E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 16 |
| AccII | CGCG | 1 cut(s) | 789 |
| AciI | CCGC | 1 cut(s) | 812 |
| AclWI | GGATC | 2 cut(s) | 154, 1000 |
| AcsI | RAATTY | 2 cut(s) | 212, 686 |
| AcuI | CTGAAG | 1 cut(s) | 95 |
| AfaI | GTAC | 3 cut(s) | 72, 502, 695 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 611, 690 |
| AgsI | TTSAA | 6 cut(s) | 35, 353, 721, 749, 833, 1055 |
| AjnI | CCWGG | 3 cut(s) | 110, 322, 489 |
| AluBI | AGCT | 4 cut(s) | 401, 417, 840, 1006 |
| AluI | AGCT | 4 cut(s) | 401, 417, 840, 1006 |
| AlwI | GGATC | 2 cut(s) | 154, 1000 |
| AoxI | GGCC | 2 cut(s) | 940, 1028 |
| ApeKI | GCWGC | 3 cut(s) | 414, 531, 615 |
| ApoI | RAATTY | 2 cut(s) | 212, 686 |
| AseI | ATTAAT | 1 cut(s) | 303 |
| AspS9I | GGNCC | 2 cut(s) | 487, 941 |
| AsuC2I | CCSGG | 1 cut(s) | 68 |
| AsuHPI | GGTGA | 6 cut(s) | 16, 38, 185, 301, 302, 826 |
| AvaII | GGWCC | 1 cut(s) | 487 |
| AxyI | CCTNAGG | 1 cut(s) | 1032 |
| BaeGI | GKGCMC | 1 cut(s) | 396 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 685, 718 |
| BanII | GRGCYC | 1 cut(s) | 894 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 839, 871 |
| BbsI | GAAGAC | 1 cut(s) | 821 |
| BbvI | GCAGC | 3 cut(s) | 426, 518, 602 |
| BccI | CCATC | 6 cut(s) | 137, 164, 371, 932, 949, 1019 |
| BciT130I | CCWGG | 3 cut(s) | 112, 324, 491 |
| BcnI | CCSGG | 1 cut(s) | 68 |
| BfaI | CTAG | 4 cut(s) | 182, 447, 462, 837 |
| BfmI | CTRYAG | 1 cut(s) | 412 |
| BisI | GCNGC | 3 cut(s) | 415, 532, 616 |
| BlsI | GCNGC | 3 cut(s) | 416, 533, 617 |
| BmcAI | AGTACT | 1 cut(s) | 502 |
| Bme1390I | CCNGG | 4 cut(s) | 68, 112, 324, 491 |
| Bme18I | GGWCC | 1 cut(s) | 487 |
| BmgT120I | GGNCC | 2 cut(s) | 487, 941 |
| BmrFI | CCNGG | 4 cut(s) | 68, 112, 324, 491 |
| BmrI | ACTGGG | 1 cut(s) | 362 |
| BmsI | GCATC | 1 cut(s) | 217 |
| BmuI | ACTGGG | 1 cut(s) | 362 |
| BpiI | GAAGAC | 1 cut(s) | 821 |
| BpuEI | CTTGAG | 1 cut(s) | 764 |
| BpuMI | CCSGG | 1 cut(s) | 68 |
| BsaJI | CCNNGG | 2 cut(s) | 111, 322 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 401, 431 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 611, 690 |
| Bse1I | ACTGG | 2 cut(s) | 368, 376 |
| Bse21I | CCTNAGG | 1 cut(s) | 1032 |
| BseBI | CCWGG | 3 cut(s) | 112, 324, 491 |
| BseDI | CCNNGG | 2 cut(s) | 111, 322 |
| BseGI | GGATG | 1 cut(s) | 363 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 611, 690 |
| BseMII | CTCAG | 1 cut(s) | 907 |
| BseNI | ACTGG | 2 cut(s) | 368, 376 |
| BseRI | GAGGAG | 1 cut(s) | 143 |
| BseSI | GKGCMC | 1 cut(s) | 396 |
| BseXI | GCAGC | 3 cut(s) | 426, 518, 602 |
| Bsh1236I | CGCG | 1 cut(s) | 789 |
| BshFI | GGCC | 2 cut(s) | 942, 1030 |
| BsiSI | CCGG | 1 cut(s) | 68 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 611, 690 |
| BsmI | GAATGC | 1 cut(s) | 814 |
| BsnI | GGCC | 2 cut(s) | 942, 1030 |
| Bsp1286I | GDGCHC | 2 cut(s) | 396, 894 |
| Bsp143I | GATC | 2 cut(s) | 146, 992 |
| BspACI | CCGC | 1 cut(s) | 812 |
| BspANI | GGCC | 2 cut(s) | 942, 1030 |
| BspCNI | CTCAG | 1 cut(s) | 906 |
| BspFNI | CGCG | 1 cut(s) | 789 |
| BspHI | TCATGA | 1 cut(s) | 843 |
| BspMAI | CTGCAG | 1 cut(s) | 416 |
| BspPI | GGATC | 2 cut(s) | 154, 1000 |
| BspQI | GCTCTTC | 1 cut(s) | 261 |
| BsrI | ACTGG | 2 cut(s) | 368, 376 |
| BssECI | CCNNGG | 2 cut(s) | 111, 322 |
| BssMI | GATC | 2 cut(s) | 146, 992 |
| Bst2UI | CCWGG | 3 cut(s) | 112, 324, 491 |
| Bst4CI | ACNGT | 3 cut(s) | 701, 742, 903 |
| Bst6I | CTCTTC | 3 cut(s) | 71, 255, 261 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 227, 924 |
| BstC8I | GCNNGC | 3 cut(s) | 403, 580, 584 |
| BstDEI | CTNAG | 3 cut(s) | 893, 912, 1032 |
| BstF5I | GGATG | 1 cut(s) | 363 |
| BstFNI | CGCG | 1 cut(s) | 789 |
| BstKTI | GATC | 2 cut(s) | 149, 995 |
| BstMBI | GATC | 2 cut(s) | 146, 992 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 227, 411, 528, 924 |
| BstNI | CCWGG | 3 cut(s) | 112, 324, 491 |
| BstNSI | RCATGY | 2 cut(s) | 582, 586 |
| BstSCI | CCNGG | 4 cut(s) | 66, 110, 322, 489 |
| BstSFI | CTRYAG | 1 cut(s) | 412 |
| BstSLI | GKGCMC | 1 cut(s) | 396 |
| BstUI | CGCG | 1 cut(s) | 789 |
| BstV1I | GCAGC | 3 cut(s) | 426, 518, 602 |
| BstV2I | GAAGAC | 1 cut(s) | 821 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1032 |
| BsuRI | GGCC | 2 cut(s) | 942, 1030 |
| BtsCI | GGATG | 1 cut(s) | 363 |
| BtsIMutI | CAGTG | 2 cut(s) | 375, 383 |
| Cac8I | GCNNGC | 3 cut(s) | 403, 580, 584 |
| CciI | TCATGA | 1 cut(s) | 843 |
| Cfr13I | GGNCC | 2 cut(s) | 487, 941 |
| CseI | GACGC | 1 cut(s) | 778 |
| Csp6I | GTAC | 3 cut(s) | 71, 501, 694 |
| CviQI | GTAC | 3 cut(s) | 71, 501, 694 |
| DdeI | CTNAG | 3 cut(s) | 893, 912, 1032 |
| DpnI | GATC | 2 cut(s) | 148, 994 |
| DpnII | GATC | 2 cut(s) | 146, 992 |
| Eam1104I | CTCTTC | 3 cut(s) | 71, 255, 261 |
| EarI | CTCTTC | 3 cut(s) | 71, 255, 261 |
| Eco24I | GRGCYC | 1 cut(s) | 894 |
| Eco32I | GATATC | 1 cut(s) | 83 |
| Eco47I | GGWCC | 1 cut(s) | 487 |
| Eco57I | CTGAAG | 1 cut(s) | 95 |
| Eco81I | CCTNAGG | 1 cut(s) | 1032 |
| EcoO109I | RGGNCCY | 1 cut(s) | 487 |
| EcoRII | CCWGG | 3 cut(s) | 110, 322, 489 |
| EcoRV | GATATC | 1 cut(s) | 83 |
| EcoT22I | ATGCAT | 2 cut(s) | 584, 588 |
| EcoT38I | GRGCYC | 1 cut(s) | 894 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 490, 522 |
| FblI | GTMKAC | 1 cut(s) | 16 |
| Fnu4HI | GCNGC | 3 cut(s) | 415, 532, 616 |
| FokI | GGATG | 1 cut(s) | 350 |
| FriOI | GRGCYC | 1 cut(s) | 894 |
| Fsp4HI | GCNGC | 3 cut(s) | 415, 532, 616 |
| FspBI | CTAG | 4 cut(s) | 182, 447, 462, 837 |
| GluI | GCNGC | 3 cut(s) | 415, 532, 616 |
| HaeIII | GGCC | 2 cut(s) | 942, 1030 |
| HapII | CCGG | 1 cut(s) | 68 |
| HgaI | GACGC | 1 cut(s) | 778 |
| HincII | GTYRAC | 1 cut(s) | 17 |
| HindII | GTYRAC | 1 cut(s) | 17 |
| HinfI | GANTC | 3 cut(s) | 494, 784, 851 |
| HpaII | CCGG | 1 cut(s) | 68 |
| HphI | GGTGA | 6 cut(s) | 16, 38, 185, 301, 302, 826 |
| Hpy166II | GTNNAC | 2 cut(s) | 17, 738 |
| Hpy188I | TCNGA | 3 cut(s) | 151, 896, 992 |
| Hpy188III | TCNNGA | 6 cut(s) | 89, 164, 191, 749, 781, 844 |
| Hpy8I | GTNNAC | 2 cut(s) | 17, 738 |
| Hpy99I | CGWCG | 1 cut(s) | 90 |
| HpyAV | CCTTC | 8 cut(s) | 38, 108, 110, 478, 755, 791, 841, 957 |
| HpyCH4III | ACNGT | 3 cut(s) | 701, 742, 903 |
| HpyCH4IV | ACGT | 1 cut(s) | 55 |
| HpyCH4V | TGCA | 4 cut(s) | 414, 534, 582, 586 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 227, 411, 528, 924 |
| HpyF3I | CTNAG | 3 cut(s) | 893, 912, 1032 |
| HpySE526I | ACGT | 1 cut(s) | 55 |
| Kzo9I | GATC | 2 cut(s) | 146, 992 |
| LguI | GCTCTTC | 1 cut(s) | 261 |
| LmnI | GCTCC | 1 cut(s) | 422 |
| Lsp1109I | GCAGC | 3 cut(s) | 426, 518, 602 |
| LweI | GCATC | 1 cut(s) | 217 |
| MaeI | CTAG | 4 cut(s) | 182, 447, 462, 837 |
| MaeII | ACGT | 1 cut(s) | 55 |
| MaeIII | GTNAC | 2 cut(s) | 307, 998 |
| MalI | GATC | 2 cut(s) | 148, 994 |
| MboI | GATC | 2 cut(s) | 146, 992 |
| MfeI | CAATTG | 1 cut(s) | 1050 |
| MhlI | GDGCHC | 2 cut(s) | 396, 894 |
| MluCI | AATT | 6 cut(s) | 212, 274, 342, 686, 823, 1050 |
| MlyI | GAGTC | 1 cut(s) | 860 |
| MmeI | TCCRAC | 1 cut(s) | 31 |
| MnlI | CCTC | 9 cut(s) | 118, 121, 193, 256, 788, 810, 954, 977, 1056 |
| Mph1103I | ATGCAT | 2 cut(s) | 584, 588 |
| MseI | TTAA | 6 cut(s) | 303, 314, 359, 822, 873, 971 |
| MslI | CAYNNNNRTG | 1 cut(s) | 368 |
| MspI | CCGG | 1 cut(s) | 68 |
| MspR9I | CCNGG | 4 cut(s) | 68, 112, 324, 491 |
| MunI | CAATTG | 1 cut(s) | 1050 |
| Mva1269I | GAATGC | 1 cut(s) | 814 |
| MvaI | CCWGG | 3 cut(s) | 112, 324, 491 |
| MvnI | CGCG | 1 cut(s) | 789 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 227, 411, 528, 924 |
| NciI | CCSGG | 1 cut(s) | 68 |
| NdeII | GATC | 2 cut(s) | 146, 992 |
| NmuCI | GTSAC | 2 cut(s) | 307, 998 |
| NsiI | ATGCAT | 2 cut(s) | 584, 588 |
| NspI | RCATGY | 2 cut(s) | 582, 586 |
| PaeI | GCATGC | 2 cut(s) | 582, 586 |
| PagI | TCATGA | 1 cut(s) | 843 |
| PciSI | GCTCTTC | 1 cut(s) | 261 |
| PctI | GAATGC | 1 cut(s) | 814 |
| PfeI | GAWTC | 2 cut(s) | 494, 784 |
| PflFI | GACNNNGTC | 2 cut(s) | 12, 623 |
| PfoI | TCCNGGA | 1 cut(s) | 489 |
| PkrI | GCNGC | 3 cut(s) | 416, 533, 617 |
| PleI | GAGTC | 1 cut(s) | 859 |
| PpsI | GAGTC | 1 cut(s) | 859 |
| PpuMI | RGGWCCY | 1 cut(s) | 487 |
| PshBI | ATTAAT | 1 cut(s) | 303 |
| Psp5II | RGGWCCY | 1 cut(s) | 487 |
| Psp6I | CCWGG | 3 cut(s) | 110, 322, 489 |
| PspGI | CCWGG | 3 cut(s) | 110, 322, 489 |
| PspPI | GGNCC | 2 cut(s) | 487, 941 |
| PspPPI | RGGWCCY | 1 cut(s) | 487 |
| PstI | CTGCAG | 1 cut(s) | 416 |
| PsyI | GACNNNGTC | 2 cut(s) | 12, 623 |
| RsaI | GTAC | 3 cut(s) | 72, 502, 695 |
| RsaNI | GTAC | 3 cut(s) | 71, 501, 694 |
| RseI | CAYNNNNRTG | 1 cut(s) | 368 |
| SalI | GTCGAC | 1 cut(s) | 15 |
| SapI | GCTCTTC | 1 cut(s) | 261 |
| SaqAI | TTAA | 6 cut(s) | 303, 314, 359, 822, 873, 971 |
| SatI | GCNGC | 3 cut(s) | 415, 532, 616 |
| Sau3AI | GATC | 2 cut(s) | 146, 992 |
| Sau96I | GGNCC | 2 cut(s) | 487, 941 |
| ScaI | AGTACT | 1 cut(s) | 502 |
| SchI | GAGTC | 1 cut(s) | 860 |
| ScrFI | CCNGG | 4 cut(s) | 68, 112, 324, 491 |
| SduI | GDGCHC | 2 cut(s) | 396, 894 |
| SfaNI | GCATC | 1 cut(s) | 217 |
| SfcI | CTRYAG | 1 cut(s) | 412 |
| SinI | GGWCC | 1 cut(s) | 487 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 368 |
| SmlI | CTYRAG | 1 cut(s) | 779 |
| SmoI | CTYRAG | 1 cut(s) | 779 |
| SphI | GCATGC | 2 cut(s) | 582, 586 |
| Sse9I | AATT | 6 cut(s) | 212, 274, 342, 686, 823, 1050 |
| SsiI | CCGC | 1 cut(s) | 812 |
| SspMI | CTAG | 4 cut(s) | 182, 447, 462, 837 |
| StyD4I | CCNGG | 4 cut(s) | 66, 110, 322, 489 |
| TaaI | ACNGT | 3 cut(s) | 701, 742, 903 |
| TaiI | ACGT | 1 cut(s) | 58 |
| TaqI | TCGA | 7 cut(s) | 16, 88, 252, 514, 795, 953, 981 |
| TasI | AATT | 6 cut(s) | 212, 274, 342, 686, 823, 1050 |
| TatI | WGTACW | 1 cut(s) | 500 |
| TfiI | GAWTC | 2 cut(s) | 494, 784 |
| Tru1I | TTAA | 6 cut(s) | 303, 314, 359, 822, 873, 971 |
| Tru9I | TTAA | 6 cut(s) | 303, 314, 359, 822, 873, 971 |
| TscAI | CASTG | 2 cut(s) | 375, 383 |
| TseFI | GTSAC | 2 cut(s) | 307, 998 |
| TseI | GCWGC | 3 cut(s) | 414, 531, 615 |
| Tsp45I | GTSAC | 2 cut(s) | 307, 998 |
| TspGWI | ACGGA | 2 cut(s) | 426, 897 |
| TspRI | CASTG | 2 cut(s) | 375, 383 |
| Tth111I | GACNNNGTC | 2 cut(s) | 12, 623 |
| VpaK11BI | GGWCC | 1 cut(s) | 487 |
| VspI | ATTAAT | 1 cut(s) | 303 |
| XapI | RAATTY | 2 cut(s) | 212, 686 |
| XceI | RCATGY | 2 cut(s) | 582, 586 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 935 |
| XmiI | GTMKAC | 1 cut(s) | 16 |
| XspI | CTAG | 4 cut(s) | 182, 447, 462, 837 |
| ZrmI | AGTACT | 1 cut(s) | 502 |
| Zsp2I | ATGCAT | 2 cut(s) | 584, 588 |