E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1020 |
| AccII | CGCG | 1 cut(s) | 42 |
| AciI | CCGC | 2 cut(s) | 40, 42 |
| AclWI | GGATC | 1 cut(s) | 18 |
| AcoI | YGGCCR | 1 cut(s) | 711 |
| AcsI | RAATTY | 1 cut(s) | 986 |
| AcuI | CTGAAG | 2 cut(s) | 336, 718 |
| AfaI | GTAC | 5 cut(s) | 70, 580, 605, 859, 1009 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 689 |
| AflIII | ACRYGT | 1 cut(s) | 656 |
| AgsI | TTSAA | 5 cut(s) | 128, 887, 923, 946, 1052 |
| AjnI | CCWGG | 2 cut(s) | 80, 397 |
| AluBI | AGCT | 6 cut(s) | 62, 356, 476, 828, 915, 930 |
| AluI | AGCT | 6 cut(s) | 62, 356, 476, 828, 915, 930 |
| Alw26I | GTCTC | 1 cut(s) | 999 |
| AlwI | GGATC | 1 cut(s) | 18 |
| Ama87I | CYCGRG | 1 cut(s) | 57 |
| AoxI | GGCC | 2 cut(s) | 43, 711 |
| ApeKI | GCWGC | 4 cut(s) | 489, 609, 693, 1026 |
| ApoI | RAATTY | 1 cut(s) | 986 |
| AspS9I | GGNCC | 1 cut(s) | 44 |
| AsuHPI | GGTGA | 1 cut(s) | 660 |
| AsuNHI | GCTAGC | 1 cut(s) | 356 |
| AvaI | CYCGRG | 1 cut(s) | 57 |
| BbsI | GAAGAC | 3 cut(s) | 148, 297, 439 |
| BbvI | GCAGC | 4 cut(s) | 476, 596, 680, 1013 |
| BccI | CCATC | 3 cut(s) | 182, 277, 322 |
| BceAI | ACGGC | 1 cut(s) | 622 |
| BciT130I | CCWGG | 2 cut(s) | 82, 399 |
| BclI | TGATCA | 4 cut(s) | 256, 262, 457, 742 |
| BcoDI | GTCTC | 1 cut(s) | 999 |
| BfaI | CTAG | 2 cut(s) | 357, 927 |
| BfmI | CTRYAG | 2 cut(s) | 1019, 1027 |
| BfuAI | ACCTGC | 1 cut(s) | 1020 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 534, 1023 |
| BisI | GCNGC | 5 cut(s) | 43, 490, 610, 694, 1027 |
| BlsI | GCNGC | 5 cut(s) | 44, 491, 611, 695, 1028 |
| BmcAI | AGTACT | 1 cut(s) | 1009 |
| Bme1390I | CCNGG | 2 cut(s) | 82, 399 |
| BmeT110I | CYCGRG | 1 cut(s) | 57 |
| BmgT120I | GGNCC | 1 cut(s) | 44 |
| BmiI | GGNNCC | 1 cut(s) | 538 |
| BmrFI | CCNGG | 2 cut(s) | 82, 399 |
| BmsI | GCATC | 2 cut(s) | 27, 76 |
| BmtI | GCTAGC | 1 cut(s) | 360 |
| BpiI | GAAGAC | 3 cut(s) | 148, 297, 439 |
| BsaBI | GATNNNNATC | 1 cut(s) | 261 |
| BsaI | GGTCTC | 1 cut(s) | 999 |
| BsaJI | CCNNGG | 6 cut(s) | 40, 81, 146, 397, 708, 714 |
| BsaWI | WCCGGW | 1 cut(s) | 12 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 689 |
| Bse1I | ACTGG | 1 cut(s) | 451 |
| Bse8I | GATNNNNATC | 1 cut(s) | 261 |
| BseBI | CCWGG | 2 cut(s) | 82, 399 |
| BseDI | CCNNGG | 6 cut(s) | 40, 81, 146, 397, 708, 714 |
| BseGI | GGATG | 3 cut(s) | 18, 91, 292 |
| BseJI | GATNNNNATC | 1 cut(s) | 261 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 689 |
| BseMII | CTCAG | 1 cut(s) | 104 |
| BseNI | ACTGG | 1 cut(s) | 451 |
| BseRI | GAGGAG | 1 cut(s) | 293 |
| BseXI | GCAGC | 4 cut(s) | 476, 596, 680, 1013 |
| BsgI | GTGCAG | 1 cut(s) | 475 |
| Bsh1236I | CGCG | 1 cut(s) | 42 |
| BshFI | GGCC | 2 cut(s) | 45, 713 |
| BsiHKCI | CYCGRG | 1 cut(s) | 57 |
| BsiSI | CCGG | 1 cut(s) | 13 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 689 |
| BsmAI | GTCTC | 1 cut(s) | 999 |
| BsnI | GGCC | 2 cut(s) | 45, 713 |
| Bso31I | GGTCTC | 1 cut(s) | 999 |
| BsoBI | CYCGRG | 1 cut(s) | 57 |
| Bsp143I | GATC | 7 cut(s) | 6, 23, 256, 262, 457, 742, 952 |
| BspACI | CCGC | 2 cut(s) | 40, 42 |
| BspANI | GGCC | 2 cut(s) | 45, 713 |
| BspCNI | CTCAG | 1 cut(s) | 103 |
| BspFNI | CGCG | 1 cut(s) | 42 |
| BspHI | TCATGA | 2 cut(s) | 259, 265 |
| BspLI | GGNNCC | 1 cut(s) | 538 |
| BspMAI | CTGCAG | 2 cut(s) | 1023, 1031 |
| BspMI | ACCTGC | 1 cut(s) | 1020 |
| BspOI | GCTAGC | 1 cut(s) | 360 |
| BspPI | GGATC | 1 cut(s) | 18 |
| BspQI | GCTCTTC | 1 cut(s) | 1047 |
| BspTNI | GGTCTC | 1 cut(s) | 999 |
| BsrI | ACTGG | 1 cut(s) | 451 |
| BssECI | CCNNGG | 6 cut(s) | 40, 81, 146, 397, 708, 714 |
| BssMI | GATC | 7 cut(s) | 6, 23, 256, 262, 457, 742, 952 |
| BssT1I | CCWWGG | 1 cut(s) | 714 |
| Bst2UI | CCWGG | 2 cut(s) | 82, 399 |
| Bst4CI | ACNGT | 3 cut(s) | 439, 797, 910 |
| Bst6I | CTCTTC | 1 cut(s) | 1047 |
| BstC8I | GCNNGC | 3 cut(s) | 358, 478, 1019 |
| BstDEI | CTNAG | 1 cut(s) | 90 |
| BstDSI | CCRYGG | 1 cut(s) | 40 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 687 |
| BstF5I | GGATG | 3 cut(s) | 18, 91, 292 |
| BstFNI | CGCG | 1 cut(s) | 42 |
| BstKTI | GATC | 7 cut(s) | 9, 26, 259, 265, 460, 745, 955 |
| BstMAI | GTCTC | 1 cut(s) | 999 |
| BstMBI | GATC | 7 cut(s) | 6, 23, 256, 262, 457, 742, 952 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 366, 420, 486, 534, 1023 |
| BstNI | CCWGG | 2 cut(s) | 82, 399 |
| BstNSI | RCATGY | 1 cut(s) | 660 |
| BstSCI | CCNGG | 2 cut(s) | 80, 397 |
| BstSFI | CTRYAG | 2 cut(s) | 1019, 1027 |
| BstUI | CGCG | 1 cut(s) | 42 |
| BstV1I | GCAGC | 4 cut(s) | 476, 596, 680, 1013 |
| BstV2I | GAAGAC | 3 cut(s) | 148, 297, 439 |
| BsuRI | GGCC | 2 cut(s) | 45, 713 |
| BtgI | CCRYGG | 1 cut(s) | 40 |
| BtsCI | GGATG | 3 cut(s) | 18, 91, 292 |
| BtsIMutI | CAGTG | 1 cut(s) | 458 |
| BveI | ACCTGC | 1 cut(s) | 1020 |
| Cac8I | GCNNGC | 3 cut(s) | 358, 478, 1019 |
| CciI | TCATGA | 2 cut(s) | 259, 265 |
| Cfr13I | GGNCC | 1 cut(s) | 44 |
| Cfr42I | CCGCGG | 1 cut(s) | 43 |
| Csp6I | GTAC | 5 cut(s) | 69, 579, 604, 858, 1008 |
| CviAII | CATG | 7 cut(s) | 260, 266, 588, 647, 657, 678, 934 |
| CviQI | GTAC | 5 cut(s) | 69, 579, 604, 858, 1008 |
| DdeI | CTNAG | 1 cut(s) | 90 |
| DpnI | GATC | 7 cut(s) | 8, 25, 258, 264, 459, 744, 954 |
| DpnII | GATC | 7 cut(s) | 6, 23, 256, 262, 457, 742, 952 |
| EaeI | YGGCCR | 1 cut(s) | 711 |
| Eam1104I | CTCTTC | 1 cut(s) | 1047 |
| EarI | CTCTTC | 1 cut(s) | 1047 |
| Eco130I | CCWWGG | 1 cut(s) | 714 |
| Eco31I | GGTCTC | 1 cut(s) | 999 |
| Eco57I | CTGAAG | 2 cut(s) | 336, 718 |
| Eco88I | CYCGRG | 1 cut(s) | 57 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 687 |
| EcoRII | CCWGG | 2 cut(s) | 80, 397 |
| EcoT14I | CCWWGG | 1 cut(s) | 714 |
| ErhI | CCWWGG | 1 cut(s) | 714 |
| FaeI | CATG | 7 cut(s) | 263, 269, 591, 650, 660, 681, 937 |
| FatI | CATG | 7 cut(s) | 259, 265, 587, 646, 656, 677, 933 |
| FbaI | TGATCA | 4 cut(s) | 256, 262, 457, 742 |
| Fnu4HI | GCNGC | 5 cut(s) | 43, 490, 610, 694, 1027 |
| FokI | GGATG | 3 cut(s) | 5, 98, 299 |
| Fsp4HI | GCNGC | 5 cut(s) | 43, 490, 610, 694, 1027 |
| FspBI | CTAG | 2 cut(s) | 357, 927 |
| GluI | GCNGC | 5 cut(s) | 43, 490, 610, 694, 1027 |
| HaeIII | GGCC | 2 cut(s) | 45, 713 |
| HapII | CCGG | 1 cut(s) | 13 |
| Hin1II | CATG | 7 cut(s) | 263, 269, 591, 650, 660, 681, 937 |
| HinfI | GANTC | 5 cut(s) | 50, 170, 206, 572, 752 |
| HpaII | CCGG | 1 cut(s) | 13 |
| HphI | GGTGA | 1 cut(s) | 660 |
| Hpy166II | GTNNAC | 2 cut(s) | 428, 668 |
| Hpy188I | TCNGA | 5 cut(s) | 23, 93, 175, 211, 999 |
| Hpy188III | TCNNGA | 4 cut(s) | 260, 266, 842, 1052 |
| Hpy8I | GTNNAC | 2 cut(s) | 428, 668 |
| HpyAV | CCTTC | 3 cut(s) | 170, 359, 1058 |
| HpyCH4III | ACNGT | 3 cut(s) | 439, 797, 910 |
| HpyCH4V | TGCA | 6 cut(s) | 492, 591, 612, 696, 1021, 1029 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 366, 420, 486, 534, 1023 |
| HpyF3I | CTNAG | 1 cut(s) | 90 |
| Hsp92II | CATG | 7 cut(s) | 263, 269, 591, 650, 660, 681, 937 |
| Ksp22I | TGATCA | 4 cut(s) | 256, 262, 457, 742 |
| KspI | CCGCGG | 1 cut(s) | 43 |
| Kzo9I | GATC | 7 cut(s) | 6, 23, 256, 262, 457, 742, 952 |
| LguI | GCTCTTC | 1 cut(s) | 1047 |
| LmnI | GCTCC | 3 cut(s) | 15, 485, 542 |
| Lsp1109I | GCAGC | 4 cut(s) | 476, 596, 680, 1013 |
| LweI | GCATC | 2 cut(s) | 27, 76 |
| MaeI | CTAG | 2 cut(s) | 357, 927 |
| MaeIII | GTNAC | 2 cut(s) | 622, 904 |
| MalI | GATC | 7 cut(s) | 8, 25, 258, 264, 459, 744, 954 |
| MboI | GATC | 7 cut(s) | 6, 23, 256, 262, 457, 742, 952 |
| MluCI | AATT | 4 cut(s) | 417, 778, 804, 986 |
| MnlI | CCTC | 9 cut(s) | 37, 95, 156, 157, 175, 271, 718, 881, 931 |
| MseI | TTAA | 4 cut(s) | 137, 389, 1034, 1066 |
| MslI | CAYNNNNRTG | 3 cut(s) | 264, 270, 661 |
| MspA1I | CMGCKG | 1 cut(s) | 42 |
| MspI | CCGG | 1 cut(s) | 13 |
| MspR9I | CCNGG | 2 cut(s) | 82, 399 |
| MvaI | CCWGG | 2 cut(s) | 82, 399 |
| MvnI | CGCG | 1 cut(s) | 42 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 366, 420, 486, 534, 1023 |
| NdeII | GATC | 7 cut(s) | 6, 23, 256, 262, 457, 742, 952 |
| NheI | GCTAGC | 1 cut(s) | 356 |
| NlaIII | CATG | 7 cut(s) | 263, 269, 591, 650, 660, 681, 937 |
| NlaIV | GGNNCC | 1 cut(s) | 538 |
| NmeAIII | GCCGAG | 2 cut(s) | 575, 689 |
| NmuCI | GTSAC | 2 cut(s) | 622, 904 |
| NspI | RCATGY | 1 cut(s) | 660 |
| PaeR7I | CTCGAG | 1 cut(s) | 57 |
| PagI | TCATGA | 2 cut(s) | 259, 265 |
| PciI | ACATGT | 1 cut(s) | 656 |
| PciSI | GCTCTTC | 1 cut(s) | 1047 |
| PfeI | GAWTC | 5 cut(s) | 50, 170, 206, 572, 752 |
| PkrI | GCNGC | 5 cut(s) | 44, 491, 611, 695, 1028 |
| PscI | ACATGT | 1 cut(s) | 656 |
| Psp6I | CCWGG | 2 cut(s) | 80, 397 |
| PspGI | CCWGG | 2 cut(s) | 80, 397 |
| PspN4I | GGNNCC | 1 cut(s) | 538 |
| PspPI | GGNCC | 1 cut(s) | 44 |
| PspXI | VCTCGAGB | 1 cut(s) | 57 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 61, 93 |
| PstI | CTGCAG | 2 cut(s) | 1023, 1031 |
| RsaI | GTAC | 5 cut(s) | 70, 580, 605, 859, 1009 |
| RsaNI | GTAC | 5 cut(s) | 69, 579, 604, 858, 1008 |
| RseI | CAYNNNNRTG | 3 cut(s) | 264, 270, 661 |
| SacII | CCGCGG | 1 cut(s) | 43 |
| SapI | GCTCTTC | 1 cut(s) | 1047 |
| SaqAI | TTAA | 4 cut(s) | 137, 389, 1034, 1066 |
| SatI | GCNGC | 5 cut(s) | 43, 490, 610, 694, 1027 |
| Sau3AI | GATC | 7 cut(s) | 6, 23, 256, 262, 457, 742, 952 |
| Sau96I | GGNCC | 1 cut(s) | 44 |
| SbfI | CCTGCAGG | 1 cut(s) | 1023 |
| ScaI | AGTACT | 1 cut(s) | 1009 |
| ScrFI | CCNGG | 2 cut(s) | 82, 399 |
| SdaI | CCTGCAGG | 1 cut(s) | 1023 |
| SfaNI | GCATC | 2 cut(s) | 27, 76 |
| SfcI | CTRYAG | 2 cut(s) | 1019, 1027 |
| Sfr274I | CTCGAG | 1 cut(s) | 57 |
| Sfr303I | CCGCGG | 1 cut(s) | 43 |
| SgrBI | CCGCGG | 1 cut(s) | 43 |
| SlaI | CTCGAG | 1 cut(s) | 57 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 264, 270, 661 |
| SmlI | CTYRAG | 1 cut(s) | 57 |
| SmoI | CTYRAG | 1 cut(s) | 57 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1023 |
| Sse9I | AATT | 4 cut(s) | 417, 778, 804, 986 |
| SsiI | CCGC | 2 cut(s) | 40, 42 |
| SspI | AATATT | 2 cut(s) | 376, 616 |
| SspMI | CTAG | 2 cut(s) | 357, 927 |
| StyD4I | CCNGG | 2 cut(s) | 80, 397 |
| StyI | CCWWGG | 1 cut(s) | 714 |
| TaaI | ACNGT | 3 cut(s) | 439, 797, 910 |
| TaqI | TCGA | 5 cut(s) | 5, 58, 113, 347, 841 |
| TaqII | GACCGA | 1 cut(s) | 981 |
| TasI | AATT | 4 cut(s) | 417, 778, 804, 986 |
| TatI | WGTACW | 3 cut(s) | 68, 578, 1007 |
| TauI | GCSGC | 1 cut(s) | 45 |
| TfiI | GAWTC | 5 cut(s) | 50, 170, 206, 572, 752 |
| Tru1I | TTAA | 4 cut(s) | 137, 389, 1034, 1066 |
| Tru9I | TTAA | 4 cut(s) | 137, 389, 1034, 1066 |
| TscAI | CASTG | 1 cut(s) | 458 |
| TseFI | GTSAC | 2 cut(s) | 622, 904 |
| TseI | GCWGC | 4 cut(s) | 489, 609, 693, 1026 |
| Tsp45I | GTSAC | 2 cut(s) | 622, 904 |
| TspDTI | ATGAA | 5 cut(s) | 303, 657, 663, 666, 922 |
| TspRI | CASTG | 1 cut(s) | 458 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 687 |
| XapI | RAATTY | 1 cut(s) | 986 |
| XceI | RCATGY | 1 cut(s) | 660 |
| XhoI | CTCGAG | 1 cut(s) | 57 |
| XspI | CTAG | 2 cut(s) | 357, 927 |
| ZrmI | AGTACT | 1 cut(s) | 1009 |