E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 529 |
| Acc36I | ACCTGC | 1 cut(s) | 868 |
| AccIII | TCCGGA | 1 cut(s) | 53 |
| AclI | AACGTT | 2 cut(s) | 239, 633 |
| AclWI | GGATC | 2 cut(s) | 45, 58 |
| AcuI | CTGAAG | 2 cut(s) | 189, 571 |
| AfaI | GTAC | 1 cut(s) | 712 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 18, 542 |
| AgsI | TTSAA | 8 cut(s) | 73, 281, 564, 667, 740, 802, 897, 931 |
| AjnI | CCWGG | 1 cut(s) | 250 |
| AluBI | AGCT | 6 cut(s) | 209, 329, 640, 681, 768, 783 |
| AluI | AGCT | 6 cut(s) | 209, 329, 640, 681, 768, 783 |
| Alw26I | GTCTC | 1 cut(s) | 842 |
| AlwI | GGATC | 2 cut(s) | 45, 58 |
| Aor13HI | TCCGGA | 1 cut(s) | 53 |
| AoxI | GGCC | 2 cut(s) | 379, 405 |
| ApeKI | GCWGC | 4 cut(s) | 342, 462, 546, 783 |
| AseI | ATTAAT | 1 cut(s) | 354 |
| AspLEI | GCGC | 1 cut(s) | 530 |
| AspS9I | GGNCC | 2 cut(s) | 380, 405 |
| AsuC2I | CCSGG | 1 cut(s) | 404 |
| AsuHPI | GGTGA | 2 cut(s) | 487, 865 |
| AsuNHI | GCTAGC | 1 cut(s) | 209 |
| BamHI | GGATCC | 1 cut(s) | 50 |
| BauI | CACGAG | 1 cut(s) | 185 |
| BbsI | GAAGAC | 1 cut(s) | 150 |
| BbvCI | CCTCAGC | 1 cut(s) | 78 |
| BbvI | GCAGC | 4 cut(s) | 329, 449, 533, 770 |
| BccI | CCATC | 1 cut(s) | 416 |
| BciT130I | CCWGG | 1 cut(s) | 252 |
| BclI | TGATCA | 1 cut(s) | 310 |
| BcnI | CCSGG | 1 cut(s) | 404 |
| BcoDI | GTCTC | 1 cut(s) | 842 |
| BfaI | CTAG | 2 cut(s) | 210, 780 |
| BfmI | CTRYAG | 1 cut(s) | 875 |
| BfuAI | ACCTGC | 1 cut(s) | 868 |
| BisI | GCNGC | 4 cut(s) | 343, 463, 547, 784 |
| BlsI | GCNGC | 4 cut(s) | 344, 464, 548, 785 |
| Bme1390I | CCNGG | 2 cut(s) | 252, 404 |
| BmgT120I | GGNCC | 2 cut(s) | 380, 405 |
| BmiI | GGNNCC | 3 cut(s) | 52, 223, 382 |
| BmrFI | CCNGG | 2 cut(s) | 252, 404 |
| BmrI | ACTGGG | 1 cut(s) | 650 |
| BmsI | GCATC | 1 cut(s) | 94 |
| BmtI | GCTAGC | 1 cut(s) | 213 |
| BmuI | ACTGGG | 1 cut(s) | 650 |
| BpiI | GAAGAC | 1 cut(s) | 150 |
| BpmI | CTGGAG | 1 cut(s) | 198 |
| Bpu10I | CCTNAGC | 1 cut(s) | 78 |
| BpuMI | CCSGG | 1 cut(s) | 404 |
| BsaJI | CCNNGG | 1 cut(s) | 250 |
| BsaWI | WCCGGW | 2 cut(s) | 53, 472 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 18, 542 |
| Bse1I | ACTGG | 1 cut(s) | 656 |
| BseAI | TCCGGA | 1 cut(s) | 53 |
| BseBI | CCWGG | 1 cut(s) | 252 |
| BseDI | CCNNGG | 1 cut(s) | 250 |
| BseGI | GGATG | 2 cut(s) | 8, 145 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 18, 542 |
| BseMII | CTCAG | 2 cut(s) | 27, 69 |
| BseNI | ACTGG | 1 cut(s) | 656 |
| BseXI | GCAGC | 4 cut(s) | 329, 449, 533, 770 |
| BsgI | GTGCAG | 1 cut(s) | 328 |
| BshFI | GGCC | 2 cut(s) | 381, 407 |
| BsiSI | CCGG | 3 cut(s) | 54, 403, 473 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 18, 542 |
| BsmAI | GTCTC | 1 cut(s) | 842 |
| BsmI | GAATGC | 1 cut(s) | 79 |
| BsnI | GGCC | 2 cut(s) | 381, 407 |
| Bsp13I | TCCGGA | 1 cut(s) | 53 |
| Bsp143I | GATC | 2 cut(s) | 50, 310 |
| BspANI | GGCC | 2 cut(s) | 381, 407 |
| BspCNI | CTCAG | 2 cut(s) | 26, 70 |
| BspEI | TCCGGA | 1 cut(s) | 53 |
| BspLI | GGNNCC | 3 cut(s) | 52, 223, 382 |
| BspMAI | CTGCAG | 1 cut(s) | 879 |
| BspMI | ACCTGC | 1 cut(s) | 868 |
| BspOI | GCTAGC | 1 cut(s) | 213 |
| BspPI | GGATC | 2 cut(s) | 45, 58 |
| BsrI | ACTGG | 1 cut(s) | 656 |
| BssECI | CCNNGG | 1 cut(s) | 250 |
| BssMI | GATC | 2 cut(s) | 50, 310 |
| BssSI | CACGAG | 1 cut(s) | 185 |
| Bst2BI | CACGAG | 1 cut(s) | 185 |
| Bst2UI | CCWGG | 1 cut(s) | 252 |
| Bst4CI | ACNGT | 2 cut(s) | 292, 763 |
| BstC8I | GCNNGC | 4 cut(s) | 211, 331, 532, 875 |
| BstDEI | CTNAG | 2 cut(s) | 13, 78 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 16, 540 |
| BstF5I | GGATG | 2 cut(s) | 8, 145 |
| BstHHI | GCGC | 1 cut(s) | 530 |
| BstKTI | GATC | 2 cut(s) | 53, 313 |
| BstMAI | GTCTC | 1 cut(s) | 842 |
| BstMBI | GATC | 2 cut(s) | 50, 310 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 219, 273, 339, 387, 870 |
| BstNI | CCWGG | 1 cut(s) | 252 |
| BstNSI | RCATGY | 1 cut(s) | 534 |
| BstSCI | CCNGG | 2 cut(s) | 250, 402 |
| BstSFI | CTRYAG | 1 cut(s) | 875 |
| BstV1I | GCAGC | 4 cut(s) | 329, 449, 533, 770 |
| BstV2I | GAAGAC | 1 cut(s) | 150 |
| BstX2I | RGATCY | 1 cut(s) | 50 |
| BstYI | RGATCY | 1 cut(s) | 50 |
| BsuRI | GGCC | 2 cut(s) | 381, 407 |
| BtsCI | GGATG | 2 cut(s) | 8, 145 |
| BtsIMutI | CAGTG | 1 cut(s) | 311 |
| BveI | ACCTGC | 1 cut(s) | 868 |
| Cac8I | GCNNGC | 4 cut(s) | 211, 331, 532, 875 |
| CfoI | GCGC | 1 cut(s) | 530 |
| Cfr13I | GGNCC | 2 cut(s) | 380, 405 |
| Csp6I | GTAC | 1 cut(s) | 711 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 919, 954 |
| CviAII | CATG | 6 cut(s) | 63, 136, 441, 500, 531, 918 |
| CviQI | GTAC | 1 cut(s) | 711 |
| DdeI | CTNAG | 2 cut(s) | 13, 78 |
| DpnI | GATC | 2 cut(s) | 52, 312 |
| DpnII | GATC | 2 cut(s) | 50, 310 |
| Eco57I | CTGAAG | 2 cut(s) | 189, 571 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 16, 540 |
| EcoO109I | RGGNCCY | 1 cut(s) | 380 |
| EcoRII | CCWGG | 1 cut(s) | 250 |
| FaeI | CATG | 6 cut(s) | 66, 139, 444, 503, 534, 921 |
| FatI | CATG | 6 cut(s) | 62, 135, 440, 499, 530, 917 |
| FbaI | TGATCA | 1 cut(s) | 310 |
| Fnu4HI | GCNGC | 4 cut(s) | 343, 463, 547, 784 |
| FokI | GGATG | 1 cut(s) | 152 |
| Fsp4HI | GCNGC | 4 cut(s) | 343, 463, 547, 784 |
| FspBI | CTAG | 2 cut(s) | 210, 780 |
| FspI | TGCGCA | 1 cut(s) | 529 |
| GlaI | GCGC | 1 cut(s) | 529 |
| GluI | GCNGC | 4 cut(s) | 343, 463, 547, 784 |
| GsuI | CTGGAG | 1 cut(s) | 198 |
| HaeIII | GGCC | 2 cut(s) | 381, 407 |
| HapII | CCGG | 3 cut(s) | 54, 403, 473 |
| HhaI | GCGC | 1 cut(s) | 530 |
| Hin1II | CATG | 6 cut(s) | 66, 139, 444, 503, 534, 921 |
| Hin6I | GCGC | 1 cut(s) | 528 |
| HinP1I | GCGC | 1 cut(s) | 528 |
| HincII | GTYRAC | 1 cut(s) | 243 |
| HindII | GTYRAC | 1 cut(s) | 243 |
| HinfI | GANTC | 4 cut(s) | 26, 86, 425, 605 |
| HpaI | GTTAAC | 1 cut(s) | 243 |
| HpaII | CCGG | 3 cut(s) | 54, 403, 473 |
| HphI | GGTGA | 2 cut(s) | 487, 865 |
| Hpy166II | GTNNAC | 3 cut(s) | 184, 243, 713 |
| Hpy188I | TCNGA | 3 cut(s) | 16, 25, 34 |
| Hpy188III | TCNNGA | 7 cut(s) | 54, 185, 412, 422, 695, 897, 949 |
| Hpy8I | GTNNAC | 3 cut(s) | 184, 243, 713 |
| HpyAV | CCTTC | 3 cut(s) | 212, 499, 903 |
| HpyCH4III | ACNGT | 2 cut(s) | 292, 763 |
| HpyCH4IV | ACGT | 2 cut(s) | 239, 633 |
| HpyCH4V | TGCA | 5 cut(s) | 107, 345, 465, 549, 877 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 219, 273, 339, 387, 870 |
| HpyF3I | CTNAG | 2 cut(s) | 13, 78 |
| HpySE526I | ACGT | 2 cut(s) | 239, 633 |
| Hsp92II | CATG | 6 cut(s) | 66, 139, 444, 503, 534, 921 |
| HspAI | GCGC | 1 cut(s) | 528 |
| Kpn2I | TCCGGA | 1 cut(s) | 53 |
| Ksp22I | TGATCA | 1 cut(s) | 310 |
| KspAI | GTTAAC | 1 cut(s) | 243 |
| Kzo9I | GATC | 2 cut(s) | 50, 310 |
| LmnI | GCTCC | 2 cut(s) | 227, 338 |
| Lsp1109I | GCAGC | 4 cut(s) | 329, 449, 533, 770 |
| LweI | GCATC | 1 cut(s) | 94 |
| MaeI | CTAG | 2 cut(s) | 210, 780 |
| MaeII | ACGT | 2 cut(s) | 239, 633 |
| MaeIII | GTNAC | 5 cut(s) | 475, 658, 757, 817, 853 |
| MalI | GATC | 2 cut(s) | 52, 312 |
| MboI | GATC | 2 cut(s) | 50, 310 |
| MboII | GAAGA | 9 cut(s) | 50, 69, 113, 128, 131, 134, 155, 502, 552 |
| MflI | RGATCY | 1 cut(s) | 50 |
| MluCI | AATT | 3 cut(s) | 270, 351, 550 |
| MnlI | CCTC | 8 cut(s) | 22, 73, 91, 181, 379, 701, 734, 787 |
| MroI | TCCGGA | 1 cut(s) | 53 |
| MseI | TTAA | 6 cut(s) | 242, 354, 774, 788, 882, 911 |
| MspI | CCGG | 3 cut(s) | 54, 403, 473 |
| MspR9I | CCNGG | 2 cut(s) | 252, 404 |
| Mva1269I | GAATGC | 1 cut(s) | 79 |
| MvaI | CCWGG | 1 cut(s) | 252 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 219, 273, 339, 387, 870 |
| NciI | CCSGG | 1 cut(s) | 404 |
| NdeII | GATC | 2 cut(s) | 50, 310 |
| NheI | GCTAGC | 1 cut(s) | 209 |
| NlaIII | CATG | 6 cut(s) | 66, 139, 444, 503, 534, 921 |
| NlaIV | GGNNCC | 3 cut(s) | 52, 223, 382 |
| NmuCI | GTSAC | 3 cut(s) | 475, 757, 853 |
| NsbI | TGCGCA | 1 cut(s) | 529 |
| NspI | RCATGY | 1 cut(s) | 534 |
| PaeI | GCATGC | 1 cut(s) | 534 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 394 |
| PctI | GAATGC | 1 cut(s) | 79 |
| PfeI | GAWTC | 4 cut(s) | 26, 86, 425, 605 |
| PkrI | GCNGC | 4 cut(s) | 344, 464, 548, 785 |
| PshBI | ATTAAT | 1 cut(s) | 354 |
| Psp1406I | AACGTT | 2 cut(s) | 239, 633 |
| Psp6I | CCWGG | 1 cut(s) | 250 |
| PspGI | CCWGG | 1 cut(s) | 250 |
| PspN4I | GGNNCC | 3 cut(s) | 52, 223, 382 |
| PspPI | GGNCC | 2 cut(s) | 380, 405 |
| PstI | CTGCAG | 1 cut(s) | 879 |
| PsuI | RGATCY | 1 cut(s) | 50 |
| RsaI | GTAC | 1 cut(s) | 712 |
| RsaNI | GTAC | 1 cut(s) | 711 |
| SaqAI | TTAA | 6 cut(s) | 242, 354, 774, 788, 882, 911 |
| SatI | GCNGC | 4 cut(s) | 343, 463, 547, 784 |
| Sau3AI | GATC | 2 cut(s) | 50, 310 |
| Sau96I | GGNCC | 2 cut(s) | 380, 405 |
| SbfI | CCTGCAGG | 1 cut(s) | 879 |
| ScrFI | CCNGG | 2 cut(s) | 252, 404 |
| SdaI | CCTGCAGG | 1 cut(s) | 879 |
| SfaNI | GCATC | 1 cut(s) | 94 |
| SfcI | CTRYAG | 1 cut(s) | 875 |
| SphI | GCATGC | 1 cut(s) | 534 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 879 |
| Sse9I | AATT | 3 cut(s) | 270, 351, 550 |
| SspI | AATATT | 1 cut(s) | 229 |
| SspMI | CTAG | 2 cut(s) | 210, 780 |
| StyD4I | CCNGG | 2 cut(s) | 250, 402 |
| TaaI | ACNGT | 2 cut(s) | 292, 763 |
| TaiI | ACGT | 2 cut(s) | 242, 636 |
| TaqI | TCGA | 3 cut(s) | 200, 411, 694 |
| TasI | AATT | 3 cut(s) | 270, 351, 550 |
| TfiI | GAWTC | 4 cut(s) | 26, 86, 425, 605 |
| Tru1I | TTAA | 6 cut(s) | 242, 354, 774, 788, 882, 911 |
| Tru9I | TTAA | 6 cut(s) | 242, 354, 774, 788, 882, 911 |
| TscAI | CASTG | 1 cut(s) | 311 |
| TseFI | GTSAC | 3 cut(s) | 475, 757, 853 |
| TseI | GCWGC | 4 cut(s) | 342, 462, 546, 783 |
| Tsp45I | GTSAC | 3 cut(s) | 475, 757, 853 |
| TspDTI | ATGAA | 3 cut(s) | 51, 156, 510 |
| TspRI | CASTG | 1 cut(s) | 311 |
| VspI | ATTAAT | 1 cut(s) | 354 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 16, 540 |
| XceI | RCATGY | 1 cut(s) | 534 |
| XspI | CTAG | 2 cut(s) | 210, 780 |