E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 16, 962 |
| AccII | CGCG | 2 cut(s) | 792, 815 |
| AciI | CCGC | 1 cut(s) | 815 |
| AclWI | GGATC | 2 cut(s) | 154, 1003 |
| AcsI | RAATTY | 2 cut(s) | 212, 689 |
| AcuI | CTGAAG | 1 cut(s) | 95 |
| AfaI | GTAC | 3 cut(s) | 72, 505, 698 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 614, 693 |
| AgsI | TTSAA | 6 cut(s) | 35, 356, 724, 752, 836, 1058 |
| AjnI | CCWGG | 3 cut(s) | 110, 325, 492 |
| AluBI | AGCT | 4 cut(s) | 404, 420, 843, 1009 |
| AluI | AGCT | 4 cut(s) | 404, 420, 843, 1009 |
| AlwI | GGATC | 2 cut(s) | 154, 1003 |
| AoxI | GGCC | 2 cut(s) | 943, 1031 |
| ApeKI | GCWGC | 3 cut(s) | 417, 534, 618 |
| ApoI | RAATTY | 2 cut(s) | 212, 689 |
| AseI | ATTAAT | 1 cut(s) | 306 |
| AspS9I | GGNCC | 2 cut(s) | 490, 944 |
| AsuC2I | CCSGG | 1 cut(s) | 68 |
| AsuHPI | GGTGA | 6 cut(s) | 16, 38, 304, 305, 716, 829 |
| AvaII | GGWCC | 1 cut(s) | 490 |
| AxyI | CCTNAGG | 1 cut(s) | 1035 |
| BaeGI | GKGCMC | 1 cut(s) | 399 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 688, 721 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 842, 874 |
| BbsI | GAAGAC | 1 cut(s) | 824 |
| BbvI | GCAGC | 3 cut(s) | 429, 521, 605 |
| BccI | CCATC | 5 cut(s) | 137, 374, 448, 935, 1022 |
| BciT130I | CCWGG | 3 cut(s) | 112, 327, 494 |
| BcnI | CCSGG | 1 cut(s) | 68 |
| BfaI | CTAG | 5 cut(s) | 182, 450, 465, 639, 840 |
| BfmI | CTRYAG | 1 cut(s) | 415 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 459 |
| BisI | GCNGC | 3 cut(s) | 418, 535, 619 |
| BlsI | GCNGC | 3 cut(s) | 419, 536, 620 |
| Bme1390I | CCNGG | 4 cut(s) | 68, 112, 327, 494 |
| Bme18I | GGWCC | 1 cut(s) | 490 |
| BmgT120I | GGNCC | 2 cut(s) | 490, 944 |
| BmiI | GGNNCC | 1 cut(s) | 58 |
| BmrFI | CCNGG | 4 cut(s) | 68, 112, 327, 494 |
| BmrI | ACTGGG | 1 cut(s) | 365 |
| BmuI | ACTGGG | 1 cut(s) | 365 |
| BpiI | GAAGAC | 1 cut(s) | 824 |
| BpuEI | CTTGAG | 1 cut(s) | 767 |
| BpuMI | CCSGG | 1 cut(s) | 68 |
| BsaJI | CCNNGG | 2 cut(s) | 111, 325 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 404, 434 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 614, 693 |
| Bse1I | ACTGG | 2 cut(s) | 371, 379 |
| Bse21I | CCTNAGG | 1 cut(s) | 1035 |
| BseBI | CCWGG | 3 cut(s) | 112, 327, 494 |
| BseDI | CCNNGG | 2 cut(s) | 111, 325 |
| BseGI | GGATG | 1 cut(s) | 366 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 614, 693 |
| BseNI | ACTGG | 2 cut(s) | 371, 379 |
| BseRI | GAGGAG | 1 cut(s) | 143 |
| BseSI | GKGCMC | 1 cut(s) | 399 |
| BseXI | GCAGC | 3 cut(s) | 429, 521, 605 |
| Bsh1236I | CGCG | 2 cut(s) | 792, 815 |
| BshFI | GGCC | 2 cut(s) | 945, 1033 |
| BsiSI | CCGG | 1 cut(s) | 68 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 614, 693 |
| BsnI | GGCC | 2 cut(s) | 945, 1033 |
| Bsp1286I | GDGCHC | 1 cut(s) | 399 |
| Bsp143I | GATC | 2 cut(s) | 146, 995 |
| BspACI | CCGC | 1 cut(s) | 815 |
| BspANI | GGCC | 2 cut(s) | 945, 1033 |
| BspFNI | CGCG | 2 cut(s) | 792, 815 |
| BspHI | TCATGA | 1 cut(s) | 846 |
| BspLI | GGNNCC | 1 cut(s) | 58 |
| BspMAI | CTGCAG | 1 cut(s) | 419 |
| BspPI | GGATC | 2 cut(s) | 154, 1003 |
| BspQI | GCTCTTC | 1 cut(s) | 261 |
| BsrI | ACTGG | 2 cut(s) | 371, 379 |
| BssECI | CCNNGG | 2 cut(s) | 111, 325 |
| BssMI | GATC | 2 cut(s) | 146, 995 |
| Bst2UI | CCWGG | 3 cut(s) | 112, 327, 494 |
| Bst4CI | ACNGT | 3 cut(s) | 704, 745, 906 |
| Bst6I | CTCTTC | 3 cut(s) | 71, 255, 261 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 927 |
| BstC8I | GCNNGC | 3 cut(s) | 406, 583, 587 |
| BstDEI | CTNAG | 2 cut(s) | 915, 1035 |
| BstF5I | GGATG | 1 cut(s) | 366 |
| BstFNI | CGCG | 2 cut(s) | 792, 815 |
| BstKTI | GATC | 2 cut(s) | 149, 998 |
| BstMBI | GATC | 2 cut(s) | 146, 995 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 414, 459, 531, 927 |
| BstNI | CCWGG | 3 cut(s) | 112, 327, 494 |
| BstNSI | RCATGY | 1 cut(s) | 585 |
| BstSCI | CCNGG | 4 cut(s) | 66, 110, 325, 492 |
| BstSFI | CTRYAG | 1 cut(s) | 415 |
| BstSLI | GKGCMC | 1 cut(s) | 399 |
| BstUI | CGCG | 2 cut(s) | 792, 815 |
| BstV1I | GCAGC | 3 cut(s) | 429, 521, 605 |
| BstV2I | GAAGAC | 1 cut(s) | 824 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1035 |
| BsuRI | GGCC | 2 cut(s) | 945, 1033 |
| BtgZI | GCGATG | 1 cut(s) | 971 |
| BtsCI | GGATG | 1 cut(s) | 366 |
| BtsIMutI | CAGTG | 2 cut(s) | 378, 386 |
| Cac8I | GCNNGC | 3 cut(s) | 406, 583, 587 |
| CciI | TCATGA | 1 cut(s) | 846 |
| Cfr13I | GGNCC | 2 cut(s) | 490, 944 |
| CseI | GACGC | 1 cut(s) | 781 |
| Csp6I | GTAC | 3 cut(s) | 71, 504, 697 |
| CviQI | GTAC | 3 cut(s) | 71, 504, 697 |
| DdeI | CTNAG | 2 cut(s) | 915, 1035 |
| DpnI | GATC | 2 cut(s) | 148, 997 |
| DpnII | GATC | 2 cut(s) | 146, 995 |
| Eam1104I | CTCTTC | 3 cut(s) | 71, 255, 261 |
| EarI | CTCTTC | 3 cut(s) | 71, 255, 261 |
| Eco32I | GATATC | 1 cut(s) | 83 |
| Eco47I | GGWCC | 1 cut(s) | 490 |
| Eco57I | CTGAAG | 1 cut(s) | 95 |
| Eco81I | CCTNAGG | 1 cut(s) | 1035 |
| EcoO109I | RGGNCCY | 1 cut(s) | 490 |
| EcoRII | CCWGG | 3 cut(s) | 110, 325, 492 |
| EcoRV | GATATC | 1 cut(s) | 83 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 493, 525 |
| FblI | GTMKAC | 2 cut(s) | 16, 962 |
| Fnu4HI | GCNGC | 3 cut(s) | 418, 535, 619 |
| FokI | GGATG | 1 cut(s) | 353 |
| Fsp4HI | GCNGC | 3 cut(s) | 418, 535, 619 |
| FspBI | CTAG | 5 cut(s) | 182, 450, 465, 639, 840 |
| GluI | GCNGC | 3 cut(s) | 418, 535, 619 |
| HaeIII | GGCC | 2 cut(s) | 945, 1033 |
| HapII | CCGG | 1 cut(s) | 68 |
| HgaI | GACGC | 1 cut(s) | 781 |
| HincII | GTYRAC | 1 cut(s) | 17 |
| HindII | GTYRAC | 1 cut(s) | 17 |
| HinfI | GANTC | 3 cut(s) | 497, 787, 854 |
| HpaII | CCGG | 1 cut(s) | 68 |
| HphI | GGTGA | 6 cut(s) | 16, 38, 304, 305, 716, 829 |
| Hpy166II | GTNNAC | 3 cut(s) | 17, 741, 963 |
| Hpy188I | TCNGA | 2 cut(s) | 151, 995 |
| Hpy188III | TCNNGA | 6 cut(s) | 89, 164, 191, 752, 784, 847 |
| Hpy8I | GTNNAC | 3 cut(s) | 17, 741, 963 |
| Hpy99I | CGWCG | 1 cut(s) | 90 |
| HpyAV | CCTTC | 7 cut(s) | 38, 108, 481, 758, 794, 844, 960 |
| HpyCH4III | ACNGT | 3 cut(s) | 704, 745, 906 |
| HpyCH4V | TGCA | 3 cut(s) | 417, 537, 585 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 414, 459, 531, 927 |
| HpyF3I | CTNAG | 2 cut(s) | 915, 1035 |
| Kzo9I | GATC | 2 cut(s) | 146, 995 |
| LguI | GCTCTTC | 1 cut(s) | 261 |
| LmnI | GCTCC | 1 cut(s) | 425 |
| Lsp1109I | GCAGC | 3 cut(s) | 429, 521, 605 |
| MaeI | CTAG | 5 cut(s) | 182, 450, 465, 639, 840 |
| MaeIII | GTNAC | 1 cut(s) | 310 |
| MalI | GATC | 2 cut(s) | 148, 997 |
| MboI | GATC | 2 cut(s) | 146, 995 |
| MhlI | GDGCHC | 1 cut(s) | 399 |
| MluCI | AATT | 5 cut(s) | 212, 274, 345, 689, 826 |
| MlyI | GAGTC | 1 cut(s) | 863 |
| MmeI | TCCRAC | 1 cut(s) | 31 |
| MseI | TTAA | 6 cut(s) | 306, 317, 362, 825, 876, 974 |
| MslI | CAYNNNNRTG | 1 cut(s) | 371 |
| MspI | CCGG | 1 cut(s) | 68 |
| MspR9I | CCNGG | 4 cut(s) | 68, 112, 327, 494 |
| MvaI | CCWGG | 3 cut(s) | 112, 327, 494 |
| MvnI | CGCG | 2 cut(s) | 792, 815 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 414, 459, 531, 927 |
| NciI | CCSGG | 1 cut(s) | 68 |
| NdeII | GATC | 2 cut(s) | 146, 995 |
| NlaIV | GGNNCC | 1 cut(s) | 58 |
| NmuCI | GTSAC | 1 cut(s) | 310 |
| NspI | RCATGY | 1 cut(s) | 585 |
| PaeI | GCATGC | 1 cut(s) | 585 |
| PagI | TCATGA | 1 cut(s) | 846 |
| PciSI | GCTCTTC | 1 cut(s) | 261 |
| PfeI | GAWTC | 2 cut(s) | 497, 787 |
| PflFI | GACNNNGTC | 2 cut(s) | 12, 626 |
| PfoI | TCCNGGA | 1 cut(s) | 492 |
| PkrI | GCNGC | 3 cut(s) | 419, 536, 620 |
| PleI | GAGTC | 1 cut(s) | 862 |
| PpsI | GAGTC | 1 cut(s) | 862 |
| PpuMI | RGGWCCY | 1 cut(s) | 490 |
| PshBI | ATTAAT | 1 cut(s) | 306 |
| Psp5II | RGGWCCY | 1 cut(s) | 490 |
| Psp6I | CCWGG | 3 cut(s) | 110, 325, 492 |
| PspGI | CCWGG | 3 cut(s) | 110, 325, 492 |
| PspN4I | GGNNCC | 1 cut(s) | 58 |
| PspPI | GGNCC | 2 cut(s) | 490, 944 |
| PspPPI | RGGWCCY | 1 cut(s) | 490 |
| PstI | CTGCAG | 1 cut(s) | 419 |
| PsyI | GACNNNGTC | 2 cut(s) | 12, 626 |
| RsaI | GTAC | 3 cut(s) | 72, 505, 698 |
| RsaNI | GTAC | 3 cut(s) | 71, 504, 697 |
| RseI | CAYNNNNRTG | 1 cut(s) | 371 |
| SalI | GTCGAC | 1 cut(s) | 15 |
| SapI | GCTCTTC | 1 cut(s) | 261 |
| SaqAI | TTAA | 6 cut(s) | 306, 317, 362, 825, 876, 974 |
| SatI | GCNGC | 3 cut(s) | 418, 535, 619 |
| Sau3AI | GATC | 2 cut(s) | 146, 995 |
| Sau96I | GGNCC | 2 cut(s) | 490, 944 |
| SchI | GAGTC | 1 cut(s) | 863 |
| ScrFI | CCNGG | 4 cut(s) | 68, 112, 327, 494 |
| SduI | GDGCHC | 1 cut(s) | 399 |
| SfcI | CTRYAG | 1 cut(s) | 415 |
| SinI | GGWCC | 1 cut(s) | 490 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 371 |
| SmlI | CTYRAG | 1 cut(s) | 782 |
| SmoI | CTYRAG | 1 cut(s) | 782 |
| SphI | GCATGC | 1 cut(s) | 585 |
| Sse9I | AATT | 5 cut(s) | 212, 274, 345, 689, 826 |
| SsiI | CCGC | 1 cut(s) | 815 |
| SspI | AATATT | 1 cut(s) | 892 |
| SspMI | CTAG | 5 cut(s) | 182, 450, 465, 639, 840 |
| StyD4I | CCNGG | 4 cut(s) | 66, 110, 325, 492 |
| TaaI | ACNGT | 3 cut(s) | 704, 745, 906 |
| TaqI | TCGA | 6 cut(s) | 16, 88, 252, 517, 798, 984 |
| TasI | AATT | 5 cut(s) | 212, 274, 345, 689, 826 |
| TatI | WGTACW | 1 cut(s) | 503 |
| TfiI | GAWTC | 2 cut(s) | 497, 787 |
| Tru1I | TTAA | 6 cut(s) | 306, 317, 362, 825, 876, 974 |
| Tru9I | TTAA | 6 cut(s) | 306, 317, 362, 825, 876, 974 |
| TscAI | CASTG | 2 cut(s) | 378, 386 |
| TseFI | GTSAC | 1 cut(s) | 310 |
| TseI | GCWGC | 3 cut(s) | 417, 534, 618 |
| Tsp45I | GTSAC | 1 cut(s) | 310 |
| TspGWI | ACGGA | 1 cut(s) | 900 |
| TspRI | CASTG | 2 cut(s) | 378, 386 |
| Tth111I | GACNNNGTC | 2 cut(s) | 12, 626 |
| VpaK11BI | GGWCC | 1 cut(s) | 490 |
| VspI | ATTAAT | 1 cut(s) | 306 |
| XapI | RAATTY | 2 cut(s) | 212, 689 |
| XceI | RCATGY | 1 cut(s) | 585 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 938 |
| XmiI | GTMKAC | 2 cut(s) | 16, 962 |
| XspI | CTAG | 5 cut(s) | 182, 450, 465, 639, 840 |