E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 787 |
| AccIII | TCCGGA | 1 cut(s) | 697 |
| AciI | CCGC | 1 cut(s) | 177 |
| AclWI | GGATC | 2 cut(s) | 274, 423 |
| AcsI | RAATTY | 1 cut(s) | 745 |
| AdeI | CACNNNGTG | 1 cut(s) | 682 |
| AfaI | GTAC | 4 cut(s) | 87, 220, 310, 540 |
| AfeI | AGCGCT | 1 cut(s) | 473 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 385, 635 |
| AgsI | TTSAA | 3 cut(s) | 15, 269, 731 |
| AluBI | AGCT | 4 cut(s) | 11, 26, 341, 609 |
| AluI | AGCT | 4 cut(s) | 11, 26, 341, 609 |
| Alw26I | GTCTC | 1 cut(s) | 61 |
| AlwI | GGATC | 2 cut(s) | 274, 423 |
| Aor13HI | TCCGGA | 1 cut(s) | 697 |
| Aor51HI | AGCGCT | 1 cut(s) | 473 |
| ApeKI | GCWGC | 1 cut(s) | 243 |
| ApoI | RAATTY | 1 cut(s) | 745 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 455 |
| AspLEI | GCGC | 1 cut(s) | 474 |
| AspS9I | GGNCC | 1 cut(s) | 820 |
| AsuHPI | GGTGA | 3 cut(s) | 53, 715, 791 |
| AsuNHI | GCTAGC | 1 cut(s) | 7 |
| AvaII | GGWCC | 1 cut(s) | 820 |
| BaeGI | GKGCMC | 1 cut(s) | 212 |
| BbvI | GCAGC | 1 cut(s) | 255 |
| BccI | CCATC | 3 cut(s) | 38, 628, 665 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 70, 104, 487, 521 |
| BcoDI | GTCTC | 1 cut(s) | 61 |
| BfaI | CTAG | 2 cut(s) | 8, 92 |
| BfoI | RGCGCY | 1 cut(s) | 475 |
| BfuAI | ACCTGC | 1 cut(s) | 787 |
| BglII | AGATCT | 1 cut(s) | 94 |
| BisI | GCNGC | 1 cut(s) | 244 |
| BlsI | GCNGC | 1 cut(s) | 245 |
| BmcAI | AGTACT | 1 cut(s) | 87 |
| Bme18I | GGWCC | 1 cut(s) | 820 |
| BmgT120I | GGNCC | 1 cut(s) | 820 |
| BmiI | GGNNCC | 1 cut(s) | 582 |
| BmsI | GCATC | 2 cut(s) | 366, 783 |
| BmtI | GCTAGC | 1 cut(s) | 11 |
| BpmI | CTGGAG | 1 cut(s) | 612 |
| BpuEI | CTTGAG | 2 cut(s) | 21, 723 |
| BsaWI | WCCGGW | 1 cut(s) | 697 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 385, 635 |
| Bse1I | ACTGG | 2 cut(s) | 151, 406 |
| Bse3DI | GCAATG | 2 cut(s) | 258, 612 |
| BseAI | TCCGGA | 1 cut(s) | 697 |
| BseGI | GGATG | 1 cut(s) | 676 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 385, 635 |
| BseMI | GCAATG | 2 cut(s) | 258, 612 |
| BseMII | CTCAG | 1 cut(s) | 188 |
| BseNI | ACTGG | 2 cut(s) | 151, 406 |
| BseSI | GKGCMC | 1 cut(s) | 212 |
| BseXI | GCAGC | 1 cut(s) | 255 |
| BsgI | GTGCAG | 1 cut(s) | 587 |
| BsiSI | CCGG | 1 cut(s) | 698 |
| BslFI | GGGAC | 1 cut(s) | 174 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 385, 635 |
| BsmAI | GTCTC | 1 cut(s) | 61 |
| BsmFI | GGGAC | 1 cut(s) | 174 |
| BsmI | GAATGC | 1 cut(s) | 359 |
| Bsp1286I | GDGCHC | 1 cut(s) | 212 |
| Bsp13I | TCCGGA | 1 cut(s) | 697 |
| Bsp143I | GATC | 3 cut(s) | 94, 279, 415 |
| BspACI | CCGC | 1 cut(s) | 177 |
| BspCNI | CTCAG | 1 cut(s) | 189 |
| BspEI | TCCGGA | 1 cut(s) | 697 |
| BspHI | TCATGA | 1 cut(s) | 343 |
| BspLI | GGNNCC | 1 cut(s) | 582 |
| BspMI | ACCTGC | 1 cut(s) | 787 |
| BspOI | GCTAGC | 1 cut(s) | 11 |
| BspPI | GGATC | 2 cut(s) | 274, 423 |
| BsrDI | GCAATG | 2 cut(s) | 258, 612 |
| BsrI | ACTGG | 2 cut(s) | 151, 406 |
| BssMI | GATC | 3 cut(s) | 94, 279, 415 |
| Bst4CI | ACNGT | 5 cut(s) | 239, 585, 604, 682, 767 |
| Bst6I | CTCTTC | 1 cut(s) | 701 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 623 |
| BstC8I | GCNNGC | 3 cut(s) | 9, 175, 561 |
| BstDEI | CTNAG | 2 cut(s) | 63, 197 |
| BstF5I | GGATG | 1 cut(s) | 676 |
| BstH2I | RGCGCY | 1 cut(s) | 475 |
| BstHHI | GCGC | 1 cut(s) | 474 |
| BstKTI | GATC | 3 cut(s) | 97, 282, 418 |
| BstMAI | GTCTC | 1 cut(s) | 61 |
| BstMBI | GATC | 3 cut(s) | 94, 279, 415 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 183, 565, 623 |
| BstNSI | RCATGY | 1 cut(s) | 219 |
| BstSLI | GKGCMC | 1 cut(s) | 212 |
| BstV1I | GCAGC | 1 cut(s) | 255 |
| BstX2I | RGATCY | 2 cut(s) | 94, 415 |
| BstYI | RGATCY | 2 cut(s) | 94, 415 |
| BtsCI | GGATG | 1 cut(s) | 676 |
| BtsI | GCAGTG | 2 cut(s) | 570, 575 |
| BtsIMutI | CAGTG | 5 cut(s) | 158, 399, 570, 575, 678 |
| BveI | ACCTGC | 1 cut(s) | 787 |
| Cac8I | GCNNGC | 3 cut(s) | 9, 175, 561 |
| CciI | TCATGA | 1 cut(s) | 343 |
| CfoI | GCGC | 1 cut(s) | 474 |
| Cfr13I | GGNCC | 1 cut(s) | 820 |
| Csp6I | GTAC | 4 cut(s) | 86, 219, 309, 539 |
| CviAII | CATG | 7 cut(s) | 216, 285, 344, 375, 505, 542, 751 |
| CviJI | RGCY | 7 cut(s) | 11, 26, 62, 173, 341, 405, 609 |
| CviKI_1 | RGCY | 7 cut(s) | 11, 26, 62, 173, 341, 405, 609 |
| CviQI | GTAC | 4 cut(s) | 86, 219, 309, 539 |
| DdeI | CTNAG | 2 cut(s) | 63, 197 |
| DpnI | GATC | 3 cut(s) | 96, 281, 417 |
| DpnII | GATC | 3 cut(s) | 94, 279, 415 |
| DraIII | CACNNNGTG | 1 cut(s) | 682 |
| Eam1104I | CTCTTC | 1 cut(s) | 701 |
| EarI | CTCTTC | 1 cut(s) | 701 |
| Eco32I | GATATC | 2 cut(s) | 33, 103 |
| Eco47I | GGWCC | 1 cut(s) | 820 |
| Eco47III | AGCGCT | 1 cut(s) | 473 |
| EcoRV | GATATC | 2 cut(s) | 33, 103 |
| EcoT22I | ATGCAT | 1 cut(s) | 359 |
| FaeI | CATG | 7 cut(s) | 219, 288, 347, 378, 508, 545, 754 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 305, 337 |
| FaqI | GGGAC | 1 cut(s) | 174 |
| FatI | CATG | 7 cut(s) | 215, 284, 343, 374, 504, 541, 750 |
| Fnu4HI | GCNGC | 1 cut(s) | 244 |
| FokI | GGATG | 1 cut(s) | 683 |
| Fsp4HI | GCNGC | 1 cut(s) | 244 |
| FspBI | CTAG | 2 cut(s) | 8, 92 |
| GlaI | GCGC | 1 cut(s) | 473 |
| GluI | GCNGC | 1 cut(s) | 244 |
| GsuI | CTGGAG | 1 cut(s) | 612 |
| HaeII | RGCGCY | 1 cut(s) | 475 |
| HapII | CCGG | 1 cut(s) | 698 |
| HhaI | GCGC | 1 cut(s) | 474 |
| Hin1II | CATG | 7 cut(s) | 219, 288, 347, 378, 508, 545, 754 |
| Hin6I | GCGC | 1 cut(s) | 472 |
| HinP1I | GCGC | 1 cut(s) | 472 |
| HindIII | AAGCTT | 2 cut(s) | 24, 607 |
| HinfI | GANTC | 5 cut(s) | 139, 269, 335, 439, 692 |
| HpaII | CCGG | 1 cut(s) | 698 |
| HphI | GGTGA | 3 cut(s) | 53, 715, 791 |
| Hpy166II | GTNNAC | 1 cut(s) | 820 |
| Hpy188I | TCNGA | 1 cut(s) | 22 |
| Hpy188III | TCNNGA | 7 cut(s) | 98, 266, 344, 460, 629, 698, 740 |
| Hpy8I | GTNNAC | 1 cut(s) | 820 |
| HpyAV | CCTTC | 1 cut(s) | 390 |
| HpyCH4III | ACNGT | 5 cut(s) | 239, 585, 604, 682, 767 |
| HpyCH4IV | ACGT | 1 cut(s) | 294 |
| HpyCH4V | TGCA | 6 cut(s) | 215, 357, 563, 568, 617, 796 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 183, 565, 623 |
| HpyF3I | CTNAG | 2 cut(s) | 63, 197 |
| HpySE526I | ACGT | 1 cut(s) | 294 |
| Hsp92II | CATG | 7 cut(s) | 219, 288, 347, 378, 508, 545, 754 |
| HspAI | GCGC | 1 cut(s) | 472 |
| Kpn2I | TCCGGA | 1 cut(s) | 697 |
| Kzo9I | GATC | 3 cut(s) | 94, 279, 415 |
| LmnI | GCTCC | 1 cut(s) | 631 |
| LpnPI | CCDG | 9 cut(s) | 164, 225, 240, 283, 387, 445, 642, 711, 782 |
| Lsp1109I | GCAGC | 1 cut(s) | 255 |
| LweI | GCATC | 2 cut(s) | 366, 783 |
| MaeI | CTAG | 2 cut(s) | 8, 92 |
| MaeII | ACGT | 1 cut(s) | 294 |
| MaeIII | GTNAC | 4 cut(s) | 233, 598, 682, 779 |
| MalI | GATC | 3 cut(s) | 96, 281, 417 |
| MboI | GATC | 3 cut(s) | 94, 279, 415 |
| MboII | GAAGA | 4 cut(s) | 139, 352, 559, 718 |
| MflI | RGATCY | 2 cut(s) | 94, 415 |
| MhlI | GDGCHC | 1 cut(s) | 212 |
| MluCI | AATT | 4 cut(s) | 15, 487, 528, 745 |
| MlyI | GAGTC | 3 cut(s) | 148, 344, 433 |
| MmeI | TCCRAC | 1 cut(s) | 768 |
| MnlI | CCTC | 7 cut(s) | 41, 116, 511, 694, 702, 747, 771 |
| Mph1103I | ATGCAT | 1 cut(s) | 359 |
| MroI | TCCGGA | 1 cut(s) | 697 |
| MroXI | GAANNNNTTC | 1 cut(s) | 455 |
| MseI | TTAA | 2 cut(s) | 468, 531 |
| MspI | CCGG | 1 cut(s) | 698 |
| Mva1269I | GAATGC | 1 cut(s) | 359 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 183, 565, 623 |
| NdeII | GATC | 3 cut(s) | 94, 279, 415 |
| NheI | GCTAGC | 1 cut(s) | 7 |
| NlaIII | CATG | 7 cut(s) | 219, 288, 347, 378, 508, 545, 754 |
| NlaIV | GGNNCC | 1 cut(s) | 582 |
| NmeAIII | GCCGAG | 1 cut(s) | 56 |
| NmuCI | GTSAC | 4 cut(s) | 233, 598, 682, 779 |
| NsiI | ATGCAT | 1 cut(s) | 359 |
| NspI | RCATGY | 1 cut(s) | 219 |
| PagI | TCATGA | 1 cut(s) | 343 |
| PctI | GAATGC | 1 cut(s) | 359 |
| PdmI | GAANNNNTTC | 1 cut(s) | 455 |
| PfeI | GAWTC | 2 cut(s) | 269, 692 |
| PkrI | GCNGC | 1 cut(s) | 245 |
| PleI | GAGTC | 3 cut(s) | 147, 343, 433 |
| PpsI | GAGTC | 3 cut(s) | 147, 343, 433 |
| PspN4I | GGNNCC | 1 cut(s) | 582 |
| PspPI | GGNCC | 1 cut(s) | 820 |
| PsuI | RGATCY | 2 cut(s) | 94, 415 |
| RsaI | GTAC | 4 cut(s) | 87, 220, 310, 540 |
| RsaNI | GTAC | 4 cut(s) | 86, 219, 309, 539 |
| SaqAI | TTAA | 2 cut(s) | 468, 531 |
| SatI | GCNGC | 1 cut(s) | 244 |
| Sau3AI | GATC | 3 cut(s) | 94, 279, 415 |
| Sau96I | GGNCC | 1 cut(s) | 820 |
| ScaI | AGTACT | 1 cut(s) | 87 |
| SchI | GAGTC | 3 cut(s) | 148, 344, 433 |
| SduI | GDGCHC | 1 cut(s) | 212 |
| SfaNI | GCATC | 2 cut(s) | 366, 783 |
| SinI | GGWCC | 1 cut(s) | 820 |
| SmlI | CTYRAG | 2 cut(s) | 36, 738 |
| SmoI | CTYRAG | 2 cut(s) | 36, 738 |
| Sse9I | AATT | 4 cut(s) | 15, 487, 528, 745 |
| SsiI | CCGC | 1 cut(s) | 177 |
| SspI | AATATT | 2 cut(s) | 454, 466 |
| SspMI | CTAG | 2 cut(s) | 8, 92 |
| TaaI | ACNGT | 5 cut(s) | 239, 585, 604, 682, 767 |
| TaiI | ACGT | 1 cut(s) | 297 |
| TaqI | TCGA | 2 cut(s) | 108, 810 |
| TaqII | GACCGA | 1 cut(s) | 376 |
| TasI | AATT | 4 cut(s) | 15, 487, 528, 745 |
| TatI | WGTACW | 2 cut(s) | 85, 218 |
| TfiI | GAWTC | 2 cut(s) | 269, 692 |
| Tru1I | TTAA | 2 cut(s) | 468, 531 |
| Tru9I | TTAA | 2 cut(s) | 468, 531 |
| TscAI | CASTG | 5 cut(s) | 158, 406, 570, 575, 685 |
| TseFI | GTSAC | 4 cut(s) | 233, 598, 682, 779 |
| TseI | GCWGC | 1 cut(s) | 243 |
| Tsp45I | GTSAC | 4 cut(s) | 233, 598, 682, 779 |
| TspDTI | ATGAA | 5 cut(s) | 360, 372, 601, 705, 739 |
| TspRI | CASTG | 5 cut(s) | 158, 406, 570, 575, 685 |
| VpaK11BI | GGWCC | 1 cut(s) | 820 |
| XapI | RAATTY | 1 cut(s) | 745 |
| XceI | RCATGY | 1 cut(s) | 219 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 158 |
| XmnI | GAANNNNTTC | 1 cut(s) | 455 |
| XspI | CTAG | 2 cut(s) | 8, 92 |
| ZrmI | AGTACT | 1 cut(s) | 87 |
| Zsp2I | ATGCAT | 1 cut(s) | 359 |