E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 902 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 875 |
| AccII | CGCG | 1 cut(s) | 650 |
| AccIII | TCCGGA | 1 cut(s) | 976 |
| AciI | CCGC | 3 cut(s) | 373, 650, 1054 |
| AclWI | GGATC | 1 cut(s) | 672 |
| AcuI | CTGAAG | 2 cut(s) | 755, 788 |
| AfaI | GTAC | 5 cut(s) | 129, 244, 659, 768, 1039 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 827, 875, 976 |
| AflIII | ACRYGT | 1 cut(s) | 707 |
| AgsI | TTSAA | 3 cut(s) | 92, 323, 814 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 751 |
| AjnI | CCWGG | 1 cut(s) | 868 |
| AluBI | AGCT | 7 cut(s) | 47, 151, 227, 466, 609, 895, 966 |
| AluI | AGCT | 7 cut(s) | 47, 151, 227, 466, 609, 895, 966 |
| Alw26I | GTCTC | 1 cut(s) | 557 |
| AlwI | GGATC | 1 cut(s) | 672 |
| Aor13HI | TCCGGA | 1 cut(s) | 976 |
| ApeKI | GCWGC | 5 cut(s) | 54, 555, 606, 683, 1056 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 986 |
| AspS9I | GGNCC | 1 cut(s) | 788 |
| AsuHPI | GGTGA | 2 cut(s) | 16, 181 |
| AvaII | GGWCC | 1 cut(s) | 788 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 844, 876 |
| BbsI | GAAGAC | 1 cut(s) | 1092 |
| BbvI | GCAGC | 5 cut(s) | 66, 542, 618, 670, 1068 |
| BccI | CCATC | 3 cut(s) | 454, 779, 1015 |
| BceAI | ACGGC | 3 cut(s) | 42, 430, 924 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 730, 764 |
| BciT130I | CCWGG | 1 cut(s) | 870 |
| BciVI | GTATCC | 1 cut(s) | 1061 |
| BcoDI | GTCTC | 1 cut(s) | 557 |
| BfaI | CTAG | 4 cut(s) | 152, 300, 467, 1061 |
| BfmI | CTRYAG | 1 cut(s) | 670 |
| BfuAI | ACCTGC | 1 cut(s) | 902 |
| BfuI | GTATCC | 1 cut(s) | 1061 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 552 |
| BglII | AGATCT | 2 cut(s) | 154, 469 |
| BisI | GCNGC | 6 cut(s) | 55, 373, 556, 607, 684, 1057 |
| BlsI | GCNGC | 6 cut(s) | 56, 374, 557, 608, 685, 1058 |
| Bme1390I | CCNGG | 1 cut(s) | 870 |
| Bme18I | GGWCC | 1 cut(s) | 788 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 751 |
| BmgT120I | GGNCC | 1 cut(s) | 788 |
| BmiI | GGNNCC | 1 cut(s) | 819 |
| BmrFI | CCNGG | 1 cut(s) | 870 |
| BpiI | GAAGAC | 1 cut(s) | 1092 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 554, 586 |
| BpmI | CTGGAG | 2 cut(s) | 375, 852 |
| BpuEI | CTTGAG | 1 cut(s) | 488 |
| BsaBI | GATNNNNATC | 1 cut(s) | 512 |
| BsaWI | WCCGGW | 1 cut(s) | 976 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 105, 108, 135, 138 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 827, 875, 976 |
| Bse8I | GATNNNNATC | 1 cut(s) | 512 |
| BseAI | TCCGGA | 1 cut(s) | 976 |
| BseBI | CCWGG | 1 cut(s) | 870 |
| BseJI | GATNNNNATC | 1 cut(s) | 512 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 827, 875, 976 |
| BseRI | GAGGAG | 1 cut(s) | 129 |
| BseXI | GCAGC | 5 cut(s) | 66, 542, 618, 670, 1068 |
| BseYI | CCCAGC | 1 cut(s) | 462 |
| BsgI | GTGCAG | 2 cut(s) | 541, 615 |
| Bsh1236I | CGCG | 1 cut(s) | 650 |
| BsiSI | CCGG | 1 cut(s) | 977 |
| BslFI | GGGAC | 4 cut(s) | 151, 327, 445, 466 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 827, 875, 976 |
| BsmAI | GTCTC | 1 cut(s) | 557 |
| BsmFI | GGGAC | 4 cut(s) | 151, 327, 445, 466 |
| BsmI | GAATGC | 1 cut(s) | 326 |
| Bsp13I | TCCGGA | 1 cut(s) | 976 |
| Bsp1407I | TGTACA | 1 cut(s) | 657 |
| Bsp143I | GATC | 5 cut(s) | 131, 154, 469, 664, 932 |
| BspACI | CCGC | 3 cut(s) | 373, 650, 1054 |
| BspEI | TCCGGA | 1 cut(s) | 976 |
| BspFNI | CGCG | 1 cut(s) | 650 |
| BspLI | GGNNCC | 1 cut(s) | 819 |
| BspMI | ACCTGC | 1 cut(s) | 902 |
| BspPI | GGATC | 1 cut(s) | 672 |
| BsrGI | TGTACA | 1 cut(s) | 657 |
| BssMI | GATC | 5 cut(s) | 131, 154, 469, 664, 932 |
| Bst2UI | CCWGG | 1 cut(s) | 870 |
| Bst4CI | ACNGT | 2 cut(s) | 674, 844 |
| Bst6I | CTCTTC | 1 cut(s) | 424 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 863 |
| BstAUI | TGTACA | 1 cut(s) | 657 |
| BstC8I | GCNNGC | 1 cut(s) | 27 |
| BstFNI | CGCG | 1 cut(s) | 650 |
| BstKTI | GATC | 5 cut(s) | 134, 157, 472, 667, 935 |
| BstMAI | GTCTC | 1 cut(s) | 557 |
| BstMBI | GATC | 5 cut(s) | 131, 154, 469, 664, 932 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 26, 270, 359, 552, 863 |
| BstNI | CCWGG | 1 cut(s) | 870 |
| BstNSI | RCATGY | 1 cut(s) | 737 |
| BstSCI | CCNGG | 1 cut(s) | 868 |
| BstSFI | CTRYAG | 1 cut(s) | 670 |
| BstUI | CGCG | 1 cut(s) | 650 |
| BstV1I | GCAGC | 5 cut(s) | 66, 542, 618, 670, 1068 |
| BstV2I | GAAGAC | 1 cut(s) | 1092 |
| BstX2I | RGATCY | 3 cut(s) | 154, 469, 664 |
| BstYI | RGATCY | 3 cut(s) | 154, 469, 664 |
| BsuI | GTATCC | 1 cut(s) | 1061 |
| BtsIMutI | CAGTG | 2 cut(s) | 212, 812 |
| BveI | ACCTGC | 1 cut(s) | 902 |
| Cac8I | GCNNGC | 1 cut(s) | 27 |
| Cfr13I | GGNCC | 1 cut(s) | 788 |
| CseI | GACGC | 1 cut(s) | 351 |
| Csp6I | GTAC | 5 cut(s) | 128, 243, 658, 767, 1038 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 813, 848 |
| CviAII | CATG | 5 cut(s) | 593, 730, 734, 742, 991 |
| CviQI | GTAC | 5 cut(s) | 128, 243, 658, 767, 1038 |
| DpnI | GATC | 5 cut(s) | 133, 156, 471, 666, 934 |
| DpnII | GATC | 5 cut(s) | 131, 154, 469, 664, 932 |
| DriI | GACNNNNNGTC | 1 cut(s) | 751 |
| Eam1104I | CTCTTC | 1 cut(s) | 424 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 751 |
| EarI | CTCTTC | 1 cut(s) | 424 |
| Eco47I | GGWCC | 1 cut(s) | 788 |
| Eco57I | CTGAAG | 2 cut(s) | 755, 788 |
| EcoRII | CCWGG | 1 cut(s) | 868 |
| EcoT22I | ATGCAT | 2 cut(s) | 735, 743 |
| FaeI | CATG | 5 cut(s) | 596, 733, 737, 745, 994 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 64, 96 |
| FaqI | GGGAC | 4 cut(s) | 151, 327, 445, 466 |
| FatI | CATG | 5 cut(s) | 592, 729, 733, 741, 990 |
| FauI | CCCGC | 1 cut(s) | 643 |
| Fnu4HI | GCNGC | 6 cut(s) | 55, 373, 556, 607, 684, 1057 |
| Fsp4HI | GCNGC | 6 cut(s) | 55, 373, 556, 607, 684, 1057 |
| FspBI | CTAG | 4 cut(s) | 152, 300, 467, 1061 |
| GluI | GCNGC | 6 cut(s) | 55, 373, 556, 607, 684, 1057 |
| GsaI | CCCAGC | 1 cut(s) | 466 |
| GsuI | CTGGAG | 2 cut(s) | 375, 852 |
| HapII | CCGG | 1 cut(s) | 977 |
| HgaI | GACGC | 1 cut(s) | 351 |
| Hin1II | CATG | 5 cut(s) | 596, 733, 737, 745, 994 |
| HindIII | AAGCTT | 1 cut(s) | 45 |
| HinfI | GANTC | 3 cut(s) | 752, 946, 952 |
| HpaII | CCGG | 1 cut(s) | 977 |
| HphI | GGTGA | 2 cut(s) | 16, 181 |
| Hpy166II | GTNNAC | 1 cut(s) | 658 |
| Hpy188I | TCNGA | 6 cut(s) | 338, 784, 940, 951, 957, 1020 |
| Hpy188III | TCNNGA | 6 cut(s) | 161, 300, 476, 505, 977, 1001 |
| Hpy8I | GTNNAC | 1 cut(s) | 658 |
| Hpy99I | CGWCG | 3 cut(s) | 367, 910, 940 |
| HpyAV | CCTTC | 4 cut(s) | 90, 92, 870, 976 |
| HpyCH4III | ACNGT | 2 cut(s) | 674, 844 |
| HpyCH4IV | ACGT | 4 cut(s) | 140, 455, 709, 926 |
| HpyCH4V | TGCA | 7 cut(s) | 492, 558, 606, 632, 642, 733, 741 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 26, 270, 359, 552, 863 |
| HpySE526I | ACGT | 4 cut(s) | 140, 455, 709, 926 |
| Hsp92II | CATG | 5 cut(s) | 596, 733, 737, 745, 994 |
| Kpn2I | TCCGGA | 1 cut(s) | 976 |
| Kzo9I | GATC | 5 cut(s) | 131, 154, 469, 664, 932 |
| LmnI | GCTCC | 1 cut(s) | 871 |
| Lsp1109I | GCAGC | 5 cut(s) | 66, 542, 618, 670, 1068 |
| MaeI | CTAG | 4 cut(s) | 152, 300, 467, 1061 |
| MaeII | ACGT | 4 cut(s) | 140, 455, 709, 926 |
| MaeIII | GTNAC | 6 cut(s) | 179, 209, 254, 287, 674, 914 |
| MalI | GATC | 5 cut(s) | 133, 156, 471, 666, 934 |
| MboI | GATC | 5 cut(s) | 131, 154, 469, 664, 932 |
| MfeI | CAATTG | 1 cut(s) | 282 |
| MflI | RGATCY | 3 cut(s) | 154, 469, 664 |
| MluCI | AATT | 3 cut(s) | 282, 698, 847 |
| MlyI | GAGTC | 1 cut(s) | 761 |
| MmeI | TCCRAC | 2 cut(s) | 856, 918 |
| MnlI | CCTC | 4 cut(s) | 41, 107, 375, 406 |
| Mph1103I | ATGCAT | 2 cut(s) | 735, 743 |
| MroI | TCCGGA | 1 cut(s) | 976 |
| MroXI | GAANNNNTTC | 1 cut(s) | 986 |
| MspI | CCGG | 1 cut(s) | 977 |
| MspR9I | CCNGG | 1 cut(s) | 870 |
| MunI | CAATTG | 1 cut(s) | 282 |
| Mva1269I | GAATGC | 1 cut(s) | 326 |
| MvaI | CCWGG | 1 cut(s) | 870 |
| MvnI | CGCG | 1 cut(s) | 650 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 26, 270, 359, 552, 863 |
| NdeII | GATC | 5 cut(s) | 131, 154, 469, 664, 932 |
| NlaIII | CATG | 5 cut(s) | 596, 733, 737, 745, 994 |
| NlaIV | GGNNCC | 1 cut(s) | 819 |
| NmeAIII | GCCGAG | 1 cut(s) | 46 |
| NmuCI | GTSAC | 2 cut(s) | 209, 674 |
| NsiI | ATGCAT | 2 cut(s) | 735, 743 |
| NspI | RCATGY | 1 cut(s) | 737 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 932 |
| PctI | GAATGC | 1 cut(s) | 326 |
| PdmI | GAANNNNTTC | 1 cut(s) | 986 |
| PfeI | GAWTC | 2 cut(s) | 946, 952 |
| PflFI | GACNNNGTC | 1 cut(s) | 457 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 875 |
| PfoI | TCCNGGA | 1 cut(s) | 868 |
| PkrI | GCNGC | 6 cut(s) | 56, 374, 557, 608, 685, 1058 |
| PleI | GAGTC | 1 cut(s) | 760 |
| PpsI | GAGTC | 1 cut(s) | 760 |
| Psp6I | CCWGG | 1 cut(s) | 868 |
| PspFI | CCCAGC | 1 cut(s) | 462 |
| PspGI | CCWGG | 1 cut(s) | 868 |
| PspN4I | GGNNCC | 1 cut(s) | 819 |
| PspPI | GGNCC | 1 cut(s) | 788 |
| PsuI | RGATCY | 3 cut(s) | 154, 469, 664 |
| PsyI | GACNNNGTC | 1 cut(s) | 457 |
| RsaI | GTAC | 5 cut(s) | 129, 244, 659, 768, 1039 |
| RsaNI | GTAC | 5 cut(s) | 128, 243, 658, 767, 1038 |
| SatI | GCNGC | 6 cut(s) | 55, 373, 556, 607, 684, 1057 |
| Sau3AI | GATC | 5 cut(s) | 131, 154, 469, 664, 932 |
| Sau96I | GGNCC | 1 cut(s) | 788 |
| SchI | GAGTC | 1 cut(s) | 761 |
| ScrFI | CCNGG | 1 cut(s) | 870 |
| SfcI | CTRYAG | 1 cut(s) | 670 |
| SinI | GGWCC | 1 cut(s) | 788 |
| SmlI | CTYRAG | 1 cut(s) | 503 |
| SmoI | CTYRAG | 1 cut(s) | 503 |
| Sse9I | AATT | 3 cut(s) | 282, 698, 847 |
| SsiI | CCGC | 3 cut(s) | 373, 650, 1054 |
| SspI | AATATT | 1 cut(s) | 779 |
| SspMI | CTAG | 4 cut(s) | 152, 300, 467, 1061 |
| StyD4I | CCNGG | 1 cut(s) | 868 |
| TaaI | ACNGT | 2 cut(s) | 674, 844 |
| TaiI | ACGT | 4 cut(s) | 143, 458, 712, 929 |
| TaqI | TCGA | 8 cut(s) | 50, 147, 162, 365, 477, 750, 755, 905 |
| TaqII | GACCGA | 1 cut(s) | 285 |
| TasI | AATT | 3 cut(s) | 282, 698, 847 |
| TatI | WGTACW | 2 cut(s) | 242, 657 |
| TauI | GCSGC | 1 cut(s) | 375 |
| TfiI | GAWTC | 2 cut(s) | 946, 952 |
| TscAI | CASTG | 2 cut(s) | 212, 812 |
| TseFI | GTSAC | 2 cut(s) | 209, 674 |
| TseI | GCWGC | 5 cut(s) | 54, 555, 606, 683, 1056 |
| Tsp45I | GTSAC | 2 cut(s) | 209, 674 |
| TspDTI | ATGAA | 4 cut(s) | 319, 420, 959, 979 |
| TspRI | CASTG | 2 cut(s) | 212, 812 |
| Tth111I | GACNNNGTC | 1 cut(s) | 457 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 875 |
| VpaK11BI | GGWCC | 1 cut(s) | 788 |
| XbaI | TCTAGA | 1 cut(s) | 299 |
| XceI | RCATGY | 1 cut(s) | 737 |
| XmnI | GAANNNNTTC | 1 cut(s) | 986 |
| XspI | CTAG | 4 cut(s) | 152, 300, 467, 1061 |
| Zsp2I | ATGCAT | 2 cut(s) | 735, 743 |