E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 649 |
| Acc36I | ACCTGC | 1 cut(s) | 649 |
| AccBSI | CCGCTC | 1 cut(s) | 148 |
| AciI | CCGC | 3 cut(s) | 23, 59, 146 |
| AclWI | GGATC | 4 cut(s) | 117, 959, 972, 1017 |
| AcsI | RAATTY | 5 cut(s) | 650, 742, 988, 1014, 1033 |
| AcuI | CTGAAG | 1 cut(s) | 630 |
| AfaI | GTAC | 3 cut(s) | 478, 890, 996 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 233, 495 |
| AgsI | TTSAA | 5 cut(s) | 595, 655, 737, 814, 835 |
| AjnI | CCWGG | 2 cut(s) | 226, 232 |
| AluBI | AGCT | 3 cut(s) | 51, 817, 859 |
| AluI | AGCT | 3 cut(s) | 51, 817, 859 |
| Alw26I | GTCTC | 2 cut(s) | 346, 981 |
| AlwI | GGATC | 4 cut(s) | 117, 959, 972, 1017 |
| ApeKI | GCWGC | 1 cut(s) | 327 |
| ApoI | RAATTY | 5 cut(s) | 650, 742, 988, 1014, 1033 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 171, 203 |
| AspS9I | GGNCC | 1 cut(s) | 230 |
| AsuC2I | CCSGG | 1 cut(s) | 876 |
| AsuHPI | GGTGA | 4 cut(s) | 11, 47, 705, 809 |
| AvaII | GGWCC | 1 cut(s) | 230 |
| BaeGI | GKGCMC | 1 cut(s) | 227 |
| BamHI | GGATCC | 1 cut(s) | 964 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 714, 746 |
| BbvI | GCAGC | 1 cut(s) | 314 |
| BccI | CCATC | 2 cut(s) | 170, 471 |
| BciT130I | CCWGG | 2 cut(s) | 228, 234 |
| BcnI | CCSGG | 1 cut(s) | 876 |
| BcoDI | GTCTC | 2 cut(s) | 346, 981 |
| BfuAI | ACCTGC | 1 cut(s) | 649 |
| BisI | GCNGC | 2 cut(s) | 146, 328 |
| BlsI | GCNGC | 2 cut(s) | 147, 329 |
| Bme1390I | CCNGG | 3 cut(s) | 228, 234, 876 |
| Bme18I | GGWCC | 1 cut(s) | 230 |
| BmgT120I | GGNCC | 1 cut(s) | 230 |
| BmiI | GGNNCC | 2 cut(s) | 873, 966 |
| BmrFI | CCNGG | 3 cut(s) | 228, 234, 876 |
| BmsI | GCATC | 3 cut(s) | 254, 639, 831 |
| Bpu10I | CCTNAGC | 1 cut(s) | 945 |
| BpuMI | CCSGG | 1 cut(s) | 876 |
| BsaJI | CCNNGG | 2 cut(s) | 226, 233 |
| BsaWI | WCCGGW | 1 cut(s) | 130 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 233, 495 |
| Bse1I | ACTGG | 1 cut(s) | 427 |
| Bse3DI | GCAATG | 2 cut(s) | 279, 939 |
| BseBI | CCWGG | 2 cut(s) | 228, 234 |
| BseDI | CCNNGG | 2 cut(s) | 226, 233 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 233, 495 |
| BseMI | GCAATG | 2 cut(s) | 279, 939 |
| BseMII | CTCAG | 2 cut(s) | 309, 959 |
| BseNI | ACTGG | 1 cut(s) | 427 |
| BseSI | GKGCMC | 1 cut(s) | 227 |
| BseXI | GCAGC | 1 cut(s) | 314 |
| BsgI | GTGCAG | 1 cut(s) | 313 |
| Bsh1285I | CGRYCG | 2 cut(s) | 95, 483 |
| BsiEI | CGRYCG | 2 cut(s) | 95, 483 |
| BsiSI | CCGG | 2 cut(s) | 131, 876 |
| BslFI | GGGAC | 1 cut(s) | 761 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 233, 495 |
| BsmAI | GTCTC | 2 cut(s) | 346, 981 |
| BsmBI | CGTCTC | 1 cut(s) | 981 |
| BsmFI | GGGAC | 1 cut(s) | 761 |
| BsmI | GAATGC | 2 cut(s) | 380, 416 |
| Bsp1286I | GDGCHC | 1 cut(s) | 227 |
| Bsp1407I | TGTACA | 1 cut(s) | 888 |
| Bsp143I | GATC | 5 cut(s) | 92, 109, 480, 964, 1009 |
| BspACI | CCGC | 3 cut(s) | 23, 59, 146 |
| BspCNI | CTCAG | 2 cut(s) | 310, 958 |
| BspLI | GGNNCC | 2 cut(s) | 873, 966 |
| BspMI | ACCTGC | 1 cut(s) | 649 |
| BspPI | GGATC | 4 cut(s) | 117, 959, 972, 1017 |
| BsrBI | CCGCTC | 1 cut(s) | 148 |
| BsrDI | GCAATG | 2 cut(s) | 279, 939 |
| BsrGI | TGTACA | 1 cut(s) | 888 |
| BsrI | ACTGG | 1 cut(s) | 427 |
| BssECI | CCNNGG | 2 cut(s) | 226, 233 |
| BssMI | GATC | 5 cut(s) | 92, 109, 480, 964, 1009 |
| Bst2UI | CCWGG | 2 cut(s) | 228, 234 |
| Bst4CI | ACNGT | 7 cut(s) | 128, 446, 511, 622, 764, 785, 955 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 198, 641 |
| BstAUI | TGTACA | 1 cut(s) | 888 |
| BstC8I | GCNNGC | 1 cut(s) | 915 |
| BstDEI | CTNAG | 3 cut(s) | 99, 318, 945 |
| BstKTI | GATC | 5 cut(s) | 95, 112, 483, 967, 1012 |
| BstMAI | GTCTC | 2 cut(s) | 346, 981 |
| BstMBI | GATC | 5 cut(s) | 92, 109, 480, 964, 1009 |
| BstMCI | CGRYCG | 2 cut(s) | 95, 483 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 67, 198, 641 |
| BstNI | CCWGG | 2 cut(s) | 228, 234 |
| BstSCI | CCNGG | 3 cut(s) | 226, 232, 874 |
| BstSLI | GKGCMC | 1 cut(s) | 227 |
| BstV1I | GCAGC | 1 cut(s) | 314 |
| BstX2I | RGATCY | 2 cut(s) | 109, 964 |
| BstYI | RGATCY | 2 cut(s) | 109, 964 |
| BtgZI | GCGATG | 2 cut(s) | 277, 616 |
| BtsIMutI | CAGTG | 4 cut(s) | 173, 420, 507, 593 |
| BveI | ACCTGC | 1 cut(s) | 649 |
| Cac8I | GCNNGC | 1 cut(s) | 915 |
| Cfr13I | GGNCC | 1 cut(s) | 230 |
| CseI | GACGC | 2 cut(s) | 446, 636 |
| Csp6I | GTAC | 3 cut(s) | 477, 889, 995 |
| CviAII | CATG | 4 cut(s) | 306, 605, 769, 794 |
| CviQI | GTAC | 3 cut(s) | 477, 889, 995 |
| DdeI | CTNAG | 3 cut(s) | 99, 318, 945 |
| DpnI | GATC | 5 cut(s) | 94, 111, 482, 966, 1011 |
| DpnII | GATC | 5 cut(s) | 92, 109, 480, 964, 1009 |
| Eco47I | GGWCC | 1 cut(s) | 230 |
| Eco57I | CTGAAG | 1 cut(s) | 630 |
| EcoRI | GAATTC | 3 cut(s) | 650, 742, 988 |
| EcoRII | CCWGG | 2 cut(s) | 226, 232 |
| Esp3I | CGTCTC | 1 cut(s) | 981 |
| FaeI | CATG | 4 cut(s) | 309, 608, 772, 797 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 251, 283, 328, 360 |
| FaqI | GGGAC | 1 cut(s) | 761 |
| FatI | CATG | 4 cut(s) | 305, 604, 768, 793 |
| FauI | CCCGC | 2 cut(s) | 30, 66 |
| Fnu4HI | GCNGC | 2 cut(s) | 146, 328 |
| Fsp4HI | GCNGC | 2 cut(s) | 146, 328 |
| GluI | GCNGC | 2 cut(s) | 146, 328 |
| HapII | CCGG | 2 cut(s) | 131, 876 |
| HgaI | GACGC | 2 cut(s) | 446, 636 |
| Hin1II | CATG | 4 cut(s) | 309, 608, 772, 797 |
| HindIII | AAGCTT | 2 cut(s) | 815, 857 |
| HinfI | GANTC | 3 cut(s) | 290, 557, 710 |
| HpaII | CCGG | 2 cut(s) | 131, 876 |
| HphI | GGTGA | 4 cut(s) | 11, 47, 705, 809 |
| Hpy166II | GTNNAC | 1 cut(s) | 788 |
| Hpy188I | TCNGA | 3 cut(s) | 567, 649, 1020 |
| Hpy188III | TCNNGA | 9 cut(s) | 113, 122, 287, 365, 655, 734, 826, 947, 1004 |
| Hpy8I | GTNNAC | 1 cut(s) | 788 |
| Hpy99I | CGWCG | 2 cut(s) | 462, 692 |
| HpyCH4III | ACNGT | 7 cut(s) | 128, 446, 511, 622, 764, 785, 955 |
| HpyCH4IV | ACGT | 2 cut(s) | 73, 974 |
| HpyCH4V | TGCA | 5 cut(s) | 192, 330, 378, 414, 793 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 67, 198, 641 |
| HpyF3I | CTNAG | 3 cut(s) | 99, 318, 945 |
| HpySE526I | ACGT | 2 cut(s) | 73, 974 |
| Hsp92II | CATG | 4 cut(s) | 309, 608, 772, 797 |
| Kzo9I | GATC | 5 cut(s) | 92, 109, 480, 964, 1009 |
| LmnI | GCTCC | 1 cut(s) | 598 |
| Lsp1109I | GCAGC | 1 cut(s) | 314 |
| LweI | GCATC | 3 cut(s) | 254, 639, 831 |
| MaeII | ACGT | 2 cut(s) | 73, 974 |
| MaeIII | GTNAC | 6 cut(s) | 215, 362, 446, 616, 700, 934 |
| MalI | GATC | 5 cut(s) | 94, 111, 482, 966, 1011 |
| MbiI | CCGCTC | 1 cut(s) | 148 |
| MboI | GATC | 5 cut(s) | 92, 109, 480, 964, 1009 |
| MflI | RGATCY | 2 cut(s) | 109, 964 |
| MhlI | GDGCHC | 1 cut(s) | 227 |
| MluCI | AATT | 8 cut(s) | 193, 415, 650, 742, 988, 999, 1014, 1033 |
| MmeI | TCCRAC | 3 cut(s) | 87, 786, 995 |
| MnlI | CCTC | 8 cut(s) | 273, 416, 499, 720, 916, 955, 978, 1000 |
| MseI | TTAA | 2 cut(s) | 905, 984 |
| MspI | CCGG | 2 cut(s) | 131, 876 |
| MspR9I | CCNGG | 3 cut(s) | 228, 234, 876 |
| Mva1269I | GAATGC | 2 cut(s) | 380, 416 |
| MvaI | CCWGG | 2 cut(s) | 228, 234 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 67, 198, 641 |
| NciI | CCSGG | 1 cut(s) | 876 |
| NdeII | GATC | 5 cut(s) | 92, 109, 480, 964, 1009 |
| NlaIII | CATG | 4 cut(s) | 309, 608, 772, 797 |
| NlaIV | GGNNCC | 2 cut(s) | 873, 966 |
| NmuCI | GTSAC | 2 cut(s) | 362, 446 |
| PaqCI | CACCTGC | 1 cut(s) | 649 |
| PctI | GAATGC | 2 cut(s) | 380, 416 |
| PfeI | GAWTC | 3 cut(s) | 290, 557, 710 |
| PkrI | GCNGC | 2 cut(s) | 147, 329 |
| Ple19I | CGATCG | 2 cut(s) | 95, 483 |
| Psp6I | CCWGG | 2 cut(s) | 226, 232 |
| PspGI | CCWGG | 2 cut(s) | 226, 232 |
| PspN4I | GGNNCC | 2 cut(s) | 873, 966 |
| PspPI | GGNCC | 1 cut(s) | 230 |
| PsuI | RGATCY | 2 cut(s) | 109, 964 |
| PvuI | CGATCG | 2 cut(s) | 95, 483 |
| RsaI | GTAC | 3 cut(s) | 478, 890, 996 |
| RsaNI | GTAC | 3 cut(s) | 477, 889, 995 |
| SaqAI | TTAA | 2 cut(s) | 905, 984 |
| SatI | GCNGC | 2 cut(s) | 146, 328 |
| Sau3AI | GATC | 5 cut(s) | 92, 109, 480, 964, 1009 |
| Sau96I | GGNCC | 1 cut(s) | 230 |
| ScrFI | CCNGG | 3 cut(s) | 228, 234, 876 |
| SduI | GDGCHC | 1 cut(s) | 227 |
| SfaNI | GCATC | 3 cut(s) | 254, 639, 831 |
| SinI | GGWCC | 1 cut(s) | 230 |
| Sse9I | AATT | 8 cut(s) | 193, 415, 650, 742, 988, 999, 1014, 1033 |
| SsiI | CCGC | 3 cut(s) | 23, 59, 146 |
| StyD4I | CCNGG | 3 cut(s) | 226, 232, 874 |
| TaaI | ACNGT | 7 cut(s) | 128, 446, 511, 622, 764, 785, 955 |
| TaiI | ACGT | 2 cut(s) | 76, 977 |
| TaqI | TCGA | 5 cut(s) | 141, 288, 408, 687, 1003 |
| TasI | AATT | 8 cut(s) | 193, 415, 650, 742, 988, 999, 1014, 1033 |
| TatI | WGTACW | 1 cut(s) | 888 |
| TauI | GCSGC | 1 cut(s) | 148 |
| TfiI | GAWTC | 3 cut(s) | 290, 557, 710 |
| Tru1I | TTAA | 2 cut(s) | 905, 984 |
| Tru9I | TTAA | 2 cut(s) | 905, 984 |
| TscAI | CASTG | 4 cut(s) | 173, 427, 514, 593 |
| TseFI | GTSAC | 2 cut(s) | 362, 446 |
| TseI | GCWGC | 1 cut(s) | 327 |
| Tsp45I | GTSAC | 2 cut(s) | 362, 446 |
| TspDTI | ATGAA | 3 cut(s) | 99, 363, 757 |
| TspGWI | ACGGA | 1 cut(s) | 705 |
| TspRI | CASTG | 4 cut(s) | 173, 427, 514, 593 |
| VpaK11BI | GGWCC | 1 cut(s) | 230 |
| XapI | RAATTY | 5 cut(s) | 650, 742, 988, 1014, 1033 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 860 |