E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1079 |
| Acc65I | GGTACC | 1 cut(s) | 824 |
| AccB1I | GGYRCC | 1 cut(s) | 824 |
| AccBSI | CCGCTC | 1 cut(s) | 208 |
| AccII | CGCG | 2 cut(s) | 1030, 1130 |
| AciI | CCGC | 6 cut(s) | 121, 163, 206, 1028, 1150, 1220 |
| AclWI | GGATC | 3 cut(s) | 102, 106, 154 |
| AcoI | YGGCCR | 1 cut(s) | 531 |
| AcsI | RAATTY | 1 cut(s) | 273 |
| AfaI | GTAC | 2 cut(s) | 584, 826 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 529, 670, 671, 1069, 1251, 1336 |
| AflIII | ACRYGT | 1 cut(s) | 635 |
| AgsI | TTSAA | 8 cut(s) | 217, 248, 278, 308, 407, 890, 901, 1053 |
| AhlI | ACTAGT | 1 cut(s) | 1133 |
| AjnI | CCWGG | 1 cut(s) | 376 |
| AleI | CACNNNNGTG | 1 cut(s) | 846 |
| AluBI | AGCT | 2 cut(s) | 455, 882 |
| AluI | AGCT | 2 cut(s) | 455, 882 |
| Alw21I | GWGCWC | 2 cut(s) | 1102, 1179 |
| Alw26I | GTCTC | 3 cut(s) | 214, 691, 1210 |
| Alw44I | GTGCAC | 1 cut(s) | 1175 |
| AlwI | GGATC | 3 cut(s) | 102, 106, 154 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 26, 143 |
| Ama87I | CYCGRG | 2 cut(s) | 169, 1064 |
| AoxI | GGCC | 3 cut(s) | 531, 1327, 1343 |
| ApaLI | GTGCAC | 1 cut(s) | 1175 |
| ApeKI | GCWGC | 1 cut(s) | 588 |
| ApoI | RAATTY | 1 cut(s) | 273 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 273 |
| Asp718I | GGTACC | 1 cut(s) | 824 |
| AspLEI | GCGC | 2 cut(s) | 1080, 1130 |
| AsuC2I | CCSGG | 1 cut(s) | 1274 |
| AsuHPI | GGTGA | 2 cut(s) | 350, 613 |
| AsuII | TTCGAA | 1 cut(s) | 982 |
| AvaI | CYCGRG | 2 cut(s) | 169, 1064 |
| BaeGI | GKGCMC | 1 cut(s) | 1179 |
| BanI | GGYRCC | 1 cut(s) | 824 |
| BbsI | GAAGAC | 2 cut(s) | 81, 418 |
| Bbv12I | GWGCWC | 2 cut(s) | 1102, 1179 |
| BbvI | GCAGC | 1 cut(s) | 575 |
| BccI | CCATC | 4 cut(s) | 431, 542, 1063, 1310 |
| BceAI | ACGGC | 2 cut(s) | 601, 1195 |
| BciT130I | CCWGG | 1 cut(s) | 378 |
| BcnI | CCSGG | 1 cut(s) | 1274 |
| BcoDI | GTCTC | 3 cut(s) | 214, 691, 1210 |
| BcuI | ACTAGT | 1 cut(s) | 1133 |
| BfaI | CTAG | 6 cut(s) | 651, 860, 1002, 1134, 1196, 1223 |
| BfmI | CTRYAG | 1 cut(s) | 989 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 513 |
| BisI | GCNGC | 2 cut(s) | 589, 1151 |
| BlsI | GCNGC | 2 cut(s) | 590, 1152 |
| Bme1390I | CCNGG | 2 cut(s) | 378, 1274 |
| BmeT110I | CYCGRG | 2 cut(s) | 169, 1064 |
| BmiI | GGNNCC | 1 cut(s) | 826 |
| BmrFI | CCNGG | 2 cut(s) | 378, 1274 |
| BpiI | GAAGAC | 2 cut(s) | 81, 418 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 671, 703, 1025, 1057 |
| Bpu10I | CCTNAGC | 2 cut(s) | 209, 878 |
| Bpu14I | TTCGAA | 1 cut(s) | 982 |
| BpuEI | CTTGAG | 2 cut(s) | 538, 1210 |
| BpuMI | CCSGG | 1 cut(s) | 1274 |
| BsaI | GGTCTC | 2 cut(s) | 214, 1210 |
| BsaJI | CCNNGG | 3 cut(s) | 376, 696, 1324 |
| BsaWI | WCCGGW | 2 cut(s) | 598, 1348 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 529, 670, 671, 1069, 1251, 1336 |
| Bse118I | RCCGGY | 2 cut(s) | 117, 708 |
| Bse1I | ACTGG | 2 cut(s) | 430, 1011 |
| BseBI | CCWGG | 1 cut(s) | 378 |
| BseDI | CCNNGG | 3 cut(s) | 376, 696, 1324 |
| BseGI | GGATG | 3 cut(s) | 193, 823, 1321 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 529, 670, 671, 1069, 1251, 1336 |
| BseMII | CTCAG | 3 cut(s) | 144, 192, 223 |
| BseNI | ACTGG | 2 cut(s) | 430, 1011 |
| BseRI | GAGGAG | 4 cut(s) | 1181, 1184, 1214, 1217 |
| BseSI | GKGCMC | 1 cut(s) | 1179 |
| BseXI | GCAGC | 1 cut(s) | 575 |
| BseYI | CCCAGC | 1 cut(s) | 1336 |
| BsgI | GTGCAG | 1 cut(s) | 574 |
| Bsh1236I | CGCG | 2 cut(s) | 1030, 1130 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1213 |
| BshFI | GGCC | 3 cut(s) | 533, 1329, 1345 |
| BshNI | GGYRCC | 1 cut(s) | 824 |
| BsiEI | CGRYCG | 1 cut(s) | 1213 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1102, 1179 |
| BsiHKCI | CYCGRG | 2 cut(s) | 169, 1064 |
| BsiSI | CCGG | 5 cut(s) | 118, 599, 709, 1273, 1349 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 529, 670, 671, 1069, 1251, 1336 |
| BsmAI | GTCTC | 3 cut(s) | 214, 691, 1210 |
| BsmI | GAATGC | 1 cut(s) | 978 |
| BsnI | GGCC | 3 cut(s) | 533, 1329, 1345 |
| Bso31I | GGTCTC | 2 cut(s) | 214, 1210 |
| BsoBI | CYCGRG | 2 cut(s) | 169, 1064 |
| Bsp119I | TTCGAA | 1 cut(s) | 982 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1102, 1179 |
| Bsp143I | GATC | 6 cut(s) | 42, 94, 111, 159, 856, 1210 |
| Bsp19I | CCATGG | 2 cut(s) | 696, 1324 |
| BspACI | CCGC | 6 cut(s) | 121, 163, 206, 1028, 1150, 1220 |
| BspANI | GGCC | 3 cut(s) | 533, 1329, 1345 |
| BspCNI | CTCAG | 3 cut(s) | 143, 191, 222 |
| BspFNI | CGCG | 2 cut(s) | 1030, 1130 |
| BspLI | GGNNCC | 1 cut(s) | 826 |
| BspMAI | CTGCAG | 1 cut(s) | 993 |
| BspPI | GGATC | 3 cut(s) | 102, 106, 154 |
| BspQI | GCTCTTC | 1 cut(s) | 1090 |
| BspT104I | TTCGAA | 1 cut(s) | 982 |
| BspT107I | GGYRCC | 1 cut(s) | 824 |
| BspTNI | GGTCTC | 2 cut(s) | 214, 1210 |
| BsrBI | CCGCTC | 1 cut(s) | 208 |
| BsrFI | RCCGGY | 2 cut(s) | 117, 708 |
| BsrI | ACTGG | 2 cut(s) | 430, 1011 |
| BssAI | RCCGGY | 2 cut(s) | 117, 708 |
| BssECI | CCNNGG | 3 cut(s) | 376, 696, 1324 |
| BssMI | GATC | 6 cut(s) | 42, 94, 111, 159, 856, 1210 |
| BssT1I | CCWWGG | 2 cut(s) | 696, 1324 |
| Bst2UI | CCWGG | 1 cut(s) | 378 |
| Bst4CI | ACNGT | 4 cut(s) | 80, 418, 524, 963 |
| Bst6I | CTCTTC | 1 cut(s) | 1090 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1183 |
| BstBI | TTCGAA | 1 cut(s) | 982 |
| BstC8I | GCNNGC | 5 cut(s) | 457, 989, 993, 1184, 1270 |
| BstDEI | CTNAG | 6 cut(s) | 58, 130, 178, 209, 368, 878 |
| BstDSI | CCRYGG | 2 cut(s) | 696, 1324 |
| BstEII | GGTNACC | 2 cut(s) | 338, 361 |
| BstF5I | GGATG | 3 cut(s) | 193, 823, 1321 |
| BstFNI | CGCG | 2 cut(s) | 1030, 1130 |
| BstHHI | GCGC | 2 cut(s) | 1080, 1130 |
| BstKTI | GATC | 6 cut(s) | 45, 97, 114, 162, 859, 1213 |
| BstMAI | GTCTC | 3 cut(s) | 214, 691, 1210 |
| BstMBI | GATC | 6 cut(s) | 42, 94, 111, 159, 856, 1210 |
| BstMCI | CGRYCG | 1 cut(s) | 1213 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 399, 513, 984, 1097, 1183, 1342 |
| BstNI | CCWGG | 1 cut(s) | 378 |
| BstNSI | RCATGY | 2 cut(s) | 639, 1186 |
| BstPI | GGTNACC | 2 cut(s) | 338, 361 |
| BstSCI | CCNGG | 2 cut(s) | 376, 1272 |
| BstSFI | CTRYAG | 1 cut(s) | 989 |
| BstSLI | GKGCMC | 1 cut(s) | 1179 |
| BstUI | CGCG | 2 cut(s) | 1030, 1130 |
| BstV1I | GCAGC | 1 cut(s) | 575 |
| BstV2I | GAAGAC | 2 cut(s) | 81, 418 |
| BstX2I | RGATCY | 1 cut(s) | 94 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 437 |
| BstYI | RGATCY | 1 cut(s) | 94 |
| BsuRI | GGCC | 3 cut(s) | 533, 1329, 1345 |
| BtgI | CCRYGG | 2 cut(s) | 696, 1324 |
| BtsCI | GGATG | 3 cut(s) | 193, 823, 1321 |
| BtsI | GCAGTG | 1 cut(s) | 591 |
| BtsIMutI | CAGTG | 3 cut(s) | 437, 591, 972 |
| Cac8I | GCNNGC | 5 cut(s) | 457, 989, 993, 1184, 1270 |
| CaiI | CAGNNNCTG | 2 cut(s) | 26, 143 |
| CfoI | GCGC | 2 cut(s) | 1080, 1130 |
| Cfr10I | RCCGGY | 2 cut(s) | 117, 708 |
| CseI | GACGC | 1 cut(s) | 942 |
| Csp6I | GTAC | 2 cut(s) | 583, 825 |
| CviAII | CATG | 8 cut(s) | 462, 626, 636, 697, 728, 1183, 1265, 1325 |
| CviQI | GTAC | 2 cut(s) | 583, 825 |
| DdeI | CTNAG | 6 cut(s) | 58, 130, 178, 209, 368, 878 |
| DpnI | GATC | 6 cut(s) | 44, 96, 113, 161, 858, 1212 |
| DpnII | GATC | 6 cut(s) | 42, 94, 111, 159, 856, 1210 |
| EaeI | YGGCCR | 1 cut(s) | 531 |
| Eam1104I | CTCTTC | 1 cut(s) | 1090 |
| EarI | CTCTTC | 1 cut(s) | 1090 |
| EciI | GGCGGA | 2 cut(s) | 136, 152 |
| Eco130I | CCWWGG | 2 cut(s) | 696, 1324 |
| Eco31I | GGTCTC | 2 cut(s) | 214, 1210 |
| Eco88I | CYCGRG | 2 cut(s) | 169, 1064 |
| Eco91I | GGTNACC | 2 cut(s) | 338, 361 |
| EcoO65I | GGTNACC | 2 cut(s) | 338, 361 |
| EcoRII | CCWGG | 1 cut(s) | 376 |
| EcoT14I | CCWWGG | 2 cut(s) | 696, 1324 |
| ErhI | CCWWGG | 2 cut(s) | 696, 1324 |
| FaeI | CATG | 8 cut(s) | 465, 629, 639, 700, 731, 1186, 1268, 1328 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 547, 579, 893, 925 |
| FatI | CATG | 8 cut(s) | 461, 625, 635, 696, 727, 1182, 1264, 1324 |
| FauI | CCCGC | 1 cut(s) | 1035 |
| Fnu4HI | GCNGC | 2 cut(s) | 589, 1151 |
| FokI | GGATG | 3 cut(s) | 200, 830, 1328 |
| Fsp4HI | GCNGC | 2 cut(s) | 589, 1151 |
| FspAI | RTGCGCAY | 1 cut(s) | 1079 |
| FspBI | CTAG | 6 cut(s) | 651, 860, 1002, 1134, 1196, 1223 |
| FspI | TGCGCA | 1 cut(s) | 1079 |
| GlaI | GCGC | 2 cut(s) | 1079, 1129 |
| GluI | GCNGC | 2 cut(s) | 589, 1151 |
| GsaI | CCCAGC | 1 cut(s) | 1340 |
| HaeIII | GGCC | 3 cut(s) | 533, 1329, 1345 |
| HapII | CCGG | 5 cut(s) | 118, 599, 709, 1273, 1349 |
| HgaI | GACGC | 1 cut(s) | 942 |
| HhaI | GCGC | 2 cut(s) | 1080, 1130 |
| Hin1II | CATG | 8 cut(s) | 465, 629, 639, 700, 731, 1186, 1268, 1328 |
| Hin6I | GCGC | 2 cut(s) | 1078, 1128 |
| HinP1I | GCGC | 2 cut(s) | 1078, 1128 |
| HincII | GTYRAC | 1 cut(s) | 1162 |
| HindII | GTYRAC | 1 cut(s) | 1162 |
| HpaII | CCGG | 5 cut(s) | 118, 599, 709, 1273, 1349 |
| HphI | GGTGA | 2 cut(s) | 350, 613 |
| Hpy166II | GTNNAC | 3 cut(s) | 550, 1162, 1177 |
| Hpy188III | TCNNGA | 9 cut(s) | 83, 98, 217, 538, 751, 809, 1066, 1189, 1196 |
| Hpy8I | GTNNAC | 3 cut(s) | 550, 1162, 1177 |
| HpyAV | CCTTC | 6 cut(s) | 251, 504, 535, 1017, 1028, 1335 |
| HpyCH4III | ACNGT | 4 cut(s) | 80, 418, 524, 963 |
| HpyCH4V | TGCA | 7 cut(s) | 471, 591, 641, 740, 991, 1177, 1288 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 399, 513, 984, 1097, 1183, 1342 |
| HpyF3I | CTNAG | 6 cut(s) | 58, 130, 178, 209, 368, 878 |
| Hsp92II | CATG | 8 cut(s) | 465, 629, 639, 700, 731, 1186, 1268, 1328 |
| HspAI | GCGC | 2 cut(s) | 1078, 1128 |
| KpnI | GGTACC | 1 cut(s) | 828 |
| Kzo9I | GATC | 6 cut(s) | 42, 94, 111, 159, 856, 1210 |
| LguI | GCTCTTC | 1 cut(s) | 1090 |
| LmnI | GCTCC | 1 cut(s) | 601 |
| Lsp1109I | GCAGC | 1 cut(s) | 575 |
| MaeI | CTAG | 6 cut(s) | 651, 860, 1002, 1134, 1196, 1223 |
| MaeIII | GTNAC | 5 cut(s) | 338, 361, 371, 601, 967 |
| MalI | GATC | 6 cut(s) | 44, 96, 113, 161, 858, 1212 |
| MbiI | CCGCTC | 1 cut(s) | 208 |
| MboI | GATC | 6 cut(s) | 42, 94, 111, 159, 856, 1210 |
| MflI | RGATCY | 1 cut(s) | 94 |
| MhlI | GDGCHC | 2 cut(s) | 1102, 1179 |
| MluCI | AATT | 4 cut(s) | 273, 396, 956, 1054 |
| MlyI | GAGTC | 2 cut(s) | 545, 1201 |
| MmeI | TCCRAC | 1 cut(s) | 1258 |
| MroXI | GAANNNNTTC | 1 cut(s) | 273 |
| MseI | TTAA | 1 cut(s) | 11 |
| MslI | CAYNNNNRTG | 2 cut(s) | 846, 1085 |
| MspI | CCGG | 5 cut(s) | 118, 599, 709, 1273, 1349 |
| MspR9I | CCNGG | 2 cut(s) | 378, 1274 |
| Mva1269I | GAATGC | 1 cut(s) | 978 |
| MvaI | CCWGG | 1 cut(s) | 378 |
| MvnI | CGCG | 2 cut(s) | 1030, 1130 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 399, 513, 984, 1097, 1183, 1342 |
| NciI | CCSGG | 1 cut(s) | 1274 |
| NcoI | CCATGG | 2 cut(s) | 696, 1324 |
| NdeII | GATC | 6 cut(s) | 42, 94, 111, 159, 856, 1210 |
| NlaIII | CATG | 8 cut(s) | 465, 629, 639, 700, 731, 1186, 1268, 1328 |
| NlaIV | GGNNCC | 1 cut(s) | 826 |
| NmeAIII | GCCGAG | 3 cut(s) | 30, 509, 1270 |
| NmuCI | GTSAC | 4 cut(s) | 338, 371, 601, 967 |
| NsbI | TGCGCA | 1 cut(s) | 1079 |
| NspI | RCATGY | 2 cut(s) | 639, 1186 |
| NspV | TTCGAA | 1 cut(s) | 982 |
| OliI | CACNNNNGTG | 1 cut(s) | 846 |
| PaeI | GCATGC | 1 cut(s) | 1186 |
| PaeR7I | CTCGAG | 1 cut(s) | 1064 |
| PciI | ACATGT | 1 cut(s) | 635 |
| PciSI | GCTCTTC | 1 cut(s) | 1090 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 321, 543 |
| PctI | GAATGC | 1 cut(s) | 978 |
| PdmI | GAANNNNTTC | 1 cut(s) | 273 |
| PflFI | GACNNNGTC | 1 cut(s) | 225 |
| PkrI | GCNGC | 2 cut(s) | 590, 1152 |
| Ple19I | CGATCG | 1 cut(s) | 1213 |
| PleI | GAGTC | 2 cut(s) | 545, 1200 |
| PpsI | GAGTC | 2 cut(s) | 545, 1200 |
| PscI | ACATGT | 1 cut(s) | 635 |
| Psp6I | CCWGG | 1 cut(s) | 376 |
| PspEI | GGTNACC | 2 cut(s) | 338, 361 |
| PspFI | CCCAGC | 1 cut(s) | 1336 |
| PspGI | CCWGG | 1 cut(s) | 376 |
| PspN4I | GGNNCC | 1 cut(s) | 826 |
| PstI | CTGCAG | 1 cut(s) | 993 |
| PstNI | CAGNNNCTG | 2 cut(s) | 26, 143 |
| PsuI | RGATCY | 1 cut(s) | 94 |
| PsyI | GACNNNGTC | 1 cut(s) | 225 |
| PvuI | CGATCG | 1 cut(s) | 1213 |
| RsaI | GTAC | 2 cut(s) | 584, 826 |
| RsaNI | GTAC | 2 cut(s) | 583, 825 |
| RseI | CAYNNNNRTG | 2 cut(s) | 846, 1085 |
| SapI | GCTCTTC | 1 cut(s) | 1090 |
| SaqAI | TTAA | 1 cut(s) | 11 |
| SatI | GCNGC | 2 cut(s) | 589, 1151 |
| Sau3AI | GATC | 6 cut(s) | 42, 94, 111, 159, 856, 1210 |
| SbfI | CCTGCAGG | 1 cut(s) | 993 |
| SchI | GAGTC | 2 cut(s) | 545, 1201 |
| ScrFI | CCNGG | 2 cut(s) | 378, 1274 |
| SdaI | CCTGCAGG | 1 cut(s) | 993 |
| SduI | GDGCHC | 2 cut(s) | 1102, 1179 |
| SfcI | CTRYAG | 1 cut(s) | 989 |
| Sfr274I | CTCGAG | 1 cut(s) | 1064 |
| SfuI | TTCGAA | 1 cut(s) | 982 |
| SgrAI | CRCCGGYG | 1 cut(s) | 117 |
| SlaI | CTCGAG | 1 cut(s) | 1064 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 846, 1085 |
| SmlI | CTYRAG | 3 cut(s) | 553, 1064, 1189 |
| SmoI | CTYRAG | 3 cut(s) | 553, 1064, 1189 |
| SpeI | ACTAGT | 1 cut(s) | 1133 |
| SphI | GCATGC | 1 cut(s) | 1186 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 993 |
| Sse9I | AATT | 4 cut(s) | 273, 396, 956, 1054 |
| SsiI | CCGC | 6 cut(s) | 121, 163, 206, 1028, 1150, 1220 |
| SspI | AATATT | 1 cut(s) | 355 |
| SspMI | CTAG | 6 cut(s) | 651, 860, 1002, 1134, 1196, 1223 |
| StyD4I | CCNGG | 2 cut(s) | 376, 1272 |
| StyI | CCWWGG | 2 cut(s) | 696, 1324 |
| TaaI | ACNGT | 4 cut(s) | 80, 418, 524, 963 |
| TaqI | TCGA | 5 cut(s) | 518, 537, 808, 982, 1065 |
| TasI | AATT | 4 cut(s) | 273, 396, 956, 1054 |
| TauI | GCSGC | 1 cut(s) | 1153 |
| Tru1I | TTAA | 1 cut(s) | 11 |
| Tru9I | TTAA | 1 cut(s) | 11 |
| TscAI | CASTG | 3 cut(s) | 437, 598, 972 |
| TseFI | GTSAC | 4 cut(s) | 338, 371, 601, 967 |
| TseI | GCWGC | 1 cut(s) | 588 |
| Tsp45I | GTSAC | 4 cut(s) | 338, 371, 601, 967 |
| TspDTI | ATGAA | 6 cut(s) | 636, 642, 765, 823, 901, 1253 |
| TspRI | CASTG | 3 cut(s) | 437, 598, 972 |
| Tth111I | GACNNNGTC | 1 cut(s) | 225 |
| VneI | GTGCAC | 1 cut(s) | 1175 |
| XapI | RAATTY | 1 cut(s) | 273 |
| XbaI | TCTAGA | 1 cut(s) | 1195 |
| XceI | RCATGY | 2 cut(s) | 639, 1186 |
| XhoI | CTCGAG | 1 cut(s) | 1064 |
| XmnI | GAANNNNTTC | 1 cut(s) | 273 |
| XspI | CTAG | 6 cut(s) | 651, 860, 1002, 1134, 1196, 1223 |