E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 838 |
| AciI | CCGC | 1 cut(s) | 65 |
| AcoI | YGGCCR | 1 cut(s) | 537 |
| AcsI | RAATTY | 1 cut(s) | 812 |
| AcuI | CTGAAG | 2 cut(s) | 162, 544 |
| AfaI | GTAC | 3 cut(s) | 406, 685, 835 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 515 |
| AflIII | ACRYGT | 1 cut(s) | 482 |
| AgsI | TTSAA | 7 cut(s) | 29, 671, 713, 749, 772, 867, 901 |
| AluBI | AGCT | 6 cut(s) | 182, 302, 591, 654, 741, 756 |
| AluI | AGCT | 6 cut(s) | 182, 302, 591, 654, 741, 756 |
| Alw26I | GTCTC | 3 cut(s) | 96, 147, 825 |
| AoxI | GGCC | 1 cut(s) | 537 |
| ApeKI | GCWGC | 2 cut(s) | 315, 435 |
| ApoI | RAATTY | 1 cut(s) | 812 |
| AspLEI | GCGC | 1 cut(s) | 942 |
| AsuC2I | CCSGG | 1 cut(s) | 225 |
| AsuHPI | GGTGA | 1 cut(s) | 486 |
| AsuNHI | GCTAGC | 1 cut(s) | 182 |
| BbsI | GAAGAC | 4 cut(s) | 49, 75, 123, 265 |
| BbvI | GCAGC | 2 cut(s) | 302, 422 |
| BccI | CCATC | 3 cut(s) | 103, 148, 389 |
| BclI | TGATCA | 2 cut(s) | 283, 568 |
| BcnI | CCSGG | 1 cut(s) | 225 |
| BcoDI | GTCTC | 3 cut(s) | 96, 147, 825 |
| BfaI | CTAG | 2 cut(s) | 183, 753 |
| BfmI | CTRYAG | 1 cut(s) | 845 |
| BfuAI | ACCTGC | 1 cut(s) | 838 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 360 |
| BisI | GCNGC | 2 cut(s) | 316, 436 |
| BlsI | GCNGC | 2 cut(s) | 317, 437 |
| BmcAI | AGTACT | 1 cut(s) | 835 |
| Bme1390I | CCNGG | 1 cut(s) | 225 |
| BmiI | GGNNCC | 1 cut(s) | 364 |
| BmrFI | CCNGG | 1 cut(s) | 225 |
| BmsI | GCATC | 1 cut(s) | 509 |
| BmtI | GCTAGC | 1 cut(s) | 186 |
| BpiI | GAAGAC | 4 cut(s) | 49, 75, 123, 265 |
| BpmI | CTGGAG | 1 cut(s) | 171 |
| BpuMI | CCSGG | 1 cut(s) | 225 |
| BsaI | GGTCTC | 2 cut(s) | 147, 825 |
| BsaJI | CCNNGG | 4 cut(s) | 47, 223, 534, 540 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 515 |
| Bse1I | ACTGG | 2 cut(s) | 277, 446 |
| BseDI | CCNNGG | 4 cut(s) | 47, 223, 534, 540 |
| BseGI | GGATG | 2 cut(s) | 118, 524 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 515 |
| BseMII | CTCAG | 2 cut(s) | 27, 87 |
| BseNI | ACTGG | 2 cut(s) | 277, 446 |
| BseXI | GCAGC | 2 cut(s) | 302, 422 |
| BsgI | GTGCAG | 1 cut(s) | 301 |
| BshFI | GGCC | 1 cut(s) | 539 |
| BsiSI | CCGG | 1 cut(s) | 225 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 515 |
| BsmAI | GTCTC | 3 cut(s) | 96, 147, 825 |
| BsmBI | CGTCTC | 1 cut(s) | 96 |
| BsnI | GGCC | 1 cut(s) | 539 |
| Bso31I | GGTCTC | 2 cut(s) | 147, 825 |
| Bsp143I | GATC | 3 cut(s) | 283, 568, 778 |
| BspACI | CCGC | 1 cut(s) | 65 |
| BspANI | GGCC | 1 cut(s) | 539 |
| BspCNI | CTCAG | 2 cut(s) | 26, 86 |
| BspLI | GGNNCC | 1 cut(s) | 364 |
| BspMAI | CTGCAG | 1 cut(s) | 849 |
| BspMI | ACCTGC | 1 cut(s) | 838 |
| BspOI | GCTAGC | 1 cut(s) | 186 |
| BspTNI | GGTCTC | 2 cut(s) | 147, 825 |
| BsrI | ACTGG | 2 cut(s) | 277, 446 |
| BssECI | CCNNGG | 4 cut(s) | 47, 223, 534, 540 |
| BssMI | GATC | 3 cut(s) | 283, 568, 778 |
| BssT1I | CCWWGG | 1 cut(s) | 540 |
| Bst4CI | ACNGT | 3 cut(s) | 265, 623, 736 |
| BstC8I | GCNNGC | 3 cut(s) | 184, 304, 845 |
| BstDEI | CTNAG | 2 cut(s) | 13, 73 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 513 |
| BstF5I | GGATG | 2 cut(s) | 118, 524 |
| BstHHI | GCGC | 1 cut(s) | 942 |
| BstKTI | GATC | 3 cut(s) | 286, 571, 781 |
| BstMAI | GTCTC | 3 cut(s) | 96, 147, 825 |
| BstMBI | GATC | 3 cut(s) | 283, 568, 778 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 192, 246, 312, 360 |
| BstNSI | RCATGY | 1 cut(s) | 486 |
| BstSCI | CCNGG | 1 cut(s) | 223 |
| BstSFI | CTRYAG | 1 cut(s) | 845 |
| BstV1I | GCAGC | 2 cut(s) | 302, 422 |
| BstV2I | GAAGAC | 4 cut(s) | 49, 75, 123, 265 |
| BsuRI | GGCC | 1 cut(s) | 539 |
| BtsCI | GGATG | 2 cut(s) | 118, 524 |
| BtsIMutI | CAGTG | 2 cut(s) | 284, 453 |
| BveI | ACCTGC | 1 cut(s) | 838 |
| Cac8I | GCNNGC | 3 cut(s) | 184, 304, 845 |
| CfoI | GCGC | 1 cut(s) | 942 |
| Csp6I | GTAC | 3 cut(s) | 405, 684, 834 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 889, 924 |
| CviAII | CATG | 5 cut(s) | 473, 483, 504, 760, 888 |
| CviQI | GTAC | 3 cut(s) | 405, 684, 834 |
| DdeI | CTNAG | 2 cut(s) | 13, 73 |
| DpnI | GATC | 3 cut(s) | 285, 570, 780 |
| DpnII | GATC | 3 cut(s) | 283, 568, 778 |
| EaeI | YGGCCR | 1 cut(s) | 537 |
| Eco130I | CCWWGG | 1 cut(s) | 540 |
| Eco31I | GGTCTC | 2 cut(s) | 147, 825 |
| Eco57I | CTGAAG | 2 cut(s) | 162, 544 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 513 |
| EcoT14I | CCWWGG | 1 cut(s) | 540 |
| ErhI | CCWWGG | 1 cut(s) | 540 |
| Esp3I | CGTCTC | 1 cut(s) | 96 |
| FaeI | CATG | 5 cut(s) | 476, 486, 507, 763, 891 |
| FatI | CATG | 5 cut(s) | 472, 482, 503, 759, 887 |
| FbaI | TGATCA | 2 cut(s) | 283, 568 |
| Fnu4HI | GCNGC | 2 cut(s) | 316, 436 |
| FokI | GGATG | 2 cut(s) | 125, 531 |
| Fsp4HI | GCNGC | 2 cut(s) | 316, 436 |
| FspBI | CTAG | 2 cut(s) | 183, 753 |
| GlaI | GCGC | 1 cut(s) | 941 |
| GluI | GCNGC | 2 cut(s) | 316, 436 |
| GsuI | CTGGAG | 1 cut(s) | 171 |
| HaeIII | GGCC | 1 cut(s) | 539 |
| HapII | CCGG | 1 cut(s) | 225 |
| HhaI | GCGC | 1 cut(s) | 942 |
| Hin1II | CATG | 5 cut(s) | 476, 486, 507, 763, 891 |
| Hin6I | GCGC | 1 cut(s) | 940 |
| HinP1I | GCGC | 1 cut(s) | 940 |
| HindIII | AAGCTT | 1 cut(s) | 589 |
| HinfI | GANTC | 4 cut(s) | 71, 95, 398, 578 |
| HpaII | CCGG | 1 cut(s) | 225 |
| HphI | GGTGA | 1 cut(s) | 486 |
| Hpy166II | GTNNAC | 2 cut(s) | 254, 494 |
| Hpy188I | TCNGA | 3 cut(s) | 16, 76, 825 |
| Hpy188III | TCNNGA | 5 cut(s) | 39, 99, 385, 867, 919 |
| Hpy8I | GTNNAC | 2 cut(s) | 254, 494 |
| HpyAV | CCTTC | 3 cut(s) | 71, 185, 873 |
| HpyCH4III | ACNGT | 3 cut(s) | 265, 623, 736 |
| HpyCH4V | TGCA | 4 cut(s) | 318, 438, 522, 847 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 192, 246, 312, 360 |
| HpyF3I | CTNAG | 2 cut(s) | 13, 73 |
| Hsp92II | CATG | 5 cut(s) | 476, 486, 507, 763, 891 |
| HspAI | GCGC | 1 cut(s) | 940 |
| Ksp22I | TGATCA | 2 cut(s) | 283, 568 |
| Kzo9I | GATC | 3 cut(s) | 283, 568, 778 |
| LmnI | GCTCC | 2 cut(s) | 311, 368 |
| Lsp1109I | GCAGC | 2 cut(s) | 302, 422 |
| LweI | GCATC | 1 cut(s) | 509 |
| MaeI | CTAG | 2 cut(s) | 183, 753 |
| MaeIII | GTNAC | 2 cut(s) | 448, 730 |
| MalI | GATC | 3 cut(s) | 285, 570, 780 |
| MboI | GATC | 3 cut(s) | 283, 568, 778 |
| MboII | GAAGA | 9 cut(s) | 41, 54, 80, 104, 128, 265, 400, 475, 773 |
| MluCI | AATT | 3 cut(s) | 243, 630, 812 |
| MlyI | GAGTC | 1 cut(s) | 65 |
| MnlI | CCTC | 4 cut(s) | 57, 544, 707, 757 |
| MseI | TTAA | 3 cut(s) | 215, 852, 881 |
| MslI | CAYNNNNRTG | 1 cut(s) | 487 |
| MspI | CCGG | 1 cut(s) | 225 |
| MspR9I | CCNGG | 1 cut(s) | 225 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 192, 246, 312, 360 |
| NciI | CCSGG | 1 cut(s) | 225 |
| NdeII | GATC | 3 cut(s) | 283, 568, 778 |
| NheI | GCTAGC | 1 cut(s) | 182 |
| NlaIII | CATG | 5 cut(s) | 476, 486, 507, 763, 891 |
| NlaIV | GGNNCC | 1 cut(s) | 364 |
| NmeAIII | GCCGAG | 2 cut(s) | 401, 515 |
| NmuCI | GTSAC | 2 cut(s) | 448, 730 |
| NspI | RCATGY | 1 cut(s) | 486 |
| PciI | ACATGT | 1 cut(s) | 482 |
| PfeI | GAWTC | 3 cut(s) | 95, 398, 578 |
| PkrI | GCNGC | 2 cut(s) | 317, 437 |
| PleI | GAGTC | 1 cut(s) | 65 |
| PpsI | GAGTC | 1 cut(s) | 65 |
| PscI | ACATGT | 1 cut(s) | 482 |
| PspN4I | GGNNCC | 1 cut(s) | 364 |
| PstI | CTGCAG | 1 cut(s) | 849 |
| RsaI | GTAC | 3 cut(s) | 406, 685, 835 |
| RsaNI | GTAC | 3 cut(s) | 405, 684, 834 |
| RseI | CAYNNNNRTG | 1 cut(s) | 487 |
| SaqAI | TTAA | 3 cut(s) | 215, 852, 881 |
| SatI | GCNGC | 2 cut(s) | 316, 436 |
| Sau3AI | GATC | 3 cut(s) | 283, 568, 778 |
| SbfI | CCTGCAGG | 1 cut(s) | 849 |
| ScaI | AGTACT | 1 cut(s) | 835 |
| SchI | GAGTC | 1 cut(s) | 65 |
| ScrFI | CCNGG | 1 cut(s) | 225 |
| SdaI | CCTGCAGG | 1 cut(s) | 849 |
| SfaNI | GCATC | 1 cut(s) | 509 |
| SfcI | CTRYAG | 1 cut(s) | 845 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 487 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 849 |
| Sse9I | AATT | 3 cut(s) | 243, 630, 812 |
| SsiI | CCGC | 1 cut(s) | 65 |
| SspI | AATATT | 2 cut(s) | 202, 442 |
| SspMI | CTAG | 2 cut(s) | 183, 753 |
| StyD4I | CCNGG | 1 cut(s) | 223 |
| StyI | CCWWGG | 1 cut(s) | 540 |
| TaaI | ACNGT | 3 cut(s) | 265, 623, 736 |
| TaqI | TCGA | 4 cut(s) | 5, 173, 384, 412 |
| TaqII | GACCGA | 1 cut(s) | 807 |
| TasI | AATT | 3 cut(s) | 243, 630, 812 |
| TatI | WGTACW | 2 cut(s) | 404, 833 |
| TfiI | GAWTC | 3 cut(s) | 95, 398, 578 |
| Tru1I | TTAA | 3 cut(s) | 215, 852, 881 |
| Tru9I | TTAA | 3 cut(s) | 215, 852, 881 |
| TscAI | CASTG | 2 cut(s) | 284, 453 |
| TseFI | GTSAC | 2 cut(s) | 448, 730 |
| TseI | GCWGC | 2 cut(s) | 315, 435 |
| Tsp45I | GTSAC | 2 cut(s) | 448, 730 |
| TspDTI | ATGAA | 6 cut(s) | 105, 129, 483, 489, 492, 748 |
| TspGWI | ACGGA | 1 cut(s) | 13 |
| TspRI | CASTG | 2 cut(s) | 284, 453 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 513 |
| XapI | RAATTY | 1 cut(s) | 812 |
| XceI | RCATGY | 1 cut(s) | 486 |
| XspI | CTAG | 2 cut(s) | 183, 753 |
| ZrmI | AGTACT | 1 cut(s) | 835 |