E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 46 |
| AfaI | GTAC | 1 cut(s) | 299 |
| AgsI | TTSAA | 1 cut(s) | 20 |
| AluBI | AGCT | 1 cut(s) | 203 |
| AluI | AGCT | 1 cut(s) | 203 |
| Alw21I | GWGCWC | 1 cut(s) | 13 |
| ApoI | RAATTY | 1 cut(s) | 46 |
| AsuHPI | GGTGA | 1 cut(s) | 179 |
| Bbv12I | GWGCWC | 1 cut(s) | 13 |
| BccI | CCATC | 1 cut(s) | 233 |
| BmcAI | AGTACT | 1 cut(s) | 299 |
| BmsI | GCATC | 1 cut(s) | 88 |
| BpuEI | CTTGAG | 2 cut(s) | 38, 161 |
| BsiHKAI | GWGCWC | 1 cut(s) | 13 |
| BsiSI | CCGG | 1 cut(s) | 247 |
| Bsp1286I | GDGCHC | 1 cut(s) | 13 |
| Bsp143I | GATC | 3 cut(s) | 169, 180, 277 |
| BssMI | GATC | 3 cut(s) | 169, 180, 277 |
| BstKTI | GATC | 3 cut(s) | 172, 183, 280 |
| BstMBI | GATC | 3 cut(s) | 169, 180, 277 |
| BstNSI | RCATGY | 1 cut(s) | 246 |
| CseI | GACGC | 1 cut(s) | 71 |
| Csp6I | GTAC | 1 cut(s) | 298 |
| CviAII | CATG | 2 cut(s) | 147, 243 |
| CviJI | RGCY | 3 cut(s) | 175, 203, 231 |
| CviKI_1 | RGCY | 3 cut(s) | 175, 203, 231 |
| CviQI | GTAC | 1 cut(s) | 298 |
| DpnI | GATC | 3 cut(s) | 171, 182, 279 |
| DpnII | GATC | 3 cut(s) | 169, 180, 277 |
| Eco32I | GATATC | 1 cut(s) | 264 |
| EcoRV | GATATC | 1 cut(s) | 264 |
| FaeI | CATG | 2 cut(s) | 150, 246 |
| FaiI | YATR | 7 cut(s) | 148, 194, 215, 219, 234, 236, 244 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 100, 132 |
| FatI | CATG | 2 cut(s) | 146, 242 |
| FauNDI | CATATG | 1 cut(s) | 234 |
| HapII | CCGG | 1 cut(s) | 247 |
| HgaI | GACGC | 1 cut(s) | 71 |
| Hin1II | CATG | 2 cut(s) | 150, 246 |
| HinfI | GANTC | 2 cut(s) | 142, 161 |
| HpaII | CCGG | 1 cut(s) | 247 |
| HphI | GGTGA | 1 cut(s) | 179 |
| Hpy188III | TCNNGA | 1 cut(s) | 178 |
| HpyAV | CCTTC | 1 cut(s) | 35 |
| HpyCH4V | TGCA | 1 cut(s) | 192 |
| Hsp92II | CATG | 2 cut(s) | 150, 246 |
| Kzo9I | GATC | 3 cut(s) | 169, 180, 277 |
| LmnI | GCTCC | 2 cut(s) | 16, 200 |
| LpnPI | CCDG | 2 cut(s) | 150, 260 |
| LweI | GCATC | 1 cut(s) | 88 |
| MalI | GATC | 3 cut(s) | 171, 182, 279 |
| MboI | GATC | 3 cut(s) | 169, 180, 277 |
| MhlI | GDGCHC | 1 cut(s) | 13 |
| MluCI | AATT | 4 cut(s) | 46, 210, 220, 290 |
| MmeI | TCCRAC | 1 cut(s) | 39 |
| MnlI | CCTC | 2 cut(s) | 114, 197 |
| MseI | TTAA | 2 cut(s) | 93, 293 |
| MspI | CCGG | 1 cut(s) | 247 |
| NdeI | CATATG | 1 cut(s) | 234 |
| NdeII | GATC | 3 cut(s) | 169, 180, 277 |
| NlaIII | CATG | 2 cut(s) | 150, 246 |
| NspI | RCATGY | 1 cut(s) | 246 |
| PfeI | GAWTC | 2 cut(s) | 142, 161 |
| RsaI | GTAC | 1 cut(s) | 299 |
| RsaNI | GTAC | 1 cut(s) | 298 |
| SaqAI | TTAA | 2 cut(s) | 93, 293 |
| Sau3AI | GATC | 3 cut(s) | 169, 180, 277 |
| ScaI | AGTACT | 1 cut(s) | 299 |
| SduI | GDGCHC | 1 cut(s) | 13 |
| SetI | ASST | 4 cut(s) | 10, 169, 189, 205 |
| SfaNI | GCATC | 1 cut(s) | 88 |
| SmlI | CTYRAG | 2 cut(s) | 53, 176 |
| SmoI | CTYRAG | 2 cut(s) | 53, 176 |
| Sse9I | AATT | 4 cut(s) | 46, 210, 220, 290 |
| TaqI | TCGA | 1 cut(s) | 183 |
| TasI | AATT | 4 cut(s) | 46, 210, 220, 290 |
| TatI | WGTACW | 1 cut(s) | 297 |
| TfiI | GAWTC | 2 cut(s) | 142, 161 |
| Tru1I | TTAA | 2 cut(s) | 93, 293 |
| Tru9I | TTAA | 2 cut(s) | 93, 293 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| XapI | RAATTY | 1 cut(s) | 46 |
| XceI | RCATGY | 1 cut(s) | 246 |
| ZrmI | AGTACT | 1 cut(s) | 299 |