E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 671 |
| AccII | CGCG | 1 cut(s) | 527 |
| AciI | CCGC | 1 cut(s) | 259 |
| AclWI | GGATC | 1 cut(s) | 24 |
| AcsI | RAATTY | 2 cut(s) | 677, 879 |
| AdeI | CACNNNGTG | 1 cut(s) | 659 |
| AfaI | GTAC | 4 cut(s) | 236, 437, 449, 518 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 387, 388, 671 |
| AflIII | ACRYGT | 2 cut(s) | 525, 535 |
| AgeI | ACCGGT | 1 cut(s) | 542 |
| AgsI | TTSAA | 6 cut(s) | 505, 572, 605, 728, 752, 871 |
| AjiI | CACGTC | 1 cut(s) | 538 |
| AjnI | CCWGG | 1 cut(s) | 664 |
| AleI | CACNNNNGTG | 1 cut(s) | 443 |
| AluBI | AGCT | 2 cut(s) | 130, 558 |
| AluI | AGCT | 2 cut(s) | 130, 558 |
| Alw21I | GWGCWC | 1 cut(s) | 664 |
| Alw26I | GTCTC | 3 cut(s) | 67, 319, 624 |
| AlwI | GGATC | 1 cut(s) | 24 |
| ApeKI | GCWGC | 1 cut(s) | 339 |
| ApoI | RAATTY | 2 cut(s) | 677, 879 |
| AsiGI | ACCGGT | 1 cut(s) | 542 |
| AspS9I | GGNCC | 1 cut(s) | 587 |
| AsuHPI | GGTGA | 4 cut(s) | 241, 532, 557, 824 |
| AvaII | GGWCC | 1 cut(s) | 587 |
| BanII | GRGCYC | 2 cut(s) | 175, 764 |
| Bbv12I | GWGCWC | 1 cut(s) | 664 |
| BbvI | GCAGC | 1 cut(s) | 326 |
| BccI | CCATC | 4 cut(s) | 158, 578, 607, 696 |
| BceAI | ACGGC | 1 cut(s) | 102 |
| BciT130I | CCWGG | 1 cut(s) | 666 |
| BclI | TGATCA | 1 cut(s) | 490 |
| BcoDI | GTCTC | 3 cut(s) | 67, 319, 624 |
| BfaI | CTAG | 1 cut(s) | 279 |
| BfmI | CTRYAG | 1 cut(s) | 250 |
| BglII | AGATCT | 1 cut(s) | 454 |
| BisI | GCNGC | 1 cut(s) | 340 |
| BlsI | GCNGC | 1 cut(s) | 341 |
| Bme1390I | CCNGG | 1 cut(s) | 666 |
| Bme18I | GGWCC | 1 cut(s) | 587 |
| BmgBI | CACGTC | 1 cut(s) | 538 |
| BmgT120I | GGNCC | 1 cut(s) | 587 |
| BmiI | GGNNCC | 1 cut(s) | 174 |
| BmrFI | CCNGG | 1 cut(s) | 666 |
| BmsI | GCATC | 1 cut(s) | 864 |
| BpmI | CTGGAG | 1 cut(s) | 648 |
| BsaAI | YACGTR | 1 cut(s) | 809 |
| BsaJI | CCNNGG | 1 cut(s) | 66 |
| BsaWI | WCCGGW | 2 cut(s) | 542, 699 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 891 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 387, 388, 671 |
| Bse118I | RCCGGY | 2 cut(s) | 440, 542 |
| Bse1I | ACTGG | 1 cut(s) | 291 |
| BseBI | CCWGG | 1 cut(s) | 666 |
| BseDI | CCNNGG | 1 cut(s) | 66 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 387, 388, 671 |
| BseNI | ACTGG | 1 cut(s) | 291 |
| BseXI | GCAGC | 1 cut(s) | 326 |
| BsgI | GTGCAG | 1 cut(s) | 325 |
| Bsh1236I | CGCG | 1 cut(s) | 527 |
| Bsh1285I | CGRYCG | 1 cut(s) | 40 |
| BshTI | ACCGGT | 1 cut(s) | 542 |
| BsiEI | CGRYCG | 1 cut(s) | 40 |
| BsiHKAI | GWGCWC | 1 cut(s) | 664 |
| BsiSI | CCGG | 3 cut(s) | 441, 543, 700 |
| BslFI | GGGAC | 1 cut(s) | 132 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 387, 388, 671 |
| BsmAI | GTCTC | 3 cut(s) | 67, 319, 624 |
| BsmFI | GGGAC | 1 cut(s) | 132 |
| BsmI | GAATGC | 1 cut(s) | 885 |
| Bsp1286I | GDGCHC | 3 cut(s) | 175, 664, 764 |
| Bsp1407I | TGTACA | 1 cut(s) | 447 |
| Bsp143I | GATC | 7 cut(s) | 29, 110, 133, 275, 454, 490, 893 |
| Bsp19I | CCATGG | 1 cut(s) | 66 |
| BspACI | CCGC | 1 cut(s) | 259 |
| BspFNI | CGCG | 1 cut(s) | 527 |
| BspLI | GGNNCC | 1 cut(s) | 174 |
| BspPI | GGATC | 1 cut(s) | 24 |
| BsrFI | RCCGGY | 2 cut(s) | 440, 542 |
| BsrGI | TGTACA | 1 cut(s) | 447 |
| BsrI | ACTGG | 1 cut(s) | 291 |
| BssAI | RCCGGY | 2 cut(s) | 440, 542 |
| BssECI | CCNNGG | 1 cut(s) | 66 |
| BssMI | GATC | 7 cut(s) | 29, 110, 133, 275, 454, 490, 893 |
| BssT1I | CCWWGG | 1 cut(s) | 66 |
| Bst2UI | CCWGG | 1 cut(s) | 666 |
| Bst4CI | ACNGT | 7 cut(s) | 41, 123, 146, 251, 464, 640, 647 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 659 |
| BstAUI | TGTACA | 1 cut(s) | 447 |
| BstBAI | YACGTR | 1 cut(s) | 809 |
| BstDSI | CCRYGG | 1 cut(s) | 66 |
| BstFNI | CGCG | 1 cut(s) | 527 |
| BstKTI | GATC | 7 cut(s) | 32, 113, 136, 278, 457, 493, 896 |
| BstMAI | GTCTC | 3 cut(s) | 67, 319, 624 |
| BstMBI | GATC | 7 cut(s) | 29, 110, 133, 275, 454, 490, 893 |
| BstMCI | CGRYCG | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 113, 659 |
| BstNI | CCWGG | 1 cut(s) | 666 |
| BstNSI | RCATGY | 1 cut(s) | 210 |
| BstSCI | CCNGG | 1 cut(s) | 664 |
| BstSFI | CTRYAG | 1 cut(s) | 250 |
| BstUI | CGCG | 1 cut(s) | 527 |
| BstV1I | GCAGC | 1 cut(s) | 326 |
| BstX2I | RGATCY | 1 cut(s) | 454 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 167 |
| BstYI | RGATCY | 1 cut(s) | 454 |
| BtgI | CCRYGG | 1 cut(s) | 66 |
| BtrI | CACGTC | 1 cut(s) | 538 |
| BtsIMutI | CAGTG | 2 cut(s) | 119, 191 |
| Cfr10I | RCCGGY | 2 cut(s) | 440, 542 |
| Cfr13I | GGNCC | 1 cut(s) | 587 |
| Csp6I | GTAC | 4 cut(s) | 235, 436, 448, 517 |
| CspAI | ACCGGT | 1 cut(s) | 542 |
| CviAII | CATG | 9 cut(s) | 67, 207, 318, 383, 473, 498, 623, 773, 829 |
| CviJI | RGCY | 9 cut(s) | 130, 173, 339, 380, 558, 762, 790, 818, 860 |
| CviKI_1 | RGCY | 9 cut(s) | 130, 173, 339, 380, 558, 762, 790, 818, 860 |
| CviQI | GTAC | 4 cut(s) | 235, 436, 448, 517 |
| DpnI | GATC | 7 cut(s) | 31, 112, 135, 277, 456, 492, 895 |
| DpnII | GATC | 7 cut(s) | 29, 110, 133, 275, 454, 490, 893 |
| DraIII | CACNNNGTG | 1 cut(s) | 659 |
| Eco130I | CCWWGG | 1 cut(s) | 66 |
| Eco24I | GRGCYC | 2 cut(s) | 175, 764 |
| Eco47I | GGWCC | 1 cut(s) | 587 |
| EcoRI | GAATTC | 1 cut(s) | 677 |
| EcoRII | CCWGG | 1 cut(s) | 664 |
| EcoT14I | CCWWGG | 1 cut(s) | 66 |
| EcoT38I | GRGCYC | 2 cut(s) | 175, 764 |
| ErhI | CCWWGG | 1 cut(s) | 66 |
| FaeI | CATG | 9 cut(s) | 70, 210, 321, 386, 476, 501, 626, 776, 832 |
| FaqI | GGGAC | 1 cut(s) | 132 |
| FatI | CATG | 9 cut(s) | 66, 206, 317, 382, 472, 497, 622, 772, 828 |
| FauNDI | CATATG | 1 cut(s) | 358 |
| FbaI | TGATCA | 1 cut(s) | 490 |
| Fnu4HI | GCNGC | 1 cut(s) | 340 |
| FriOI | GRGCYC | 2 cut(s) | 175, 764 |
| Fsp4HI | GCNGC | 1 cut(s) | 340 |
| FspBI | CTAG | 1 cut(s) | 279 |
| GluI | GCNGC | 1 cut(s) | 340 |
| GsuI | CTGGAG | 1 cut(s) | 648 |
| HapII | CCGG | 3 cut(s) | 441, 543, 700 |
| Hin1II | CATG | 9 cut(s) | 70, 210, 321, 386, 476, 501, 626, 776, 832 |
| HindIII | AAGCTT | 1 cut(s) | 556 |
| HinfI | GANTC | 8 cut(s) | 352, 368, 374, 617, 728, 748, 793, 835 |
| HpaII | CCGG | 3 cut(s) | 441, 543, 700 |
| HphI | GGTGA | 4 cut(s) | 241, 532, 557, 824 |
| Hpy166II | GTNNAC | 5 cut(s) | 402, 438, 448, 450, 643 |
| Hpy188I | TCNGA | 2 cut(s) | 25, 373 |
| Hpy188III | TCNNGA | 5 cut(s) | 13, 140, 279, 572, 897 |
| Hpy8I | GTNNAC | 5 cut(s) | 402, 438, 448, 450, 643 |
| HpyAV | CCTTC | 1 cut(s) | 578 |
| HpyCH4III | ACNGT | 7 cut(s) | 41, 123, 146, 251, 464, 640, 647 |
| HpyCH4IV | ACGT | 3 cut(s) | 537, 722, 808 |
| HpyCH4V | TGCA | 4 cut(s) | 342, 432, 877, 885 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 113, 659 |
| HpySE526I | ACGT | 3 cut(s) | 537, 722, 808 |
| Hsp92II | CATG | 9 cut(s) | 70, 210, 321, 386, 476, 501, 626, 776, 832 |
| Ksp22I | TGATCA | 1 cut(s) | 490 |
| Kzo9I | GATC | 7 cut(s) | 29, 110, 133, 275, 454, 490, 893 |
| LmnI | GCTCC | 3 cut(s) | 127, 178, 667 |
| Lsp1109I | GCAGC | 1 cut(s) | 326 |
| LweI | GCATC | 1 cut(s) | 864 |
| MaeI | CTAG | 1 cut(s) | 279 |
| MaeII | ACGT | 3 cut(s) | 537, 722, 808 |
| MaeIII | GTNAC | 4 cut(s) | 188, 531, 538, 634 |
| MalI | GATC | 7 cut(s) | 31, 112, 135, 277, 456, 492, 895 |
| MboI | GATC | 7 cut(s) | 29, 110, 133, 275, 454, 490, 893 |
| MboII | GAAGA | 6 cut(s) | 128, 204, 361, 593, 748, 883 |
| MflI | RGATCY | 1 cut(s) | 454 |
| MhlI | GDGCHC | 3 cut(s) | 175, 664, 764 |
| MluCI | AATT | 6 cut(s) | 294, 551, 677, 781, 823, 879 |
| MluI | ACGCGT | 1 cut(s) | 525 |
| MlyI | GAGTC | 1 cut(s) | 626 |
| MmeI | TCCRAC | 3 cut(s) | 58, 105, 652 |
| MnlI | CCTC | 7 cut(s) | 42, 44, 74, 94, 381, 829, 871 |
| MseI | TTAA | 1 cut(s) | 554 |
| MslI | CAYNNNNRTG | 2 cut(s) | 443, 471 |
| MspI | CCGG | 3 cut(s) | 441, 543, 700 |
| MspR9I | CCNGG | 1 cut(s) | 666 |
| Mva1269I | GAATGC | 1 cut(s) | 885 |
| MvaI | CCWGG | 1 cut(s) | 666 |
| MvnI | CGCG | 1 cut(s) | 527 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 113, 659 |
| NcoI | CCATGG | 1 cut(s) | 66 |
| NdeI | CATATG | 1 cut(s) | 358 |
| NdeII | GATC | 7 cut(s) | 29, 110, 133, 275, 454, 490, 893 |
| NlaIII | CATG | 9 cut(s) | 70, 210, 321, 386, 476, 501, 626, 776, 832 |
| NlaIV | GGNNCC | 1 cut(s) | 174 |
| NmuCI | GTSAC | 3 cut(s) | 188, 538, 634 |
| NspI | RCATGY | 1 cut(s) | 210 |
| OliI | CACNNNNGTG | 1 cut(s) | 443 |
| PctI | GAATGC | 1 cut(s) | 885 |
| PfeI | GAWTC | 7 cut(s) | 352, 368, 374, 728, 748, 793, 835 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 671 |
| PfoI | TCCNGGA | 1 cut(s) | 664 |
| PinAI | ACCGGT | 1 cut(s) | 542 |
| PkrI | GCNGC | 1 cut(s) | 341 |
| PleI | GAGTC | 1 cut(s) | 625 |
| PpsI | GAGTC | 1 cut(s) | 625 |
| Ppu21I | YACGTR | 1 cut(s) | 809 |
| Psp6I | CCWGG | 1 cut(s) | 664 |
| PspGI | CCWGG | 1 cut(s) | 664 |
| PspN4I | GGNNCC | 1 cut(s) | 174 |
| PspPI | GGNCC | 1 cut(s) | 587 |
| PsuI | RGATCY | 1 cut(s) | 454 |
| RsaI | GTAC | 4 cut(s) | 236, 437, 449, 518 |
| RsaNI | GTAC | 4 cut(s) | 235, 436, 448, 517 |
| RseI | CAYNNNNRTG | 2 cut(s) | 443, 471 |
| SaqAI | TTAA | 1 cut(s) | 554 |
| SatI | GCNGC | 1 cut(s) | 340 |
| Sau3AI | GATC | 7 cut(s) | 29, 110, 133, 275, 454, 490, 893 |
| Sau96I | GGNCC | 1 cut(s) | 587 |
| SchI | GAGTC | 1 cut(s) | 626 |
| ScrFI | CCNGG | 1 cut(s) | 666 |
| SduI | GDGCHC | 3 cut(s) | 175, 664, 764 |
| SetI | ASST | 9 cut(s) | 61, 105, 132, 231, 540, 560, 658, 725, 811 |
| SfaNI | GCATC | 1 cut(s) | 864 |
| SfcI | CTRYAG | 1 cut(s) | 250 |
| SgrAI | CRCCGGYG | 2 cut(s) | 440, 542 |
| SinI | GGWCC | 1 cut(s) | 587 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 443, 471 |
| Sse9I | AATT | 6 cut(s) | 294, 551, 677, 781, 823, 879 |
| SsiI | CCGC | 1 cut(s) | 259 |
| SspMI | CTAG | 1 cut(s) | 279 |
| StyD4I | CCNGG | 1 cut(s) | 664 |
| StyI | CCWWGG | 1 cut(s) | 66 |
| TaaI | ACNGT | 7 cut(s) | 41, 123, 146, 251, 464, 640, 647 |
| TaiI | ACGT | 3 cut(s) | 540, 725, 811 |
| TaqI | TCGA | 4 cut(s) | 36, 274, 611, 833 |
| TasI | AATT | 6 cut(s) | 294, 551, 677, 781, 823, 879 |
| TatI | WGTACW | 4 cut(s) | 234, 435, 447, 516 |
| TfiI | GAWTC | 7 cut(s) | 352, 368, 374, 728, 748, 793, 835 |
| Tru1I | TTAA | 1 cut(s) | 554 |
| Tru9I | TTAA | 1 cut(s) | 554 |
| TscAI | CASTG | 2 cut(s) | 126, 191 |
| TseFI | GTSAC | 3 cut(s) | 188, 538, 634 |
| TseI | GCWGC | 1 cut(s) | 339 |
| Tsp45I | GTSAC | 3 cut(s) | 188, 538, 634 |
| TspDTI | ATGAA | 5 cut(s) | 381, 594, 755, 817, 870 |
| TspGWI | ACGGA | 1 cut(s) | 720 |
| TspRI | CASTG | 2 cut(s) | 126, 191 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 671 |
| VpaK11BI | GGWCC | 1 cut(s) | 587 |
| XapI | RAATTY | 2 cut(s) | 677, 879 |
| XbaI | TCTAGA | 1 cut(s) | 278 |
| XceI | RCATGY | 1 cut(s) | 210 |
| XspI | CTAG | 1 cut(s) | 279 |