E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 346 |
| AcuI | CTGAAG | 1 cut(s) | 69 |
| AfaI | GTAC | 4 cut(s) | 52, 101, 149, 223 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 65, 437 |
| AgsI | TTSAA | 6 cut(s) | 9, 112, 206, 254, 290, 320 |
| AjnI | CCWGG | 1 cut(s) | 293 |
| AluBI | AGCT | 2 cut(s) | 89, 96 |
| AluI | AGCT | 2 cut(s) | 89, 96 |
| Alw26I | GTCTC | 1 cut(s) | 85 |
| ApoI | RAATTY | 1 cut(s) | 346 |
| AsuHPI | GGTGA | 1 cut(s) | 479 |
| BanII | GRGCYC | 1 cut(s) | 313 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 92, 124, 170, 202 |
| BciT130I | CCWGG | 1 cut(s) | 295 |
| BcoDI | GTCTC | 1 cut(s) | 85 |
| BfaI | CTAG | 2 cut(s) | 12, 90 |
| BfmI | CTRYAG | 1 cut(s) | 543 |
| Bme1390I | CCNGG | 1 cut(s) | 295 |
| BmiI | GGNNCC | 1 cut(s) | 312 |
| BmrFI | CCNGG | 1 cut(s) | 295 |
| BmsI | GCATC | 1 cut(s) | 388 |
| BpuEI | CTTGAG | 2 cut(s) | 338, 461 |
| BsaJI | CCNNGG | 1 cut(s) | 293 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 65, 437 |
| BseBI | CCWGG | 1 cut(s) | 295 |
| BseDI | CCNNGG | 1 cut(s) | 293 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 65, 437 |
| BsgI | GTGCAG | 1 cut(s) | 279 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 65, 437 |
| BsmAI | GTCTC | 1 cut(s) | 85 |
| BsmI | GAATGC | 1 cut(s) | 40 |
| Bsp1286I | GDGCHC | 1 cut(s) | 313 |
| Bsp1407I | TGTACA | 3 cut(s) | 50, 99, 147 |
| Bsp143I | GATC | 2 cut(s) | 469, 480 |
| BspHI | TCATGA | 1 cut(s) | 300 |
| BspLI | GGNNCC | 1 cut(s) | 312 |
| BsrGI | TGTACA | 3 cut(s) | 50, 99, 147 |
| BssECI | CCNNGG | 1 cut(s) | 293 |
| BssMI | GATC | 2 cut(s) | 469, 480 |
| Bst2UI | CCWGG | 1 cut(s) | 295 |
| Bst4CI | ACNGT | 1 cut(s) | 547 |
| Bst6I | CTCTTC | 1 cut(s) | 88 |
| BstAUI | TGTACA | 3 cut(s) | 50, 99, 147 |
| BstC8I | GCNNGC | 2 cut(s) | 34, 38 |
| BstKTI | GATC | 2 cut(s) | 472, 483 |
| BstMAI | GTCTC | 1 cut(s) | 85 |
| BstMBI | GATC | 2 cut(s) | 469, 480 |
| BstNI | CCWGG | 1 cut(s) | 295 |
| BstNSI | RCATGY | 3 cut(s) | 36, 40, 57 |
| BstSCI | CCNGG | 1 cut(s) | 293 |
| BstSFI | CTRYAG | 1 cut(s) | 543 |
| Cac8I | GCNNGC | 2 cut(s) | 34, 38 |
| CciI | TCATGA | 1 cut(s) | 300 |
| CseI | GACGC | 1 cut(s) | 371 |
| Csp6I | GTAC | 4 cut(s) | 51, 100, 148, 222 |
| CviAII | CATG | 8 cut(s) | 23, 33, 37, 54, 122, 301, 447, 555 |
| CviJI | RGCY | 5 cut(s) | 89, 96, 311, 475, 502 |
| CviKI_1 | RGCY | 5 cut(s) | 89, 96, 311, 475, 502 |
| CviQI | GTAC | 4 cut(s) | 51, 100, 148, 222 |
| DpnI | GATC | 2 cut(s) | 471, 482 |
| DpnII | GATC | 2 cut(s) | 469, 480 |
| Eam1104I | CTCTTC | 1 cut(s) | 88 |
| EarI | CTCTTC | 1 cut(s) | 88 |
| Eco24I | GRGCYC | 1 cut(s) | 313 |
| Eco57I | CTGAAG | 1 cut(s) | 69 |
| EcoRII | CCWGG | 1 cut(s) | 293 |
| EcoT22I | ATGCAT | 2 cut(s) | 38, 42 |
| EcoT38I | GRGCYC | 1 cut(s) | 313 |
| FaeI | CATG | 8 cut(s) | 26, 36, 40, 57, 125, 304, 450, 558 |
| FaiI | YATR | 9 cut(s) | 24, 34, 38, 55, 123, 302, 448, 494, 556 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 400, 432 |
| FatI | CATG | 8 cut(s) | 22, 32, 36, 53, 121, 300, 446, 554 |
| FriOI | GRGCYC | 1 cut(s) | 313 |
| FspBI | CTAG | 2 cut(s) | 12, 90 |
| HgaI | GACGC | 1 cut(s) | 371 |
| Hin1II | CATG | 8 cut(s) | 26, 36, 40, 57, 125, 304, 450, 558 |
| HinfI | GANTC | 2 cut(s) | 241, 442 |
| HphI | GGTGA | 1 cut(s) | 479 |
| Hpy166II | GTNNAC | 1 cut(s) | 148 |
| Hpy188III | TCNNGA | 5 cut(s) | 112, 206, 301, 478, 550 |
| Hpy8I | GTNNAC | 1 cut(s) | 148 |
| HpyAV | CCTTC | 2 cut(s) | 212, 335 |
| HpyCH4III | ACNGT | 1 cut(s) | 547 |
| HpyCH4IV | ACGT | 2 cut(s) | 224, 246 |
| HpyCH4V | TGCA | 7 cut(s) | 36, 40, 72, 134, 260, 492, 521 |
| HpySE526I | ACGT | 2 cut(s) | 224, 246 |
| Hsp92II | CATG | 8 cut(s) | 26, 36, 40, 57, 125, 304, 450, 558 |
| Kzo9I | GATC | 2 cut(s) | 469, 480 |
| LmnI | GCTCC | 2 cut(s) | 93, 316 |
| LpnPI | CCDG | 7 cut(s) | 51, 142, 246, 280, 307, 450, 531 |
| LweI | GCATC | 1 cut(s) | 388 |
| MaeI | CTAG | 2 cut(s) | 12, 90 |
| MaeII | ACGT | 2 cut(s) | 224, 246 |
| MaeIII | GTNAC | 1 cut(s) | 277 |
| MalI | GATC | 2 cut(s) | 471, 482 |
| MboI | GATC | 2 cut(s) | 469, 480 |
| MboII | GAAGA | 3 cut(s) | 75, 190, 194 |
| MhlI | GDGCHC | 1 cut(s) | 313 |
| MluCI | AATT | 2 cut(s) | 346, 522 |
| MmeI | TCCRAC | 2 cut(s) | 339, 455 |
| MnlI | CCTC | 6 cut(s) | 69, 267, 414, 438, 497, 513 |
| Mph1103I | ATGCAT | 2 cut(s) | 38, 42 |
| MseI | TTAA | 1 cut(s) | 393 |
| MspR9I | CCNGG | 1 cut(s) | 295 |
| Mva1269I | GAATGC | 1 cut(s) | 40 |
| MvaI | CCWGG | 1 cut(s) | 295 |
| NdeII | GATC | 2 cut(s) | 469, 480 |
| NlaIII | CATG | 8 cut(s) | 26, 36, 40, 57, 125, 304, 450, 558 |
| NlaIV | GGNNCC | 1 cut(s) | 312 |
| NsiI | ATGCAT | 2 cut(s) | 38, 42 |
| NspI | RCATGY | 3 cut(s) | 36, 40, 57 |
| PaeI | GCATGC | 2 cut(s) | 36, 40 |
| PagI | TCATGA | 1 cut(s) | 300 |
| PctI | GAATGC | 1 cut(s) | 40 |
| PfeI | GAWTC | 2 cut(s) | 241, 442 |
| Psp6I | CCWGG | 1 cut(s) | 293 |
| PspGI | CCWGG | 1 cut(s) | 293 |
| PspN4I | GGNNCC | 1 cut(s) | 312 |
| RsaI | GTAC | 4 cut(s) | 52, 101, 149, 223 |
| RsaNI | GTAC | 4 cut(s) | 51, 100, 148, 222 |
| SaqAI | TTAA | 1 cut(s) | 393 |
| Sau3AI | GATC | 2 cut(s) | 469, 480 |
| ScrFI | CCNGG | 1 cut(s) | 295 |
| SduI | GDGCHC | 1 cut(s) | 313 |
| SfaNI | GCATC | 1 cut(s) | 388 |
| SfcI | CTRYAG | 1 cut(s) | 543 |
| SmlI | CTYRAG | 2 cut(s) | 353, 476 |
| SmoI | CTYRAG | 2 cut(s) | 353, 476 |
| SphI | GCATGC | 2 cut(s) | 36, 40 |
| Sse9I | AATT | 2 cut(s) | 346, 522 |
| SspMI | CTAG | 2 cut(s) | 12, 90 |
| StyD4I | CCNGG | 1 cut(s) | 293 |
| TaaI | ACNGT | 1 cut(s) | 547 |
| TaiI | ACGT | 2 cut(s) | 227, 249 |
| TaqI | TCGA | 2 cut(s) | 483, 535 |
| TasI | AATT | 2 cut(s) | 346, 522 |
| TatI | WGTACW | 3 cut(s) | 50, 99, 147 |
| TfiI | GAWTC | 2 cut(s) | 241, 442 |
| Tru1I | TTAA | 1 cut(s) | 393 |
| Tru9I | TTAA | 1 cut(s) | 393 |
| TspDTI | ATGAA | 4 cut(s) | 39, 231, 289, 317 |
| TspGWI | ACGGA | 1 cut(s) | 154 |
| XapI | RAATTY | 1 cut(s) | 346 |
| XceI | RCATGY | 3 cut(s) | 36, 40, 57 |
| XspI | CTAG | 2 cut(s) | 12, 90 |
| Zsp2I | ATGCAT | 2 cut(s) | 38, 42 |