E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 870 |
| AciI | CCGC | 2 cut(s) | 581, 642 |
| AclI | AACGTT | 1 cut(s) | 509 |
| AclWI | GGATC | 1 cut(s) | 662 |
| AcoI | YGGCCR | 3 cut(s) | 181, 340, 643 |
| AcsI | RAATTY | 1 cut(s) | 1069 |
| AcuI | CTGAAG | 1 cut(s) | 792 |
| AfaI | GTAC | 5 cut(s) | 451, 682, 775, 872, 946 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 788, 1160 |
| AgsI | TTSAA | 8 cut(s) | 370, 544, 732, 835, 929, 977, 1013, 1043 |
| AjnI | CCWGG | 2 cut(s) | 262, 622 |
| AluBI | AGCT | 2 cut(s) | 812, 819 |
| AluI | AGCT | 2 cut(s) | 812, 819 |
| Alw21I | GWGCWC | 1 cut(s) | 1036 |
| Alw26I | GTCTC | 1 cut(s) | 808 |
| AlwI | GGATC | 1 cut(s) | 662 |
| AoxI | GGCC | 4 cut(s) | 181, 340, 397, 643 |
| ApeKI | GCWGC | 1 cut(s) | 711 |
| ApoI | RAATTY | 1 cut(s) | 1069 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 631 |
| AspS9I | GGNCC | 2 cut(s) | 44, 398 |
| AsuC2I | CCSGG | 2 cut(s) | 273, 1018 |
| AvaII | GGWCC | 1 cut(s) | 44 |
| BalI | TGGCCA | 1 cut(s) | 342 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 815, 847 |
| BbsI | GAAGAC | 2 cut(s) | 27, 1267 |
| Bbv12I | GWGCWC | 1 cut(s) | 1036 |
| BbvI | GCAGC | 1 cut(s) | 698 |
| BccI | CCATC | 2 cut(s) | 236, 351 |
| BceAI | ACGGC | 2 cut(s) | 196, 630 |
| BciT130I | CCWGG | 2 cut(s) | 264, 624 |
| BclI | TGATCA | 1 cut(s) | 565 |
| BcnI | CCSGG | 2 cut(s) | 273, 1018 |
| BcoDI | GTCTC | 1 cut(s) | 808 |
| BfaI | CTAG | 3 cut(s) | 671, 735, 813 |
| BfuAI | ACCTGC | 1 cut(s) | 870 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 405 |
| BisI | GCNGC | 3 cut(s) | 582, 643, 712 |
| BlsI | GCNGC | 3 cut(s) | 583, 644, 713 |
| Bme1390I | CCNGG | 4 cut(s) | 264, 273, 624, 1018 |
| Bme18I | GGWCC | 1 cut(s) | 44 |
| BmgT120I | GGNCC | 2 cut(s) | 44, 398 |
| BmiI | GGNNCC | 3 cut(s) | 46, 261, 400 |
| BmrFI | CCNGG | 4 cut(s) | 264, 273, 624, 1018 |
| BmrI | ACTGGG | 1 cut(s) | 311 |
| BmsI | GCATC | 3 cut(s) | 606, 1111, 1259 |
| BmuI | ACTGGG | 1 cut(s) | 311 |
| BpiI | GAAGAC | 2 cut(s) | 27, 1267 |
| BpuEI | CTTGAG | 2 cut(s) | 1061, 1184 |
| BpuMI | CCSGG | 2 cut(s) | 273, 1018 |
| BsaJI | CCNNGG | 1 cut(s) | 1016 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 788, 1160 |
| Bse1I | ACTGG | 3 cut(s) | 317, 325, 559 |
| Bse3DI | GCAATG | 1 cut(s) | 19 |
| BseBI | CCWGG | 2 cut(s) | 264, 624 |
| BseDI | CCNNGG | 1 cut(s) | 1016 |
| BseGI | GGATG | 2 cut(s) | 268, 906 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 788, 1160 |
| BseMI | GCAATG | 1 cut(s) | 19 |
| BseNI | ACTGG | 3 cut(s) | 317, 325, 559 |
| BseX3I | CGGCCG | 1 cut(s) | 643 |
| BseXI | GCAGC | 1 cut(s) | 698 |
| BseYI | CCCAGC | 1 cut(s) | 47 |
| BsgI | GTGCAG | 1 cut(s) | 1002 |
| Bsh1285I | CGRYCG | 1 cut(s) | 646 |
| BshFI | GGCC | 4 cut(s) | 183, 342, 399, 645 |
| BsiEI | CGRYCG | 1 cut(s) | 646 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1036 |
| BsiSI | CCGG | 2 cut(s) | 273, 1018 |
| BslFI | GGGAC | 2 cut(s) | 299, 369 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 788, 1160 |
| BsmAI | GTCTC | 1 cut(s) | 808 |
| BsmFI | GGGAC | 2 cut(s) | 299, 369 |
| BsmI | GAATGC | 1 cut(s) | 763 |
| BsnI | GGCC | 4 cut(s) | 183, 342, 399, 645 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1036 |
| Bsp1407I | TGTACA | 2 cut(s) | 773, 870 |
| Bsp143I | GATC | 8 cut(s) | 106, 124, 187, 219, 565, 654, 1192, 1203 |
| BspACI | CCGC | 2 cut(s) | 581, 642 |
| BspANI | GGCC | 4 cut(s) | 183, 342, 399, 645 |
| BspHI | TCATGA | 1 cut(s) | 1023 |
| BspLI | GGNNCC | 3 cut(s) | 46, 261, 400 |
| BspMI | ACCTGC | 1 cut(s) | 870 |
| BspPI | GGATC | 1 cut(s) | 662 |
| BspQI | GCTCTTC | 1 cut(s) | 361 |
| BsrDI | GCAATG | 1 cut(s) | 19 |
| BsrGI | TGTACA | 2 cut(s) | 773, 870 |
| BsrI | ACTGG | 3 cut(s) | 317, 325, 559 |
| BssECI | CCNNGG | 1 cut(s) | 1016 |
| BssMI | GATC | 8 cut(s) | 106, 124, 187, 219, 565, 654, 1192, 1203 |
| Bst2UI | CCWGG | 2 cut(s) | 264, 624 |
| Bst4CI | ACNGT | 1 cut(s) | 313 |
| Bst6I | CTCTTC | 2 cut(s) | 361, 811 |
| BstAUI | TGTACA | 2 cut(s) | 773, 870 |
| BstC8I | GCNNGC | 5 cut(s) | 53, 579, 586, 757, 761 |
| BstDEI | CTNAG | 2 cut(s) | 66, 411 |
| BstF5I | GGATG | 2 cut(s) | 268, 906 |
| BstKTI | GATC | 8 cut(s) | 109, 127, 190, 222, 568, 657, 1195, 1206 |
| BstMAI | GTCTC | 1 cut(s) | 808 |
| BstMBI | GATC | 8 cut(s) | 106, 124, 187, 219, 565, 654, 1192, 1203 |
| BstMCI | CGRYCG | 1 cut(s) | 646 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 294, 360, 405, 528, 594 |
| BstNI | CCWGG | 2 cut(s) | 264, 624 |
| BstNSI | RCATGY | 4 cut(s) | 581, 759, 763, 780 |
| BstSCI | CCNGG | 4 cut(s) | 262, 271, 622, 1016 |
| BstV1I | GCAGC | 1 cut(s) | 698 |
| BstV2I | GAAGAC | 2 cut(s) | 27, 1267 |
| BstZI | CGGCCG | 1 cut(s) | 643 |
| BsuRI | GGCC | 4 cut(s) | 183, 342, 399, 645 |
| BtsCI | GGATG | 2 cut(s) | 268, 906 |
| BtsIMutI | CAGTG | 2 cut(s) | 332, 566 |
| BveI | ACCTGC | 1 cut(s) | 870 |
| Cac8I | GCNNGC | 5 cut(s) | 53, 579, 586, 757, 761 |
| CciI | TCATGA | 1 cut(s) | 1023 |
| Cfr13I | GGNCC | 2 cut(s) | 44, 398 |
| CseI | GACGC | 1 cut(s) | 1094 |
| Csp6I | GTAC | 5 cut(s) | 450, 681, 774, 871, 945 |
| CviQI | GTAC | 5 cut(s) | 450, 681, 774, 871, 945 |
| DdeI | CTNAG | 2 cut(s) | 66, 411 |
| DpnI | GATC | 8 cut(s) | 108, 126, 189, 221, 567, 656, 1194, 1205 |
| DpnII | GATC | 8 cut(s) | 106, 124, 187, 219, 565, 654, 1192, 1203 |
| EaeI | YGGCCR | 3 cut(s) | 181, 340, 643 |
| EagI | CGGCCG | 1 cut(s) | 643 |
| Eam1104I | CTCTTC | 2 cut(s) | 361, 811 |
| EarI | CTCTTC | 2 cut(s) | 361, 811 |
| EclXI | CGGCCG | 1 cut(s) | 643 |
| Eco47I | GGWCC | 1 cut(s) | 44 |
| Eco52I | CGGCCG | 1 cut(s) | 643 |
| Eco57I | CTGAAG | 1 cut(s) | 792 |
| EcoO109I | RGGNCCY | 1 cut(s) | 44 |
| EcoRII | CCWGG | 2 cut(s) | 262, 622 |
| EcoT22I | ATGCAT | 3 cut(s) | 599, 761, 765 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 670, 702, 1123, 1155 |
| FaqI | GGGAC | 2 cut(s) | 299, 369 |
| FbaI | TGATCA | 1 cut(s) | 565 |
| Fnu4HI | GCNGC | 3 cut(s) | 582, 643, 712 |
| FokI | GGATG | 2 cut(s) | 255, 893 |
| Fsp4HI | GCNGC | 3 cut(s) | 582, 643, 712 |
| FspBI | CTAG | 3 cut(s) | 671, 735, 813 |
| GluI | GCNGC | 3 cut(s) | 582, 643, 712 |
| GsaI | CCCAGC | 1 cut(s) | 51 |
| HaeIII | GGCC | 4 cut(s) | 183, 342, 399, 645 |
| HapII | CCGG | 2 cut(s) | 273, 1018 |
| HgaI | GACGC | 1 cut(s) | 1094 |
| HinfI | GANTC | 7 cut(s) | 215, 443, 674, 864, 964, 1165, 1184 |
| HpaII | CCGG | 2 cut(s) | 273, 1018 |
| Hpy166II | GTNNAC | 1 cut(s) | 871 |
| Hpy188I | TCNGA | 2 cut(s) | 61, 654 |
| Hpy188III | TCNNGA | 9 cut(s) | 80, 128, 191, 661, 671, 835, 929, 1024, 1201 |
| Hpy8I | GTNNAC | 1 cut(s) | 871 |
| HpyAV | CCTTC | 4 cut(s) | 84, 412, 935, 1058 |
| HpyCH4III | ACNGT | 1 cut(s) | 313 |
| HpyCH4IV | ACGT | 2 cut(s) | 509, 947 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 294, 360, 405, 528, 594 |
| HpyF3I | CTNAG | 2 cut(s) | 66, 411 |
| HpySE526I | ACGT | 2 cut(s) | 509, 947 |
| Ksp22I | TGATCA | 1 cut(s) | 565 |
| Kzo9I | GATC | 8 cut(s) | 106, 124, 187, 219, 565, 654, 1192, 1203 |
| LguI | GCTCTTC | 1 cut(s) | 361 |
| LmnI | GCTCC | 3 cut(s) | 259, 816, 1039 |
| Lsp1109I | GCAGC | 1 cut(s) | 698 |
| LweI | GCATC | 3 cut(s) | 606, 1111, 1259 |
| MaeI | CTAG | 3 cut(s) | 671, 735, 813 |
| MaeII | ACGT | 2 cut(s) | 509, 947 |
| MaeIII | GTNAC | 2 cut(s) | 329, 1000 |
| MalI | GATC | 8 cut(s) | 108, 126, 189, 221, 567, 656, 1194, 1205 |
| MboI | GATC | 8 cut(s) | 106, 124, 187, 219, 565, 654, 1192, 1203 |
| MfeI | CAATTG | 1 cut(s) | 1245 |
| MhlI | GDGCHC | 1 cut(s) | 1036 |
| MlsI | TGGCCA | 1 cut(s) | 342 |
| MluCI | AATT | 8 cut(s) | 291, 348, 365, 470, 525, 666, 1069, 1245 |
| MluNI | TGGCCA | 1 cut(s) | 342 |
| MmeI | TCCRAC | 3 cut(s) | 632, 1062, 1178 |
| MnlI | CCTC | 7 cut(s) | 35, 990, 1137, 1161, 1220, 1236, 1275 |
| Mox20I | TGGCCA | 1 cut(s) | 342 |
| Mph1103I | ATGCAT | 3 cut(s) | 599, 761, 765 |
| MroXI | GAANNNNTTC | 1 cut(s) | 631 |
| MscI | TGGCCA | 1 cut(s) | 342 |
| MseI | TTAA | 5 cut(s) | 254, 308, 497, 612, 1116 |
| Msp20I | TGGCCA | 1 cut(s) | 342 |
| MspI | CCGG | 2 cut(s) | 273, 1018 |
| MspR9I | CCNGG | 4 cut(s) | 264, 273, 624, 1018 |
| MunI | CAATTG | 1 cut(s) | 1245 |
| Mva1269I | GAATGC | 1 cut(s) | 763 |
| MvaI | CCWGG | 2 cut(s) | 264, 624 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 294, 360, 405, 528, 594 |
| NciI | CCSGG | 2 cut(s) | 273, 1018 |
| NdeII | GATC | 8 cut(s) | 106, 124, 187, 219, 565, 654, 1192, 1203 |
| NlaIV | GGNNCC | 3 cut(s) | 46, 261, 400 |
| NmuCI | GTSAC | 1 cut(s) | 329 |
| NsiI | ATGCAT | 3 cut(s) | 599, 761, 765 |
| NspI | RCATGY | 4 cut(s) | 581, 759, 763, 780 |
| PaeI | GCATGC | 3 cut(s) | 581, 759, 763 |
| PagI | TCATGA | 1 cut(s) | 1023 |
| PciSI | GCTCTTC | 1 cut(s) | 361 |
| PctI | GAATGC | 1 cut(s) | 763 |
| PdmI | GAANNNNTTC | 1 cut(s) | 631 |
| PfeI | GAWTC | 7 cut(s) | 215, 443, 674, 864, 964, 1165, 1184 |
| PfoI | TCCNGGA | 1 cut(s) | 271 |
| PkrI | GCNGC | 3 cut(s) | 583, 644, 713 |
| PpuMI | RGGWCCY | 1 cut(s) | 44 |
| Psp1406I | AACGTT | 1 cut(s) | 509 |
| Psp5II | RGGWCCY | 1 cut(s) | 44 |
| Psp6I | CCWGG | 2 cut(s) | 262, 622 |
| PspFI | CCCAGC | 1 cut(s) | 47 |
| PspGI | CCWGG | 2 cut(s) | 262, 622 |
| PspN4I | GGNNCC | 3 cut(s) | 46, 261, 400 |
| PspPI | GGNCC | 2 cut(s) | 44, 398 |
| PspPPI | RGGWCCY | 1 cut(s) | 44 |
| RsaI | GTAC | 5 cut(s) | 451, 682, 775, 872, 946 |
| RsaNI | GTAC | 5 cut(s) | 450, 681, 774, 871, 945 |
| SapI | GCTCTTC | 1 cut(s) | 361 |
| SaqAI | TTAA | 5 cut(s) | 254, 308, 497, 612, 1116 |
| SatI | GCNGC | 3 cut(s) | 582, 643, 712 |
| Sau3AI | GATC | 8 cut(s) | 106, 124, 187, 219, 565, 654, 1192, 1203 |
| Sau96I | GGNCC | 2 cut(s) | 44, 398 |
| ScrFI | CCNGG | 4 cut(s) | 264, 273, 624, 1018 |
| SduI | GDGCHC | 1 cut(s) | 1036 |
| SfaNI | GCATC | 3 cut(s) | 606, 1111, 1259 |
| SinI | GGWCC | 1 cut(s) | 44 |
| SmlI | CTYRAG | 2 cut(s) | 1076, 1199 |
| SmoI | CTYRAG | 2 cut(s) | 1076, 1199 |
| SphI | GCATGC | 3 cut(s) | 581, 759, 763 |
| Sse9I | AATT | 8 cut(s) | 291, 348, 365, 470, 525, 666, 1069, 1245 |
| SsiI | CCGC | 2 cut(s) | 581, 642 |
| SspMI | CTAG | 3 cut(s) | 671, 735, 813 |
| StyD4I | CCNGG | 4 cut(s) | 262, 271, 622, 1016 |
| TaaI | ACNGT | 1 cut(s) | 313 |
| TaiI | ACGT | 2 cut(s) | 512, 950 |
| TaqI | TCGA | 5 cut(s) | 218, 660, 694, 1206, 1258 |
| TasI | AATT | 8 cut(s) | 291, 348, 365, 470, 525, 666, 1069, 1245 |
| TatI | WGTACW | 4 cut(s) | 449, 680, 773, 870 |
| TauI | GCSGC | 2 cut(s) | 584, 645 |
| TfiI | GAWTC | 7 cut(s) | 215, 443, 674, 864, 964, 1165, 1184 |
| Tru1I | TTAA | 5 cut(s) | 254, 308, 497, 612, 1116 |
| Tru9I | TTAA | 5 cut(s) | 254, 308, 497, 612, 1116 |
| TscAI | CASTG | 2 cut(s) | 332, 566 |
| TseFI | GTSAC | 1 cut(s) | 329 |
| TseI | GCWGC | 1 cut(s) | 711 |
| Tsp45I | GTSAC | 1 cut(s) | 329 |
| TspDTI | ATGAA | 8 cut(s) | 17, 45, 315, 762, 954, 1012, 1040, 1176 |
| TspGWI | ACGGA | 2 cut(s) | 272, 877 |
| TspRI | CASTG | 2 cut(s) | 332, 566 |
| VpaK11BI | GGWCC | 1 cut(s) | 44 |
| XapI | RAATTY | 1 cut(s) | 1069 |
| XbaI | TCTAGA | 1 cut(s) | 670 |
| XceI | RCATGY | 4 cut(s) | 581, 759, 763, 780 |
| XmnI | GAANNNNTTC | 1 cut(s) | 631 |
| XspI | CTAG | 3 cut(s) | 671, 735, 813 |
| Zsp2I | ATGCAT | 3 cut(s) | 599, 761, 765 |