E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 8 |
| AccII | CGCG | 1 cut(s) | 14 |
| AccIII | TCCGGA | 1 cut(s) | 534 |
| AciI | CCGC | 2 cut(s) | 12, 770 |
| AclWI | GGATC | 4 cut(s) | 52, 141, 313, 462 |
| AcoI | YGGCCR | 1 cut(s) | 271 |
| AcsI | RAATTY | 1 cut(s) | 778 |
| AdeI | CACNNNGTG | 1 cut(s) | 715 |
| AfaI | GTAC | 3 cut(s) | 259, 461, 677 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 35, 424 |
| AflII | CTTAAG | 2 cut(s) | 526, 749 |
| AgsI | TTSAA | 3 cut(s) | 308, 764, 850 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 383, 415 |
| AluBI | AGCT | 4 cut(s) | 95, 170, 444, 642 |
| AluI | AGCT | 4 cut(s) | 95, 170, 444, 642 |
| Alw26I | GTCTC | 2 cut(s) | 64, 96 |
| AlwI | GGATC | 4 cut(s) | 52, 141, 313, 462 |
| Aor13HI | TCCGGA | 1 cut(s) | 534 |
| AoxI | GGCC | 1 cut(s) | 271 |
| ApeKI | GCWGC | 3 cut(s) | 92, 282, 642 |
| ApoI | RAATTY | 1 cut(s) | 778 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 571, 603 |
| AseI | ATTAAT | 1 cut(s) | 501 |
| AspLEI | GCGC | 2 cut(s) | 413, 652 |
| AsuC2I | CCSGG | 1 cut(s) | 30 |
| AsuHPI | GGTGA | 3 cut(s) | 720, 748, 824 |
| BalI | TGGCCA | 1 cut(s) | 273 |
| BanI | GGYRCC | 1 cut(s) | 8 |
| BbvI | GCAGC | 3 cut(s) | 104, 269, 629 |
| BccI | CCATC | 3 cut(s) | 191, 661, 698 |
| BceAI | ACGGC | 1 cut(s) | 601 |
| BcnI | CCSGG | 1 cut(s) | 30 |
| BcoDI | GTCTC | 2 cut(s) | 64, 96 |
| BfaI | CTAG | 2 cut(s) | 84, 441 |
| BfmI | CTRYAG | 1 cut(s) | 283 |
| BfrI | CTTAAG | 2 cut(s) | 526, 749 |
| BisI | GCNGC | 4 cut(s) | 12, 93, 283, 643 |
| BlsI | GCNGC | 4 cut(s) | 13, 94, 284, 644 |
| BmcAI | AGTACT | 1 cut(s) | 677 |
| Bme1390I | CCNGG | 1 cut(s) | 30 |
| BmiI | GGNNCC | 2 cut(s) | 10, 615 |
| BmrFI | CCNGG | 1 cut(s) | 30 |
| BmsI | GCATC | 1 cut(s) | 79 |
| BpmI | CTGGAG | 2 cut(s) | 158, 645 |
| BpuMI | CCSGG | 1 cut(s) | 30 |
| Bsa29I | ATCGAT | 1 cut(s) | 311 |
| BsaBI | GATNNNNATC | 2 cut(s) | 317, 542 |
| BsaWI | WCCGGW | 1 cut(s) | 534 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 35, 424 |
| Bse1I | ACTGG | 1 cut(s) | 190 |
| Bse3DI | GCAATG | 3 cut(s) | 297, 406, 645 |
| Bse8I | GATNNNNATC | 2 cut(s) | 317, 542 |
| BseAI | TCCGGA | 1 cut(s) | 534 |
| BseCI | ATCGAT | 1 cut(s) | 311 |
| BseGI | GGATG | 1 cut(s) | 543 |
| BseJI | GATNNNNATC | 2 cut(s) | 317, 542 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 35, 424 |
| BseMI | GCAATG | 3 cut(s) | 297, 406, 645 |
| BseNI | ACTGG | 1 cut(s) | 190 |
| BseXI | GCAGC | 3 cut(s) | 104, 269, 629 |
| BsgI | GTGCAG | 1 cut(s) | 620 |
| Bsh1236I | CGCG | 1 cut(s) | 14 |
| BshFI | GGCC | 1 cut(s) | 273 |
| BshNI | GGYRCC | 1 cut(s) | 8 |
| BshVI | ATCGAT | 1 cut(s) | 311 |
| BsiSI | CCGG | 2 cut(s) | 30, 535 |
| BslFI | GGGAC | 2 cut(s) | 138, 213 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 35, 424 |
| BsmAI | GTCTC | 2 cut(s) | 64, 96 |
| BsmBI | CGTCTC | 1 cut(s) | 96 |
| BsmFI | GGGAC | 2 cut(s) | 138, 213 |
| BsmI | GAATGC | 2 cut(s) | 54, 398 |
| BsnI | GGCC | 1 cut(s) | 273 |
| Bsp13I | TCCGGA | 1 cut(s) | 534 |
| Bsp1407I | TGTACA | 1 cut(s) | 459 |
| Bsp143I | GATC | 5 cut(s) | 44, 133, 312, 318, 454 |
| BspACI | CCGC | 2 cut(s) | 12, 770 |
| BspANI | GGCC | 1 cut(s) | 273 |
| BspDI | ATCGAT | 1 cut(s) | 311 |
| BspEI | TCCGGA | 1 cut(s) | 534 |
| BspFNI | CGCG | 1 cut(s) | 14 |
| BspHI | TCATGA | 1 cut(s) | 382 |
| BspLI | GGNNCC | 2 cut(s) | 10, 615 |
| BspMAI | CTGCAG | 1 cut(s) | 287 |
| BspPI | GGATC | 4 cut(s) | 52, 141, 313, 462 |
| BspT107I | GGYRCC | 1 cut(s) | 8 |
| BspTI | CTTAAG | 2 cut(s) | 526, 749 |
| BsrDI | GCAATG | 3 cut(s) | 297, 406, 645 |
| BsrGI | TGTACA | 1 cut(s) | 459 |
| BsrI | ACTGG | 1 cut(s) | 190 |
| BssMI | GATC | 5 cut(s) | 44, 133, 312, 318, 454 |
| Bst4CI | ACNGT | 4 cut(s) | 637, 700, 715, 800 |
| Bst6I | CTCTTC | 1 cut(s) | 734 |
| BstAFI | CTTAAG | 2 cut(s) | 526, 749 |
| BstAUI | TGTACA | 1 cut(s) | 459 |
| BstC8I | GCNNGC | 1 cut(s) | 415 |
| BstDEI | CTNAG | 2 cut(s) | 96, 108 |
| BstF5I | GGATG | 1 cut(s) | 543 |
| BstFNI | CGCG | 1 cut(s) | 14 |
| BstHHI | GCGC | 2 cut(s) | 413, 652 |
| BstKTI | GATC | 5 cut(s) | 47, 136, 315, 321, 457 |
| BstMAI | GTCTC | 2 cut(s) | 64, 96 |
| BstMBI | GATC | 5 cut(s) | 44, 133, 312, 318, 454 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 279, 419 |
| BstNSI | RCATGY | 1 cut(s) | 400 |
| BstSCI | CCNGG | 1 cut(s) | 28 |
| BstSFI | CTRYAG | 1 cut(s) | 283 |
| BstUI | CGCG | 1 cut(s) | 14 |
| BstV1I | GCAGC | 3 cut(s) | 104, 269, 629 |
| BstX2I | RGATCY | 3 cut(s) | 44, 133, 454 |
| BstYI | RGATCY | 3 cut(s) | 44, 133, 454 |
| Bsu15I | ATCGAT | 1 cut(s) | 311 |
| BsuRI | GGCC | 1 cut(s) | 273 |
| BsuTUI | ATCGAT | 1 cut(s) | 311 |
| BtsCI | GGATG | 1 cut(s) | 543 |
| BtsI | GCAGTG | 2 cut(s) | 603, 608 |
| BtsIMutI | CAGTG | 5 cut(s) | 197, 603, 608, 696, 711 |
| Cac8I | GCNNGC | 1 cut(s) | 415 |
| CciI | TCATGA | 1 cut(s) | 382 |
| CfoI | GCGC | 2 cut(s) | 413, 652 |
| ClaI | ATCGAT | 1 cut(s) | 311 |
| CseI | GACGC | 2 cut(s) | 113, 826 |
| Csp6I | GTAC | 3 cut(s) | 258, 460, 676 |
| CviAII | CATG | 3 cut(s) | 383, 397, 784 |
| CviJI | RGCY | 7 cut(s) | 95, 170, 212, 273, 444, 616, 642 |
| CviKI_1 | RGCY | 7 cut(s) | 95, 170, 212, 273, 444, 616, 642 |
| CviQI | GTAC | 3 cut(s) | 258, 460, 676 |
| DdeI | CTNAG | 2 cut(s) | 96, 108 |
| DpnI | GATC | 5 cut(s) | 46, 135, 314, 320, 456 |
| DpnII | GATC | 5 cut(s) | 44, 133, 312, 318, 454 |
| DraIII | CACNNNGTG | 1 cut(s) | 715 |
| EaeI | YGGCCR | 1 cut(s) | 271 |
| Eam1104I | CTCTTC | 1 cut(s) | 734 |
| EarI | CTCTTC | 1 cut(s) | 734 |
| EcoT22I | ATGCAT | 1 cut(s) | 398 |
| Esp3I | CGTCTC | 1 cut(s) | 96 |
| FaeI | CATG | 3 cut(s) | 386, 400, 787 |
| FaqI | GGGAC | 2 cut(s) | 138, 213 |
| FatI | CATG | 3 cut(s) | 382, 396, 783 |
| FauI | CCCGC | 1 cut(s) | 777 |
| FauNDI | CATATG | 2 cut(s) | 483, 682 |
| Fnu4HI | GCNGC | 4 cut(s) | 12, 93, 283, 643 |
| FokI | GGATG | 1 cut(s) | 550 |
| Fsp4HI | GCNGC | 4 cut(s) | 12, 93, 283, 643 |
| FspBI | CTAG | 2 cut(s) | 84, 441 |
| GlaI | GCGC | 2 cut(s) | 412, 651 |
| GluI | GCNGC | 4 cut(s) | 12, 93, 283, 643 |
| GsuI | CTGGAG | 2 cut(s) | 158, 645 |
| HaeIII | GGCC | 1 cut(s) | 273 |
| HapII | CCGG | 2 cut(s) | 30, 535 |
| HgaI | GACGC | 2 cut(s) | 113, 826 |
| HhaI | GCGC | 2 cut(s) | 413, 652 |
| Hin1II | CATG | 3 cut(s) | 386, 400, 787 |
| Hin6I | GCGC | 2 cut(s) | 411, 650 |
| HinP1I | GCGC | 2 cut(s) | 411, 650 |
| HincII | GTYRAC | 1 cut(s) | 378 |
| HindII | GTYRAC | 1 cut(s) | 378 |
| HindIII | AAGCTT | 1 cut(s) | 168 |
| HinfI | GANTC | 5 cut(s) | 23, 142, 308, 583, 790 |
| HpaI | GTTAAC | 1 cut(s) | 378 |
| HpaII | CCGG | 2 cut(s) | 30, 535 |
| HphI | GGTGA | 3 cut(s) | 720, 748, 824 |
| Hpy166II | GTNNAC | 3 cut(s) | 258, 378, 462 |
| Hpy188I | TCNGA | 1 cut(s) | 330 |
| Hpy188III | TCNNGA | 8 cut(s) | 137, 146, 305, 316, 383, 535, 662, 841 |
| Hpy8I | GTNNAC | 3 cut(s) | 258, 378, 462 |
| HpyAV | CCTTC | 1 cut(s) | 29 |
| HpyCH4III | ACNGT | 4 cut(s) | 637, 700, 715, 800 |
| HpyCH4IV | ACGT | 1 cut(s) | 506 |
| HpyCH4V | TGCA | 9 cut(s) | 92, 285, 348, 396, 422, 432, 596, 601, 645 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 279, 419 |
| HpyF3I | CTNAG | 2 cut(s) | 96, 108 |
| HpySE526I | ACGT | 1 cut(s) | 506 |
| Hsp92II | CATG | 3 cut(s) | 386, 400, 787 |
| HspAI | GCGC | 2 cut(s) | 411, 650 |
| Kpn2I | TCCGGA | 1 cut(s) | 534 |
| KspAI | GTTAAC | 1 cut(s) | 378 |
| Kzo9I | GATC | 5 cut(s) | 44, 133, 312, 318, 454 |
| LmnI | GCTCC | 1 cut(s) | 613 |
| Lsp1109I | GCAGC | 3 cut(s) | 104, 269, 629 |
| LweI | GCATC | 1 cut(s) | 79 |
| MaeI | CTAG | 2 cut(s) | 84, 441 |
| MaeII | ACGT | 1 cut(s) | 506 |
| MaeIII | GTNAC | 2 cut(s) | 515, 631 |
| MalI | GATC | 5 cut(s) | 46, 135, 314, 320, 456 |
| MboI | GATC | 5 cut(s) | 44, 133, 312, 318, 454 |
| MboII | GAAGA | 5 cut(s) | 72, 178, 210, 648, 751 |
| MflI | RGATCY | 3 cut(s) | 44, 133, 454 |
| MlsI | TGGCCA | 1 cut(s) | 273 |
| MluCI | AATT | 5 cut(s) | 39, 177, 433, 665, 778 |
| MluNI | TGGCCA | 1 cut(s) | 273 |
| MmeI | TCCRAC | 1 cut(s) | 801 |
| MnlI | CCTC | 3 cut(s) | 727, 735, 822 |
| Mox20I | TGGCCA | 1 cut(s) | 273 |
| Mph1103I | ATGCAT | 1 cut(s) | 398 |
| MroI | TCCGGA | 1 cut(s) | 534 |
| MscI | TGGCCA | 1 cut(s) | 273 |
| MseI | TTAA | 5 cut(s) | 377, 501, 527, 668, 750 |
| Msp20I | TGGCCA | 1 cut(s) | 273 |
| MspCI | CTTAAG | 2 cut(s) | 526, 749 |
| MspI | CCGG | 2 cut(s) | 30, 535 |
| MspR9I | CCNGG | 1 cut(s) | 30 |
| Mva1269I | GAATGC | 2 cut(s) | 54, 398 |
| MvnI | CGCG | 1 cut(s) | 14 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 279, 419 |
| NciI | CCSGG | 1 cut(s) | 30 |
| NdeI | CATATG | 2 cut(s) | 483, 682 |
| NdeII | GATC | 5 cut(s) | 44, 133, 312, 318, 454 |
| NlaIII | CATG | 3 cut(s) | 386, 400, 787 |
| NlaIV | GGNNCC | 2 cut(s) | 10, 615 |
| NmuCI | GTSAC | 1 cut(s) | 515 |
| NsiI | ATGCAT | 1 cut(s) | 398 |
| NspI | RCATGY | 1 cut(s) | 400 |
| PagI | TCATGA | 1 cut(s) | 382 |
| PctI | GAATGC | 2 cut(s) | 54, 398 |
| PfeI | GAWTC | 5 cut(s) | 23, 142, 308, 583, 790 |
| PkrI | GCNGC | 4 cut(s) | 13, 94, 284, 644 |
| PshBI | ATTAAT | 1 cut(s) | 501 |
| PspN4I | GGNNCC | 2 cut(s) | 10, 615 |
| PstI | CTGCAG | 1 cut(s) | 287 |
| PsuI | RGATCY | 3 cut(s) | 44, 133, 454 |
| RsaI | GTAC | 3 cut(s) | 259, 461, 677 |
| RsaNI | GTAC | 3 cut(s) | 258, 460, 676 |
| SaqAI | TTAA | 5 cut(s) | 377, 501, 527, 668, 750 |
| SatI | GCNGC | 4 cut(s) | 12, 93, 283, 643 |
| Sau3AI | GATC | 5 cut(s) | 44, 133, 312, 318, 454 |
| ScaI | AGTACT | 1 cut(s) | 677 |
| ScrFI | CCNGG | 1 cut(s) | 30 |
| SetI | ASST | 7 cut(s) | 97, 172, 346, 446, 509, 644, 738 |
| SfaNI | GCATC | 1 cut(s) | 79 |
| SfcI | CTRYAG | 1 cut(s) | 283 |
| SmlI | CTYRAG | 2 cut(s) | 526, 749 |
| SmoI | CTYRAG | 2 cut(s) | 526, 749 |
| Sse9I | AATT | 5 cut(s) | 39, 177, 433, 665, 778 |
| SsiI | CCGC | 2 cut(s) | 12, 770 |
| SspMI | CTAG | 2 cut(s) | 84, 441 |
| StyD4I | CCNGG | 1 cut(s) | 28 |
| TaaI | ACNGT | 4 cut(s) | 637, 700, 715, 800 |
| TaiI | ACGT | 1 cut(s) | 509 |
| TaqI | TCGA | 4 cut(s) | 119, 147, 311, 702 |
| TasI | AATT | 5 cut(s) | 39, 177, 433, 665, 778 |
| TatI | WGTACW | 3 cut(s) | 257, 459, 675 |
| TauI | GCSGC | 1 cut(s) | 14 |
| TfiI | GAWTC | 5 cut(s) | 23, 142, 308, 583, 790 |
| Tru1I | TTAA | 5 cut(s) | 377, 501, 527, 668, 750 |
| Tru9I | TTAA | 5 cut(s) | 377, 501, 527, 668, 750 |
| TscAI | CASTG | 5 cut(s) | 197, 603, 608, 703, 718 |
| TseFI | GTSAC | 1 cut(s) | 515 |
| TseI | GCWGC | 3 cut(s) | 92, 282, 642 |
| Tsp45I | GTSAC | 1 cut(s) | 515 |
| TspDTI | ATGAA | 4 cut(s) | 399, 499, 554, 772 |
| TspGWI | ACGGA | 1 cut(s) | 584 |
| TspRI | CASTG | 5 cut(s) | 197, 603, 608, 703, 718 |
| Vha464I | CTTAAG | 2 cut(s) | 526, 749 |
| VspI | ATTAAT | 1 cut(s) | 501 |
| XapI | RAATTY | 1 cut(s) | 778 |
| XceI | RCATGY | 1 cut(s) | 400 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 197 |
| XspI | CTAG | 2 cut(s) | 84, 441 |
| ZrmI | AGTACT | 1 cut(s) | 677 |
| Zsp2I | ATGCAT | 1 cut(s) | 398 |