E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 16 |
| AccII | CGCG | 1 cut(s) | 816 |
| AciI | CCGC | 1 cut(s) | 839 |
| AclWI | GGATC | 2 cut(s) | 154, 1027 |
| AcsI | RAATTY | 2 cut(s) | 239, 713 |
| AcuI | CTGAAG | 1 cut(s) | 95 |
| AfaI | GTAC | 3 cut(s) | 72, 529, 722 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 638, 717 |
| AgsI | TTSAA | 6 cut(s) | 35, 380, 748, 776, 860, 1082 |
| AjnI | CCWGG | 3 cut(s) | 110, 349, 516 |
| AluBI | AGCT | 6 cut(s) | 428, 444, 477, 867, 1033, 1045 |
| AluI | AGCT | 6 cut(s) | 428, 444, 477, 867, 1033, 1045 |
| AlwI | GGATC | 2 cut(s) | 154, 1027 |
| AoxI | GGCC | 2 cut(s) | 967, 1055 |
| ApeKI | GCWGC | 3 cut(s) | 441, 558, 642 |
| ApoI | RAATTY | 2 cut(s) | 239, 713 |
| AseI | ATTAAT | 1 cut(s) | 330 |
| AspS9I | GGNCC | 2 cut(s) | 514, 968 |
| AsuC2I | CCSGG | 1 cut(s) | 68 |
| AsuHPI | GGTGA | 5 cut(s) | 16, 38, 328, 329, 853 |
| AvaII | GGWCC | 1 cut(s) | 514 |
| AxyI | CCTNAGG | 1 cut(s) | 1059 |
| BaeGI | GKGCMC | 1 cut(s) | 423 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 712, 745 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 866, 898 |
| BbsI | GAAGAC | 1 cut(s) | 848 |
| BbvI | GCAGC | 3 cut(s) | 453, 545, 629 |
| BccI | CCATC | 6 cut(s) | 137, 320, 398, 472, 959, 1046 |
| BciT130I | CCWGG | 3 cut(s) | 112, 351, 518 |
| BcnI | CCSGG | 1 cut(s) | 68 |
| BfaI | CTAG | 6 cut(s) | 182, 209, 474, 489, 663, 864 |
| BfmI | CTRYAG | 1 cut(s) | 439 |
| BisI | GCNGC | 3 cut(s) | 442, 559, 643 |
| BlsI | GCNGC | 3 cut(s) | 443, 560, 644 |
| Bme1390I | CCNGG | 4 cut(s) | 68, 112, 351, 518 |
| Bme18I | GGWCC | 1 cut(s) | 514 |
| BmgT120I | GGNCC | 2 cut(s) | 514, 968 |
| BmiI | GGNNCC | 1 cut(s) | 58 |
| BmrFI | CCNGG | 4 cut(s) | 68, 112, 351, 518 |
| BmrI | ACTGGG | 1 cut(s) | 389 |
| BmuI | ACTGGG | 1 cut(s) | 389 |
| BpiI | GAAGAC | 1 cut(s) | 848 |
| BpuEI | CTTGAG | 1 cut(s) | 791 |
| BpuMI | CCSGG | 1 cut(s) | 68 |
| BsaJI | CCNNGG | 2 cut(s) | 111, 349 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 428, 458 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 638, 717 |
| Bse1I | ACTGG | 2 cut(s) | 395, 403 |
| Bse21I | CCTNAGG | 1 cut(s) | 1059 |
| BseBI | CCWGG | 3 cut(s) | 112, 351, 518 |
| BseDI | CCNNGG | 2 cut(s) | 111, 349 |
| BseGI | GGATG | 2 cut(s) | 178, 390 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 638, 717 |
| BseMII | CTCAG | 1 cut(s) | 934 |
| BseNI | ACTGG | 2 cut(s) | 395, 403 |
| BseRI | GAGGAG | 1 cut(s) | 143 |
| BseSI | GKGCMC | 1 cut(s) | 423 |
| BseXI | GCAGC | 3 cut(s) | 453, 545, 629 |
| Bsh1236I | CGCG | 1 cut(s) | 816 |
| BshFI | GGCC | 2 cut(s) | 969, 1057 |
| BsiSI | CCGG | 1 cut(s) | 68 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 638, 717 |
| BsmI | GAATGC | 1 cut(s) | 841 |
| BsnI | GGCC | 2 cut(s) | 969, 1057 |
| Bsp1286I | GDGCHC | 1 cut(s) | 423 |
| Bsp143I | GATC | 2 cut(s) | 146, 1019 |
| BspACI | CCGC | 1 cut(s) | 839 |
| BspANI | GGCC | 2 cut(s) | 969, 1057 |
| BspCNI | CTCAG | 1 cut(s) | 933 |
| BspFNI | CGCG | 1 cut(s) | 816 |
| BspHI | TCATGA | 1 cut(s) | 870 |
| BspLI | GGNNCC | 1 cut(s) | 58 |
| BspMAI | CTGCAG | 1 cut(s) | 443 |
| BspPI | GGATC | 2 cut(s) | 154, 1027 |
| BspQI | GCTCTTC | 1 cut(s) | 288 |
| BsrI | ACTGG | 2 cut(s) | 395, 403 |
| BssECI | CCNNGG | 2 cut(s) | 111, 349 |
| BssMI | GATC | 2 cut(s) | 146, 1019 |
| Bst2UI | CCWGG | 3 cut(s) | 112, 351, 518 |
| Bst4CI | ACNGT | 3 cut(s) | 728, 769, 930 |
| Bst6I | CTCTTC | 4 cut(s) | 71, 162, 282, 288 |
| BstC8I | GCNNGC | 3 cut(s) | 430, 607, 611 |
| BstDEI | CTNAG | 3 cut(s) | 920, 939, 1059 |
| BstF5I | GGATG | 2 cut(s) | 178, 390 |
| BstFNI | CGCG | 1 cut(s) | 816 |
| BstKTI | GATC | 2 cut(s) | 149, 1022 |
| BstMBI | GATC | 2 cut(s) | 146, 1019 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 438, 483, 555 |
| BstNI | CCWGG | 3 cut(s) | 112, 351, 518 |
| BstNSI | RCATGY | 2 cut(s) | 609, 613 |
| BstSCI | CCNGG | 4 cut(s) | 66, 110, 349, 516 |
| BstSFI | CTRYAG | 1 cut(s) | 439 |
| BstSLI | GKGCMC | 1 cut(s) | 423 |
| BstUI | CGCG | 1 cut(s) | 816 |
| BstV1I | GCAGC | 3 cut(s) | 453, 545, 629 |
| BstV2I | GAAGAC | 1 cut(s) | 848 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1059 |
| BsuRI | GGCC | 2 cut(s) | 969, 1057 |
| BtsCI | GGATG | 2 cut(s) | 178, 390 |
| BtsIMutI | CAGTG | 2 cut(s) | 402, 410 |
| Cac8I | GCNNGC | 3 cut(s) | 430, 607, 611 |
| CciI | TCATGA | 1 cut(s) | 870 |
| Cfr13I | GGNCC | 2 cut(s) | 514, 968 |
| CseI | GACGC | 1 cut(s) | 805 |
| Csp6I | GTAC | 3 cut(s) | 71, 528, 721 |
| CviQI | GTAC | 3 cut(s) | 71, 528, 721 |
| DdeI | CTNAG | 3 cut(s) | 920, 939, 1059 |
| DpnI | GATC | 2 cut(s) | 148, 1021 |
| DpnII | GATC | 2 cut(s) | 146, 1019 |
| Eam1104I | CTCTTC | 4 cut(s) | 71, 162, 282, 288 |
| EarI | CTCTTC | 4 cut(s) | 71, 162, 282, 288 |
| Eco32I | GATATC | 1 cut(s) | 83 |
| Eco47I | GGWCC | 1 cut(s) | 514 |
| Eco57I | CTGAAG | 1 cut(s) | 95 |
| Eco81I | CCTNAGG | 1 cut(s) | 1059 |
| EcoO109I | RGGNCCY | 1 cut(s) | 514 |
| EcoRII | CCWGG | 3 cut(s) | 110, 349, 516 |
| EcoRV | GATATC | 1 cut(s) | 83 |
| EcoT22I | ATGCAT | 2 cut(s) | 611, 615 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 517, 549 |
| FblI | GTMKAC | 1 cut(s) | 16 |
| Fnu4HI | GCNGC | 3 cut(s) | 442, 559, 643 |
| FokI | GGATG | 2 cut(s) | 185, 377 |
| Fsp4HI | GCNGC | 3 cut(s) | 442, 559, 643 |
| FspBI | CTAG | 6 cut(s) | 182, 209, 474, 489, 663, 864 |
| GluI | GCNGC | 3 cut(s) | 442, 559, 643 |
| HaeIII | GGCC | 2 cut(s) | 969, 1057 |
| HapII | CCGG | 1 cut(s) | 68 |
| HgaI | GACGC | 1 cut(s) | 805 |
| HincII | GTYRAC | 1 cut(s) | 17 |
| HindII | GTYRAC | 1 cut(s) | 17 |
| HinfI | GANTC | 3 cut(s) | 521, 811, 878 |
| HpaII | CCGG | 1 cut(s) | 68 |
| HphI | GGTGA | 5 cut(s) | 16, 38, 328, 329, 853 |
| Hpy166II | GTNNAC | 2 cut(s) | 17, 765 |
| Hpy188I | TCNGA | 3 cut(s) | 151, 923, 1019 |
| Hpy188III | TCNNGA | 7 cut(s) | 89, 164, 191, 218, 776, 808, 871 |
| Hpy8I | GTNNAC | 2 cut(s) | 17, 765 |
| Hpy99I | CGWCG | 1 cut(s) | 90 |
| HpyAV | CCTTC | 8 cut(s) | 38, 108, 110, 505, 782, 818, 868, 984 |
| HpyCH4III | ACNGT | 3 cut(s) | 728, 769, 930 |
| HpyCH4V | TGCA | 4 cut(s) | 441, 561, 609, 613 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 438, 483, 555 |
| HpyF3I | CTNAG | 3 cut(s) | 920, 939, 1059 |
| Kzo9I | GATC | 2 cut(s) | 146, 1019 |
| LguI | GCTCTTC | 1 cut(s) | 288 |
| LmnI | GCTCC | 2 cut(s) | 449, 482 |
| Lsp1109I | GCAGC | 3 cut(s) | 453, 545, 629 |
| MaeI | CTAG | 6 cut(s) | 182, 209, 474, 489, 663, 864 |
| MaeIII | GTNAC | 1 cut(s) | 334 |
| MalI | GATC | 2 cut(s) | 148, 1021 |
| MboI | GATC | 2 cut(s) | 146, 1019 |
| MfeI | CAATTG | 1 cut(s) | 1077 |
| MhlI | GDGCHC | 1 cut(s) | 423 |
| MluCI | AATT | 6 cut(s) | 239, 301, 369, 713, 850, 1077 |
| MlyI | GAGTC | 1 cut(s) | 887 |
| MmeI | TCCRAC | 1 cut(s) | 31 |
| Mph1103I | ATGCAT | 2 cut(s) | 611, 615 |
| MseI | TTAA | 6 cut(s) | 330, 341, 386, 849, 900, 998 |
| MslI | CAYNNNNRTG | 1 cut(s) | 395 |
| MspI | CCGG | 1 cut(s) | 68 |
| MspR9I | CCNGG | 4 cut(s) | 68, 112, 351, 518 |
| MunI | CAATTG | 1 cut(s) | 1077 |
| Mva1269I | GAATGC | 1 cut(s) | 841 |
| MvaI | CCWGG | 3 cut(s) | 112, 351, 518 |
| MvnI | CGCG | 1 cut(s) | 816 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 438, 483, 555 |
| NciI | CCSGG | 1 cut(s) | 68 |
| NdeII | GATC | 2 cut(s) | 146, 1019 |
| NlaIV | GGNNCC | 1 cut(s) | 58 |
| NmuCI | GTSAC | 1 cut(s) | 334 |
| NsiI | ATGCAT | 2 cut(s) | 611, 615 |
| NspI | RCATGY | 2 cut(s) | 609, 613 |
| PaeI | GCATGC | 2 cut(s) | 609, 613 |
| PagI | TCATGA | 1 cut(s) | 870 |
| PciSI | GCTCTTC | 1 cut(s) | 288 |
| PctI | GAATGC | 1 cut(s) | 841 |
| PfeI | GAWTC | 2 cut(s) | 521, 811 |
| PflFI | GACNNNGTC | 2 cut(s) | 12, 650 |
| PfoI | TCCNGGA | 1 cut(s) | 516 |
| PkrI | GCNGC | 3 cut(s) | 443, 560, 644 |
| PleI | GAGTC | 1 cut(s) | 886 |
| PpsI | GAGTC | 1 cut(s) | 886 |
| PpuMI | RGGWCCY | 1 cut(s) | 514 |
| PshBI | ATTAAT | 1 cut(s) | 330 |
| Psp5II | RGGWCCY | 1 cut(s) | 514 |
| Psp6I | CCWGG | 3 cut(s) | 110, 349, 516 |
| PspGI | CCWGG | 3 cut(s) | 110, 349, 516 |
| PspN4I | GGNNCC | 1 cut(s) | 58 |
| PspPI | GGNCC | 2 cut(s) | 514, 968 |
| PspPPI | RGGWCCY | 1 cut(s) | 514 |
| PstI | CTGCAG | 1 cut(s) | 443 |
| PsyI | GACNNNGTC | 2 cut(s) | 12, 650 |
| RsaI | GTAC | 3 cut(s) | 72, 529, 722 |
| RsaNI | GTAC | 3 cut(s) | 71, 528, 721 |
| RseI | CAYNNNNRTG | 1 cut(s) | 395 |
| SalI | GTCGAC | 1 cut(s) | 15 |
| SapI | GCTCTTC | 1 cut(s) | 288 |
| SaqAI | TTAA | 6 cut(s) | 330, 341, 386, 849, 900, 998 |
| SatI | GCNGC | 3 cut(s) | 442, 559, 643 |
| Sau3AI | GATC | 2 cut(s) | 146, 1019 |
| Sau96I | GGNCC | 2 cut(s) | 514, 968 |
| SchI | GAGTC | 1 cut(s) | 887 |
| ScrFI | CCNGG | 4 cut(s) | 68, 112, 351, 518 |
| SduI | GDGCHC | 1 cut(s) | 423 |
| SfcI | CTRYAG | 1 cut(s) | 439 |
| SinI | GGWCC | 1 cut(s) | 514 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 395 |
| SmlI | CTYRAG | 1 cut(s) | 806 |
| SmoI | CTYRAG | 1 cut(s) | 806 |
| SphI | GCATGC | 2 cut(s) | 609, 613 |
| Sse9I | AATT | 6 cut(s) | 239, 301, 369, 713, 850, 1077 |
| SsiI | CCGC | 1 cut(s) | 839 |
| SspMI | CTAG | 6 cut(s) | 182, 209, 474, 489, 663, 864 |
| StyD4I | CCNGG | 4 cut(s) | 66, 110, 349, 516 |
| TaaI | ACNGT | 3 cut(s) | 728, 769, 930 |
| TaqI | TCGA | 6 cut(s) | 16, 88, 279, 541, 822, 1008 |
| TasI | AATT | 6 cut(s) | 239, 301, 369, 713, 850, 1077 |
| TatI | WGTACW | 1 cut(s) | 527 |
| TfiI | GAWTC | 2 cut(s) | 521, 811 |
| Tru1I | TTAA | 6 cut(s) | 330, 341, 386, 849, 900, 998 |
| Tru9I | TTAA | 6 cut(s) | 330, 341, 386, 849, 900, 998 |
| TscAI | CASTG | 2 cut(s) | 402, 410 |
| TseFI | GTSAC | 1 cut(s) | 334 |
| TseI | GCWGC | 3 cut(s) | 441, 558, 642 |
| Tsp45I | GTSAC | 1 cut(s) | 334 |
| TspGWI | ACGGA | 1 cut(s) | 924 |
| TspRI | CASTG | 2 cut(s) | 402, 410 |
| Tth111I | GACNNNGTC | 2 cut(s) | 12, 650 |
| VpaK11BI | GGWCC | 1 cut(s) | 514 |
| VspI | ATTAAT | 1 cut(s) | 330 |
| XapI | RAATTY | 2 cut(s) | 239, 713 |
| XceI | RCATGY | 2 cut(s) | 609, 613 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 962 |
| XmiI | GTMKAC | 1 cut(s) | 16 |
| XspI | CTAG | 6 cut(s) | 182, 209, 474, 489, 663, 864 |
| Zsp2I | ATGCAT | 2 cut(s) | 611, 615 |