E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 186, 334 |
| AccII | CGCG | 1 cut(s) | 27 |
| AccIII | TCCGGA | 1 cut(s) | 152 |
| AciI | CCGC | 2 cut(s) | 126, 375 |
| AclWI | GGATC | 2 cut(s) | 435, 608 |
| AcoI | YGGCCR | 1 cut(s) | 544 |
| AcsI | RAATTY | 1 cut(s) | 583 |
| AfaI | GTAC | 6 cut(s) | 60, 176, 204, 396, 504, 639 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 137, 391, 491 |
| AflIII | ACRYGT | 1 cut(s) | 25 |
| AgsI | TTSAA | 1 cut(s) | 686 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 392, 424 |
| AluBI | AGCT | 6 cut(s) | 35, 89, 110, 167, 188, 217 |
| AluI | AGCT | 6 cut(s) | 35, 89, 110, 167, 188, 217 |
| Alw26I | GTCTC | 2 cut(s) | 146, 564 |
| AlwI | GGATC | 2 cut(s) | 435, 608 |
| Aor13HI | TCCGGA | 1 cut(s) | 152 |
| AoxI | GGCC | 1 cut(s) | 544 |
| ApoI | RAATTY | 1 cut(s) | 583 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 404 |
| AspS9I | GGNCC | 1 cut(s) | 128 |
| AsuHPI | GGTGA | 2 cut(s) | 282, 478 |
| AvaII | GGWCC | 1 cut(s) | 128 |
| BalI | TGGCCA | 1 cut(s) | 546 |
| BbsI | GAAGAC | 2 cut(s) | 188, 530 |
| BciVI | GTATCC | 1 cut(s) | 160 |
| BclI | TGATCA | 1 cut(s) | 268 |
| BcoDI | GTCTC | 2 cut(s) | 146, 564 |
| BfaI | CTAG | 3 cut(s) | 81, 200, 206 |
| BfmI | CTRYAG | 2 cut(s) | 90, 331 |
| BfuAI | ACCTGC | 2 cut(s) | 186, 334 |
| BfuI | GTATCC | 1 cut(s) | 160 |
| BisI | GCNGC | 1 cut(s) | 375 |
| BlsI | GCNGC | 1 cut(s) | 376 |
| BmcAI | AGTACT | 1 cut(s) | 204 |
| Bme18I | GGWCC | 1 cut(s) | 128 |
| BmgT120I | GGNCC | 1 cut(s) | 128 |
| BpiI | GAAGAC | 2 cut(s) | 188, 530 |
| BpuEI | CTTGAG | 1 cut(s) | 469 |
| BsaI | GGTCTC | 2 cut(s) | 146, 564 |
| BsaJI | CCNNGG | 1 cut(s) | 617 |
| BsaWI | WCCGGW | 1 cut(s) | 152 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 137, 391, 491 |
| BseAI | TCCGGA | 1 cut(s) | 152 |
| BseDI | CCNNGG | 1 cut(s) | 617 |
| BseGI | GGATG | 1 cut(s) | 282 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 137, 391, 491 |
| BseMII | CTCAG | 2 cut(s) | 102, 351 |
| BseYI | CCCAGC | 2 cut(s) | 35, 525 |
| Bsh1236I | CGCG | 1 cut(s) | 27 |
| BshFI | GGCC | 1 cut(s) | 546 |
| BsiSI | CCGG | 1 cut(s) | 153 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 137, 391, 491 |
| BsmAI | GTCTC | 2 cut(s) | 146, 564 |
| BsmI | GAATGC | 2 cut(s) | 13, 16 |
| BsnI | GGCC | 1 cut(s) | 546 |
| Bso31I | GGTCTC | 2 cut(s) | 146, 564 |
| Bsp13I | TCCGGA | 1 cut(s) | 152 |
| Bsp1407I | TGTACA | 1 cut(s) | 58 |
| Bsp143I | GATC | 4 cut(s) | 268, 415, 427, 613 |
| BspACI | CCGC | 2 cut(s) | 126, 375 |
| BspANI | GGCC | 1 cut(s) | 546 |
| BspCNI | CTCAG | 2 cut(s) | 103, 352 |
| BspEI | TCCGGA | 1 cut(s) | 152 |
| BspFNI | CGCG | 1 cut(s) | 27 |
| BspMI | ACCTGC | 2 cut(s) | 186, 334 |
| BspPI | GGATC | 2 cut(s) | 435, 608 |
| BspTNI | GGTCTC | 2 cut(s) | 146, 564 |
| BsrGI | TGTACA | 1 cut(s) | 58 |
| BssECI | CCNNGG | 1 cut(s) | 617 |
| BssMI | GATC | 4 cut(s) | 268, 415, 427, 613 |
| Bst4CI | ACNGT | 2 cut(s) | 405, 461 |
| Bst6I | CTCTTC | 1 cut(s) | 681 |
| BstAUI | TGTACA | 1 cut(s) | 58 |
| BstC8I | GCNNGC | 1 cut(s) | 680 |
| BstDEI | CTNAG | 3 cut(s) | 111, 306, 360 |
| BstEII | GGTNACC | 1 cut(s) | 73 |
| BstF5I | GGATG | 1 cut(s) | 282 |
| BstFNI | CGCG | 1 cut(s) | 27 |
| BstKTI | GATC | 4 cut(s) | 271, 418, 430, 616 |
| BstMAI | GTCTC | 2 cut(s) | 146, 564 |
| BstMBI | GATC | 4 cut(s) | 268, 415, 427, 613 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 223 |
| BstPI | GGTNACC | 1 cut(s) | 73 |
| BstSFI | CTRYAG | 2 cut(s) | 90, 331 |
| BstUI | CGCG | 1 cut(s) | 27 |
| BstV2I | GAAGAC | 2 cut(s) | 188, 530 |
| BsuI | GTATCC | 1 cut(s) | 160 |
| BsuRI | GGCC | 1 cut(s) | 546 |
| BtsCI | GGATG | 1 cut(s) | 282 |
| BveI | ACCTGC | 2 cut(s) | 186, 334 |
| Cac8I | GCNNGC | 1 cut(s) | 680 |
| Cfr13I | GGNCC | 1 cut(s) | 128 |
| CpoI | CGGWCCG | 1 cut(s) | 128 |
| CseI | GACGC | 1 cut(s) | 16 |
| Csp6I | GTAC | 6 cut(s) | 59, 175, 203, 395, 503, 638 |
| CspI | CGGWCCG | 1 cut(s) | 128 |
| CviAII | CATG | 2 cut(s) | 266, 530 |
| CviQI | GTAC | 6 cut(s) | 59, 175, 203, 395, 503, 638 |
| DdeI | CTNAG | 3 cut(s) | 111, 306, 360 |
| DpnI | GATC | 4 cut(s) | 270, 417, 429, 615 |
| DpnII | GATC | 4 cut(s) | 268, 415, 427, 613 |
| EaeI | YGGCCR | 1 cut(s) | 544 |
| Eam1104I | CTCTTC | 1 cut(s) | 681 |
| EarI | CTCTTC | 1 cut(s) | 681 |
| Eco31I | GGTCTC | 2 cut(s) | 146, 564 |
| Eco47I | GGWCC | 1 cut(s) | 128 |
| Eco91I | GGTNACC | 1 cut(s) | 73 |
| EcoO65I | GGTNACC | 1 cut(s) | 73 |
| FaeI | CATG | 2 cut(s) | 269, 533 |
| FaiI | YATR | 8 cut(s) | 63, 185, 246, 267, 333, 531, 657, 659 |
| FatI | CATG | 2 cut(s) | 265, 529 |
| FbaI | TGATCA | 1 cut(s) | 268 |
| Fnu4HI | GCNGC | 1 cut(s) | 375 |
| FokI | GGATG | 1 cut(s) | 289 |
| Fsp4HI | GCNGC | 1 cut(s) | 375 |
| FspBI | CTAG | 3 cut(s) | 81, 200, 206 |
| GluI | GCNGC | 1 cut(s) | 375 |
| GsaI | CCCAGC | 2 cut(s) | 39, 529 |
| HaeIII | GGCC | 1 cut(s) | 546 |
| HapII | CCGG | 1 cut(s) | 153 |
| HgaI | GACGC | 1 cut(s) | 16 |
| Hin1II | CATG | 2 cut(s) | 269, 533 |
| HindIII | AAGCTT | 1 cut(s) | 165 |
| HinfI | GANTC | 3 cut(s) | 133, 287, 323 |
| HpaII | CCGG | 1 cut(s) | 153 |
| HphI | GGTGA | 2 cut(s) | 282, 478 |
| Hpy166II | GTNNAC | 1 cut(s) | 59 |
| Hpy188I | TCNGA | 4 cut(s) | 32, 132, 569, 579 |
| Hpy188III | TCNNGA | 3 cut(s) | 81, 153, 425 |
| Hpy8I | GTNNAC | 1 cut(s) | 59 |
| Hpy99I | CGWCG | 1 cut(s) | 494 |
| HpyAV | CCTTC | 1 cut(s) | 584 |
| HpyCH4III | ACNGT | 2 cut(s) | 405, 461 |
| HpyCH4V | TGCA | 5 cut(s) | 11, 226, 301, 343, 678 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 223 |
| HpyF3I | CTNAG | 3 cut(s) | 111, 306, 360 |
| Hsp92II | CATG | 2 cut(s) | 269, 533 |
| Kpn2I | TCCGGA | 1 cut(s) | 152 |
| Ksp22I | TGATCA | 1 cut(s) | 268 |
| Kzo9I | GATC | 4 cut(s) | 268, 415, 427, 613 |
| MaeI | CTAG | 3 cut(s) | 81, 200, 206 |
| MaeIII | GTNAC | 2 cut(s) | 73, 284 |
| MalI | GATC | 4 cut(s) | 270, 417, 429, 615 |
| MboI | GATC | 4 cut(s) | 268, 415, 427, 613 |
| MboII | GAAGA | 3 cut(s) | 12, 188, 530 |
| MlsI | TGGCCA | 1 cut(s) | 546 |
| MluCI | AATT | 2 cut(s) | 510, 583 |
| MluI | ACGCGT | 1 cut(s) | 25 |
| MluNI | TGGCCA | 1 cut(s) | 546 |
| MlyI | GAGTC | 2 cut(s) | 142, 281 |
| MnlI | CCTC | 8 cut(s) | 88, 88, 226, 495, 627, 657, 665, 682 |
| Mox20I | TGGCCA | 1 cut(s) | 546 |
| MroI | TCCGGA | 1 cut(s) | 152 |
| MroXI | GAANNNNTTC | 1 cut(s) | 404 |
| MscI | TGGCCA | 1 cut(s) | 546 |
| MseI | TTAA | 4 cut(s) | 252, 348, 356, 498 |
| Msp20I | TGGCCA | 1 cut(s) | 546 |
| MspI | CCGG | 1 cut(s) | 153 |
| Mva1269I | GAATGC | 2 cut(s) | 13, 16 |
| MvnI | CGCG | 1 cut(s) | 27 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 223 |
| NdeII | GATC | 4 cut(s) | 268, 415, 427, 613 |
| NlaIII | CATG | 2 cut(s) | 269, 533 |
| NmeAIII | GCCGAG | 1 cut(s) | 598 |
| NmuCI | GTSAC | 1 cut(s) | 284 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 128, 625 |
| PctI | GAATGC | 2 cut(s) | 13, 16 |
| PdmI | GAANNNNTTC | 1 cut(s) | 404 |
| PfeI | GAWTC | 1 cut(s) | 323 |
| PkrI | GCNGC | 1 cut(s) | 376 |
| PleI | GAGTC | 2 cut(s) | 141, 281 |
| PpsI | GAGTC | 2 cut(s) | 141, 281 |
| PspEI | GGTNACC | 1 cut(s) | 73 |
| PspFI | CCCAGC | 2 cut(s) | 35, 525 |
| PspPI | GGNCC | 1 cut(s) | 128 |
| RsaI | GTAC | 6 cut(s) | 60, 176, 204, 396, 504, 639 |
| RsaNI | GTAC | 6 cut(s) | 59, 175, 203, 395, 503, 638 |
| Rsr2I | CGGWCCG | 1 cut(s) | 128 |
| RsrII | CGGWCCG | 1 cut(s) | 128 |
| SaqAI | TTAA | 4 cut(s) | 252, 348, 356, 498 |
| SatI | GCNGC | 1 cut(s) | 375 |
| Sau3AI | GATC | 4 cut(s) | 268, 415, 427, 613 |
| Sau96I | GGNCC | 1 cut(s) | 128 |
| ScaI | AGTACT | 1 cut(s) | 204 |
| SchI | GAGTC | 2 cut(s) | 142, 281 |
| SfcI | CTRYAG | 2 cut(s) | 90, 331 |
| SinI | GGWCC | 1 cut(s) | 128 |
| SmlI | CTYRAG | 1 cut(s) | 448 |
| SmoI | CTYRAG | 1 cut(s) | 448 |
| Sse9I | AATT | 2 cut(s) | 510, 583 |
| SsiI | CCGC | 2 cut(s) | 126, 375 |
| SspMI | CTAG | 3 cut(s) | 81, 200, 206 |
| TaaI | ACNGT | 2 cut(s) | 405, 461 |
| TasI | AATT | 2 cut(s) | 510, 583 |
| TatI | WGTACW | 3 cut(s) | 58, 202, 502 |
| TauI | GCSGC | 1 cut(s) | 377 |
| TfiI | GAWTC | 1 cut(s) | 323 |
| Tru1I | TTAA | 4 cut(s) | 252, 348, 356, 498 |
| Tru9I | TTAA | 4 cut(s) | 252, 348, 356, 498 |
| TseFI | GTSAC | 1 cut(s) | 284 |
| Tsp45I | GTSAC | 1 cut(s) | 284 |
| TspDTI | ATGAA | 2 cut(s) | 20, 596 |
| TspGWI | ACGGA | 1 cut(s) | 625 |
| VpaK11BI | GGWCC | 1 cut(s) | 128 |
| XapI | RAATTY | 1 cut(s) | 583 |
| XbaI | TCTAGA | 1 cut(s) | 80 |
| XmnI | GAANNNNTTC | 1 cut(s) | 404 |
| XspI | CTAG | 3 cut(s) | 81, 200, 206 |
| ZrmI | AGTACT | 1 cut(s) | 204 |