E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 962 |
| AccII | CGCG | 1 cut(s) | 41 |
| AciI | CCGC | 2 cut(s) | 41, 1026 |
| AclWI | GGATC | 2 cut(s) | 508, 1050 |
| AcoI | YGGCCR | 1 cut(s) | 1027 |
| AcsI | RAATTY | 1 cut(s) | 764 |
| AcuI | CTGAAG | 1 cut(s) | 670 |
| AfaI | GTAC | 3 cut(s) | 617, 725, 796 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 474, 641, 986 |
| AgsI | TTSAA | 5 cut(s) | 290, 359, 521, 531, 863 |
| AleI | CACNNNNGTG | 1 cut(s) | 120 |
| AluBI | AGCT | 4 cut(s) | 425, 855, 870, 1033 |
| AluI | AGCT | 4 cut(s) | 425, 855, 870, 1033 |
| Alw21I | GWGCWC | 1 cut(s) | 950 |
| AlwI | GGATC | 2 cut(s) | 508, 1050 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 758 |
| AoxI | GGCC | 2 cut(s) | 1027, 1055 |
| ApoI | RAATTY | 1 cut(s) | 764 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 558, 590 |
| AspS9I | GGNCC | 1 cut(s) | 477 |
| AsuC2I | CCSGG | 1 cut(s) | 475 |
| AsuHPI | GGTGA | 2 cut(s) | 116, 143 |
| AvaII | GGWCC | 1 cut(s) | 477 |
| AxyI | CCTNAGG | 1 cut(s) | 1059 |
| BbsI | GAAGAC | 2 cut(s) | 297, 388 |
| Bbv12I | GWGCWC | 1 cut(s) | 950 |
| BccI | CCATC | 6 cut(s) | 65, 194, 236, 395, 1033, 1088 |
| BceAI | ACGGC | 2 cut(s) | 483, 827 |
| BciVI | GTATCC | 1 cut(s) | 29 |
| BclI | TGATCA | 1 cut(s) | 406 |
| BcnI | CCSGG | 1 cut(s) | 475 |
| BfaI | CTAG | 1 cut(s) | 833 |
| BfmI | CTRYAG | 2 cut(s) | 91, 730 |
| BfuI | GTATCC | 1 cut(s) | 29 |
| BisI | GCNGC | 1 cut(s) | 1027 |
| BlpI | GCTNAGC | 1 cut(s) | 851 |
| BlsI | GCNGC | 1 cut(s) | 1028 |
| Bme1390I | CCNGG | 1 cut(s) | 475 |
| Bme18I | GGWCC | 1 cut(s) | 477 |
| BmgT120I | GGNCC | 1 cut(s) | 477 |
| BmiI | GGNNCC | 1 cut(s) | 478 |
| BmrFI | CCNGG | 1 cut(s) | 475 |
| BmsI | GCATC | 3 cut(s) | 20, 447, 551 |
| BpiI | GAAGAC | 2 cut(s) | 297, 388 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 851 |
| BpuEI | CTTGAG | 2 cut(s) | 794, 1019 |
| BpuMI | CCSGG | 1 cut(s) | 475 |
| BsaBI | GATNNNNATC | 5 cut(s) | 117, 144, 174, 204, 234 |
| BsaJI | CCNNGG | 1 cut(s) | 909 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 93, 120, 123, 150 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 474, 641, 986 |
| Bse1I | ACTGG | 1 cut(s) | 740 |
| Bse21I | CCTNAGG | 1 cut(s) | 1059 |
| Bse8I | GATNNNNATC | 5 cut(s) | 117, 144, 174, 204, 234 |
| BseDI | CCNNGG | 1 cut(s) | 909 |
| BseGI | GGATG | 6 cut(s) | 11, 387, 566, 662, 1069, 1080 |
| BseJI | GATNNNNATC | 5 cut(s) | 117, 144, 174, 204, 234 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 474, 641, 986 |
| BseMII | CTCAG | 1 cut(s) | 1050 |
| BseNI | ACTGG | 1 cut(s) | 740 |
| BseRI | GAGGAG | 1 cut(s) | 421 |
| BseYI | CCCAGC | 1 cut(s) | 866 |
| Bsh1236I | CGCG | 1 cut(s) | 41 |
| BshFI | GGCC | 2 cut(s) | 1029, 1057 |
| BsiHKAI | GWGCWC | 1 cut(s) | 950 |
| BsiSI | CCGG | 1 cut(s) | 474 |
| BslFI | GGGAC | 1 cut(s) | 447 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 474, 641, 986 |
| BsmFI | GGGAC | 1 cut(s) | 447 |
| BsmI | GAATGC | 1 cut(s) | 365 |
| BsnI | GGCC | 2 cut(s) | 1029, 1057 |
| Bsp1286I | GDGCHC | 1 cut(s) | 950 |
| Bsp143I | GATC | 8 cut(s) | 85, 112, 139, 169, 229, 406, 513, 1042 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 851 |
| BspACI | CCGC | 2 cut(s) | 41, 1026 |
| BspANI | GGCC | 2 cut(s) | 1029, 1057 |
| BspCNI | CTCAG | 1 cut(s) | 1051 |
| BspFNI | CGCG | 1 cut(s) | 41 |
| BspHI | TCATGA | 1 cut(s) | 148 |
| BspLI | GGNNCC | 1 cut(s) | 478 |
| BspMAI | CTGCAG | 1 cut(s) | 734 |
| BspPI | GGATC | 2 cut(s) | 508, 1050 |
| BsrI | ACTGG | 1 cut(s) | 740 |
| BssECI | CCNNGG | 1 cut(s) | 909 |
| BssMI | GATC | 8 cut(s) | 85, 112, 139, 169, 229, 406, 513, 1042 |
| BssT1I | CCWWGG | 1 cut(s) | 909 |
| Bst6I | CTCTTC | 3 cut(s) | 19, 40, 291 |
| BstC8I | GCNNGC | 2 cut(s) | 427, 1031 |
| BstDEI | CTNAG | 3 cut(s) | 851, 923, 1059 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 984 |
| BstF5I | GGATG | 6 cut(s) | 11, 387, 566, 662, 1069, 1080 |
| BstFNI | CGCG | 1 cut(s) | 41 |
| BstKTI | GATC | 8 cut(s) | 88, 115, 142, 172, 232, 409, 516, 1045 |
| BstMBI | GATC | 8 cut(s) | 85, 112, 139, 169, 229, 406, 513, 1042 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 17, 369, 435 |
| BstSCI | CCNGG | 1 cut(s) | 473 |
| BstSFI | CTRYAG | 2 cut(s) | 91, 730 |
| BstUI | CGCG | 1 cut(s) | 41 |
| BstV2I | GAAGAC | 2 cut(s) | 297, 388 |
| BstX2I | RGATCY | 2 cut(s) | 513, 1042 |
| BstYI | RGATCY | 2 cut(s) | 513, 1042 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1059 |
| BsuI | GTATCC | 1 cut(s) | 29 |
| BsuRI | GGCC | 2 cut(s) | 1029, 1057 |
| BtsCI | GGATG | 6 cut(s) | 11, 387, 566, 662, 1069, 1080 |
| BtsI | GCAGTG | 1 cut(s) | 727 |
| BtsIMutI | CAGTG | 1 cut(s) | 727 |
| Cac8I | GCNNGC | 2 cut(s) | 427, 1031 |
| CaiI | CAGNNNCTG | 1 cut(s) | 758 |
| CciI | TCATGA | 1 cut(s) | 148 |
| Cfr13I | GGNCC | 1 cut(s) | 477 |
| CseI | GACGC | 1 cut(s) | 856 |
| Csp6I | GTAC | 3 cut(s) | 616, 724, 795 |
| CviAII | CATG | 4 cut(s) | 149, 540, 557, 630 |
| CviQI | GTAC | 3 cut(s) | 616, 724, 795 |
| DdeI | CTNAG | 3 cut(s) | 851, 923, 1059 |
| DpnI | GATC | 8 cut(s) | 87, 114, 141, 171, 231, 408, 515, 1044 |
| DpnII | GATC | 8 cut(s) | 85, 112, 139, 169, 229, 406, 513, 1042 |
| EaeI | YGGCCR | 1 cut(s) | 1027 |
| Eam1104I | CTCTTC | 3 cut(s) | 19, 40, 291 |
| EarI | CTCTTC | 3 cut(s) | 19, 40, 291 |
| Eco130I | CCWWGG | 1 cut(s) | 909 |
| Eco47I | GGWCC | 1 cut(s) | 477 |
| Eco57I | CTGAAG | 1 cut(s) | 670 |
| Eco81I | CCTNAGG | 1 cut(s) | 1059 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 984 |
| EcoT14I | CCWWGG | 1 cut(s) | 909 |
| ErhI | CCWWGG | 1 cut(s) | 909 |
| FaeI | CATG | 4 cut(s) | 152, 543, 560, 633 |
| FaqI | GGGAC | 1 cut(s) | 447 |
| FatI | CATG | 4 cut(s) | 148, 539, 556, 629 |
| FauI | CCCGC | 1 cut(s) | 34 |
| FauNDI | CATATG | 1 cut(s) | 1052 |
| FbaI | TGATCA | 1 cut(s) | 406 |
| Fnu4HI | GCNGC | 1 cut(s) | 1027 |
| FokI | GGATG | 5 cut(s) | 374, 573, 649, 1067, 1076 |
| Fsp4HI | GCNGC | 1 cut(s) | 1027 |
| FspBI | CTAG | 1 cut(s) | 833 |
| GluI | GCNGC | 1 cut(s) | 1027 |
| GsaI | CCCAGC | 1 cut(s) | 870 |
| HaeIII | GGCC | 2 cut(s) | 1029, 1057 |
| HapII | CCGG | 1 cut(s) | 474 |
| HgaI | GACGC | 1 cut(s) | 856 |
| Hin1II | CATG | 4 cut(s) | 152, 543, 560, 633 |
| HincII | GTYRAC | 1 cut(s) | 680 |
| HindII | GTYRAC | 1 cut(s) | 680 |
| HinfI | GANTC | 2 cut(s) | 521, 569 |
| HpaII | CCGG | 1 cut(s) | 474 |
| HphI | GGTGA | 2 cut(s) | 116, 143 |
| Hpy166II | GTNNAC | 3 cut(s) | 596, 616, 680 |
| Hpy188I | TCNGA | 1 cut(s) | 319 |
| Hpy188III | TCNNGA | 8 cut(s) | 15, 122, 149, 179, 209, 239, 811, 1036 |
| Hpy8I | GTNNAC | 3 cut(s) | 596, 616, 680 |
| HpyAV | CCTTC | 4 cut(s) | 74, 158, 188, 785 |
| HpyCH4V | TGCA | 6 cut(s) | 438, 503, 564, 732, 902, 1050 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 17, 369, 435 |
| HpyF3I | CTNAG | 3 cut(s) | 851, 923, 1059 |
| Hsp92II | CATG | 4 cut(s) | 152, 543, 560, 633 |
| Ksp22I | TGATCA | 1 cut(s) | 406 |
| Kzo9I | GATC | 8 cut(s) | 85, 112, 139, 169, 229, 406, 513, 1042 |
| LmnI | GCTCC | 3 cut(s) | 434, 476, 875 |
| LweI | GCATC | 3 cut(s) | 20, 447, 551 |
| MaeI | CTAG | 1 cut(s) | 833 |
| MaeIII | GTNAC | 9 cut(s) | 95, 122, 179, 209, 574, 647, 742, 754, 844 |
| MalI | GATC | 8 cut(s) | 87, 114, 141, 171, 231, 408, 515, 1044 |
| MboI | GATC | 8 cut(s) | 85, 112, 139, 169, 229, 406, 513, 1042 |
| MfeI | CAATTG | 1 cut(s) | 504 |
| MflI | RGATCY | 2 cut(s) | 513, 1042 |
| MhlI | GDGCHC | 1 cut(s) | 950 |
| MluCI | AATT | 5 cut(s) | 366, 504, 764, 957, 963 |
| MlyI | GAGTC | 1 cut(s) | 578 |
| MnlI | CCTC | 9 cut(s) | 20, 95, 122, 292, 442, 475, 872, 987, 1054 |
| MseI | TTAA | 2 cut(s) | 825, 998 |
| MslI | CAYNNNNRTG | 2 cut(s) | 120, 147 |
| MspI | CCGG | 1 cut(s) | 474 |
| MspR9I | CCNGG | 1 cut(s) | 475 |
| MunI | CAATTG | 1 cut(s) | 504 |
| Mva1269I | GAATGC | 1 cut(s) | 365 |
| MvnI | CGCG | 1 cut(s) | 41 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 17, 369, 435 |
| NciI | CCSGG | 1 cut(s) | 475 |
| NdeI | CATATG | 1 cut(s) | 1052 |
| NdeII | GATC | 8 cut(s) | 85, 112, 139, 169, 229, 406, 513, 1042 |
| NlaIII | CATG | 4 cut(s) | 152, 543, 560, 633 |
| NlaIV | GGNNCC | 1 cut(s) | 478 |
| NmuCI | GTSAC | 7 cut(s) | 95, 122, 179, 209, 574, 647, 844 |
| OliI | CACNNNNGTG | 1 cut(s) | 120 |
| PagI | TCATGA | 1 cut(s) | 148 |
| PctI | GAATGC | 1 cut(s) | 365 |
| PfeI | GAWTC | 1 cut(s) | 521 |
| PflFI | GACNNNGTC | 1 cut(s) | 653 |
| PkrI | GCNGC | 1 cut(s) | 1028 |
| PleI | GAGTC | 1 cut(s) | 577 |
| PpsI | GAGTC | 1 cut(s) | 577 |
| PsiI | TTATAA | 1 cut(s) | 962 |
| PspFI | CCCAGC | 1 cut(s) | 866 |
| PspN4I | GGNNCC | 1 cut(s) | 478 |
| PspPI | GGNCC | 1 cut(s) | 477 |
| PstI | CTGCAG | 1 cut(s) | 734 |
| PstNI | CAGNNNCTG | 1 cut(s) | 758 |
| PsuI | RGATCY | 2 cut(s) | 513, 1042 |
| PsyI | GACNNNGTC | 1 cut(s) | 653 |
| RsaI | GTAC | 3 cut(s) | 617, 725, 796 |
| RsaNI | GTAC | 3 cut(s) | 616, 724, 795 |
| RseI | CAYNNNNRTG | 2 cut(s) | 120, 147 |
| SaqAI | TTAA | 2 cut(s) | 825, 998 |
| SatI | GCNGC | 1 cut(s) | 1027 |
| Sau3AI | GATC | 8 cut(s) | 85, 112, 139, 169, 229, 406, 513, 1042 |
| Sau96I | GGNCC | 1 cut(s) | 477 |
| SchI | GAGTC | 1 cut(s) | 578 |
| ScrFI | CCNGG | 1 cut(s) | 475 |
| SduI | GDGCHC | 1 cut(s) | 950 |
| SfaNI | GCATC | 3 cut(s) | 20, 447, 551 |
| SfcI | CTRYAG | 2 cut(s) | 91, 730 |
| SinI | GGWCC | 1 cut(s) | 477 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 120, 147 |
| SmlI | CTYRAG | 2 cut(s) | 809, 1034 |
| SmoI | CTYRAG | 2 cut(s) | 809, 1034 |
| Sse9I | AATT | 5 cut(s) | 366, 504, 764, 957, 963 |
| SsiI | CCGC | 2 cut(s) | 41, 1026 |
| SspMI | CTAG | 1 cut(s) | 833 |
| StyD4I | CCNGG | 1 cut(s) | 473 |
| StyI | CCWWGG | 1 cut(s) | 909 |
| TaqI | TCGA | 1 cut(s) | 282 |
| TaqII | GACCGA | 1 cut(s) | 562 |
| TasI | AATT | 5 cut(s) | 366, 504, 764, 957, 963 |
| TatI | WGTACW | 1 cut(s) | 615 |
| TauI | GCSGC | 1 cut(s) | 1029 |
| TfiI | GAWTC | 1 cut(s) | 521 |
| Tru1I | TTAA | 2 cut(s) | 825, 998 |
| Tru9I | TTAA | 2 cut(s) | 825, 998 |
| TscAI | CASTG | 1 cut(s) | 734 |
| TseFI | GTSAC | 7 cut(s) | 95, 122, 179, 209, 574, 647, 844 |
| Tsp45I | GTSAC | 7 cut(s) | 95, 122, 179, 209, 574, 647, 844 |
| TspDTI | ATGAA | 8 cut(s) | 72, 270, 324, 390, 464, 618, 651, 804 |
| TspGWI | ACGGA | 2 cut(s) | 114, 141 |
| TspRI | CASTG | 1 cut(s) | 734 |
| Tth111I | GACNNNGTC | 1 cut(s) | 653 |
| VpaK11BI | GGWCC | 1 cut(s) | 477 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 984 |
| XapI | RAATTY | 1 cut(s) | 764 |
| XspI | CTAG | 1 cut(s) | 833 |