E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1088 |
| Acc65I | GGTACC | 1 cut(s) | 830 |
| AccB1I | GGYRCC | 1 cut(s) | 830 |
| AccII | CGCG | 1 cut(s) | 1036 |
| AciI | CCGC | 4 cut(s) | 121, 163, 1034, 1065 |
| AclWI | GGATC | 4 cut(s) | 102, 106, 154, 529 |
| AcoI | YGGCCR | 1 cut(s) | 537 |
| AcsI | RAATTY | 1 cut(s) | 279 |
| AfaI | GTAC | 2 cut(s) | 590, 832 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 535, 676, 1078 |
| AflIII | ACRYGT | 1 cut(s) | 641 |
| AgsI | TTSAA | 6 cut(s) | 217, 314, 413, 896, 907, 1059 |
| AjnI | CCWGG | 1 cut(s) | 382 |
| AleI | CACNNNNGTG | 1 cut(s) | 852 |
| AluBI | AGCT | 2 cut(s) | 461, 888 |
| AluI | AGCT | 2 cut(s) | 461, 888 |
| Alw26I | GTCTC | 2 cut(s) | 214, 697 |
| AlwI | GGATC | 4 cut(s) | 102, 106, 154, 529 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 26, 143 |
| Ama87I | CYCGRG | 1 cut(s) | 1073 |
| AoxI | GGCC | 1 cut(s) | 537 |
| ApeKI | GCWGC | 2 cut(s) | 594, 888 |
| ApoI | RAATTY | 1 cut(s) | 279 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 279 |
| Asp718I | GGTACC | 1 cut(s) | 830 |
| AspLEI | GCGC | 2 cut(s) | 479, 1089 |
| AsuC2I | CCSGG | 1 cut(s) | 285 |
| AsuHPI | GGTGA | 3 cut(s) | 356, 619, 1061 |
| AsuII | TTCGAA | 1 cut(s) | 988 |
| AvaI | CYCGRG | 1 cut(s) | 1073 |
| BanI | GGYRCC | 1 cut(s) | 830 |
| BbsI | GAAGAC | 2 cut(s) | 81, 424 |
| BbvI | GCAGC | 2 cut(s) | 581, 875 |
| BccI | CCATC | 3 cut(s) | 437, 548, 1072 |
| BceAI | ACGGC | 1 cut(s) | 607 |
| BciT130I | CCWGG | 1 cut(s) | 384 |
| BcnI | CCSGG | 1 cut(s) | 285 |
| BcoDI | GTCTC | 2 cut(s) | 214, 697 |
| BfaI | CTAG | 3 cut(s) | 657, 866, 1008 |
| BfmI | CTRYAG | 1 cut(s) | 995 |
| BisI | GCNGC | 2 cut(s) | 595, 889 |
| BlsI | GCNGC | 2 cut(s) | 596, 890 |
| Bme1390I | CCNGG | 2 cut(s) | 285, 384 |
| BmeT110I | CYCGRG | 1 cut(s) | 1073 |
| BmiI | GGNNCC | 1 cut(s) | 832 |
| BmrFI | CCNGG | 2 cut(s) | 285, 384 |
| BmrI | ACTGGG | 1 cut(s) | 1011 |
| BmuI | ACTGGG | 1 cut(s) | 1011 |
| BpiI | GAAGAC | 2 cut(s) | 81, 424 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 677, 709, 1031, 1063 |
| Bpu10I | CCTNAGC | 1 cut(s) | 884 |
| Bpu14I | TTCGAA | 1 cut(s) | 988 |
| BpuEI | CTTGAG | 1 cut(s) | 544 |
| BpuMI | CCSGG | 1 cut(s) | 285 |
| BsaI | GGTCTC | 1 cut(s) | 214 |
| BsaJI | CCNNGG | 2 cut(s) | 382, 702 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 535, 676, 1078 |
| Bse118I | RCCGGY | 1 cut(s) | 117 |
| Bse1I | ACTGG | 2 cut(s) | 436, 1017 |
| BseBI | CCWGG | 1 cut(s) | 384 |
| BseDI | CCNNGG | 2 cut(s) | 382, 702 |
| BseGI | GGATG | 2 cut(s) | 193, 829 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 535, 676, 1078 |
| BseMII | CTCAG | 3 cut(s) | 144, 192, 223 |
| BseNI | ACTGG | 2 cut(s) | 436, 1017 |
| BseXI | GCAGC | 2 cut(s) | 581, 875 |
| BsgI | GTGCAG | 1 cut(s) | 580 |
| Bsh1236I | CGCG | 1 cut(s) | 1036 |
| BshFI | GGCC | 1 cut(s) | 539 |
| BshNI | GGYRCC | 1 cut(s) | 830 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1073 |
| BsiSI | CCGG | 2 cut(s) | 118, 284 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 535, 676, 1078 |
| BsmAI | GTCTC | 2 cut(s) | 214, 697 |
| BsmI | GAATGC | 2 cut(s) | 891, 984 |
| BsnI | GGCC | 1 cut(s) | 539 |
| Bso31I | GGTCTC | 1 cut(s) | 214 |
| BsoBI | CYCGRG | 1 cut(s) | 1073 |
| Bsp119I | TTCGAA | 1 cut(s) | 988 |
| Bsp143I | GATC | 6 cut(s) | 42, 94, 111, 159, 521, 862 |
| Bsp19I | CCATGG | 1 cut(s) | 702 |
| BspACI | CCGC | 4 cut(s) | 121, 163, 1034, 1065 |
| BspANI | GGCC | 1 cut(s) | 539 |
| BspCNI | CTCAG | 3 cut(s) | 143, 191, 222 |
| BspFNI | CGCG | 1 cut(s) | 1036 |
| BspLI | GGNNCC | 1 cut(s) | 832 |
| BspMAI | CTGCAG | 1 cut(s) | 999 |
| BspPI | GGATC | 4 cut(s) | 102, 106, 154, 529 |
| BspT104I | TTCGAA | 1 cut(s) | 988 |
| BspT107I | GGYRCC | 1 cut(s) | 830 |
| BspTNI | GGTCTC | 1 cut(s) | 214 |
| BsrFI | RCCGGY | 1 cut(s) | 117 |
| BsrI | ACTGG | 2 cut(s) | 436, 1017 |
| BssAI | RCCGGY | 1 cut(s) | 117 |
| BssECI | CCNNGG | 2 cut(s) | 382, 702 |
| BssMI | GATC | 6 cut(s) | 42, 94, 111, 159, 521, 862 |
| BssT1I | CCWWGG | 1 cut(s) | 702 |
| Bst2UI | CCWGG | 1 cut(s) | 384 |
| Bst4CI | ACNGT | 5 cut(s) | 80, 350, 424, 530, 969 |
| BstBI | TTCGAA | 1 cut(s) | 988 |
| BstC8I | GCNNGC | 3 cut(s) | 463, 995, 999 |
| BstDEI | CTNAG | 6 cut(s) | 130, 178, 209, 248, 374, 884 |
| BstDSI | CCRYGG | 1 cut(s) | 702 |
| BstEII | GGTNACC | 3 cut(s) | 344, 367, 1067 |
| BstF5I | GGATG | 2 cut(s) | 193, 829 |
| BstFNI | CGCG | 1 cut(s) | 1036 |
| BstHHI | GCGC | 2 cut(s) | 479, 1089 |
| BstKTI | GATC | 6 cut(s) | 45, 97, 114, 162, 524, 865 |
| BstMAI | GTCTC | 2 cut(s) | 214, 697 |
| BstMBI | GATC | 6 cut(s) | 42, 94, 111, 159, 521, 862 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 405, 888, 990 |
| BstNI | CCWGG | 1 cut(s) | 384 |
| BstNSI | RCATGY | 1 cut(s) | 645 |
| BstPI | GGTNACC | 3 cut(s) | 344, 367, 1067 |
| BstSCI | CCNGG | 2 cut(s) | 283, 382 |
| BstSFI | CTRYAG | 1 cut(s) | 995 |
| BstUI | CGCG | 1 cut(s) | 1036 |
| BstV1I | GCAGC | 2 cut(s) | 581, 875 |
| BstV2I | GAAGAC | 2 cut(s) | 81, 424 |
| BstX2I | RGATCY | 1 cut(s) | 94 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 443 |
| BstYI | RGATCY | 1 cut(s) | 94 |
| BsuRI | GGCC | 1 cut(s) | 539 |
| BtgI | CCRYGG | 1 cut(s) | 702 |
| BtsCI | GGATG | 2 cut(s) | 193, 829 |
| BtsI | GCAGTG | 1 cut(s) | 597 |
| BtsIMutI | CAGTG | 3 cut(s) | 443, 597, 978 |
| Cac8I | GCNNGC | 3 cut(s) | 463, 995, 999 |
| CaiI | CAGNNNCTG | 2 cut(s) | 26, 143 |
| CfoI | GCGC | 2 cut(s) | 479, 1089 |
| Cfr10I | RCCGGY | 1 cut(s) | 117 |
| Csp6I | GTAC | 2 cut(s) | 589, 831 |
| CviAII | CATG | 4 cut(s) | 632, 642, 703, 734 |
| CviQI | GTAC | 2 cut(s) | 589, 831 |
| DdeI | CTNAG | 6 cut(s) | 130, 178, 209, 248, 374, 884 |
| DpnI | GATC | 6 cut(s) | 44, 96, 113, 161, 523, 864 |
| DpnII | GATC | 6 cut(s) | 42, 94, 111, 159, 521, 862 |
| EaeI | YGGCCR | 1 cut(s) | 537 |
| EciI | GGCGGA | 2 cut(s) | 136, 152 |
| Eco130I | CCWWGG | 1 cut(s) | 702 |
| Eco31I | GGTCTC | 1 cut(s) | 214 |
| Eco88I | CYCGRG | 1 cut(s) | 1073 |
| Eco91I | GGTNACC | 3 cut(s) | 344, 367, 1067 |
| EcoO65I | GGTNACC | 3 cut(s) | 344, 367, 1067 |
| EcoRII | CCWGG | 1 cut(s) | 382 |
| EcoT14I | CCWWGG | 1 cut(s) | 702 |
| ErhI | CCWWGG | 1 cut(s) | 702 |
| FaeI | CATG | 4 cut(s) | 635, 645, 706, 737 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 553, 585, 899, 931 |
| FatI | CATG | 4 cut(s) | 631, 641, 702, 733 |
| FauI | CCCGC | 1 cut(s) | 1041 |
| Fnu4HI | GCNGC | 2 cut(s) | 595, 889 |
| FokI | GGATG | 2 cut(s) | 200, 836 |
| Fsp4HI | GCNGC | 2 cut(s) | 595, 889 |
| FspAI | RTGCGCAY | 1 cut(s) | 1088 |
| FspBI | CTAG | 3 cut(s) | 657, 866, 1008 |
| FspI | TGCGCA | 1 cut(s) | 1088 |
| GlaI | GCGC | 2 cut(s) | 478, 1088 |
| GluI | GCNGC | 2 cut(s) | 595, 889 |
| HaeIII | GGCC | 1 cut(s) | 539 |
| HapII | CCGG | 2 cut(s) | 118, 284 |
| HhaI | GCGC | 2 cut(s) | 479, 1089 |
| Hin1II | CATG | 4 cut(s) | 635, 645, 706, 737 |
| Hin6I | GCGC | 2 cut(s) | 477, 1087 |
| HinP1I | GCGC | 2 cut(s) | 477, 1087 |
| HpaII | CCGG | 2 cut(s) | 118, 284 |
| HphI | GGTGA | 3 cut(s) | 356, 619, 1061 |
| Hpy166II | GTNNAC | 1 cut(s) | 556 |
| Hpy188I | TCNGA | 8 cut(s) | 22, 28, 91, 133, 139, 145, 181, 187 |
| Hpy188III | TCNNGA | 7 cut(s) | 83, 98, 217, 544, 757, 815, 1075 |
| Hpy8I | GTNNAC | 1 cut(s) | 556 |
| HpyAV | CCTTC | 3 cut(s) | 510, 541, 1034 |
| HpyCH4III | ACNGT | 5 cut(s) | 80, 350, 424, 530, 969 |
| HpyCH4V | TGCA | 5 cut(s) | 597, 647, 746, 891, 997 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 405, 888, 990 |
| HpyF3I | CTNAG | 6 cut(s) | 130, 178, 209, 248, 374, 884 |
| Hsp92II | CATG | 4 cut(s) | 635, 645, 706, 737 |
| HspAI | GCGC | 2 cut(s) | 477, 1087 |
| KpnI | GGTACC | 1 cut(s) | 834 |
| Kzo9I | GATC | 6 cut(s) | 42, 94, 111, 159, 521, 862 |
| Lsp1109I | GCAGC | 2 cut(s) | 581, 875 |
| MaeI | CTAG | 3 cut(s) | 657, 866, 1008 |
| MaeIII | GTNAC | 7 cut(s) | 344, 350, 367, 377, 607, 973, 1067 |
| MalI | GATC | 6 cut(s) | 44, 96, 113, 161, 523, 864 |
| MboI | GATC | 6 cut(s) | 42, 94, 111, 159, 521, 862 |
| MflI | RGATCY | 1 cut(s) | 94 |
| MluCI | AATT | 4 cut(s) | 279, 402, 962, 1060 |
| MlyI | GAGTC | 1 cut(s) | 551 |
| MnlI | CCTC | 7 cut(s) | 85, 234, 780, 904, 968, 1023, 1082 |
| MroXI | GAANNNNTTC | 1 cut(s) | 279 |
| MseI | TTAA | 1 cut(s) | 11 |
| MslI | CAYNNNNRTG | 2 cut(s) | 852, 1094 |
| MspI | CCGG | 2 cut(s) | 118, 284 |
| MspR9I | CCNGG | 2 cut(s) | 285, 384 |
| Mva1269I | GAATGC | 2 cut(s) | 891, 984 |
| MvaI | CCWGG | 1 cut(s) | 384 |
| MvnI | CGCG | 1 cut(s) | 1036 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 405, 888, 990 |
| NciI | CCSGG | 1 cut(s) | 285 |
| NcoI | CCATGG | 1 cut(s) | 702 |
| NdeII | GATC | 6 cut(s) | 42, 94, 111, 159, 521, 862 |
| NlaIII | CATG | 4 cut(s) | 635, 645, 706, 737 |
| NlaIV | GGNNCC | 1 cut(s) | 832 |
| NmeAIII | GCCGAG | 2 cut(s) | 30, 515 |
| NmuCI | GTSAC | 5 cut(s) | 344, 377, 607, 973, 1067 |
| NsbI | TGCGCA | 1 cut(s) | 1088 |
| NspI | RCATGY | 1 cut(s) | 645 |
| NspV | TTCGAA | 1 cut(s) | 988 |
| OliI | CACNNNNGTG | 1 cut(s) | 852 |
| PaeR7I | CTCGAG | 1 cut(s) | 1073 |
| PciI | ACATGT | 1 cut(s) | 641 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 549 |
| PctI | GAATGC | 2 cut(s) | 891, 984 |
| PdmI | GAANNNNTTC | 1 cut(s) | 279 |
| PflFI | GACNNNGTC | 1 cut(s) | 225 |
| PfoI | TCCNGGA | 1 cut(s) | 283 |
| PkrI | GCNGC | 2 cut(s) | 596, 890 |
| PleI | GAGTC | 1 cut(s) | 551 |
| PpsI | GAGTC | 1 cut(s) | 551 |
| PscI | ACATGT | 1 cut(s) | 641 |
| Psp6I | CCWGG | 1 cut(s) | 382 |
| PspEI | GGTNACC | 3 cut(s) | 344, 367, 1067 |
| PspGI | CCWGG | 1 cut(s) | 382 |
| PspN4I | GGNNCC | 1 cut(s) | 832 |
| PstI | CTGCAG | 1 cut(s) | 999 |
| PstNI | CAGNNNCTG | 2 cut(s) | 26, 143 |
| PsuI | RGATCY | 1 cut(s) | 94 |
| PsyI | GACNNNGTC | 1 cut(s) | 225 |
| RsaI | GTAC | 2 cut(s) | 590, 832 |
| RsaNI | GTAC | 2 cut(s) | 589, 831 |
| RseI | CAYNNNNRTG | 2 cut(s) | 852, 1094 |
| SaqAI | TTAA | 1 cut(s) | 11 |
| SatI | GCNGC | 2 cut(s) | 595, 889 |
| Sau3AI | GATC | 6 cut(s) | 42, 94, 111, 159, 521, 862 |
| SbfI | CCTGCAGG | 1 cut(s) | 999 |
| SchI | GAGTC | 1 cut(s) | 551 |
| ScrFI | CCNGG | 2 cut(s) | 285, 384 |
| SdaI | CCTGCAGG | 1 cut(s) | 999 |
| SfcI | CTRYAG | 1 cut(s) | 995 |
| Sfr274I | CTCGAG | 1 cut(s) | 1073 |
| SfuI | TTCGAA | 1 cut(s) | 988 |
| SgrAI | CRCCGGYG | 1 cut(s) | 117 |
| SlaI | CTCGAG | 1 cut(s) | 1073 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 852, 1094 |
| SmlI | CTYRAG | 2 cut(s) | 559, 1073 |
| SmoI | CTYRAG | 2 cut(s) | 559, 1073 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 999 |
| Sse9I | AATT | 4 cut(s) | 279, 402, 962, 1060 |
| SsiI | CCGC | 4 cut(s) | 121, 163, 1034, 1065 |
| SspI | AATATT | 1 cut(s) | 361 |
| SspMI | CTAG | 3 cut(s) | 657, 866, 1008 |
| StyD4I | CCNGG | 2 cut(s) | 283, 382 |
| StyI | CCWWGG | 1 cut(s) | 702 |
| TaaI | ACNGT | 5 cut(s) | 80, 350, 424, 530, 969 |
| TaqI | TCGA | 6 cut(s) | 486, 524, 543, 814, 988, 1074 |
| TasI | AATT | 4 cut(s) | 279, 402, 962, 1060 |
| Tru1I | TTAA | 1 cut(s) | 11 |
| Tru9I | TTAA | 1 cut(s) | 11 |
| TscAI | CASTG | 3 cut(s) | 443, 604, 978 |
| TseFI | GTSAC | 5 cut(s) | 344, 377, 607, 973, 1067 |
| TseI | GCWGC | 2 cut(s) | 594, 888 |
| Tsp45I | GTSAC | 5 cut(s) | 344, 377, 607, 973, 1067 |
| TspDTI | ATGAA | 5 cut(s) | 642, 648, 771, 829, 907 |
| TspRI | CASTG | 3 cut(s) | 443, 604, 978 |
| Tth111I | GACNNNGTC | 1 cut(s) | 225 |
| XapI | RAATTY | 1 cut(s) | 279 |
| XceI | RCATGY | 1 cut(s) | 645 |
| XhoI | CTCGAG | 1 cut(s) | 1073 |
| XmnI | GAANNNNTTC | 1 cut(s) | 279 |
| XspI | CTAG | 3 cut(s) | 657, 866, 1008 |