E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1262 |
| AccB1I | GGYRCC | 2 cut(s) | 69, 193 |
| AccII | CGCG | 1 cut(s) | 366 |
| AciI | CCGC | 3 cut(s) | 61, 1053, 1326 |
| AclWI | GGATC | 2 cut(s) | 808, 1350 |
| AcoI | YGGCCR | 1 cut(s) | 1327 |
| AcsI | RAATTY | 1 cut(s) | 1064 |
| AcuI | CTGAAG | 1 cut(s) | 970 |
| AcyI | GRCGYC | 1 cut(s) | 70 |
| AfaI | GTAC | 3 cut(s) | 54, 917, 1025 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 774, 941, 1286 |
| AflIII | ACRYGT | 1 cut(s) | 908 |
| AgsI | TTSAA | 4 cut(s) | 590, 659, 831, 1163 |
| AjnI | CCWGG | 1 cut(s) | 82 |
| AleI | CACNNNNGTG | 1 cut(s) | 480 |
| AluBI | AGCT | 5 cut(s) | 146, 725, 1155, 1170, 1333 |
| AluI | AGCT | 5 cut(s) | 146, 725, 1155, 1170, 1333 |
| Alw21I | GWGCWC | 2 cut(s) | 148, 1250 |
| AlwI | GGATC | 2 cut(s) | 808, 1350 |
| AoxI | GGCC | 3 cut(s) | 62, 1327, 1355 |
| ApoI | RAATTY | 1 cut(s) | 1064 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 858, 890 |
| AseI | ATTAAT | 1 cut(s) | 750 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 167 |
| AspLEI | GCGC | 1 cut(s) | 72 |
| AspS9I | GGNCC | 3 cut(s) | 63, 135, 777 |
| AsuC2I | CCSGG | 2 cut(s) | 67, 775 |
| AsuHPI | GGTGA | 3 cut(s) | 359, 386, 413 |
| AvaII | GGWCC | 2 cut(s) | 135, 777 |
| AxyI | CCTNAGG | 1 cut(s) | 1359 |
| BaeGI | GKGCMC | 1 cut(s) | 196 |
| BanI | GGYRCC | 2 cut(s) | 69, 193 |
| BanII | GRGCYC | 1 cut(s) | 148 |
| BbsI | GAAGAC | 2 cut(s) | 597, 688 |
| Bbv12I | GWGCWC | 2 cut(s) | 148, 1250 |
| BccI | CCATC | 4 cut(s) | 695, 945, 1333, 1388 |
| BceAI | ACGGC | 2 cut(s) | 783, 1127 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 28 |
| BciT130I | CCWGG | 1 cut(s) | 84 |
| BciVI | GTATCC | 1 cut(s) | 299 |
| BclI | TGATCA | 2 cut(s) | 247, 460 |
| BcnI | CCSGG | 2 cut(s) | 67, 775 |
| BfaI | CTAG | 2 cut(s) | 941, 1133 |
| BfmI | CTRYAG | 1 cut(s) | 1030 |
| BfoI | RGCGCY | 1 cut(s) | 73 |
| BfuI | GTATCC | 1 cut(s) | 299 |
| BglII | AGATCT | 2 cut(s) | 649, 1129 |
| BisI | GCNGC | 2 cut(s) | 62, 1327 |
| BlpI | GCTNAGC | 1 cut(s) | 1151 |
| BlsI | GCNGC | 2 cut(s) | 63, 1328 |
| BmcAI | AGTACT | 1 cut(s) | 54 |
| Bme1390I | CCNGG | 3 cut(s) | 67, 84, 775 |
| Bme18I | GGWCC | 2 cut(s) | 135, 777 |
| BmgT120I | GGNCC | 3 cut(s) | 63, 135, 777 |
| BmiI | GGNNCC | 4 cut(s) | 65, 71, 195, 778 |
| BmrFI | CCNGG | 3 cut(s) | 67, 84, 775 |
| BmsI | GCATC | 5 cut(s) | 48, 132, 290, 747, 851 |
| BpiI | GAAGAC | 2 cut(s) | 597, 688 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1151 |
| BpuEI | CTTGAG | 2 cut(s) | 1094, 1319 |
| BpuMI | CCSGG | 2 cut(s) | 67, 775 |
| Bsa29I | ATCGAT | 1 cut(s) | 535 |
| BsaBI | GATNNNNATC | 7 cut(s) | 360, 387, 414, 444, 477, 507, 537 |
| BsaHI | GRCGYC | 1 cut(s) | 70 |
| BsaJI | CCNNGG | 2 cut(s) | 65, 1209 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 336, 363, 366, 390, 393, 420 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 774, 941, 1286 |
| Bse1I | ACTGG | 1 cut(s) | 1040 |
| Bse21I | CCTNAGG | 1 cut(s) | 1359 |
| Bse3DI | GCAATG | 1 cut(s) | 31 |
| Bse8I | GATNNNNATC | 7 cut(s) | 360, 387, 414, 444, 477, 507, 537 |
| BseBI | CCWGG | 1 cut(s) | 84 |
| BseCI | ATCGAT | 1 cut(s) | 535 |
| BseDI | CCNNGG | 2 cut(s) | 65, 1209 |
| BseGI | GGATG | 7 cut(s) | 126, 281, 687, 866, 962, 1369, 1380 |
| BseJI | GATNNNNATC | 7 cut(s) | 360, 387, 414, 444, 477, 507, 537 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 774, 941, 1286 |
| BseMI | GCAATG | 1 cut(s) | 31 |
| BseMII | CTCAG | 1 cut(s) | 1350 |
| BseNI | ACTGG | 1 cut(s) | 1040 |
| BseRI | GAGGAG | 3 cut(s) | 165, 231, 721 |
| BseSI | GKGCMC | 1 cut(s) | 196 |
| BseYI | CCCAGC | 1 cut(s) | 1166 |
| Bsh1236I | CGCG | 1 cut(s) | 366 |
| BshFI | GGCC | 3 cut(s) | 64, 1329, 1357 |
| BshNI | GGYRCC | 2 cut(s) | 69, 193 |
| BshVI | ATCGAT | 1 cut(s) | 535 |
| BsiHKAI | GWGCWC | 2 cut(s) | 148, 1250 |
| BsiSI | CCGG | 3 cut(s) | 67, 312, 774 |
| BslFI | GGGAC | 2 cut(s) | 747, 918 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 774, 941, 1286 |
| BsmFI | GGGAC | 2 cut(s) | 747, 918 |
| BsmI | GAATGC | 1 cut(s) | 665 |
| BsnI | GGCC | 3 cut(s) | 64, 1329, 1357 |
| Bsp1286I | GDGCHC | 3 cut(s) | 148, 196, 1250 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1151 |
| Bsp68I | TCGCGA | 1 cut(s) | 366 |
| BspACI | CCGC | 3 cut(s) | 61, 1053, 1326 |
| BspANI | GGCC | 3 cut(s) | 64, 1329, 1357 |
| BspCNI | CTCAG | 1 cut(s) | 1351 |
| BspDI | ATCGAT | 1 cut(s) | 535 |
| BspFNI | CGCG | 1 cut(s) | 366 |
| BspHI | TCATGA | 1 cut(s) | 511 |
| BspLI | GGNNCC | 4 cut(s) | 65, 71, 195, 778 |
| BspMAI | CTGCAG | 1 cut(s) | 1034 |
| BspPI | GGATC | 2 cut(s) | 808, 1350 |
| BspT107I | GGYRCC | 2 cut(s) | 69, 193 |
| BsrDI | GCAATG | 1 cut(s) | 31 |
| BsrI | ACTGG | 1 cut(s) | 1040 |
| BssECI | CCNNGG | 2 cut(s) | 65, 1209 |
| BssNI | GRCGYC | 1 cut(s) | 70 |
| BssT1I | CCWWGG | 1 cut(s) | 1209 |
| Bst2UI | CCWGG | 1 cut(s) | 84 |
| Bst6I | CTCTTC | 4 cut(s) | 96, 289, 310, 591 |
| BstACI | GRCGYC | 1 cut(s) | 70 |
| BstC8I | GCNNGC | 2 cut(s) | 727, 1331 |
| BstDEI | CTNAG | 3 cut(s) | 1151, 1223, 1359 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1284 |
| BstF5I | GGATG | 7 cut(s) | 126, 281, 687, 866, 962, 1369, 1380 |
| BstFNI | CGCG | 1 cut(s) | 366 |
| BstH2I | RGCGCY | 1 cut(s) | 73 |
| BstHHI | GCGC | 1 cut(s) | 72 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 67, 200, 287, 669, 735 |
| BstNI | CCWGG | 1 cut(s) | 84 |
| BstNSI | RCATGY | 1 cut(s) | 912 |
| BstSCI | CCNGG | 3 cut(s) | 65, 82, 773 |
| BstSFI | CTRYAG | 1 cut(s) | 1030 |
| BstSLI | GKGCMC | 1 cut(s) | 196 |
| BstUI | CGCG | 1 cut(s) | 366 |
| BstV2I | GAAGAC | 2 cut(s) | 597, 688 |
| BstX2I | RGATCY | 4 cut(s) | 649, 813, 1129, 1342 |
| BstYI | RGATCY | 4 cut(s) | 649, 813, 1129, 1342 |
| Bsu15I | ATCGAT | 1 cut(s) | 535 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1359 |
| BsuI | GTATCC | 1 cut(s) | 299 |
| BsuRI | GGCC | 3 cut(s) | 64, 1329, 1357 |
| BsuTUI | ATCGAT | 1 cut(s) | 535 |
| BtsCI | GGATG | 7 cut(s) | 126, 281, 687, 866, 962, 1369, 1380 |
| BtsI | GCAGTG | 1 cut(s) | 1027 |
| BtsIMutI | CAGTG | 1 cut(s) | 1027 |
| BtuMI | TCGCGA | 1 cut(s) | 366 |
| Cac8I | GCNNGC | 2 cut(s) | 727, 1331 |
| CciI | TCATGA | 1 cut(s) | 511 |
| CfoI | GCGC | 1 cut(s) | 72 |
| Cfr13I | GGNCC | 3 cut(s) | 63, 135, 777 |
| ClaI | ATCGAT | 1 cut(s) | 535 |
| CseI | GACGC | 1 cut(s) | 1156 |
| CsiI | ACCWGGT | 1 cut(s) | 82 |
| Csp6I | GTAC | 3 cut(s) | 53, 916, 1024 |
| CviAII | CATG | 5 cut(s) | 512, 840, 857, 909, 930 |
| CviQI | GTAC | 3 cut(s) | 53, 916, 1024 |
| DdeI | CTNAG | 3 cut(s) | 1151, 1223, 1359 |
| DinI | GGCGCC | 1 cut(s) | 71 |
| EaeI | YGGCCR | 1 cut(s) | 1327 |
| Eam1104I | CTCTTC | 4 cut(s) | 96, 289, 310, 591 |
| EarI | CTCTTC | 4 cut(s) | 96, 289, 310, 591 |
| Ecl136II | GAGCTC | 1 cut(s) | 146 |
| Eco130I | CCWWGG | 1 cut(s) | 1209 |
| Eco24I | GRGCYC | 1 cut(s) | 148 |
| Eco32I | GATATC | 1 cut(s) | 539 |
| Eco47I | GGWCC | 2 cut(s) | 135, 777 |
| Eco53kI | GAGCTC | 1 cut(s) | 146 |
| Eco57I | CTGAAG | 1 cut(s) | 970 |
| Eco81I | CCTNAGG | 1 cut(s) | 1359 |
| EcoICRI | GAGCTC | 1 cut(s) | 146 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1284 |
| EcoRII | CCWGG | 1 cut(s) | 82 |
| EcoRV | GATATC | 1 cut(s) | 539 |
| EcoT14I | CCWWGG | 1 cut(s) | 1209 |
| EcoT38I | GRGCYC | 1 cut(s) | 148 |
| EgeI | GGCGCC | 1 cut(s) | 71 |
| EheI | GGCGCC | 1 cut(s) | 71 |
| ErhI | CCWWGG | 1 cut(s) | 1209 |
| FaeI | CATG | 5 cut(s) | 515, 843, 860, 912, 933 |
| FaqI | GGGAC | 2 cut(s) | 747, 918 |
| FatI | CATG | 5 cut(s) | 511, 839, 856, 908, 929 |
| FauNDI | CATATG | 1 cut(s) | 1352 |
| FbaI | TGATCA | 2 cut(s) | 247, 460 |
| Fnu4HI | GCNGC | 2 cut(s) | 62, 1327 |
| FokI | GGATG | 7 cut(s) | 113, 268, 674, 873, 949, 1367, 1376 |
| FriOI | GRGCYC | 1 cut(s) | 148 |
| Fsp4HI | GCNGC | 2 cut(s) | 62, 1327 |
| FspBI | CTAG | 2 cut(s) | 941, 1133 |
| GlaI | GCGC | 1 cut(s) | 71 |
| GluI | GCNGC | 2 cut(s) | 62, 1327 |
| GsaI | CCCAGC | 1 cut(s) | 1170 |
| HaeII | RGCGCY | 1 cut(s) | 73 |
| HaeIII | GGCC | 3 cut(s) | 64, 1329, 1357 |
| HapII | CCGG | 3 cut(s) | 67, 312, 774 |
| HgaI | GACGC | 1 cut(s) | 1156 |
| HhaI | GCGC | 1 cut(s) | 72 |
| Hin1I | GRCGYC | 1 cut(s) | 70 |
| Hin1II | CATG | 5 cut(s) | 515, 843, 860, 912, 933 |
| Hin6I | GCGC | 1 cut(s) | 70 |
| HinP1I | GCGC | 1 cut(s) | 70 |
| HincII | GTYRAC | 1 cut(s) | 980 |
| HindII | GTYRAC | 1 cut(s) | 980 |
| HinfI | GANTC | 6 cut(s) | 32, 104, 154, 163, 869, 1017 |
| HpaII | CCGG | 3 cut(s) | 67, 312, 774 |
| HphI | GGTGA | 3 cut(s) | 359, 386, 413 |
| Hpy166II | GTNNAC | 4 cut(s) | 896, 916, 980, 995 |
| Hpy188I | TCNGA | 6 cut(s) | 37, 109, 131, 153, 162, 619 |
| Hpy8I | GTNNAC | 4 cut(s) | 896, 916, 980, 995 |
| Hpy99I | CGWCG | 2 cut(s) | 19, 371 |
| HpyAV | CCTTC | 6 cut(s) | 101, 126, 428, 461, 491, 1085 |
| HpyCH4IV | ACGT | 1 cut(s) | 17 |
| HpyCH4V | TGCA | 6 cut(s) | 738, 803, 864, 1032, 1097, 1350 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 67, 200, 287, 669, 735 |
| HpyF3I | CTNAG | 3 cut(s) | 1151, 1223, 1359 |
| HpySE526I | ACGT | 1 cut(s) | 17 |
| Hsp92I | GRCGYC | 1 cut(s) | 70 |
| Hsp92II | CATG | 5 cut(s) | 515, 843, 860, 912, 933 |
| HspAI | GCGC | 1 cut(s) | 70 |
| KasI | GGCGCC | 1 cut(s) | 69 |
| Ksp22I | TGATCA | 2 cut(s) | 247, 460 |
| LmnI | GCTCC | 4 cut(s) | 178, 734, 776, 1175 |
| LweI | GCATC | 5 cut(s) | 48, 132, 290, 747, 851 |
| MabI | ACCWGGT | 1 cut(s) | 82 |
| MaeI | CTAG | 2 cut(s) | 941, 1133 |
| MaeII | ACGT | 1 cut(s) | 17 |
| MfeI | CAATTG | 1 cut(s) | 804 |
| MflI | RGATCY | 4 cut(s) | 649, 813, 1129, 1342 |
| MhlI | GDGCHC | 3 cut(s) | 148, 196, 1250 |
| MluCI | AATT | 6 cut(s) | 666, 747, 804, 1064, 1257, 1263 |
| Mly113I | GGCGCC | 1 cut(s) | 70 |
| MlyI | GAGTC | 2 cut(s) | 113, 878 |
| MroXI | GAANNNNTTC | 1 cut(s) | 167 |
| MseI | TTAA | 5 cut(s) | 77, 750, 819, 1125, 1298 |
| MslI | CAYNNNNRTG | 1 cut(s) | 480 |
| MspI | CCGG | 3 cut(s) | 67, 312, 774 |
| MspR9I | CCNGG | 3 cut(s) | 67, 84, 775 |
| MunI | CAATTG | 1 cut(s) | 804 |
| Mva1269I | GAATGC | 1 cut(s) | 665 |
| MvaI | CCWGG | 1 cut(s) | 84 |
| MvnI | CGCG | 1 cut(s) | 366 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 67, 200, 287, 669, 735 |
| NarI | GGCGCC | 1 cut(s) | 70 |
| NciI | CCSGG | 2 cut(s) | 67, 775 |
| NdeI | CATATG | 1 cut(s) | 1352 |
| NlaIII | CATG | 5 cut(s) | 515, 843, 860, 912, 933 |
| NlaIV | GGNNCC | 4 cut(s) | 65, 71, 195, 778 |
| NmuCI | GTSAC | 9 cut(s) | 338, 392, 419, 449, 482, 542, 874, 947, 1144 |
| NruI | TCGCGA | 1 cut(s) | 366 |
| NspI | RCATGY | 1 cut(s) | 912 |
| OliI | CACNNNNGTG | 1 cut(s) | 480 |
| PagI | TCATGA | 1 cut(s) | 511 |
| PciI | ACATGT | 1 cut(s) | 908 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 154 |
| PctI | GAATGC | 1 cut(s) | 665 |
| PdmI | GAANNNNTTC | 1 cut(s) | 167 |
| PfeI | GAWTC | 4 cut(s) | 32, 154, 163, 1017 |
| PflFI | GACNNNGTC | 1 cut(s) | 953 |
| PkrI | GCNGC | 2 cut(s) | 63, 1328 |
| PleI | GAGTC | 2 cut(s) | 112, 877 |
| PluTI | GGCGCC | 1 cut(s) | 73 |
| PpsI | GAGTC | 2 cut(s) | 112, 877 |
| PscI | ACATGT | 1 cut(s) | 908 |
| PshBI | ATTAAT | 1 cut(s) | 750 |
| PsiI | TTATAA | 1 cut(s) | 1262 |
| Psp124BI | GAGCTC | 1 cut(s) | 148 |
| Psp6I | CCWGG | 1 cut(s) | 82 |
| PspFI | CCCAGC | 1 cut(s) | 1166 |
| PspGI | CCWGG | 1 cut(s) | 82 |
| PspN4I | GGNNCC | 4 cut(s) | 65, 71, 195, 778 |
| PspPI | GGNCC | 3 cut(s) | 63, 135, 777 |
| PstI | CTGCAG | 1 cut(s) | 1034 |
| PsuI | RGATCY | 4 cut(s) | 649, 813, 1129, 1342 |
| PsyI | GACNNNGTC | 1 cut(s) | 953 |
| RruI | TCGCGA | 1 cut(s) | 366 |
| RsaI | GTAC | 3 cut(s) | 54, 917, 1025 |
| RsaNI | GTAC | 3 cut(s) | 53, 916, 1024 |
| RseI | CAYNNNNRTG | 1 cut(s) | 480 |
| SacI | GAGCTC | 1 cut(s) | 148 |
| SaqAI | TTAA | 5 cut(s) | 77, 750, 819, 1125, 1298 |
| SatI | GCNGC | 2 cut(s) | 62, 1327 |
| Sau96I | GGNCC | 3 cut(s) | 63, 135, 777 |
| ScaI | AGTACT | 1 cut(s) | 54 |
| SchI | GAGTC | 2 cut(s) | 113, 878 |
| ScrFI | CCNGG | 3 cut(s) | 67, 84, 775 |
| SduI | GDGCHC | 3 cut(s) | 148, 196, 1250 |
| SexAI | ACCWGGT | 1 cut(s) | 82 |
| SfaNI | GCATC | 5 cut(s) | 48, 132, 290, 747, 851 |
| SfcI | CTRYAG | 1 cut(s) | 1030 |
| SfoI | GGCGCC | 1 cut(s) | 71 |
| SinI | GGWCC | 2 cut(s) | 135, 777 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 480 |
| SmlI | CTYRAG | 2 cut(s) | 1109, 1334 |
| SmoI | CTYRAG | 2 cut(s) | 1109, 1334 |
| Sse9I | AATT | 6 cut(s) | 666, 747, 804, 1064, 1257, 1263 |
| SsiI | CCGC | 3 cut(s) | 61, 1053, 1326 |
| SspDI | GGCGCC | 1 cut(s) | 69 |
| SspMI | CTAG | 2 cut(s) | 941, 1133 |
| SstI | GAGCTC | 1 cut(s) | 148 |
| StyD4I | CCNGG | 3 cut(s) | 65, 82, 773 |
| StyI | CCWWGG | 1 cut(s) | 1209 |
| TaiI | ACGT | 1 cut(s) | 20 |
| TaqI | TCGA | 5 cut(s) | 14, 214, 535, 582, 1020 |
| TaqII | GACCGA | 1 cut(s) | 862 |
| TasI | AATT | 6 cut(s) | 666, 747, 804, 1064, 1257, 1263 |
| TatI | WGTACW | 2 cut(s) | 52, 915 |
| TauI | GCSGC | 2 cut(s) | 64, 1329 |
| TfiI | GAWTC | 4 cut(s) | 32, 154, 163, 1017 |
| Tru1I | TTAA | 5 cut(s) | 77, 750, 819, 1125, 1298 |
| Tru9I | TTAA | 5 cut(s) | 77, 750, 819, 1125, 1298 |
| TscAI | CASTG | 1 cut(s) | 1034 |
| TseFI | GTSAC | 9 cut(s) | 338, 392, 419, 449, 482, 542, 874, 947, 1144 |
| Tsp45I | GTSAC | 9 cut(s) | 338, 392, 419, 449, 482, 542, 874, 947, 1144 |
| TspDTI | ATGAA | 8 cut(s) | 126, 247, 342, 624, 690, 918, 951, 1104 |
| TspGWI | ACGGA | 4 cut(s) | 357, 384, 411, 561 |
| TspRI | CASTG | 1 cut(s) | 1034 |
| Tth111I | GACNNNGTC | 1 cut(s) | 953 |
| VpaK11BI | GGWCC | 2 cut(s) | 135, 777 |
| VspI | ATTAAT | 1 cut(s) | 750 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1284 |
| XapI | RAATTY | 1 cut(s) | 1064 |
| XceI | RCATGY | 1 cut(s) | 912 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 80 |
| XmnI | GAANNNNTTC | 1 cut(s) | 167 |
| XspI | CTAG | 2 cut(s) | 941, 1133 |
| ZrmI | AGTACT | 1 cut(s) | 54 |