E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 492, 989 |
| AatII | GACGTC | 1 cut(s) | 70 |
| AciI | CCGC | 1 cut(s) | 21 |
| AcoI | YGGCCR | 2 cut(s) | 99, 1018 |
| AcsI | RAATTY | 3 cut(s) | 236, 764, 825 |
| AcuI | CTGAAG | 1 cut(s) | 246 |
| AcyI | GRCGYC | 1 cut(s) | 67 |
| AfaI | GTAC | 2 cut(s) | 725, 946 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 324, 641 |
| AgsI | TTSAA | 5 cut(s) | 663, 703, 741, 779, 860 |
| AjiI | CACGTC | 1 cut(s) | 579 |
| AjnI | CCWGG | 3 cut(s) | 53, 476, 994 |
| AluBI | AGCT | 5 cut(s) | 89, 293, 428, 735, 1024 |
| AluI | AGCT | 5 cut(s) | 89, 293, 428, 735, 1024 |
| Alw26I | GTCTC | 3 cut(s) | 58, 293, 661 |
| Ama87I | CYCGRG | 1 cut(s) | 248 |
| AoxI | GGCC | 3 cut(s) | 99, 479, 1018 |
| ApeKI | GCWGC | 6 cut(s) | 48, 89, 290, 441, 645, 732 |
| ApoI | RAATTY | 3 cut(s) | 236, 764, 825 |
| AspS9I | GGNCC | 3 cut(s) | 41, 261, 480 |
| AsuHPI | GGTGA | 5 cut(s) | 242, 331, 612, 853, 1010 |
| AvaI | CYCGRG | 1 cut(s) | 248 |
| AvaII | GGWCC | 2 cut(s) | 41, 261 |
| BalI | TGGCCA | 2 cut(s) | 101, 1020 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 659, 691 |
| BbsI | GAAGAC | 1 cut(s) | 394 |
| BbvI | GCAGC | 6 cut(s) | 60, 76, 302, 453, 632, 744 |
| BccI | CCATC | 5 cut(s) | 26, 344, 785, 941, 1024 |
| BciT130I | CCWGG | 3 cut(s) | 55, 478, 996 |
| BcoDI | GTCTC | 3 cut(s) | 58, 293, 661 |
| BfaI | CTAG | 3 cut(s) | 521, 830, 864 |
| BfmI | CTRYAG | 2 cut(s) | 128, 439 |
| BisI | GCNGC | 6 cut(s) | 49, 90, 291, 442, 646, 733 |
| BlsI | GCNGC | 6 cut(s) | 50, 91, 292, 443, 647, 734 |
| BmcAI | AGTACT | 1 cut(s) | 946 |
| Bme1390I | CCNGG | 3 cut(s) | 55, 478, 996 |
| Bme18I | GGWCC | 2 cut(s) | 41, 261 |
| BmeT110I | CYCGRG | 1 cut(s) | 248 |
| BmgBI | CACGTC | 1 cut(s) | 579 |
| BmgT120I | GGNCC | 3 cut(s) | 41, 261, 480 |
| BmrFI | CCNGG | 3 cut(s) | 55, 478, 996 |
| BmsI | GCATC | 1 cut(s) | 276 |
| BpiI | GAAGAC | 1 cut(s) | 394 |
| BpuEI | CTTGAG | 2 cut(s) | 279, 1010 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1034 |
| BsaHI | GRCGYC | 1 cut(s) | 67 |
| BsaJI | CCNNGG | 6 cut(s) | 53, 257, 285, 318, 906, 994 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 324, 641 |
| Bse1I | ACTGG | 1 cut(s) | 406 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1034 |
| BseBI | CCWGG | 3 cut(s) | 55, 478, 996 |
| BseDI | CCNNGG | 6 cut(s) | 53, 257, 285, 318, 906, 994 |
| BseGI | GGATG | 2 cut(s) | 579, 917 |
| BseJI | GATNNNNATC | 1 cut(s) | 1034 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 324, 641 |
| BseMII | CTCAG | 1 cut(s) | 934 |
| BseNI | ACTGG | 1 cut(s) | 406 |
| BseRI | GAGGAG | 5 cut(s) | 129, 132, 135, 179, 424 |
| BseXI | GCAGC | 6 cut(s) | 60, 76, 302, 453, 632, 744 |
| BshFI | GGCC | 3 cut(s) | 101, 481, 1020 |
| BsiHKCI | CYCGRG | 1 cut(s) | 248 |
| BslFI | GGGAC | 2 cut(s) | 354, 380 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 324, 641 |
| BsmAI | GTCTC | 3 cut(s) | 58, 293, 661 |
| BsmBI | CGTCTC | 1 cut(s) | 58 |
| BsmFI | GGGAC | 2 cut(s) | 354, 380 |
| BsmI | GAATGC | 1 cut(s) | 616 |
| BsnI | GGCC | 3 cut(s) | 101, 481, 1020 |
| BsoBI | CYCGRG | 1 cut(s) | 248 |
| Bsp1407I | TGTACA | 1 cut(s) | 723 |
| Bsp143I | GATC | 1 cut(s) | 889 |
| BspACI | CCGC | 1 cut(s) | 21 |
| BspANI | GGCC | 3 cut(s) | 101, 481, 1020 |
| BspCNI | CTCAG | 1 cut(s) | 933 |
| BspMAI | CTGCAG | 1 cut(s) | 443 |
| BsrGI | TGTACA | 1 cut(s) | 723 |
| BsrI | ACTGG | 1 cut(s) | 406 |
| BssECI | CCNNGG | 6 cut(s) | 53, 257, 285, 318, 906, 994 |
| BssMI | GATC | 1 cut(s) | 889 |
| BssNI | GRCGYC | 1 cut(s) | 67 |
| BssT1I | CCWWGG | 1 cut(s) | 906 |
| Bst2UI | CCWGG | 3 cut(s) | 55, 478, 996 |
| Bst4CI | ACNGT | 4 cut(s) | 97, 394, 930, 944 |
| Bst6I | CTCTTC | 3 cut(s) | 56, 156, 664 |
| BstACI | GRCGYC | 1 cut(s) | 67 |
| BstAUI | TGTACA | 1 cut(s) | 723 |
| BstC8I | GCNNGC | 2 cut(s) | 430, 1022 |
| BstDEI | CTNAG | 2 cut(s) | 848, 920 |
| BstDSI | CCRYGG | 1 cut(s) | 318 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 639 |
| BstF5I | GGATG | 2 cut(s) | 579, 917 |
| BstKTI | GATC | 1 cut(s) | 892 |
| BstMAI | GTCTC | 3 cut(s) | 58, 293, 661 |
| BstMBI | GATC | 1 cut(s) | 889 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 98, 290, 438 |
| BstNI | CCWGG | 3 cut(s) | 55, 478, 996 |
| BstNSI | RCATGY | 1 cut(s) | 612 |
| BstSCI | CCNGG | 3 cut(s) | 53, 476, 994 |
| BstSFI | CTRYAG | 2 cut(s) | 128, 439 |
| BstV1I | GCAGC | 6 cut(s) | 60, 76, 302, 453, 632, 744 |
| BstV2I | GAAGAC | 1 cut(s) | 394 |
| BsuRI | GGCC | 3 cut(s) | 101, 481, 1020 |
| BtgI | CCRYGG | 1 cut(s) | 318 |
| BtrI | CACGTC | 1 cut(s) | 579 |
| BtsCI | GGATG | 2 cut(s) | 579, 917 |
| BtsIMutI | CAGTG | 1 cut(s) | 413 |
| Cac8I | GCNNGC | 2 cut(s) | 430, 1022 |
| Cfr13I | GGNCC | 3 cut(s) | 41, 261, 480 |
| Csp6I | GTAC | 2 cut(s) | 724, 945 |
| CviAII | CATG | 5 cut(s) | 471, 540, 599, 609, 630 |
| CviQI | GTAC | 2 cut(s) | 724, 945 |
| DdeI | CTNAG | 2 cut(s) | 848, 920 |
| DpnI | GATC | 1 cut(s) | 891 |
| DpnII | GATC | 1 cut(s) | 889 |
| EaeI | YGGCCR | 2 cut(s) | 99, 1018 |
| Eam1104I | CTCTTC | 3 cut(s) | 56, 156, 664 |
| EarI | CTCTTC | 3 cut(s) | 56, 156, 664 |
| EciI | GGCGGA | 1 cut(s) | 36 |
| Eco130I | CCWWGG | 1 cut(s) | 906 |
| Eco32I | GATATC | 1 cut(s) | 1036 |
| Eco47I | GGWCC | 2 cut(s) | 41, 261 |
| Eco57I | CTGAAG | 1 cut(s) | 246 |
| Eco88I | CYCGRG | 1 cut(s) | 248 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 639 |
| EcoRI | GAATTC | 1 cut(s) | 236 |
| EcoRII | CCWGG | 3 cut(s) | 53, 476, 994 |
| EcoRV | GATATC | 1 cut(s) | 1036 |
| EcoT14I | CCWWGG | 1 cut(s) | 906 |
| EcoT22I | ATGCAT | 1 cut(s) | 618 |
| ErhI | CCWWGG | 1 cut(s) | 906 |
| Esp3I | CGTCTC | 1 cut(s) | 58 |
| FaeI | CATG | 5 cut(s) | 474, 543, 602, 612, 633 |
| FaqI | GGGAC | 2 cut(s) | 354, 380 |
| FatI | CATG | 5 cut(s) | 470, 539, 598, 608, 629 |
| FauNDI | CATATG | 1 cut(s) | 1043 |
| Fnu4HI | GCNGC | 6 cut(s) | 49, 90, 291, 442, 646, 733 |
| FokI | GGATG | 2 cut(s) | 586, 924 |
| Fsp4HI | GCNGC | 6 cut(s) | 49, 90, 291, 442, 646, 733 |
| FspBI | CTAG | 3 cut(s) | 521, 830, 864 |
| GluI | GCNGC | 6 cut(s) | 49, 90, 291, 442, 646, 733 |
| HaeIII | GGCC | 3 cut(s) | 101, 481, 1020 |
| Hin1I | GRCGYC | 1 cut(s) | 67 |
| Hin1II | CATG | 5 cut(s) | 474, 543, 602, 612, 633 |
| HinfI | GANTC | 6 cut(s) | 9, 146, 200, 251, 383, 524 |
| HphI | GGTGA | 5 cut(s) | 242, 331, 612, 853, 1010 |
| Hpy166II | GTNNAC | 1 cut(s) | 917 |
| Hpy188I | TCNGA | 4 cut(s) | 84, 157, 205, 923 |
| Hpy188III | TCNNGA | 4 cut(s) | 296, 521, 779, 1027 |
| Hpy8I | GTNNAC | 1 cut(s) | 917 |
| Hpy99I | CGWCG | 1 cut(s) | 72 |
| HpyAV | CCTTC | 4 cut(s) | 32, 221, 477, 1081 |
| HpyCH4III | ACNGT | 4 cut(s) | 97, 394, 930, 944 |
| HpyCH4IV | ACGT | 2 cut(s) | 67, 578 |
| HpyCH4V | TGCA | 7 cut(s) | 441, 564, 582, 616, 648, 710, 1041 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 98, 290, 438 |
| HpyF3I | CTNAG | 2 cut(s) | 848, 920 |
| HpySE526I | ACGT | 2 cut(s) | 67, 578 |
| Hsp92I | GRCGYC | 1 cut(s) | 67 |
| Hsp92II | CATG | 5 cut(s) | 474, 543, 602, 612, 633 |
| Kzo9I | GATC | 1 cut(s) | 889 |
| LmnI | GCTCC | 2 cut(s) | 437, 986 |
| Lsp1109I | GCAGC | 6 cut(s) | 60, 76, 302, 453, 632, 744 |
| LweI | GCATC | 1 cut(s) | 276 |
| MaeI | CTAG | 3 cut(s) | 521, 830, 864 |
| MaeII | ACGT | 2 cut(s) | 67, 578 |
| MaeIII | GTNAC | 2 cut(s) | 241, 1087 |
| MalI | GATC | 1 cut(s) | 891 |
| MboI | GATC | 1 cut(s) | 889 |
| MlsI | TGGCCA | 2 cut(s) | 101, 1020 |
| MluCI | AATT | 6 cut(s) | 236, 272, 528, 741, 764, 825 |
| MluNI | TGGCCA | 2 cut(s) | 101, 1020 |
| MlyI | GAGTC | 1 cut(s) | 260 |
| Mox20I | TGGCCA | 2 cut(s) | 101, 1020 |
| Mph1103I | ATGCAT | 1 cut(s) | 618 |
| MscI | TGGCCA | 2 cut(s) | 101, 1020 |
| MseI | TTAA | 3 cut(s) | 453, 756, 1093 |
| Msp20I | TGGCCA | 2 cut(s) | 101, 1020 |
| MspA1I | CMGCKG | 1 cut(s) | 89 |
| MspR9I | CCNGG | 3 cut(s) | 55, 478, 996 |
| Mva1269I | GAATGC | 1 cut(s) | 616 |
| MvaI | CCWGG | 3 cut(s) | 55, 478, 996 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 98, 290, 438 |
| NdeI | CATATG | 1 cut(s) | 1043 |
| NdeII | GATC | 1 cut(s) | 889 |
| NlaIII | CATG | 5 cut(s) | 474, 543, 602, 612, 633 |
| NmeAIII | GCCGAG | 2 cut(s) | 266, 282 |
| NsiI | ATGCAT | 1 cut(s) | 618 |
| NspI | RCATGY | 1 cut(s) | 612 |
| PaeR7I | CTCGAG | 1 cut(s) | 248 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 11, 246 |
| PctI | GAATGC | 1 cut(s) | 616 |
| PfeI | GAWTC | 5 cut(s) | 9, 146, 200, 383, 524 |
| PflFI | GACNNNGTC | 1 cut(s) | 69 |
| PkrI | GCNGC | 6 cut(s) | 50, 91, 292, 443, 647, 734 |
| PleI | GAGTC | 1 cut(s) | 259 |
| PpsI | GAGTC | 1 cut(s) | 259 |
| PsiI | TTATAA | 2 cut(s) | 492, 989 |
| Psp6I | CCWGG | 3 cut(s) | 53, 476, 994 |
| PspGI | CCWGG | 3 cut(s) | 53, 476, 994 |
| PspPI | GGNCC | 3 cut(s) | 41, 261, 480 |
| PspXI | VCTCGAGB | 1 cut(s) | 248 |
| PstI | CTGCAG | 1 cut(s) | 443 |
| PsyI | GACNNNGTC | 1 cut(s) | 69 |
| PvuII | CAGCTG | 1 cut(s) | 89 |
| RsaI | GTAC | 2 cut(s) | 725, 946 |
| RsaNI | GTAC | 2 cut(s) | 724, 945 |
| SaqAI | TTAA | 3 cut(s) | 453, 756, 1093 |
| SatI | GCNGC | 6 cut(s) | 49, 90, 291, 442, 646, 733 |
| Sau3AI | GATC | 1 cut(s) | 889 |
| Sau96I | GGNCC | 3 cut(s) | 41, 261, 480 |
| ScaI | AGTACT | 1 cut(s) | 946 |
| SchI | GAGTC | 1 cut(s) | 260 |
| ScrFI | CCNGG | 3 cut(s) | 55, 478, 996 |
| SfaNI | GCATC | 1 cut(s) | 276 |
| SfcI | CTRYAG | 2 cut(s) | 128, 439 |
| Sfr274I | CTCGAG | 1 cut(s) | 248 |
| SinI | GGWCC | 2 cut(s) | 41, 261 |
| SlaI | CTCGAG | 1 cut(s) | 248 |
| SmlI | CTYRAG | 3 cut(s) | 248, 294, 1025 |
| SmoI | CTYRAG | 3 cut(s) | 248, 294, 1025 |
| Sse9I | AATT | 6 cut(s) | 236, 272, 528, 741, 764, 825 |
| SsiI | CCGC | 1 cut(s) | 21 |
| SspMI | CTAG | 3 cut(s) | 521, 830, 864 |
| StyD4I | CCNGG | 3 cut(s) | 53, 476, 994 |
| StyI | CCWWGG | 1 cut(s) | 906 |
| TaaI | ACNGT | 4 cut(s) | 97, 394, 930, 944 |
| TaiI | ACGT | 2 cut(s) | 70, 581 |
| TaqI | TCGA | 2 cut(s) | 249, 270 |
| TaqII | GACCGA | 2 cut(s) | 58, 362 |
| TasI | AATT | 6 cut(s) | 236, 272, 528, 741, 764, 825 |
| TatI | WGTACW | 2 cut(s) | 723, 944 |
| TfiI | GAWTC | 5 cut(s) | 9, 146, 200, 383, 524 |
| Tru1I | TTAA | 3 cut(s) | 453, 756, 1093 |
| Tru9I | TTAA | 3 cut(s) | 453, 756, 1093 |
| TscAI | CASTG | 1 cut(s) | 413 |
| TseI | GCWGC | 6 cut(s) | 48, 89, 290, 441, 645, 732 |
| TspDTI | ATGAA | 7 cut(s) | 42, 249, 459, 609, 615, 618, 963 |
| TspGWI | ACGGA | 3 cut(s) | 335, 586, 1056 |
| TspRI | CASTG | 1 cut(s) | 413 |
| Tth111I | GACNNNGTC | 1 cut(s) | 69 |
| VpaK11BI | GGWCC | 2 cut(s) | 41, 261 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 639 |
| XapI | RAATTY | 3 cut(s) | 236, 764, 825 |
| XbaI | TCTAGA | 1 cut(s) | 520 |
| XceI | RCATGY | 1 cut(s) | 612 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 271, 413 |
| XhoI | CTCGAG | 1 cut(s) | 248 |
| XspI | CTAG | 3 cut(s) | 521, 830, 864 |
| ZraI | GACGTC | 1 cut(s) | 68 |
| ZrmI | AGTACT | 1 cut(s) | 946 |
| Zsp2I | ATGCAT | 1 cut(s) | 618 |