E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 498, 995 |
| AatII | GACGTC | 1 cut(s) | 70 |
| AciI | CCGC | 1 cut(s) | 21 |
| AcoI | YGGCCR | 1 cut(s) | 1024 |
| AcsI | RAATTY | 3 cut(s) | 242, 770, 831 |
| AcuI | CTGAAG | 1 cut(s) | 252 |
| AcyI | GRCGYC | 1 cut(s) | 67 |
| AfaI | GTAC | 2 cut(s) | 731, 952 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 330, 647 |
| AgsI | TTSAA | 5 cut(s) | 669, 709, 747, 785, 866 |
| AjiI | CACGTC | 1 cut(s) | 585 |
| AjnI | CCWGG | 3 cut(s) | 53, 482, 1000 |
| AluBI | AGCT | 5 cut(s) | 89, 299, 434, 741, 1030 |
| AluI | AGCT | 5 cut(s) | 89, 299, 434, 741, 1030 |
| Alw26I | GTCTC | 3 cut(s) | 58, 299, 667 |
| Ama87I | CYCGRG | 1 cut(s) | 254 |
| AoxI | GGCC | 2 cut(s) | 485, 1024 |
| ApeKI | GCWGC | 6 cut(s) | 48, 89, 296, 447, 651, 738 |
| ApoI | RAATTY | 3 cut(s) | 242, 770, 831 |
| AspS9I | GGNCC | 3 cut(s) | 41, 267, 486 |
| AsuHPI | GGTGA | 5 cut(s) | 248, 337, 618, 859, 1016 |
| AvaI | CYCGRG | 1 cut(s) | 254 |
| AvaII | GGWCC | 2 cut(s) | 41, 267 |
| BalI | TGGCCA | 1 cut(s) | 1026 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 665, 697 |
| BbsI | GAAGAC | 1 cut(s) | 400 |
| BbvI | GCAGC | 6 cut(s) | 60, 76, 308, 459, 638, 750 |
| BccI | CCATC | 5 cut(s) | 26, 350, 791, 947, 1030 |
| BciT130I | CCWGG | 3 cut(s) | 55, 484, 1002 |
| BcoDI | GTCTC | 3 cut(s) | 58, 299, 667 |
| BfaI | CTAG | 3 cut(s) | 527, 836, 870 |
| BfmI | CTRYAG | 2 cut(s) | 128, 445 |
| BisI | GCNGC | 6 cut(s) | 49, 90, 297, 448, 652, 739 |
| BlsI | GCNGC | 6 cut(s) | 50, 91, 298, 449, 653, 740 |
| BmcAI | AGTACT | 1 cut(s) | 952 |
| Bme1390I | CCNGG | 3 cut(s) | 55, 484, 1002 |
| Bme18I | GGWCC | 2 cut(s) | 41, 267 |
| BmeT110I | CYCGRG | 1 cut(s) | 254 |
| BmgBI | CACGTC | 1 cut(s) | 585 |
| BmgT120I | GGNCC | 3 cut(s) | 41, 267, 486 |
| BmrFI | CCNGG | 3 cut(s) | 55, 484, 1002 |
| BmsI | GCATC | 1 cut(s) | 282 |
| BpiI | GAAGAC | 1 cut(s) | 400 |
| BpuEI | CTTGAG | 2 cut(s) | 285, 1016 |
| BsaBI | GATNNNNATC | 2 cut(s) | 341, 1040 |
| BsaHI | GRCGYC | 1 cut(s) | 67 |
| BsaJI | CCNNGG | 6 cut(s) | 53, 263, 291, 324, 912, 1000 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 330, 647 |
| Bse1I | ACTGG | 1 cut(s) | 412 |
| Bse8I | GATNNNNATC | 2 cut(s) | 341, 1040 |
| BseBI | CCWGG | 3 cut(s) | 55, 484, 1002 |
| BseDI | CCNNGG | 6 cut(s) | 53, 263, 291, 324, 912, 1000 |
| BseGI | GGATG | 2 cut(s) | 585, 923 |
| BseJI | GATNNNNATC | 2 cut(s) | 341, 1040 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 330, 647 |
| BseMII | CTCAG | 1 cut(s) | 940 |
| BseNI | ACTGG | 1 cut(s) | 412 |
| BseRI | GAGGAG | 4 cut(s) | 129, 132, 135, 430 |
| BseXI | GCAGC | 6 cut(s) | 60, 76, 308, 459, 638, 750 |
| BshFI | GGCC | 2 cut(s) | 487, 1026 |
| BsiHKCI | CYCGRG | 1 cut(s) | 254 |
| BslFI | GGGAC | 2 cut(s) | 360, 386 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 330, 647 |
| BsmAI | GTCTC | 3 cut(s) | 58, 299, 667 |
| BsmBI | CGTCTC | 1 cut(s) | 58 |
| BsmFI | GGGAC | 2 cut(s) | 360, 386 |
| BsmI | GAATGC | 1 cut(s) | 622 |
| BsnI | GGCC | 2 cut(s) | 487, 1026 |
| BsoBI | CYCGRG | 1 cut(s) | 254 |
| Bsp1407I | TGTACA | 1 cut(s) | 729 |
| Bsp143I | GATC | 1 cut(s) | 895 |
| BspACI | CCGC | 1 cut(s) | 21 |
| BspANI | GGCC | 2 cut(s) | 487, 1026 |
| BspCNI | CTCAG | 1 cut(s) | 939 |
| BspMAI | CTGCAG | 1 cut(s) | 449 |
| BsrGI | TGTACA | 1 cut(s) | 729 |
| BsrI | ACTGG | 1 cut(s) | 412 |
| BssECI | CCNNGG | 6 cut(s) | 53, 263, 291, 324, 912, 1000 |
| BssMI | GATC | 1 cut(s) | 895 |
| BssNI | GRCGYC | 1 cut(s) | 67 |
| BssT1I | CCWWGG | 1 cut(s) | 912 |
| Bst2UI | CCWGG | 3 cut(s) | 55, 484, 1002 |
| Bst4CI | ACNGT | 4 cut(s) | 97, 400, 936, 950 |
| Bst6I | CTCTTC | 4 cut(s) | 56, 156, 192, 670 |
| BstACI | GRCGYC | 1 cut(s) | 67 |
| BstAUI | TGTACA | 1 cut(s) | 729 |
| BstC8I | GCNNGC | 2 cut(s) | 436, 1028 |
| BstDEI | CTNAG | 2 cut(s) | 854, 926 |
| BstDSI | CCRYGG | 1 cut(s) | 324 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 645 |
| BstF5I | GGATG | 2 cut(s) | 585, 923 |
| BstKTI | GATC | 1 cut(s) | 898 |
| BstMAI | GTCTC | 3 cut(s) | 58, 299, 667 |
| BstMBI | GATC | 1 cut(s) | 895 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 98, 296, 444 |
| BstNI | CCWGG | 3 cut(s) | 55, 484, 1002 |
| BstNSI | RCATGY | 1 cut(s) | 618 |
| BstSCI | CCNGG | 3 cut(s) | 53, 482, 1000 |
| BstSFI | CTRYAG | 2 cut(s) | 128, 445 |
| BstV1I | GCAGC | 6 cut(s) | 60, 76, 308, 459, 638, 750 |
| BstV2I | GAAGAC | 1 cut(s) | 400 |
| BsuRI | GGCC | 2 cut(s) | 487, 1026 |
| BtgI | CCRYGG | 1 cut(s) | 324 |
| BtrI | CACGTC | 1 cut(s) | 585 |
| BtsCI | GGATG | 2 cut(s) | 585, 923 |
| BtsIMutI | CAGTG | 1 cut(s) | 419 |
| Cac8I | GCNNGC | 2 cut(s) | 436, 1028 |
| Cfr13I | GGNCC | 3 cut(s) | 41, 267, 486 |
| Csp6I | GTAC | 2 cut(s) | 730, 951 |
| CviAII | CATG | 5 cut(s) | 477, 546, 605, 615, 636 |
| CviQI | GTAC | 2 cut(s) | 730, 951 |
| DdeI | CTNAG | 2 cut(s) | 854, 926 |
| DpnI | GATC | 1 cut(s) | 897 |
| DpnII | GATC | 1 cut(s) | 895 |
| EaeI | YGGCCR | 1 cut(s) | 1024 |
| Eam1104I | CTCTTC | 4 cut(s) | 56, 156, 192, 670 |
| EarI | CTCTTC | 4 cut(s) | 56, 156, 192, 670 |
| EciI | GGCGGA | 1 cut(s) | 36 |
| Eco130I | CCWWGG | 1 cut(s) | 912 |
| Eco32I | GATATC | 1 cut(s) | 1042 |
| Eco47I | GGWCC | 2 cut(s) | 41, 267 |
| Eco57I | CTGAAG | 1 cut(s) | 252 |
| Eco88I | CYCGRG | 1 cut(s) | 254 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 645 |
| EcoRI | GAATTC | 1 cut(s) | 242 |
| EcoRII | CCWGG | 3 cut(s) | 53, 482, 1000 |
| EcoRV | GATATC | 1 cut(s) | 1042 |
| EcoT14I | CCWWGG | 1 cut(s) | 912 |
| EcoT22I | ATGCAT | 1 cut(s) | 624 |
| ErhI | CCWWGG | 1 cut(s) | 912 |
| Esp3I | CGTCTC | 1 cut(s) | 58 |
| FaeI | CATG | 5 cut(s) | 480, 549, 608, 618, 639 |
| FaqI | GGGAC | 2 cut(s) | 360, 386 |
| FatI | CATG | 5 cut(s) | 476, 545, 604, 614, 635 |
| FauNDI | CATATG | 1 cut(s) | 1049 |
| Fnu4HI | GCNGC | 6 cut(s) | 49, 90, 297, 448, 652, 739 |
| FokI | GGATG | 2 cut(s) | 592, 930 |
| Fsp4HI | GCNGC | 6 cut(s) | 49, 90, 297, 448, 652, 739 |
| FspBI | CTAG | 3 cut(s) | 527, 836, 870 |
| GluI | GCNGC | 6 cut(s) | 49, 90, 297, 448, 652, 739 |
| HaeIII | GGCC | 2 cut(s) | 487, 1026 |
| Hin1I | GRCGYC | 1 cut(s) | 67 |
| Hin1II | CATG | 5 cut(s) | 480, 549, 608, 618, 639 |
| HinfI | GANTC | 6 cut(s) | 9, 146, 209, 257, 389, 530 |
| HphI | GGTGA | 5 cut(s) | 248, 337, 618, 859, 1016 |
| Hpy166II | GTNNAC | 1 cut(s) | 923 |
| Hpy188I | TCNGA | 4 cut(s) | 84, 157, 214, 929 |
| Hpy188III | TCNNGA | 4 cut(s) | 302, 527, 785, 1033 |
| Hpy8I | GTNNAC | 1 cut(s) | 923 |
| Hpy99I | CGWCG | 1 cut(s) | 72 |
| HpyAV | CCTTC | 4 cut(s) | 32, 227, 483, 1087 |
| HpyCH4III | ACNGT | 4 cut(s) | 97, 400, 936, 950 |
| HpyCH4IV | ACGT | 2 cut(s) | 67, 584 |
| HpyCH4V | TGCA | 7 cut(s) | 447, 570, 588, 622, 654, 716, 1047 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 98, 296, 444 |
| HpyF3I | CTNAG | 2 cut(s) | 854, 926 |
| HpySE526I | ACGT | 2 cut(s) | 67, 584 |
| Hsp92I | GRCGYC | 1 cut(s) | 67 |
| Hsp92II | CATG | 5 cut(s) | 480, 549, 608, 618, 639 |
| Kzo9I | GATC | 1 cut(s) | 895 |
| LmnI | GCTCC | 2 cut(s) | 443, 992 |
| Lsp1109I | GCAGC | 6 cut(s) | 60, 76, 308, 459, 638, 750 |
| LweI | GCATC | 1 cut(s) | 282 |
| MaeI | CTAG | 3 cut(s) | 527, 836, 870 |
| MaeII | ACGT | 2 cut(s) | 67, 584 |
| MaeIII | GTNAC | 2 cut(s) | 247, 1093 |
| MalI | GATC | 1 cut(s) | 897 |
| MboI | GATC | 1 cut(s) | 895 |
| MlsI | TGGCCA | 1 cut(s) | 1026 |
| MluCI | AATT | 6 cut(s) | 242, 278, 534, 747, 770, 831 |
| MluNI | TGGCCA | 1 cut(s) | 1026 |
| MlyI | GAGTC | 1 cut(s) | 266 |
| Mox20I | TGGCCA | 1 cut(s) | 1026 |
| Mph1103I | ATGCAT | 1 cut(s) | 624 |
| MscI | TGGCCA | 1 cut(s) | 1026 |
| MseI | TTAA | 3 cut(s) | 459, 762, 1099 |
| Msp20I | TGGCCA | 1 cut(s) | 1026 |
| MspA1I | CMGCKG | 1 cut(s) | 89 |
| MspR9I | CCNGG | 3 cut(s) | 55, 484, 1002 |
| Mva1269I | GAATGC | 1 cut(s) | 622 |
| MvaI | CCWGG | 3 cut(s) | 55, 484, 1002 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 98, 296, 444 |
| NdeI | CATATG | 1 cut(s) | 1049 |
| NdeII | GATC | 1 cut(s) | 895 |
| NlaIII | CATG | 5 cut(s) | 480, 549, 608, 618, 639 |
| NmeAIII | GCCGAG | 2 cut(s) | 272, 288 |
| NsiI | ATGCAT | 1 cut(s) | 624 |
| NspI | RCATGY | 1 cut(s) | 618 |
| PaeR7I | CTCGAG | 1 cut(s) | 254 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 11, 252, 332 |
| PctI | GAATGC | 1 cut(s) | 622 |
| PfeI | GAWTC | 5 cut(s) | 9, 146, 209, 389, 530 |
| PflFI | GACNNNGTC | 1 cut(s) | 69 |
| PkrI | GCNGC | 6 cut(s) | 50, 91, 298, 449, 653, 740 |
| PleI | GAGTC | 1 cut(s) | 265 |
| PpsI | GAGTC | 1 cut(s) | 265 |
| PsiI | TTATAA | 2 cut(s) | 498, 995 |
| Psp6I | CCWGG | 3 cut(s) | 53, 482, 1000 |
| PspGI | CCWGG | 3 cut(s) | 53, 482, 1000 |
| PspPI | GGNCC | 3 cut(s) | 41, 267, 486 |
| PspXI | VCTCGAGB | 1 cut(s) | 254 |
| PstI | CTGCAG | 1 cut(s) | 449 |
| PsyI | GACNNNGTC | 1 cut(s) | 69 |
| PvuII | CAGCTG | 1 cut(s) | 89 |
| RsaI | GTAC | 2 cut(s) | 731, 952 |
| RsaNI | GTAC | 2 cut(s) | 730, 951 |
| SaqAI | TTAA | 3 cut(s) | 459, 762, 1099 |
| SatI | GCNGC | 6 cut(s) | 49, 90, 297, 448, 652, 739 |
| Sau3AI | GATC | 1 cut(s) | 895 |
| Sau96I | GGNCC | 3 cut(s) | 41, 267, 486 |
| ScaI | AGTACT | 1 cut(s) | 952 |
| SchI | GAGTC | 1 cut(s) | 266 |
| ScrFI | CCNGG | 3 cut(s) | 55, 484, 1002 |
| SfaNI | GCATC | 1 cut(s) | 282 |
| SfcI | CTRYAG | 2 cut(s) | 128, 445 |
| Sfr274I | CTCGAG | 1 cut(s) | 254 |
| SinI | GGWCC | 2 cut(s) | 41, 267 |
| SlaI | CTCGAG | 1 cut(s) | 254 |
| SmlI | CTYRAG | 3 cut(s) | 254, 300, 1031 |
| SmoI | CTYRAG | 3 cut(s) | 254, 300, 1031 |
| Sse9I | AATT | 6 cut(s) | 242, 278, 534, 747, 770, 831 |
| SsiI | CCGC | 1 cut(s) | 21 |
| SspMI | CTAG | 3 cut(s) | 527, 836, 870 |
| StyD4I | CCNGG | 3 cut(s) | 53, 482, 1000 |
| StyI | CCWWGG | 1 cut(s) | 912 |
| TaaI | ACNGT | 4 cut(s) | 97, 400, 936, 950 |
| TaiI | ACGT | 2 cut(s) | 70, 587 |
| TaqI | TCGA | 3 cut(s) | 255, 276, 335 |
| TaqII | GACCGA | 2 cut(s) | 58, 368 |
| TasI | AATT | 6 cut(s) | 242, 278, 534, 747, 770, 831 |
| TatI | WGTACW | 2 cut(s) | 729, 950 |
| TfiI | GAWTC | 5 cut(s) | 9, 146, 209, 389, 530 |
| Tru1I | TTAA | 3 cut(s) | 459, 762, 1099 |
| Tru9I | TTAA | 3 cut(s) | 459, 762, 1099 |
| TscAI | CASTG | 1 cut(s) | 419 |
| TseI | GCWGC | 6 cut(s) | 48, 89, 296, 447, 651, 738 |
| TspDTI | ATGAA | 7 cut(s) | 42, 255, 465, 615, 621, 624, 969 |
| TspGWI | ACGGA | 3 cut(s) | 341, 592, 1062 |
| TspRI | CASTG | 1 cut(s) | 419 |
| Tth111I | GACNNNGTC | 1 cut(s) | 69 |
| VpaK11BI | GGWCC | 2 cut(s) | 41, 267 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 645 |
| XapI | RAATTY | 3 cut(s) | 242, 770, 831 |
| XbaI | TCTAGA | 1 cut(s) | 526 |
| XceI | RCATGY | 1 cut(s) | 618 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 277, 419 |
| XhoI | CTCGAG | 1 cut(s) | 254 |
| XspI | CTAG | 3 cut(s) | 527, 836, 870 |
| ZraI | GACGTC | 1 cut(s) | 68 |
| ZrmI | AGTACT | 1 cut(s) | 952 |
| Zsp2I | ATGCAT | 1 cut(s) | 624 |