E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 735 |
| AarI | CACCTGC | 1 cut(s) | 722 |
| AatII | GACGTC | 1 cut(s) | 603 |
| Acc36I | ACCTGC | 1 cut(s) | 722 |
| AccI | GTMKAC | 1 cut(s) | 645 |
| AccII | CGCG | 1 cut(s) | 379 |
| AclI | AACGTT | 1 cut(s) | 759 |
| AclWI | GGATC | 3 cut(s) | 42, 55, 1293 |
| AcoI | YGGCCR | 1 cut(s) | 1204 |
| AcsI | RAATTY | 1 cut(s) | 91 |
| AcuI | CTGAAG | 2 cut(s) | 913, 1163 |
| AcyI | GRCGYC | 2 cut(s) | 291, 600 |
| AfaI | GTAC | 4 cut(s) | 144, 410, 920, 968 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 720, 884, 1282 |
| AgsI | TTSAA | 2 cut(s) | 1022, 1103 |
| AjnI | CCWGG | 5 cut(s) | 59, 277, 719, 1201, 1206 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1056, 1088 |
| AluBI | AGCT | 4 cut(s) | 181, 338, 671, 1110 |
| AluI | AGCT | 4 cut(s) | 181, 338, 671, 1110 |
| Alw26I | GTCTC | 4 cut(s) | 297, 414, 904, 1175 |
| AlwI | GGATC | 3 cut(s) | 42, 55, 1293 |
| AoxI | GGCC | 4 cut(s) | 71, 722, 1204, 1332 |
| ApeKI | GCWGC | 6 cut(s) | 54, 335, 453, 684, 804, 1272 |
| ApoI | RAATTY | 1 cut(s) | 91 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 585, 617 |
| AspS9I | GGNCC | 2 cut(s) | 637, 723 |
| AsuHPI | GGTGA | 3 cut(s) | 354, 574, 855 |
| AsuII | TTCGAA | 1 cut(s) | 1260 |
| AvaII | GGWCC | 1 cut(s) | 637 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 958, 991 |
| BalI | TGGCCA | 1 cut(s) | 1206 |
| BamHI | GGATCC | 1 cut(s) | 47 |
| BanII | GRGCYC | 1 cut(s) | 630 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 911, 943 |
| BbsI | GAAGAC | 3 cut(s) | 264, 294, 571 |
| BbvI | GCAGC | 6 cut(s) | 66, 347, 465, 671, 791, 1284 |
| BccI | CCATC | 6 cut(s) | 356, 512, 569, 992, 1028, 1276 |
| BciT130I | CCWGG | 5 cut(s) | 61, 279, 721, 1203, 1208 |
| BciVI | GTATCC | 1 cut(s) | 223 |
| BcoDI | GTCTC | 4 cut(s) | 297, 414, 904, 1175 |
| BfmI | CTRYAG | 1 cut(s) | 182 |
| BfuAI | ACCTGC | 1 cut(s) | 722 |
| BfuI | GTATCC | 1 cut(s) | 223 |
| Bme1390I | CCNGG | 5 cut(s) | 61, 279, 721, 1203, 1208 |
| Bme18I | GGWCC | 1 cut(s) | 637 |
| BmgT120I | GGNCC | 2 cut(s) | 637, 723 |
| BmiI | GGNNCC | 3 cut(s) | 49, 495, 639 |
| BmrFI | CCNGG | 5 cut(s) | 61, 279, 721, 1203, 1208 |
| BmrI | ACTGGG | 2 cut(s) | 400, 874 |
| BmsI | GCATC | 4 cut(s) | 119, 470, 556, 878 |
| BmuI | ACTGGG | 2 cut(s) | 400, 874 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1180 |
| BpiI | GAAGAC | 3 cut(s) | 264, 294, 571 |
| Bpu14I | TTCGAA | 1 cut(s) | 1260 |
| BpuEI | CTTGAG | 1 cut(s) | 1262 |
| Bsa29I | ATCGAT | 1 cut(s) | 564 |
| BsaHI | GRCGYC | 2 cut(s) | 291, 600 |
| BsaJI | CCNNGG | 2 cut(s) | 59, 377 |
| BsaWI | WCCGGW | 2 cut(s) | 299, 963 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 720, 884, 1282 |
| Bse1I | ACTGG | 3 cut(s) | 406, 649, 880 |
| BseBI | CCWGG | 5 cut(s) | 61, 279, 721, 1203, 1208 |
| BseCI | ATCGAT | 1 cut(s) | 564 |
| BseDI | CCNNGG | 2 cut(s) | 59, 377 |
| BseGI | GGATG | 3 cut(s) | 110, 461, 893 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 720, 884, 1282 |
| BseMII | CTCAG | 1 cut(s) | 1186 |
| BseNI | ACTGG | 3 cut(s) | 406, 649, 880 |
| BseRI | GAGGAG | 2 cut(s) | 486, 667 |
| BseXI | GCAGC | 6 cut(s) | 66, 347, 465, 671, 791, 1284 |
| BseYI | CCCAGC | 1 cut(s) | 1106 |
| BsgI | GTGCAG | 1 cut(s) | 670 |
| Bsh1236I | CGCG | 1 cut(s) | 379 |
| Bsh1285I | CGRYCG | 1 cut(s) | 430 |
| BshFI | GGCC | 4 cut(s) | 73, 724, 1206, 1334 |
| BshVI | ATCGAT | 1 cut(s) | 564 |
| BsiEI | CGRYCG | 1 cut(s) | 430 |
| BsiSI | CCGG | 3 cut(s) | 300, 480, 964 |
| BslFI | GGGAC | 2 cut(s) | 623, 693 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 720, 884, 1282 |
| BsmAI | GTCTC | 4 cut(s) | 297, 414, 904, 1175 |
| BsmFI | GGGAC | 2 cut(s) | 623, 693 |
| BsmI | GAATGC | 2 cut(s) | 372, 1197 |
| BsnI | GGCC | 4 cut(s) | 73, 724, 1206, 1334 |
| Bsp119I | TTCGAA | 1 cut(s) | 1260 |
| Bsp1286I | GDGCHC | 1 cut(s) | 630 |
| Bsp143I | GATC | 5 cut(s) | 47, 186, 1132, 1231, 1285 |
| BspANI | GGCC | 4 cut(s) | 73, 724, 1206, 1334 |
| BspCNI | CTCAG | 1 cut(s) | 1185 |
| BspDI | ATCGAT | 1 cut(s) | 564 |
| BspFNI | CGCG | 1 cut(s) | 379 |
| BspLI | GGNNCC | 3 cut(s) | 49, 495, 639 |
| BspMI | ACCTGC | 1 cut(s) | 722 |
| BspPI | GGATC | 3 cut(s) | 42, 55, 1293 |
| BspT104I | TTCGAA | 1 cut(s) | 1260 |
| BsrI | ACTGG | 3 cut(s) | 406, 649, 880 |
| BssECI | CCNNGG | 2 cut(s) | 59, 377 |
| BssMI | GATC | 5 cut(s) | 47, 186, 1132, 1231, 1285 |
| BssNI | GRCGYC | 2 cut(s) | 291, 600 |
| Bst2UI | CCWGG | 5 cut(s) | 61, 279, 721, 1203, 1208 |
| Bst4CI | ACNGT | 4 cut(s) | 86, 431, 637, 974 |
| Bst6I | CTCTTC | 2 cut(s) | 318, 1023 |
| BstACI | GRCGYC | 2 cut(s) | 291, 600 |
| BstBI | TTCGAA | 1 cut(s) | 1260 |
| BstC8I | GCNNGC | 3 cut(s) | 124, 673, 911 |
| BstDEI | CTNAG | 1 cut(s) | 1172 |
| BstDSI | CCRYGG | 1 cut(s) | 377 |
| BstF5I | GGATG | 3 cut(s) | 110, 461, 893 |
| BstFNI | CGCG | 1 cut(s) | 379 |
| BstKTI | GATC | 5 cut(s) | 50, 189, 1135, 1234, 1288 |
| BstMAI | GTCTC | 4 cut(s) | 297, 414, 904, 1175 |
| BstMBI | GATC | 5 cut(s) | 47, 186, 1132, 1231, 1285 |
| BstMCI | CGRYCG | 1 cut(s) | 430 |
| BstNI | CCWGG | 5 cut(s) | 61, 279, 721, 1203, 1208 |
| BstNSI | RCATGY | 1 cut(s) | 855 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1180 |
| BstSCI | CCNGG | 5 cut(s) | 59, 277, 719, 1201, 1206 |
| BstSFI | CTRYAG | 1 cut(s) | 182 |
| BstUI | CGCG | 1 cut(s) | 379 |
| BstV1I | GCAGC | 6 cut(s) | 66, 347, 465, 671, 791, 1284 |
| BstV2I | GAAGAC | 3 cut(s) | 264, 294, 571 |
| BstX2I | RGATCY | 2 cut(s) | 47, 1285 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1114 |
| BstYI | RGATCY | 2 cut(s) | 47, 1285 |
| Bsu15I | ATCGAT | 1 cut(s) | 564 |
| BsuI | GTATCC | 1 cut(s) | 223 |
| BsuRI | GGCC | 4 cut(s) | 73, 724, 1206, 1334 |
| BsuTUI | ATCGAT | 1 cut(s) | 564 |
| BtgI | CCRYGG | 1 cut(s) | 377 |
| BtsCI | GGATG | 3 cut(s) | 110, 461, 893 |
| BtsIMutI | CAGTG | 1 cut(s) | 656 |
| BveI | ACCTGC | 1 cut(s) | 722 |
| Cac8I | GCNNGC | 3 cut(s) | 124, 673, 911 |
| Cfr13I | GGNCC | 2 cut(s) | 637, 723 |
| Cfr42I | CCGCGG | 1 cut(s) | 380 |
| ClaI | ATCGAT | 1 cut(s) | 564 |
| CseI | GACGC | 1 cut(s) | 299 |
| Csp6I | GTAC | 4 cut(s) | 143, 409, 919, 967 |
| CviAII | CATG | 8 cut(s) | 783, 800, 842, 852, 873, 941, 1114, 1248 |
| CviQI | GTAC | 4 cut(s) | 143, 409, 919, 967 |
| DdeI | CTNAG | 1 cut(s) | 1172 |
| DpnI | GATC | 5 cut(s) | 49, 188, 1134, 1233, 1287 |
| DpnII | GATC | 5 cut(s) | 47, 186, 1132, 1231, 1285 |
| EaeI | YGGCCR | 1 cut(s) | 1204 |
| Eam1104I | CTCTTC | 2 cut(s) | 318, 1023 |
| EarI | CTCTTC | 2 cut(s) | 318, 1023 |
| EciI | GGCGGA | 2 cut(s) | 36, 109 |
| Eco147I | AGGCCT | 1 cut(s) | 73 |
| Eco24I | GRGCYC | 1 cut(s) | 630 |
| Eco47I | GGWCC | 1 cut(s) | 637 |
| Eco57I | CTGAAG | 2 cut(s) | 913, 1163 |
| EcoRI | GAATTC | 1 cut(s) | 91 |
| EcoRII | CCWGG | 5 cut(s) | 59, 277, 719, 1201, 1206 |
| EcoT38I | GRGCYC | 1 cut(s) | 630 |
| FaeI | CATG | 8 cut(s) | 786, 803, 845, 855, 876, 944, 1117, 1251 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1056, 1088 |
| FaqI | GGGAC | 2 cut(s) | 623, 693 |
| FatI | CATG | 8 cut(s) | 782, 799, 841, 851, 872, 940, 1113, 1247 |
| FauI | CCCGC | 6 cut(s) | 59, 520, 526, 535, 541, 547 |
| FauNDI | CATATG | 1 cut(s) | 1295 |
| FblI | GTMKAC | 1 cut(s) | 645 |
| FokI | GGATG | 3 cut(s) | 97, 448, 900 |
| FriOI | GRGCYC | 1 cut(s) | 630 |
| GsaI | CCCAGC | 1 cut(s) | 1110 |
| HaeIII | GGCC | 4 cut(s) | 73, 724, 1206, 1334 |
| HapII | CCGG | 3 cut(s) | 300, 480, 964 |
| HgaI | GACGC | 1 cut(s) | 299 |
| Hin1I | GRCGYC | 2 cut(s) | 291, 600 |
| Hin1II | CATG | 8 cut(s) | 786, 803, 845, 855, 876, 944, 1117, 1251 |
| HinfI | GANTC | 5 cut(s) | 9, 138, 483, 593, 812 |
| HpaII | CCGG | 3 cut(s) | 300, 480, 964 |
| HphI | GGTGA | 3 cut(s) | 354, 574, 855 |
| Hpy166II | GTNNAC | 2 cut(s) | 646, 946 |
| Hpy188I | TCNGA | 4 cut(s) | 81, 90, 598, 1175 |
| Hpy188III | TCNNGA | 4 cut(s) | 764, 885, 1022, 1279 |
| Hpy8I | GTNNAC | 2 cut(s) | 646, 946 |
| Hpy99I | CGWCG | 5 cut(s) | 102, 194, 266, 269, 314 |
| HpyAV | CCTTC | 3 cut(s) | 32, 510, 1230 |
| HpyCH4III | ACNGT | 4 cut(s) | 86, 431, 637, 974 |
| HpyCH4IV | ACGT | 4 cut(s) | 600, 759, 989, 1059 |
| HpyCH4V | TGCA | 5 cut(s) | 461, 687, 807, 891, 953 |
| HpyF3I | CTNAG | 1 cut(s) | 1172 |
| HpySE526I | ACGT | 4 cut(s) | 600, 759, 989, 1059 |
| Hsp92I | GRCGYC | 2 cut(s) | 291, 600 |
| Hsp92II | CATG | 8 cut(s) | 786, 803, 845, 855, 876, 944, 1117, 1251 |
| KspI | CCGCGG | 1 cut(s) | 380 |
| Kzo9I | GATC | 5 cut(s) | 47, 186, 1132, 1231, 1285 |
| LmnI | GCTCC | 3 cut(s) | 499, 680, 722 |
| Lsp1109I | GCAGC | 6 cut(s) | 66, 347, 465, 671, 791, 1284 |
| LweI | GCATC | 4 cut(s) | 119, 470, 556, 878 |
| MaeII | ACGT | 4 cut(s) | 600, 759, 989, 1059 |
| MalI | GATC | 5 cut(s) | 49, 188, 1134, 1233, 1287 |
| MboI | GATC | 5 cut(s) | 47, 186, 1132, 1231, 1285 |
| MflI | RGATCY | 2 cut(s) | 47, 1285 |
| MhlI | GDGCHC | 1 cut(s) | 630 |
| MlsI | TGGCCA | 1 cut(s) | 1206 |
| MluCI | AATT | 2 cut(s) | 91, 342 |
| MluNI | TGGCCA | 1 cut(s) | 1206 |
| MlyI | GAGTC | 2 cut(s) | 492, 821 |
| Mox20I | TGGCCA | 1 cut(s) | 1206 |
| MscI | TGGCCA | 1 cut(s) | 1206 |
| MseI | TTAA | 1 cut(s) | 105 |
| Msp20I | TGGCCA | 1 cut(s) | 1206 |
| MspA1I | CMGCKG | 2 cut(s) | 338, 379 |
| MspI | CCGG | 3 cut(s) | 300, 480, 964 |
| MspR9I | CCNGG | 5 cut(s) | 61, 279, 721, 1203, 1208 |
| Mva1269I | GAATGC | 2 cut(s) | 372, 1197 |
| MvaI | CCWGG | 5 cut(s) | 61, 279, 721, 1203, 1208 |
| MvnI | CGCG | 1 cut(s) | 379 |
| NdeI | CATATG | 1 cut(s) | 1295 |
| NdeII | GATC | 5 cut(s) | 47, 186, 1132, 1231, 1285 |
| NlaIII | CATG | 8 cut(s) | 786, 803, 845, 855, 876, 944, 1117, 1251 |
| NlaIV | GGNNCC | 3 cut(s) | 49, 495, 639 |
| NmeAIII | GCCGAG | 1 cut(s) | 770 |
| NspI | RCATGY | 1 cut(s) | 855 |
| NspV | TTCGAA | 1 cut(s) | 1260 |
| PaqCI | CACCTGC | 1 cut(s) | 722 |
| PceI | AGGCCT | 1 cut(s) | 73 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 39 |
| PctI | GAATGC | 2 cut(s) | 372, 1197 |
| PfeI | GAWTC | 3 cut(s) | 9, 138, 593 |
| PfoI | TCCNGGA | 1 cut(s) | 277 |
| PleI | GAGTC | 2 cut(s) | 491, 820 |
| PpsI | GAGTC | 2 cut(s) | 491, 820 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1180 |
| PsiI | TTATAA | 1 cut(s) | 735 |
| Psp1406I | AACGTT | 1 cut(s) | 759 |
| Psp6I | CCWGG | 5 cut(s) | 59, 277, 719, 1201, 1206 |
| PspFI | CCCAGC | 1 cut(s) | 1106 |
| PspGI | CCWGG | 5 cut(s) | 59, 277, 719, 1201, 1206 |
| PspN4I | GGNNCC | 3 cut(s) | 49, 495, 639 |
| PspPI | GGNCC | 2 cut(s) | 637, 723 |
| PsuI | RGATCY | 2 cut(s) | 47, 1285 |
| PvuII | CAGCTG | 1 cut(s) | 338 |
| RsaI | GTAC | 4 cut(s) | 144, 410, 920, 968 |
| RsaNI | GTAC | 4 cut(s) | 143, 409, 919, 967 |
| SacII | CCGCGG | 1 cut(s) | 380 |
| SaqAI | TTAA | 1 cut(s) | 105 |
| Sau3AI | GATC | 5 cut(s) | 47, 186, 1132, 1231, 1285 |
| Sau96I | GGNCC | 2 cut(s) | 637, 723 |
| SchI | GAGTC | 2 cut(s) | 492, 821 |
| ScrFI | CCNGG | 5 cut(s) | 61, 279, 721, 1203, 1208 |
| SduI | GDGCHC | 1 cut(s) | 630 |
| SfaNI | GCATC | 4 cut(s) | 119, 470, 556, 878 |
| SfcI | CTRYAG | 1 cut(s) | 182 |
| Sfr303I | CCGCGG | 1 cut(s) | 380 |
| SfuI | TTCGAA | 1 cut(s) | 1260 |
| SgrBI | CCGCGG | 1 cut(s) | 380 |
| SinI | GGWCC | 1 cut(s) | 637 |
| SmlI | CTYRAG | 1 cut(s) | 1277 |
| SmoI | CTYRAG | 1 cut(s) | 1277 |
| Sse9I | AATT | 2 cut(s) | 91, 342 |
| SseBI | AGGCCT | 1 cut(s) | 73 |
| StuI | AGGCCT | 1 cut(s) | 73 |
| StyD4I | CCNGG | 5 cut(s) | 59, 277, 719, 1201, 1206 |
| TaaI | ACNGT | 4 cut(s) | 86, 431, 637, 974 |
| TaiI | ACGT | 4 cut(s) | 603, 762, 992, 1062 |
| TaqI | TCGA | 9 cut(s) | 100, 192, 564, 815, 1053, 1088, 1144, 1226, 1260 |
| TasI | AATT | 2 cut(s) | 91, 342 |
| TauI | GCSGC | 7 cut(s) | 154, 157, 160, 382, 385, 479, 524 |
| TfiI | GAWTC | 3 cut(s) | 9, 138, 593 |
| Tru1I | TTAA | 1 cut(s) | 105 |
| Tru9I | TTAA | 1 cut(s) | 105 |
| TscAI | CASTG | 1 cut(s) | 656 |
| TseI | GCWGC | 6 cut(s) | 54, 335, 453, 684, 804, 1272 |
| TspDTI | ATGAA | 9 cut(s) | 219, 300, 336, 604, 771, 852, 858, 861, 1102 |
| TspGWI | ACGGA | 1 cut(s) | 48 |
| TspRI | CASTG | 1 cut(s) | 656 |
| VpaK11BI | GGWCC | 1 cut(s) | 637 |
| XapI | RAATTY | 1 cut(s) | 91 |
| XceI | RCATGY | 1 cut(s) | 855 |
| XmiI | GTMKAC | 1 cut(s) | 645 |
| ZraI | GACGTC | 1 cut(s) | 601 |