cut(s) | 14, 364 |
| BstKTI | GATC | 5 cut(s) | 50, 369, 480, 1063, 1204 |
| BstMAI | GTCTC | 1 cut(s) | 83 |
| BstMBI | GATC | 5 cut(s) | 47, 366, 477, 1060, 1201 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 11, 70, 119, 172, 546, 609 |
| BstNI | CCWGG | 3 cut(s) | 61, 649, 1127 |
| BstNSI | RCATGY | 1 cut(s) | 783 |
| BstSCI | CCNGG | 4 cut(s) | 59, 647, 672, 1125 |
| BstSFI | CTRYAG | 2 cut(s) | 383, 1119 |
| BstUI | CGCG | 2 cut(s) | 14, 364 |
| BstV1I | GCAGC | 5 cut(s) | 122, 308, 599, 719, 803 |
| BstV2I | GAAGAC | 3 cut(s) | 294, 499, 565 |
| BstX2I | RGATCY | 2 cut(s) | 47, 1201 |
| BstYI | RGATCY | 2 cut(s) | 47, 1201 |
| Bsu15I | ATCGAT | 2 cut(s) | 372, 903 |
| BsuRI | GGCC | 5 cut(s) | 64, 431, 652, 677, 1125 |
| BsuTUI | ATCGAT | 2 cut(s) | 372, 903 |
| BtgI | CCRYGG | 1 cut(s) | 362 |
| BtgZI | GCGATG | 1 cut(s) | 103 |
| BtsCI | GGATG | 3 cut(s) | 440, 564, 1235 |
| BtsIMutI | CAGTG | 1 cut(s) | 584 |
| Cac8I | GCNNGC | 6 cut(s) | 115, 433, 601, 839, 1123, 1190 |
| Cfr13I | GGNCC | 3 cut(s) | 63, 651, 675 |
| Cfr42I | CCGCGG | 1 cut(s) | 365 |
| ClaI | ATCGAT | 2 cut(s) | 372, 903 |
| Csp6I | GTAC | 1 cut(s) | 353 |
| CviAII | CATG | 8 cut(s) | 711, 728, 770, 780, 801, 869, 989, 1198 |
| CviQI | GTAC | 1 cut(s) | 353 |
| DdeI | CTNAG | 2 cut(s) | 514, 1091 |
| DpnI | GATC | 5 cut(s) | 49, 368, 479, 1062, 1203 |
| DpnII | GATC | 5 cut(s) | 47, 366, 477, 1060, 1201 |
| DraIII | CACNNNGTG | 1 cut(s) | 415 |
| EaeI | YGGCCR | 1 cut(s) | 429 |
| Eam1104I | CTCTTC | 2 cut(s) | 255, 835 |
| EarI | CTCTTC | 2 cut(s) | 255, 835 |
| EcoRII | CCWGG | 3 cut(s) | 59, 647, 1125 |
| Esp3I | CGTCTC | 1 cut(s) | 83 |
| FaeI | CATG | 8 cut(s) | 714, 731, 773, 783, 804, 872, 992, 1201 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1034, 1066 |
| FatI | CATG | 8 cut(s) | 710, 727, 769, 779, 800, 868, 988, 1197 |
| FauI | CCCGC | 1 cut(s) | 115 |
| FauNDI | CATATG | 1 cut(s) | 1211 |
| Fnu4HI | GCNGC | 6 cut(s) | 111, 322, 362, 613, 733, 817 |
| FokI | GGATG | 3 cut(s) | 427, 551, 1222 |
| Fsp4HI | GCNGC | 6 cut(s) | 111, 322, 362, 613, 733, 817 |
| FspBI | CTAG | 1 cut(s) | 1167 |
| GluI | GCNGC | 6 cut(s) | 111, 322, 362, 613, 733, 817 |
| GsaI | CCCAGC | 1 cut(s) | 1235 |
| HaeIII | GGCC | 5 cut(s) | 64, 431, 652, 677, 1125 |
| HapII | CCGG | 2 cut(s) | 450, 673 |
| Hin1II | CATG | 8 cut(s) | 714, 731, 773, 783, 804, 872, 992, 1201 |
| HinfI | GANTC | 6 cut(s) | 9, 212, 453, 688, 695, 1095 |
| HpaII | CCGG | 2 cut(s) | 450, 673 |
| HphI | GGTGA | 7 cut(s) | 304, 474, 481, 502, 783, 850, 907 |
| Hpy188I | TCNGA | 3 cut(s) | 217, 477, 1094 |
| Hpy188III | TCNNGA | 1 cut(s) | 692 |
| HpyAV | CCTTC | 8 cut(s) | 32, 38, 104, 212, 290, 956, 1114, 1149 |
| HpyCH4III | ACNGT | 3 cut(s) | 184, 316, 862 |
| HpyCH4V | TGCA | 7 cut(s) | 385, 615, 735, 819, 881, 1121, 1209 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 11, 70, 119, 172, 546, 609 |
| HpyF3I | CTNAG | 2 cut(s) | 514, 1091 |
| Hsp92II | CATG | 8 cut(s) | 714, 731, 773, 783, 804, 872, 992, 1201 |
| KspI | CCGCGG | 1 cut(s) | 365 |
| Kzo9I | GATC | 5 cut(s) | 47, 366, 477, 1060, 1201 |
| LmnI | GCTCC | 1 cut(s) | 608 |
| Lsp1109I | GCAGC | 5 cut(s) | 122, 308, 599, 719, 803 |
| LweI | GCATC | 3 cut(s) | 126, 909, 1244 |
| MaeI | CTAG | 1 cut(s) | 1167 |
| MaeIII | GTNAC | 3 cut(s) | 487, 745, 1151 |
| MalI | GATC | 5 cut(s) | 49, 368, 479, 1062, 1203 |
| MboI | GATC | 5 cut(s) | 47, 366, 477, 1060, 1201 |
| MflI | RGATCY | 2 cut(s) | 47, 1201 |
| MluCI | AATT | 5 cut(s) | 340, 699, 705, 935, 1006 |
| MlyI | GAGTC | 1 cut(s) | 462 |
| MmeI | TCCRAC | 1 cut(s) | 468 |
| MseI | TTAA | 3 cut(s) | 920, 927, 1261 |
| MspA1I | CMGCKG | 1 cut(s) | 364 |
| MspI | CCGG | 2 cut(s) | 450, 673 |
| MspR9I | CCNGG | 4 cut(s) | 61, 649, 674, 1127 |
| MvaI | CCWGG | 3 cut(s) | 61, 649, 1127 |
| MvnI | CGCG | 2 cut(s) | 14, 364 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 11, 70, 119, 172, 546, 609 |
| NciI | CCSGG | 1 cut(s) | 674 |
| NdeI | CATATG | 1 cut(s) | 1211 |
| NdeII | GATC | 5 cut(s) | 47, 366, 477, 1060, 1201 |
| NlaIII | CATG | 8 cut(s) | 714, 731, 773, 783, 804, 872, 992, 1201 |
| NlaIV | GGNNCC | 2 cut(s) | 49, 360 |
| NmuCI | GTSAC | 1 cut(s) | 487 |
| NspI | RCATGY | 1 cut(s) | 783 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 126 |
| PfeI | GAWTC | 5 cut(s) | 9, 212, 688, 695, 1095 |
| Pfl23II | CGTACG | 1 cut(s) | 352 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 415 |
| PkrI | GCNGC | 6 cut(s) | 112, 323, 363, 614, 734, 818 |
| PleI | GAGTC | 1 cut(s) | 461 |
| PpsI | GAGTC | 1 cut(s) | 461 |
| PsiI | TTATAA | 1 cut(s) | 1160 |
| Psp6I | CCWGG | 3 cut(s) | 59, 647, 1125 |
| PspFI | CCCAGC | 1 cut(s) | 1231 |
| PspGI | CCWGG | 3 cut(s) | 59, 647, 1125 |
| PspLI | CGTACG | 1 cut(s) | 352 |
| PspN4I | GGNNCC | 2 cut(s) | 49, 360 |
| PspPI | GGNCC | 3 cut(s) | 63, 651, 675 |
| PstI | CTGCAG | 2 cut(s) | 387, 1123 |
| PsuI | RGATCY | 2 cut(s) | 47, 1201 |
| RsaI | GTAC | 1 cut(s) | 354 |
| RsaNI | GTAC | 1 cut(s) | 353 |
| SacII | CCGCGG | 1 cut(s) | 365 |
| SaqAI | TTAA | 3 cut(s) | 920, 927, 1261 |
| SatI | GCNGC | 6 cut(s) | 111, 322, 362, 613, 733, 817 |
| Sau3AI | GATC | 5 cut(s) | 47, 366, 477, 1060, 1201 |
| Sau96I | GGNCC | 3 cut(s) | 63, 651, 675 |
| SchI | GAGTC | 1 cut(s) | 462 |
| ScrFI | CCNGG | 4 cut(s) | 61, 649, 674, 1127 |
| SfaNI | GCATC | 3 cut(s) | 126, 909, 1244 |
| SfcI | CTRYAG | 2 cut(s) | 383, 1119 |
| Sfr303I | CCGCGG | 1 cut(s) | 365 |
| SgrBI | CCGCGG | 1 cut(s) | 365 |
| Sse9I | AATT | 5 cut(s) | 340, 699, 705, 935, 1006 |
| SsiI | CCGC | 6 cut(s) | 108, 152, 329, 362, 364, 391 |
| SspI | AATATT | 1 cut(s) | 925 |
| SspMI | CTAG | 1 cut(s) | 1167 |
| StyD4I | CCNGG | 4 cut(s) | 59, 647, 672, 1125 |
| TaaI | ACNGT | 3 cut(s) | 184, 316, 862 |
| TaqI | TCGA | 3 cut(s) | 129, 372, 903 |
| TasI | AATT | 5 cut(s) | 340, 699, 705, 935, 1006 |
| TauI | GCSGC | 1 cut(s) | 364 |
| TfiI | GAWTC | 5 cut(s) | 9, 212, 688, 695, 1095 |
| Tru1I | TTAA | 3 cut(s) | 920, 927, 1261 |
| Tru9I | TTAA | 3 cut(s) | 920, 927, 1261 |
| TscAI | CASTG | 1 cut(s) | 584 |
| TseFI | GTSAC | 1 cut(s) | 487 |
| TseI | GCWGC | 5 cut(s) | 110, 321, 612, 732, 816 |
| Tsp45I | GTSAC | 1 cut(s) | 487 |
| TspDTI | ATGAA | 8 cut(s) | 42, 210, 222, 567, 699, 780, 786, 789 |
| TspGWI | ACGGA | 2 cut(s) | 193, 1095 |
| TspRI | CASTG | 1 cut(s) | 584 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 415 |
| XapI | RAATTY | 4 cut(s) | 340, 705, 935, 1006 |
| XceI | RCATGY | 1 cut(s) | 783 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 412, 584 |
| XspI | CTAG | 1 cut(s) | 1167 |